LOCUS       NZ_JALATE010000009     10366 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain WR2FB-3 Contig_0000009, whole genome
            shotgun sequence.
ACCESSION   NZ_JALATE010000009
VERSION     NZ_JALATE010000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 227458)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 227458)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 448.1512087568532x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:29:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,311
            CDSs (total)                      :: 4,228
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 89 of 146
            Pseudo Genes (incomplete)         :: 78 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 38 of 146
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:58:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 91617
            Orig. end                         :: 101983
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALATE010000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10366
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10366
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10366
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5366](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5366)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(179..649)
                     /locus_tag="MOD98_RS17665"
                     /old_locus_tag="MOD98_17625"
     CDS             complement(179..649)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014479885.1"
                     /locus_tag="MOD98_RS17665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17625"
                     /product="hypothetical protein"
                     /protein_id="WP_268280438.1"
                     /transl_table=11
                     /translation="MWKALILLKKQKNQSSIDEGYLQIPEKDFNLELKQMIFELESLGE
                     AVSEKLQNKYIDVFEAQGKPNLSIKVCRDIDGDVINDLTAERCFEKEYRSQITINYYQP
                     AKAYDEDIDCITMDLWYYYGGFIGSGCGTLFDLKNNDLEKEIEEVLIFLLND"
     gene            1198..1710
                     /locus_tag="MOD98_RS17670"
                     /old_locus_tag="MOD98_17630"
     CDS             1198..1710
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MOD98_RS17670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MOD98_17630"
                     /product="hypothetical protein"
                     /protein_id="WP_268280437.1"
                     /transl_table=11
                     /translation="MKKTVLSTISLGLLFSTVAPLTSHAESISEPTPSYITQVNSTPLQ
                     ELIKTSSVPVIQENGEISIQAVPPSTKWKVVDHFRGNSKNIDTAAELGARIAAGLPGLK
                     YSWAGIFAQAVFGTFYHKKPTRYYSTTQYLAYDKKYRYTIVDMYVYKDSKYSKQIGYDY
                     KVFRTPR"
     gene            complement(1894..2106)
                     /locus_tag="MOD98_RS17675"
                     /old_locus_tag="MOD98_17635"
     CDS             complement(1894..2106)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328394.1"
                     /locus_tag="MOD98_RS17675"
