LOCUS       NZ_JALAUL010000017     10442 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus atrophaeus strain CK4F1-2 Contig_0000017, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAUL010000017
VERSION     NZ_JALAUL010000017.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 219.01779805621194x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:32:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,147
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 3,904
            CDSs (with protein)               :: 3,904
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 97 of 165
            Pseudo Genes (incomplete)         :: 106 of 165
            Pseudo Genes (internal stop)      :: 39 of 165
            Pseudo Genes (multiple problems)  :: 66 of 165
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:35:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10442
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAUL010000017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..3532
                     /locus_tag="MOE42_RS20520"
                     /old_locus_tag="MOE42_20465"
     source          1..10442
                     /db_xref="taxon:1452"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus atrophaeus"
                     /strain="CK4F1-2"
                     /submitter_seqid="Contig_0000017"
     region          1..10442
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10442
                     /SMILES="NC([*])C(=O)N1C(CCC1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10442
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<2:>10442](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <3..>10442
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             <3..3532
                     /NRPS_PKS="Domain: AMP-binding (129-525). E-value: 3e-112.
                     Score: 367.2. Matches aSDomain:
                     nrpspksdomains_MOE42_RS20520_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (630-698). E-value: 6.5e-24. Score:
                     75.9. Matches aSDomain: nrpspksdomains_MOE42_RS20520_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (708-1010). E-value:
                     2e-162. Score: 533.7. Matches aSDomain:
                     nrpspksdomains_MOE42_RS20520_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1015-1169). E-value: 3.6e-49.
                     Score: 158.5. Matches aSDomain:
                     nrpspksdomains_MOE42_RS20520_TIGR01720.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390540.1"
                     /locus_tag="MOE42_RS20520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE42_20465"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268487122.1"
                     /sec_met_domain="AMP-binding (E-value: 6.4e-118, bitscore:
                     380.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 8.3e-16, bitscore:
                     45.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.3e-79, bitscore:
                     254.5, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YRDTSRNPLFDVMFALQNIDHENLMLEDLRLRSADIEHKVSKFDL
                     TLYASEEPKGELRFHLEYNTDLYKKKTVEQWLKYLIHIFREITEDHSVTLGKINIVDED
                     ETRLIIHEFNRTKMEYPRHETVSRLFERQAAKTPDAAAVVSHGQTLTYHELNNRANRIA
                     AVLRAKGVGPESVVALLTKRTPELAAGILGILKAGGAYLPIGEDTPSERIEWMLSDSGA
                     GILLQSEKLGNDFLNIEGEYEQIFIEAIQEQGNDTENLESLAGPDSLAYIIYTSGSTGK
                     PKGVMIEQRSVIRLVTNSNYIEVTPSDRLLMTSSFGFDVTTFEIFGPLLNGAALYMADK
                     ETFLDSHQLETYIHQNGITTLWLTSPLFNHLTEQNENTFAGLSTLIIGGEALSASHVNR
                     IRKACPSLAIWNGYGPTENTTFSTCFEIERYYEHSIPIGRPIGNSTAYILNNWGMLQPI
                     GTVGELCVGGDGVARGYLGRPELTKEKFVPNPFAIGERMYRTGDLACWLPDGTIEYAGR
                     MDEQVKIRGYRVELGEIETAIRHIDGVKEAAVLARPGQSGSKELVAYMSLQADMDAEKV
                     RSQLSSTLPGYMIPVYMIEMDALPLTANGKLDRKALPEPELSSKSKYVQPRSELEEQLA
                     LIWQEVLGLEKIGIEDSFFELGGDSIKALQVSARLGRYGWSMTASDLFRHPRIKDVSAV
                     IRKTERVIDQGPVKGEVPLTPVQHWFLTQRITDRHHFNQSVMLFNSGRLIEKPLRETLQ
                     KLAEHHDALRMIYRKESGHVIQINQDIHETELYTLNASDFSDTEAWENEIEKEVAALQR
                     GMNIEEGPLLRAGWFKTPSGDYLFLTIHHLVVDGVSWRTLLEDLSAGYHQAAAGQSIQL
                     PPKTDSYKEYAERLQDYAQSSKLIQEEAYWRSVEEETIAALPYEKPQHNDTNDSSQNTV
                     RFTLSEEDTTVLLQKVNHAYGTDTQDILLTAAALAIRDWTGESKLRIAMEGHGRENILP
                     NVDISRTVGWFTSMYPVLLEIQKESGELGTAVKTVKDTLGRIPQKGTGYGILKYITKPE
                     NKSIQFGGAPEIGFNYLGQFNDTEQQETFSLSGLASGEDITRSWQREQIIEISALAAEN
                     KLHFNLSYPPNRFYRKTMEHLLNGIQNYLQSIMKHCAGKQETEKTLSDFSSKSLTMEDL
                     DSISDLVQEL"
     aSDomain        392..1579
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20520_AMP-binding.1"
                     /evalue="3.00E-112"
                     /label="MOE42_RS20520_AMP-binding.1"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="525"
                     /protein_start="129"
                     /score="367.2"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FERQAAKTPDAAAVVSHGQTLTYHELNNRANRIAAVLRAKGVGPE
                     SVVALLTKRTPELAAGILGILKAGGAYLPIGEDTPSERIEWMLSDSGAGILLQSEKLGN
                     DFLNIEGEYEQIFIEAIQEQGNDTENLESLAGPDSLAYIIYTSGSTGKPKGVMIEQRSV
                     IRLVTNSNYIEVTPSDRLLMTSSFGFDVTTFEIFGPLLNGAALYMADKETFLDSHQLET
                     YIHQNGITTLWLTSPLFNHLTEQNENTFAGLSTLIIGGEALSASHVNRIRKACPSLAIW
                     NGYGPTENTTFSTCFEIERYYEHSIPIGRPIGNSTAYILNNWGMLQPIGTVGELCVGGD
                     GVARGYLGRPELTKEKFVPNPFAIGERMYRTGDLACWLPDGTIEYAGRMDEQVKIR"
     aSModule        392..3511
                     /complete
                     /domains="nrpspksdomains_MOE42_RS20520_AMP-binding.1"
                     /domains="nrpspksdomains_MOE42_RS20520_PCP.1"
                     /domains="nrpspksdomains_MOE42_RS20520_Epimerization.1"
                     /domains="nrpspksdomains_MOE42_RS20520_TIGR01720.1"
                     /locus_tags="MOE42_RS20520"
                     /monomer_pairings="X -> D-X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       578..616
