LOCUS       NZ_JALAPZ010000025      8834 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain CK3O2B-55B contig_0000025, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAPZ010000025
VERSION     NZ_JALAPZ010000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203.06051644694466x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 01:56:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,214
            Genes (coding)                    :: 4,095
            CDSs (with protein)               :: 4,095
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 71 of 119
            Pseudo Genes (incomplete)         :: 75 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 39 of 119
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:03:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8834
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAPZ010000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..8834
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3468](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3468)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..3468)
                     /locus_tag="MOC81_RS21400"
                     /old_locus_tag="MOC81_21355"
     CDS             complement(<1..3468)
                     /NRPS_PKS="Domain: Condensation_Starter (3-300). E-value:
                     1.6e-89. Score: 292.1. Matches aSDomain:
                     nrpspksdomains_MOC81_RS21400_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (463-864). E-value:
                     2.1e-112. Score: 367.7. Matches aSDomain:
                     nrpspksdomains_MOC81_RS21400_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (966-1035). E-value: 2.3e-26. Score:
                     83.8. Matches aSDomain: nrpspksdomains_MOC81_RS21400_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOC81_RS21400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_21355"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268309110.1"
                     /sec_met_domain="Condensation (E-value: 2.4e-75, bitscore:
                     241.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.7e-123, bitscore:
                     400.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.2e-17, bitscore:
                     51.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQHTYSLTHAQRRVWFTELLEPDTSICNLTACVKFRGNIDLDVL
                     EGALNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQA
                     RIPFKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDP
                     LPDQPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSAS
                     RDTITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKA
                     EKEMLGMFVSSLPIRITVDPDADFLSFVRRVGREQLSVMRHQRFPYNLLVNELRKEQKD
                     LHNLIGISMQYQPLQWHNADGFDYETALYFSGYTANELSVQIQERTDTGTIQLNFDYQN
                     TLFSLEDIKRIQNHLLTLLENALQHPHSFIKELDMTNIREKQKLLYEFNKTEAVSPEAP
                     TLHGLFERQAAVTPERPAIRFSSGSLTYAELDMYASRLAARLAARGVTKESIVGVLSER
                     SPEMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDSGAALLLAQPGCSAPSFSGEMLE
                     VDMASLASEKAENDVFTPADGCSLAYVIYTSGSTGKPKGVAVEHRQAVSFLTGMQRQFP
                     LQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAIHQEKVTTAH
                     FIPAMLNSFLDQADMKALGDGTSLKRVFAGGEPLAPHVAARFASLLPQVSLIHGYGPTE
