LOCUS       NZ_JALAMD010000028      7596 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Bacillus inaquosorum strain N18A14 contig_0000028, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAMD010000028
VERSION     NZ_JALAMD010000028.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 223.65929725610533x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 03:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,188
            CDSs (total)                      :: 4,096
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 4 (5S, 16S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 82 of 127
            Pseudo Genes (incomplete)         :: 72 of 127
            Pseudo Genes (internal stop)      :: 17 of 127
            Pseudo Genes (multiple problems)  :: 37 of 127
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:22:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7596
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAMD010000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     cand_cluster    1..7596
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7596
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2100](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2100)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        complement(<1..711)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20775_AMP-binding.1"
                     /evalue="8.10E-60"
                     /label="MOB22_RS20775_AMP-binding.1"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="700"
                     /protein_start="463"
                     /score="194.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FERQAAFTPERPAIRFSSGSLTYAELDMYASRLAARLAARGVTKE
                     SIVGVLSERSPEMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDSGSALLLAQPGCSA
                     PSFSGEMLEVDMASLASEKAENDVFTPADGCSLAYVIYTSGSTGQPKGVAVEHRQAVSF
                     LTGMQRQFPLQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAI
                     HQEKVTTAHFIPAML"
     aSModule        complement(<1..2091)
                     /domains="nrpspksdomains_MOB22_RS20775_Condensation_Starter
                     .1"
                     /domains="nrpspksdomains_MOB22_RS20775_AMP-binding.1"
                     /incomplete
                     /locus_tags="MOB22_RS20775"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     gene            complement(<1..2100)
                     /locus_tag="MOB22_RS20775"
                     /old_locus_tag="MOB22_20720"
     CDS             complement(<1..2100)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_Starter (3-300). E-value:
                     1.1e-90. Score: 295.9. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20775_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (463-700). E-value: 8.1e-60.
                     Score: 194.4. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20775_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOB22_RS20775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20720"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268430127.1"
                     /sec_met_domain="Condensation (E-value: 2e-76, bitscore:
                     245.5, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.2e-56, bitscore:
                     179.8, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQHTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVL
                     EGALNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQA
                     RIPFKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDP
                     LPDQPEPSYLSYIEKESQYLQSSRFAKDRLFWTQTFQNPPEYHSLAGQTSLQKQSTSAS
                     RDTITLSPHLGQKIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKA
                     EKEMLGMFVSSLPIRITVDPDADFLSFVRRVGREQLSVMRHQRFPYNLLVNELRKEQKD
