LOCUS       NZ_JALAMD010000021     19218 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Bacillus inaquosorum strain N18A14 contig_0000021, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAMD010000021
VERSION     NZ_JALAMD010000021.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 19218)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19218)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 223.65929725610533x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 03:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,188
            CDSs (total)                      :: 4,096
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 4 (5S, 16S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 82 of 127
            Pseudo Genes (incomplete)         :: 72 of 127
            Pseudo Genes (internal stop)      :: 17 of 127
            Pseudo Genes (multiple problems)  :: 37 of 127
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:22:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 19218
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAMD010000021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..19218
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..19218
                     /SMILES="NC(CCC(=O)O)C(=O)NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..19218
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[10243:>18691](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10244..>18691
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            <1..489
                     /locus_tag="MOB22_RS20270"
                     /old_locus_tag="MOB22_20220"
     CDS             <1..489
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611387.1"
                     /locus_tag="MOB22_RS20270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20220"
                     /product="IS3 family transposase"
                     /protein_id="WP_268430111.1"
                     /transl_table=11
                     /translation="SDHPLEKKLVTDITYLPYGQKQLYLSSILDVYNGEVIAFTIGDKQ
                     DTDFVLNTLDQLPTLPQNCVLHSDQGSVYTSYEYQKAVKTKGITMSMSRKGTPADNASI
                     ESFHSSLKSETFYLNSIDRTTTAIVERTVIEYIHYYNNIRIQTKLNNQSPINYRQLAV"
     source          1..19218
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="N18A14"
                     /submitter_seqid="contig_0000021"
     gene            complement(564..1244)
                     /locus_tag="MOB22_RS20275"
                     /old_locus_tag="MOB22_20225"
     CDS             complement(564..1244)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388219.1"
                     /locus_tag="MOB22_RS20275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20225"
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_268430113.1"
                     /transl_table=11
                     /translation="MINSIQWEYIFNTKLAIESFPYVIRGIGYTLLISFVSMFAGTVIG
                     LFISLARMSQLALLRWPAKLYISFMRGVPILVILFILYFGFPYIGIEFSAVTAALIGFS
                     LNSAAYIAEINRSAISSVEKGQWEAASSLGLSYWQSMRGIILPQSIRIALPPLANVLLD
                     LIKASSLAAMITVPELLQHAKIIGGREFDYMTMYILTALIYWAICSIAAAFQNILEKKY
                     AHYV"
     gene            complement(1254..2117)
                     /locus_tag="MOB22_RS20280"
                     /old_locus_tag="MOB22_20230"
     CDS             complement(1254..2117)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388220.1"
                     /locus_tag="MOB22_RS20280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20230"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_232918929.1"
                     /transl_table=11
                     /translation="MKSFMQSKAVIFSFTMAFFLILTACSGKNEAGSKDTGWEQIKDKG
                     KIVVATSGTLYPTSYHDTDSGSDKLTGYEVEVVREAAKRLGLKVEFKEMGIDGMLTAVN
                     SGQVDAAANDIDVTKDREKKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKKAAGAA
                     TTVYMDVARKYGAKEVIYDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPDLNITI
                     HPDIKYMPNKQALVMKKSNAALQKKMNETLKEMSKDGTLTKLSEQFFNKADVSKKLDVD
                     VQDVDL"
     gene            2500..2955
                     /locus_tag="MOB22_RS20290"
                     /old_locus_tag="MOB22_20240"
     CDS             2500..2955
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388221.1"
                     /locus_tag="MOB22_RS20290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20240"
                     /product="RDD family protein"
                     /protein_id="WP_268283663.1"
                     /transl_table=11
                     /translation="MNIYKPAGFWIRLGASLLDYIIVSVPLLLIYWLITGKDPNDSMFI
                     SLIVLLYSILLPVFWNGYLIGKRICGIRIVKKDGSQVSLLTMFLRVIVAGLVYGITFGL
                     GLIASLILIGVREDKRTLHDLIAGTYVTYASPGEEELENDEQIRKSE"
     gene            3038..3370
                     /locus_tag="MOB22_RS20295"
                     /old_locus_tag="MOB22_20245"
     CDS             3038..3370
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388222.1"
