LOCUS       NZ_JALAUD010000003     11949 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus atrophaeus strain CK5F-3 Contig_0000003, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAUD010000003
VERSION     NZ_JALAUD010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1227820)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1227820)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 424.2897441031268x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:37:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,211
            CDSs (total)                      :: 4,134
            Genes (coding)                    :: 3,990
            CDSs (with protein)               :: 3,990
            Genes (RNA)                       :: 77
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 85 of 144
            Pseudo Genes (incomplete)         :: 104 of 144
            Pseudo Genes (internal stop)      :: 21 of 144
            Pseudo Genes (multiple problems)  :: 58 of 144
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:44:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1215871
            Orig. end                         :: 1227820
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAUD010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11949
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="4"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..11949
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11949
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:11890](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11890
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(439..2028)
                     /locus_tag="MOE28_RS13280"
                     /old_locus_tag="MOE28_13255"
     CDS             complement(439..2028)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019714632.1"
                     /locus_tag="MOE28_RS13280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13255"
                     /product="solute:sodium symporter family transporter"
                     /protein_id="WP_268486907.1"
                     /transl_table=11
                     /translation="MIWIIVSFLLINIGIFLFSWLRTKDVNLHTSDGYFLGGRSLTALY
                     IGSSLLLTNLSTEQMVGLNGQSYAGSMTAMSWEASAPIALIFFALLFLPRYLKTGITTI
                     PDFLEKRFDQKTRQIVSLLLIIGYAVSFLPTVLYSGALVLDQVFGFTDNFGISTFTAVL
                     IISFIIGITSCCYIFFGGLKAAASADAIYGIGLICGGLLIVVLGILAIGEGNFGGGVAK
                     LTAVHTEKLNSIDISNSAEVPWPTILTGLLVNNLFYWAVNQAIVQRALGAKNLAEGQKG
                     ALLAGFFKLVGVFYLVLPGIIAFHLYGGGINNADSVYPRLIVDLLPTALTGIFAAILFG
                     AILSSFNGSLNSTITLFTLDIYKPIFKPNANDADLVKTGRIFVAILGVVSIIIAPFILY
                     APSGLYNYLQEMFGFFNVPILAAVVVGFFSKRVPALAVKIAIPAHIVLYGLSKIYLGHI
                     HFLYILAVLFPFSVAVMLFIGRLRPREKEYRLNHDPQVDLKPWKHVKLASCIILVMVSV
                     IYIVFSPWGIAG"
     gene            complement(2208..3113)
                     /locus_tag="MOE28_RS13285"
                     /old_locus_tag="MOE28_13260"
     CDS             complement(2208..3113)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389802.1"
                     /locus_tag="MOE28_RS13285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13260"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_268480943.1"
                     /transl_table=11
                     /translation="MKKTIMSFVAVAAISSTAFGAHASAKEITVQKGDTLWGISQKNGV
