LOCUS       NZ_JALAQD010000032      7782 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus atrophaeus strain CK3O1B-51B contig_0000032, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAQD010000032
VERSION     NZ_JALAQD010000032.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.59696795022654x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 01:57:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,269
            CDSs (total)                      :: 4,192
            Genes (coding)                    :: 4,034
            CDSs (with protein)               :: 4,034
            Genes (RNA)                       :: 77
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 1 of 158
            Pseudo Genes (frameshifted)       :: 83 of 158
            Pseudo Genes (incomplete)         :: 107 of 158
            Pseudo Genes (internal stop)      :: 32 of 158
            Pseudo Genes (multiple problems)  :: 55 of 158
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:50:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7782
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAQD010000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..2354
                     /locus_tag="MOD08_RS21115"
                     /old_locus_tag="MOD08_21065"
     source          1..7782
                     /db_xref="taxon:1452"
                     /mol_type="genomic DNA"
                     /organism="Bacillus atrophaeus"
                     /strain="CK3O1B-51B"
                     /submitter_seqid="contig_0000032"
     region          1..7782
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..7782
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7782
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<4:2354](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <5..2354
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             <5..2354
                     /NRPS_PKS="Domain: PCP (191-258). E-value: 3.4e-18. Score:
                     57.6. Matches aSDomain: nrpspksdomains_MOD08_RS21115_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (402-587). E-value:
                     8e-14. Score: 43.3. Matches aSDomain:
                     nrpspksdomains_MOD08_RS21115_Condensation_LCL.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="MOD08_RS21115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD08_21065"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_268519685.1"
                     /sec_met_domain="AMP-binding (E-value: 1.3e-39, bitscore:
                     124.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.6e-15, bitscore:
                     44.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.5e-16, bitscore:
                     47.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NSQVYILDKNKNLLPPGVIGCIYIGGVGLAKGYLHNPKLTTEKFI
                     DNPYIKGEKMYCTGDLAKWSADGNIEFVGRKDHQIKMRGFRIEPQEIEYQIMQHRDIKE
                     AAVIDIVLEDNSKQLWAFIVSREENDSQLLRDFLQDKLPDYMIPIRFYSLKRLPLTSNG
                     KIDRNSLKEKYKKAIHERHKIEKPTKEMEKILYEIWKDVLKTEGMDINDDFIQVGGHSI
                     NAVQIMSRLQKKGIKLTIDDLYKYKTIKRLSNMLTASSVSNKIYNIYDAEKLLRKECDL
                     NVYIKRINVKNKTRYIYFVDNHIAKENEVLAAIKKYISPEIQPHYIKFNKNNVDFGHEK
                     WNEILELKSLTKDEVNFITDSCVNNNQLSAHFRKGEVIKTFPLSPIQRYHLDYSDYSGT
                     IVFFNEICEISALKKAITIFVEKQELMRSMLRNRDGEYYWDVYSCEEHINIPFLDISEY
                     NLDSQKELLKDILPKMFFSEYSNSLFYRILIIKVNEKEHILLFPFSHLKFDKMSSILIE
                     KEIKSIYNSIEKNESLPTHNINLYSDYVKQVMKGPQLISDQEVNNIYDLNKMVSLHELI
