LOCUS       NZ_JALAPZ010000012     13070 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain CK3O2B-55B contig_0000012, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAPZ010000012
VERSION     NZ_JALAPZ010000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 336027)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 336027)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203.06051644694466x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 01:56:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,214
            Genes (coding)                    :: 4,095
            CDSs (with protein)               :: 4,095
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 71 of 119
            Pseudo Genes (incomplete)         :: 75 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 39 of 119
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:03:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 322957
            Orig. end                         :: 336027
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAPZ010000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13070
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="2"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..13070
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13070
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[10000:10747](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10747)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            complement(426..1358)
                     /gene="hprK"
                     /locus_tag="MOC81_RS17765"
                     /old_locus_tag="MOC81_17730"
     CDS             complement(426..1358)
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="MOC81_RS17765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17730"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_003235406.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGVNRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEDKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQDE"
     misc_feature    1470..1485
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            1540..2730
                     /gene="nagA"
                     /locus_tag="MOC81_RS17770"
                     /old_locus_tag="MOC81_17735"
     CDS             1540..2730
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="MOC81_RS17770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17735"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_268282383.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.3e-42, bitscore:
                     139.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKSLLIKGIAIVTENEVIKNGYVGINGGKISTVSTERPKESYSK
                     EIQAPADSVLLPGMIDIHIHGGYGADTMDASFSALDTMSSRLPEEGTTSFLATTITQEH
                     GHISQALVNARDWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIHYLKDESIIASMGHTDADSALLSEAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGKTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTEGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     gene            2727..3455
                     /gene="nagB"
                     /locus_tag="MOC81_RS17775"
                     /old_locus_tag="MOC81_17740"
     CDS             2727..3455
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="MOC81_RS17775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17740"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_268275536.1"
                     /transl_table=11