                     /locus_tag="MOB22_RS20295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20245"
                     /product="YckD family protein"
                     /protein_id="WP_268282919.1"
                     /transl_table=11
                     /translation="MKQMKINIIIMFIAAAVISLTGTAEAAEKQQQPPANVSLTDQQKK
                     EIEQLEADILKKRKDVISKYVQYGVLPKERGEHIKNHMDKHFEMMKQNGFVPKPHPHAH
                     KFEKRH"
     misc_feature    3046..3061
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 26.41"
                     /tool="antismash"
     misc_feature    complement(3048..3062)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.53"
                     /tool="antismash"
     misc_feature    complement(3401..3417)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.51"
                     /tool="antismash"
     misc_feature    complement(3474..3494)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.59"
                     /tool="antismash"
     gene            3522..4955
                     /gene="bglC"
                     /locus_tag="MOB22_RS20300"
                     /old_locus_tag="MOB22_20250"
     CDS             3522..4955
                     /EC_number="3.2.1.86"
                     /codon_start=1
                     /gene="bglC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388223.1"
                     /locus_tag="MOB22_RS20300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20250"
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="WP_268283664.1"
                     /transl_table=11
                     /translation="MIHQHPESFPKHFLWGSASAAYQIEGAWNEDGKGPSVWDVFTKIP
                     GKTFKGTNGDIAVDHYHRYKEDVALMAEMGLKAYRFSVSWPRIFPKGKGEVNEAGLAFY
                     DNLIDELLSHNIEPVLTLYHWDLPQALMDEYGGFESRNIIEDFNNYCITLYKRFGDRVK
                     YWVTLNEQNYNFNHGFITAMHPPGVKDRKRFYEANHIAFLANAKAIESFREYVPEGKIG
                     PSFAYSPAYPLSSHPEDILAFENAEEFMNNWWLDMYCWGTYPQIPFRYLEKQGWAPTIK
                     AGDMELLAKGKPDFVGVNYYQTITYERNPLDGVSEGKMNTTGQKGTNQETGIPGVFKTK
                     KNPHLTTSNWDWTIDPIGLRIGLRRITSRYQLPVFITENGLGEFDKVEDGTVQDDYRID
                     YLRSHLEQCRQAISDGVDLIGYCSWSFTDLLSWLNGYQKRYGFVYVNRDEENTHDLKRL
                     KKKSFYWYQDVIKTNGESL"
     gene            complement(4992..5390)
                     /gene="nin"
                     /locus_tag="MOB22_RS20305"
                     /old_locus_tag="MOB22_20255"
     CDS             complement(4992..5390)
                     /codon_start=1
                     /gene="nin"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388224.1"
                     /locus_tag="MOB22_RS20305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20255"
                     /product="DNA-entry nuclease inhibitor"
                     /protein_id="WP_003241129.1"
                     /transl_table=11
                     /translation="MIKSWKPQELSISYHQFTVFQKDSAPPVMDWTDEAIEKGYAAADG
                     AISFEAQRNTKAFILLRLNSSETVNSYEKKVTVPFHVTENGIEIESIMSKRLSFDLPKG
                     DYQLTCWTVPAEKSDLHADTYIIDAVSV"
     gene            complement(5417..5866)
                     /gene="nucA"
                     /locus_tag="MOB22_RS20310"
                     /old_locus_tag="MOB22_20260"
     CDS             complement(5417..5866)
                     /EC_number="3.-.-.-"
                     /codon_start=1
                     /gene="nucA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388225.3"
                     /locus_tag="MOB22_RS20310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20260"
                     /product="DNA-entry nuclease"
                     /protein_id="WP_133488530.1"
                     /transl_table=11
                     /translation="MNITMDIIKTILLVIVVIAAAAVSLIKGDFFSADQKTSQTKQYDE
                     TIAFPSDRYPETAKHIKDAINEGHSDVCTIDRDGAEERREQSLKDVPPKTGYDRDEWPM
                     AMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ"
     misc_feature    complement(6022..6035)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 21.98"
                     /tool="antismash"
     gene            complement(6041..7765)
                     /gene="tlpC"
                     /locus_tag="MOB22_RS20315"
                     /old_locus_tag="MOB22_20265"
     CDS             complement(6041..7765)
                     /GO_function="GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="tlpC"
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019257440.1"
                     /locus_tag="MOB22_RS20315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20265"
                     /product="methyl-accepting chemotaxis protein TlpC"
                     /protein_id="WP_268430114.1"
                     /transl_table=11
                     /translation="MLIKQLKLKLGTKILCMVFVIILLFSVSVGTVMLKEITESMKQMA
                     TEKAKGDLALSSAYIDDVISGDWQVKNNKLYKGKIQINGNEDIVDLLGEKTGDTVTIFQ
                     GDTRVATNVMKGGERAVGTQASSEVIDAVLKNGKRFYGQADVAGSSYQTAYMPLKDQNG
