LOCUS       NZ_JALAUL010000010      2605 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus atrophaeus strain CK4F1-2 Contig_0000010, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAUL010000010
VERSION     NZ_JALAUL010000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 219.01779805621194x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:32:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,147
            CDSs (total)                      :: 4,069
            Genes (coding)                    :: 3,904
            CDSs (with protein)               :: 3,904
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 97 of 165
            Pseudo Genes (incomplete)         :: 106 of 165
            Pseudo Genes (internal stop)      :: 39 of 165
            Pseudo Genes (multiple problems)  :: 66 of 165
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:35:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2605
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAUL010000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..2603)
                     /NRPS_PKS="Domain: AMP-binding (128-521). E-value:
                     2.8e-116. Score: 380.4. Matches aSDomain:
                     nrpspksdomains_MOE42_RS18790_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (628-695). E-value: 5.9e-26. Score:
                     82.5. Matches aSDomain: nrpspksdomains_MOE42_RS18790_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (705-867). E-value:
                     6.9e-69. Score: 224.3. Matches aSDomain:
                     nrpspksdomains_MOE42_RS18790_Epimerization.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390539.1"
                     /locus_tag="MOE42_RS18790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE42_18735"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268486930.1"
                     /sec_met_domain="AMP-binding (E-value: 3.5e-123, bitscore:
                     397.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.1e-18, bitscore:
                     53.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.4e-37, bitscore:
                     115.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRDTSRNPLFDAMLVVQNIEHEQLTMDGLQLKPAGASHSVSKFDL
                     TLQASEGNKEIHLQFEYATALFQKKTIERWASHLSNMLHEIAHSPQTVLRDISILTQKE
                     KDLLLNGFNNVTETHVQDQTLQELFQTQAAKTPEATALVSGEKTLTYQELDQWSNRIAR
                     TLRTKGVGPDTAVGVLAPRSFSMTAAILGIWKAGGAYVPLDPEYPAERKNYILSDSRAK
                     LLVTVSAAAHKAPADYEGEVLLLEDVCENDASRLPAYSYSHNLAYIIYTSGTTGQPKGV
                     MVEHRGIANTLEWRRNAYALHEEDTVLQLFSFAFDGFVTSMFTPLLSGAKVVLPDEEEA
                     KDILVIKHNLAYYRITHMIIVPVLYRALLDVLKPEDVQTLRIVTLAGEAADRELTDRSL
                     AISPNTELANEYGPTENSVATTAMRHLERQKTISIGKPINNTQVYILDGNNLQPIGVAG
                     ELCIAGTGLARGYVNLPELTEQTFVPHPFHSGKRMYRTGDMAKWQADGTLEYIGRMDDQ
                     IKIRGHRVEIEEVETVLRRTDGVKEAAVLARETPLGQMELCAYMVTEPETSVESVRAAL
                     ASSMPAYLIPAFLLKLDALPLSPNGKLDRRALPQPVHEGQTQRSFQAPETAMEKELANI
                     WTEVLGAERIGTDESFFELGGDSIKALQVSARLHRYGKQMSIKDLFRYPTIKELACYIR
                     ASKENASQSPVEGEVQWSPVQKWFLSQDLEEKHHFNQSVMLYRETPVDEETLRKTLKAI
                     VEHHDALRLIFPKNGRENWSQFNLPIGASEEALYGLQIIELKAPVDGSPTEPAWYEAAI
                     KNHVAKIQHGMDLEKGPLFQAGLFRTAGGDFLFLAAHHLTVDGVSWRVILEDLASG"
     source          1..2605
                     /db_xref="taxon:1452"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus atrophaeus"
                     /strain="CK4F1-2"
                     /submitter_seqid="Contig_0000010"
     gene            complement(<1..>2605)
                     /locus_tag="MOE42_RS18790"
                     /old_locus_tag="MOE42_18735"
     region          1..2605
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2605
                     /SMILES="NC(Cc1ccc(O)cc1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2605
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2603](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2603)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        complement(2..487)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS18790_Epimerization.1"