                     ATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDAHLAVQPCGVAGELYIAGAGVAR
                     GYLNRPALTEERFLEDPFYPGERMYKTGDVARWLPDGHVEFLGRLDDQVKIRGYRIEPG
                     EIEAVLRGIEGIREAAVTVRTDSGEPELCAYTEGLRRNEVRTQLERLLPSYMIPAHMIE
                     MERWPVTPSGKLDRNALPAPGGAADAETYTAPSNVTEMKLAQLWEDVLKNGPVGIHDNF
                     FDRGGHSLKATALVSRIAKEFDVQVPLKDVFAHPTVEGLASVIREGTDSPYEAMKPAEK
                     REAYPVSSAQKRIYVLQQLEDGGTGYNMPAVLELEGKLDPERLDRAFKELIKRHESLRT
                     SFEQDEGGEPVQRIHDVVPFTLQTAVLGEQTEQEAAAAFIEPFDLSQAP"
     source          1..8834
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="CK3O2B-55B"
                     /submitter_seqid="contig_0000025"
     region          1..8834
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..8834
                     /SMILES="NC(CCC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(142..249)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0011"
                     /evalue="3.00E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="1109"
                     /protein_start="1073"
                     /score="58.2"
                     /tool="antismash"
                     /translation="YNMPAVLELEGKLDPERLDRAFKELIKRHESLRTSF"
     CDS_motif       complement(280..315)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0010"
                     /evalue="3.60E+00"
                     /label="C1_starter_004-017"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="1063"
                     /protein_start="1051"
                     /score="1.6"
                     /tool="antismash"
                     /translation="YPVSSAQKRIYV"
     aSDomain        complement(364..570)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOC81_RS21400_PCP.1"
                     /evalue="2.30E-26"
                     /label="MOC81_RS21400_PCP.1"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="1035"
                     /protein_start="966"
                     /score="83.8"
                     /tool="antismash"
                     /translation="EMKLAQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFD
                     VQVPLKDVFAHPTVEGLASVIREG"
     aSModule        complement(364..3459)
                     /complete
                     /domains="nrpspksdomains_MOC81_RS21400_Condensation_Starter
                     .1"
                     /domains="nrpspksdomains_MOC81_RS21400_AMP-binding.1"
                     /domains="nrpspksdomains_MOC81_RS21400_PCP.1"
                     /locus_tags="MOC81_RS21400"
                     /monomer_pairings="Glu -> Glu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(847..912)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0009"
                     /evalue="6.10E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="874"
                     /protein_start="852"
                     /score="40.5"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
     aSDomain        complement(877..2079)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOC81_RS21400_AMP-binding.1"
                     /evalue="2.10E-112"
                     /label="MOC81_RS21400_AMP-binding.1"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="864"
                     /protein_start="463"
                     /score="367.7"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="FERQAAVTPERPAIRFSSGSLTYAELDMYASRLAARLAARGVTKE