                     DIIGMLYTGANQSILASLTKSLFTQFAIVLAIVIIVSVILVLLFTRRINKRLNALKCAF
                     ESAGNGDMTIEVSDKSGDELSELSVYYNKMRMKLNNTIQTVQQSALQLASASQQLSAGA
                     EETNQASEKITEAVQHIANGAQHQITRIENSESSLKQASADIRDISSNTAAIADKGQLA
                     QSKADIGQKEIADVQAQMDAIHHSIQKSGEIIQQLDGRSKQIEQILSVITQIADQTNLL
                     ALNAAIEAARAGEHGKGFAVVADEVRKLAEESQQSAGQISTLITEIQKDMNQSARSVEH
                     VKTEAAEGVTMIQRTKDAFKEIAAATGEISAKISDLSASVSNISASAHQINDSFAANTA
                     DIKESTENTRQAAALTEEQFAAMEEITAASETLSQLAEELTGIISQFKMIDQPENGR"
     misc_feature    complement(7722..7735)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.26"
                     /tool="antismash"
     misc_feature    7722..7736
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.01"
                     /tool="antismash"
     gene            complement(7877..8434)
                     /gene="hxlB"
                     /locus_tag="MOB22_RS20320"
                     /old_locus_tag="MOB22_20270"
     CDS             complement(7877..8434)
                     /EC_number="5.3.1.27"
                     /GO_function="GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="hxlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388227.1"
                     /locus_tag="MOB22_RS20320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20270"
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="WP_119913388.1"
                     /transl_table=11
                     /translation="MKTTEYVTEILNELHRSAAYISNEEADQLADHILSSHQIFTAGAG
                     RSGLMAKSFAMRLIHMGFNAHIAGEILTPPLREGDLVIIGSGSGETKSLIHTAAKAKSL
                     HGIVAALTINPESSIGKQADLIVKMPGSPKDRSNGSYKTIQPMGSLFEQTLLLFYDAVI
                     LKLMEKKGLDSETMFTHHANLE"
     gene            complement(8440..9072)
                     /gene="hxlA"
                     /locus_tag="MOB22_RS20325"
                     /old_locus_tag="MOB22_20275"
     CDS             complement(8440..9072)
                     /EC_number="4.1.2.43"
                     /GO_function="GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /gene="hxlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388228.1"
                     /locus_tag="MOB22_RS20325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20275"
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="WP_003241120.1"
                     /transl_table=11
                     /translation="MELQLALDLVNIPEAIELVKEVEQYIDVVEIGTPVVINEGLRAVK
                     EIKEAFPQLKVLADLKIMDAGGYEIMKASEAGADIITVLGATDDATIKGAVGEAKKQKK
                     KILVDMINVKDIESRAKEIDALGVDYICVHTGYDLQAEGKNSFEELTTIKNTVKNAKTA
                     IAGGIKLDTLPEVIKQNPDLVIVGGGITSAADKAETASKMKQLIAQG"
     gene            9304..9666
                     /gene="hxlR"
                     /locus_tag="MOB22_RS20330"
                     /old_locus_tag="MOB22_20280"
     CDS             9304..9666
                     /codon_start=1
                     /gene="hxlR"
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388229.2"
                     /locus_tag="MOB22_RS20330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20280"
                     /product="transcriptional activator HxlR"
                     /protein_id="WP_003241118.1"
                     /transl_table=11
                     /translation="MSRMDDKRFNCEKELTLAVIGGKWKMLILWHLGKEGTKRFNELKA
                     LIPDITQKILVNQLRELEQDMIVHREVYPVVPPKVEYSLTPQGESLMPILDAMYEWGKG
                     YMELIDIDKNVIKESL"
     misc_feature    9802..9816
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.42"
                     /tool="antismash"
     misc_feature    complement(9957..9972)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.04"
                     /tool="antismash"
     misc_feature    complement(9958..9967)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.04"
                     /tool="antismash"
     gene            10244..>18691
                     /gene="srfAA"
                     /locus_tag="MOB22_RS20335"
                     /old_locus_tag="MOB22_20285"
     CDS             10244..>18691
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_Starter (4-303). E-value:
                     1.8e-96. Score: 314.9. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20335_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (466-867). E-value:
                     9.9e-114. Score: 372.0. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20335_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (976-1044). E-value: 2.1e-24. Score:
                     77.5. Matches aSDomain: nrpspksdomains_MOB22_RS20335_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1058-1345). E-value:
                     3.7e-105. Score: 343.1. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20335_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1510-1908). E-value:
                     1.5e-110. Score: 361.6. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20335_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (2015-2084). E-value: 1.8e-29.
