LOCUS       NZ_JALATJ010000002     10366 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain WR203A-1m Contig_0000002, whole genome
            shotgun sequence.
ACCESSION   NZ_JALATJ010000002
VERSION     NZ_JALATJ010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1027317)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1027317)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 488.8726095687877x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:44:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,221
            CDSs (total)                      :: 4,141
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 80 of 128
            Pseudo Genes (incomplete)         :: 71 of 128
            Pseudo Genes (internal stop)      :: 24 of 128
            Pseudo Genes (multiple problems)  :: 38 of 128
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:31:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 924880
            Orig. end                         :: 935246
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALATJ010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10366
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10366
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10366
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5366](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5366
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            775..1257
                     /locus_tag="MOD90_RS08185"
                     /old_locus_tag="MOD90_08155"
     CDS             775..1257
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258635.1"
                     /locus_tag="MOD90_RS08185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08155"
                     /product="GyrI-like domain-containing protein"
                     /protein_id="WP_060398811.1"
                     /transl_table=11
                     /translation="MSFSHITHLKQKRFAGLSQRTSNALEMTEERRIPALWDQFWQQDM
                     SILFSQAEKDQSIIALYSNYEQETNGFYTFSVGAFQEDSGTLPEPYENIDLPASAYAVF
                     TSRIGPIEEIVYETWKEIWTWDKRHLRTFTGDFEMYDQSAAVPQRAQVNIYVAIKN"
     gene            1334..2035
                     /locus_tag="MOD90_RS08190"
                     /old_locus_tag="MOD90_08160"
     CDS             1334..2035
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389789.1"
                     /locus_tag="MOD90_RS08190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08160"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_268280430.1"
                     /transl_table=11
                     /translation="MRVTQYQTVWQLTFFPVLFPVNCYLVEEENEVTLVDAAIPSSYKG
                     IIQAVNQLGKPLQHILLTHAHGDHVGSLDTLTQAFPHAKVMISERDSFLLQGDTSLRHD
                     EPQTPIKGGIPKHVQTKPHHLLTGGETIGSLLAIPTPGHTPGSMSFLDTRNGIIIAGDA
                     FQLRGGIAVSGQVKWTFPFPAFATWNKEEAIKSAQLLADKAPSCLAVGHGNFLRSPSER
                     MRQAIQKAKKG"
     gene            2040..2615
                     /locus_tag="MOD90_RS08195"
                     /old_locus_tag="MOD90_08165"