                     SIVGVLSERSPEMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDSGAALLLAQPGCSA
                     PSFSGEMLEVDMASLASEKAENDVFTPADGCSLAYVIYTSGSTGKPKGVAVEHRQAVSF
                     LTGMQRQFPLQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAI
                     HQEKVTTAHFIPAMLNSFLDQADMKALGDGTSLKRVFAGGEPLAPHVAARFASLLPQVS
                     LIHGYGPTEATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDAHLAVQPCGVAGEL
                     YIAGAGVARGYLNRPALTEERFLEDPFYPGERMYKTGDVARWLPDGHVEFLGRLDDQVK
                     IR"
     CDS_motif       complement(994..1083)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0008"
                     /evalue="8.90E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="825"
                     /protein_start="795"
                     /score="56.3"
                     /tool="antismash"
                     /translation="PCGVAGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       complement(1609..1668)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0007"
                     /evalue="3.20E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="620"
                     /protein_start="600"
                     /score="34.9"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVAVEH"
     CDS_motif       complement(1852..1893)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0006"
                     /evalue="7.30E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="539"
                     /protein_start="525"
                     /score="19.0"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPLD"
     CDS_motif       complement(2416..2529)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0005"
                     /evalue="2.40E-09"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="351"
                     /protein_start="313"
                     /score="29.7"
                     /tool="antismash"
                     /translation="REQLSVMRHQRFPYNLLVNELRKEQKDLHNLIGISMQY"
     aSDomain        complement(2569..3459)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOC81_RS21400_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="1.60E-89"
                     /label="MOC81_RS21400_Condensation_Starter.1"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="300"
                     /protein_start="3"
                     /score="292.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="HTYSLTHAQRRVWFTELLEPDTSICNLTACVKFRGNIDLDVLEGA
                     LNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQARIP
                     FKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDPLPD
                     QPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSASRDT
                     ITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKAEKE
                     MLGMFVSSLPIRITVD"
     CDS_motif       complement(2581..2670)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0004"
                     /evalue="1.60E-08"
                     /label="C5_starter_265-294"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="296"
                     /protein_start="266"
                     /score="27.0"
                     /tool="antismash"
                     /translation="DLAIGTYYGNRGSKAEKEMLGMFVSSLPIR"
     CDS_motif       complement(3004..3069)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0003"