                     NLKDLKEWNQLSTDIIIAGEKLTISSEEETTAGQYTVKEGDSLWKIAQKYGISINSLKS
                     WNNLDSDMIYAGTTLAVKGQVSASNTAQESQPQAEPKQEAVQTEQPKQETVQQQPKQET
                     VQQQPKQETVQQQPKQESKAAAETSEKTEEQPVQSNTSNQNAKKELTVTATAYSANDGG
                     ISGVTATGVDLNKNPNAKVIAVDPKVIPLGSKVYVEGYGEATAADTGGAIKGNKIDVFV
                     PKTSDAHNWGVRTVKVKVLN"
     gene            complement(3361..5139)
                     /gene="recQ"
                     /locus_tag="MOE28_RS13290"
                     /old_locus_tag="MOE28_13265"
     CDS             complement(3361..5139)
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003325807.1"
                     /locus_tag="MOE28_RS13290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13265"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_268371143.1"
                     /transl_table=11
                     /translation="MLEKARSLLAHYFGYEKLRPGQEQAIRSVTEAKKNTACIMPTGGG
                     KSICYQIPALLFEGTTIVISPLISLMKDQVDALEEAGIAAAYINSTQSNQDIQSRLTAL
                     KNGSYHLFYITPERLTSPEFIRILQSITVPLVAIDEAHCISQWGHDFRPSYRNIEILFR
                     ELKEKPVIMALTATATPEVHEDICKQLHIEKENTVYTGFSRDNLTFKVAKGENKDRFIE
                     EYLNRNKLEAGIIYTATRKEADRICEKLKRQHINAGRYHGGLPDEVRKEQQEQFLNDQI
                     QVMVATSAFGMGIDKSNIRFVLHYQIPKDMESYYQEAGRAGRDGLDSECILLFSPQDIM
                     VQRFLIEQSASDEGKQKQDLKKLRQMVDYCHTEDCLQRFILKYFGEVDPAACGQCGNCT
                     DTRQAHDVTKEAQMVLSCMIRMNERFGKTMIAQVLAGSKNKKVLDNGFSRLSTYGILKH
                     QSVSEISDFIEFLISDDFIQMSDGTFPTLFVTNKGRNVLLGKEDVVRKEALKAAAITEN
                     DALFERLRMLRKQIAAEQGVPPFVVFSDQTLKEMSGKQPANEEDLLSIKGVGEQKRVKY
                     GKLFLEEIQAYVSETV"
     ncRNA           complement(5272..5458)
                     /db_xref="RFAM:RF00013"
                     /gene="ssrS"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /locus_tag="MOE28_RS13295"
                     /ncRNA_class="other"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="MOE28_13270"
                     /product="6S RNA"
     gene            complement(5272..5458)
                     /gene="ssrS"
                     /locus_tag="MOE28_RS13295"
                     /old_locus_tag="MOE28_13270"
     misc_feature    complement(5493..5509)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.04"
                     /tool="antismash"
     gene            complement(5663..6289)
                     /locus_tag="MOE28_RS13300"
                     /old_locus_tag="MOE28_13275"
     CDS             complement(5663..6289)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389804.1"
                     /locus_tag="MOE28_RS13300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13275"
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="WP_268391813.1"
                     /transl_table=11
                     /translation="MSQVLFVKASDRTAEEGVSVKLYEAFLAAYKENNPNDEVIELDLH
                     KENLPYLGRDMINGTFKAAKGIEMSEEEKKQASTADVYLNQFVKADKVIFAFPLWNFTV
                     PAVLHTYVDYLSRAGVTFTYTPEGPKGLMGDKKVALLNARGGVYSEGPMAALEMSLNFM
                     KTVLGFWGVQDLHTVVIEGHNAAPDNAQTIVEKGLQEAKDLAAKF"
     gene            complement(6444..6935)
                     /locus_tag="MOE28_RS13305"
                     /old_locus_tag="MOE28_13280"
     CDS             complement(6444..6935)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389805.2"