     CDS             2040..2615
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389788.1"
                     /locus_tag="MOD90_RS08195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08165"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_268280431.1"
                     /transl_table=11
                     /translation="MSPRIGLNQKMIVDAAAEIADQEGLNSVTLAALSKKMNVRPPSLY
                     NHVNGLQAIRTELAVNGLTKLFDQMAASVTEQKGDAALLYLAHAYVDFAVGNPGYYEAA
                     LLKVQDKRAEIVSDQIVQLVTNLLIENRYACEKTAIHATRGLRSLLHGFTALIAKEAFE
                     REEDISESLSFSIRTFLSGLSINNKNIM"
     gene            2693..3436
                     /locus_tag="MOD90_RS08200"
                     /old_locus_tag="MOD90_08170"
     CDS             2693..3436
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389787.1"
                     /locus_tag="MOD90_RS08200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08170"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_268280432.1"
                     /transl_table=11
                     /translation="MDDIHKIEQLAAASWPSYIQKSMGQWLLRANFGVTKRANSVWTSA
                     DMPEGEFQQEAEHFYKSLGLPVCFHISNASPKGLDGVLADRGYEKADECFQMTALCRDI
                     MNRTEDNRRFTYKWEQVPSSVWIDNFIQLEEFSPERHRGYAYIFERMPPCKTFFTMYDQ
                     GSLTAVGTASVIDGYGGLSNIVVAKEHRGKGAGTQVIRVLTEWAISNGAKHIYLQVLKE
                     NLAAVSLYEKIGFSPISEHHYRIQK"
     gene            3451..4176
                     /locus_tag="MOD90_RS08205"
                     /old_locus_tag="MOD90_08175"
     CDS             3451..4176
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389786.1"
                     /locus_tag="MOD90_RS08205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08175"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_268280487.1"
                     /transl_table=11
                     /translation="MLMDSLQEIICEKRTYTRLYHSHKHAYSQFLFPLEGSIDLETEGR
                     EVKLKPDRFLYIPPECEHRFRSIGRNECLVLDVPLYAMKIDEYHAGSGIEAALDPFWSS
                     IRYLLTEEAKAGTANSLHMLVQYIKEKLQSHTCDSIAYIHSHLFERLTIKKLAEIEHYH
                     PAYYSSWFKKQTGKSPQSYIANLRLEEAKRMLIEKKETLTNVSEALGFQNLSSFTRWFT
                     KSTGMPPRLYRNTLYSDKK"
     gene            4241..4573
                     /gene="csaA"
                     /locus_tag="MOD90_RS08210"
                     /old_locus_tag="MOD90_08180"
     CDS             4241..4573
                     /codon_start=1
                     /gene="csaA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389785.1"
                     /locus_tag="MOD90_RS08210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08180"
                     /product="chaperone CsaA"
                     /protein_id="WP_268277082.1"
                     /transl_table=11
                     /translation="MAVIEDFEKLDIRTGTIVKAEEFPEARVPAIKLLIDFGAEIGMKQ
                     SSAQITKRYQPEDLINKQIIAVINFPPRRIAGFKSEVLVLGGIPGQGDVVLLQPGQSVP
                     NGTKIG"
     misc_feature    complement(4865..4880)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            5001..5366