                     SVPSNETRPVIYTLRKKIDHTQNHLETYLQICCSVLSKMLGIKDVPIFIIQHGRNTQDG
                     NYFGTLGEFIDIFPFIWFETDTIESVNNRLNTRIAIKKNKNINFAALFYQQDIKSNFPK
                     TNRNITKMLSEYPILFNYLDETKESDNEYNQLMKSNTPLNTGKRIIVEVNKNEEHLNFT
                     FILPFRLKEITCDGVIESLVAQQGDTNEKL"
     CDS_motif       48..137
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOD08_RS21115_0001"
                     /evalue="4.10E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="MOD08_RS21115"
                     /protein_end="44"
                     /protein_start="14"
                     /score="41.5"
                     /tool="antismash"
                     /translation="PPGVIGCIYIGGVGLAKGYLHNPKLTTEKF"
     CDS_motif       219..284
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOD08_RS21115_0002"
                     /evalue="1.20E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="MOD08_RS21115"
                     /protein_end="93"
                     /protein_start="71"
                     /score="36.5"
                     /tool="antismash"
                     /translation="FVGRKDHQIKMRGFRIEPQEIE"
     aSDomain        579..779
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOD08_RS21115_PCP.1"
                     /evalue="3.40E-18"
                     /label="MOD08_RS21115_PCP.1"
                     /locus_tag="MOD08_RS21115"
                     /protein_end="258"
                     /protein_start="191"
                     /score="57.6"
                     /tool="antismash"
                     /translation="EKILYEIWKDVLKTEGMDINDDFIQVGGHSINAVQIMSRLQKKGI
                     KLTIDDLYKYKTIKRLSNMLTA"
     aSDomain        1212..1766
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOD08_RS21115_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="8.00E-14"
                     /label="MOD08_RS21115_Condensation_LCL.1"
                     /locus_tag="MOD08_RS21115"
                     /protein_end="587"
                     /protein_start="402"
                     /score="43.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: SHLKFDK"
                     /tool="antismash"
                     /translation="FNEICEISALKKAITIFVEKQELMRSMLRNRDGEYYWDVYSCEEH
                     INIPFLDISEYNLDSQKELLKDILPKMFFSEYSNSLFYRILIIKVNEKEHILLFPFSHL
                     KFDKMSSILIEKEIKSIYNSIEKNESLPTHNINLYSDYVKQVMKGPQLISDQEVNNIYD
                     LNKMVSLHELISVPSNETRPVI"
     gene            2341..>2573
                     /locus_tag="MOD08_RS21120"
                     /old_locus_tag="MOD08_21070"
                     /pseudo=""
     CDS             2341..>2573
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MOD08_RS21120"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MOD08_21070"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKNYNMAATKVEKADNFNQNFVLFSLSRLISELCSSVFKFALSLF
                     VLDLTGSAVLFSTVLGFSILPGVFINIFAGVL"
     assembly_gap    2574..2660
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2661..3638
                     /locus_tag="MOD08_RS21125"
                     /old_locus_tag="MOD08_21075"
     CDS             <2661..3638
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /locus_tag="MOD08_RS21125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD08_21075"
                     /product="MFS transporter"
                     /protein_id="WP_268519686.1"
                     /transl_table=11
                     /translation="SLYFYSDSLTMIIIFTVCLSVVQALFSLTLNSAIPNLVPQDKVTT
