LOCUS       NZ_JALATG010000022      8481 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain WR2FB-1 Contig_0000022, whole genome
            shotgun sequence.
ACCESSION   NZ_JALATG010000022
VERSION     NZ_JALATG010000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 230.56309852060724x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:26:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,200
            CDSs (total)                      :: 4,134
            Genes (coding)                    :: 4,002
            CDSs (with protein)               :: 4,002
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 76 of 132
            Pseudo Genes (incomplete)         :: 74 of 132
            Pseudo Genes (internal stop)      :: 34 of 132
            Pseudo Genes (multiple problems)  :: 39 of 132
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:52:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8481
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALATG010000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..8481
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3220](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3220)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..3220)
                     /locus_tag="MOE16_RS20060"
                     /old_locus_tag="MOE16_20010"
     CDS             complement(<1..3220)
                     /NRPS_PKS="Domain: Condensation_Starter (3-300). E-value:
                     2.7e-90. Score: 294.7. Matches aSDomain:
                     nrpspksdomains_MOE16_RS20060_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (463-864). E-value:
                     2.6e-112. Score: 367.4. Matches aSDomain:
                     nrpspksdomains_MOE16_RS20060_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (966-1035). E-value: 2.1e-26. Score:
                     83.9. Matches aSDomain: nrpspksdomains_MOE16_RS20060_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOE16_RS20060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE16_20010"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268273112.1"
                     /sec_met_domain="Condensation (E-value: 4e-76, bitscore:
                     244.3, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.4e-124, bitscore:
                     403.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2e-17, bitscore:
                     52.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQHTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVL
                     EGALNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQA
                     RIPFKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDP
                     LPDQPEPSYLSYIEKESQYLQSSRFAKDRLFWTQTFQNPPEYHSLAGQTSLQKQSTSAS
                     RDTITLSPHLGQKIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKA
                     EKEMLGMFVSSLPIRITVDPDADFLSFVRRVGREQLSVMRHQRFPYNLLVNELRKEQKD
                     LHNLIGISMQYQPLQWHNADGFDYETALYFSGYTANELSVQIQERTDTGTIQLNFDYQN
                     TLFSLDDIKRIQNHLLTLLENALQHPHSFIKELDMTNIREKQKLLYEFNKTEAVSPEAP
                     TLHGLFERQAAFTPERPAIRFSSGSLTYAELDMYASRLAARLAARGVTKESIVGVLSER
                     SPEMLIAVLAVLKAGGAYLPLDPAYPEERLSYMLKDSGAALLLAQPGCSAPSFSGETLE
                     VDMASLASEEAESHVFAPADSGSLAYVIYTSGSTGQPKGVAVEHRQAVSFLTGMQRQFP
                     LQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAIHQEKVTTAH
                     FIPAMLNSFLDQAEMKALGDGTSLKRVFAGGEPLAPHVAARFASLLPQVSLIHGYGPTE
                     ATVDAAFYVLDPERDRDRLRIPIGNPVPGARLYVLDAHLAVQPCGVAGELYIAGAGVAR
                     GYLNRPALTEERFLEDPFYPGERMYKTGDLARWLPDGHVEFLGRLDDQVKIRGYRIEPG
                     EIEAALRSIEGVREAAVTVRTDSGEPELCAYTEGLRRNEVRTQLERLLPSYMIPAHMIE
