LOCUS       NZ_JALAUJ010000017      1835 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus atrophaeus strain CK4F1-4 Contig_0000017, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAUJ010000017
VERSION     NZ_JALAUJ010000017.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 412.8916062413295x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:32:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,185
            CDSs (total)                      :: 4,107
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 83 of 146
            Pseudo Genes (incomplete)         :: 103 of 146
            Pseudo Genes (internal stop)      :: 25 of 146
            Pseudo Genes (multiple problems)  :: 59 of 146
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:43:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1835
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAUJ010000017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1835
                     /db_xref="taxon:1452"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus atrophaeus"
                     /strain="CK4F1-4"
                     /submitter_seqid="Contig_0000017"
     gene            complement(<1..>1835)
                     /locus_tag="MOE37_RS20925"
                     /old_locus_tag="MOE37_20870"
     region          1..1835
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1835
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1835
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1835](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1835)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..1835)
                     /NRPS_PKS="Domain: AMP-binding (0-364). E-value: 3.5e-98.
                     Score: 320.8. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20925_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (469-537). E-value: 8.6e-28. Score:
                     88.4. Matches aSDomain: nrpspksdomains_MOE37_RS20925_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390754.1"
                     /locus_tag="MOE37_RS20925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE37_20870"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268533755.1"
                     /sec_met_domain="AMP-binding (E-value: 3.7e-116, bitscore:
                     374.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.2e-20, bitscore:
                     58.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 7.4e-21, bitscore:
                     60.5, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAKANRLARILIDCGVSADDRVGILTKPSLEMSAAVLGVLKAGA
                     AFVPIDPEYPDQRIQYILEDSGAKLLFTQEELSVPESYTGEVLLLNGERSILSLPLDEN
                     DRANPQTETNADHLAYMIYTSGTTGQPKGVMVEHHALVNLCFWHHDAFGMTAEDRSAKY
                     AGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIIRLNEYFEQNGVTITFLPTQLAEQFM
                     ELENTSLRVLLTGGDKLKRAVKQPYTLVNNYGPTENTVVATSTEIDPEEGSLSIGRAIA
                     NTRAYILGDGDQVQPEGVAGELCVAGRGLARGYLNREDETAKRFVADPFVPGERMYRTG
                     DLVRWTTEHGIEYIGRIDQQVKVRGYRIELSEIEVRLAQLSAVQDAAVAAVKDKAGNTA
                     LCAYVTPEDADTESLKSALKDTLPDYMIPAYWVTMDELPVTANGKVDRKALPQPDIEAQ
                     STAYKAPATEMEELLADIWQDVLGIPDIGISDNFFTLGGDSIKGIQMASRLNQHGLKLE
                     MKDLFQHPTIEELVSYVERTEGKEADQGPVEGEAILTPIQRWFFDRKFTNQHHWNQSVM
                     LHAPKGFDPIAVEKTLQALTEHHDALRMVYREEQG"
     CDS_motif       complement(15..77)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20925_0008"
                     /evalue="9.50E-04"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="607"
                     /protein_start="586"
                     /score="11.6"
                     /tool="antismash"
                     /translation="AVEKTLQALTEHHDALRMVYR"
     CDS_motif       complement(150..179)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20925_0007"
                     /evalue="7.90E-05"
                     /label="NRPS-E1"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="562"
                     /protein_start="552"
                     /score="15.4"
                     /tool="antismash"
                     /translation="LTPIQRWFFD"
     aSDomain        complement(225..428)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20925_PCP.1"
                     /evalue="8.60E-28"
                     /label="MOE37_RS20925_PCP.1"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="537"
                     /protein_start="469"
                     /score="88.4"
                     /tool="antismash"
                     /translation="EELLADIWQDVLGIPDIGISDNFFTLGGDSIKGIQMASRLNQHGL
                     KLEMKDLFQHPTIEELVSYVERT"
     aSModule        complement(225..1835)
                     /complete
                     /domains="nrpspksdomains_MOE37_RS20925_AMP-binding.1"
                     /domains="nrpspksdomains_MOE37_RS20925_PCP.1"
                     /locus_tags="MOE37_RS20925"
                     /monomer_pairings="Leu -> Leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(714..779)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20925_0006"
                     /evalue="1.20E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="374"
                     /protein_start="352"