                     LNSSYQGIGALINILGPILGALFYKIFGLNIILLISSFAFFVASFLQIFMKFEKPKDIP
                     VLSSYKESFKEVYQYLQKRYVVKYLLVFVVIINFVLTPLLSVVLPFISYQVLDLSASQL
                     SWIQASWAIGFILGAALVSIKKVSNLAANKIFILLQIQSLFFLLWNFPVFPFLGGQSNY
                     IITLIFCAILSISGIFNALANIPMLSYMQVYLPDHLRAGVLGVVNTAVQVAVPLGIWIY
                     GLSLEYQHWSIVITVSSISLFIIGFIANQIRSLKNFFVPITEKN"
     misc_feature    2692..2706
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: strong, score: 25.64"
                     /tool="antismash"
     gene            3722..4093
                     /locus_tag="MOD08_RS21130"
                     /old_locus_tag="MOD08_21080"
     CDS             3722..4093
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899624.1"
                     /locus_tag="MOD08_RS21130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD08_21080"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_268517007.1"
                     /transl_table=11
                     /translation="MPSLNDKLPIFEQISKIIEDDILATVYKEGDQIISTTQISKVFQV
                     NPATAVKGINLLVDQDILYKKRGIGMFVQNGAKARILQKRRQEFYDLFIVKLLNEAKKI
                     ELSNEDIISLIKGESSDNN"
     gene            4105..4965
                     /locus_tag="MOD08_RS21135"
                     /old_locus_tag="MOD08_21085"
     CDS             4105..4965
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="MOD08_RS21135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD08_21085"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_268519687.1"
                     /transl_table=11
                     /translation="MCNNLTKSYKKEKIVKQIQLKFKENSICGLLGRNGSGKTTILNMI
                     SGHSFYDKGNIYVKGKLLLKGCIHQDICYVKDRQHFFWNQTVMEVLKIASSFYQNWDWK
                     AASALLEEFSLDPAIKCRKLSRGMETMVGIIIGMASRAPITIFDEPDAGLDSVMREKFY
                     SLLLEDYTEYPRTIIISTHLVDECAKLLEKIYVIDSGEVILEENIDELRSKMYSLYGER
                     DQIDRFIENKDVLHVESVGNKKIVNIFHPLSSEDIYNLEKSDVSVGNLSVQHFLTQLLE
                     RKKNH"
     gene            4962..5645
                     /locus_tag="MOD08_RS21140"
                     /old_locus_tag="MOD08_21090"
     CDS             4962..5645
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MOD08_RS21140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MOD08_21090"
                     /product="hypothetical protein"
                     /protein_id="WP_268517009.1"
                     /transl_table=11
                     /translation="MNQLSGICKISFTSIRLFLYVFLSLECIHFIINISLAAFGINTHT
                     EVSHANTAAAFLLIIPVILPTFTFKQALFLGASRSSYFWGTIIFLILASCIAALLNTVW
                     YYVEIHILSAYKHYFNIIRIFEWNKSGTMGIFLYQFFAYLPAMAFVHLLFSGFWSRKGI
                     ALWLVSGAVISVSVSVGMLRQCIGLALQFLLYRSHLFISMGCELLLTAAFFFTCWHLFI
                     KQRQV"
     gene            5653..>7782
                     /locus_tag="MOD08_RS21145"
                     /old_locus_tag="MOD08_21095"
     CDS             5653..>7782
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /locus_tag="MOD08_RS21145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOD08_21095"
                     /product="FtsX-like permease family protein"
                     /protein_id="WP_268519688.1"
                     /transl_table=11
                     /translation="MRIIIKSILKSILNKKFRALLILISIIVSAGLFFASISISGTLTD
                     NYREKLKQTAGSSDLVIAVRQNQSKPFLDPVNIKSLQDNFSYTTEEIQTEGFFQGSNAK
                     KEKSSWLNLHGAPIKDINKTNPFNLADSMRGEFKGKKIIVSKKMASENHLKIGDLLTLE