                     /note="Built to rescue LMOF2365_14255 during structural
                     annotation change regression.; Built to rescue
                     LMOF2365_14255 during structural annotation change
                     regression; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17635"
                     /product="bacteriocin-like WGxF protein"
                     /protein_id="WP_268280436.1"
                     /transl_table=11
                     /translation="MKHITFAISTSILIIITGIVHRVIFRLFGFPFDEALFFWGGFVVV
                     YFVIALIASLLFRNVGSSSSRRYQA"
     gene            complement(2120..2740)
                     /locus_tag="MOD98_RS17680"
                     /old_locus_tag="MOD98_17640"
     CDS             complement(2120..2740)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328395.1"
                     /locus_tag="MOD98_RS17680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17640"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_268280435.1"
                     /transl_table=11
                     /translation="MIELKSISKSFDGKSVLSNFSIHIEENEFVSIVGKSGSGKTTLLN
                     IISLLDSPDEGEIEILGYKNPKSKEVMKLRRENLGYIFQNYVLMDNETVLTNLLLSTAY
                     AKDFDKNKLSEALEMVGLEKSFLKKKVYQLSGGEQQRVAIARIILKPCDIILADEPTGN
                     LDEYNKNIILSLFHQLKEMGKTIICVTHDPEIANCSDRVINLS"
     gene            complement(2737..4908)
                     /locus_tag="MOD98_RS17685"
                     /old_locus_tag="MOD98_17645"
     CDS             complement(2737..4908)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="MOD98_RS17685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17645"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_268282713.1"
                     /transl_table=11
                     /translation="MKKTIYILFILFLLSMNISSYHYTLNKQVFYTLFSNKQTLIINYN
                     KAHFKSDDFIKEIAGFSKKENVNITQYNFLDEKTLNIYASNLKNEPSIKIGDRFISNFN
                     KTANESNKNLIGTIDFPLSTWKIRYFNLNQISNVGLGNRFYISSEDEDVIEQAKKLFSD
                     YGDVSIEENISLNDSAVFSTALLLLALLSMLILFIGILYFVIKNRKQLLLQKLWGYSKT
                     KSLLTFPKMFLKPLVMIIICGALLTVILAFIFNLNDWLSVYVKMYMRNALIVTFSMMVY
                     TIIVTLILYNNSNISATIKGGIPFKKFQWLSLGFKLIITILLFNIFASSLSNLFDLNQR
                     LDNQSYWNKTQTVYKTSFADTGLNYSDLKIDREKNEKVRKLYHELERHKDAFIMDAQNY
                     GILKMNGKTLVYFYTLNTTKENGIYSPSGRSVTISPNYLKVNTIKGINGKTITEKQLTF
                     DQNTLNLLVPKKYAKYKEKILKAYKNQFFFDKVEVDNMYNKEIQQPLNTLKKERLKIKI
                     IYTQNNQEYFTYNSMLGNNKNNIVDPIAIVFTDNVDSSVIGAYATSSLFFSDASKGMAY
                     DHIIPYLDKTDTKELIGSAISVYQELSDQIARIQNQFIQNLIGLTITLILSVAFLISYI
                     WSYYSANAYRLYLKELFGYSYWARNKSLIILSTVSNCLIGICFSIYYKIYELIGLIVLF
                     FGIEMIILYFLSLYLNRKNMNKILKGDRL"
     misc_feature    complement(4892..4903)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.95"
                     /tool="antismash"
     gene            complement(5001..5366)
                     /locus_tag="MOD98_RS17690"
                     /old_locus_tag="MOD98_17650"