                     /translation="MKVVECQTYEELSQKAARIAADTIKEKPDAVLGLATGGTPEGTYR
                     ELIRLHQTENLSFKNVTTVNLDEYAGLSSDDPNSYHYYMNSRFFQHIDSQPNRHFIPNG
                     NADDLEAECRRYEQLVESIGGTDIQLLGIGRNGHIGFNEPGTPFKSRTHVVTLNEQTRQ
                     ANARYFSSIDSVPKKALTMGIQTILSSKRILLLISGKSKAEAARKLLEGDVSEDFPASA
                     LHLHPDVTILIDREAAALRP"
     gene            3473..4204
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="MOC81_RS17780"
                     /old_locus_tag="MOC81_17745"
     CDS             3473..4204
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="MOC81_RS17780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17745"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_268275947.1"
                     /transl_table=11
                     /translation="MNIDKQSPIPMYYQIMEQLKAQIKSGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGFLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIESTEELAALLGCGHPSPIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYEHIEKYNSIPISRAKQELEPSAATSKEASILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(4223..8092)
                     /locus_tag="MOC81_RS17785"
                     /old_locus_tag="MOC81_17750"
     CDS             complement(4223..8092)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="MOC81_RS17785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17750"
                     /product="metallophosphoesterase"
                     /protein_id="WP_268327923.1"
                     /transl_table=11
                     /translation="MRKYTVIASLFLSFLSVLSGGHHESKAFPVIKQEPFQTPHYIPLL
                     ENPPQIDVKAEMKSNLMIEAQVRDNEYQAFSAFLFYKQSNELGYKMVPMDPTPGAIHKF
                     LAQIPKRLIWNSELEYYIVISNGKQRVESETKKLKLEGYQADLAHIPELLITELAVDTK
                     NTGRADGYEFIEIYNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERITIPSGETFVV
                     WLKPVGHPELTAEDFNRYYQTQLTEGKNLAVLEETEGMANTRPRSVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLDGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILAEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKSHGLRMNIENRDTLSGTQ
                     FLKATTDGRTNSIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPELLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLKWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEAEASLDGESI
                     TLPYHTSSSELLPGKHSLKITATDLAGNKTVIERTFKTEREHPDQPEIIDSEADTHRAK
                     LSVRVKDPTDDPMDVAFYRGFQYTARNNVKIFKHASLTEPPKSFVPENETPFTNKELER
                     VSAVDGKTVSTEKKELFPYHRFEVTVDPSVDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDHKPFTLKASVIASDFVRKSKMNVIVQDEIPPAKDMYTFAWMSDTQY
                     YAESYPHIFDKQTAWIKSNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSHRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDEDIAWMNQVLKKHPDRMAVLAFHEYLLVSGNRSPIGEKIFKKVVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPHLQDKNYYDTDTYGNKDEFSLSLDLKPRVKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDDLEPHSVYYWYTIVEDSFNGKTKSPIWKFKTKKETYRPSPDQFDFR
                     HVSNP"
     gene            8257..8730
                     /locus_tag="MOC81_RS17790"
                     /old_locus_tag="MOC81_17755"
     CDS             8257..8730
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="MOC81_RS17790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17755"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_268308952.1"
                     /transl_table=11