                     Score: 93.8. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20335_PCP.2"
                     /NRPS_PKS="Domain: Condensation_LCL (2098-2384). E-value:
                     4e-108. Score: 352.9. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20335_Condensation_LCL.2"
                     /NRPS_PKS="Domain: AMP-binding (2550-2815). E-value:
                     4.8e-65. Score: 211.6. Matches aSDomain:
                     nrpspksdomains_MOB22_RS20335_AMP-binding.3"
                     /codon_start=1
                     /gene="srfAA"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="MOB22_RS20335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20285"
                     /product="surfactin non-ribosomal peptide synthetase SrfAA"
                     /protein_id="WP_268430115.1"
                     /sec_met_domain="PP-binding (E-value: 8.8e-16, bitscore:
                     48.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.2e-122, bitscore:
                     398.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.5e-98, bitscore:
                     318.2, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEITFYPLTDAQKRIWYTEKFYPDTSISNLAGIGKLVSTEKIDWK
                     LVEQAIQAFIRRNDAMRLRLRLNENGEPVQYISEYRPVDIKHTDTTGDPNAIESISQWG
                     REETKKPLPLYDCDLFRFSLFTIKENEVWFYANVHHVISDGISMNILGNAIMHIYLELA
                     GGSETKEGISHSFIDHVLSEQDYAQSKRFEKDKAFWNKQFETVPELVSLKRNVSAGGSL
                     EAERFSKDVPEVLHQQILSFCEANKVSVLSVFQSVLAAYLYRISGQNDVVTGTFMGNRT
                     NAKEKQMLGMFVSTVPLRTNIDGGQAFLDFVKDRMKDLMKTLRHQKYPYNLLINDLRET
                     KSSLTKLFTVSLEYQVMQWQKEENLAFLTEPIFSGSGLNDVSIHVKDRWDTGKLTIDFD
                     YRTDLFSREEIKIVCERMITMLENALFHPDHTIDELTLISEAEKEKLLVRAGGESVSYR
                     KDMTIPELFQEQAEQLSDHPAVVFEDRTLSYRTLHEQSARIANVLKQKGVGPDSPVAVL
                     LERSERMITSIMGILKAGGAYVPIDPAFPAERIQYILEDCGADFILTESQIEAPAADAE
                     LIDFDQAIAEGAGESLNADVNARNLAYIIYTSGTTGRPKGVMIEHRQVHHLVVSLQQTI
                     YQSASQTLRMALLAPFHFDASVKQIFASLLLGQTLYIVPKKTVTNGSALAAYYRRHCIE
                     ATDGTPAHLQMLIAAGDFEGLKLKHLLIGGEGLSSVVADKLLKLFKEAGIAPRLTNVYG
                     PTETCVDASVHPVIPENAVQSAYVPIGKALGNNRLYILDQKGRLQPEGVAGELYIAGDG
                     VGRGYLHLPELTAEKFLQDPFVPGDRMYRTGDVVRWLPDGTIEYLGREDDQVKVRGYRI
                     ELGEIEAVIQQASDVAKAVVLARPDEQGNLEVSAYVVQKPGSEFAPASLRAHAARQLPD
                     YMVPAYFTEITEIPLTPSGKVDRRKLFALEVKAVSGTAYTAPRNETEKAIAAIWQDVLN
                     VEKAGIFDNFFETGGHSLKAMTFLTRIHKETGAEIPLQYLFEHPTIAALAEEADHRESR
                     AFAAIEPAEKQEHYPLSLAQQRTYIVSQFEDAGVGYNMPAAAILEGPLEIQKLERAFQG
                     LIRRHESLRTSFILENNTPRQNIHDSVDFKVELMERGGRSDEEIMTSFVRRFDLAKAPL
                     FRIGLMKLEENRHMLLFDMHHLISDGVSIGIILEELARLYKGEQLPELRLQYKDYAVWQ
                     SGQAAEGYQKDRAYWKEAFAGELPVLQLLSDYPRPPVQSFEGDRVSIKLDAGLKNRLNR
                     LAEQNGATLYMVMLSAYYTLLSKYTGQDDIIVGTPSAGRNHSDTEGIVGMFVNTLAIRS
                     EVKQDETFTDLITRVRTQVLDAFSHQDYPFEWLVEDLNIPRDVSRHPLFDTMFSLQNAT
                     EGIPAVGDLSLSVHETNFKIAKFDLTMQARETDEGIELDLDYSTKLFKQSTADRLLTHF
                     ARLLEDAAAEPEKQISEYKLLSEEETASQIQQFNPERTPYPKDKTIVQLFEEQAAKTPD
                     QPALQYEGESLTYRELNERANRLARGILSLGAGEGRTVAVLCERSMDMIVSILAVLKTG
                     SAYVPIDPEHPIQRMQHFFRDSGAKVLLTQKKLKALAEEAEFSGVIVLADEEESYHADV
                     QNLALPLDSAAMANLTYTSGTTGTPKGNIVTHANILRTVKETNYLSITEQDTILGLSNY
                     VFDAFMFDMFGSLLNGAKLVLIPKETVLDMARLSRVIERENISILMITTALFHLLVDLN
                     PACLSTLRKIMFGGERASVEHVRKALQIVGKGKLLHMYGPSESTVFATYHPVDELEEHT
                     LSVPIGKPVSNTEVYIMDRAGHVQPAGIAGELCVSGEGLVKGYYNRPELTEEKFVPHPF
                     ASGERMYKTGDLARWLPNGDIEFIGRIDHQVKIRGQRIELGEIEHQMQTHDRVQESVVL
                     AVDQGAGDKLLCAYFVGEGEISSQELREHAAKDLPAYMVPAVFIQMDELPLTGNGKIDW
                     RALPLPDANVSRGVSYVAPRNETEQKVADIWAHVLQAEQVGVYDQFFDIGGHSLAGMKM
                     LALVHQELGVELSLKDLFQSPTVEGLAQVIDSAEKGTIISISPAKKQDTYPVSSPQKRM