                     /evalue="1.20E-07"
                     /label="C3_starter_134-155"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="155"
                     /protein_start="133"
                     /score="24.6"
                     /tool="antismash"
                     /translation="FHHIIMDGISLNVMGNQIIDLY"
     CDS_motif       complement(3274..3390)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0002"
                     /evalue="8.40E-10"
                     /label="C2_starter_024-063"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="65"
                     /protein_start="26"
                     /score="31.3"
                     /tool="antismash"
                     /translation="ICNLTACVKFRGNIDLDVLEGALNHSISRNDAIRFQLLE"
     CDS_motif       complement(3415..3453)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOC81_RS21400_0001"
                     /evalue="7.30E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="MOC81_RS21400"
                     /protein_end="18"
                     /protein_start="5"
                     /score="9.4"
                     /tool="antismash"
                     /translation="YSLTHAQRRVWFT"
     misc_feature    complement(3508..3524)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 22.38"
                     /tool="antismash"
     gene            complement(3839..5317)
                     /gene="dacB"
                     /locus_tag="MOC81_RS21405"
                     /old_locus_tag="MOC81_21360"
     CDS             complement(3839..5317)
                     /EC_number="3.4.16.4"
                     /GO_function="GO:0004175 - endopeptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241941.1"
                     /locus_tag="MOC81_RS21405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_21360"
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="WP_268292946.1"
                     /transl_table=11
                     /translation="MKKSIKLYVAVLLLFVVASVPYMHQATQAAEKQGALAGQIDKILA
                     DHPALEGAMAGITVRSAETGAVLYEHSGEIRMRPASSLKLLTAASSLSVLGEDYSFTTE
                     VRTDGKFKGKKLNGNLYLIGKGDPTLLPSDFEEMAEKLKQSGVKEIKGNLIGDDTWYDD
                     IRLSPDMPWSDEYTYYGAQISALTASPNEDYDAGTVIVEITPGKKEGEKPAVSVSPKTD
                     YVTIKNDAETTAAGQKKDLTIEREHGTNTITIEGSVPVDASKTKEWIAVWEPAGYALDL
                     FKQALKKQEITVKGHVKTGTAPGSSDVLISHRSMPLSKLFVPFMKLSNNGHAEVLVKEM
                     GKVKKEEGSWEKGLEVLNNALPEFGVDPKSLVLRDGSGVSHINAVSSDQISQLLYDIQD
                     EKWFSAYLNSLPVAGNSDRMIGGTLRNRMKDTPAQGKVRAKTGSLSTVSSLSGYAETKS
                     GKTLVFSILLNGLIDDEDGKDIEDQIAVILANQS"
     gene            complement(5352..6329)
                     /gene="galM"
                     /locus_tag="MOC81_RS21410"
                     /old_locus_tag="MOC81_21365"
     CDS             complement(5352..6329)
                     /codon_start=1
                     /gene="galM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389718.1"
                     /locus_tag="MOC81_RS21410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_21365"
                     /product="aldose 1-epimerase"
                     /protein_id="WP_225516923.1"
                     /transl_table=11
                     /translation="MANDIEKITYLGTPAIKAGNEHIEMIVVPEWGSNVISLVDKTTQL
                     QLLREPETAESFHDTPTLYGIPILFPPNRISDGTFSFRGRTYHFDINEKDKHNHLHGFL