                     /locus_tag="MOD90_RS08215"
                     /old_locus_tag="MOD90_08185"
     CDS             5001..5366
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="MOD90_RS08215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08185"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_268280433.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 9.3e-07,
                     bitscore: 25.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKVFSSMVLGATLLVSSSVIFASSSDEATHGEVNLDETRDLIQ
                     IMKLTRTKAGGGTWYHGFEDGQVKSNYNHTKKTHKSSAKAGGERFFSSKWKKKDEGYTY
                     ASVYQTLWGNRAFWDTK"
     gene            5459..7630
                     /locus_tag="MOD90_RS08220"
                     /old_locus_tag="MOD90_08190"
     CDS             5459..7630
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="MOD90_RS08220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08190"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_268280434.1"
                     /transl_table=11
                     /translation="MKKTIYILFILFLLSMNISSYHYTLNKQVFYTLFSNKQTLIINYN
                     KAHFKSDDFIKEIAGFSKKENVNITQYNFLDEKTLNIYASNLKNEPSIKIGDRFISNFN
                     KTANESNKNLIGTIDFPLSTWKIRYFNLNQISNVGLGNRFYISSEDEDVIEQAKKLFSD
                     YGDVSIEENISLNDSAVFSTALLLLALLSMLILFIGILYFVIKNRKQLLLQKLWGYSKT
                     KSLLTFPKMFLKPLVMIIICGALLTVILAFIFNLNDWLSVYVKMYMRNALIVTFSMMVY
                     TIIVTLILYNNSNISATIKGGIPFKKFQWLSLGFKLIITILLFNIFASSLSNLFDLNQR
                     LDNQSYWNKTQTVYKTSFADTGLNYSDLKIDREKNEKVRKLYHELERHKDAFIMDAQNY
                     GILKMNGKTLVYFYTLNTTKENEIYSPSGRSVTISPNYLKVNTIKGINGKTITEKQLTF
                     DQNTLNLLVPKKYAKYKEKILKAYKNQFFFDKVEVDNMYNKEIQQPLNTLKKERLKIKI
                     IYTQNNQEYFTYNSMLGNNKNNIVDPIAIVFTDNVDSSVIGAYATSSLFFSDASKGMAY
                     DHIIPYLDKTDTKELIGSAISVYQELSDQIARIQNQFIQNLIGLTITLILSVAFLISYI
                     WSYYSANAYRLYLKELFGYSYWARNKSLIILSTVSNCLIGICFSIYYKIYELIGLIVLF
                     FGIEMIILYFLSLYLNRKNINKILKGDRL"
     misc_feature    5464..5475
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.95"
                     /tool="antismash"
     gene            7627..8247
                     /locus_tag="MOD90_RS08225"
                     /old_locus_tag="MOD90_08195"
     CDS             7627..8247
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328395.1"
                     /locus_tag="MOD90_RS08225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08195"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_268280435.1"
                     /transl_table=11
                     /translation="MIELKSISKSFDGKSVLSNFSIHIEENEFVSIVGKSGSGKTTLLN
                     IISLLDSPDEGEIEILGYKNPKSKEVMKLRRENLGYIFQNYVLMDNETVLTNLLLSTAY
                     AKDFDKNKLSEALEMVGLEKSFLKKKVYQLSGGEQQRVAIARIILKPCDIILADEPTGN
                     LDEYNKNIILSLFHQLKEMGKTIICVTHDPEIANCSDRVINLS"
     gene            8261..8473
                     /locus_tag="MOD90_RS08230"
                     /old_locus_tag="MOD90_08200"