                     /locus_tag="MOE28_RS13305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13280"
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="WP_010788918.1"
                     /transl_table=11
                     /translation="MALTKEQTQHLYHKLLDLQKELSGPKKATESMTEEAGELSNGVDN
                     HMADHGSLYTDRMTDQTVKEVDRELLDEVNNALQKIKDGTYGICEATGEEIPYERLDAV
                     PYARMTVEAQTDREEALEPDAPAVEKEFHAQVKDLSNKETIDQKSSATYEVLDREQDSK
                     "
     gene            complement(7078..7422)
                     /locus_tag="MOE28_RS13310"
                     /old_locus_tag="MOE28_13285"
     CDS             complement(7078..7422)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389811.1"
                     /locus_tag="MOE28_RS13310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13285"
                     /product="PH domain-containing protein"
                     /protein_id="WP_003325803.1"
                     /transl_table=11
                     /translation="MGIFSVSRPTDAKKLEALLVEGERIESIYKLRVDQICFTNKRIIF
                     FDNKMFSKKKVRVFLPYKTIESFAIQEAGMFDPDTGLLLMTRSKTFDLEFAKDTDLSEV
                     QAILTKYLCS"
     misc_feature    complement(7655..7668)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     misc_feature    7655..7669
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.7"
                     /tool="antismash"
     misc_feature    7674..7688
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.9"
                     /tool="antismash"
     gene            complement(7756..7899)
                     /locus_tag="MOE28_RS13315"
                     /old_locus_tag="MOE28_13290"
     CDS             complement(7756..7899)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003325802.1"
                     /locus_tag="MOE28_RS13315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13290"
                     /product="DUF4025 domain-containing protein"
                     /protein_id="WP_010788920.1"
                     /transl_table=11
                     /translation="MKRQVNNGKEEAENFPEQKHPTEGEDVTREQISDTYYEGTIEDTD
                     RD"
     gene            complement(7969..8172)
                     /locus_tag="MOE28_RS13320"
                     /old_locus_tag="MOE28_13295"
     CDS             complement(7969..8172)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389812.1"
                     /locus_tag="MOE28_RS13320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13295"
                     /product="DUF6501 family protein"
                     /protein_id="WP_003325801.1"
                     /transl_table=11
                     /translation="MIHKTWLKKETVRKVKCVQTNAKKYIVNRVLTPGKEYEVKNETEE
                     FLFVVDNSNKVGGFYKEYFEEV"
     gene            8404..9891
                     /locus_tag="MOE28_RS13325"
                     /old_locus_tag="MOE28_13300"
     CDS             8404..9891
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389813.1"
                     /locus_tag="MOE28_RS13325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13300"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_268521541.1"
                     /sec_met_domain="Aldedh (E-value: 1.3e-192, bitscore:
                     636.6, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSLTMQISKRLETFLQGTKKLYIDGKFVPSASGATFVTPNPATG
                     ETLMTLFEAQAEDVDKAVKAARKAFDQGEWRTMPAASRSRLMYKLADLMEEHQTELAQL
