LOCUS       NZ_JALAUJ010000015      8996 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus atrophaeus strain CK4F1-4 Contig_0000015, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAUJ010000015
VERSION     NZ_JALAUJ010000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8996)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 412.8916062413295x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:32:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,185
            CDSs (total)                      :: 4,107
            Genes (coding)                    :: 3,961
            CDSs (with protein)               :: 3,961
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 83 of 146
            Pseudo Genes (incomplete)         :: 103 of 146
            Pseudo Genes (internal stop)      :: 25 of 146
            Pseudo Genes (multiple problems)  :: 59 of 146
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:43:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8996
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAUJ010000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..1228
                     /locus_tag="MOE37_RS20860"
                     /old_locus_tag="MOE37_20805"
     source          1..8996
                     /db_xref="taxon:1452"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus atrophaeus"
                     /strain="CK4F1-4"
                     /submitter_seqid="Contig_0000015"
     region          1..8996
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..8996
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8996
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1250:>8996](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1251..>8996
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             <3..1228
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (0-245). E-value:
                     8.4e-127. Score: 415.8. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20860_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (250-404). E-value: 4.4e-55.
                     Score: 177.7. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20860_TIGR01720.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="MOE37_RS20860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE37_20805"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268496134.1"
                     /sec_met_domain="Condensation (E-value: 1.1e-69, bitscore:
                     223.2, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YREEQGDVIQYNRGLNEASVDMKVFKLNGPAIDNEEKIEREADHL
                     QSSIVLETGNLLKAGLFQAEDGDHLLLAVHHLAVDGVSWRILLEDFAAVYTQLKQGNKP
                     VLPQKTHSFAEYAERLKEFANTKAFLKETDYWRQLEENTIQTPLPKDRQSSDQRMKHTR
                     TVQFSLTAEETEKLTTKVHEAYHTEMNDILLTALGLAMKNWTGQDQVSVHLEGHGREEI
                     LEDISISRTVGWFTSMYPMVLNMKHADDLAYQLKQMKEDIRHVPNKGVGYGILRYLTAP
                     EHKTELEFSIQPEISFNYLGQFNEMADSGLFTRSSMPSGQSLSPETEKPNALDIVGYIE
                     NGTLTMTLAYHSLEFHERTIQAFSDSFKTHLLKVMEHCLAQDGTVLTPSDLGDDDLTLD
                     ELDKLMEIF"
     aSDomain        5..739
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20860_Epimerization.1"
                     /evalue="8.40E-127"
                     /label="MOE37_RS20860_Epimerization.1"
                     /locus_tag="MOE37_RS20860"
                     /protein_end="245"
                     /protein_start="0"
                     /score="415.8"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLAVDG"
                     /tool="antismash"
                     /translation="YREEQGDVIQYNRGLNEASVDMKVFKLNGPAIDNEEKIEREADHL
                     QSSIVLETGNLLKAGLFQAEDGDHLLLAVHHLAVDGVSWRILLEDFAAVYTQLKQGNKP
                     VLPQKTHSFAEYAERLKEFANTKAFLKETDYWRQLEENTIQTPLPKDRQSSDQRMKHTR
                     TVQFSLTAEETEKLTTKVHEAYHTEMNDILLTALGLAMKNWTGQDQVSVHLEGHGREEI
                     LEDISISRTVGWFTSMYPMVLNM"
     aSModule        5..1216
                     /domains="nrpspksdomains_MOE37_RS20860_Epimerization.1"
                     /domains="nrpspksdomains_MOE37_RS20860_TIGR01720.1"
                     /incomplete
                     /locus_tags="MOE37_RS20860"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       212..277
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20860_0001"
                     /evalue="2.50E-12"
                     /label="NRPS-E2"
                     /locus_tag="MOE37_RS20860"
                     /protein_end="91"
                     /protein_start="69"
                     /score="38.8"
                     /tool="antismash"
                     /translation="LLLAVHHLAVDGVSWRILLEDF"
     CDS_motif       686..733
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20860_0002"
                     /evalue="5.40E-09"
                     /label="NRPS-E5"
                     /locus_tag="MOE37_RS20860"
                     /protein_end="243"
                     /protein_start="227"
                     /score="28.0"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     aSDomain        755..1216
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20860_TIGR01720.1"
                     /evalue="4.40E-55"
                     /label="MOE37_RS20860_TIGR01720.1"
                     /locus_tag="MOE37_RS20860"
                     /protein_end="404"
                     /protein_start="250"
                     /score="177.7"
                     /tool="antismash"
                     /translation="LAYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKTELEFSIQPEI
                     SFNYLGQFNEMADSGLFTRSSMPSGQSLSPETEKPNALDIVGYIENGTLTMTLAYHSLE
                     FHERTIQAFSDSFKTHLLKVMEHCLAQDGTVLTPSDLGDDDLTLDELDKL"
     CDS_motif       779..838
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20860_0003"
                     /evalue="6.60E-10"
                     /label="NRPS-E6"
                     /locus_tag="MOE37_RS20860"
                     /protein_end="278"
                     /protein_start="258"
                     /score="31.1"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     CDS_motif       881..910
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20860_0004"
                     /evalue="1.00E-03"
                     /label="NRPS-E7"
                     /locus_tag="MOE37_RS20860"
                     /protein_end="302"
                     /protein_start="292"
                     /score="11.4"
                     /tool="antismash"
                     /translation="PEISFNYLGQ"
     aSDomain        1251..1337
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20865_NRPS-COM_Nterm.1"
                     /evalue="5.20E-16"
                     /label="MOE37_RS20865_NRPS-COM_Nterm.1"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="29"
                     /protein_start="0"
                     /score="49.6"
                     /tool="antismash"
                     /translation="MSKKSIQKVYPLTPMQEGMLYHAMLDPDS"
     gene            1251..>4343
                     /locus_tag="MOE37_RS20865"
                     /old_locus_tag="MOE37_20810"
                     /pseudo=""
     CDS             1251..>4343
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 5.2e-16.