     CDS             8261..8473
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328394.1"
                     /locus_tag="MOD90_RS08230"
                     /note="Built to rescue LMOF2365_14255 during structural
                     annotation change regression.; Built to rescue
                     LMOF2365_14255 during structural annotation change
                     regression; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08200"
                     /product="bacteriocin-like WGxF protein"
                     /protein_id="WP_268280436.1"
                     /transl_table=11
                     /translation="MKHITFAISTSILIIITGIVHRVIFRLFGFPFDEALFFWGGFVVV
                     YFVIALIASLLFRNVGSSSSRRYQA"
     gene            complement(8657..9169)
                     /locus_tag="MOD90_RS08235"
                     /old_locus_tag="MOD90_08205"
     CDS             complement(8657..9169)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MOD90_RS08235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MOD90_08205"
                     /product="hypothetical protein"
                     /protein_id="WP_268280437.1"
                     /transl_table=11
                     /translation="MKKTVLSTISLGLLFSTVAPLTSHAESISEPTPSYITQVNSTPLQ
                     ELIKTSSVPVIQENGEISIQAVPPSTKWKVVDHFRGNSKNIDTAAELGARIAAGLPGLK
                     YSWAGIFAQAVFGTFYHKKPTRYYSTTQYLAYDKKYRYTIVDMYVYKDSKYSKQIGYDY
                     KVFRTPR"
     gene            9718..10188
                     /locus_tag="MOD90_RS08240"
                     /old_locus_tag="MOD90_08210"
     CDS             9718..10188
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014479885.1"
                     /locus_tag="MOD90_RS08240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD90_08210"
                     /product="hypothetical protein"
                     /protein_id="WP_268280438.1"
                     /transl_table=11
                     /translation="MWKALILLKKQKNQSSIDEGYLQIPEKDFNLELKQMIFELESLGE
                     AVSEKLQNKYIDVFEAQGKPNLSIKVCRDIDGDVINDLTAERCFEKEYRSQITINYYQP
                     AKAYDEDIDCITMDLWYYYGGFIGSGCGTLFDLKNNDLEKEIEEVLIFLLND"
ORIGIN
        1 agaggaggag ctgtttgcga ttgcttccgc tttgcagagt gtctcttcca tgtgtgaacc
       61 tgcttctcac agcacagctt atcaaaaaat taagcacctc ataccggagc aatctgctga
      121 agcatttaag catcaaacag aaaaatggtt tattgacatg acggcttggg gccatactga
      181 ggatcaaaag gcacttcgtg agaaaatcag tgccgccatc gatcaattgt caacgatatc
      241 atttacatat acgaacgcca gcggggaaac acttctacgt gaaacagaac cctacacatt
      301 ggtttgcaag gcggggcact ggtatttata cgcctattgt ctggttagaa acgattttcg
      361 cttttttaaa cttaaccgga taaaagatat ggccatatta caaaattcct ttatcagaaa
      421 agacattcag ctggatacct tgccgtggga taggaattgg tacgaaaagg accgtttaac
      481 tgaactggtt attcttgttc agcagccagc acgtcagcgc atacgtgaat ggttcggatc
      541 tgaggttctt cattgtgatg aagatgagaa ctgccgggca gtgatttctc ttccagaaga
      601 tcaatggctg atcggttttt tgctgcagtt cgggaaggat attgaggttt tggaacccct
      661 tcatatacgg gataaagtaa aagataccat tcatcagatg caaaaagtat atgaaacctg