     CDS             complement(5001..5366)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="MOD98_RS17690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17650"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_268280433.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.1e-07,
                     bitscore: 25.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKVFSSMVLGATLLVSSSVIFASSSDEATHGEVNLDETRDLIQ
                     IMKLTRTKAGGGTWYHGFEDGQVKSNYNHTKKTHKSSAKAGGERFFSSKWKKKDEGYTY
                     ASVYQTLWGNRAFWDTK"
     misc_feature    5487..5502
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            complement(5794..6126)
                     /gene="csaA"
                     /locus_tag="MOD98_RS17695"
                     /old_locus_tag="MOD98_17655"
     CDS             complement(5794..6126)
                     /codon_start=1
                     /gene="csaA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389785.1"
                     /locus_tag="MOD98_RS17695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17655"
                     /product="chaperone CsaA"
                     /protein_id="WP_268277082.1"
                     /transl_table=11
                     /translation="MAVIEDFEKLDIRTGTIVKAEEFPEARVPAIKLLIDFGAEIGMKQ
                     SSAQITKRYQPEDLINKQIIAVINFPPRRIAGFKSEVLVLGGIPGQGDVVLLQPGQSVP
                     NGTKIG"
     gene            complement(6191..6916)
                     /locus_tag="MOD98_RS17700"
                     /old_locus_tag="MOD98_17660"
     CDS             complement(6191..6916)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389786.1"
                     /locus_tag="MOD98_RS17700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17660"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_268280487.1"
                     /transl_table=11
                     /translation="MLMDSLQEIICEKRTYTRLYHSHKHAYSQFLFPLEGSIDLETEGR
                     EVKLKPDRFLYIPPECEHRFRSIGRNECLVLDVPLYAMKIDEYHAGSGIEAALDPFWSS
                     IRYLLTEEAKAGTANSLHMLVQYIKEKLQSHTCDSIAYIHSHLFERLTIKKLAEIEHYH
                     PAYYSSWFKKQTGKSPQSYIANLRLEEAKRMLIEKKETLTNVSEALGFQNLSSFTRWFT
                     KSTGMPPRLYRNTLYSDKK"
     gene            complement(6931..7674)
                     /locus_tag="MOD98_RS17705"
                     /old_locus_tag="MOD98_17665"
     CDS             complement(6931..7674)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389787.1"
                     /locus_tag="MOD98_RS17705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17665"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_268280432.1"
                     /transl_table=11
                     /translation="MDDIHKIEQLAAASWPSYIQKSMGQWLLRANFGVTKRANSVWTSA
                     DMPEGEFQQEAEHFYKSLGLPVCFHISNASPKGLDGVLADRGYEKADECFQMTALCRDI
                     MNRTEDNRRFTYKWEQVPSSVWIDNFIQLEEFSPERHRGYAYIFERMPPCKTFFTMYDQ
                     GSLTAVGTASVIDGYGGLSNIVVAKEHRGKGAGTQVIRVLTEWAISNGAKHIYLQVLKE
                     NLAAVSLYEKIGFSPISEHHYRIQK"
     gene            complement(7752..8327)