                     /translation="MDNSIHDELFQAIQQFALKRDKRVWQKVQIPSRGMSSAHLEHLKK
                     DWTLTQLHIVSCIHTSQNVNNSFLASRLHISKAAVSKAVQALLKHNIITVTKKPGNKKE
                     IFYTLTESGRELAALHEQLHEKTKEQYKQLFNEFSIDDLKTVTAFFNLWVKHM"
     gene            complement(8766..9986)
                     /gene="cypX"
                     /locus_tag="MOC81_RS17795"
                     /old_locus_tag="MOC81_17760"
     CDS             complement(8766..9986)
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235412.1"
                     /locus_tag="MOC81_RS17795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17760"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_268308950.1"
                     /sec_met_domain="p450 (E-value: 5.4e-38, bitscore: 125.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQSIKLFSVLSDQFQENPYAYFSRLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFVGDALDHL
                     SPFIKQNAENLLDPYLEKGRIDLVNDFGKTFAVCVTMDMLGLDKRDHEKIAEWHSGVAD
                     FITSIFQTPEARAHSLWCSEQLSDYLMPVIEERRVNPGSDLISILCTSEYEGMAMSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEHPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKTEIEIVANIVLDKMRNIRLEEGFRYAESGLYTRGPVSLH
                     IAFDRAQ"
     gene            complement(10001..10747)
                     /gene="pchC"
                     /locus_tag="MOC81_RS17800"
                     /old_locus_tag="MOC81_17765"
     CDS             complement(10001..10747)
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="MOC81_RS17800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17765"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_268292529.1"
                     /sec_met_domain="CDPS (E-value: 5e-83, bitscore: 272.4,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEMVTERKPVDFTAESLTQNCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWATAEFKNVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     AAHGGDSKAIHTFSDFAENNAYQLLRQEVEHAFFEQPHFRHACLDMSREAMIGRARGIS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVDETLLAYHRPWKLGEKISNHEFSIR
                     MRPNQGYLIVQETAQMLSETRITSEG"
     gene            11177..11686
                     /gene="pchR"
                     /locus_tag="MOC81_RS17805"
                     /old_locus_tag="MOC81_17770"
     CDS             11177..11686
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="MOC81_RS17805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17770"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_003235414.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVIDIPK
                     NMTSIHVIDCIGKHEPINNAGIAKKMNLSKANVTKISTKLITEEFINSYQLTDNKKEVY
                     FKLTRKGKRIFDLHEKLHKKKELAFYQFLDSFSQDEQNAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     gene            11709..12920
                     /locus_tag="MOC81_RS17810"
                     /old_locus_tag="MOC81_17775"
     CDS             11709..12920
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="MOC81_RS17810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17775"
                     /product="MFS transporter"
                     /protein_id="WP_268292528.1"
                     /transl_table=11
                     /translation="MAQMKSKAVLILYIVCFSAFFASLSQNIYSPIIPIIKESFHVSAA
                     MVNLSVSVFMIVTAIMQIILGTIIDFKGARFVLIAGILATTAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGSERGSAMGTYQMLLSAAPAVAPVLGGFIGGAAGY
                     EGIFWMLTAISVVMLVANSMIFPKDSPAGSKQQTKGNVFTHYKSIFTNRTGNVILALSF