                     MERWPVTPSGKLDRNALPAPGGAADAETYTAPSNVTEMKLAQLWEDVLKNGPVGIHDNF
                     FDRGGHSLKATALVSRIAKEFDVQVPLKDVFAHPTVEGLASVIREGTDSPYEAMKPTEQ
                     REAYPVSSAQKRIYVLQQLEDGGTG"
     source          1..8481
                     /db_xref="taxon:483913"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="WR2FB-1"
                     /submitter_seqid="Contig_0000022"
     region          1..8481
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..8481
                     /SMILES="NC(CCC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(32..67)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0010"
                     /evalue="3.30E+00"
                     /label="C1_starter_004-017"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="1063"
                     /protein_start="1051"
                     /score="1.7"
                     /tool="antismash"
                     /translation="YPVSSAQKRIYV"
     aSDomain        complement(116..322)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE16_RS20060_PCP.1"
                     /evalue="2.10E-26"
                     /label="MOE16_RS20060_PCP.1"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="1035"
                     /protein_start="966"
                     /score="83.9"
                     /tool="antismash"
                     /translation="EMKLAQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFD
                     VQVPLKDVFAHPTVEGLASVIREG"
     aSModule        complement(116..3211)
                     /complete
                     /domains="nrpspksdomains_MOE16_RS20060_Condensation_Starter
                     .1"
                     /domains="nrpspksdomains_MOE16_RS20060_AMP-binding.1"
                     /domains="nrpspksdomains_MOE16_RS20060_PCP.1"
                     /locus_tags="MOE16_RS20060"
                     /monomer_pairings="Glu -> Glu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(599..664)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0009"
                     /evalue="5.60E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="874"
                     /protein_start="852"
                     /score="40.7"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
     aSDomain        complement(629..1831)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE16_RS20060_AMP-binding.1"
                     /evalue="2.60E-112"
                     /label="MOE16_RS20060_AMP-binding.1"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="864"
                     /protein_start="463"
                     /score="367.4"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="FERQAAFTPERPAIRFSSGSLTYAELDMYASRLAARLAARGVTKE
                     SIVGVLSERSPEMLIAVLAVLKAGGAYLPLDPAYPEERLSYMLKDSGAALLLAQPGCSA
                     PSFSGETLEVDMASLASEEAESHVFAPADSGSLAYVIYTSGSTGQPKGVAVEHRQAVSF
                     LTGMQRQFPLQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAI
                     HQEKVTTAHFIPAMLNSFLDQAEMKALGDGTSLKRVFAGGEPLAPHVAARFASLLPQVS
                     LIHGYGPTEATVDAAFYVLDPERDRDRLRIPIGNPVPGARLYVLDAHLAVQPCGVAGEL
                     YIAGAGVARGYLNRPALTEERFLEDPFYPGERMYKTGDLARWLPDGHVEFLGRLDDQVK
                     IR"
     CDS_motif       complement(746..835)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0008"
                     /evalue="8.20E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="825"
                     /protein_start="795"
                     /score="56.5"
                     /tool="antismash"
                     /translation="PCGVAGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       complement(1361..1420)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0007"
                     /evalue="4.60E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="620"