                     /locus_tag="MOD98_RS17710"
                     /old_locus_tag="MOD98_17670"
     CDS             complement(7752..8327)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389788.1"
                     /locus_tag="MOD98_RS17710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17670"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_268280431.1"
                     /transl_table=11
                     /translation="MSPRIGLNQKMIVDAAAEIADQEGLNSVTLAALSKKMNVRPPSLY
                     NHVNGLQAIRTELAVNGLTKLFDQMAASVTEQKGDAALLYLAHAYVDFAVGNPGYYEAA
                     LLKVQDKRAEIVSDQIVQLVTNLLIENRYACEKTAIHATRGLRSLLHGFTALIAKEAFE
                     REEDISESLSFSIRTFLSGLSINNKNIM"
     gene            complement(8332..9033)
                     /locus_tag="MOD98_RS17715"
                     /old_locus_tag="MOD98_17675"
     CDS             complement(8332..9033)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389789.1"
                     /locus_tag="MOD98_RS17715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17675"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_268280430.1"
                     /transl_table=11
                     /translation="MRVTQYQTVWQLTFFPVLFPVNCYLVEEENEVTLVDAAIPSSYKG
                     IIQAVNQLGKPLQHILLTHAHGDHVGSLDTLTQAFPHAKVMISERDSFLLQGDTSLRHD
                     EPQTPIKGGIPKHVQTKPHHLLTGGETIGSLLAIPTPGHTPGSMSFLDTRNGIIIAGDA
                     FQLRGGIAVSGQVKWTFPFPAFATWNKEEAIKSAQLLADKAPSCLAVGHGNFLRSPSER
                     MRQAIQKAKKG"
     gene            complement(9110..9592)
                     /locus_tag="MOD98_RS17720"
                     /old_locus_tag="MOD98_17680"
     CDS             complement(9110..9592)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258635.1"
                     /locus_tag="MOD98_RS17720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD98_17680"
                     /product="GyrI-like domain-containing protein"
                     /protein_id="WP_060398811.1"
                     /transl_table=11
                     /translation="MSFSHITHLKQKRFAGLSQRTSNALEMTEERRIPALWDQFWQQDM
                     SILFSQAEKDQSIIALYSNYEQETNGFYTFSVGAFQEDSGTLPEPYENIDLPASAYAVF
                     TSRIGPIEEIVYETWKEIWTWDKRHLRTFTGDFEMYDQSAAVPQRAQVNIYVAIKN"
ORIGIN
        1 cttcagagtc aaccggagaa acagaaacat ttaaaaattg tacagagtta agaaagaaat
       61 atccaaatgg agtgcctagc tcccaccctg cttatcaatc taaaatggac agggatcatg
      121 acaactatgc atgtgaacgt taattgaaag ccctttgtgg ctttcttttt tatatttgtt
      181 aatcattgag taaaaatatt agaacctctt caatttcttt ctctaagtca ttatttttta
      241 aatcaaataa tgttccacaa ccgcttccta taaagccacc gtagtaatac cacaaatcca
      301 ttgttatgca gtctatatct tcatcatagg cttttgcagg ttgataataa ttaattgtta
      361 tttgtgaacg gtattctttt tcaaagcatc tctcagctgt taaatcattt attacatctc
      421 catctatgtc tctacaaact ttgatgctaa gatttggttt accctgagct tcaaaaacat
      481 ctatatactt attttgcagc ttttcggata ctgcttctcc caggctctcc agctcaaata
      541 tcatttgttt taattctaga ttaaaatcct tttccggaat ctgtagatag ccttcatcga
      601 ttgaactttg atttttctgt ttcttgagta atattaaagc tttccacact atgttgtcct