                     YQEKWNVVTMKQTDEEVIVETEIDLSELPHVYKQFPHHAVVRMTYTLKGNTLFKHATVM
                     NKGKEAFPWGIGYHTTFVFPEESSLFSLTADQQWELDERLLPTGKLMDVQNKEALHEGM
                     DLRSQQLDDVFLSSYQKRGGENQAVIYHQHAHISIIYKADEQFKHWVVYNADGKQGYLC
                     PEPYTWVTNAVNLNVSPSLTGVQVLEPGEETTAKSSITIELNHQ"
     gene            complement(6462..7853)
                     /locus_tag="MOC81_RS21415"
                     /old_locus_tag="MOC81_21370"
     CDS             complement(6462..7853)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389719.2"
                     /locus_tag="MOC81_RS21415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_21370"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_003241939.1"
                     /transl_table=11
                     /translation="METSQEQLVTQKKSNPSVWRSMSLFLVPLLLSNVLQSVGQLVGMM
                     AVGRWLGVDAVAAVSSFFPLFFLLISFTIGIGSGSSILIGQAYGAKNEERLKAVVGTTL
                     TFTFLLGVVLAVIGSIFTLDILRLMGTPENVIHVSASYARILFYAMPFMFLYFAYTTFL
                     RGTGDSKTPFYTLIVSTVINIALLPVLILGMFGFPKLGIYGSAYATVISTIATFIVLMV
                     YLRKRNHPLQFDKTVRRYLKMDKELLALLLRLGIPSSINMILVSLSEIAVISFVNHYGS
                     NATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANEFGRLKQVIRVGIWLNYIIGG
                     VLIILIYVFSHQILGLFLTEQETLYIAHRLLMITLWSYLLFGNAQIISATMRASGTVLW
                     PTVISIFAIWGVEVPVAFVLSHYTNLEILGVWVGYPAAFAVSLLLIYGYYQFVWKKKQI
                     TRLIQ"
     gene            8140..8685
                     /gene="iseA"
                     /locus_tag="MOC81_RS21420"
                     /old_locus_tag="MOC81_21375"
     CDS             8140..8685
                     /codon_start=1
                     /gene="iseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389720.2"
                     /locus_tag="MOC81_RS21420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_21375"
                     /product="DL-endopeptidase inhibitor IseA"
                     /protein_id="WP_003241937.1"
                     /transl_table=11
                     /translation="MKKCFLFLTTIALILSLSTNAFAKNTSGDLSQKQALQLALSAREH
                     FWNTMSGHNPKAKKAVCPSGTFEYQNLQYVYMCSDLGTKAKAVNYLTPIFTKTAIEKGF
                     KDYHFTVSKGKLAVPIGDGDNLLNWKKSTAKLISKKGGTVTYEFTVPTLDGSPAAKRKV
                     TFVKENKKWKVNQFDAVI"
ORIGIN
        1 cggcgcttgg ctgagatcaa agggctcgat aaacgcggct gcggcttcct gttcggtttg
       61 ttcgccgagg actgctgtct gtaatgtaaa cggcaccacg tcatggatgc gctgcaccgg
      121 ctcgccgcct tcgtcctgtt cgaaggatgt ccgaagcgac tcgtggcgct tgattagctc
      181 tttaaaggcc ctgtccagcc tttcggggtc cagcttccct tccagctcta acacagccgg
      241 catattgtag cctgtccctc cgtcctccag ctgctgaagg acatagatcc gtttttgagc
      301 ggaggaaacc ggataggcct cgcgtttttc agccggcttc atcgcttcat atggactgtc
      361 cgttccttca cggataacgg aagccagtcc ttcaacagtc ggatgggcaa acacatcttt
      421 cagcggcacc tgtacatcga attctttggc gatccgggag acaagcgccg ttgcttttaa
      481 ggaatgtcct ccgcggtcga aaaagttgtc gtgaatcccg acaggaccat ttttcagcac
      541 gtcctcccac agctgggcaa gcttcatctc tgtcacattg ctcggcgccg tgtaggtctc
      601 cgcatctgca gctccgccag gagctggcag ggcgtttcgg tccagctttc cgcttggcgt
      661 aaccggccac cgttccatct ctatcatatg ggctgggatc atgtagctcg gcagcagtct
      721 ttcaagctgc gtccgcactt cgtttcttct tagtccttct gtataggcgc ataattccgg
      781 ctcgccgctg tctgtccgta cggtaacggc tgcctctctg atgccctcta tgcctcttag