                     ETLDNGKPISETTNGDIPLAIEHMRYYAGWSTKITGQTIPVAGSYFNYTKHDPVGVVGQ
                     IIPWNFPLLMAMWKMGAALATGCTIILKPAEQTPLTALYLAELIDEAGFPDGVINIIPG
                     FGETAGEALSNHEEVDKIAFTGSTEIGKKIMETAAKSIKRVTLELGGKSPNIILPDANL
                     KKAIPGALNGVMFNQGQVCCAGSRVFIQKDRYDEVVSEMVTYAESLRQGAGLHKDTQIG
                     PLVSKEQHERVLSYIQKGKDEGAKAVTGGSCPYEEGYFVSPTVFTNVEDGMTIAKEEIF
                     GPVLTAIPYDTVDEVIERANNTEYGLAAGLWTENLKHAHYIADRLQAGTVWVNCYNVFD
                     AASPFGGYKQSGLGREMGSYALNNYTEVKSVWINLED"
     gene            10001..11890
                     /locus_tag="MOE28_RS13330"
                     /old_locus_tag="MOE28_13305"
     CDS             10001..11890
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015388120.1"
                     /locus_tag="MOE28_RS13330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE28_13305"
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="WP_268521542.1"
                     /sec_met_domain="T2TS (E-value: 2.8e-116, bitscore: 385.3,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTLQEQVARCQKKTITQLKNMQNADGSWSFCFEGPIMTNSFFIL
                     LLTSFEDKGNEELIVKLAKGIREKQRPDGTFTNYPDEEKGNVTATVQGYIGMLASGCYL
                     RSDPHMIQAERFIISQGGLKDVHFMTKWMLAANGLYPWPALYLPLCLLAIPPTFPLHFY
                     QFSTYARIHFVPMAVTLNQRFFLKNPNIPSLKYLDSHMSKNPFTWLRTDAYDQRDLSSI
                     LAYWKQLLNVPFSLHQLGFRTAKTYMLDRIEKDGTLYSYASATIFMIYSLLSLGMSRHS
                     SIIQQALNGVKTLVTECDGIPYLENSTSAVWDTALISYALQQSGVPEKDLMITAAADFL
                     LERQHKKKADWSVKNPHAAPGGWGFSKINTINPDCDDTAAALQAIPRALSPMSWDRGLN
                     WLLSMQNKDGGFSAFERNVNHPLIRLLPLESAEDAAVDPSTPDLTGRVLRFLGEKAGMT
                     QDNRQIKKAVNWLIDNQEDNGSWYGRWGVCYIYGTWAAITGMRACGTEAFHPAVKKAVH
                     WLKTIQHDDGGWGESCRSAEVKTYVPLRYGTVTQTAWALDALLQYEEPDHPSITKGLEF
                     LINSENHNKHSFDYPTGIGLPKQFYIRYHSYPFAFSLLAYSSYMKSIEKREMK"
ORIGIN
        1 actatatttc tatgatactc gataagcttg aagtgaaaaa ccggattgaa gccattaccc
       61 ggtcaaagga aaaaggctgg tttaaataaa aaaagagcat cttggtcatg tccgagatac
      121 tcttttttat tgaatcagcc ttttcttttt atttaggccc ctgcttcaag tgtcacctgc
      181 acatcaaaat aagggctgta ttctgattca ataaggtaag tctggcagaa gttgtccgca
      241 agtaaacata tatcacaggc gcatgctccg atcaaatgac tgcttcccct gctctcttcg
      301 caacaaaacg gcttttaatc tctgtttttc aacctcataa agatataagc aaaagcattc
      361 cgtcttaggg ggttaacccc ttttctattt acttcgcggt tcagctccat ctcccccctt
      421 tctatttaga attttgtttt aaccggcaat cccccacgga gaaaagacaa tataaatgac
      481 agacaccatc acgagaataa tacatgacgc aagttttaca tgcttccaag gtttcaagtc
      541 cacttgagga tcatggttaa gacgatactc tttttctctg ggccgaagcc ttccgataaa
      601 cagcatcacc gccaccgaga acggaaagag gacagctagg atatacagaa aatgaatgtg
      661 ccccaaatag attttagata agccatacag gacaatatga gccggtatcg caattttaac
      721 ggctaaggcg ggtactctct tagaaaagaa ccccaccaca actgcagcta agatcggcac
      781 attaaaaaag ccaaacattt cttgaagata attatacaag cctgatggcg catacaaaat
      841 aaatggcgca atgataatgg agacaacgcc aagaattgct acaaatattc tgcctgtctt
      901 tacaagatca gcatcatttg cattcggttt aaaaatcggc ttataaatat caagtgtaaa
      961 taatgtaatc gtactattca atgaaccgtt aaacgagctt aaaatagcgc cgaataagat
     1021 agctgcgaaa ataccggtaa gcgctgtcgg cagtaaatct acgatcaggc gcggatagac
     1081 actgtcggcg ttattgattc cgcctccata aagatgaaaa gcaataatac ccggcaggac