                     Score: 49.6. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20865_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-305). E-value:
                     8.1e-114. Score: 371.7. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20865_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     6.6e-113. Score: 369.3. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20865_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1031). E-value: 2.9e-20. Score:
                     64.2. Matches aSDomain: nrpspksdomains_MOE37_RS20865_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="MOE37_RS20865"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MOE37_20810"
                     /product="amino acid adenylation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 8.6e-90, bitscore:
                     289.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-118, bitscore:
                     384.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.3e-15, bitscore:
                     44.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYPLTPMQEGMLYHAMLDPDSSSYFTQLELRIHGVFD
                     LEIFEKSVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTTVHYEDITQLDAQRQKDRL
                     HRYKQDVQQQGFNLAKDILFKVAVFRLDADHLHLVWSNHHIMMDGWSMGVLMKSLFQNY
                     EALRSGRPAVNGQGKPYSDYIKWLGKQDNEEAERYWSERLAEFEQPSVLPSRISAEKGM
                     YKSEEYSFVWDEELVSLIQQTANQHQVTGPNLFQAIWGIVLSKYNFTNDAVFGTVVSGR
                     PSDINGIETMAGLFINTIPVRVKYETESVFSDVFSAVQKHAVEAERYDYVPLYDIQKRS
                     ALDGSLLNHLVAFENYPLDKELENGSMEDRLGFSIKVDSAFEQTSFDFNLIVYPGKTWT
                     VKIKYNSAAYDTAFIKRTANHLTRMIKAAVRHPESRVLDHGLVDGAEERQMVEIFNETQ
                     TELPEEQAVHKLFEAQAERTPASLAVVYGEQELTYRQLNEAANRLARKLIEHGLQQGQT
                     AAIMNDRSAETVIGMLAVLKAGAAYVPIEPSFPEDRVRFMADDSSIRLVLTVKAYEERV
                     KWLQVPAVMMDSGSDQAVSAADLNLPARAADLAYIMYTSGSTGKPKGVMIEHRNIIRLV
                     KNAGYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKDTLLDAKGFSAFLRE
                     HRITTMWLTSPLFNQLAAKDAAMFQTLRHLIIGGDALVPHIVNQVKRVSPELSLWNGYG
                     PTENTTFSTSFLIDREYNGSIPIGKPIGNSTAYIMDEQQRLQPIGAPGELCVGGNGVAR
                     GYVNLPKLTEKQFLQDPFRPEGKIYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELG
                     EIETKISLADHVTEAAAVIRKNKADENEICVYFTADRELSAAELRKSLSQSLPEYMIPA
                     HFMQMDSLPLTANGKVDKKALPEPQSDAVQPEYAAPQTETERKLADIWEGILGVKAGVT
                     DNFFTIGGHSLKAMMMTAKIQEQFHKDVPIKVLFEKPTIQEL"
     aSDomain        1269..2165
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20865_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="8.10E-114"
                     /label="MOE37_RS20865_Condensation_DCL.1"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="305"
                     /protein_start="6"
                     /score="371.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QKVYPLTPMQEGMLYHAMLDPDSSSYFTQLELRIHGVFDLEIFEK
                     SVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTTVHYEDITQLDAQRQKDRLHRYKQD
                     VQQQGFNLAKDILFKVAVFRLDADHLHLVWSNHHIMMDGWSMGVLMKSLFQNYEALRSG
                     RPAVNGQGKPYSDYIKWLGKQDNEEAERYWSERLAEFEQPSVLPSRISAEKGMYKSEEY
                     SFVWDEELVSLIQQTANQHQVTGPNLFQAIWGIVLSKYNFTNDAVFGTVVSGRPSDING
                     IETMAGLFINTIPVRVKY"
     aSModule        1269..>4343
                     /complete
                     /domains="nrpspksdomains_MOE37_RS20865_Condensation_DCL.1"
                     /domains="nrpspksdomains_MOE37_RS20865_AMP-binding.1"
                     /domains="nrpspksdomains_MOE37_RS20865_PCP.1"
                     /locus_tags="MOE37_RS20865"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1278..1316
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0001"
                     /evalue="3.80E-06"
                     /label="C1_dual_004-017"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="22"
                     /protein_start="9"
                     /score="19.3"
                     /tool="antismash"
                     /translation="YPLTPMQEGMLYH"
     CDS_motif       1341..1457
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0002"
                     /evalue="3.30E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.1"
                     /tool="antismash"
                     /translation="SYFTQLELRIHGVFDLEIFEKSVNELIRSYDILRTVFVH"
     CDS_motif       1674..1739
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0003"
                     /evalue="1.20E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="163"
                     /protein_start="141"