      841 caccgcttca atttctccgg gctcaatccg gtagccgcgg attttcacct gatcatccaa
      901 acggccgagg aattcaacat gtccgtcagg aagccagcgc gccacatccc cggttttgta
      961 catgcgttca cccgggtaaa acgggtcctc aagaaaacgc tcctccgtta aggccggccg
     1021 attcaaatag cctctggcca cacccgctcc ggctatgtac agttcgccgg cgacaccgca
     1081 aggctgtacg gctaaatgcg catctaaaac atacaaacgc gcaccgggta cgggcttccc
     1141 gatcggaatc cgcaagcgat ccctgtcccg ctctgggtcc aacacgtaaa atgcagcgtc
     1201 aaccgttgct tctgtcggcc cgtagccatg aatcagcgat acctgcggca ataaagaagc
     1261 aaaacgggct gccacatggg gcgcaagcgg ttcacctccg gcgaatacac gcttcaggct
     1321 tgtcccatca ccgagcgctt tcatatccgc ttgatcgaga aagctgttca gcatagccgg
     1381 aataaaatga gccgttgtca ctttttcctg atgaatggct tttacaatca atgccgggtc
     1441 tttctcccag ccgggcggaa gcagataagc cgaagcgccg gaaagggacc accaaaacag
     1501 ctgccacaca gacgcatcaa aggaaaaaga ggttttcacc ataacaatgt catcttcctg
     1561 aagcggaaac tgacgctgca tgccagtcag aaaggaaaca gcctggcggt gctcgacggc
     1621 cactcctttt ggcttcccgg tggagcccga tgtatagatg acataggcga gagaacagcc
     1681 gtcagcagga gtaaacacat cgttttctgc tttttcgctc gcaagagatg ccatgtccac
     1741 ttcaagcatc tctccggaaa agctcggtgc ggaacatccc ggctgtgcca gcaagagcgc
     1801 ggccccgctg tctttcagca tgtagctcag ccgctctttt ggatatgcag gatcaagcgg
     1861 caaatacgcc ccgcctgcct tcaatacagc aagtaccgct atcagcattt caggtgaccg
     1921 ttcagacagg acaccgacga tgctttcctt cgtgacgccg cgtgccgcga ggcgcgcagc
     1981 cagccggctg gcatacatgt caagctcagc gtacgtcagc gaaccgctgg aaaagcggat
     2041 ggctggccgc tccggtgtga cagccgcctg tcgttcaaac agaccgtgca gggttggggc
     2101 ttcaggcgaa acagcttccg tcttattaaa ctcatacaac aatttttgtt tctctcttat
     2161 attagtcata tctaattctt ttatgaaact gtgcggatgt tgaagggcgt tttccagtaa
     2221 cgtaagcaga tggttctgta ttcgcttaat atcctcaaga gaaaacaagg tattttgata
     2281 gtcgaaattt aattgaatag ttccggtgtc tgtccgttcc tgtatttgaa cagaaagctc
     2341 gttcgcggtg tatccgctga aataaagcgc cgtttcatag tcaaaaccgt ctgcattgtg
     2401 ccattggagc ggttgatact gcatagatat accgataagg ttgtgtaaat ccttctgttc
     2461 ttttcttaat tcatttacca gcaaattata aggaaaccgt tggtgacgca taacggaaag
     2521 ctgttccctg cctactctgc ggacaaagga tagaaagtcc gcgtcaggat caaccgttat
     2581 tctgatggga agagaactga cgaacatgcc gagcatttct ttttcagcct ttgaccctct
     2641 gttgccatag taggtgccga tggcaaggtc ttttttagaa gtgatccggc tgatgcatat
     2701 gtaaaatgaa gccataaata aggaaatgat attgatcttt tgttcttcgc aaaagattcg
     2761 aatcgtttgc cctagatgag gtgaaagggt gatggtatct ctggatgcgg atgtgctctg
     2821 tttttgtaaa gacgtctggc cggctaacga gtggtatccc ggcggattct gaaaggtttg
     2881 agtccaaaat aaacggtcct ttgtaaaacg tgaagactgt aaatattgac tctctttttc
     2941 gatgtagctc aagtaagaag gttcaggctg atcgggtaaa ggatcttgct ttttcatttt
     3001 ttgataaaga tctataattt gatttcccat cacgtttaag gagatgccgt ccattataat
     3061 atgatggaat ttagcaaaca gccaaacctc atgattgtgg attctaagga gataaaattg
     3121 ataaagaggc gagttaaata gtttaaatgg gattctcgct tgttcttgta tccattgctc
     3181 tatttctatc atttcaacat ttgaaaaatc tattattccc aatgaagtat atttatattc
     3241 agtaaggtac aatcgcggct caagctcttc tccttctagc agctgaaacc tgatggcatc
     3301 gttgcgggag atggaatgat tcagcgcgcc ctctagaaca tcaagatcaa tattgcctct
     3361 gaatttcaca caggccgtaa gattgcaaat gctggtatct ggctccagca gttcggtaaa
     3421 ccacactctc ctttgggcat gggttaaaga ataagtatgt tggctcaacg gattccctcc