     1141 taaataaaaa accccgacta atttaaaaaa tcctgctaag agcgcccctt tttgcccctc
     1201 agccaaattt ttagcgccta atgcgcgctg aacgatcgcc tggttgacag cccagtaaaa
     1261 caaattattt actaagaggc ccgttaaaat ggttggccat ggcacttctg cggagttgct
     1321 gatatcgatt gaattaagct tctctgtatg tacggccgtt aatttggcaa caccgcctcc
     1381 aaagtttcct tcaccgatag ctaatatacc gagcacaaca atcagcaagc cgccgcagat
     1441 caaaccgatt ccatagatgg cgtctgctga agccgcagct ttcaaaccgc cgaagaagat
     1501 ataacagcag ctcgttatgc caatgataaa tgaaatgatc aatacggctg taaaagtgct
     1561 gattccaaaa ttatccgtaa aaccgaaaac ctgatctaat acaagtgccc cggagtataa
     1621 aacagtcgga aggaatgaaa ccgcatagcc gatgatcagc aacaaagaga caatttgtct
     1681 tgttttttga tcaaagcgct tttcaagaaa atcgggtatt gtcgttatgc ctgtttttaa
     1741 atatcgcgga agaaaaagta acgcgaaaaa tatcaaagca atcggcgcac tcgcctccca
     1801 agacattgca gtcatcgaac cggcgtagct ttgaccgttt aatccaacca tttgttctgt
     1861 cgatagattg gtaagcaata aggaactccc tatatacaac gcagtaaggc ttctccctcc
     1921 gagaaaatag ccgtcagacg tatgtaagtt cacatccttc gttcttagcc acgagaacaa
     1981 aaatatgccg atattaatga gtaaaaaaga tacgataatc catatcaagt ttctgcatcc
     2041 cctttttttc aaaacataag acccttattc cctgtttacg aacagaaaaa acttgagaag
     2101 cacattcgaa cttttcagaa tataatggtt aggaaagaga aaaaaaggga agaaaaaaag
     2161 gacacgagct tacgctcagt gtccctgatt taaaaaccga tatatgacta gttaagaact
     2221 tttaccttta cagttctgac gccccagtta tgtgcgtcac ttgttttagg tacaaataca
     2281 tctattttat ttccttttat tgcgccgcca gtatctgctg cagttgcttc tccgtaacct
     2341 tcaacgtaga cttttgaacc aagcggaatc actttaggat ccaccgcaat tacttttgcg
     2401 ttagggtttt tattcaagtc tacacctgtg gccgtaacgc cggaaatgcc gccgtcgtta
     2461 gctgaataag cagttgcagt aactgttagt tcttttttag cattttgatt gcttgtattt
     2521 gattgaacag gctgttcctc tgttttctca gaagtttctg ctgcagcctt cgattcttgt
     2581 ttaggttgtt gttggacagt ttcttgttta ggttgttgtt ggacagtttc ttgtttaggt
     2641 tgttgttgaa cagtttcttg ttttggctgc tctgtttgca ccgcttcttg ttttggctca
     2701 gcttgaggct ggctctcttg agctgtgttt gaagctgata cctgaccttt aaccgcaagt
     2761 gttgttcctg catagatcat gtcagagtct agattgttcc agcttttaag gctgttgatt
     2821 gaaatgccgt atttttgagc aatcttccag aggctgtccc cctctttaac tgtgtattgc
     2881 ccagcggttg tttcttcttc tgaagaaatg gtcagtttct ctcctgcaat gattatatca
     2941 gtagatagct gattccattc ttttaagtcc tttaggttca ctccgttttt ctgagagatt
     3001 ccccagagcg tgtcaccctt ttgcaccgtg atttctttcg cagaagcgtg agctccgaat
     3061 gcagttgatg agattgctgc aactgcgaca aaggacataa tcgtcttctt cataagtaaa
     3121 tcctcccttg ttagcttttt attggcggct aacaggggat atcgtaacat atgtaaatgt
     3181 catttcaata acaaaacgat ttgaattaga ttacagttcc tttacatgaa agatttctct
     3241 tggttggaaa gacttgctga acccttggta taaagcggtt ttacaagtct tatttgcatg
     3301 gctttttccg ctccggacaa aatgtgtttt ctccaaaaaa aacttatttt ttttgctttt
     3361 ttatactgtt tcacttacgt aagcttgaat ttcttctaaa aaaagcttgc cgtatttcac
     3421 ccttttttgt tctcccactc ctttaattga aagaagatcc tcttcattcg caggctgctt
     3481 gcctgacatt tcttttagcg tttgatcaga aaaaacgaca aatggcggga cgccttgttc
     3541 tgcggcaatt tgctttcgaa gcatgcgaag ccgctcaaac aaggcgtcat tttcagtaat
     3601 cgcagccgct ttgagcgctt cttttctgac aacgtcctct tttccaagca gcacgtttct
     3661 tcctttattg gttacaaaaa gagtcggaaa cgtaccgtct gacatttgaa taaagtcatc
     3721 agaaatcaga aactcgataa aatcgctgat ttcagataca gattgatgct ttaaaatccc
     3781 gtacgtcgat agccggctga acccgttatc cagcactttt ttgttttttg agcccgccag