      721 acacactgct gtcaggtttc tttttttata atctaggtaa aaaggaggga acatatgagt
      781 ttttcacata taacacactt aaaacagaaa cggtttgccg gactatcaca gcggacaagc
      841 aacgcattgg aaatgacaga agaaaggcgg atcccggccc tatgggacca attttggcag
      901 caggatatga gcattctttt ctcacaggct gaaaaagatc aaagcattat cgccttatat
      961 tccaattacg aacaggagac gaatgggttt tacacatttt cagtcggagc gtttcaggag
     1021 gacagcggga ctcttccgga accgtatgaa aacattgatt tgccggcttc cgcttatgcc
     1081 gttttcactt ccagaatcgg ccctattgaa gagattgtgt atgaaacgtg gaaggaaatt
     1141 tggacttggg ataagcgtca tctgcgcaca tttacaggcg actttgagat gtatgatcaa
     1201 agtgcagcag tacctcaacg ggcgcaagtt aatatatatg tcgccataaa aaattgataa
     1261 actcttgaaa agctaatggt gttagttata atgaaactaa taccattagt tttttgttag
     1321 ggggaataaa aagatgagag ttacccagta tcaaacggtt tggcagctga ctttttttcc
     1381 agttttgttt ccggtcaatt gctatttagt agaggaagaa aacgaagtca cccttgttga
     1441 tgctgcaatt ccaagcagct ataaaggaat tattcaagct gtaaatcagc tgggaaaacc
     1501 gcttcagcat attttattga cccatgcaca tggggatcat gtcggatcac tggatacatt
     1561 gacacaagcg tttcctcatg caaaggtgat gatctcagaa cgggattctt ttctgctgca
     1621 aggtgataca tccttgcgtc atgatgagcc gcagacacct ataaaaggag gtattccgaa
     1681 acacgtgcaa acaaagcctc accatctgct gacgggcggt gaaacaattg gttctctgct
     1741 tgcaattcca acaccgggcc atacaccggg cagcatgtca tttcttgata caagaaacgg
     1801 cattataata gcaggagatg catttcagtt gcgcggagga attgctgtat caggccaggt
     1861 aaaatggacg tttccttttc ctgccttcgc cacatggaat aaggaggaag ccatcaaaag
     1921 cgcacaattg ctcgccgaca aggcgccttc ctgtctcgct gtcggacatg ggaattttct
     1981 gagatcaccc tcagagcgga tgcgacaggc gatacaaaaa gcaaagaaag gatgatagca
     2041 tgtcacccag aataggattg aaccaaaaaa tgattgtaga cgcggcagcg gagattgccg
     2101 atcaggaagg cttgaatagt gtcacgcttg ccgcactgtc taaaaaaatg aatgttcgcc
     2161 ctccatcgct gtataatcat gtcaacgggc tgcaagcaat cagaacagag cttgctgtca
     2221 acgggctgac aaagcttttt gatcaaatgg cagcctctgt aaccgaacaa aagggagatg
     2281 ccgctctgct ttatttagct catgcatatg ttgattttgc agttggaaat cccggatatt
     2341 atgaggcggc tttgcttaag gttcaggaca aaagggcgga aatcgtgtca gaccaaattg
     2401 tccagcttgt gacgaatctg ctcattgaaa atagatatgc atgcgaaaag actgccattc
     2461 acgcgacaag ggggctcaga agtttgcttc atggctttac tgctttaatc gcaaaagaag
     2521 catttgaacg tgaagaggat atttcagaaa gtctgtcatt ttcaattcgg acgtttttgt
     2581 caggattatc tattaacaat aaaaatatta tgtaaactaa taaatagatg tcttatattt
     2641 ctgaatattg tataatgtca tggtagtata cataggggag agacattatg ctatggatga
     2701 tatccataaa attgagcagt tagcggcagc ttcatggccg tcttatattc agaaaagcat
     2761 gggacagtgg cttctcagag ccaactttgg cgtaaccaaa agggcgaaca gcgtatggac
     2821 ttctgcggat atgcctgaag gagaattcca gcaagaggcc gagcattttt ataaaagctt
     2881 aggcttgcct gtttgttttc atatcagtaa tgcatctccg aaagggcttg atggcgtatt
     2941 ggcagacagg ggctatgaaa aggcagacga gtgttttcaa atgactgcat tgtgccggga
     3001 cattatgaac cgtacagaag acaatcgccg atttacatat aagtgggaac aggtaccgtc
     3061 ttctgtttgg attgataatt ttattcaatt ggaagaattt tcacctgagc ggcatcgagg