                     LHNLIGISMQYQPLQWHNADGFDYETALYFSGYTANELSVQIQERTDTGTIQLNFDYQN
                     TLFSLEDIKRIQNHLLTLLENALQHPHSFIKELDMTNIREKQKLLYEFNKTEAVSPEAP
                     TLHGLFERQAAFTPERPAIRFSSGSLTYAELDMYASRLAARLAARGVTKESIVGVLSER
                     SPEMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDSGSALLLAQPGCSAPSFSGEMLE
                     VDMASLASEKAENDVFTPADGCSLAYVIYTSGSTGQPKGVAVEHRQAVSFLTGMQRQFP
                     LQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAIHQEKVTTAH
                     FIPAML"
     source          1..7596
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="N18A14"
                     /submitter_seqid="contig_0000028"
     region          1..7596
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     CDS_motif       complement(241..300)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20775_0007"
                     /evalue="2.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="620"
                     /protein_start="600"
                     /score="35.1"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGQPKGVAVEH"
     CDS_motif       complement(484..525)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20775_0006"
                     /evalue="4.10E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="539"
                     /protein_start="525"
                     /score="19.8"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPLD"
     CDS_motif       complement(1048..1161)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20775_0005"
                     /evalue="1.30E-09"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="351"
                     /protein_start="313"
                     /score="30.6"
                     /tool="antismash"
                     /translation="REQLSVMRHQRFPYNLLVNELRKEQKDLHNLIGISMQY"
     aSDomain        complement(1201..2091)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20775_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="1.10E-90"
                     /label="MOB22_RS20775_Condensation_Starter.1"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="300"
                     /protein_start="3"
                     /score="295.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="HTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVLEGA
                     LNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQARIP
                     FKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDPLPD
                     QPEPSYLSYIEKESQYLQSSRFAKDRLFWTQTFQNPPEYHSLAGQTSLQKQSTSASRDT
                     ITLSPHLGQKIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKAEKE
                     MLGMFVSSLPIRITVD"
     CDS_motif       complement(1213..1302)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20775_0004"
                     /evalue="8.70E-09"
                     /label="C5_starter_265-294"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="296"
                     /protein_start="266"
                     /score="27.8"
                     /tool="antismash"
                     /translation="DLAIGTYYGNRGSKAEKEMLGMFVSSLPIR"
     CDS_motif       complement(1636..1701)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20775_0003"
                     /evalue="6.90E-08"
                     /label="C3_starter_134-155"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="155"
                     /protein_start="133"
                     /score="25.3"
                     /tool="antismash"
                     /translation="FHHIIMDGISLNVMGNQIIDLY"
     CDS_motif       complement(1906..2022)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20775_0002"
                     /evalue="4.70E-10"
                     /label="C2_starter_024-063"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="65"