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0001"
                     /evalue="1.50E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="204"
                     /protein_start="191"
                     /score="15.1"
                     /tool="antismash"
                     /translation="LGILKAGGAYLPI"
     CDS_motif       812..868
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0002"
                     /evalue="9.90E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="288"
                     /protein_start="269"
                     /score="33.4"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGKPKGVMIE"
     CDS_motif       1373..1462
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0003"
                     /evalue="4.40E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="486"
                     /protein_start="456"
                     /score="57.3"
                     /tool="antismash"
                     /translation="PIGTVGELCVGGDGVARGYLGRPELTKEKF"
     CDS_motif       1544..1609
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0004"
                     /evalue="5.90E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="535"
                     /protein_start="513"
                     /score="37.5"
                     /tool="antismash"
                     /translation="YAGRMDEQVKIRGYRVELGEIE"
     aSDomain        1895..2098
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20520_PCP.1"
                     /evalue="6.50E-24"
                     /label="MOE42_RS20520_PCP.1"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="698"
                     /protein_start="630"
                     /score="75.9"
                     /tool="antismash"
                     /translation="EEQLALIWQEVLGLEKIGIEDSFFELGGDSIKALQVSARLGRYGW
                     SMTASDLFRHPRIKDVSAVIRKT"
     aSDomain        2129..3034
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20520_Epimerization.1"
                     /evalue="2.00E-162"
                     /label="MOE42_RS20520_Epimerization.1"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="1010"
                     /protein_start="708"
                     /score="533.7"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="GEVPLTPVQHWFLTQRITDRHHFNQSVMLFNSGRLIEKPLRETLQ
                     KLAEHHDALRMIYRKESGHVIQINQDIHETELYTLNASDFSDTEAWENEIEKEVAALQR
                     GMNIEEGPLLRAGWFKTPSGDYLFLTIHHLVVDGVSWRTLLEDLSAGYHQAAAGQSIQL
                     PPKTDSYKEYAERLQDYAQSSKLIQEEAYWRSVEEETIAALPYEKPQHNDTNDSSQNTV
                     RFTLSEEDTTVLLQKVNHAYGTDTQDILLTAAALAIRDWTGESKLRIAMEGHGRENILP
                     NVDISRTVGWFTSMYPVLLEI"
     CDS_motif       2141..2167
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0005"
                     /evalue="4.30E-03"
                     /label="NRPS-E1"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="721"
                     /protein_start="712"
                     /score="10.3"
                     /tool="antismash"
                     /translation="LTPVQHWFL"
     CDS_motif       2507..2572
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0006"
                     /evalue="3.50E-12"
                     /label="NRPS-E2"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="856"
                     /protein_start="834"
                     /score="38.4"
                     /tool="antismash"
                     /translation="LFLTIHHLVVDGVSWRTLLEDL"
     CDS_motif       2978..3028
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0007"
                     /evalue="1.00E-09"
                     /label="NRPS-E5"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="1008"
                     /protein_start="991"
                     /score="30.2"
                     /tool="antismash"
                     /translation="DISRTVGWFTSMYPVLL"
     aSDomain        3050..3511
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20520_TIGR01720.1"
                     /evalue="3.60E-49"
                     /label="MOE42_RS20520_TIGR01720.1"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="1169"
                     /protein_start="1015"
                     /score="158.5"
                     /tool="antismash"
                     /translation="ELGTAVKTVKDTLGRIPQKGTGYGILKYITKPENKSIQFGGAPEI
                     GFNYLGQFNDTEQQETFSLSGLASGEDITRSWQREQIIEISALAAENKLHFNLSYPPNR
                     FYRKTMEHLLNGIQNYLQSIMKHCAGKQETEKTLSDFSSKSLTMEDLDSI"
     CDS_motif       3074..3133
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0008"
                     /evalue="4.30E-08"
                     /label="NRPS-E6"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="1043"
                     /protein_start="1023"
                     /score="25.4"
                     /tool="antismash"
                     /translation="VKDTLGRIPQKGTGYGILKY"
     CDS_motif       3176..3205
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20520_0009"
                     /evalue="5.90E-03"
                     /label="NRPS-E7"
                     /locus_tag="MOE42_RS20520"
                     /protein_end="1067"
                     /protein_start="1057"
                     /score="9.3"
                     /tool="antismash"
                     /translation="PEIGFNYLGQ"
     aSDomain        3558..3647
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20525_NRPS-COM_Nterm.1"
                     /evalue="3.10E-18"
                     /label="MOE42_RS20525_NRPS-COM_Nterm.1"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="30"
                     /protein_start="0"
                     /score="56.7"
                     /tool="antismash"
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLHEDS"
     gene            3558..>10442
                     /locus_tag="MOE42_RS20525"
                     /old_locus_tag="MOE42_20470"
                     /pseudo=""
     CDS             3558..>10442
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-30). E-value: 3.1e-18.