                     IAGQVHHLKIYGIAEASGIFLNEEKSMSAIIPRKTLSHMIGADSKVNVIHIKLKPHVSQ
                     EKMISKLQALYKNASVKETISESDVSNQVGVLPVVLSIMVVVVSFMTIFIIYTCFKVVA
                     LERMSLIGTVRSIGLSRKKANFLLLSESLIYGVIGGGAGAALGIGFLYGITNIASSGTF
                     KVSMHIQITHVLTAILFAVLLCIGSSFIPILKISNIPIKAIILKQTEHNKTNHAVKFIV
                     GVIFIVMSFIISGLTDGKSAVIAVGLSTVLCGVSLLFIVPVMTRVIALLFEKMFSFFSM
                     NILALAAKNLRENKSVLNSISLLTIGITCLIFIQSASTSIMTATSNIYSTTADFDLQMQ
                     AAGSNQSIMKKTRAVEGVDDVFGNYQLLNVEVKNKGAIQTVDSADQKHYLDYWKMNISK
                     KLLYEKLHEQRNILLGTASMEKLGVKQGDMITLVFPQGEYKYKVIGSFDTLYSTGDFAL
                     ISQKYMKRDGGFSTYSNFYVKANHNASQIEKDIQKELRKNKPSISTIKDIKDGYMKSNE
                     NMFLILKAFALFSMVI"
ORIGIN
        1 acaactcaca ggtctatatc ttagataaaa ataagaactt gcttccccca ggcgtaattg
       61 gatgtatata tataggggga gttggattag ccaaggggta tttacataat ccaaagttaa
      121 caactgagaa atttattgat aacccctaca taaaaggaga aaaaatgtat tgtacgggtg
      181 acctggctaa gtggtcagct gatgggaata tagaatttgt cggacgtaaa gaccaccaaa
      241 taaaaatgag gggattccgt atagaacctc aagaaataga gtatcagatc atgcaacata
      301 gagatataaa agaagcagct gtgatcgata tagtgctaga agacaattct aagcagctat
      361 gggcttttat cgtttcaaga gaggaaaatg atagtcagct gttgcgagat tttttgcaag
      421 ataaattacc agattatatg attcccattc gcttttatag tttgaaacgt ctccctttaa
      481 cttcaaatgg caaaattgat cggaattctt tgaaagaaaa atataaaaag gctatccatg
      541 aacggcacaa aatagaaaaa cctaccaaag aaatggaaaa aattttatat gagatttgga
      601 aagatgtttt aaaaacagag ggcatggaca ttaatgatga ctttattcaa gttggaggtc
      661 attctattaa tgcagtgcaa atcatgtctc gattacaaaa gaaagggatt aaactcacca
      721 tagatgatct ttataaatat aaaactatca aaagattaag taatatgctg acagcgagca
      781 gtgtctccaa taagatttat aacatttatg atgctgagaa attgctgaga aaagagtgtg
      841 atctaaatgt atacataaaa agaataaatg ttaaaaacaa aactagatat atttattttg
      901 ttgataatca tatagcaaaa gaaaatgaag tacttgcagc cattaaaaaa tatataagtc
      961 cagaaattca accacattat attaaattca ataaaaataa tgttgatttt ggtcacgaaa
     1021 agtggaatga gatcttagaa ctcaaaagtt taacgaaaga tgaggttaat tttataactg
     1081 atagttgtgt aaataacaat caactttcag ctcattttag aaaaggggaa gtgattaaga
     1141 catttccgct atcaccaata caaagatatc atttagatta tagtgattat tcaggtacaa
     1201 ttgtcttttt taatgaaata tgtgagattt ctgcgcttaa aaaagcgatt actatctttg
     1261 tggaaaagca agaacttatg agaagtatgc tgcgaaacag agatggagaa tattattggg
     1321 atgtatattc gtgtgaagaa catataaata tcccgttctt agatatttct gaatacaatt
     1381 tggactctca aaaggaactg ctgaaagaca tattacccaa aatgtttttc agtgaatata
     1441 gtaattcatt attctatcga atattaatta ttaaagtgaa tgaaaaagaa catattctat
     1501 tgtttccttt ctctcatcta aagtttgata aaatgagcag tattttaata gaaaaagaga
     1561 taaaaagtat ttacaattct atcgaaaaaa atgaatcact gccaactcat aatattaatt
     1621 tgtattcaga ctatgttaaa caagtgatga aaggacctca attaataagt gatcaagaag
     1681 taaataatat atatgattta aataaaatgg tttctttaca tgaactgatt agcgttcctt
     1741 caaatgaaac tagacctgtt atttatacct taagaaaaaa aatagatcat acacaaaatc
     1801 atttagaaac atacctgcag atatgctgtt ctgtactaag taagatgttg ggaattaaag
     1861 atgttcctat atttatcatc caacatggaa gaaatactca agatgggaat tattttggaa
     1921 cattaggtga gttcattgat atttttcctt ttatatggtt tgagacagat actattgaat
     1981 ctgttaacaa tcgtttaaat acgaggattg ctataaagaa aaataaaaat ataaactttg
     2041 ccgcgctctt ttatcagcaa gatataaaaa gcaactttcc aaaaacaaat cgaaatataa
     2101 ccaaaatgtt aagcgaatat cctatcctgt ttaattatct tgatgaaacg aaggaatctg