                     YVLQQLEGAETSYNMPAVLRLTGELDVERLNSVMKQLMKRHEAFRTTFEIKDGETVQRI
                     WEEAECEMAYFEAAEEETERIVSEFIKPFKIDRLPLFRMGLIKHSDTEHVLLFDMHHII
                     SDGASVGVLIEELSKLYDGETLEPLRIQYKDYAVWQQQFIQSELYKKQEEHWLKELEGE
                     LPVLTLPTDYSRPAVQTFEGDRIAFSLEAGKVDALRRLAKETDSTMYMVLLASYSAFLS
                     KLSGQDDIIVGSPVAGRSQADVSRVIGMFVNTLALRTYPKGEKTFADYLSEVKETALSA
                     FDAQDYPLEDLIGNVQVQRDTSRNPLFDAVFSMQNANIKDLTMKGIQLEPHPFERKTAK
                     FDITLTADETDGGLTFVLEYNTALFKPETIERWKQYWMQLLDAVTGNPNQPLASLSIVT
                     ETEKQTLLDAWKGKTLPVPTDKTVHQLFEETAQRHKDRPAVTFNGQSWTYGELNAKANR
                     LARILIDCGISADERVGVLTKPSLEMSAAVLGVLKAGAGFVPIDPDYPDQRIDYILQDS
                     GAKLLLKQEGISVPDSFSGDVILLDGDRTILSLPLDENDEENPETDTNAENLAYMIYTS
                     GTTGQPKGVMVEHHALVNLCFWHHDAFSMTEEDRSAKYAGFGFDASIWEMFPTWTIGAE
                     LHVIDEAIRLDIVRLNDYFETHGVTITFLPTQLAEQFMELENTSLRVLLTGGDKLKR"
     aSDomain        10256..11152
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20335_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="1.80E-96"
                     /label="MOB22_RS20335_Condensation_Starter.1"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="303"
                     /protein_start="4"
                     /score="314.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHVISDG"
                     /tool="antismash"
                     /translation="FYPLTDAQKRIWYTEKFYPDTSISNLAGIGKLVSTEKIDWKLVEQ
                     AIQAFIRRNDAMRLRLRLNENGEPVQYISEYRPVDIKHTDTTGDPNAIESISQWGREET
                     KKPLPLYDCDLFRFSLFTIKENEVWFYANVHHVISDGISMNILGNAIMHIYLELAGGSE
                     TKEGISHSFIDHVLSEQDYAQSKRFEKDKAFWNKQFETVPELVSLKRNVSAGGSLEAER
                     FSKDVPEVLHQQILSFCEANKVSVLSVFQSVLAAYLYRISGQNDVVTGTFMGNRTNAKE
                     KQMLGMFVSTVPLRTNID"
     aSModule        10256..13375
                     /complete
                     /domains="nrpspksdomains_MOB22_RS20335_Condensation_Starter
                     .1"
                     /domains="nrpspksdomains_MOB22_RS20335_AMP-binding.1"
                     /domains="nrpspksdomains_MOB22_RS20335_PCP.1"
                     /locus_tags="MOB22_RS20335"
                     /monomer_pairings="Glu -> Glu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       10259..10297
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0001"
                     /evalue="7.30E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="18"
                     /protein_start="5"
                     /score="9.3"
                     /tool="antismash"
                     /translation="YPLTDAQKRIWYT"
     CDS_motif       10364..10441
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0002"
                     /evalue="1.20E-04"
                     /label="C2_starter_024-063"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="66"
                     /protein_start="40"
                     /score="14.8"
                     /tool="antismash"
                     /translation="KIDWKLVEQAIQAFIRRNDAMRLRLR"
     CDS_motif       10655..10720
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0003"
                     /evalue="1.90E-06"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="159"
                     /protein_start="137"
                     /score="20.5"
                     /tool="antismash"
                     /translation="VHHVISDGISMNILGNAIMHIY"
     CDS_motif       11051..11140
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0004"
                     /evalue="1.00E-11"
                     /label="C5_starter_265-294"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="299"
                     /protein_start="269"
                     /score="37.1"
                     /tool="antismash"
                     /translation="DVVTGTFMGNRTNAKEKQMLGMFVSTVPLR"
     CDS_motif       11192..11305
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0005"
                     /evalue="7.30E-10"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="354"
                     /protein_start="316"