                     /score="37.0"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       1785..1823
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0004"
                     /evalue="1.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="191"
                     /protein_start="178"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       2061..2156
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0005"
                     /evalue="7.00E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="302"
                     /protein_start="270"
                     /score="60.2"
                     /tool="antismash"
                     /translation="DAVFGTVVSGRPSDINGIETMAGLFINTIPVR"
     CDS_motif       2205..2321
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0006"
                     /evalue="1.20E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="357"
                     /protein_start="318"
                     /score="56.3"
                     /tool="antismash"
                     /translation="QKHAVEAERYDYVPLYDIQKRSALDGSLLNHLVAFENYP"
     aSDomain        2661..3842
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20865_AMP-binding.1"
                     /evalue="6.60E-113"
                     /label="MOE37_RS20865_AMP-binding.1"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="864"
                     /protein_start="470"
                     /score="369.3"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEAQAERTPASLAVVYGEQELTYRQLNEAANRLARKLIEHGLQQG
                     QTAAIMNDRSAETVIGMLAVLKAGAAYVPIEPSFPEDRVRFMADDSSIRLVLTVKAYEE
                     RVKWLQVPAVMMDSGSDQAVSAADLNLPARAADLAYIMYTSGSTGKPKGVMIEHRNIIR
                     LVKNAGYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKDTLLDAKGFSAFL
                     REHRITTMWLTSPLFNQLAAKDAAMFQTLRHLIIGGDALVPHIVNQVKRVSPELSLWNG
                     YGPTENTTFSTSFLIDREYNGSIPIGKPIGNSTAYIMDEQQRLQPIGAPGELCVGGNGV
                     ARGYVNLPKLTEKQFLQDPFRPEGKIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       2847..2885
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0007"
                     /evalue="9.90E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="545"
                     /protein_start="532"
                     /score="18.6"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPI"
     CDS_motif       2964..2990
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0008"
                     /evalue="3.90E+01"
                     /label="C4_DCL_171-183"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="580"
                     /protein_start="571"
                     /score="-1.1"
                     /tool="antismash"
                     /translation="YEERVKWLQ"
     CDS_motif       3075..3134
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0009"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       3636..3725
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0010"
                     /evalue="3.80E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="825"
                     /protein_start="795"
                     /score="44.8"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGNGVARGYVNLPKLTEKQF"
     CDS_motif       3807..3872
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20865_0011"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        4161..>4343
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20865_PCP.1"
                     /evalue="2.90E-20"
                     /label="MOE37_RS20865_PCP.1"
                     /locus_tag="MOE37_RS20865"
                     /protein_end="1031"
                     /protein_start="970"
                     /score="64.2"
                     /tool="antismash"
                     /translation="ERKLADIWEGILGVKAGVTDNFFTIGGHSLKAMMMTAKIQEQFHK
                     DVPIKVLFEKPTIQEL"
     gene            4384..4524
                     /gene="comS"
                     /locus_tag="MOE37_RS20870"
                     /old_locus_tag="MOE37_20815"
     CDS             4384..4524
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="MOE37_RS20870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE37_20815"
                     /product="competence protein ComS"
                     /protein_id="WP_223251813.1"
                     /transl_table=11
                     /translation="MNRYSKRLIKSIILYPRPSEGCISSISLDKQAQATTSLLYFCWKE
                     K"
     gene            <4470..>5651
                     /locus_tag="MOE37_RS20875"
                     /pseudo=""
     CDS             4470..>5651
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (1-267). E-value:
                     2e-98. Score: 321.0. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20875_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="MOE37_RS20875"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="condensation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 5.6e-86, bitscore:
                     276.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSTSYNVPAVLLLEGEVDRDRLESAIQALINRHETLRTSFDMID
                     GEVVQIIHKNVSFQLEAAKGREEDAEELIKEFIRPFELNRAPLVRSKLIQLDKERHLLL
                     IDMHHIITDGSSTGILIGDLAKLYQGADLETLKLHYKDFSVWQNDQTQKEKQKAHEEYW
                     LDTFKGNLPVLDLPTDYPRPSERSFAGERVMFGIDKQVTVQIKKLLSDTDTTMYMFLLA
                     AFHVLLAKYASQEDIVVGSPTAGRTHPDLQDVPGMFVNTVALRTSPLGEKTFKQFLDEV
                     KTISLQAFEHQSYPLEELIEQLPLTRDTSRSPLFSVLFNMQNMEIPSLRLGDLQISSYS
                     MHHHVAKFDLSLEAVERGDEIGLSFDYATALFKDETIRRWSSHFINMIKAAAEH"
     aSDomain        4473..5270
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20875_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.00E-98"
                     /label="MOE37_RS20875_Condensation_LCL.1"
                     /locus_tag="MOE37_RS20875"
                     /protein_end="267"
                     /protein_start="1"
                     /score="321.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="TSTSYNVPAVLLLEGEVDRDRLESAIQALINRHETLRTSFDMIDG
                     EVVQIIHKNVSFQLEAAKGREEDAEELIKEFIRPFELNRAPLVRSKLIQLDKERHLLLI
                     DMHHIITDGSSTGILIGDLAKLYQGADLETLKLHYKDFSVWQNDQTQKEKQKAHEEYWL
                     DTFKGNLPVLDLPTDYPRPSERSFAGERVMFGIDKQVTVQIKKLLSDTDTTMYMFLLAA
                     FHVLLAKYASQEDIVVGSPTAGRTHPDLQDVPGMFVNTVALRTS"
     aSModule        4473..7499
                     /complete
                     /domains="nrpspksdomains_MOE37_RS20875_Condensation_LCL.1"
                     /domains="nrpspksdomains_MOE37_RS20880_AMP-binding.1"
                     /domains="nrpspksdomains_MOE37_RS20880_PCP.1"
                     /locus_tags="MOE37_RS20875"
                     /locus_tags="MOE37_RS20880"
                     /monomer_pairings="Asp -> Asp"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       4479..4592
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20875_0001"
                     /evalue="3.70E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOE37_RS20875"
                     /protein_end="41"
                     /protein_start="3"
                     /score="57.9"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDRDRLESAIQALINRHETLRTSF"
     CDS_motif       4788..4853
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20875_0002"
                     /evalue="1.60E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOE37_RS20875"
                     /protein_end="128"
                     /protein_start="106"
                     /score="30.3"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKLY"
     CDS_motif       4884..4919
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20875_0003"
                     /evalue="2.80E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOE37_RS20875"
                     /protein_end="150"
                     /protein_start="138"
                     /score="13.6"
                     /tool="antismash"
                     /translation="HYKDFSVWQNDQ"
     CDS_motif       5175..5264
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20875_0004"
                     /evalue="8.30E-17"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOE37_RS20875"
                     /protein_end="265"
                     /protein_start="235"
                     /score="53.5"
                     /tool="antismash"
                     /translation="DIVVGSPTAGRTHPDLQDVPGMFVNTVALR"
     CDS_motif       5322..5432
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20875_0005"
                     /evalue="6.00E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOE37_RS20875"
                     /protein_end="321"
                     /protein_start="284"
                     /score="50.8"
                     /tool="antismash"
                     /translation="SLQAFEHQSYPLEELIEQLPLTRDTSRSPLFSVLFNM"
     gene            5658..>8996
                     /locus_tag="MOE37_RS20880"
     CDS             5658..>8996
                     /NRPS_PKS="Domain: AMP-binding (37-439). E-value: 1.8e-113.
                     Score: 371.2. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20880_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (545-614). E-value: 1e-25. Score:
                     81.7. Matches aSDomain: nrpspksdomains_MOE37_RS20880_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (629-915). E-value:
                     1.8e-105. Score: 344.2. Matches aSDomain:
                     nrpspksdomains_MOE37_RS20880_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="MOE37_RS20880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268533752.1"
                     /sec_met_domain="AMP-binding (E-value: 5.5e-122, bitscore:
                     395.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.9e-18, bitscore:
                     53.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 6.4e-95, bitscore:
                     306.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLADLELLSPAEREALLTEETHSAADVSGHTTFASLFENRAQQS
                     PERTAVFGERKLTYRELDEQANQLARHLRTHGAGSEEIVGIVMDRTVNLMVSILGVMKA
                     GAAFLPIDPDTPEERIGYSLEDSGAKIVLADERNMTAIADYQGEIVNIDTADWRRESKE
                     RLEPVAGSGNLAYVIYTSGTTGKPKGVQIEHRNLVNYVSWFSKEAGLTEGDKAVLVSSY
                     SFDLGYTSLFPVLSSGGELHIVPKETYTAPDQLGRYMKEHGITYIKLTPSLFHTIVNTS
                     RFSEGKHFDSLRLVVLGGEKIIPADVSAFHAVYADTAFINHYGPTETTIGTVAGRIDLS
                     DLAQYAERPTIGRPIANTGALVLNGALKLVPPGASGELYITGRGLARGYLRRPQLTAER
                     FIENPYSPGSLMYKTGDVVRRLSDGTIEFIGRADDQVKIRGYRIELKEIETVLLGVSGI
                     QEAVVLGVSEGGLQELCAYYKADKAYEGAEIRKQLAKKLPSHMLPAYFVQIESIPLTAN
                     GKTDRNALPKPNTAHTGHLASAAPETELEEKLCRIWQNTLGIETLGIDDNFFDLGGHSL
                     KGMMLIANIQAELEKTVPLKALFEQPTIRQLAAYLEASAQSGGYRKITPAKKREVYPVS
                     SAQKRMYVLNQLDRNGTSYNMPSVLLMEGDLNIARLQEALNQLVNRHESLRTSFAEADG
                     QPVQTIMEQAEIDLHVFEAEEAEAKQRIKAFIRPFDISSGPLIRAGLIRVGEKKHLLLL
                     DMHHIIADGVSRSIFVQELASLYKGETLPKPQLHYKDFAVWQNESAQVEQMKEHEEFWL
                     SAFAGDLPELDLPLDYPRPPVQSFKGDTIRFAAERETAKAIEELLAATGTTLHMVLHTV
                     FHVFLSKITGQKDIVIGSVTAGRTNADLQGMPGMFVNTLALRNEAGENQTFAQLLEQVK
                     HTNLEALEHQDYPFEDLIGKLELARDMSRNPLFNVMVTTEDPDKEQLQLENLKITPYQA
                     DQGTSKFDLTLGGFEDENGIGLQLEYATDLFKKETAEQWSIYLLRLLEAVAANPDQKLS
                     RLSLADEAEKQSLLDAWKGKTLSVPRDKTVHQLFEETADRFGTRQAVTYNGSSWTYSEL
                     NAKANR"
     aSDomain        5769..6974
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20880_AMP-binding.1"
                     /evalue="1.80E-113"
                     /label="MOE37_RS20880_AMP-binding.1"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="439"
                     /protein_start="37"
                     /score="371.2"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FENRAQQSPERTAVFGERKLTYRELDEQANQLARHLRTHGAGSEE
                     IVGIVMDRTVNLMVSILGVMKAGAAFLPIDPDTPEERIGYSLEDSGAKIVLADERNMTA
                     IADYQGEIVNIDTADWRRESKERLEPVAGSGNLAYVIYTSGTTGKPKGVQIEHRNLVNY
                     VSWFSKEAGLTEGDKAVLVSSYSFDLGYTSLFPVLSSGGELHIVPKETYTAPDQLGRYM
                     KEHGITYIKLTPSLFHTIVNTSRFSEGKHFDSLRLVVLGGEKIIPADVSAFHAVYADTA
                     FINHYGPTETTIGTVAGRIDLSDLAQYAERPTIGRPIANTGALVLNGALKLVPPGASGE
                     LYITGRGLARGYLRRPQLTAERFIENPYSPGSLMYKTGDVVRRLSDGTIEFIGRADDQV
                     KIR"
     CDS_motif       5952..5993
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0001"
                     /evalue="3.80E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="112"
                     /protein_start="98"
                     /score="16.9"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     CDS_motif       6180..6239
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0002"
                     /evalue="1.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="194"
                     /protein_start="174"
                     /score="33.3"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       6768..6857
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0003"
                     /evalue="1.90E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="400"
                     /protein_start="370"
                     /score="52.1"
                     /tool="antismash"
                     /translation="PPGASGELYITGRGLARGYLRRPQLTAERF"
     CDS_motif       6939..7004
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0004"
                     /evalue="4.20E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="449"
                     /protein_start="427"
                     /score="37.9"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIELKEIE"
     aSDomain        7293..7499
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20880_PCP.1"
                     /evalue="1.00E-25"
                     /label="MOE37_RS20880_PCP.1"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="614"
                     /protein_start="545"
                     /score="81.7"
                     /tool="antismash"