     2161 ataatgagta taatcagcta atgaaaagta acacaccttt aaacacaggt aagagaatta
     2221 tagttgaggt taacaaaaat gaggaacact tgaatttcac tttcatcttg cctttccgat
     2281 taaaagaaat tacatgtgat ggtgtaattg aatcactcgt tgctcagcaa ggagatacta
     2341 atgaaaaatt ataatatggc agcaacaaag gtagagaaag cggataattt caatcaaaat
     2401 tttgttttat tctcactttc acgattaatc tctgaactat gttcttcagt atttaaattt
     2461 gctttaagtt tatttgtctt agatttaact ggctctgcag ttcttttttc aactgtgttg
     2521 ggtttttcta ttttgcctgg agtttttatc aatattttcg ccggtgtatt agtnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn tctttatatt tctattcaga cagccttact atgattatca
     2701 ttttcactgt ttgtctgagt gttgttcaag cattgttttc gttaacgctt aattcggcga
     2761 ttcctaatct ggtacctcaa gataaagtca ccacattaaa ttcaagttat caaggcatag
     2821 gtgctttaat taatatatta ggaccgattt taggagcgtt gttttataaa atatttggat
     2881 taaatataat tttattgatc agcagttttg ctttctttgt agcatctttt ctgcagattt
     2941 ttatgaaatt tgagaaacct aaagatatcc ctgttttaag cagttataaa gaaagcttca
     3001 aagaggttta tcaatatttg caaaaacgtt atgttgttaa atatttattg gtttttgtgg
     3061 tgatcattaa ttttgtacta actccattat taagtgtggt acttccgttc atttcatatc
     3121 aagtcttaga cttatctgcg agtcaattat cttggataca agctagttgg gcaataggtt
     3181 ttattttagg cgctgcacta gtatccataa aaaaagtgag caatttggcg gctaataaaa
     3241 tatttatttt attacaaata caatcattat tttttctttt gtggaatttc cctgtttttc
     3301 cgttcttagg cggacaatcc aactatatca tcaccctgat tttttgtgcc attttaagta
     3361 tttctggaat atttaatgct cttgcgaata ttcctatgct ctcatacatg caagtgtatc
     3421 tgcctgatca tttaagagct ggagtactcg gagtagtaaa tactgctgtg caagtcgcag
     3481 tacctttggg gatatggata tatggtcttt ctttagaata tcagcattgg agcatcgtaa
     3541 ttactgtctc tagcatctct ttatttatta ttggatttat tgcaaaccag attaggtctt
     3601 taaaaaattt ctttgttcca ataacagaaa aaaattgaca aaatattgtt tttgctatac
     3661 ggttaattac atgagtaact aaccgtatag gttctgcctg tgagagaaag gaaaggtgtg
     3721 gatgccgagt ttaaatgata aactgccgat ttttgagcaa atttctaaaa ttatagaaga
     3781 tgatatcttg gcaacggttt ataaagaagg ggaccaaatt atttccacaa ctcaaatatc
     3841 caaagtattc caagtgaatc ctgcaacagc agtgaaaggc attaatttgc tggttgatca
     3901 ggacattctt tataaaaaaa gaggtattgg aatgtttgtt caaaatggtg ccaaagcaag
     3961 aattcttcaa aaaagaagac aagagtttta tgatttattt attgtaaaac tattaaatga
     4021 agccaaaaaa atagaattgt caaatgaaga cattatctca ttaattaaag gtgaaagtag
     4081 tgataataat tgaaggatat tcttgtgtgc aataatttga caaaatcata caagaaagaa
     4141 aaaatcgtta aacagattca attgaaattt aaggagaatt ccatctgcgg gcttctcggt
     4201 agaaatggtt cgggcaaaac gactatatta aatatgataa gcggtcatag tttttatgac
     4261 aaagggaata tttatgtaaa gggaaagttg cttttaaaag gctgtatcca tcaagatatt
     4321 tgttacgtaa aagacagaca gcattttttt tggaatcaaa ccgtaatgga agtgctaaaa
     4381 attgcttctt cgttctatca gaattgggat tggaaggcag cttctgcttt gcttgaagag
     4441 ttttcacttg atccggcaat aaaatgcaga aagctttcca gaggtatgga aacgatggtc
     4501 gggatcataa taggaatggc cagcagagcg cccattacta tttttgatga gcctgatgca
     4561 ggattagatt ctgtcatgag agaaaagttt tacagtctat tactcgaaga ttatacagaa
     4621 tatccccgca cgattattat atcaactcat ttagtagatg aatgtgcaaa acttcttgaa
     4681 aaaatttatg ttattgattc aggagaggtt attcttgaag agaatataga tgaactaaga
     4741 agtaaaatgt attcattata tggggaaaga gaccaaatag accgatttat tgaaaataaa
     4801 gatgtactgc atgttgaatc tgtaggtaat aaaaaaatag ttaatatttt tcatcctctg
     4861 agttcggaag atatatataa tttagaaaaa tcagatgttt cagtcgggaa tttgtcagtg
     4921 cagcattttt taactcagct gcttgaaagg aagaaaaatc attgaatcag ttgtccggta