                     VLFFIYFAVIVYLPILLTEHYHIDVSIAGLLYLPLALSTIAGTFLFKRIQAKISLNTLF
                     IGSNAVAACSIMLFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GIFNFIRYTGMAAGPMISAYLLTMMPSAMSFGLLGLVFAALSFCLLPQMYPPQKRVKQK
                     KHHM"
     CDS             complement(12979..>13066)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611387.1"
                     /locus_tag="MOC81_RS17815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOC81_17780"
                     /product="IS3 family transposase"
                     /protein_id="WP_165406572.1"
                     /transl_table=11
                     /translation="IEYIHYYNNIRIQTKLNNQSPINYRQLAV"
     gene            complement(12979..>13070)
                     /locus_tag="MOC81_RS17815"
                     /old_locus_tag="MOC81_17780"
ORIGIN
        1 agcggccaat cgcctggccg agcaaaatgc ttggtgcggc aatatccgca agcttccaga
       61 acgaaaggtt tttcactctt gaaaatacat aacctgttaa aatggcgccg attaacccgc
      121 cgtgaatggc aatgccgccc ttccaaatct taatgatttc tcccggatgt gccgcataat
      181 aatcccattc aaatgcaacg taataaattc gtgcgcaaat aatcgcaatc ggaatggcaa
      241 acaggacaag atcgataaac gtgtctttct gcagcccccg tttctcactt tccctcatcg
      301 caatccaaag ccctagcaaa gcgccgagac cgataatgat tccgtaccaa tggacggcta
      361 acggccccag ctgaaaagct atcggattga gtggttctat cgcttcattc atttaacgcc
      421 aactcctatt cgtcttgttc gccgtcttca atgacgtccg ccagcttgtt tgtaaattgc
      481 tcagcagcgt tcagacccat ccgcttcaat ctgaagttca tcgctgccac ctcgatgatg
      541 actgcgagat ttcggcccgg acggacggga atcgtcagtt ttgtaatttc tgtatcaatg
      601 attttcatgg tttcttcctc aagtccgaga cggtcgtatt gcttgccctg ctcccagagt
      661 tcaagattca tgacaatcgt aattcgttta ttgcttctga ccgcgccggc gccaaacagc
      721 gtcatcacgt tgataatgcc aagccccctg atttcaagga gatgctcaat taattcaggc
      781 gcgtttccga caagggtatc ctgatcctcc tgccgaattt caacgcaatc atcggcaacg
      841 aggcggtgcc cccttttcac aagctccagt gcggtttcac ttttcccgac gccgcttttt
      901 cccgtaatta gcacgccgac accgtaaata tcgacaagaa cgccatgaat cgctgtagta
      961 ggcgccagac ggctctcaag aaaattggtt aaacggcttg acagccttgt cgttttcagc
     1021 ggcgatctga ggacaggcac gccgtttttc tcggaagcgt caatcagctc ctgcgggata
     1081 ggcatatctc tggaaagaat aatagctggt gttacatcag tgcacagaga atccattcgc
     1141 tgctttttat cctcttccgg cagctgttca aagaaagaaa gctctgtttt tccgagaagc
     1201 tgcacgcgct ctctcggata atatgtaaaa tatccggcaa tttcaatgcc tggtcttgat
     1261 aagtcactca ttgtaatcgg acggttaacc ccttcttctc cgctgattaa ttccaaattg
     1321 aactgttcca ttacgtcttt tgtgcgaacc tttgccacga tatgttcctc ctgttccggg
     1381 ctgccccgag cttgctcaca atactttcat tttatcactt tcgggcttga acctaaaaca
     1441 gattttataa aagacgggaa aacacctcag ctggtctaga tcactagtct gaaaaggggt
     1501 aaaataaatg tattcatatt ccagaaaggc ggatcaccta tggcaaaaag tcttcttatc
     1561 aaaggcatcg cgatcgtgac agaaaatgaa gtaatcaaaa atggctatgt cggaatcaat
     1621 ggcggaaaaa tcagcacagt ttcaacagaa cggcctaaag agtcttactc aaaagagatt
     1681 caagcaccgg cagattccgt tttgcttccc gggatgattg acattcatat tcacggcggc
     1741 tatggcgcag atacgatgga tgcaagcttt tccgctctcg acaccatgtc ctcaagactt
     1801 ccagaagaag gcacgacgag ctttttagca acaaccatta cccaggaaca tgggcacatt
     1861 tcccaagctt tggtgaatgc aagagattgg aaagcagctg aagaatcctc tttattaggc
     1921 gcggaactgc tcggcattca tttggagggg ccctttgttt cacctaaaag agccggagca
     1981 cagccaaagg aatggatcag gccttcggat gttgcgcttt tcaaaaaatg gcagcaggag
     2041 gcgggcggac tgatcaaaat cgtcacactt gcgccggagg aggatcagca ttttgaactg