                     Score: 56.7. Matches aSDomain:
                     nrpspksdomains_MOE42_RS20525_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-306). E-value:
                     3.5e-115. Score: 376.2. Matches aSDomain:
                     nrpspksdomains_MOE42_RS20525_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-872). E-value:
                     1.3e-105. Score: 345.2. Matches aSDomain:
                     nrpspksdomains_MOE42_RS20525_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (978-1047). E-value: 5.4e-24. Score:
                     76.2. Matches aSDomain: nrpspksdomains_MOE42_RS20525_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1061-1341). E-value:
                     9.4e-103. Score: 335.2. Matches aSDomain:
                     nrpspksdomains_MOE42_RS20525_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1507-1908). E-value:
                     2.5e-113. Score: 370.7. Matches aSDomain:
                     nrpspksdomains_MOE42_RS20525_AMP-binding.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330630.1"
                     /locus_tag="MOE42_RS20525"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="MOE42_20470"
                     /product="amino acid adenylation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="PP-binding (E-value: 1.7e-16, bitscore:
                     47.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.7e-91, bitscore:
                     292.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.5e-124, bitscore:
                     401.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLHEDSRAYFEQAVFSIAGEV
                     HSRFFQDSINALVARHDIFRTIFINQNVSAPQQVVLRDRTVTVQEEDISHLDESRQSEF
                     IENYKEKDREKGFNLQKDVLMRISLLKTGKQQYTCLWSFHHIVMDGWCFSIVLKEFFQI
                     YAAHINASPLTLEPVRPYGSYINWLMEQDKEQAITYWKSYLAGYEQQTALPKQNKTKDK
                     QKSGQAHVTFSFSKKDSSRLSEMAVKEEVTLSTLFHTLWGILLQKYNNTDDAVFGSVTS
                     GRPSSIEGIENMVGLFINTIPVRVQGADTPVSELMKRMQKEALDAEAHSYHPLYEIQAH
                     SAVKQGLIDHILVFENYPVQNEIDMLNSHDDAQELFQIHHFSVADETNYSFYLMVAPGD
                     EIHIKMSYDSGVYDRSFILGVKGHLTHMVSQILDNPDISPAGIDITTSSEKHLLLGKKE
                     ADAPAFQTIHSMFEKQAELTPNMQAVTYQGRSITYRELNENANKLARILRKRGVRRQEP
                     VAIMAERSPELTAGILGILKAGGSLVPIDPNYPPERIRFILQDSCCSHLVSHRSLASLL
                     ESVNEEALRTVTYTEESAQEEDGSNVKPVNTADDLLYTIYTSGTTGKPKGVQLEHRSMA
                     NLLHFQFTNSGINFRTNVLQYATPAFDVCYQEIFSTLAGGGTVHIVPEPIKRDVSQLFA
                     FIADHNAEIVFFPTAFIKMMFSEEEYANAFPDGVKHLITAGEQLTISQLFQQVLRRHNT
                     VLHNHYGPSETHVVSAYSIHPGEHIPESPPIGKPIDHTHMYILNGQKQLQPKGIAGELY
                     ISGSSVARGYFRNEELTREKFLPDPFRPGATMFRTGDMARLSEDGNIEYIGRTDDQVKI
                     RGYRVEPGEIEMTLQNHPKIKEAAVIIRQDHAGENELCAYYSAPNRLNSLALRRYLAEE
                     MPEYMLPLKWGWVESIPLTPNGKVDKRSLPEPEAAADDKQYAAPRNLTELKLAQLWEDV
                     LKDGPVGIRDNFFDRGGHSLKATALVSRIAKEVGVKVPLQEVFAHPTVEGLAAVVREAE
                     ENPYAAIEPAPEQDVYPVSSAQKRMYVLQQMEDGGVGYNMPAVLELEGKLDRGRLETVF
                     KKLISRHEPLRTSFEEGTDGEPVQRIHSKITFALCEQEKTSAFIRPFDLSKAPLLRAGL
                     IKETDERHLLLVDMHHIISDGVSVHTLIREFGDLYAHRTLEPLRIQYKDYAVWQQEFKS
                     GGTYQKQEAYWLNQLSGELPVLELPTDKLRPAVRTFAGDKVSFIISKEITSGLKQLARK
                     NECTLYMTLLAAYTAFLARLSGQEEIVVGSPIAGRPYADLESILGMFVNTLALRTHPEG
                     GKRFSEYLQEVRQTALEAYEHQDYPFEELVDMLGVQRDMSRNPLFDTTLTLQNMEQQKL
                     QLDGLQLKTADVDHPISKFDISLYVMENEEELYCQFEYSTSLFEKETIQRWSRFFLRLV
                     ENTVSSPDTELDDISVLTKEEEKTFIQSFSPLPTTSFPLAQTLHHQLEKQAAKTPERPA
                     VFYGEEVISYKELNERANRVAWELIERGVTSETVIAITGRRSPEMLIGIYAILKAGGAY
                     VPIDPEYPEERIRFLLKDSGAKLLLVESGSFEIPSFEGDVLDLNINVSREKNHLINPDV
                     SVRSNSLAYIIYTSGSTGTPKGVQIEHQSAVNFLQSLQTRYPLNESDMILHKTSYSFDA
                     SIWELFWWPYAGASVFLLPQGGEKDPEMMIKVIEEKRITAAHFVPSMLHAFLAYVKHRA
                     VPIVPNGLKRVFAGGEQLGAHLVSRFYELLPGVELTNSYGPTETTVEAAYFDCPLGKDL
                     KRIPIGKPIHNLRLYILNKKKQILPTGCTGELYIAGNGVARGYLNRPELTTERFLSDPF
                     YPGERMYKTGDLARWLPDGQVEFLGRLDDQVKIRGYRIEPGEIESALRNLEGVQEAAVI
                     TRADSGEAELCAYIQGRDKNDVRAQLALVLPGYMIPAHFVQIDTWPITPSGKLDQKALP
                     APDGAVGQESYKAPRNLTEMKLAQLWEYXRYHGRLFRPGRPLVKSNGAHLPNRQDIRRP
                     SAA"
     aSDomain        3579..4475
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20525_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="3.50E-115"
                     /label="MOE42_RS20525_Condensation_DCL.1"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="306"
                     /protein_start="7"
                     /score="376.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVMDG"
                     /tool="antismash"
                     /translation="QDIYPLSYMQEGMLFHSLLHEDSRAYFEQAVFSIAGEVHSRFFQD
                     SINALVARHDIFRTIFINQNVSAPQQVVLRDRTVTVQEEDISHLDESRQSEFIENYKEK