                     /protein_start="26"
                     /score="32.1"
                     /tool="antismash"
                     /translation="ICNLTACVKFRGNIDLDVLEGALNHSISRNDAIRFQLLE"
     CDS_motif       complement(2047..2085)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20775_0001"
                     /evalue="4.00E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="MOB22_RS20775"
                     /protein_end="18"
                     /protein_start="5"
                     /score="10.1"
                     /tool="antismash"
                     /translation="YSLTHAQRRVWFT"
     gene            complement(2471..3949)
                     /gene="dacB"
                     /locus_tag="MOB22_RS20780"
                     /old_locus_tag="MOB22_20725"
     CDS             complement(2471..3949)
                     /EC_number="3.4.16.4"
                     /GO_function="GO:0004175 - endopeptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241941.1"
                     /locus_tag="MOB22_RS20780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20725"
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="WP_268315585.1"
                     /transl_table=11
                     /translation="MKKSIKLYVAVLLLFVVASVPYMHQAAQAAEKQGALAGQIDKILA
                     NHPALEGAMAGITVRSAETGAVLYEHSGEIRMRPASSLKLLTAASSLSVLGEDYSFTTE
                     VRTDGKFKGKKLNGNLYLIGKGDPTLLPSDFEEMAEKLKQSGVKEIKGNLIGDDTWYDD
                     IRLSPDMPWSDEYTYYGAQISALTASPNEDYDAGTVIVEITPGKKEGEKPAVSVSPKTD
                     YVTIKNDAETTAAGQKKDLTIEREHGTNTITIEGSVPVDASKTKEWIAVWEPAGYALDL
                     FKQALKKQGITVKGHVKTGAAPGSSDVLISHRSMPLSKLFVPFMKLSNNGHAEVLVKEM
                     GKVKKEEGSWEKGLEVLNNALPEFGVDPKSLVLRDGSGVSHINAVSSDQISQLLYDIQD
                     EKWFSAYLNSLPVAGNSDRMIGGTLRNRMKDTPAQGKVRAKTGSLSTVSSLSGYAETKS
                     GKTLVFSILLNGLIDDEDGKDIEDQIAVILANQS"
     gene            complement(3984..4961)
                     /gene="galM"
                     /locus_tag="MOB22_RS20785"
                     /old_locus_tag="MOB22_20730"
     CDS             complement(3984..4961)
                     /codon_start=1
                     /gene="galM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389718.1"
                     /locus_tag="MOB22_RS20785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20730"
                     /product="aldose 1-epimerase"
                     /protein_id="WP_225516923.1"
                     /transl_table=11
                     /translation="MANDIEKITYLGTPAIKAGNEHIEMIVVPEWGSNVISLVDKTTQL
                     QLLREPETAESFHDTPTLYGIPILFPPNRISDGTFSFRGRTYHFDINEKDKHNHLHGFL
                     YQEKWNVVTMKQTDEEVIVETEIDLSELPHVYKQFPHHAVVRMTYTLKGNTLFKHATVM
                     NKGKEAFPWGIGYHTTFVFPEESSLFSLTADQQWELDERLLPTGKLMDVQNKEALHEGM
                     DLRSQQLDDVFLSSYQKRGGENQAVIYHQHAHISIIYKADEQFKHWVVYNADGKQGYLC
                     PEPYTWVTNAVNLNVSPSLTGVQVLEPGEETTAKSSITIELNHQ"
     gene            complement(5092..6483)
                     /locus_tag="MOB22_RS20790"
                     /old_locus_tag="MOB22_20735"
     CDS             complement(5092..6483)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389719.2"
                     /locus_tag="MOB22_RS20790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20735"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_268315586.1"
                     /transl_table=11
                     /translation="METSQEQLVTQKKSNPSVWRSMSLFLVPLLLSNVLQSVGQLVGMM
                     AVGRWLGVDAVAAVSSFFPLFFLLISFTIGIGSGSSILIGQAYGAKNEERLKAVVGTTL
                     TFTFLLGVVLAVIGSIFTLDILRLMGTPENVIHVSASYARILFYAMPFMFLYFAYTTFL
                     RGTGDSKTPFYTLIVSTVINIALLPVLILGMFGFPKLGIYGSAYATVISTIATFIVLMV