                     /score="31.4"
                     /tool="antismash"
                     /translation="KDLMKTLRHQKYPYNLLINDLRETKSSLTKLFTVSLEY"
     aSDomain        11642..12844
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20335_AMP-binding.1"
                     /evalue="9.90E-114"
                     /label="MOB22_RS20335_AMP-binding.1"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="867"
                     /protein_start="466"
                     /score="372.0"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="FQEQAEQLSDHPAVVFEDRTLSYRTLHEQSARIANVLKQKGVGPD
                     SPVAVLLERSERMITSIMGILKAGGAYVPIDPAFPAERIQYILEDCGADFILTESQIEA
                     PAADAELIDFDQAIAEGAGESLNADVNARNLAYIIYTSGTTGRPKGVMIEHRQVHHLVV
                     SLQQTIYQSASQTLRMALLAPFHFDASVKQIFASLLLGQTLYIVPKKTVTNGSALAAYY
                     RRHCIEATDGTPAHLQMLIAAGDFEGLKLKHLLIGGEGLSSVVADKLLKLFKEAGIAPR
                     LTNVYGPTETCVDASVHPVIPENAVQSAYVPIGKALGNNRLYILDQKGRLQPEGVAGEL
                     YIAGDGVGRGYLHLPELTAEKFLQDPFVPGDRMYRTGDVVRWLPDGTIEYLGREDDQVK
                     VR"
     CDS_motif       11828..11869
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0006"
                     /evalue="2.10E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="542"
                     /protein_start="528"
                     /score="17.7"
                     /tool="antismash"
                     /translation="MGILKAGGAYVPID"
     CDS_motif       12047..12106
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0007"
                     /evalue="8.20E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="621"
                     /protein_start="601"
                     /score="33.6"
                     /tool="antismash"
                     /translation="AYIIYTSGTTGRPKGVMIEH"
     CDS_motif       12497..12520
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0008"
                     /evalue="1.40E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="759"
                     /protein_start="751"
                     /score="5.6"
                     /tool="antismash"
                     /translation="YGPTETCV"
     CDS_motif       12638..12727
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0009"
                     /evalue="9.20E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="828"
                     /protein_start="798"
                     /score="49.9"
                     /tool="antismash"
                     /translation="PEGVAGELYIAGDGVGRGYLHLPELTAEKF"
     CDS_motif       12809..12874
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0010"
                     /evalue="4.10E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="877"
                     /protein_start="855"
                     /score="38.0"
                     /tool="antismash"
                     /translation="YLGREDDQVKVRGYRIELGEIE"
     aSDomain        13172..13375
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20335_PCP.1"
                     /evalue="2.10E-24"
                     /label="MOB22_RS20335_PCP.1"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1044"
                     /protein_start="976"
                     /score="77.5"
                     /tool="antismash"
                     /translation="EKAIAAIWQDVLNVEKAGIFDNFFETGGHSLKAMTFLTRIHKETG
                     AEIPLQYLFEHPTIAALAEEADH"
     aSDomain        13418..14278
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20335_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.70E-105"
                     /label="MOB22_RS20335_Condensation_LCL.1"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1345"
                     /protein_start="1058"
                     /score="343.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHLISDG"
                     /tool="antismash"
                     /translation="QEHYPLSLAQQRTYIVSQFEDAGVGYNMPAAAILEGPLEIQKLER
                     AFQGLIRRHESLRTSFILENNTPRQNIHDSVDFKVELMERGGRSDEEIMTSFVRRFDLA
                     KAPLFRIGLMKLEENRHMLLFDMHHLISDGVSIGIILEELARLYKGEQLPELRLQYKDY