                     /score="36.5"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
     aSDomain        complement(744..1835)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20925_AMP-binding.1"
                     /evalue="3.50E-98"
                     /label="MOE37_RS20925_AMP-binding.1"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="364"
                     /protein_start="0"
                     /score="320.8"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="MNAKANRLARILIDCGVSADDRVGILTKPSLEMSAAVLGVLKAGA
                     AFVPIDPEYPDQRIQYILEDSGAKLLFTQEELSVPESYTGEVLLLNGERSILSLPLDEN
                     DRANPQTETNADHLAYMIYTSGTTGQPKGVMVEHHALVNLCFWHHDAFGMTAEDRSAKY
                     AGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIIRLNEYFEQNGVTITFLPTQLAEQFM
                     ELENTSLRVLLTGGDKLKRAVKQPYTLVNNYGPTENTVVATSTEIDPEEGSLSIGRAIA
                     NTRAYILGDGDQVQPEGVAGELCVAGRGLARGYLNREDETAKRFVADPFVPGERMYRTG
                     DLVRWTTEHGIEYIGRIDQQVKVR"
     CDS_motif       complement(861..950)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20925_0005"
                     /evalue="2.30E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="325"
                     /protein_start="295"
                     /score="51.8"
                     /tool="antismash"
                     /translation="PEGVAGELCVAGRGLARGYLNREDETAKRF"
     CDS_motif       complement(957..986)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20925_0004"
                     /evalue="1.00E+01"
                     /label="Cy2"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="293"
                     /protein_start="283"
                     /score="-0.7"
                     /tool="antismash"
                     /translation="RAYILGDGDQ"
     CDS_motif       complement(1053..1082)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20925_0003"
                     /evalue="3.20E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="261"
                     /protein_start="251"
                     /score="10.1"
                     /tool="antismash"
                     /translation="NYGPTENTVV"
     CDS_motif       complement(1422..1481)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20925_0002"
                     /evalue="4.10E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="138"
                     /protein_start="118"
                     /score="34.6"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMVEH"
     CDS_motif       complement(1683..1724)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20925_0001"
                     /evalue="5.40E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOE37_RS20925"
                     /protein_end="51"
                     /protein_start="37"
                     /score="19.4"
                     /tool="antismash"
                     /translation="LGVLKAGAAFVPID"
ORIGIN
        1 tctccctgtt cttcgcgata gaccatacga agcgcgtcat gatgttccgt cagcgcctgt
       61 aacgttttct caaccgctat cgggtcaaag cctttcggtg cgtgaagcat caccgattgg
      121 ttccaatggt gctgattcgt aaacttgcgg tcaaagaacc agcgctggat cggcgtcaaa
      181 atcgcttctc cttctacagg accctggtca gcctctttgc cttccgtgcg ttctacgtaa
      241 gacaccagtt cctcaatcgt cggatgctgg aagagatcct tcatttccag cttcaagccg
      301 tgctggttca ggcggctcgc catttgaatt cctttgatgg aatcgccgcc aagtgtgaag
      361 aagttgtcgc ttattccgat gtccgggatg ccgaggacat cctgccaaat atcggctaac
      421 agctcttcca tttccgttgc cggcgccttg tatgccgtgc tttgcgcttc aatatcaggc
      481 tgaggcaggg ctttgcggtc aacctttccg ttggcggtca caggaagctc gtccatcgtt
      541 acccagtacg ccgggatcat gtagtccggc agtgtgtctt ttaacgcgga cttgagtgat
      601 tcagtatcag cgtcttccgg tgtgacatat gcacataacg cggtgttgcc cgctttatcc
      661 ttcaccgcag ccactgccgc gtcttgcaca gccgaaagct gggcgaggcg aacctcaatt
      721 tctgacagct cgatccggta gccgcgcacc ttgacctgct ggtcgattcg gccgatgtac
      781 tcgatgccgt gttctgtcgt ccatctgaca aggtcgcccg tccggtacat gcgttcaccc
      841 ggcacaaacg gatcggcgac aaaccgcttc gctgtttcgt cttcgcggtt cagatagccg
      901 cgcgcaagcc cgcggcccgc aacgcaaagc tctccggcca ccccttcggg ctggacctga
      961 tcgccgtcgc cgagaatata ggctctcgta ttggcaatcg cgcgtccgat ggaaagcgaa
     1021 ccttcctcag gatcgatttc tgtacttgtg gcaacgaccg tgttttcagt cgggccgtag
     1081 ttattgacga gcgtgtatgg ctgtttcacc gcccgtttca gcttgtctcc ccctgtcaga
     1141 agcactcgga gcgatgtgtt ttcaagctcc ataaactgct ccgccagctg ggtcggcagg
     1201 aaggtgatcg tgacgccgtt ttgttcgaaa tactcattca ggcggatgat atcgagacgg
     1261 atcgcttcat caatcacgtg aagctcggca ccgatcgtcc atgtcgggaa catttcccaa
     1321 atggatgcgt caaatccgaa gcccgcgtat ttcgcactgc ggtcctctgc cgtcatacca
     1381 aatgcgtcgt gatgccagaa gcacaggttc acaagcgcgt ggtgttcgac catgacaccc
     1441 ttcggctgtc cagtcgttcc tgacgtgtaa atcatgtagg cgaggtgatc cgcgtttgtc
     1501 tctgtctgcg gattcgcccg gtcgttctcg tcaagcggca ggctcaaaat ggagcgctcc
     1561 ccattcagaa gaaggacttc tcccgtgtag ctttcaggca cactgagttc ttcctgtgtg
     1621 aacagaagct tcgcgccgct gtcttctaaa atgtattgaa tccgctgatc cggatactca
     1681 gggtcaatcg gcacaaatgc cgcgcctgct ttcagcacgc ccagcaccgc ggcggacatt
     1741 tcgagcgacg gcttcgtgag aatgccgacg cggtcatccg cgctgacgcc gcagtcgatc
     1801 agaatacgcg ccagacggtt cgctttggcg ttcag
//