                     YLRKRNHPLQFDKTVRRYLKMDKELLALLLRLGIPSSINMILVSLSEIAVISFVNHYGS
                     NATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANEFGRLKQVIRVGIWLNYIIGG
                     VLIILIYVFSHQILGLFLTEQETLYIAHRLLMITLWSYLLFGNAQIISATMRASGTVLW
                     PTVISIFAIWGVEVPVAFVLSHYTNLEILGIWVGYPAAFAVSLLLIYGYYQFVWKKKQI
                     TRLIQ"
     gene            6770..7315
                     /gene="iseA"
                     /locus_tag="MOB22_RS20795"
                     /old_locus_tag="MOB22_20740"
     CDS             6770..7315
                     /codon_start=1
                     /gene="iseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389720.2"
                     /locus_tag="MOB22_RS20795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20740"
                     /product="DL-endopeptidase inhibitor IseA"
                     /protein_id="WP_003241937.1"
                     /transl_table=11
                     /translation="MKKCFLFLTTIALILSLSTNAFAKNTSGDLSQKQALQLALSAREH
                     FWNTMSGHNPKAKKAVCPSGTFEYQNLQYVYMCSDLGTKAKAVNYLTPIFTKTAIEKGF
                     KDYHFTVSKGKLAVPIGDGDNLLNWKKSTAKLISKKGGTVTYEFTVPTLDGSPAAKRKV
                     TFVKENKKWKVNQFDAVI"
     gene            7429..>7596
                     /locus_tag="MOB22_RS20800"
                     /old_locus_tag="MOB22_20745"
     CDS             7429..>7596
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611387.1"
                     /locus_tag="MOB22_RS20800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20745"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_268284434.1"
                     /transl_table=11
                     /translation="MGTRVSYPVEVKQKAVEMRLAGVPMKEIMQELNIKNKTQVQTWVR
                     WHKAGDTHRFE"
ORIGIN
        1 cagcatagcc ggaataaaat gagccgttgt cactttttcc tgatgaatgg cttttacaat
       61 caatgccggg tctttctccc agccgggcgg aagcagataa gccgaagcgc cggaaaggga
      121 ccaccaaaac agctgccaca cagacgcatc gaaggaaaaa gaggttttca ccataacaat
      181 gtcatcttcc tgaagcggaa actgacgctg catgccagtc agaaaggaaa cagcctggcg
      241 gtgctcaacc gctacccctt ttggctgccc ggtggagccc gatgtataga tgacataggc
      301 gagagaacag ccgtcagcag gagtaaacac atcgttttct gctttttcgc tcgcaagaga
      361 tgccatgtcc acttcaagca tctctccgga aaagctcggt gcggaacatc ccggctgtgc
      421 cagcaagagc gcggacccac tgtctttcag catgtagctc agccgctcct ttggatatgc
      481 aggatcaagc ggcaaatacg ccccgcctgc cttcaagaca gcaagtacag ctatcagcat
      541 ttcaggtgac cgttcagaca ggacaccgac gatgctttcc ttcgtgacgc cgcgtgccgc
      601 gaggcgcgcg gccagccggc tggcatacat gtcaagctca gcgtacgtca gcgaaccgct
      661 ggaaaagcgg atggctggcc gctccggtgt gaaagccgcc tgtcgttcaa acagtccgtg
      721 cagggtcggg gcttcaggcg aaacagcttc cgtcttatta aactcataca acaatttttg
      781 tttctctctt atattagtca tatctaattc ttttatgaaa ctgtgcggat gttgaagggc
      841 gttttccagt aacgtaagca gatggttctg tattcgctta atatcctcaa gagaaaacaa
      901 ggtattttga tagtcgaaat ttaattgaat agttcctgtg tctgtccgtt cctgtatttg
      961 aacagaaagc tcgttcgcgg tgtatccgct gaaataaagc gccgtttcat agtcaaaacc
     1021 gtctgcattg tgccattgga gcggttgata ctgcatagat ataccgataa ggttgtgtaa
     1081 atccttctgt tcttttctta attcatttac cagcaaatta taaggaaacc gttggtgacg
     1141 cataacggaa agctgttccc tgcctactct gcggacaaag gatagaaagt ccgcgtcagg
     1201 atcaaccgtt attctgatgg gaagagaact gacgaacatg ccgagcattt ctttttcagc
     1261 ctttgaccct ctgttgccat agtaggtgcc gatggcaagg tcttttttag aagtgatccg
     1321 gctgatgcat atgtaaaatg aagccataaa taaggaaatg atattgatct tttgttcttc
     1381 gcaaaagatt cgaatctttt gccctagatg aggtgaaagg gtgatggtat ctctggatgc
     1441 ggatgtgctc tgtttttgta aagacgtctg gccggctaac gagtggtatt ccggcggatt
     1501 ctgaaaggtt tgagtccaaa ataaacggtc ctttgcaaaa cgtgaagact gtaaatattg