                     AVWQSGQAAEGYQKDRAYWKEAFAGELPVLQLLSDYPRPPVQSFEGDRVSIKLDAGLKN
                     RLNRLAEQNGATLYMVMLSAYYTLLSKYTGQDDIIVGTPSAGRNHSDTEGIVGMFVNTL
                     AIRSEV"
     aSModule        13418..16495
                     /complete
                     /domains="nrpspksdomains_MOB22_RS20335_Condensation_LCL.1"
                     /domains="nrpspksdomains_MOB22_RS20335_AMP-binding.2"
                     /domains="nrpspksdomains_MOB22_RS20335_PCP.2"
                     /locus_tags="MOB22_RS20335"
                     /monomer_pairings="Leu -> Leu"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       13493..13600
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0011"
                     /evalue="1.60E-15"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1119"
                     /protein_start="1083"
                     /score="49.4"
                     /tool="antismash"
                     /translation="YNMPAAAILEGPLEIQKLERAFQGLIRRHESLRTSF"
     CDS_motif       13796..13861
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0012"
                     /evalue="1.60E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1206"
                     /protein_start="1184"
                     /score="27.1"
                     /tool="antismash"
                     /translation="MHHLISDGVSIGIILEELARLY"
     CDS_motif       13892..13921
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0013"
                     /evalue="5.30E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1226"
                     /protein_start="1216"
                     /score="12.7"
                     /tool="antismash"
                     /translation="QYKDYAVWQS"
     CDS_motif       14180..14269
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0014"
                     /evalue="4.10E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1342"
                     /protein_start="1312"
                     /score="48.1"
                     /tool="antismash"
                     /translation="DIIVGTPSAGRNHSDTEGIVGMFVNTLAIR"
     CDS_motif       14318..14437
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0015"
                     /evalue="1.70E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1398"
                     /protein_start="1358"
                     /score="65.4"
                     /tool="antismash"
                     /translation="RTQVLDAFSHQDYPFEWLVEDLNIPRDVSRHPLFDTMFSL"
     aSDomain        14774..15967
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20335_AMP-binding.2"
                     /evalue="1.50E-110"
                     /label="MOB22_RS20335_AMP-binding.2"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1908"
                     /protein_start="1510"
                     /score="361.6"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FEEQAAKTPDQPALQYEGESLTYRELNERANRLARGILSLGAGEG
                     RTVAVLCERSMDMIVSILAVLKTGSAYVPIDPEHPIQRMQHFFRDSGAKVLLTQKKLKA
                     LAEEAEFSGVIVLADEEESYHADVQNLALPLDSAAMANLTYTSGTTGTPKGNIVTHANI
                     LRTVKETNYLSITEQDTILGLSNYVFDAFMFDMFGSLLNGAKLVLIPKETVLDMARLSR
                     VIERENISILMITTALFHLLVDLNPACLSTLRKIMFGGERASVEHVRKALQIVGKGKLL
                     HMYGPSESTVFATYHPVDELEEHTLSVPIGKPVSNTEVYIMDRAGHVQPAGIAGELCVS
                     GEGLVKGYYNRPELTEEKFVPHPFASGERMYKTGDLARWLPNGDIEFIGRIDHQVKIR"
     CDS_motif       14960..15001
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0016"
                     /evalue="3.10E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1586"
                     /protein_start="1572"
                     /score="14.2"
                     /tool="antismash"
                     /translation="LAVLKTGSAYVPID"
     CDS_motif       15200..15253
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0017"
                     /evalue="7.20E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1670"
                     /protein_start="1652"
                     /score="18.7"
                     /tool="antismash"
                     /translation="LTYTSGTTGTPKGNIVTH"