                     /evalue="6.90E-69"
                     /label="MOE42_RS18790_Epimerization.1"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="867"
                     /protein_start="705"
                     /score="224.3"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLTVDG"
                     /tool="antismash"
                     /translation="EGEVQWSPVQKWFLSQDLEEKHHFNQSVMLYRETPVDEETLRKTL
                     KAIVEHHDALRLIFPKNGRENWSQFNLPIGASEEALYGLQIIELKAPVDGSPTEPAWYE
                     AAIKNHVAKIQHGMDLEKGPLFQAGLFRTAGGDFLFLAAHHLTVDGVSWRVILEDLAS"
     aSModule        complement(2..2218)
                     /complete
                     /domains="nrpspksdomains_MOE42_RS18790_AMP-binding.1"
                     /domains="nrpspksdomains_MOE42_RS18790_PCP.1"
                     /domains="nrpspksdomains_MOE42_RS18790_Epimerization.1"
                     /locus_tags="MOE42_RS18790"
                     /monomer_pairings="Tyr -> D-Tyr"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(8..73)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS18790_0006"
                     /evalue="4.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="865"
                     /protein_start="843"
                     /score="38.1"
                     /tool="antismash"
                     /translation="LFLAAHHLTVDGVSWRVILEDL"
     CDS_motif       complement(308..385)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS18790_0005"
                     /evalue="5.70E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="765"
                     /protein_start="739"
                     /score="18.8"
                     /tool="antismash"
                     /translation="PVDEETLRKTLKAIVEHHDALRLIFP"
     aSDomain        complement(518..718)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS18790_PCP.1"
                     /evalue="5.90E-26"
                     /label="MOE42_RS18790_PCP.1"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="695"
                     /protein_start="628"
                     /score="82.5"
                     /tool="antismash"
                     /translation="EKELANIWTEVLGAERIGTDESFFELGGDSIKALQVSARLHRYGK
                     QMSIKDLFRYPTIKELACYIRA"
     CDS_motif       complement(1010..1075)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS18790_0004"
                     /evalue="2.00E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="531"
                     /protein_start="509"
                     /score="32.7"
                     /tool="antismash"
                     /translation="YIGRMDDQIKIRGHRVEIEEVE"
     aSDomain        complement(1040..2218)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE42_RS18790_AMP-binding.1"
                     /evalue="2.80E-116"
                     /label="MOE42_RS18790_AMP-binding.1"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="521"
                     /protein_start="128"
                     /score="380.4"
                     /specificity="substrate consensus: Tyr"
                     /tool="antismash"
                     /translation="FQTQAAKTPEATALVSGEKTLTYQELDQWSNRIARTLRTKGVGPD
                     TAVGVLAPRSFSMTAAILGIWKAGGAYVPLDPEYPAERKNYILSDSRAKLLVTVSAAAH
                     KAPADYEGEVLLLEDVCENDASRLPAYSYSHNLAYIIYTSGTTGQPKGVMVEHRGIANT
                     LEWRRNAYALHEEDTVLQLFSFAFDGFVTSMFTPLLSGAKVVLPDEEEAKDILVIKHNL
                     AYYRITHMIIVPVLYRALLDVLKPEDVQTLRIVTLAGEAADRELTDRSLAISPNTELAN
                     EYGPTENSVATTAMRHLERQKTISIGKPINNTQVYILDGNNLQPIGVAGELCIAGTGLA
                     RGYVNLPELTEQTFVPHPFHSGKRMYRTGDMAKWQADGTLEYIGRMDDQIKIR"
     CDS_motif       complement(1157..1246)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS18790_0003"
                     /evalue="2.10E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="482"
                     /protein_start="452"
                     /score="48.8"
                     /tool="antismash"
                     /translation="PIGVAGELCIAGTGLARGYVNLPELTEQTF"