                     /translation="EEKLCRIWQNTLGIETLGIDDNFFDLGGHSLKGMMLIANIQAELE
                     KTVPLKALFEQPTIRQLAAYLEAS"
     aSDomain        7545..8402
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE37_RS20880_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.80E-105"
                     /label="MOE37_RS20880_Condensation_LCL.1"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="915"
                     /protein_start="629"
                     /score="344.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="EVYPVSSAQKRMYVLNQLDRNGTSYNMPSVLLMEGDLNIARLQEA
                     LNQLVNRHESLRTSFAEADGQPVQTIMEQAEIDLHVFEAEEAEAKQRIKAFIRPFDISS
                     GPLIRAGLIRVGEKKHLLLLDMHHIIADGVSRSIFVQELASLYKGETLPKPQLHYKDFA
                     VWQNESAQVEQMKEHEEFWLSAFAGDLPELDLPLDYPRPPVQSFKGDTIRFAAERETAK
                     AIEELLAATGTTLHMVLHTVFHVFLSKITGQKDIVIGSVTAGRTNADLQGMPGMFVNTL
                     ALRNE"
     CDS_motif       7611..7727
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0005"
                     /evalue="6.70E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="690"
                     /protein_start="651"
                     /score="57.1"
                     /tool="antismash"
                     /translation="TSYNMPSVLLMEGDLNIARLQEALNQLVNRHESLRTSFA"
     CDS_motif       7920..7985
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0006"
                     /evalue="9.30E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="776"
                     /protein_start="754"
                     /score="31.0"
                     /tool="antismash"
                     /translation="MHHIIADGVSRSIFVQELASLY"
     CDS_motif       8016..8048
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0007"
                     /evalue="1.50E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="797"
                     /protein_start="786"
                     /score="14.4"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       8307..8396
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0008"
                     /evalue="6.00E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="913"
                     /protein_start="883"
                     /score="47.5"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADLQGMPGMFVNTLALR"
     CDS_motif       8445..8558
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE37_RS20880_0009"
                     /evalue="4.50E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOE37_RS20880"
                     /protein_end="967"
                     /protein_start="929"
                     /score="51.2"
                     /tool="antismash"
                     /translation="KHTNLEALEHQDYPFEDLIGKLELARDMSRNPLFNVMV"
ORIGIN
        1 ctatcgcgaa gaacagggag acgtcattca atataacaga ggactcaacg aagcatctgt
       61 agatatgaag gtgttcaaac tcaacgggcc ggcgattgac aatgaggaaa aaatcgagcg
      121 tgaagcagac catctccaaa gcagcattgt attggagact ggcaatctgt taaaagcggg
      181 gcttttccaa gcggaggacg gcgaccattt actgcttgca gttcatcact tagcggtaga
      241 cggtgtttct tggcggattt tactggagga tttcgctgct gtctataccc agttaaagca
      301 aggaaataag ccggtccttc cgcagaagac ccattcattt gccgagtatg ccgagaggtt
      361 aaaagaattc gccaacacga aggccttctt aaaggaaacg gattattgga gacaactaga
      421 ggaaaacaca attcagacgc cgcttccgaa agaccgtcaa tccagcgatc agcgaatgaa
      481 gcatacaaga acggttcaat tctcgctgac tgccgaagaa acagaaaagc tgacaacaaa
      541 ggttcacgag gcttatcaca cggaaatgaa tgatatttta ctgacagcgc tcggtttggc
      601 aatgaagaac tggacaggcc aagaccaagt aagtgttcac ttagagggac atggccgtga
      661 agagatactc gaagatatat ccatttcgcg gactgtcggc tggtttacaa gcatgtaccc
      721 gatggtgctc aacatgaagc atgccgacga tttggcatat cagctaaagc aaatgaaaga
      781 agatatcaga cacgttccga acaaaggcgt cggctacggc attttacgtt atctgacagc
      841 gccggaacat aaaacagaac tcgaattctc tattcagccg gagatcagct tcaactactt
      901 agggcaattt aacgaaatgg cggattccgg attgtttaca agatcctcaa tgccatctgg
      961 acaatcatta agcccggaga cagaaaaacc gaatgcgctg gatatcgtcg gttatatcga
     1021 gaatggaaca ctgacgatga cactggccta tcattctctg gaatttcatg aaagaaccat
     1081 tcaggctttc agcgacagct ttaaaaccca tttactcaaa gtaatggagc attgcttagc
     1141 ccaagatggc acagtgctta cgcctagtga tcttggtgat gatgatctga cacttgatga
     1201 gcttgataaa ttaatggaaa ttttttaata gaaaaagaca gaggtggcat atgagcaaaa
     1261 aatcgattca gaaggtgtac ccgttgacac cgatgcagga agggatgctg tatcatgcga
     1321 tgcttgatcc ggattcttcc tcttatttca cacaattaga gctgcgcatt cacggtgtct
     1381 ttgaccttga aatctttgaa aaaagcgtca atgagctgat acggtcatac gatattcttc
     1441 gcactgtgtt tgtgcatcag cagcttcaaa agccccgcca agtcgtattg gcggagcgta
     1501 agaccacagt gcattatgaa gatataaccc aattagacgc ccagcgtcaa aaagatcgtc