                     DREKGFNLQKDVLMRISLLKTGKQQYTCLWSFHHIVMDGWCFSIVLKEFFQIYAAHINA
                     SPLTLEPVRPYGSYINWLMEQDKEQAITYWKSYLAGYEQQTALPKQNKTKDKQKSGQAH
                     VTFSFSKKDSSRLSEMAVKEEVTLSTLFHTLWGILLQKYNNTDDAVFGSVTSGRPSSIE
                     GIENMVGLFINTIPVRVQ"
     aSModule        3579..6698
                     /complete
                     /domains="nrpspksdomains_MOE42_RS20525_Condensation_DCL.1"
                     /domains="nrpspksdomains_MOE42_RS20525_AMP-binding.1"
                     /domains="nrpspksdomains_MOE42_RS20525_PCP.1"
                     /locus_tags="MOE42_RS20525"
                     /monomer_pairings="Pro -> Pro"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       3588..3629
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0001"
                     /evalue="9.70E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="24"
                     /protein_start="10"
                     /score="14.4"
                     /tool="antismash"
                     /translation="YPLSYMQEGMLFHS"
     CDS_motif       3651..3764
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0002"
                     /evalue="5.20E-13"
                     /label="C2_DCL_024-062"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="69"
                     /protein_start="31"
                     /score="41.3"
                     /tool="antismash"
                     /translation="AYFEQAVFSIAGEVHSRFFQDSINALVARHDIFRTIFI"
     CDS_motif       3984..4049
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0003"
                     /evalue="1.70E-12"
                     /label="C3_DCL_135-156"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="164"
                     /protein_start="142"
                     /score="39.7"
                     /tool="antismash"
                     /translation="FHHIVMDGWCFSIVLKEFFQIY"
     CDS_motif       4374..4469
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0004"
                     /evalue="2.10E-18"
                     /label="C5_DCL_263-294"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="304"
                     /protein_start="272"
                     /score="58.7"
                     /tool="antismash"
                     /translation="DAVFGSVTSGRPSSIEGIENMVGLFINTIPVR"
     CDS_motif       4515..4631
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0005"
                     /evalue="1.20E-21"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="358"
                     /protein_start="319"
                     /score="69.2"
                     /tool="antismash"
                     /translation="QKEALDAEAHSYHPLYEIQAHSAVKQGLIDHILVFENYP"
     aSDomain        4968..6173
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20525_AMP-binding.1"
                     /evalue="1.30E-105"
                     /label="MOE42_RS20525_AMP-binding.1"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="872"
                     /protein_start="470"
                     /score="345.2"
                     /specificity="substrate consensus: Pro"
                     /tool="antismash"
                     /translation="FEKQAELTPNMQAVTYQGRSITYRELNENANKLARILRKRGVRRQ
                     EPVAIMAERSPELTAGILGILKAGGSLVPIDPNYPPERIRFILQDSCCSHLVSHRSLAS
                     LLESVNEEALRTVTYTEESAQEEDGSNVKPVNTADDLLYTIYTSGTTGKPKGVQLEHRS
                     MANLLHFQFTNSGINFRTNVLQYATPAFDVCYQEIFSTLAGGGTVHIVPEPIKRDVSQL
                     FAFIADHNAEIVFFPTAFIKMMFSEEEYANAFPDGVKHLITAGEQLTISQLFQQVLRRH
                     NTVLHNHYGPSETHVVSAYSIHPGEHIPESPPIGKPIDHTHMYILNGQKQLQPKGIAGE
                     LYISGSSVARGYFRNEELTREKFLPDPFRPGATMFRTGDMARLSEDGNIEYIGRTDDQV
                     KIR"
     CDS_motif       5154..5195
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0006"
                     /evalue="4.90E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="546"
                     /protein_start="532"
                     /score="10.6"
                     /tool="antismash"
                     /translation="LGILKAGGSLVPID"
     CDS_motif       5394..5450
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0007"
                     /evalue="2.10E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="631"
                     /protein_start="612"
                     /score="26.3"
                     /tool="antismash"
                     /translation="YTIYTSGTTGKPKGVQLEH"
     CDS_motif       5832..5858
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0008"
                     /evalue="4.60E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="767"
                     /protein_start="758"
                     /score="4.1"
                     /tool="antismash"
                     /translation="YGPSETHVV"
     CDS_motif       5967..6056
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0009"
                     /evalue="9.30E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="833"