      661 cctattaatt aaagcaaaaa aagggaaccc tatgacctaa agttacacta gctagtacat
      721 atacaataat cggggtgtat aatttgtggc tgatactccg catctcctgc tgttagaaca
      781 caagtttccc gcaataataa aagatggctg ctgtttacct tcctttttat tacgaaagta
      841 tgaacggcag ccttattgat ttagaatacc caaaaaataa tcacaagcca ggagataatc
      901 aaagttaaaa tgaaccattt ttcctgcaaa gaaattaaag cgtagcaaat ataagagata
      961 gttaacatca caatagaagt gatcttaaca taccatccaa tagattcatt ggagaatgca
     1021 taaacaaata aagagataag aacaagaacc ataaatatgt tccctacagt ctttaatata
     1081 ctctttttgt catcaaagtt cataatttca acctccaaaa tatgtatatt atatgttata
     1141 attatacagc atccagatag tcataagcat taaaaaactt gaaaggatga ttgtgaagtg
     1201 aaaaaaacag ttctatctac catatcactc ggactcctat tctcaacagt ggctccacta
     1261 actagccatg cagaatccat ctcagaacct acaccaagtt atatcactca ggtcaattca
     1321 acaccacttc aagaattaat taaaacatca tcagttccag tcattcaaga gaatggagaa
     1381 atttcaattc aagctgtgcc accatcaaca aaatggaagg ttgtagatca ttttagaggg
     1441 aactctaaga acatagacac tgcagctgaa ttaggagcaa gaattgctgc agggttacct
     1501 ggtcttaaat actcctgggc tggtatattc gctcaagctg tgtttggaac attctatcat
     1561 aaaaaaccaa cacgttatta ctcaacaacc caatacttag cctacgacaa aaagtataga
     1621 tacacaatcg ttgatatgta cgtctacaaa gacagtaaat atagcaaaca aataggttat
     1681 gattataaag ttttcagaac tcctagataa tatgtcagat ataaagccct tttaaagggc
     1741 ttttaacttt ttaaacaaaa agccagagag tatgtgaggg aactctctgg cctatagtgg
     1801 ttgatacacc aactttgata catatacagg atactttata tttgggatgt attcaagata
     1861 aggtatattt tattgtttaa tgaccagatt gagttacgcc tgatatctcc tgctgctaga
     1921 agaaccaaca tttctaaaga gtaaggaagc aatcaaagct ataacaaaat aaacaacaac
     1981 aaatccgccc cagaagaaaa gcgcttcatc gaatggaaat ccaaatagtc taaaaataac
     2041 cctgtgaaca ataccggtaa taataatcag gatgcttgta gaaattgcaa aagtgatatg
     2101 tttcatcaag aacaactcct tacgaaagat ttattactct atcagagcag ttagctattt
     2161 ctggatcatg tgttacacat atgattgttt tccccatttc tttcaactga tggaaaagag
     2221 aaagaataat gttcttgttg tactcatcaa ggtttccggt tggttcatcg gccagaatga
     2281 tatcgcaggg ttttagaata atcctggcta ttgctacacg ctgttgctca ccaccgctca
     2341 actggtatac ctttttcttt aaaaagcttt tttctaagcc gaccatctct agagcttctg
     2401 acagcttgtt cttatcaaag tcttttgcgt atgctgtgga aagcaataaa tttgttaata
     2461 cagtttcatt atccatgagc acatagtttt gaaagatata tcctaaattc tctctcctta
     2521 acttcatcac ctctttagat ttaggatttt tatatcctaa aatctcaatt tcgccttcat
     2581 ctggagaatc taaaaggctg attatattta gaagagtggt tttgccagaa ccgctctttc
     2641 caacaataga aacgaattca ttttcctcta tgtgaattga aaagttagaa agaactgatt
     2701 ttccatcaaa acttttggat atgcttttaa gttctatcat aatctgtctc cttttaaaat
     2761 cttattcatg tttttcctgt ttaaataaag actgaggaaa tacagaataa tcatttcaat
     2821 tccaaaaaac aatacaatca agcctattaa ttcatagatt ttataataaa tactaaagca
     2881 gattcctatc aggcagttag acacagtcga taatatgatt aagctcttat ttctagccca
     2941 atatgaatat ccaaataact ctttaaggta aagtcgataa gcatttgcgc tgtagtatga
     3001 ccatatataa gagataagga aggctactga caatatgagt gttatagtta atccaattag