     3841 cacctgagcg atcatggttt tgccgaatcg ttcattcata cgtatcatac acgataatac
     3901 catttgcgct tctttggtga cgtcatgcgc ttgccttgta tctgtacagt tgccgcattg
     3961 tccgcaggct gccggatcaa cctcaccgaa atatttaaga ataaatcgct gcaagcaatc
     4021 ttctgtgtgg caataatcaa ccatttgcct cagtttcttc agatcctgct tttgctttcc
     4081 ttcatctgat gccgattgtt cgattaaaaa gcgctgcacc ataatatcct gcggtgaaaa
     4141 cagaaggata cattcactgt caagcccgtc acggcctgcc cttcccgcct cttgatagta
     4201 gctttccata tcttttggaa tttgataatg caaaacaaat ctgatattgg atttgtcgat
     4261 gcccatacca aaagcagacg ttgcgaccat cacttggatt tggtcattaa gaaactgctc
     4321 ctgctgctct ttcctgactt catccggcaa tccgccatga taacgtcctg cgtttatatg
     4381 ctgccgtttt agtttttcac agatccggtc tgcttctttt cttgtcgcag tataaatgat
     4441 gcctgcttca agcttatttc tgttcaggta ctcttctata aagcgatctt tattttcgcc
     4501 ttttgccact ttaaaggtta aattgtcacg ggaaaaccca gtataaaccg tattttcctt
     4561 ttcaatatga agctgtttgc aaatgtcctc atgcacctca ggagtagctg tcgcggttaa
     4621 tgccataatg acaggcttct cttttaactc acgaaataga atttcaatat ttctgtagct
     4681 tggcctaaaa tcgtgccccc attgcgaaat gcagtgggct tcatcaatag ctacgagcgg
     4741 gacggtgatg ctttgtaaaa tccgaatgaa ttccggtgag gttaaacgct ccggcgtaat
     4801 ataaaagaga tgataagaac cgtttttcaa agcagttaag cggctctgaa tgtcttgatt
     4861 ggattgtgtg ctgttaatat acgccgctgc gattccggct tcctcaaggg catcgacctg
     4921 atctttcatt aaggaaatca aaggagaaat cacaatcgtc gttccttcaa atagcaaagc
     4981 cggaatttga taacaaatag attttccgcc gcccgtcggc ataatacagg ccgtgttctt
     5041 tttggcttct gtcactgacc gtatcgcctg ctcctgaccg gggcgcagct tctcataccc
     5101 aaaataatgc gctaatagcg atcgggcttt ctctaacata acgtctaaga cctcgctttt
     5161 tcttcgtttc tcccatctta tcataaaaag aagccttgct attactggtc ggctgaaatt
     5221 gaattttcaa aagaaaaacg caaaataaaa agccatctcc aaaaggaaat ggctttcgct
     5281 acgtattgcc gtatgcatgt aagaaagttt taaaccacca ctcctcaaag tgggtgtttg
     5341 cagaagcgtt cctgtcgtcc tctatcttgg aggcatgaca ttccgacttc gatgtaaaac
     5401 tccctattac agcttaaagg ttaaaactta attatcaata cgaacaatgt agcttctatt
     5461 aaagaataca cgattactga cattttttca agtggttatg atattacaat aaaaaaactt
     5521 ttttcagagc tcagaaaaga cactttactg agacagctct atcacattca gttttttcat
     5581 tatataaata taataaaata gagataaatt tccataaaat aaaaaccccc ggctataaaa
     5641 agcacgggag tttccatcaa tattagaatt tagcagcaag gtcttttgct tcttgtaagc
     5701 ctttttcaac gattgtttgt gcgttatccg gagctgcatt atggccttca atcaccacgg
     5761 tatgcaaatc ttgtacgccc cagaagccaa gaactgtttt catgaagttc agagacattt
     5821 caagagcagc catcggtcct tcagagtata cgcctccgcg tgcgttaaga agtgctactt
     5881 ttttgtctcc cattaagcct tttggacctt ctggcgtata tgtgaaagta acacccgcgc
     5941 gagataggta atcaacataa gtatgaagca cagcaggcac tgtgaagttc cagagcggga
     6001 atgcaaaaat aactttatca gcttttacaa attggttcag ataaacgtca gccgttgacg
     6061 cttgtttttt ctcttcttct gacatttcaa tgcctttagc ggctttgaat gttccattaa
     6121 tcatatctct tccaaggtaa ggaaggtttt ctttatgaag gtccagttcg atcacttcat
     6181 catttggatt attttcttta taagcagcca agaaagcttc atataatttt actgaaacac
     6241 cttcttcagc tgtacgatcg cttgctttaa caaataagac ttgagacatt tttgttcctc
     6301 ccggtaatct ttttatattt acttacaaat agtatgttac tttctttttt aaataatgtc
     6361 aatccattta tttcgaattc gaggtttttt tacgaaaaaa aaggcaagta ctgcaaatgc
     6421 ctgtactcgc cttcttgaaa cttttatttt gagtcctgct cccgatctaa aacttcgtac
     6481 gtagcggagc ttttttgatc aatcgtttct ttattcgaaa ggtctttcac ctgtgcatga