                     /protein_start="803"
                     /score="40.3"
                     /tool="antismash"
                     /translation="PKGIAGELYISGSSVARGYFRNEELTREKF"
     CDS_motif       6138..6203
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0010"
                     /evalue="2.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="882"
                     /protein_start="860"
                     /score="38.6"
                     /tool="antismash"
                     /translation="YIGRTDDQVKIRGYRVEPGEIE"
     aSDomain        6492..6698
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20525_PCP.1"
                     /evalue="5.40E-24"
                     /label="MOE42_RS20525_PCP.1"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1047"
                     /protein_start="978"
                     /score="76.2"
                     /tool="antismash"
                     /translation="ELKLAQLWEDVLKDGPVGIRDNFFDRGGHSLKATALVSRIAKEVG
                     VKVPLQEVFAHPTVEGLAAVVREA"
     aSDomain        6741..7580
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20525_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="9.40E-103"
                     /label="MOE42_RS20525_Condensation_LCL.1"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1341"
                     /protein_start="1061"
                     /score="335.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="DVYPVSSAQKRMYVLQQMEDGGVGYNMPAVLELEGKLDRGRLETV
                     FKKLISRHEPLRTSFEEGTDGEPVQRIHSKITFALCEQEKTSAFIRPFDLSKAPLLRAG
                     LIKETDERHLLLVDMHHIISDGVSVHTLIREFGDLYAHRTLEPLRIQYKDYAVWQQEFK
                     SGGTYQKQEAYWLNQLSGELPVLELPTDKLRPAVRTFAGDKVSFIISKEITSGLKQLAR
                     KNECTLYMTLLAAYTAFLARLSGQEEIVVGSPIAGRPYADLESILGMFVNTLALRTHP"
     aSModule        6741..9281
                     /domains="nrpspksdomains_MOE42_RS20525_Condensation_LCL.1"
                     /domains="nrpspksdomains_MOE42_RS20525_AMP-binding.2"
                     /incomplete
                     /locus_tags="MOE42_RS20525"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       6813..6920
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0011"
                     /evalue="1.10E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1121"
                     /protein_start="1085"
                     /score="46.8"
                     /tool="antismash"
                     /translation="YNMPAVLELEGKLDRGRLETVFKKLISRHEPLRTSF"
     CDS_motif       7095..7160
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0012"
                     /evalue="4.10E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1201"
                     /protein_start="1179"
                     /score="25.8"
                     /tool="antismash"
                     /translation="MHHIISDGVSVHTLIREFGDLY"
     CDS_motif       7191..7223
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0013"
                     /evalue="2.90E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1222"
                     /protein_start="1211"
                     /score="13.5"
                     /tool="antismash"
                     /translation="QYKDYAVWQQE"
     CDS_motif       7482..7571
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0014"
                     /evalue="1.80E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1338"
                     /protein_start="1308"
                     /score="46.1"
                     /tool="antismash"
                     /translation="EIVVGSPIAGRPYADLESILGMFVNTLALR"
     CDS_motif       7620..7739
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0015"
                     /evalue="2.30E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1394"
                     /protein_start="1354"
                     /score="68.2"
                     /tool="antismash"
                     /translation="RQTALEAYEHQDYPFEELVDMLGVQRDMSRNPLFDTTLTL"
     aSDomain        8079..9281
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS20525_AMP-binding.2"
                     /evalue="2.50E-113"
                     /label="MOE42_RS20525_AMP-binding.2"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1908"
                     /protein_start="1507"
                     /score="370.7"
                     /specificity="substrate consensus: Gln"
                     /tool="antismash"
                     /translation="LEKQAAKTPERPAVFYGEEVISYKELNERANRVAWELIERGVTSE
                     TVIAITGRRSPEMLIGIYAILKAGGAYVPIDPEYPEERIRFLLKDSGAKLLLVESGSFE
                     IPSFEGDVLDLNINVSREKNHLINPDVSVRSNSLAYIIYTSGSTGTPKGVQIEHQSAVN
                     FLQSLQTRYPLNESDMILHKTSYSFDASIWELFWWPYAGASVFLLPQGGEKDPEMMIKV
                     IEEKRITAAHFVPSMLHAFLAYVKHRAVPIVPNGLKRVFAGGEQLGAHLVSRFYELLPG
                     VELTNSYGPTETTVEAAYFDCPLGKDLKRIPIGKPIHNLRLYILNKKKQILPTGCTGEL
                     YIAGNGVARGYLNRPELTTERFLSDPFYPGERMYKTGDLARWLPDGQVEFLGRLDDQVK
                     IR"