     2101 atccactatt taaaagacga atcgattatc gcgtcgatgg ggcacacaga tgccgactcc
     2161 gcattactgt cggaggccgc gaaagcaggc gcgagccata tgacccatct ttacaacgcg
     2221 atgagcccgt ttcatcaccg agagcccgga gtgatcggaa cggcgctagc ccatgatggg
     2281 tttgtgacag agcttatcgc tgacggcatc cactcccatc ctttagcggc aaagcttgct
     2341 tttttggcaa aaggcagcag caagctcatt ttaattaccg attccatgag ggcaaaaggc
     2401 ctgaaagatg gtgtatacga gttcggcggc caaagtgtga cggtaagagg aaagacagct
     2461 cttctttccg acggaacact tgccggttcg attcttaaaa tgaacgaagg cgcacggcat
     2521 atgcgtgagt ttacaaattg ctcttggacg gatatagcta atataacttc tgcaaacgcg
     2581 gctaaacagc tgggcatctt tgaccgaaaa ggcagtgtga cagagggaaa agatgcggat
     2641 cttgtgattg tcagcagtga ttgcgaggtg attctcacca tctgccgcgg aaacattgca
     2701 tttatatcca aggaggctga ccagatatga aagtagtgga atgccaaaca tatgaagagc
     2761 taagccaaaa agcagccaga atagcagcag acacaatcaa ggaaaaacct gacgccgttc
     2821 tcggtttagc aacaggcggc acaccggaag gcacgtaccg cgagctgatc cgcctgcacc
     2881 aaactgagaa tctttcattt aaaaacgtca ccacggttaa tttggatgaa tacgccggac
     2941 tttcaagcga tgatccgaac agctatcact actatatgaa ttctcgtttt tttcagcata
     3001 tcgacagcca gccaaaccgg cattttattc ccaatggaaa tgcagacgac ctggaagccg
     3061 aatgcaggcg gtacgaacaa ctagtcgaat ccattggcgg cactgacatt cagcttctcg
     3121 gcatcggccg aaacggacat atcggcttca acgaaccggg aacgcctttc aagtcgcgaa
     3181 cacacgttgt gacattaaat gaacaaaccc gccaggcgaa tgccagatat ttttcttcaa
     3241 tagacagtgt gccgaaaaaa gcactcacaa tgggaataca gacgattctc tcaagcaagc
     3301 gcattctgct gcttatttcc ggcaaaagca aagcggaagc tgcgagaaag ctgttagaag
     3361 gagacgtaag cgaagatttc ccagcatcgg ctttgcacct gcaccccgat gttacgattt
     3421 tgattgatcg tgaagccgcg gcattaagac cttgaaagga acatgctgac ttatgaatat
     3481 cgataaacaa tcgcctattc cgatgtatta tcagattatg gagcaattaa aagcccaaat
     3541 taagagcgga gagctgcagc cggatatgcc tcttccctcc gagcgtgaat atgccgaaca
     3601 attcggaatc agccggatga cggttcgcca ggcgctttct aatttagtca atgaaggctt
     3661 tctttatcgc ctgaaaggac ggggcacctt tgtcagcaag ccaaaaatgg aacaagcact
     3721 ccaagggctt acaagcttta ccgaggatat gaaaagccgc gggatgacac cgggcagcag
     3781 gctcattgac tatcagctta ttgagtcaac tgaggagctc gcagctttat taggctgcgg
     3841 gcatccctct cctatccata aaatcactcg ggtgcggctg gcaaatgata ttccgatggc
     3901 aattgaatcc tcgcatattc cgttcgagct tgcgggtgaa ttaaacgaat cgcattttca
     3961 gtcgtcgatt tacgagcata ttgaaaagta caacagcatc ccgatttccc gtgcaaaaca
     4021 ggagcttgaa ccaagcgctg caacatcgaa agaagcaagc atccttggca ttcaaaaagg
     4081 ggcgcctgtc ctgttaatca aacgaacaac gtatttacag aacggaactg cttttgaaca
     4141 tgcaaaatca gtatacagag gcgaccgtta tacatttgtc cactatatgg accggctttc
     4201 ataaaaaaat ctccaacccc ttttaaggat tggagacatg gcgaaaatca aactggtctg
     4261 gcgacggacg atatgtctct tttttcgtct tgaacttcca gatcggtgat ttcgttttgc
     4321 cgttaaagct gtcttccaca atggtgtacc aataataaac agagtgcggt tcaagatcat
     4381 cccagcggaa ttcagcggta tccccgctct tcacttgctc ccgttttccg agctcttcat
     4441 tggtatatac attacattca aaataatcgg tttccacctt tttgaccctc ggcttcaaat
     4501 caagcgaaag cgaaaactca tccttgttgc cgtaggtatc cgtatcatag tagtttttat
     4561 cttgtaaatg cggagagtac gtcgacacat gcaccatatc attcgcctgg tcaaactgca
     4621 gcaaacgcaa atacccttgc ccgccttctg gcccgccctg ataatcggcc agcatttgat
     4681 gcacgaggcg atccggtttt ccatcaccat catcatccag ctcatcagtt tttctcatcg
     4741 cactatgata atgcccgctt aacaccatca ccacattcgg attcggcttc acgacttttt