     1561 ttcaccgtta taaacaggac gtgcagcagc agggctttaa tctggcgaag gatatcctgt
     1621 ttaaagtggc ggttttccgg ctggatgcgg atcatctgca tcttgtctgg agcaatcatc
     1681 atattatgat ggacggctgg agtatgggag tgctcatgaa aagcttgttc caaaactatg
     1741 aagcgctccg ctcgggccgc ccggctgtca atggacaagg caagccttac tccgactata
     1801 ttaaatggct aggaaagcaa gacaatgaag aagcggaacg ctattggagc gagcgcttgg
     1861 ctgagtttga acagccaagc gtgctcccga gccgcatttc tgccgaaaag ggcatgtaca
     1921 aaagcgaaga gtacagcttt gtatgggatg aggagctggt atcgcttatt cagcaaacgg
     1981 cgaatcaaca tcaggttaca ggacccaacc tttttcaagc tatttgggga attgttctca
     2041 gcaaatacaa ctttacgaac gatgccgtct tcggcacagt ggtttccgga cgtccatctg
     2101 acattaacgg gattgagaca atggcggggc tgttcattaa caccataccg gtccgcgtaa
     2161 agtatgaaac tgaatctgtc ttctccgatg tattttcagc cgtacaaaag catgccgtcg
     2221 aagcagagcg ttatgattac gtgccgctct acgacattca aaagcgttca gcgctggacg
     2281 gaagcctgct taaccatctt gtcgcttttg aaaactatcc tcttgataaa gagctggaaa
     2341 acggaagtat ggaagaccgt ctcggctttt ccattaaggt agacagcgct tttgaacaga
     2401 caagttttga ttttaacctg atcgtgtatc cggggaaaac atggacggtc aaaatcaaat
     2461 ataacagcgc cgcgtacgat acggcattta taaaaagaac agcgaaccat ctgacacgca
     2521 tgatcaaagc ggctgtccgg catccggaat cccgcgtgct ggatcacggg cttgtggatg
     2581 gagcggaaga acggcagatg gtggaaattt ttaacgaaac gcagacagag cttccggaag
     2641 aacaagccgt tcacaaattg tttgaagcgc aagcggaacg gacgccggca agccttgctg
     2701 tcgtatacgg ggagcaagag ctgacatacc ggcagctgaa tgaagcggcg aatcgtctgg
     2761 caagaaaact tatcgaacat ggcttgcagc aaggacagac agctgcaatt atgaatgacc
     2821 gttcggccga aaccgtgatc ggcatgcttg ccgtattgaa ggcaggcgcc gcatatgtgc
     2881 cgattgagcc atccttccct gaggaccggg ttcgcttcat ggcggacgac agttccatcc
     2941 ggcttgtgct gactgtaaag gcatatgaag agcgggtgaa atggttacaa gtccctgccg
     3001 ttatgatgga cagcggctca gatcaggctg tgagcgccgc tgatctgaac ttgcctgccc
     3061 gcgctgcgga tttggcatat attatgtaca cttccggttc taccgggaag ccaaaaggcg
     3121 tcatgattga acacagaaat atcatacgac tggtgaaaaa cgctgggtat gttcccgttc
     3181 atgaagagga tcggatggct cagacggggg ctgtcagttt tgatgccgga acgtttgagg
     3241 tgtttggcgc attgttaaac ggcgcggcgc tttatcctgt caaaaaggat acgctgctgg
     3301 acgcgaaggg cttttcggca ttcctgcgtg agcatcgcat tacgacgatg tggctgacgt
     3361 ctcctttatt caaccagctg gccgcaaagg atgccgccat gttccaaacg cttcgccatt
     3421 taatcatagg cggcgatgct ttagttccgc atatcgtgaa ccaagtcaaa cgggtttctc
     3481 cggagctttc tctttggaac ggatacggac cgacagagaa tacgactttt tcaacgagct
     3541 ttcttattga tcgggaatac aatggctcga taccgatcgg gaaaccaatc ggaaactcca
     3601 ccgcttacat tatggatgaa cagcagcgcc ttcagccgat cggggcacca ggcgagctgt
     3661 gtgtcggcgg aaacggagtg gcgcggggct atgtaaactt acctaaactg acagaaaagc
     3721 aatttctcca agatcctttc agaccagagg gaaaaatata ccggacaggt gatttggcga
     3781 gatggcttcc ggacggaaat atcgagttct taggaagaat tgataatcag gtaaaagtcc
     3841 gcggcttcag aatcgagctg ggtgagattg aaacaaaaat cagtttggca gaccacgtga
     3901 cagaggctgc cgctgtcatt agaaaaaata aagcggatga aaacgaaatt tgtgtgtatt
     3961 tcaccgctga tcgtgagctg tctgcggctg agctccgtaa gtcgctgtcg caatctttgc
     4021 cggaatatat gattccagct cattttatgc aaatggacag cctgccgctc acggcgaacg
     4081 gaaaggtcga taaaaaagcg ctgccggagc ctcagtcaga tgccgtgcag ccggaatatg
     4141 ccgcgccgca gacggagacg gagagaaaat tagcagacat ttgggaaggg attctcggcg
     4201 taaaagccgg agttaccgac aatttcttta cgatcggcgg ccattcgctt aaagcgatga
     4261 tgatgacggc taaaatccaa gagcagtttc ataaggacgt tccgattaaa gtgctgtttg
     4321 aaaagccgac gatccaggaa ttggcacaat ttttggaaga aaacaacaag gaggaacagc
     4381 gttttgaacc gatacagcaa gcgccttatc aagagcatta tcctgtatcc tcggcccagc
     4441 gaaggatgta tatcctcaat cagcttggac aaacaagcac aagctacaac gtccctgctg
     4501 tacttctgct ggaaggagaa gtagacagag atcgtcttga atccgcgatt caggcgttaa
     4561 tcaaccgcca tgaaacattg cgcacttctt ttgacatgat agacggagaa gtcgtgcaga
     4621 tcattcataa aaatgtttct tttcagctgg aggctgccaa gggccgggaa gaagacgcgg
     4681 aagaactgat caaagaattt atccggccgt ttgagttaaa ccgcgcgccg ctcgtccgtt
     4741 cgaagctgat ccagcttgat aaagaacgcc atctgctgct gattgatatg caccacatta
     4801 ttacagacgg cagctcgacg ggcattttaa tcggggatct ggctaaattg tatcaaggcg
     4861 cggacttaga aacgcttaag cttcactata aagatttttc tgtctggcaa aacgatcaaa
     4921 cacaaaaaga aaaacaaaaa gcgcacgaag aatattggct ggatacattt aaagggaatt
     4981 tgccggttct cgatctgccg acggattatc cgcgtccgtc cgaacgcagc tttgccggag
     5041 aacgggtcat gttcggcatc gacaaacaag tcaccgtaca gattaagaag ctgctgtctg
     5101 acacggacac aacgatgtat atgttcttat tggccgcttt ccatgtattg ctggctaaat
     5161 atgcatcaca ggaagatatt gttgtcggct ctccgaccgc gggcagaacg catcctgatc
     5221 ttcaggacgt acctggaatg tttgtcaaca cggtggcttt acggacatcg cctttaggtg