     1561 actctctttt tcgatgtagc tcaagtaaga aggttcaggc tgatcgggta aaggatcttg
     1621 ctttttcatt ttttgataaa gatctataat ttgatttccc atcacgttta aggagatgcc
     1681 gtccattata atatgatgga atttagcaaa cagccaaacc tcatgattgt ggattctaag
     1741 gagataaaat tgataaagag gcgagttgaa tagtttaaat gggattctcg cttgttcttg
     1801 tatccattgc tctatttcta tcatttcaac atttgaaaaa tctattattc ccagtgaagt
     1861 atatttatat tcagtaaggt acaatcgcgg ctcaagctct tctccttcta gcagctgaaa
     1921 cctgatggcg tcgttgcggg agatggaatg attcagcgcg ccctctagaa catcaagatc
     1981 aatattgcct ctgaatttca cacaggccgt aagattgcaa atgctggtac ctggctccag
     2041 cagttcggta aaccacactc tcctttgggc atgggttaaa gaataagtat gttggctcaa
     2101 cggattccct ccagttctca taataagagg ataagaaaaa agtagaaaaa tgacaataaa
     2161 agaaattata tgactatata atccatatat gcactatatt aacggatatt ttttaaagta
     2221 caataaaaaa tattaaattt ttataacatt tttttgaata tagtattatt tctttagttt
     2281 tggtttcttt cttcatcaaa tttttgaaca taaagtgata aaagtatcat ttcgattcgg
     2341 gtcttacaca ctaatttttc aattgtgcta atttgctgtt gcgcgtaatc tttctatttg
     2401 ttggaagatg cgaccggaat attattgaag acataaaaaa acctgttttc cttggaaaac
     2461 aggtctgacg ctatgattga tttgccaaga tgacagcgat ttgatcctcg atatcttttc
     2521 catcctcgtc atcaatcaat ccgttcaaaa gaatagaaaa aacaagggtt tttccactct
     2581 tggtttctgc gtacccggat aaagagctga ctgtgcttaa cgatccggtt tttgccctta
     2641 cctttccttg tgcaggagtg tctttcatcc ggttcctcag tgttccgcct atcatcctgt
     2701 cactattgcc cgcaaccggc agagaattta gataagctga gaaccatttc tcgtcttgaa
     2761 tgtcatataa cagctgtgag atttgatctg aggatacagc attaatatgg gagacacctg
     2821 atccgtctct taagacaagt gatttgggat caacaccaaa ttccggaagc gcattgttta
     2881 atacttcaag tcccttttcc cagcttcctt cttctttttt cactttcccc atctctttta
     2941 cgagcacttc ggcatgtccg ttattgctta atttcataaa cggcacaaat agttttgaca
     3001 gaggcattga gcgatgagag attaacacgt ctgaagaacc gggcgcagcg cctgtcttga
     3061 cgtgcccctt tacagtaatt ccttgttttt ttagtgcctg cttaaataaa tccaaagcgt
     3121 atccagccgg ctcccagaca gcaatccatt cctttgtctt gcttgcgtca accggtacgc
     3181 tcccttcaat tgtgatcgtg tttgttccat gctctctttc gatcgtgagg tctttctttt
     3241 ggccagccgc agtcgtttcg gcatcgttct taatcgttac ataatccgtc ttcggagata
     3301 cagaaacggc aggcttttcc ccctcttttt tacccggggt gatctctaca atcacagtgc
     3361 ctgcatcata atcttcattc ggagacgctg ttaaagcgga gatttgcgcg ccgtaatatg
     3421 tgtattcatc actccacggc atatcaggcg ataatcggat gtcatcatac cacgtatcat
     3481 caccgatcag attgcctttg atctctttca cgccggattg tttcaatttc tcagccattt
     3541 cttcaaaatc agatggtaaa agtgtcggat ctcctttccc tatcaaataa aggtttccat
     3601 tcagcttttt ccctttaaat ttgccgtccg tccggacctc ggttgtaaag gaatagtcct
     3661 cgccaagaac agaaagtgat gaggccgctg tcaaaagttt taaagaagag gcgggcctca
     3721 ttcggatctc ccctgaatgc tcatatagaa cagcgcctgt ttccgcagaa cgaacggtga
     3781 tgccggccat tgcgccttcg agtgcaggat gattagctaa aatcttatcg atctgcccgg
     3841 caagtgcacc ttgtttttca gccgcttgcg cagcctgatg catataaggt accgaagcaa
     3901 cgacgaaaag cagcaaaaca gcaacataaa gctttatgct ttttttcatt attttacccc
     3961 gcctctctgc gcctcatcat tggctattga tgattcagtt caatggtgat actgcttttg
     4021 gctgtcgttt cttctcccgg ctccagcact tgtacaccag tgagtgatgg agacacatta
     4081 agattcacag cgtttgtcac ccatgtgtaa ggttcagggc ataaatatcc ctgctttccg
     4141 tccgcgttgt atacaaccca atgtttaaat tgttcatctg ccttataaat aatgcttata
     4201 tgtgcatgct gatgataaat gactgcctga ttttctccgc cccttttctg ataagaagat
     4261 aaaaagacat catccagctg ctggctgcgt aaatccatcc cttcatgcaa ggcttcttta
     4321 ttctgtacat ccatcagttt cccggtcggc agcaggcgct catccaactc ccattgctga
     4381 tctgctgtca gggagaataa cgagctttct tctggaaata caaatgtagt atgatagccg
     4441 attccccaag gaaacgcttc ctttcctttg ttcatgaccg tcgcatgttt aaatagagta
     4501 ttccccttta gcgtgtatgt catccgaacg acggcatgat gcggaaattg cttatacaca
     4561 tggggaagct ctgaaaggtc aatttcagtt tcaacgatca cttcttcatc agtttgcttc