     CDS_motif       complement(1748..1807)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS18790_0002"
                     /evalue="2.40E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="285"
                     /protein_start="265"
                     /score="35.3"
                     /tool="antismash"
                     /translation="AYIIYTSGTTGQPKGVMVEH"
     CDS_motif       complement(1991..2032)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE42_RS18790_0001"
                     /evalue="2.00E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOE42_RS18790"
                     /protein_end="204"
                     /protein_start="190"
                     /score="20.7"
                     /tool="antismash"
                     /translation="LGIWKAGGAYVPLD"
ORIGIN
        1 cccggacgcc agatcttcta aaatcacacg ccatgaaaca ccgtccactg tcaggtgatg
       61 agctgcaaga aacaggaagt caccgcctgc agtacggaat aaccccgctt ggaacaacgg
      121 ccctttctct aggtccatcc cgtgctgaat tttcgccaca tgatttttaa tagccgcttc
      181 gtaccatgcg ggttcagtcg gtgatccgtc taccggtgcc tttaattcaa tgatctgaag
      241 tccgtacaat gcttcctcag acgcaccgat aggcaaattg aactgactcc agttttcacg
      301 tccgtttttc gggaaaatta accgtaatgc atcatgatgt tcgacaatgg ctttcaaggt
      361 tttacggagc gtttcttcat ccactggagt ctcccggtac agcatgactg actggttgaa
      421 atggtgtttt tcctcaaggt cttgagacag aaaccacttc tgaacaggag accactggac
      481 ctctccctcc acggggcttt ggctggcatt ttccttggat gcccggatat agcatgccag
      541 ttctttaatt gtcggataac ggaacaaatc tttaatggac atttgttttc cgtaccgatg
      601 cagcctcgcc gacacctgta atgctttgat tgaatctcct ccgagctcga aaaacgattc
      661 atccgttccg attctttctg cacccagcac ttctgtccag atgttagcca attctttttc
      721 catagctgtt tctggagctt ggaagctgcg ctgggtttgt ccttcatgta caggctgcgg
      781 aagtgccctc cgatccagtt ttccatttgg cgacaaggga agagcgtcca gttttaacag
      841 gaaggcaggg atcaaataag ccggcatgct gcttgccaat gcggcacgca cgctttcaac
      901 acttgtttct ggctctgtga ccatatatgc gcacagttcc atctggccga gaggcgtttc
      961 acgtgctaag actgccgctt cttttactcc gtctgtgcgg cgcaggacgg tttcaacttc
     1021 ttctatttcc actctgtgcc ctctgatttt aatctggtca tccatccgtc cgatatattc
     1081 aagcgtgccg tctgcctgcc acttcgccat atcccctgtt cgatacattc gttttccaga
     1141 atgaaaagga tgcggaacaa aagtttgttc ggtcagttcg ggaaggttga catatcctct
     1201 ggcaagtccg gttcctgcaa tgcacaattc cccggcgaca ccgatcggct gcaaattgtt
     1261 tccatctaaa atatagactt gtgtattgtt tatcggcttt ccgatactta tcgttttctg
     1321 ccgttctaaa tgtctcatcg ccgttgtagc tacactgttt tctgtcggtc catattcatt
     1381 ggcaagctct gtattcggtg aaatggccaa acttcgatcg gttaattccc tgtctgcggc
     1441 ttcgcctgcc aatgttacga ttctaagcgt ctgaacatct tcaggtttca atacatctaa
     1501 aagcgcccta tacagaaccg gaacgataat catatgggtg atgcgataat acgcaagatt
     1561 atgcttaatg acgagtatat ctttggcttc ttcctcatcg ggcagcacga ctttcgctcc
     1621 tgataaaagc ggtgtgaaca tgctggtcac aaaaccgtca aatgcaaagg aaaagagctg
     1681 aagcactgta tcttcttcat gtaaagcgta agcattcctg cgccattcga gggtattggc
     1741 tattcctctg tgttctacca ttaccccttt aggctgtcct gttgttcctg atgtgtaaat
     1801 gatatatgcc aggttgtgag aatatgaata tgcaggcaga cgggaagcat cattttcaca
     1861 cacgtcttca agcagcagaa cttcaccttc ataatctgct ggtgctttat gggccgcagc
     1921 actgactgtg actagcagtt ttgcgcggct gtcactcaag atataattct tacgctccgc
     1981 aggatactcc ggatcaagag gaacataagc gccgccagct ttccagattc ccagaatagc
     2041 cgctgtcatg ctgaatgaac gcggcgccaa tacacctacc gctgtgtccg ggcctacacc
     2101 cttagtgcgt aaagtccggg cgattctgtt ggaccactga tccagctctt gataagtaag
     2161 tgttttttca ccgctgacaa gagctgttgc ttctggtgtt tttgcggctt gtgtttgaaa
     2221 aagttcttga agcgtttggt cttgaacgtg cgtttcagtt acgttattaa atccattcag
     2281 cagcaggtct ttctccttct gggtaaggat gctgatgtcc ctcaaaactg tctgcgggct
     2341 gtgagcgatc tcgtgcagca tgttcgataa atgagatgcc cagcgctcta tcgttttctt
     2401 ttgaaacagt gctgtcgcat attcaaattg cagatgaatt tctttgtttc cttcagaagc
     2461 ttgcagcgtt aaatcgaatt tagatacaga gtgggaagca ccagccggtt tcagctgaag
     2521 tccatccatt gtcagctgct catgctcgat attctgcaca acgagcattg catcaaacag
     2581 cgggttgcgg cttgtgtccc gataa
//