     4801 tgaatatctt ctctccgatc gggcttctgt ttccgctgac cagcaaatac tcatgaaaag
     4861 ctaaaacagc catccggtca ggatgcttct tcagcacttg attcatccac gcgatgtctt
     4921 catccgtaat gccccatccc atgtacagca tgatataatc attgccgttg ctcgaaatta
     4981 agtcataatg tccgcggtta tttttataag aaccgccata atgcaatttt ttatcaaaac
     5041 gatgactgcc aaagtatttg ccaaacgccc tgtaggagcc gtccttatgc ccgacatcgt
     5101 ggttacctgc cagcacgccg taaggaatcc cgcttttatc cagaacagac attgaccgat
     5161 cagcgttttt ccactgtctg atatcggcag aatcatctac aatatcaccc gtgtgaaaca
     5221 catatttgat attgagctgc ttttgattgc ttttgatcca tgcggtctgt ttatcaaaaa
     5281 tatgcggata gctttccgcg taatactgtg tatcggacat ccaggcaaat gtgtacatat
     5341 ccttcgcagg aggaatttca tcctggacaa tcacattcat cttcgacttc cgcacaaaat
     5401 cggaggcgat tactgacgcc ttcagcgtaa acggtttgtg atctttggca acgaagctgt
     5461 caaccggctg ccactcgttt gtccggtaat tccaagcata catcgtcacc tttcttcccg
     5521 gcaaagaact tcccttccag accgcttccg ccaagtcatt ctcatcaact gacggatcaa
     5581 ccgtcacttc aaagcgatga taagggaaaa gctccttctt ttctgtcgat actgtttttc
     5641 cgtcaacagc agatacccgc tccaattctt tattcgtaaa gggggtctca ttttccggca
     5701 caaagctttt cggcggttca gtcagtgacg cgtgcttgaa tattttcaca ttgtttcgtg
     5761 ctgtatattg aaatcctcta taaaaagcga catccatggg gtcatccgtc ggatccttca
     5821 cccgcacaga aagcttagcc ctatgcgtat cggcttcgct gtctatgatc tccggctgat
     5881 ccggatgctc acgttctgtt ttaaacgtcc gctcaattac tgttttattg cccgcgaggt
     5941 ccgttgctgt tatttttaga gaatgttttc ccggcaaaag ttcagaagat gacgtatggt
     6001 agggaagtgt aatgctctca ccatctaaag acgcctccgc ttcctcaatt ttgctccatt
     6061 tatcatacat atccgcctgc aggatgaggt ttcctttgta cgtttgtccc tcggtgatat
     6121 tcggctcgat atgcggcccc gaattatcta cacgcacaac caagcttaca ttttccttgc
     6181 cgtcgcttgc ttgaatatgg tgagcgcctt cagaaagctt tgatgtatcc cacttcagca
     6241 actgtgacgt aaacagtccg tcaggaagat tgaattgaaa ttgccagctt ttattcgagc
     6301 gctgattatc tccaataaag agttccttct caggagaaac cctctgatcc ctgatcaccg
     6361 tcccatccga taacactaat cgggcgtttt tgactaagaa atcatcacgg ttttcagccg
     6421 tatcaaatgg agacaccttt gaacctgatc gtatcgtaat acgctgcagc tgcttccctt
     6481 tacggagcag caactctggt aagggaacag aataggtgcg gtatttattt gttgtatcat
     6541 caaatacctt taacactttg ccttccatcg ttaccgcgtt tttaaagtaa agattcgttt
     6601 ttctcgtatc gaacgcaaaa tacacttctt tttccatagc gggctctgtc acttgttttt
     6661 ttccgtctat ccacagctta atgctgttag tgcgcccgtc tgttgtcgct ttgagaaact
     6721 gagtccccga tagcgtgtct ctgttttcaa tgttcatccg caaaccgtgc gatttgcttg
     6781 tttgtttaat atcaatcatt ttgaccggcg ttctcgcctc attgattccg tcccctgctg
     6841 ccacgtaata ctcaagctga tccttcccga tcagttcagg cgaatacaca atataatggt
     6901 agagattgtc gttgcggtct ttttcagcga gaatgcgctt aaacggtttg ttttcatctg
     6961 tccgatagta gaaggccgct gttttcacaa gactgcggtc ccgtatatca gcccgcagct
     7021 caatgctttc agcagggcga acaggcttgc ggtcagtcaa atcctcaatg accggctttt
     7081 ctttatcaag cttaattttt cgtttctgct caggaacttg cgcctgtaat accgttcccg
     7141 gggacggatt tttttctccg attgagatat tgagaagttc tgcggttccg tccagcggat
     7201 acttgtaaag cacagatgac agccggcgca gcgcatgttt ccgataatgc gcgaccgaaa
     7261 tatcttttcc tgtattcgtt gatatcacaa ctgaccgcgg cctagtattg gccatcccct
     7321 ctgtctcttc taatactgcg agatttttcc cttccgttaa ttgtgtctgg taatatcgat
     7381 tgaaatcctc cgccgtcaat tctggatgcc cgacaggctt cagccaaacc acaaaagttt
     7441 cgccggatgg aatggtgatc ctttcatccg gtctccaaat cagatcggag tcaggccctt
     7501 ctttcggata gcggtaacgg atatgatagt ctttaaaatc aatcgttctg tcagtcgtat