                     /protein_start="600"
                     /score="34.4"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGQPKGVAVEH"
     CDS_motif       complement(1604..1645)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0006"
                     /evalue="6.70E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="539"
                     /protein_start="525"
                     /score="19.1"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPLD"
     CDS_motif       complement(2168..2281)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0005"
                     /evalue="2.20E-09"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="351"
                     /protein_start="313"
                     /score="29.9"
                     /tool="antismash"
                     /translation="REQLSVMRHQRFPYNLLVNELRKEQKDLHNLIGISMQY"
     aSDomain        complement(2321..3211)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE16_RS20060_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="2.70E-90"
                     /label="MOE16_RS20060_Condensation_Starter.1"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="300"
                     /protein_start="3"
                     /score="294.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="HTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVLEGA
                     LNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQARIP
                     FKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDPLPD
                     QPEPSYLSYIEKESQYLQSSRFAKDRLFWTQTFQNPPEYHSLAGQTSLQKQSTSASRDT
                     ITLSPHLGQKIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKAEKE
                     MLGMFVSSLPIRITVD"
     CDS_motif       complement(2333..2422)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0004"
                     /evalue="1.40E-08"
                     /label="C5_starter_265-294"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="296"
                     /protein_start="266"
                     /score="27.1"
                     /tool="antismash"
                     /translation="DLAIGTYYGNRGSKAEKEMLGMFVSSLPIR"
     CDS_motif       complement(2756..2821)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0003"
                     /evalue="1.10E-07"
                     /label="C3_starter_134-155"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="155"
                     /protein_start="133"
                     /score="24.7"
                     /tool="antismash"
                     /translation="FHHIIMDGISLNVMGNQIIDLY"
     CDS_motif       complement(3026..3142)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0002"
                     /evalue="7.80E-10"
                     /label="C2_starter_024-063"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="65"
                     /protein_start="26"
                     /score="31.4"
                     /tool="antismash"
                     /translation="ICNLTACVKFRGNIDLDVLEGALNHSISRNDAIRFQLLE"
     CDS_motif       complement(3167..3205)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE16_RS20060_0001"
                     /evalue="6.70E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="MOE16_RS20060"
                     /protein_end="18"
                     /protein_start="5"
                     /score="9.5"
                     /tool="antismash"
                     /translation="YSLTHAQRRVWFT"
     misc_feature    complement(3260..3276)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 22.38"
                     /tool="antismash"
     gene            complement(3591..5069)
                     /gene="dacB"
                     /locus_tag="MOE16_RS20065"