     5281 agaaaacctt taaacagttc ttagatgaag tgaaaacaat cagccttcag gcatttgagc
     5341 accagagcta tccgctcgag gaattaatcg aacaactgcc gctgacaaga gacacaagca
     5401 ggagtccgct gttcagcgtg ttatttaaca tgcaaaacat ggagattccg tccttaagat
     5461 taggagattt gcagatttcc tcttattcca tgcatcatca tgtggctaaa ttcgatttat
     5521 ctttagaagc ggtggagcgc ggggacgaga ttggtttaag cttcgattac gcgacagctt
     5581 tgtttaaaga cgagaccatc cggcgttgga gcagccattt cataaacatg ataaaagctg
     5641 ctgccgaaca tccgaatatg aggctcgctg atttggaact gctctcccca gcggagcggg
     5701 aagctcttct gactgaagaa acacattcag cagcggacgt gtcggggcat acgacatttg
     5761 catctttatt tgaaaataga gcgcagcagt cacctgaacg gacagcggtt ttcggtgaaa
     5821 ggaaattgac ctatcgcgag ctggacgagc aggcaaacca gctggcgcgt catctccgca
     5881 cacatggagc cggcagtgaa gagatcgtcg gcattgtcat ggaccggact gtcaatttga
     5941 tggtgtctat tctcggcgtc atgaaggccg gtgccgcttt ccttccgatt gatccggata
     6001 cacctgaaga aagaatcggt tattcattgg aagacagcgg agcgaagatt gttctcgctg
     6061 acgagagaaa tatgacggcc attgccgatt atcaaggtga aatagtcaat attgatacgg
     6121 ctgattggcg ccgggaaagc aaggagcggc tagaacctgt tgcaggttct ggcaatcttg
     6181 cttatgtcat ttacacatcc ggtacgaccg gaaaacctaa aggcgtgcag attgaacatc
     6241 gcaatctcgt gaactatgtg tcttggtttt caaaggaggc cgggctgaca gaaggagata
     6301 aagcggtact ggtgtcgtcc tactcatttg atttgggcta cacaagtctg tttcctgttc
     6361 tctcctcagg aggcgagctt catatcgtgc caaaagagac atacaccgcg ccggatcagc
     6421 ttgggcgtta tatgaaggaa cacggcatta cctatatcaa actgacaccg tcactgtttc
     6481 acacgattgt caacacctcc cgtttttcag aagggaagca ttttgactcg ctccgtttgg
     6541 ttgtgctcgg cggagagaaa attattccgg ctgatgtcag cgccttccat gcggtctatg
     6601 ccgatacagc ttttatcaat cattacggtc cgacagaaac aacgatcggc acagtcgccg
     6661 gacgcattga tctgtcagac ctggcgcaat acgcagaacg tccgacaatc gggcggccga
     6721 ttgccaatac aggcgctctt gtgctaaacg gggcattaaa gcttgttcct ccgggagcga
     6781 gcggcgagct ctatattacg ggccgggggc ttgccagagg gtacctccgc cgtccgcagc
     6841 tcacagccga gagattcatt gaaaatccat attcaccggg aagcttgatg tacaagaccg
     6901 gagacgtggt gcgaagactt tctgacggaa cgattgaatt tatcggacgt gccgatgatc
     6961 aagtgaaaat ccgcggatac cgtatcgaac tgaaagaaat tgaaaccgtt ctgctcggag
     7021 taagcggcat tcaagaagcg gtggttctgg gggtttcaga aggcggtctt caggaacttt
     7081 gcgcttatta taaagcggac aaagcgtatg aaggggctga aattcgcaag cagcttgcga
     7141 agaagcttcc gtctcacatg ctgccggcgt actttgtgca gattgagtca atcccgctca
     7201 ccgcaaatgg gaaaaccgac agaaatgctt tgccgaaacc aaacacggcc cataccgggc
     7261 atctcgcatc ggctgctccg gaaactgagc tggaagagaa gctttgccgt atttggcaga
     7321 ataccctcgg cattgaaacg cttggcatag atgataactt ctttgatctc ggcggccatt
     7381 ccttaaaagg aatgatgctg atcgccaata ttcaggctga gctcgaaaaa acagtaccgt
     7441 taaaagcact ttttgagcaa ccgacgattc gtcagctggc tgcctatttg gaagcctcgg
     7501 ctcaatcggg cggttatcgg aagattaccc ccgccaaaaa gcgtgaggtc tatccggtgt
     7561 cttcggctca aaaacggatg tatgtgttga accagcttga ccgaaacggc acgagctata
     7621 atatgccgtc cgttcttctg atggaaggcg atctcaacat cgcgcgtttg caggaggcgc
     7681 tcaatcagct tgtgaaccgc catgaatcgc tgcgaacctc atttgctgag gctgacggcc
     7741 agcccgtcca aacgatcatg gagcaagctg aaatagacct gcatgtgttt gaagctgaag
     7801 aagcggaagc aaaacagcgg atcaaagcat ttatccgtcc gtttgatata agctcaggac
     7861 cgctcatccg cgctggattg atccgcgtgg gagagaagaa acaccttctg ctattggata
     7921 tgcatcatat tatcgctgac ggagtttcca gaagtatttt tgtacaagaa ctggcatcgc
     7981 tgtataaggg agaaaccctc cctaaaccgc agcttcatta taaagatttt gcagtttggc
     8041 agaatgaatc agcgcaagtt gaacagatga aggaacacga ggagttctgg ctctcggctt
     8101 ttgcgggcga tcttccggag cttgaccttc cgcttgatta tccccgtcct cccgtacaga
     8161 gctttaaagg ggacacgatc cgcttcgcag cagaaagaga gacggcgaag gcgattgaag
     8221 aattgctggc cgctaccgga acaaccttgc acatggtgct gcataccgta ttccatgtct
     8281 ttttaagcaa aatcaccggc caaaaggata ttgtcatcgg ctcagttaca gcaggcagaa
     8341 caaatgctga tttgcaggga atgccaggta tgttcgtcaa tacgcttgcg ctgcgcaacg
     8401 aagcaggtga aaaccaaacc tttgctcagc ttcttgagca agtgaaacat acgaatcttg
     8461 aggcattgga gcatcaggac tatccattcg aagacttgat cggaaagctt gaactggcaa
     8521 gggatatgag ccgaaaccca ttgttcaatg tcatggtgac aacggaagac cctgataaag
     8581 aacagcttca gctcgaaaat ctgaaaatca ccccgtatca agcagatcaa ggcacgtcta
     8641 aatttgatct gactctcggc gggtttgagg atgaaaacgg tattggcctt cagctggaat
     8701 atgcgaccga tttatttaaa aaagaaacgg ccgaacaatg gagcatatat cttttgcggc
     8761 tgttagaagc ggttgccgcc aaccctgacc aaaaactctc acgcctgtca ctggctgacg
     8821 aagcagaaaa acaaagcctg cttgatgcat ggaaaggcaa aacgctttct gtgccgcggg
     8881 acaaaacagt tcatcagcta tttgaagaaa ccgctgatcg cttcggcact cgccaagccg
     8941 tcacgtacaa cggaagctca tggacataca gcgagctgaa cgccaaagcg aaccgt
//