     4981 tctgtaagat cagctttact tctataaggt tgtttttata tgtgttttta tctttggaat
     5041 gtattcattt tataatcaat attagtttag ctgcatttgg aatcaatact catacagagg
     5101 tttcgcatgc taatactgca gctgctttcc tgcttatcat tccggtaatc ttgccaacct
     5161 ttacgtttaa acaggcgctt tttttagggg cttccagatc atcatatttt tggggaacga
     5221 tcatattttt gattttggct tcctgcatag ctgcattatt gaatacagtt tggtattacg
     5281 ttgagattca tattttaagt gcttataaac attactttaa cattatccgc attttcgaat
     5341 ggaataagtc aggaacaatg gggatatttc tatatcaatt tttcgcatat cttcctgcta
     5401 tggcttttgt gcacttatta ttctccggct tttggagcag aaagggaatt gccctgtggc
     5461 ttgtatcagg cgctgtcatt tctgtaagtg tgtcagtagg catgctgaga caatgtatcg
     5521 gtttggcgct tcagttttta ctttaccgct cacatttatt tataagcatg ggatgtgaat
     5581 tgttgctgac agccgcattc ttcttcacat gctggcactt atttatcaaa caaaggcagg
     5641 tttaatgtat acatgcgtat tattataaag agtatcttaa aatctatttt aaataaaaaa
     5701 ttccgcgctc tgcttattct tatatctatt attgtatctg ctggtctttt ctttgcatct
     5761 atctccattt caggaaccct tacggacaat tacagagaaa aattaaaaca aacagcagga
     5821 agttcagatc tagtgattgc ggtccgtcag aaccaatcaa aaccattctt agaccctgtt
     5881 aatataaaat ctcttcaaga taatttttct tatacgacag aggaaattca aacagaaggt
     5941 ttttttcaag ggtctaatgc aaagaaagaa aaaagttcct ggctcaactt acacggagca
     6001 cctatcaagg atattaataa aacaaacccg tttaatctag ccgactcaat gcgaggagaa
     6061 tttaaaggga aaaaaataat cgtcagcaaa aaaatggcga gcgaaaatca tttgaaaata
     6121 ggcgatcttc ttacactaga aattgcgggt caggttcatc atcttaaaat atacggtata
     6181 gcagaagctt cgggaatttt tctgaatgag gaaaaatcca tgtctgccat tattcctcgg
     6241 aaaacgctct ctcacatgat cggagcggat tcaaaagtga atgtcataca tattaaatta
     6301 aagcctcatg tgagccaaga aaaaatgatt agtaagcttc aggctcttta taaaaatgct
     6361 tcggttaaag aaaccatttc agaatctgat gtaagcaatc aggtcggtgt tctgcctgtt
     6421 gttctctcta tcatggttgt cgttgtttct tttatgacta tatttatcat atatacatgc
     6481 tttaaagttg tggctcttga aaggatgtct cttatcggaa cggttagaag cattggcctg
     6541 tcaagaaaaa aagcaaattt tcttctgctt tcagagagct tgatatatgg agtaataggc
     6601 ggtggagccg gagctgcttt agggattgga tttttgtatg gcattactaa tatagcctcc
     6661 tcgggtacgt tcaaagtgtc tatgcatatt caaattactc acgtactaac tgccatattg
     6721 tttgcagtgc tgttatgtat agggagctcg ttcataccga tcttgaaaat ttccaatatt
     6781 ccaattaaag caataatact taaacaaaca gaacacaata aaacaaatca tgcagtcaaa
     6841 tttatcgtag gcgttatttt cattgtgatg agttttatta tatccggact gacagacgga
     6901 aagtcagctg taattgctgt cggtctctca acggttcttt gcggtgtctc tttacttttt
     6961 attgttcccg tcatgacacg ggtgatcgca ctgctatttg agaaaatgtt cagctttttc
     7021 tcaatgaata ttctagcgct tgctgcgaaa aatttaagag aaaataaaag tgtcctgaat
     7081 tccatttcac ttttgacaat tggtattact tgtttaattt ttattcagtc tgccagtacg
     7141 agtattatga cggcaacatc caacatttac agcactaccg cggactttga tctgcaaatg
     7201 caagctgctg ggagcaatca atctattatg aaaaaaaccc gtgctgtaga aggtgtggat
     7261 gatgtgtttg ggaattacca actactaaac gttgaggtga aaaataaggg agccattcaa
     7321 acggttgaca gtgctgatca aaagcattac ttagattatt ggaaaatgaa catcagcaaa
     7381 aaattactat acgaaaagct ccatgaacag cgaaacattt tattaggaac agcttcgatg
     7441 gaaaaactcg gagtgaaaca gggagatatg atcactttgg ttttcccgca aggcgaatat
     7501 aagtataaag taatcggtag ctttgacacc ttatactcca ctggtgactt tgctcttatt
     7561 tcacagaagt atatgaaacg ggatggcggc ttcagcacct attcaaattt ctatgtgaaa
     7621 gctaatcata atgcctcgca gatagaaaaa gatattcaaa aagagcttcg aaaaaataaa
     7681 ccaagcattt ctactataaa ggatataaaa gatggttata tgaaaagtaa cgagaatatg
     7741 tttctcattc taaaagcttt tgctttattt tccatggtga ta
//