     7561 tataaatctc aataaattca taaccgtctg cacgtcccgt gttcttcgta tcaaccgcta
     7621 gctctgtgat cagaagctcc ggaatatggg cgagatcagc ctgatatcct tcaagcttca
     7681 gtttcttcgt ctccgactcg acccgctgct tcccattgga aatcacaatg taatactcaa
     7741 gctcgctgtt ccaaatcaat cgtttcggaa tttgcgccaa aaacttgtga atcgctccgg
     7801 gtgtcgggtc catcggcacc attttgtagc ctagctcatt actttgttta taaaaaagaa
     7861 aggcagaaaa tgcttgatac tcattgtcac gcacttgagc ctcaatcatc agattgcttt
     7921 tcatttcagc tttcacatcg atttgaggag gattttcaag caagggtatg taatgaggcg
     7981 tttggaatgg ttcctgcttt ataacaggga aagctttcga ttcatgatgg ccgccagaca
     8041 aaactgaaag aaaagacaag aacaaagaag cgataactgt gtatttcctc atcaaaacac
     8101 ctcccttttc acgtattttg cgtctgaaca gggaagctta tgagttaatt tttgtgcaaa
     8161 aagaacaaaa aagcgtatgc ctcttgaaaa ttccactatt acccagtaaa ctgaggatag
     8221 ttaaccagtt aacaattaaa tcagagaggc agattgatgg ataacagtat tcatgatgaa
     8281 ttatttcaag ctatacaaca attcgcatta aaaagggata aacgcgtctg gcaaaaggta
     8341 caaataccat cccgcggcat gtcctcagca catctcgaac acctcaaaaa agactggaca
     8401 ttgacccagc ttcacattgt ttcctgtata cataccagcc aaaatgtgaa caacagcttc
     8461 ttagcatccc gcttacacat ctcaaaagca gctgtttcaa aagcagttca agctttgttg
     8521 aaacacaata tcatcacagt gaccaaaaaa cccggcaaca aaaaagaaat tttttatacg
     8581 ttaactgaaa gcggcagaga actggcggca ttacatgaac agcttcatga aaaaacaaag
     8641 gagcaataca aacagctctt caatgaattt tcaattgatg atttaaaaac agtgactgcg
     8701 tttttcaact tgtgggtcaa acatatgtaa aaaaagcctc acccgatggg agagaccttt
     8761 caatcttatt gagccctgtc aaacgcaata tgaagtgaga cgggtccgcg tgtatacaga
     8821 ccggactcag cataacgaaa accttcctct aacctgatat tccgcatctt atccagcaca
     8881 atattggcta caatctcgat ttcggttttg gcaaaagctg ctcctacaca gttatggatg
     8941 ccagatccga aagcgagatg ccgggcggcg ccgctaaaag cgctcttgat gccgagatct
     9001 tcccgatgaa tattaaacac gtcaggatgt tcaaacgctt ccgggtcccg gttagctgct
     9061 ccgatcatac aaaaaacaat cgtatccttt ttgatttcca taccgccgac caccgtatct
     9121 tgggacagct gccgcggaat cagctgaacc ggcggtttat aacgcaacgt ctccgcaatg
     9181 gctctcggca ccaatgaacg gtcagccaaa acatcattca tctgctcggg attgttgagc
     9241 aaatgataaa tcatcagcgc cagcgtctta tctgccggtt ccgttgcggc taacagcaca
     9301 ttgagaatca gtgccaatat atccttgtcc gacatcgcca tgccttcata ttcagacgta
     9361 cacaggatgg aaattaaatc tgatcccgga ttgacgcgtc gttcctcgat aaccggcatc
     9421 aagtaatcgg aaagctgttc gctgcaccat aaagaatgcg cccgcgcttc aggagtctga
     9481 aagatactcg taataaaatc ggcaactccg ctgtgccact cagcgatttt ttcatgatct
     9541 cttttatcca gcccgagcat gtccatcgtg acacacaccg caaacgtctt tccaaaatca
     9601 ttgacgagat cgattctccc tttttctaga taaggatcta ataagttttc tgcattttgc
     9661 ttaatgaatg gagacagatg atccaatgcg tccccgacaa agcttctcac tacaattctt
     9721 ctttttgcgg agtgttcttt cccatgcatt tgggccagca cagggccgcg catgaccggt
     9781 tcagcacgct caacaagtga ttttgtcgta aaaatatccg gatgctgaag gatatagcgg
     9841 acatcgtgat agcggctgat aaaataactg tctatcgact cttcataatg aacaggatct
     9901 tcctcccgca gccgtgaaaa ataagcatat ggattttctt gaaattgatc agaaagcaca
     9961 ctaaacaact taattgattg attcatgttt actcccccta tcatccttca gatgtgatcc
    10021 gtgtttcaga aagcatctgc gccgtttcct gcacaatgag atacccttga ttaggccgca
    10081 tacgaataga aaattcatgg ttactgattt tctcaccgag cttccacgga cgatgataag
    10141 caaggagcgt ctcatccacc tcaagaatat ccggagctcc gataaaaaac ggcagctcag
    10201 caatgacata ttccacagct aaattcagca tatcctcact gacttcttcc atcatcaaac
    10261 tgatgccgcg tgcacgcccg atcatcgctt cacgagacat gtccaaacaa gcatgccgaa