     3121 ttatgcatac attttcgaac gaatgccgcc gtgtaaaacc tttttcacga tgtatgacca
     3181 agggagcctg acagcggttg gaacggcttc tgtcattgat ggctatggcg gactcagtaa
     3241 catcgttgta gccaaggagc atagaggaaa aggggcggga actcaagtaa tcagggtgtt
     3301 gactgaatgg gcaatcagca acggagcgaa gcacatctat ctgcaagtgc tgaaagaaaa
     3361 ccttgcggca gtttctttat atgaaaaaat cggattttcg cctatatcgg aacaccatta
     3421 ccggatccaa aaatgaccaa aggaaggggc ttgctgatgg actcgcttca agagattatt
     3481 tgcgaaaaac ggacatatac acgcctttat cattcacata agcatgcgta cagccaattt
     3541 ctttttccgc ttgaagggag tattgatctt gagacggagg ggcgcgaggt gaaattaaag
     3601 ccggatcggt ttttatatat tccgcctgag tgcgagcatc gtttccgttc aatcggcaga
     3661 aatgaatgtc ttgtactgga tgttccgctt tatgcgatga aaattgatga gtatcatgca
     3721 ggatcgggaa ttgaagctgc acttgatcct ttctggtcat caatcaggta tttgctgacg
     3781 gaggaagcaa aggccggcac agcaaactcg ctgcatatgc ttgttcaata cataaaagaa
     3841 aagctgcaat cccatacctg cgactcaatt gcctacattc acagccattt atttgagcgg
     3901 ctgacgatta agaagctggc agaaatcgag cattaccacc cagcctacta ttcaagctgg
     3961 ttcaagaagc agaccgggaa gtctccgcag agctatattg ccaatcttcg tctcgaggaa
     4021 gcgaaacgaa tgctgataga gaaaaaggaa acccttacta acgtgagcga ggcgctgggc
     4081 tttcaaaatc tatcttcatt tacaagatgg ttcacgaaaa gtacaggcat gccgccccgg
     4141 ctatatcgaa ataccttata ttcggataaa aaatgatggt cctttggtta aacaaacctc
     4201 atacacctct tttataatag gagaaaaagg agttattgat atggcagtca ttgaagactt
     4261 tgagaaattg gatatcagga caggcactat tgtaaaagcg gaggaatttc ctgaagcaag
     4321 agtcccggcc attaagcttt tgattgattt tggagcagaa atcggcatga aacaatcgag
     4381 cgcgcaaatc acgaagcgtt atcagccgga agatctcatc aacaagcaaa tcatagcagt
     4441 catcaacttt ccaccgcgcc ggatcgccgg atttaaatcg gaagttttgg tcctcggggg
     4501 cattcccggc cagggcgatg tcgtcctgct gcagccggga caatccgtcc caaacggcac
     4561 aaaaatcgga taacataaaa aagacgtttg ccaaaggcaa acgtctttgc gtattgaggc
     4621 gatgcaccac acgccacatt cgtgtgtgcc gcaaagggca tgtacatcgg cttacacaat
     4681 tcagctcatc gctatatgaa tatagcatgg cggctgaagg aatgcaaggg taacttttta
     4741 attgtgagtg aaatagttat aaaagcttgt cttattttgt ctaaattaat cgtgagtaat
     4801 tcaaatatta ctgtatgatg atgattgttc gacaataatg agtttgtcaa caataacacg
     4861 gaatgtgact taactgtgac ctcaaacacc attttaagaa taggcagaaa tgcctttaaa
     4921 caaacgaaaa cgtacttaat ggtgaataca aggggtaact gtgacctggt ttcttgtgaa
     4981 aacaaaaagg agaggttata ttgaaaaaga aagttttttc ttcaatggtg ttgggagcta
     5041 ctttactagt atcatcttct gtcatttttg ctagttcttc agatgaggct acacacggag
     5101 aagtgaactt ggatgaaaca agggatttaa tacaaatcat gaaattgacc agaaccaaag
     5161 cgggtggcgg tacttggtat cacggtttcg aagatgggca agttaaatcc aattacaatc
     5221 atactaaaaa gactcacaaa tcaagtgcga aggctggtgg agaaaggttc ttttcatcta
     5281 aatggaagaa aaaagatgaa gggtatactt acgcttcagt gtatcaaact ctttggggaa
     5341 atagggcttt ttgggatacg aaataaaaca gacaataaca aagtgatttt taatctactt
     5401 ccggtgccgt caaaacttga atataaggca ccggatttct ttttatggag gctatccaat
     5461 gaagaaaacg atttatatat tgttcattct atttttactc tcaatgaaca tatccagcta