     CDS_motif       8268..8306
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0016"
                     /evalue="1.60E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1583"
                     /protein_start="1570"
                     /score="18.0"
                     /tool="antismash"
                     /translation="AILKAGGAYVPID"
     CDS_motif       8493..8552
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0017"
                     /evalue="1.80E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1665"
                     /protein_start="1645"
                     /score="32.6"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGTPKGVQIEH"
     CDS_motif       8937..8966
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0018"
                     /evalue="3.30E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1803"
                     /protein_start="1793"
                     /score="7.3"
                     /tool="antismash"
                     /translation="SYGPTETTVE"
     CDS_motif       9075..9164
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0019"
                     /evalue="4.00E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1869"
                     /protein_start="1839"
                     /score="54.3"
                     /tool="antismash"
                     /translation="PTGCTGELYIAGNGVARGYLNRPELTTERF"
     CDS_motif       9246..9311
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS20525_0020"
                     /evalue="1.20E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE42_RS20525"
                     /protein_end="1918"
                     /protein_start="1896"
                     /score="39.7"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
ORIGIN
        1 ttatcgggac acaagccgca atccgctgtt tgacgtcatg ttcgctcttc aaaacataga
       61 tcatgaaaat ctgatgctgg aagatttgcg tctccggtct gctgatattg agcataaagt
      121 gtccaaattc gatttgacac tttatgcatc ggaagaaccc aaaggggagc tccgttttca
      181 tctggaatat aatactgatt tatacaagaa aaaaacagta gaacaatggc taaaatactt
      241 gattcatatc ttccgggaaa tcaccgaaga tcattcagta actctaggga aaatcaatat
      301 agttgatgaa gatgagacaa gattaatcat tcacgaattc aaccgtacaa aaatggaata
      361 tccaaggcat gaaacagtca gccgcctttt tgaaagacaa gcggcaaaaa cgcctgatgc
      421 cgcagccgtt gtcagccatg gacaaacatt gacttatcat gaactgaaca atcgggcgaa
      481 caggatcgcg gcggtgcttc gggcaaaagg ggtcggtcct gaaagtgtcg tagctttatt
      541 gactaagcgc acaccagagc ttgcggccgg aatattagga attttgaaag caggaggggc
      601 ttatcttcct atcggagaag acacgccgtc agaacgaata gaatggatgc tgtcagatag
      661 cggggctgga attctccttc aatcagaaaa actcgggaac gatttcttaa atatagaagg
      721 agaatatgaa caaattttca tcgaagcgat tcaagagcaa ggaaatgata cggaaaacct
      781 tgaatcactt gccggaccgg attcacttgc ctatatcatc tatacatccg gatcaactgg
      841 gaaaccaaaa ggagtaatga ttgagcagcg aagtgtcatt cgtcttgtga caaacagcaa
      901 ttatattgag gttacacctt cagaccggct tctgatgact tcttcatttg gtttcgatgt
      961 aactactttt gaaatattcg ggccgctact taatggcgca gccctatata tggccgacaa
     1021 agaaacgttc cttgattccc atcagcttga gacatacata caccaaaacg gaatcacaac
     1081 attatggctg acttcacctc tttttaatca tttgaccgaa cagaatgaga atacgtttgc
     1141 cggtctgtcc acattgatca taggcggtga ggctttatct gccagccacg tcaatcggat
     1201 tcggaaagcc tgcccgtcac tggcaatatg gaatggatat gggccgactg aaaatacgac
     1261 gttctcaacc tgctttgaga ttgagagata ctacgagcat tcaataccga ttggccgtcc
     1321 gattggaaat tcaacagctt atatattaaa caactgggga atgcttcagc cgattggaac
     1381 agtcggagaa ttatgtgttg gcggtgatgg agtagcaaga ggctatcttg gccgtcctga
     1441 gttaacaaaa gaaaaatttg tgcctaaccc ttttgcaatt ggcgaacgaa tgtaccgaac
     1501 cggcgattta gcctgctggc tgccggatgg aactattgaa tatgccgggc gtatggacga
     1561 gcaggtgaaa atccgcggct accgggtcga gcttggtgaa atagaaacag cgattcggca
     1621 tatagacgga gtcaaggaag ctgccgtatt ggcgcgcccg ggacagtctg gaagcaaaga
     1681 gttagtcgca tacatgagtt tgcaagcaga catggatgct gaaaaagtac gttcacagct
     1741 ttccagcaca ttaccgggat atatgatacc tgtatatatg attgaaatgg acgctcttcc
     1801 gctgaccgca aacgggaaac tggacagaaa agcgctccct gaaccggaac tctcatcaaa
     1861 atcaaagtac gtacagccgc gaagtgagct tgaagaacag cttgcgctga tatggcagga
     1921 agtgcttggc cttgagaaaa tcggaattga agattcgttt tttgaactgg gcggagattc
     1981 aatcaaagct cttcaagtat ctgcacgtct tggtcgttac ggctggagta tgacggcaag
     2041 cgatttattc cggcacccga gaatcaaaga tgtatctgct gtaattcgca aaacagaaag
     2101 agtgattgac caagggccgg tgaagggcga agttccattg acacctgtcc agcactggtt
     2161 ccttactcaa cgtataacag acagacacca tttcaatcag tctgtgatgc tctttaactc
     2221 cggcagattg atagagaaac cgctgcggga gactttacaa aagcttgcgg aacaccatga
     2281 cgcacttcgt atgatatacc gcaaagagag cggacatgta atacaaatca atcaagatat
     2341 tcatgaaaca gaattgtaca ctttgaacgc ctcagatttt tcagatacag aagcttggga
     2401 aaatgaaatt gagaaagaag tcgcagctct tcaaagaggg atgaatatag aagaaggacc
     2461 gttgttaaga gcaggctggt ttaaaacgcc ttcgggagac tatttgttcc tgacaattca
     2521 ccatctagtc gttgacgggg tatcgtggcg gactttgctt gaagatttgt ctgccggcta