                     /old_locus_tag="MOE16_20015"
     CDS             complement(3591..5069)
                     /EC_number="3.4.16.4"
                     /GO_function="GO:0004175 - endopeptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241941.1"
                     /locus_tag="MOE16_RS20065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE16_20015"
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="WP_268273113.1"
                     /transl_table=11
                     /translation="MKKSIKLYVAVLLLFVVASVPYMHQAAQAAEKQGALAGQIDKILA
                     DHPALEGAMAGITVRSAETGAVLYEHSGEIRMRPASSLKLLTAASSLSVLGEDYSFTTE
                     VRTDGKFKGKKLNGNLYLIGKGDPTLLPSDFEEMAEKLKQSGVKEIKGNLIGDDTWYDD
                     IRLSPDMPWSDEYTYYGAQISALTASPNEDYDAGTVIVEITPGKKEGEKPAVSVSPKTD
                     YVTIKNDAETTAAGQKKDLTIEREHGTNTITIEGSVPVDASKTKEWIAVWEPAGYALDL
                     FKQALKKQGITVKGHVKTGAAPGSSDVLISHRSMPLSKLFVPFMKLSNNGHAEVLVKEM
                     GKVKKEEGSWEKGLEVLNNALPEFGVDPKSLVLRDGSGVSHINAVSSDQISQLLYDIQD
                     EKWFSAYLNSLPVAGNSDRMIGGTLRNRMKDTPAQGKVRAKTGSLSTVSSLSGYAETKS
                     GKTLVFSILLNGLIDDEDGKDIEDQIAVILANQS"
     gene            complement(5104..6081)
                     /gene="galM"
                     /locus_tag="MOE16_RS20070"
                     /old_locus_tag="MOE16_20020"
     CDS             complement(5104..6081)
                     /EC_number="5.1.3.3"
                     /codon_start=1
                     /gene="galM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389718.1"
                     /locus_tag="MOE16_RS20070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE16_20020"
                     /product="aldose 1-epimerase"
                     /protein_id="WP_060398739.1"
                     /transl_table=11
                     /translation="MANDIEKITYLGTPAIKAGNEHIEMIVVPEWGSNVISLVDKTTQL
                     QLLREPETAESFHDTPTLYGIPILFPPNRISDGTFSFRGRTYHFDINEKDKHNHLHGFL
                     YQEKWNVVTMKQTDEEVIVETEIDLSELPHVYKQFPHHAVVRMTYTLKGNTLFKHATVM
                     NNGKEAFPWGIGYHTTFVFPEESSLFSLTADQQWELDERLLPTGKLMDVPNKEALHEGM
                     DLRSQQLDDVFLSSYQKRGGENQAVIYHQHAHISIIYKADEQFKHWVVYNADGKQGYLC
                     PEPYTWVTNAVNLNVSPSLTGVQVLEPGEETTAKSSITIELNHQ"
     gene            complement(6214..7605)
                     /locus_tag="MOE16_RS20075"
                     /old_locus_tag="MOE16_20025"
     CDS             complement(6214..7605)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389719.2"
                     /locus_tag="MOE16_RS20075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE16_20025"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_060398740.1"
                     /transl_table=11
                     /translation="METSQEQLLTQKKSNPSVWRSMSLFLVPLLLSNVLQSVGQLVGMM
                     AVGRWLGVDAVAAVSSFFPLFFLLISFTIGIGSGSSILIGQAYGAKNEERLKAVVGTTL
                     TFTFLLGVVLAVIGSIFTLDILRLMGTPENVIHVSASYARILFYAMPFMFLYFAYTTFL
                     RGTGDSKTPFYTLIVSTVINIALLPVLILGMFGFPKLGIYGSAYATVISTIATFIVLMV
                     YLRKRNHPLQFDKTVRRYLKMDKELLALLLRLGIPSSINMILVSLSEIAVISFVNHYGS
                     SATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANEFGRLKQVIRVGIWLNYIIGG
                     VLIILIYVFSHQILGLFLTEQETLYIAHRLLMITLWSYLLFGNAQIISATMRASGTVLW
                     PTVISIFAIWGVEVPVAFVLSHYTNLEILGVWVGYPAAFAVSLLLIYGYYQFVWKKKQI
                     TRLIQ"
     gene            7892..8437
                     /gene="iseA"
                     /locus_tag="MOE16_RS20080"
                     /old_locus_tag="MOE16_20030"
     CDS             7892..8437
                     /codon_start=1
                     /gene="iseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389720.2"