     4621 atggtcacta cgttccattt ttcctgataa agaaacccat gaagatggtt gtgtttatcc
     4681 ttttcattga tatcaaaatg atatgttctg ccccggaaac tgaatgtacc gtcacttatc
     4741 cggttaggcg gaaacaggat cggaatgcca tacaatgttg gtgtatcatg aaagctttct
     4801 gctgtttccg gttcacgcaa aagttgaagc tgtgtcgttt tgtccacaag agaaatgacg
     4861 ttgctccccc actcgggaac gacaatcatt tctatatgtt cgttgcctgc tttgatggca
     4921 ggtgttccta aatacgtaat cttctcaata tcgtttgcca tgtgtactca acctctcctt
     4981 gattcattgc ttgtataaga attcaatgta atgtgccata tttcctccat ttatgttttc
     5041 tctttatcac aaaaaacacc gttcaaatgt tgaacggtgt gattcgggat gctattggat
     5101 gagacgtgta atctgtttct ttttccaaac gaattgataa tacccataaa tcaacagcaa
     5161 gctgacggca aacgcagccg gatagccaac ccagatgccg agtatttcaa gatttgtgta
     5221 gtgggaaagt acgaacgcga caggcacctc tactccccag atggcaaaaa tactgataac
     5281 ggtcggccat aaaaccgttc cgcttgcccg catcgtcgca gaaataattt gggcatttcc
     5341 aaagagtaag taactccaca atgtaatcat caagagacgg tgcgcaatgt aaagcgtctc
     5401 ttgttcggtt aaaaataatc ctaaaatctg atgtgaaaaa acatagatca gaatgatcag
     5461 cacaccgcca atgatataat tcagccaaat cccgacacga attacctgtt ttaggcggcc
     5521 aaactcgttc gctccgatcg attgcgcggc gaatatagac accgcaatac caaggctgac
     5581 ggccggcatc tgcacatagc tggctacctg gttgaccacg ccgtatgcag ctgtcgcatt
     5641 agagccataa tggttgacga atgaaataac cgctatctct gataatgaga cgagaatcat
     5701 attgatgctt gagggaatcc cgaggcgcag caggagagcc agaagttctt tatccatttt
     5761 caaataacgg cgcactgttt tatcaaattg cagcggatgg tttcgttttc gcaaatacac
     5821 cattaacaca ataaaagtcg caatcgtgga aatgacggtt gcatatgcgg agccgtatat
     5881 gcccagtttc ggaaaaccga acatgccaag gatcaagaca ggcaaaagcg ctatgttaat
     5941 gaccgtgctc acaatcagcg tataaaaagg tgtcttagaa tccccagttc cgcgcaaaaa
     6001 tgttgtataa gcaaaatata aaaacataaa cggcatcgcg taaaataaaa tacgtgcata
     6061 gctggcgctg acatgaatta cattttcagg cgtgcccatt aaacgaagaa tatccaaggt
     6121 gaaaatactg ccgatgacgg ctaacacaac ccctaataaa aacgtaaacg tgagcgtcgt
     6181 cccgacaaca gcctttaacc gctcctcatt tttcgccccg taggcttgtc cgatcaggat
     6241 cgagcttcct gaaccgatcc caattgtaaa tgagattaaa agaaaaaata acgggaaaaa
     6301 ggatgataca gctgcaaccg catcaacacc aagccatctg cctacagcca tcatcccgac
     6361 taactggccg actgattgca gtacattgct tagcagaagc ggaactaaaa agagtgacat
     6421 agaccgccag acagatggat tgcttttctt ctgagttaca agttgttctt gtgatgtttc
     6481 catattgaaa gctcctttat ttgaattcgt ttgtaaacat gaccctattc ttttgacaaa
     6541 aacaggatat gtaacatgtt attattcatc actgtacata tgaaaccctt cccgattctt
     6601 ctaattttac acatcttttt agcttatgaa gaataaacta gcaagtaaca gccttttttc
     6661 ttgtgagtaa aagaataaaa aataatttat gcaaaagtat tgtaatctat ccgtaattat
     6721 tgtaacattt gtaacataag agaacgagat tttgaaggag tgaaccacaa tgaaaaaatg
     6781 ttttctattt ctaacaacga ttgcacttat tctgtcatta agcacaaatg catttgcgaa
     6841 aaatacttca ggtgatttat cacaaaaaca agcactgcag ctggcgttat cagcaagaga
     6901 gcatttttgg aacacaatga gcggccacaa tccaaaagcg aaaaaagcag tttgcccatc
     6961 aggcactttt gagtatcaaa atcttcaata cgtgtacatg tgcagtgacc taggcactaa
     7021 agcaaaagca gtgaattact taactcctat tttcacaaaa acagcaatcg aaaaaggctt
     7081 taaagactat cacttcactg tttcaaaagg aaaactagcc gtgccaatcg gcgacggtga
     7141 caacctttta aattggaaaa aatcaactgc taaactgatc tctaaaaaag gcggcacagt
     7201 cacatacgaa tttaccgttc ctactttaga cggatcacct gcagcgaaac ggaaagtgac
     7261 atttgtaaaa gaaaataaaa aatggaaagt caatcaattt gacgcagtta tataagaaaa
     7321 aaagccgtgc gcacgggact gaccccataa gatgagacaa ataaaaacac cttcaagttt
     7381 gaatacggat gattgttatc cgaaattaga cttggaggtg ttttttctat ggggacaaga
     7441 gtgagttatc cggttgaagt gaaacagaag gctgtagaaa tgagattggc aggcgtgcct
     7501 atgaaagaga tcatgcagga attgaatatc aagaataaga cgcaggttca gacatgggtt
     7561 aggtggcata aggctgggga tacacatcga ttcgaa
//