     2581 tcatcaagcg gcagctgggc aatccattca gcttccgccc aagacagatt catataagga
     2641 gtacgcagaa cgcctccaag attacgcgca aagcagcaag ctgattcaag aagaagctta
     2701 ttggcgttct gtagaagagg aaacaatagc ggcactccca tatgaaaagc cgcagcacaa
     2761 tgatacaaat gacagcagtc aaaacacagt gcgctttaca ctttctgaag aagacacgac
     2821 cgtcctgctt caaaaagtaa atcacgctta tggcacagat acacaagata ttctgcttac
     2881 ggcagcagca ttagcgattc gggactggac cggggaaagc aaacttcgga ttgcgatgga
     2941 gggacacgga agggaaaaca tactccctaa tgtggatatc agccgcacag tcgggtggtt
     3001 cacctcaatg tacccagtgc ttcttgagat tcaaaaagag tcaggcgagt tgggaactgc
     3061 ggtgaaaaca gtgaaagata cgcttgggcg aatacctcag aaagggaccg gttacggcat
     3121 tttaaaatat ataacaaagc cggagaacaa aagcatacag tttgggggag ctcccgagat
     3181 cggatttaac tacttagggc aattcaacga tacagagcag caagaaacct tcagcttatc
     3241 agggcttgca agcggggagg acatcacacg ctcatggcag cgtgagcaaa taatagagat
     3301 cagtgcattg gcggcggaaa acaaactgca ctttaatctc agctatccgc caaaccgatt
     3361 ttacagaaaa acgatggaac atcttttgaa cgggattcaa aactatttgc aaagcattat
     3421 gaaacattgc gcaggcaaac aggagacaga aaagacattg agcgatttca gcagtaaatc
     3481 gttgacaatg gaggatttgg acagcatttc cgacttagtt caagaactgt agaaaatcgt
     3541 gacagggaga catgaacatg acaaaagcga attccattca agatatttat ccgctgtctt
     3601 acatgcagga agggatgctt ttccattccc ttctgcatga agactcacgg gcatattttg
     3661 agcaagccgt attttcaatc gcaggagaag tacattcacg cttttttcag gacagcatca
     3721 atgccctagt ggcacgccat gacattttca gaaccatttt cattaaccaa aatgtttccg
     3781 caccgcagca agtggtactc agagatcgga ctgtaaccgt ccaagaagag gacatttctc
     3841 acttagacga atcccgtcaa tctgaattca tcgagaacta taaagaaaag gacagggaga
     3901 agggattcaa cctgcaaaaa gatgtgctta tgcgcatctc tctcctgaaa actgggaaac
     3961 agcaatatac ctgcctctgg agttttcatc acattgtgat ggatggctgg tgcttcagca
     4021 ttgtgctgaa agaatttttc caaatttacg ctgcccatat caatgcttca cctttaacac
     4081 tggagcccgt ccggccatac ggaagctaca tcaattggct gatggagcaa gacaaggagc
     4141 aggcgataac ctactggaaa agttatcttg ccggatatga gcagcaaact gcactgccaa
     4201 aacagaataa aacaaaggac aaacaaaaaa gcggacaagc gcatgtcacg ttttcgttct
     4261 caaaaaaaga cagcagcaga ctttcagaaa tggctgtaaa ggaagaggta acactcagta
     4321 cattgtttca tacgctgtgg ggcattctgc tgcaaaaata taataacacc gatgacgcgg
     4381 tatttggatc agtcacctcg ggaagacctt cttctattga agggattgaa aatatggtcg
     4441 gcctttttat caatacaatt ccagtacgtg tgcaaggagc tgatacgccg gtttccgagc
     4501 tgatgaaaag gatgcaaaaa gaagctctgg atgctgaagc gcacagttac catccgctat
     4561 acgagataca ggcacactct gccgtaaagc aaggtctgat tgatcatatt cttgtttttg
     4621 aaaactatcc tgtccagaat gaaatcgata tgctgaacag tcacgatgat gcccaagaac
     4681 tctttcagat tcatcatttt tcagtggcgg atgaaaccaa ttacagcttt tatctgatgg
     4741 tggcgccggg tgacgaaatc catatcaaaa tgagctatga ttccggtgta tacgaccgat
     4801 cgtttatatt aggggtcaaa ggacatctga ctcatatggt ttcgcaaatt ctagacaacc
     4861 cggatatctc tcccgccgga attgacatca caacgagcag cgagaaacat ctgctgctcg
     4921 gaaaaaagga agctgatgca cctgcttttc aaacgattca ttctatgttt gagaaacaag
     4981 cggaactgac gccgaatatg caggccgtca cctatcaagg ccggtcaata acatacagag
     5041 agctcaatga aaacgccaat aaactggctc ggatcttgcg aaagcgaggc gtaagaagac
     5101 aagaaccggt cgccatcatg gccgaacgct cacctgaatt aacggccggt atcctcggaa
     5161 tcttgaaagc aggcggctct ttggtcccga ttgacccaaa ttatccccct gaaaggatcc
     5221 ggtttattct tcaggatagc tgctgttcac atttggtatc acaccgaagc ctggcctctt
     5281 tgttggaatc ggtcaatgag gaagctttaa gaactgttac atatacggaa gaaagtgcgc
     5341 aggaagagga cggaagcaat gtgaagcccg tcaatacagc ggatgacttg ctgtatacca
     5401 tttatacatc gggcacaacc gggaaaccaa agggcgttca gcttgaacat aggagcatgg
     5461 caaatttgct gcactttcaa ttcacaaact ccggaattaa ttttcggact aacgtgctcc
     5521 aatatgccac acctgccttt gatgtctgtt atcaagaaat attttcaacg ctggctggag
     5581 gcggcacagt ccacatcgtg cctgaaccta tcaaaagaga cgtttctcag ctgtttgcgt
     5641 ttatcgccga ccataatgca gaaattgtat tctttccaac tgcgttcatt aaaatgatgt
     5701 tcagcgagga agaatacgcg aacgcatttc ctgatggcgt gaagcatctg attacggccg
     5761 gggaacaact aacgatctcc caactcttcc aacaagtact gcgccgtcac aacacggtgt
     5821 tacacaatca ttacggacct tctgaaaccc atgtcgtatc tgcttatagc attcatccgg
     5881 gggagcatat ccctgaaagt ccgccaatcg gcaaaccgat tgatcatacg catatgtata
     5941 ttttaaacgg acaaaaacag cttcaaccga agggcatcgc gggtgaattg tatatttccg
     6001 gttcaagcgt ggcaaggggg tacttccgca atgaagagct gactcgagaa aagtttttgc
     6061 cagatccttt cagacccgga gcaacgatgt ttcgcacagg cgacatggca agactgtcag
     6121 aggacggcaa tattgaatat atcggccgga ccgatgatca ggtgaaaatc cggggttacc
     6181 gggtcgaacc tggagaaatt gagatgacat tacagaatca cccgaaaatt aaggaagcgg
     6241 cggttatcat taggcaagat catgctggag aaaatgaatt gtgtgcttac tacagcgcgc
     6301 ctaaccgatt gaattctcta gctttgcgcc gctatcttgc agaagaaatg ccggaatata
     6361 tgcttccgtt aaagtgggga tgggtggaaa gcataccgct gactccgaat ggaaaagtcg
     6421 acaaaagatc tcttccagaa ccggaagctg ccgcagatga taagcaatat gccgcaccga