                     /locus_tag="MOE16_RS20080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE16_20030"
                     /product="DL-endopeptidase inhibitor IseA"
                     /protein_id="WP_268273114.1"
                     /transl_table=11
                     /translation="MKKCFLFLTTIALILSLSTNAFAKNTSGDLSQKQALQLALSAREH
                     FWNTMSGHNPKAKKAVCPSGTFEYQSLQYVYMCSDLGTKAKAVNYLTPIFTKTAIEKGF
                     KDYHFTVSKGKLAVPIGDGDNLLNWKKSTAKLISKKGGTVTYEFTVPTLDGSPAAKRKV
                     TFVKENKKWKVNQFDAVI"
ORIGIN
        1 agcctgtccc tccgtcctcc agctgctgaa ggacatagat ccgtttttgc gcggaggaaa
       61 ccggataggc ctcacgctgt tccgtcggtt tcatcgcttc atatggactg tccgttcctt
      121 cacggataac ggaagccagt ccttctaccg tcggatgagc aaacacatct ttcagcggta
      181 cctgtacatc gaattctttg gcgatccggg agacaagcgc cgttgctttt aaggaatgtc
      241 ctccgcggtc gaaaaagttg tcgtgaatcc cgacaggacc atttttcagc acgtcctccc
      301 acagctgggc aagcttcatc tctgtcacat tgctcggcgc cgtgtaggtc tccgcatctg
      361 cagctccgcc aggagctggc agggcgtttc ggtcaagctt tccgcttggc gtaaccggcc
      421 accgttccat ctctatcata tgagctggga tcatgtagct cggcagcagt ctttcaagct
      481 gcgtccgcac ttcgtttctt cttagtcctt ctgtataggc gcataattcc ggctcgccgc
      541 tgtctgtccg tacggtaacg gctgcctctc tgacgccttc aatgcttcta agcgctgctt
      601 caatttctcc aggctcaatc cggtagcccc tgattttgac ttgatcatcc aaacggccga
      661 ggaattcaac atgtccgtca ggaagccagc gcgccaaatc cccggttttg tacatgcgtt
      721 cacccgggta aaacgggtcc tcaagaaaac gctcctccgt taaggccggc cgattcaaat
      781 agcctctggc cacacccgct ccggctatgt acagttcgcc ggcgacaccg caaggctgta
      841 cggctaaatg cgcatctaaa acatacaaac gcgcaccggg tacgggattc ccgatcggaa
      901 tccgcaagcg atccctgtcc cgctctgggt ccaacacgta aaatgccgcg tcaaccgtcg
      961 cttctgtcgg cccgtagcca tgaatcagcg atacctgcgg caataaagaa gcaaaacggg
     1021 ctgccacatg gggcgcaagt ggttcacctc cggcgaatac acgcttcagg cttgtcccat
     1081 caccgagcgc tttcatttcc gcttgatcga gaaagctgtt cagcatagcc ggaataaaat
     1141 gagccgttgt cactttttcc tgatgaatgg cttttacaat caatgccggg tctttctccc
     1201 agccaggcgg aagcagataa gccgaagcgc cggaaaggga ccaccaaaac agctgccaca
     1261 cagacgcatc aaaggaaaaa gaggttttca ccatgacaat gtcatcttcc tgaagcggaa
     1321 actgacgctg catgccagtc agaaaggaaa cagcctggcg gtgctcaacc gctacccctt
     1381 ttggctgccc ggtggagccc gacgtataga tgacataggc gagagagccg ctgtcagcag
     1441 gagcaaacac atggctttct gcttcttcgc tcgcaaggga tgccatgtcc acttcaagcg
     1501 tctccccgga aaagctcggt gcggaacatc ccggctgtgc cagcaagagc gcggccccgc
     1561 tgtctttcag catgtagctc agccgctcct ctggatatgc aggatcaagc ggcaaatacg
     1621 ccccgcctgc cttcaagaca gcaagtacag ctatcagcat ttcaggtgac cgttcagaca
     1681 ggacaccgac gatgctttcc ttcgtgacgc cgcgtgccgc gaggcgcgca gccagccggc
     1741 tggcatacat gtcaagctca gcgtacgtca gcgaaccgct ggaaaagcgg atggctggcc
     1801 gctccggtgt gaaagccgcc tgtcgttcaa acagtccgtg cagggtcggg gcttcaggcg
     1861 aaacagcttc cgtcttatta aactcataca acaatttttg tttctctctt atattagtca
     1921 tatctaattc ttttatgaaa ctgtgcggat gttgaagggc gttttccagt aacgtaagca
     1981 gatggttctg tattcgctta atatcatcaa gagaaaacaa ggtattttga tagtcgaaat
     2041 ttaattgaat agttcctgtg tctgtccgtt cctgtatttg aacagaaagc tcgttcgcgg
     2101 tgtatccgct gaaataaagc gccgtttcat agtcaaaacc gtctgcattg tgccattgga
     2161 gcggttgata ctgcatagat ataccgataa ggttgtgtaa atccttctgt tcttttctta
     2221 attcatttac cagcaaatta taaggaaacc gttggtgacg cataacggaa agctgttccc
     2281 tgcctactct gcggacaaag gatagaaagt ccgcgtcagg atcaaccgtt attctgatgg
     2341 gaagagaact gacgaacatg ccgagcattt ctttttcagc ctttgaccct ctgttgccat
     2401 agtaggtgcc gatggcaagg tcttttttag aagtgatccg gctgatgcat atgtaaaatg
     2461 aagccataaa taaggaaatg atattgatct tttgttcttc gcaaaagatt cgaatctttt