    10321 aatgaggctg ctcaaaaaac gcatgttcaa cttcttgtct caacagctga taggcattgt
    10381 tttccgcgaa atcagaaaat gtgtgaatcg cctttgagtc cccgccatga gccgcaagag
    10441 ccctttctgc aaatcttctg tttcgtgata cctcttttct tacttttcgc tcagctttgc
    10501 ctctgggagt tccgagcgct tcaagaagat tagccgcctc atgccccgca agcaaaacgg
    10561 aaacgttttt aaattcagcc gttgcccatc caatcaatct gtaaatataa tcctcagaaa
    10621 acctgctatt aaatgggctg atccccacca aaacatgccg gcgccgttca aatatttctc
    10681 tgcagttttg tgtcagtgac tcagcagtaa aatctacagg ctttctttcc gtcaccattt
    10741 cggtcatctc attcacctct aaaaacttat cccgctttaa gtcatcaccc tcaaaaatga
    10801 gatagaataa ctggcacaac tgaacaagac gaatggcagg cagcacttaa agtttctaag
    10861 tgaattattt taagatttaa aacttctttt tcattattca tatcatgata aatgtttact
    10921 agtaaacatt agtcatttta tcccttatcg ttccacccgt caatatgaat agacctattt
    10981 ttttgaaata aatgtaaatg tcacattttt cacacacttt ttacgctcct gttcagcatg
    11041 acataacacc ggataaagtt taaactccag caattgacaa cgaaagtaag aaaagataaa
    11101 attaaatcgt tcaccggtaa acaattttat ttattgtttc atcgtaaact attattcctc
    11161 agaaagaagg tagcctatgt ctgatttgac aaagcagatg atatacgaca tatatgtgag
    11221 actgctgcac cttaatgaac aaaaagcgaa cacttcactt cagcaatttt ttaaggaggc
    11281 cgcagaagag gatgtgatcg atatccctaa aaacatgaca agcattcatg tcattgactg
    11341 catcggcaag catgagccca ttaacaatgc ggggatcgca aaaaaaatga acttatcaaa
    11401 agcgaatgta acgaaaatca gcacaaaatt gatcacggaa gaattcatta acagctatca
    11461 gctgacagat aacaaaaaag aagtgtattt caaattaacc cgtaaaggca aacggatttt
    11521 cgacttgcat gagaaactgc ataaaaaaaa ggagctggcc ttttaccaat tcctcgattc
    11581 gttttcacaa gatgagcaaa atgctgtatt gaaattttta gagcagctga cgtctacact
    11641 tgaagcagaa caaaccgatg ggactccgga caaacctgta aagtaaacca aaaggcggtg
    11701 tattctttat ggctcaaatg aaatcaaaag cggtattgat cttatacatt gtttgcttca
    11761 gcgcattttt tgcatcttta agccaaaaca tttattcacc tataatcccg atcattaaag
    11821 aatcgttcca tgtttctgca gcaatggtga atctgtcagt ctccgtcttt atgattgtga
    11881 cagcgattat gcaaattata ttagggacca ttatcgattt taaaggcgcg cggtttgtct
    11941 tgattgccgg aattctggca acgacagcag ccagcatcgg ctgtgcggtg accactgact
    12001 ttactttgtt tctgatattc agaatgatac aggctgccgg gtccgcagca ctgcctctca
    12061 ttgctgccac aacgatcgga cagcttttta caggcagtga acgcgggagt gcgatgggaa
    12121 cgtatcaaat gctcctttcc gccgcgccgg ctgtcgcccc tgtcctcggc ggattcatag
    12181 gcggagcagc cggatacgaa gggatcttct ggatgctcac ggccatctct gtcgttatgc
    12241 tggttgcaaa cagcatgatt tttcctaagg actctcccgc aggatctaag cagcaaacca
    12301 aaggcaatgt gttcactcat tacaaatcaa tatttacaaa tcgaacagga aacgtcattt
    12361 tggccttaag ttttgtcctc tttttcattt attttgcggt gatcgtctac ctcccgatat
    12421 tactgacgga gcattatcat attgatgtga gcatagcagg attgttatat ttgccactgg
    12481 cgctaagtac gatcgcaggt acgtttttgt ttaaaagaat acaggcaaaa atctcgctga
    12541 acacattgtt tatcggcagc aatgcggtcg cggcctgcag tatcatgttg ttcgccgtta
    12601 cacattccgt ttctctcgtt ctcatggctc ttacactggc actgtttggc atctcgatgg
    12661 gggtcattcc tcccttgtac tccacaatga tcaccaatga atttgaacac aacagaggga
    12721 gtgcaatcgg aattttcaac tttatcagat acacaggcat ggcggcaggt ccgatgatat
    12781 ctgcctatct gctcaccatg atgccgtctg ccatgtcctt tggcctctta ggccttgtat
    12841 ttgccgcatt gagcttttgc cttttgcctc aaatgtatcc gccgcagaag cgcgttaaac
    12901 aaaaaaaaca ccatatgtaa aaaaagctgc cttctgtggg actgaccccc gtttttgaga
    12961 caggggtcaa aacacctttt aaacagccaa ttgccgatag tttatcggtg attggttgtt
    13021 tagtttggtt tgaatacgaa tattgttata ataatgaatg tattctatga
//