     6481 gaaatcttac ggagctgaag ctggcacaac tttgggaaga cgtgctgaaa gacggcccgg
     6541 tgggaatccg ggataacttt ttcgaccggg gaggccactc attgaaagca acagcgcttg
     6601 tctcccgtat tgctaaggag gtcggcgtta aggtgccgct tcaagaagtg tttgcccatc
     6661 cgacggtgga gggtctggct gctgtcgtcc gggaagcaga ggaaaacccg tatgcggcaa
     6721 ttgagccggc accagaacaa gatgtttatc ctgtctcatc agcgcaaaag agaatgtatg
     6781 tgcttcagca gatggaagac ggcggcgtcg gctacaacat gccggcggta ctggaactgg
     6841 aaggaaagct agaccgaggg cgtttggaaa ctgtattcaa aaaactgatc agccgccacg
     6901 aaccgcttcg aacctccttt gaagaaggaa cagacggcga accggtccag cgcattcatt
     6961 caaagataac atttgctcta tgtgaacaag aaaagacatc ggcatttatc cgaccttttg
     7021 atctcagcaa agccccgctt ttacgggcag gcctaatcaa ggaaacagac gaacgccatc
     7081 tgctcttggt cgatatgcat cacatcattt cagacggtgt gtccgttcat acgttgattc
     7141 gagaattcgg cgatttgtat gcacaccgaa cgcttgaacc cttacgtatt caatataaag
     7201 actatgctgt gtggcagcag gaattcaaaa gtggcggcac gtatcaaaaa caggaagctt
     7261 actggctgaa tcagcttagc ggtgagctgc ctgtgcttga actgccgaca gacaagcttc
     7321 ggccggcagt gcgaaccttc gccggagaca aagtttcatt catcatcagc aaagagatca
     7381 catccggtct gaaacaactt gcacgcaaaa acgaatgtac tctgtacatg acgctccttg
     7441 ctgcatatac agcattcttg gcgcggctga gcggacagga agaaatagta gtcggatcgc
     7501 ctattgccgg acggccgtat gccgatcttg aatctatact cggcatgttc gtcaacacac
     7561 tggcgctccg gacacaccct gaaggcggta agcgtttttc agaatacttg caggaagtgc
     7621 gacagacagc gttggaagcc tatgagcatc aggattatcc gttcgaagag ctggttgaca
     7681 tgctcggtgt ccagcgggat atgagccgca acccgttatt tgacacgacg cttacgctcc
     7741 aaaacatgga acagcaaaag ctgcaattag acggtttgca acttaaaacc gcagatgttg
     7801 accacccaat atctaaattt gatatatctc tttatgtaat ggagaacgag gaagagcttt
     7861 attgtcaatt tgaatacagc acttcattgt ttgaaaagga aacgattcaa cgctggtcgc
     7921 gatttttctt gagactggta gaaaatactg ttagcagccc tgacacagaa ttagacgaca
     7981 tctcggtgct tacaaaagaa gaagagaaga cctttattca atcattcagt ccgcttccga
     8041 caacaagctt tccgctggct cagacactgc atcaccagct tgaaaaacaa gcggcaaaaa
     8101 caccagaacg gccggctgtc ttttacggag aggaggttat ctcctacaaa gaactgaacg
     8161 aacgggccaa ccgagtcgct tgggagctga ttgaaagggg agttacatca gaaactgtca
     8221 ttgcgatcac aggcaggcgg tcaccggaaa tgctgattgg catatatgcc attttgaaag
     8281 cgggcggcgc ttatgttcct atcgatccgg agtacccgga agaacgtatc cgctttttac
     8341 tgaaggacag cggtgctaag cttcttttag ttgaatccgg cagtttcgag atcccgtctt
     8401 ttgaaggtga cgtactagat ctcaacataa atgtcagccg agaaaagaat catcttatca
     8461 atcccgatgt gtcagtacgg tcgaattcgt tagcctatat catctataca tcaggctcaa
     8521 ccggaacgcc aaaaggtgtg cagatcgagc atcagtcggc agtgaatttt ttgcaatccc
     8581 tgcaaacccg ctatccgctt aacgaatcgg atatgatttt gcataagaca tcttattcgt
     8641 ttgacgcttc catatgggag ttgttctggt ggccgtacgc aggtgcttcc gtttttctgc
     8701 ttcctcaagg aggcgaaaag gatcccgaaa tgatgataaa ggtgatagaa gaaaaacgaa
     8761 taacggcagc tcattttgta ccctctatgc tgcatgcatt tttagcatat gtgaaacatc
     8821 gcgctgtacc gatcgtacct aacggcctga agcgagtatt tgccggcggt gagcaactgg
     8881 gcgcacatct tgtttcccgt ttttatgaac tgcttcccgg tgtagaattg accaattctt
     8941 atgggccgac agaaacgaca gtagaagcag catactttga ttgtccgtta ggtaaagacc
     9001 tgaagcgaat tccaatcggt aaacctattc acaatctccg gctgtatatt ttaaacaaga
     9061 agaagcagat tctgccgacc ggttgtacag gcgaactgta tatcgccggg aatggggtgg
     9121 ccagaggcta cctgaatcgc cccgagttaa cgacggaacg ctttctctct gatccctttt
     9181 atcctgggga acggatgtac aaaacgggag atctggcgcg ctggctgccg gacggacagg
     9241 ttgagttcct cggccgtttg gacgatcaag tcaaaatccg cggctaccgg atcgaacccg
     9301 gcgaaattga atcagctctc cggaacttgg aaggcgtgca agaagcagcc gttatcaccc
     9361 gcgcagacag cggggaggca gagctatgcg cttatatcca ggggcgtgat aaaaacgacg
     9421 tgcgggcaca gcttgcgctc gtgctgcccg gctatatgat ccccgctcat tttgtgcaaa
     9481 ttgacacatg gccaatcaca ccaagcggga agcttgacca gaaggctttg cccgctcctg
     9541 atggagcggt tggtcaagaa tcatacaagg caccaagaaa tcttaccgaa atgaagctgg
     9601 cccagctttg ggaatacgnn cggtatcacg gacgactttt tcgaccgggg aggccactcg
     9661 ttaaaagcaa cggcgctcat ctcccgaatc gccaagacat tcggcgtcca agtgccgctt
     9721 aaagacgtat ttgcccgacc gaccatagaa gggctggctt ctgtcatccg cgagttggag
     9781 gaaagcccgt atgcgacgat cgaaccgacc gaaaagcggg acacctatcc ggtctcctcg
     9841 gcgcaaaaac ggatgtatgt ccttcagcag ctggaagatg gaggcaccgg ctacaatatg
     9901 cctgcggtgc ttgagctgga aggcaagctt gaccgtaaac gaatggaggc agctttccaa
     9961 gcgctgatca gccgccatga gtcgctccgg acgtcctttg aaaccggaaa cggaggggag
    10021 ccggttcagc atatccatca tgaggtgccg tttacggtga ccgaagagaa ctcggctgat
    10081 gcgtttgtac gtccctttga tctcagccag gctccgcttt tcagagcggg actcgtaaaa
    10141 gaaacaaacg agcggcatgt ccttttggtc gacatgcatc atatcatttc tgacggcgtc
    10201 tcagtcaata cgctcattca ggaatttgga gaattgtacg ccgggcggaa acttgagccg
    10261 ttgcgcatcc aatacaaaga ttacgccgtg tggcagcagg aattcaaaaa aggcgacaca
    10321 tatgagaagc aggaatctta ttggctgaag cagctcggcg gcgagctgcc tgtgcttgaa
    10381 ctgccggcgg acaagccccg tccggcggtg cgaagcttcg ccggtgacaa gatctcattt
    10441 ac
//