     2521 gccctagatg aggtgaaagg gtgatggtat ctctggatgc ggatgtgctc tgtttttgta
     2581 aagacgtctg gccggctaac gagtggtatt ccggcggatt ctgaaaggtt tgagtccaaa
     2641 ataaacggtc ctttgcaaaa cgtgaagact gtaaatattg actctctttt tcgatgtagc
     2701 tcaagtaaga aggttcaggc tgatcgggta aaggatcttg ctttttcatt ttttgataaa
     2761 gatctataat ttgatttccc atcacgttta aggagatgcc gtccattata atatgatgga
     2821 atttagcaaa cagccaaacc tcatgattgt ggattctaag gagataaaat tgataaagag
     2881 gcgagttgaa tagtttaaat gggattctcg cttgttcttg tatccattgc tctatttcta
     2941 tcatttcaac atttgaaaaa tctattattc ccagtgaagt atatttatat tcagtaaggt
     3001 acaatcgcgg ctcaagctct tctccttcta gcagctgaaa cctgatggcg tcgttgcggg
     3061 agatggaatg attcagcgcg ccctctagaa catcaagatc aatattgcct ctgaatttca
     3121 cacaggccgt aagattgcaa atgctggtac ctggctccag cagttcggta aaccacactc
     3181 tcctttgggc atgggttaaa gaataagtat gttggctcaa cggattccct ccagttctca
     3241 taataagagg ataagaaaaa agtagaaaaa tgacaataaa agaaattata tgactatata
     3301 atccatatat gcactatatt aacggatatt ttttaaagta caataaaaaa tattaaattt
     3361 ttataacatt tttttgaata tagtattatt tctttagttt tggtttcttt cttcatcaaa
     3421 tttttgaaca taaagtgata aaagtatcat ttcgattcgg gtcttacaca ctaatttttc
     3481 aattgtgcta atttgctgtt gcgcgtaatc tttctatttg ttggaagatg cgaccggaat
     3541 attattgaag acataaaaaa acctgttttc cttggaaaac aggtctgacg ctatgattga
     3601 tttgccaaga tgacagcgat ttgatcctcg atatcttttc catcctcgtc atcaatcaat
     3661 ccgttcaaaa gaatagaaaa aacaagggtt tttccactct tggtttctgc gtacccggat
     3721 aaagagctga ctgtgcttaa cgatccggtt tttgccctta cctttccttg tgcaggagtg
     3781 tctttcatcc ggttcctcag tgttccgcct atcatcctgt cactattgcc cgcaaccggc
     3841 agagaattta gataagctga gaaccatttc tcatcttgaa tgtcatataa cagctgtgag
     3901 atttgatctg aggatacagc attaatatgg gagacacctg atccgtctct taagacaagt
     3961 gatttgggat caacaccaaa ttccggaagc gcattgttta atacttcaag tcccttttcc
     4021 cagcttcctt cttctttttt cactttcccc atctctttta cgagcacttc ggcatgtccg
     4081 ttattgctta atttcataaa cggcacaaat agttttgaca gaggcattga gcgatgagag
     4141 attaacacgt ctgaagaacc gggcgcagcg cctgtcttga cgtgcccctt tacagtaatt
     4201 ccttgttttt ttagtgcctg cttaaataaa tccaaagcgt atccagccgg ctcccagaca
     4261 gcaatccatt cctttgtctt gcttgcgtca accggtacgc tcccttcaat tgtgatcgtg
     4321 tttgttccat gctctctttc gatcgtgagg tctttctttt ggccagccgc agtcgtttcg
     4381 gcatcgttct taatcgttac ataatccgtc ttcggagata cagaaacggc aggcttttcc
     4441 ccctcttttt tacccggggt gatctctaca atcacagtgc ctgcatcata atcttcattc
     4501 ggagacgctg ttaaagcgga gatttgcgcg ccgtaatatg tgtattcatc actccacggc
     4561 atatcaggcg ataatcggat gtcatcatac cacgtatcat caccgatcag attgcctttg
     4621 atctctttca cgccggattg tttcaatttc tcagccattt cttcaaaatc agatggtaaa
     4681 agtgtcggat ctcctttccc tatcaaataa aggtttccat tcagcttttt ccctttaaat
     4741 ttgccgtccg tccggacctc ggttgtaaag gaatagtcct cgccaagaac agaaagtgat
     4801 gaggccgctg tcaaaagttt taaagaagag gcgggcctca ttcggatctc tcctgaatgc
     4861 tcatatagaa cagcgcctgt ttccgcagaa cgaacagtga tgccggccat tgcgccttcg
     4921 agtgcaggat gatcagctaa aatcttatcg atctgcccgg caagtgcacc ttgtttttca
     4981 gccgcttgcg cagcctgatg catataaggt accgaagcaa cgacgaaaag cagcaaaaca
     5041 gcaacataaa gctttatgct ttttttcatt tttttacccc gcctctctgc gcctcatcat
     5101 gggctattga tgattcagtt caatggtgat actgcttttg gctgtcgttt cttctcctgg
     5161 ctccagcact tgtacaccag tgagtgatgg agacacatta agattcacag cgtttgtcac
     5221 ccatgtgtaa ggttcagggc ataaatatcc ctgctttccg tccgcgttgt atacaaccca
     5281 atgtttaaat tgttcatctg ccttataaat aatgcttata tgtgcatgct gatgataaat
     5341 gactgcctga ttttctccac ctcttttctg ataagaagat aaaaagacat catccagctg
     5401 ctggctgcgt aaatccatcc cttcatgcaa ggcttcttta ttcggtacat ccatcagttt
     5461 cccggtcggc agcaggcgct catccaactc ccattgctga tctgctgtca gggagaataa