     6541 aattcctttt caaccgcagg tgcatcaggc tctagagcct cttccctgtc agtctgagct
     6601 tcaacagtca tccttgcgta cggcacagca tcgagccttt cataagggat ttcttctcca
     6661 gtcgcttcac atattccata agttccgtct tttatttttt gtaaagcatt gtttacttca
     6721 tctaatagtt ccctgtctac ttcctttacc gtttggtctg tcattctgtc cgtatacagg
     6781 ctgccgtggt ctgccatatg gttatccact ccgttagaca gctcacccgc ttcttctgtc
     6841 atagactccg ttgctttttt cgggccggac aattcttttt gcagatctag caatttatga
     6901 tagagatgct gagtttgttc tttagttaat gccatgtgta accactcctt tcttcttgct
     6961 ttcccgtttt taaaaagaac aaaccttaaa acaaaaaaca ccttcgtttc tgctttttca
     7021 caaaaaaaac cggccatcat aaagacgtcc ggtgtgtccg ctaagctgtg aaacacttta
     7081 gctgcataaa tattttgtca aaatcgcctg aacttcactc aggtcggtgt ctttcgcaaa
     7141 ttctaaatcg aaggtcttgc ttcttgtcat cagcaaaagt ccagtatcgg ggtcaaacat
     7201 tcccgcttcc tgaatagcaa agctttcaat cgttttataa ggaagaaata cacgcacttt
     7261 ctttttggag aacattttat tatcaaaaaa tatgattcgt ttatttgtaa agcaaatttg
     7321 gtcaacccgg agcttgtaga tagattctat tctttcccct tcaacgagca aagcttcaag
     7381 tttctttgcg tcagttggtc tgctaacaga aaaaataccc aattaagaac ccctcttcct
     7441 gcatttatct attattttta ttatcagtac gtaaaataag ttctatatcc ctatataaga
     7501 atacgacatg gacgtcattt aaaccattac gaatagatta cattttctaa aaatccgatc
     7561 cgatcatttt aaaaaccgcg cgcttatttg ctgtcataac atagtacgtg cagatataga
     7621 tgaaagtttc atttttcagg gtcatttcca gattattttt taaaaaattt cgtttatttc
     7681 agataattcg gttttatttt ccatttatct gaacgaaaaa aaaccgcagt tctttgcctg
     7741 cggttttaag atcatctaat cccgatccgt atcttcaatc gttccttcat aatacgtatc
     7801 tgaaatttgt tcccttgtga catcctcgcc ttctgtagga tgcttttgtt ctgggaagtt
     7861 ctctgcttct tcttttccgt tattgacttg tcttttcatt atgcattcac ccttttttct
     7921 ttgatcattc tttattatgg atgatgcatg gcatattcat tcagtcaatc agacctcttc
     7981 aaagtactct ttatagaatc cgccaacttt attggaattg tctacgacaa acaggaattc
     8041 ttctgtttcg tttttcactt catattcttt ccccggcgtt aaaacgcggt ttacgatgta
     8101 tttttttgca tttgtttgaa cacattttac ttttctcact gtttcttttt tcagccatgt
     8161 tttatgaatc attctaaaag ctcctttttc ttatcgatat gttcattgta gcttttttct
     8221 cttatcaaat gcaataatgg ttattcattg attttgctat accctctatt ataaaatcat
     8281 tgtatacgct tactaatatc acaaacttcg cagtctaacc attccaaacc aacgcaccaa
     8341 cgaatcgact atcaccaaga atctcagtgc agaccattac aaattacagg aggtttgtta
     8401 caaatgagtt ctttaacgat gcaaatcagt aagaggctgg agacattttt acaagggacc
     8461 aaaaaactgt acattgacgg caaatttgtt ccgagtgctt caggagcaac ctttgtcacc
     8521 ccaaatccgg caaccggtga gacgttgatg acactcttcg aagcacaagc cgaggatgtt
     8581 gataaagctg taaaagccgc aagaaaggcc tttgatcaag gtgagtggcg cacgatgccg
     8641 gcagcttcca gaagcagatt gatgtataaa ttggctgacc tgatggagga gcatcaaaca
     8701 gaactcgctc agcttgaaac actcgacaac ggaaaaccga taagcgaaac aacaaacgga
     8761 gatattccgc tggccataga gcatatgcgc tactatgccg gctggtctac taaaataacc
     8821 ggacagacga tacctgttgc gggttcttat tttaattata ccaagcatga ccctgtcgga
     8881 gtcgttggtc aaatcatccc ttggaacttc cctcttctta tggccatgtg gaaaatgggc
     8941 gctgcgcttg cgacaggatg caccattatc ttaaagcccg ctgagcaaac gccgcttacg
     9001 gcgctatact tggctgaatt aattgatgaa gccggttttc ctgacggcgt tattaatatt
     9061 attcccggat ttggagaaac tgcgggagaa gcgctgtcca accatgagga agtggataag
     9121 atcgccttta caggttccac tgaaatcggc aaaaaaatta tggaaacagc cgcaaagtca
     9181 attaagcgcg taacattaga gcttggcgga aagtctccta atatcatctt gcctgacgcg