     5521 tcactatact ctgaataagc aagtttttta tactttattc tctaataaac aaacgttaat
     5581 tattaattac aacaaagcac actttaaaag tgatgatttc attaaggaaa tagctggatt
     5641 ttcaaaaaaa gagaacgtga atattactca atacaatttt ttagacgaga agaccttaaa
     5701 tatctacgct tcaaacctta agaatgaacc ttcaatcaaa ataggagata ggtttatatc
     5761 taatttcaat aaaacagcaa atgaatcaaa taaaaatttg attggtacta ttgattttcc
     5821 tttatcaaca tggaaaatca gatacttcaa tttaaaccaa ataagcaacg tagggttagg
     5881 aaatagattt tatatttcat ccgaggacga agatgtaatt gaacaagcta aaaagctatt
     5941 ctcagactat ggtgatgtca gtattgaaga aaacatcagt ttaaatgact ctgctgtgtt
     6001 cagcaccgca ttgctcttgt tagctttgct ttccatgcta atcttattta ttggcatcct
     6061 ttatttcgta attaaaaatc gcaagcaact tctcttacag aagctatggg gatattcgaa
     6121 aaccaaatca ctgttaactt tcccaaaaat gtttttaaaa ccacttgtta tgatcataat
     6181 ttgcggcgcc cttctcactg taattctggc ttttatattc aacctaaacg actggttatc
     6241 agtgtacgtt aaaatgtaca tgcgaaatgc actgattgtg acattctcaa tgatggtata
     6301 cacaattatt gttactttga ttttatataa caacagcaat ataagcgcca cgataaaagg
     6361 cggtatacca tttaaaaagt ttcaatggct gtcattgggt tttaagttaa ttataacaat
     6421 cctacttttt aatatttttg cttcatccct ctctaatttg ttcgatttaa accaacggct
     6481 agacaatcaa tcttattgga ataagacaca aacagtttat aaaaccagtt ttgctgatac
     6541 aggtttaaac tacagtgact taaaaattga tagagaaaaa aacgagaagg tacgaaagct
     6601 ttatcatgaa ttagaaagac ataaagatgc gtttataatg gatgctcaaa actatggaat
     6661 actgaaaatg aatgggaaaa ctctagttta tttctataca ttgaatacaa ccaaagaaaa
     6721 tgaaatttat tcaccatccg gaagaagcgt aacgattagt cctaattatt taaaagtaaa
     6781 caccattaaa ggaattaacg gaaaaacgat tactgaaaaa caattaacct tcgatcaaaa
     6841 cacattgaat ttacttgttc caaaaaaata cgctaagtat aaagaaaaaa tcctcaaagc
     6901 ttacaaaaat caatttttct ttgataaggt tgaagtagac aatatgtata acaaagaaat
     6961 tcagcaacca ttgaatacgc tgaaaaaaga gcggttgaaa atcaaaataa tatatactca
     7021 aaataatcaa gaatacttca cttataatag tatgctcggc aacaataaaa acaatattgt
     7081 tgatccaatt gcaattgtat tcactgataa tgttgattca tcagttatag gtgcatatgc
     7141 aacctcaagt ttattctttt ctgatgcctc aaaaggtatg gcatatgacc acatcatccc
     7201 ttatttagat aaaacagata caaaagagct gattggttct gcgatatcgg tctatcaaga
     7261 attaagcgac caaatagcac gaattcaaaa tcagttcatc cagaatctaa ttggattaac
     7321 tataacactc atattgtcag tagccttcct tatctcttat atatggtcat actacagcgc
     7381 aaatgcttat cgactttacc ttaaagagtt atttggatat tcatattggg ctagaaataa
     7441 gagcttaatc atattatcga ctgtgtctaa ctgcctgata ggaatctgct ttagtattta
     7501 ttataaaatc tatgaattaa taggcttgat tgtattgttt tttggaattg aaatgattat
     7561 tctgtatttc ctcagtcttt atttaaacag gaaaaacata aataagattt taaaaggaga
     7621 cagattatga tagaacttaa aagcatatcc aaaagttttg atggaaaatc agttctttct
     7681 aacttttcaa ttcacataga ggaaaatgaa ttcgtttcta ttgttggaaa gagcggttct
     7741 ggcaaaacca ctcttctaaa tataatcagc cttttagatt ctccagatga aggcgaaatt
     7801 gagattttag gatataaaaa tcctaaatct aaagaggtga tgaagttaag gagagagaat
     7861 ttaggatata tctttcaaaa ctatgtgctc atggataatg aaactgtatt aacaaattta
     7921 ttgctttcca cagcatacgc aaaagacttt gataagaaca agctgtcaga agctctagag