     3481 agttctcata ataagagagt aagaaaaaag tagaaaaatg acaataaaag aaattatatg
     3541 actatataat ccatatatgc actatattaa cggatatttt ttaaagtaca ataaaaaata
     3601 ttaaattttt ataacatttt tttgaatata gtattatttc tttagttttg gtttctttct
     3661 tcatcaaatt tttgaacata aagtgataaa agtatcattc tgattcgggt cttacacact
     3721 aatttttcaa ttgtgctaat ttactgttgc gcgtaatctt tctatttgtt ggcagatgcg
     3781 accggaatat tattgaagac ataaaaaaac ctgttttcct tggaaaacag gtctgacgct
     3841 atgattgatt tgccaagatg acagcgattt gatcctcgat atcttttcca tcctcgtcat
     3901 caatcaatcc gttcaaaaga atagaaaaaa caagggtttt tccgctcttg gtttctgcgt
     3961 acccggataa agagctgact gtgcttaacg atccggtttt tgcccttacc tttccttgtg
     4021 caggagtgtc tttcatccgg ttcctcagtg ttccgcctat catcctgtca ctattgcccg
     4081 caaccggcag agaatttaga taagctgaga accatttctc atcttgaatg tcatataaca
     4141 gctgtgagat ttgatctgag gatacagcat taatatggga gacacctgat ccgtctctta
     4201 agacaagtga tttgggatca acaccaaatt ccggaagcgc attgtttaat acttcaagtc
     4261 ccttttccca gcttccttct tcttttttca ctttccccat ctcttttacg agcacttcgg
     4321 catgtccgtt attgcttaat ttcataaacg gcacaaatag ttttgacaga ggcattgagc
     4381 gatgagagat taacacgtct gaagaaccgg gcgcagtgcc tgtcttgacg tgccctttta
     4441 cagtaatttc ttgttttttt agtgcctgct taaataaatc caaagcgtat ccagccggct
     4501 cccagacagc aatccattcc tttgtcttgc ttgcgtcaac cggtacgctc ccttcaattg
     4561 tgatcgtgtt tgttccatgc tctctttcga tcgtgaggtc tttcttttgg ccagccgcag
     4621 tcgtttcggc atcgttctta atcgttacat aatccgtctt cggagataca gaaacggcag
     4681 gcttttcccc ctctttttta cccggggtga tctctacaat cacagtgcct gcatcataat
     4741 cttcattcgg agacgctgtt aaagcggaga tttgcgcgcc gtaatatgtg tattcatcac
     4801 tccacggcat atcaggcgat aatcggatgt catcatacca cgtatcatca ccgatcagat
     4861 tgcctttgat ctctttcacc ccggattgtt tcaatttctc agccatttct tcaaaatcag
     4921 atggtaaaag tgtcggatct cctttcccta tcaaataaag gtttccattc agctttttcc
     4981 ctttaaattt gccgtccgtc cggacctcgg ttgtaaagga atagtcctcg ccaagaacag
     5041 aaagtgatga ggccgctgtc aaaagtttta aagaagaggc gggcctcatt cggatctctc
     5101 ctgaatgctc atatagaaca gcgcctgttt ccgcagaacg aacggtgatg ccggccattg
     5161 cgccttcgag tgcaggatga tcagctaaaa tcttatcgat ctgcccggca agtgcacctt
     5221 gtttttcagc cgcttgcgta gcctgatgca tataaggtac cgaagcaacg acgaaaagca
     5281 gcaaaacagc aacataaagc tttatgcttt ttttcatttt tttaccccgc ctctctgcgc
     5341 ctcatcatgg gctattgatg attcagttca atggtgatac tgcttttggc tgtcgtttct
     5401 tctcccggct ccagcacttg tacaccagtg agtgatggag acacattaag attcacagcg
     5461 tttgtcaccc atgtgtaagg ttcagggcat aaatatccct gctttccgtc cgcgttgtat
     5521 acaacccaat gtttaaattg ttcatctgcc ttataaataa tgcttatatg tgcatgctga
     5581 tgataaatga ctgcctgatt ttctccgccc cttttctgat aagaagataa aaagacatca
     5641 tccagctgct ggctgcgtaa atccatccct tcatgcaagg cttctttatt ctgtacatcc
     5701 atcagtttcc cggtcggcag caggcgctca tccaactccc attgctgatc tgctgtcagg
     5761 gagaataacg agctttcttc tggaaataca aatgtagtat gatagccgat tccccaagga
     5821 aacgcttcct ttcctttgtt catgaccgtc gcatgtttaa atagagtatt cccctttagc
     5881 gtgtatgtca tccgaacgac ggcatgatgc ggaaattgct tatacacatg gggaagctct
     5941 gaaaggtcaa tttcagtttc aacgatcact tcttcatcag tttgcttcat ggtcactacg
     6001 ttccattttt cctgataaag aaacccatga agatggttgt gtttatcctt ttcattgata
     6061 tcaaaatgat atgttctgcc ccggaaactg aatgtaccgt cacttatccg gttaggcgga
     6121 aacaggatcg gaatgccata caatgttggt gtatcatgga agctttctgc tgtttccggt