     3061 attctggatg aactgatttt gaattcgtgc tatttggtcg cttaattctt gatagaccga
     3121 tatcgcagaa ccaatcagct cttttgtatc tgttttatct aaataaggga tgatgtggtc
     3181 atatgccata ccttttgagg catcagaaaa gaataaactt gaggttgcat atgcacctat
     3241 aactgatgaa tcaacattat cagtgaatac aattgcaatt ggatcaacaa tattgttttt
     3301 attgttgccg agcatactat tataagtgaa gtattcttga ttattttgag tatatattat
     3361 tttgattttc aaccgctctt ttttcagcgt attcaatggt tgctgaattt ctttgttata
     3421 catattgtct acttcaacct tatcaaagaa aaattgattt ttgtaagctt tgaggatttt
     3481 ttctttatac ttagcgtatt tttttggaac aagtaaattc aatgtgtttt gatcgaaggt
     3541 taattgtttt tcagtaatcg tttttccgtt aattccttta atggtgttta cttttaaata
     3601 attaggacta atcgttacgc ttcttccgga tggtgaataa attccatttt ctttggttgt
     3661 attcaatgta tagaaataaa ctagagtttt cccattcatt ttcagtattc catagttttg
     3721 agcatccatt ataaacgcat ctttatgtct ttctaattca tgataaagct ttcgtacctt
     3781 ctcgtttttt tctctatcaa tttttaagtc actgtagttt aaacctgtat cagcaaaact
     3841 ggttttataa actgtttgtg tcttattcca ataagattga ttgtctagcc gttggtttaa
     3901 atcgaacaaa ttagagaggg atgaagcaaa aatattaaaa agtaggattg ttataattaa
     3961 cttaaaaccc aatgacagcc attgaaactt tttaaatggt ataccgcctt ttatcgtggc
     4021 gcttatattg ctgttgttat ataaaatcaa agtaacaata attgtgtata ccatcattga
     4081 gaatgtcaca atcagtgcat ttcgcatgta cattttaacg tacactgata accagtcgtt
     4141 taggttgaat ataaaagcca gaattacagt gagaagggcg ccgcaaatta tgatcataac
     4201 aagtggtttt aaaaacattt ttgggaaagt taacagtgat ttggttttcg aatatcccca
     4261 tagcttctgt aagagaagtt gcttgcgatt tttaattacg aaataaagga tgccaataaa
     4321 taagattagc atggaaagca aagctaacaa gagcaatgcg gtgctgaaca cagcagagtc
     4381 atttaaactg atgttttctt caatactgac atcaccatag tctgagaata gctttttagc
     4441 ttgttcaatt acatcttcgt cctcggatga aatataaaat ctatttccta accctacgtt
     4501 gcttatttgg tttaaattga agtatctgat tttccatgtt gataaaggaa aatcaatagt
     4561 accaatcaaa tttttatttg attcatttgc tgttttattg aaattagata taaacctatc
     4621 tcctattttg attgaaggtt cattcttaag gtttgaagcg tagatattta aggtcttctc
     4681 gtctaaaaaa ttgtattgag taatattcac gttctctttt tttgaaaatc cagctatttc
     4741 cttaatgaaa tcatcacttt taaagtgtgc tttgttgtaa ttaataatta acgtttgttt
     4801 attagagaat aaagtataaa aaacttgctt attcagagta tagtgatagc tggatatgtt
     4861 cattgagagt aaaaatagaa tgaacaatat ataaatcgtt ttcttcattg gatagcctcc
     4921 ataaaaagaa atccggtgcc ttatattcaa gttttgacgg caccggaagt agattaaaaa
     4981 tcactttgtt attgtctgtt ttatttcgta tcccaaaaag ccctatttcc ccaaagagtt
     5041 tgatacactg aagcgtaagt atacccttca tcttttttct tccatttaga tgaaaagaac
     5101 ctttctccac cagccttcgc acttgatttg tgagtctttt tagtatgatt gtaattggat
     5161 ttaacttgcc catcttcgaa accgtgatac caagtaccgc cacccgcttt ggttctggtc
     5221 aatttcatga tttgtattaa atcccttgtt tcatccaagt tcacttctcc gtgtgtagcc
     5281 tcatctgaag aactagcaaa aatgacagaa gatgatacta gtaaagtagc tcccaacacc
     5341 attgaagaaa aaactttctt tttcaatata acctctcctt tttgttttca caagaaacca
     5401 ggtcacagtt accccttgta ttcaccatta agtacgtttt cgtttgttta aaggcatttc
     5461 tgcctattct taaaatggtg tttgaggtca cagttaagtc acattccgtg ttattgttga