     7981 atggtcggct tagaaaaaag ctttttaaag aaaaaggtat accagttgag cggtggtgag
     8041 caacagcgtg tagcaatagc caggattatt ctaaaaccct gcgatatcat tctggccgat
     8101 gaaccaaccg gaaaccttga tgagtacaac aagaacatta ttctttctct tttccatcag
     8161 ttgaaagaaa tggggaaaac aatcatatgt gtaacacatg atccagaaat agctaactgc
     8221 tctgatagag taataaatct ttcgtaagga gttgttcttg atgaaacata tcacttttgc
     8281 aatttctaca agcatcctga ttattattac cggtattgtt cacagggtta tttttagact
     8341 atttggattt ccattcgatg aagcgctttt cttctggggc ggatttgttg ttgtttattt
     8401 tgttatagct ttgattgctt ccttactctt tagaaatgtt ggttcttcta gcagcaggag
     8461 atatcaggcg taactcaatc tggtcattaa acaataaaat ataccttatc ttgaatacat
     8521 cccaaatata aagtatcctg tatatgtatc aaagttggtg tatcaaccac tataggccag
     8581 agagttccct cacatactct ctggcttttt gtttaaaaag ttaaaagccc tttaaaaggg
     8641 ctttatatct gacatattat ctaggagttc tgaaaacttt ataatcataa cctatttgtt
     8701 tgctatattt actgtctttg tagacgtaca tatcaacgat tgtgtatcta tactttttgt
     8761 cgtaggctaa gtattgggtt gttgagtaat aacgtgttgg ttttttatga tagaatgttc
     8821 caaacacagc ttgagcgaat ataccagccc aggagtattt aagaccaggt aaccctgcag
     8881 caattcttgc tcctaattca gctgcagtgt ctatgttctt agagttccct ctaaaatgat
     8941 ctacaacctt ccattttgtt gatggtggca cagcttgaat tgaaatttct ccattctctt
     9001 gaatgactgg aactgatgat gttttaatta attcttgaag tggtgttgaa ttgacctgag
     9061 tgatataact tggtgtaggt tctgagatgg attctgcatg gctagttagt ggagccactg
     9121 ttgagaatag gagtccgagt gatatggtag atagaactgt ttttttcact tcacaatcat
     9181 cctttcaagt tttttaatgc ttatgactat ctggatgctg tataattata acatataata
     9241 tacatatttt ggaggttgaa attatgaact ttgatgacaa aaagagtata ttaaagactg
     9301 tagggaacat atttatggtt cttgttctta tctctttatt tgtttatgca ttctccaatg
     9361 aatctattgg atggtatgtt aagatcactt ctattgtgat gttaactatc tcttatattt
     9421 gctacgcttt aatttctttg caggaaaaat ggttcatttt aactttgatt atctcctggc
     9481 ttgtgattat tttttgggta ttctaaatca ataaggctgc cgttcatact ttcgtaataa
     9541 aaaggaaggt aaacagcagc catcttttat tattgcggga aacttgtgtt ctaacagcag
     9601 gagatgcgga gtatcagcca caaattatac accccgatta ttgtatatgt actagctagt
     9661 gtaactttag gtcatagggt tccctttttt tgctttaatt aataggagga caacatagtg
     9721 tggaaagctt taatattact caagaaacag aaaaatcaaa gttcaatcga tgaaggctat
     9781 ctacagattc cggaaaagga ttttaatcta gaattaaaac aaatgatatt tgagctggag
     9841 agcctgggag aagcagtatc cgaaaagctg caaaataagt atatagatgt ttttgaagct
     9901 cagggtaaac caaatcttag catcaaagtt tgtagagaca tagatggaga tgtaataaat
     9961 gatttaacag ctgagagatg ctttgaaaaa gaataccgtt cacaaataac aattaattat
    10021 tatcaacctg caaaagccta tgatgaagat atagactgca taacaatgga tttgtggtat
    10081 tactacggtg gctttatagg aagcggttgt ggaacattat ttgatttaaa aaataatgac
    10141 ttagagaaag aaattgaaga ggttctaata tttttactca atgattaaca aatataaaaa
    10201 agaaagccac aaagggcttt caattaacgt tcacatgcat agttgtcatg atccctgtcc
    10261 attttagatt gataagcagg gtgggagcta ggcactccat ttggatattt ctttcttaac
    10321 tctgtacaat ttttaaatgt ttctgtttct ccggttgact ctgaag
//