     5521 cgagctttct tctggaaaaa caaatgtagt atgatagccg attccccatg gaaacgcttc
     5581 ctttccattg ttcatgaccg tcgcatgttt aaatagagta ttccccttta gcgtgtatgt
     5641 catccgaacg acggcatgat gcggaaattg cttatacaca tggggaagct ctgaaaggtc
     5701 aatttcagtt tcaacgatca cttcttcatc agtttgcttc atggtcacta cgttccattt
     5761 ttcctgataa agaaacccat gaagatggtt gtgtttatcc ttttcattga tatcaaaatg
     5821 atatgttctg ccccggaaac tgaatgtacc gtcacttatc cggttaggcg gaaacaggat
     5881 cggaatgcca tacaatgttg gtgtatcatg aaagctttct gctgtttccg gttcacgcaa
     5941 aagctgaagc tgtgtcgttt tgtccacaag agaaataacg ttgctccccc actcgggaac
     6001 gacaatcatt tctatatgtt cgttgcctgc tttgatggca ggtgttccta aatacgtaat
     6061 cttctcaata tcgtttgcca tgtgtactca acctctcctt gattcattgc ttgtataaga
     6121 attcaatgta atgtgccata tttcctccat ttatgttttc tctttatcaa acaaaaaaca
     6181 ccgttcaaat gttgaacggt gtgattcggg atgctattgg atgagacgtg taatctgttt
     6241 ctttttccaa acgaattgat aatacccata aatcaacagc aagctgacgg caaacgcagc
     6301 cggatagcca acccagacgc cgagtatttc aagatttgtg tagtgggaaa gtacgaacgc
     6361 gacaggcacc tctactcccc agatggcaaa aatactgata acggtcggcc ataaaaccgt
     6421 tccgcttgcc cgcatcgtcg cagaaataat ttgggcattt ccaaagagta agtaactcca
     6481 caatgtaatc atcaagagac ggtgcgcaat gtaaagcgtc tcttgttctg ttaaaaataa
     6541 tcctaaaatc tgatgtgaaa aaacatagat cagaatgatc agcacaccgc caatgatata
     6601 attcagccaa atcccgacac gaattacctg ttttaggcgg ccaaactcgt tcgctccgat
     6661 cgattgcgcg gcgaatatag acaccgcaat accaaggctg acggccggca tctgcacata
     6721 gctggctacc tggttgacca cgccgtatgc agctgtcgca ctagagccat aatggttgac
     6781 gaatgaaata accgctatct ctgataatga gacgagaatc atattgatgc ttgatggaat
     6841 cccgaggcgc agcaggagag ccagcagttc tttatccatt ttcaaataac ggcgcactgt
     6901 tttatcaaat tgcagcggat ggtttcgttt tcgcaaatac accattaaca caataaaagt
     6961 cgcaatcgtg gaaataacgg ttgcatatgc ggagccgtat atgcccagtt tcggaaaacc
     7021 gaacatgcca aggatcaaga caggcaaaag cgctatgtta atgaccgtgc tcacaatcag
     7081 cgtataaaaa ggtgtcttag aatcccctgt tccgcgcaaa aatgttgtat aagcgaaata
     7141 taaaaacata aacggcatcg cgtaaaataa aatacgtgca tagctggcgc tgacatgaat
     7201 tacattttca ggcgtgccca ttaaacgaag aatatccaag gtgaaaatac tgccgatgac
     7261 ggctaacaca actcctaata aaaacgtaaa cgtgagcgtc gtcccgacaa cagcctttaa
     7321 ccgttcctca tttttcgccc cgtaggcttg tccgatcagg attgagcttc ctgaaccgat
     7381 cccaattgta aatgagatta aaagaaaaaa taacgggaaa aaggatgata cagctgcaac
     7441 cgcatcaaca ccaagccatc tgcctacagc catcatcccg actagctggc cgactgattg
     7501 cagtacattg cttagcagca gcggaactaa aaagagtgac atagaccgcc agacagatgg
     7561 attgcttttc ttctgagtta aaagttgttc ttgtgatgtt tccatattga aagctccttt
     7621 atttgcattc gtttgtaaac atgatcctat tcttttgaca aaaacaggat atgtaacatg
     7681 atattattca tcactgtaca tatgaaaccc ttcccgattc ttctaatttt acacatcttt
     7741 ttagcttatg aagaataaac tagcaagtaa cagcctattt tcttgtgagt aaaagaataa
     7801 aaaataattt atgcaaaagt attgtaatct atccgtaatt attgtaacat ttgtaacata
     7861 agagaaagag attttgaagg agtgaactac aatgaaaaaa tgttttctat ttctaacaac
     7921 tattgcactt attctgtcat taagcacaaa tgcatttgcg aaaaatactt caggtgattt
     7981 atcacaaaaa caagcactgc agctggcgtt atcagcaaga gagcattttt ggaacacaat
     8041 gagcggccac aatccaaaag cgaaaaaagc agtttgccca tcaggcactt ttgagtatca
     8101 aagtcttcaa tacgtgtaca tgtgcagtga cctaggcact aaagcaaaag cagtgaatta
     8161 cttaactcct attttcacaa aaacagcaat cgaaaaaggc tttaaagact atcacttcac
     8221 tgtttcaaaa ggaaaactag ccgtgccaat cggcgacggt gacaaccttt taaattggaa
     8281 aaaatcaact gctaaactga tctctaaaaa aggcggcaca gtcacatacg aatttaccgt
     8341 tcctacttta gacggatcac ctgcagcgaa acggaaagtg acatttgtaa aagaaaataa
     8401 aaaatggaaa gtcaatcaat ttgacgcagt tatataagaa aaaacgccct gcataaggga
     8461 cgggactgac ccccgttttt g
//