     5521 caaactcatt attgtcgaac aatcatcatc atacagtaat atttgaatta ctcacgatta
     5581 atttagacaa aataagacaa gcttttataa ctatttcact cacaattaaa aagttaccct
     5641 tgcattcctt cagccgccat gctatattca tatagcgatg agctgaattg tgtaagccga
     5701 tgtacatgcc ctttgcggca cacacgaatg tggcgtgtgg tgcatcgcct caatacgcaa
     5761 agacgtttgc ctttggcaaa cgtctttttt atgttatccg atttttgtgc cgtttgggac
     5821 ggattgtccc ggctgcagca ggacgacatc gccctggccg ggaatgcccc cgaggaccaa
     5881 aacttccgat ttaaatccgg cgatccggcg cggtggaaag ttgatgactg ctatgatttg
     5941 cttgttgatg agatcttccg gctgataacg cttcgtgatt tgcgcgctcg attgtttcat
     6001 gccgatttct gctccaaaat caatcaaaag cttaatggcc gggactcttg cttcaggaaa
     6061 ttcctccgct tttacaatag tgcctgtcct gatatccaat ttctcaaagt cttcaatgac
     6121 tgccatatca ataactcctt tttctcctat tataaaagag gtgtatgagg tttgtttaac
     6181 caaaggacca tcatttttta tccgaatata aggtatttcg atatagccgg ggcggcatgc
     6241 ctgtactttt cgtgaaccat cttgtaaatg aagatagatt ttgaaagccc agcgcctcgc
     6301 tcacgttagt aagggtttcc tttttctcta tcagcattcg tttcgcttcc tcgagacgaa
     6361 gattggcaat atagctctgc ggagacttcc cggtctgctt cttgaaccag cttgaatagt
     6421 aggctgggtg gtaatgctcg atttctgcca gcttcttaat cgtcagccgc tcaaataaat
     6481 ggctgtgaat gtaggcaatt gagtcgcagg tatgggattg cagcttttct tttatgtatt
     6541 gaacaagcat atgcagcgag tttgctgtgc cggcctttgc ttcctccgtc agcaaatacc
     6601 tgattgatga ccagaaagga tcaagtgcag cttcaattcc cgatcctgca tgatactcat
     6661 caattttcat cgcataaagc ggaacatcca gtacaagaca ttcatttctg ccgattgaac
     6721 ggaaacgatg ctcgcactca ggcggaatat ataaaaaccg atccggcttt aatttcacct
     6781 cgcgcccctc cgtctcaaga tcaatactcc cttcaagcgg aaaaagaaat tggctgtacg
     6841 catgcttatg tgaatgataa aggcgtgtat atgtccgttt ttcgcaaata atctcttgaa
     6901 gcgagtccat cagcaagccc cttcctttgg tcatttttgg atccggtaat ggtgttccga
     6961 tataggcgaa aatccgattt tttcatataa agaaactgcc gcaaggtttt ctttcagcac
     7021 ttgcagatag atgtgcttcg ctccgttgct gattgcccat tcagtcaaca ccctgattac
     7081 ttgagttccc gccccttttc ctctatgctc cttggctaca acgatgttac tgagtccgcc
     7141 atagccatca atgacagaag ccgttccaac cgctgtcagg ctcccttggt catacatcgt
     7201 gaaaaaggtt ttacacggcg gcattcgttc gaaaatgtat gcataacctc gatgccgctc
     7261 aggtgaaaat tcttccaatt gaataaaatt atcaatccaa acagaagacg gtacctgttc
     7321 ccacttatat gtaaatcggc gattgtcttc tgtacggttc ataatgtccc ggcacaatgc
     7381 agtcatttga aaacactcgt ctgccttttc atagcccctg tctgccaata cgccatcaag
     7441 ccctttcgga gatgcattac tgatatgaaa acaaacaggc aagcctaagc ttttataaaa
     7501 atgctcggcc tcttgctgga attctccttc aggcatatcc gcagaagtcc atacgctgtt
     7561 cgcccttttg gttacgccaa agttggctct gagaagccac tgtcccatgc ttttctgaat
     7621 ataagacggc catgaagctg ccgctaactg ctcaatttta tggatatcat ccatagcata
     7681 atgtctctcc cctatgtata ctaccatgac attatacaat attcagaaat ataagacatc
     7741 tatttattgg tttacataat atttttattg ttaatagata atcctgacaa aaacgtccga
     7801 attgaaaatg acagactttc tgaaatatcc tcttcacgtt caaatgcttc ttttgcgatt
     7861 aaagcagtaa agccatgaag caaacttctg agcccccttg tcgcgtgaat ggcagtcttt