     9241 aacttgaaaa aagcaattcc aggagccttg aacggtgtta tgtttaacca aggccaagtg
     9301 tgctgcgctg gttcacgggt ctttattcaa aaagaccgat acgatgaagt ggtcagtgag
     9361 atggttactt atgcggaatc ccttcgtcaa ggtgcaggcc tgcacaaaga tacacaaatc
     9421 ggaccgcttg taagcaaaga acagcacgaa cgtgtccttt cctatattca aaaaggcaag
     9481 gatgaaggag cgaaagccgt gacaggcgga agctgtccgt atgaggaagg ttattttgta
     9541 tcaccaactg tatttaccaa cgtagaagac ggcatgacaa ttgcaaaaga agaaattttc
     9601 ggcccggtct taactgccat tccatacgat acggtcgatg aagtgatcga acgggcaaac
     9661 aataccgaat atggccttgc agccggattg tggaccgaaa atctgaaaca tgctcactat
     9721 atcgctgacc gcctgcaagc cggaacagtc tgggtgaatt gctataacgt gtttgatgcc
     9781 gcatccccat tcggcggtta caagcaatca ggacttggaa gagaaatggg atcatacgcc
     9841 ttaaataatt acacggaagt gaaaagcgtg tggattaact tagaggatta aaaagaagat
     9901 tttgaaaaac tgctgccctg acagcagttt ttttgttttt tcattctgta atgccgtcga
     9961 aagagcatga atatatatgc ttctgaagga gttgaacgcg atgagcacac ttcaggagca
    10021 ggtagcacgc tgccaaaaga aaaccataac acagctgaaa aacatgcaaa atgctgatgg
    10081 ttcatggtct ttttgctttg aaggcccaat aatgacgaat tcatttttca tcctccttct
    10141 cacctcattt gaagataagg gtaatgaaga actgattgta aaattggcaa aaggcatacg
    10201 ggaaaaacag cggccggatg gaacgtttac caattatccc gatgaagaaa aagggaatgt
    10261 gacagccact gtccaaggat acatcggaat gcttgcgtcc ggatgttatc tgaggtctga
    10321 ccctcatatg atacaggcag aacgttttat tatttctcag ggcggtctca aggatgttca
    10381 ttttatgaca aaatggatgc ttgcagccaa cgggctttat ccttggccgg cgctttacct
    10441 tccgctgtgt ctcttggcta ttcccccaac gtttccgctt catttttacc agttcagcac
    10501 gtacgcgcga attcattttg tgcccatggc cgtgactctt aatcaaagat tctttctgaa
    10561 aaatccaaat atcccctctc tcaaatatct cgattcgcat atgtctaaaa atcctttcac
    10621 atggctcaga acagatgcgt atgatcagag ggacctctct tctattctgg cctattggaa
    10681 acagctgctg aacgtgccgt tttctttaca tcagcttggc tttcgtacag ccaaaaccta
    10741 tatgcttgat cgtatagaaa aagacgggac cctgtacagc tatgccagcg cgaccatttt
    10801 tatgatctac agccttctgt cccttggcat gtcacgccat tcttccatca ttcagcaggc
    10861 tttgaacggg gttaaaacgt tggttacgga atgtgacggc atcccttatt tagagaattc
    10921 cacatctgct gtttgggaca ccgctttaat cagctatgcc cttcagcaaa gcggtgtgcc
    10981 tgagaaggat ttgatgataa ccgcagccgc agactttctg ttggaaagac agcataaaaa
    11041 gaaagcagat tggtctgtca aaaatccgca cgctgcaccc ggcggctggg gcttttcaaa
    11101 aatcaacacc attaaccctg attgtgatga taccgcagcc gcattgcaag cgattccgcg
    11161 cgccctgtct cctatgtctt gggatcgggg gctgaactgg ctattgtcta tgcaaaacaa
    11221 agacggagga ttctctgcct tcgaaagaaa tgtgaaccac cctcttatcc gtctgcttcc
    11281 gcttgagtcg gctgaggacg cagcggttga tccttcgaca cccgatttaa ccggacgcgt
    11341 gctccgcttc ttaggtgaaa aagcaggtat gacacaggat aacaggcaaa taaaaaaagc
    11401 cgtcaactgg ctcatcgata accaggagga taacggatca tggtacggca gatggggcgt
    11461 ttgctatatt tacggaacat gggccgctat aacaggaatg cgtgcctgcg gtacagaagc
    11521 gtttcaccct gctgtaaaaa aagcggtgca ttggctgaaa acaattcagc atgatgatgg
    11581 cggctggggt gaatcctgca gaagcgccga agtcaaaaca tacgtacctt tacgatatgg
    11641 aacggtaacc caaacagcct gggcattaga tgctttattg caatatgaag aacctgatca
    11701 cccgtctatt acaaaaggac tcgagtttct tattaacagc gaaaatcaca acaagcacag
    11761 ttttgactat ccaacaggaa tcggactgcc aaaacagttt tatattcggt atcacagcta
    11821 cccctttgcc ttttctttac tggcttattc aagctatatg aaatctattg aaaagaggga
    11881 gatgaaatga aacgccatac gtaccaaacc gagatgatca attggtgtga ggatttaaaa
    11941 gaacaggtt
//