     6181 tcacgcaaaa gctgaagctg tgtcgttttg tccacaagag aaatgacgtt gctcccccac
     6241 tcgggaacga caatcatttc tatatgttcg ttgcctgctt tgatggcagg tgttcctaaa
     6301 tacgtaatct tctcaatatc gtttgccatg tgtactcaac ctctccttga ttcattgctt
     6361 gtataagaat tcaatgtaat gtgccatatt tcctccattt atgttttctc tttatcaaac
     6421 aaaaaacacc gttcaaatgt tgaacggtgt gattcgagat gctattggat gagacgtgta
     6481 atctgtttct ttttccaaac gaattgataa tacccataaa tcaacagcaa gctgacggca
     6541 aacgcagcag gatagccaac ccagacgccg agtatttcaa gatttgtgta gtgggaaagt
     6601 acgaacgcga caggcacctc tactccccag atggcaaaaa tactgataac ggtcggccat
     6661 aaaaccgttc cgcttgcccg catcgtcgca gaaataattt gggcatttcc aaagagtaag
     6721 taactccaca atgtaatcat caagagacgg tgcgcaatgt aaagcgtctc ttgttcggtt
     6781 aaaaataatc ctaaaatctg atgtgaaaaa acatagatca gaatgatcag cacaccgcca
     6841 atgatataat tcagccaaat cccgacacga attacctgtt ttaggcggcc aaactcgttc
     6901 gctccgatcg attgcgcggc gaatatagac accgcaatac caaggctgac ggccggcatc
     6961 tgcacatagc tggctacctg gttgaccacg ccgtatgcag ctgtcgcatt agagccataa
     7021 tggttgacga atgaaataac cgctatctct gataatgaga cgagaatcat attgatgctt
     7081 gatggaatcc cgaggcgcag caggagagcc agaagttctt tatccatttt caaataacgg
     7141 cgcactgttt tatcaaattg cagcggatgg tttcgttttc gcaaatacac cattaacaca
     7201 ataaaagtcg caatcgtgga aatgacggtt gcatatgcgg agccgtatat gcccagtttc
     7261 ggaaaaccga acatgccaag gatcaagaca ggcaaaagcg ctatgttaat gaccgtgctc
     7321 acaatcagcg tataaaaagg tgtcttagaa tccccagttc cgcgcaaaaa tgttgtataa
     7381 gcaaaatata aaaacataaa cggcatcgcg taaaataaaa tacgtgcata gctggcgctg
     7441 acatgaatta cattttcagg cgtgcccatt aaacgaagaa tatccaaggt gaaaatactg
     7501 ccgatgacgg ctaacacaac ccctaataaa aacgtaaacg tgagcgtcgt cccgacaaca
     7561 gcctttaacc gctcctcatt tttcgccccg taggcttgtc cgatcaggat cgagcttcct
     7621 gaaccgatcc caattgtaaa tgagattaaa agaaaaaata acgggaaaaa ggatgataca
     7681 gctgcaaccg catcaacacc aagccatctg cctacagcca tcatcccgac taactggccg
     7741 actgattgca gtacattgct tagcagaagc ggaactaaaa agagtgacat agaccgccag
     7801 acagatggat tgcttttctt ctgagttaca agttgttctt gtgatgtttc catattgaaa
     7861 gctcctttat ttgaattcgt ttgtaaacat gaccctattc ttttgacaaa aacaggatat
     7921 gtaacatgtt attattcatc actgtacata tgaaaccctt cccgattctt ctaattttac
     7981 acatcttttt agcttatgaa gaataaacta gcaagtaaca gccttttttc ttgtgagtaa
     8041 aagaataaaa aataatttat gcaaaagtat tgtaatctat ccgtaattat tgtaacattt
     8101 gtaacataag agaacgagat tttgaaggag tgaaccacaa tgaaaaaatg ttttctattt
     8161 ctaacaacga ttgcacttat tctgtcatta agcacaaatg catttgcgaa aaatacttca
     8221 ggtgatttat cacaaaaaca agcactgcag ctggcgttat cagcaagaga gcatttttgg
     8281 aacacaatga gcggccacaa tccaaaagcg aaaaaagcag tttgcccatc aggcactttt
     8341 gagtatcaaa atcttcaata cgtgtacatg tgcagtgacc taggcactaa agcaaaagca
     8401 gtgaattact taactcctat tttcacaaaa acagcaatcg aaaaaggctt taaagactat
     8461 cacttcactg tttcaaaagg aaaactagcc gtgccaatcg gcgacggtga caacctttta
     8521 aattggaaaa aatcaactgc taaactgatc tctaaaaaag gcggcacagt cacatacgaa
     8581 tttaccgttc ctactttaga cggatcacct gcagcgaaac ggaaagtgac atttgtaaaa
     8641 gaaaataaaa aatggaaagt caatcaattt gacgcagtta tataagaaaa aacgccctgc
     8701 ataagggact gaccccataa gatgagacaa ataaaaacac cttcaagttt gaatacggat
     8761 gattgttatc cgaaattaga cttggaggtg ttttttctat ggggacaaga gtgagttatc
     8821 cggttgaagt gaaa
//