     7921 tcgcatgcat atctattttc aatgagcaga ttcgtcacaa gctggacaat ttggtctgac
     7981 acgatttccg cccttttgtc ctgaacctta agcaaagccg cctcataata tccgggattt
     8041 ccaactgcaa aatcaacata tgcatgagct aaataaagca gagcggcatc tcccttttgt
     8101 tcggttacag aggctgccat ttgatcaaaa agctttgtca gcccgttgac agcaagctct
     8161 gttctgattg cttgcagccc gttgacatga ttatacagcg atggagggcg aacattcatt
     8221 tttttagaca gtgcggcaag cgtgacacta ttcaagcctt cctgatcggc aatctccgct
     8281 gccgcgtcta caatcatttt ttggttcaat cctattctgg gtgacatgct atcatccttt
     8341 ctttgctttt tgtatcgcct gtcgcatccg ctctgagggt gatctcagaa aattcccatg
     8401 tccgacagcg agacaggaag gcgccttgtc ggcgagcaat tgtgcgcttt tgatggcttc
     8461 ctccttattc catgtggcga aggcaggaaa aggaaacgtc cattttacct ggcctgatac
     8521 agcaattcct ccgcgcaact gaaatgcatc tcctgctatt ataatgccgt ttcttgtatc
     8581 aagaaatgac atgctgcccg gtgtatggcc cggtgttgga attgcaagca gagaaccaat
     8641 tgtttcaccg cccgtcagca gatggtgagg ctttgtttgc acgtgtttcg gaatacctcc
     8701 ttttataggt gtctgcggct catcatgacg caaggatgta tcaccttgca gcagaaaaga
     8761 atcccgttct gagatcatca cctttgcatg aggaaacgct tgtgtcaatg tatccagtga
     8821 tccgacatga tccccatgtg catgggtcaa taaaatatgc tgaagcggtt ttcccagctg
     8881 atttacagct tgaataattc ctttatagct gcttggaatt gcagcatcaa caagggtgac
     8941 ttcgttttct tcctctacta aatagcaatt gaccggaaac aaaactggaa aaaaagtcag
     9001 ctgccaaacc gtttgatact gggtaactct catcttttta ttccccctaa caaaaaacta
     9061 atggtattag tttcattata actaacacca ttagcttttc aagagtttat caatttttta
     9121 tggcgacata tatattaact tgcgcccgtt gaggtactgc tgcactttga tcatacatct
     9181 caaagtcgcc tgtaaatgtg cgcagatgac gcttatccca agtccaaatt tccttccacg
     9241 tttcatacac aatctcttca atagggccga ttctggaagt gaaaacggca taagcggaag
     9301 ccggcaaatc aatgttttca tacggttccg gaagagtccc gctgtcctcc tgaaacgctc
     9361 cgactgaaaa tgtgtaaaac ccattcgtct cctgttcgta attggaatat aaggcgataa
     9421 tgctttgatc tttttcagcc tgtgagaaaa gaatgctcat atcctgctgc caaaattggt
     9481 cccatagggc cgggatccgc ctttcttctg tcatttccaa tgcgttgctt gtccgctgtg
     9541 atagtccggc aaaccgtttc tgttttaagt gtgttatatg tgaaaaactc atatgttccc
     9601 tcctttttac ctagattata aaaaaagaaa cctgacagca gtgtgtcagg tttcatatac
     9661 tttttgcatc tgatgaatgg tatcttttac tttatcccgt atatgaaggg gttccaaaac
     9721 ctcaatatcc ttcccgaact gcagcaaaaa accgatcagc cattgatctt ctggaagaga
     9781 aatcactgcc cggcagttct catcttcatc acaatgaaga acctcagatc cgaaccattc
     9841 acgtatgcgc tgacgtgctg gctgctgaac aagaataacc agttcagtta aacggtcctt
     9901 ttcgtaccaa ttcctatccc acggcaaggt atccagctga atgtcttttc tgataaagga
     9961 attttgtaat atggccatat cttttatccg gttaagttta aaaaagcgaa aatcgtttct
    10021 aaccagacaa taggcgtata aataccagtg ccccgccttg caaaccaatg tgtagggttc
    10081 tgtttcacgt agaagtgttt ccccgctggc gttcgtatat gtaaatgata tcgttgacaa
    10141 ttgatcgatg gcggcactga ttttctcacg aagtgccttt tgatcctcag tatggcccca
    10201 agccgtcatg tcaataaacc atttttctgt ttgatgctta aatgcttcag cagattgctc
    10261 cggtatgagg tgcttaattt tttgataagc tgtgctgtga gaagcaggtt cacacatgga
    10321 agagacactc tgcaaagcgg aagcaatcgc aaacagctcc tcctct
//