     CDS_motif       15623..15646
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0018"
                     /evalue="9.00E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1801"
                     /protein_start="1793"
                     /score="3.3"
                     /tool="antismash"
                     /translation="YGPSESTV"
     CDS_motif       15761..15850
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0019"
                     /evalue="2.30E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1869"
                     /protein_start="1839"
                     /score="42.3"
                     /tool="antismash"
                     /translation="PAGIAGELCVSGEGLVKGYYNRPELTEEKF"
     CDS_motif       15932..15997
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0020"
                     /evalue="4.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="1918"
                     /protein_start="1896"
                     /score="37.9"
                     /tool="antismash"
                     /translation="FIGRIDHQVKIRGQRIELGEIE"
     aSDomain        16289..16495
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20335_PCP.2"
                     /evalue="1.80E-29"
                     /label="MOB22_RS20335_PCP.2"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2084"
                     /protein_start="2015"
                     /score="93.8"
                     /tool="antismash"
                     /translation="EQKVADIWAHVLQAEQVGVYDQFFDIGGHSLAGMKMLALVHQELG
                     VELSLKDLFQSPTVEGLAQVIDSA"
     aSDomain        16538..17395
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20335_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.00E-108"
                     /label="MOB22_RS20335_Condensation_LCL.2"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2384"
                     /protein_start="2098"
                     /score="352.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="DTYPVSSPQKRMYVLQQLEGAETSYNMPAVLRLTGELDVERLNSV
                     MKQLMKRHEAFRTTFEIKDGETVQRIWEEAECEMAYFEAAEEETERIVSEFIKPFKIDR
                     LPLFRMGLIKHSDTEHVLLFDMHHIISDGASVGVLIEELSKLYDGETLEPLRIQYKDYA
                     VWQQQFIQSELYKKQEEHWLKELEGELPVLTLPTDYSRPAVQTFEGDRIAFSLEAGKVD
                     ALRRLAKETDSTMYMVLLASYSAFLSKLSGQDDIIVGSPVAGRSQADVSRVIGMFVNTL
                     ALRTY"
     aSModule        16538..18688
                     /domains="nrpspksdomains_MOB22_RS20335_Condensation_LCL.2"
                     /domains="nrpspksdomains_MOB22_RS20335_AMP-binding.3"
                     /incomplete
                     /locus_tags="MOB22_RS20335"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       16604..16717
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0021"
                     /evalue="5.10E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2158"
                     /protein_start="2120"
                     /score="51.0"
                     /tool="antismash"
                     /translation="TSYNMPAVLRLTGELDVERLNSVMKQLMKRHEAFRTTF"
     CDS_motif       16913..16978
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0022"
                     /evalue="7.30E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2245"
                     /protein_start="2223"
                     /score="31.3"
                     /tool="antismash"
                     /translation="MHHIISDGASVGVLIEELSKLY"
     CDS_motif       17009..17041
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0023"
                     /evalue="3.20E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2266"
                     /protein_start="2255"
                     /score="13.4"
                     /tool="antismash"
                     /translation="QYKDYAVWQQQ"
     CDS_motif       17300..17389
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0024"
                     /evalue="4.20E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2382"
                     /protein_start="2352"
                     /score="44.9"
                     /tool="antismash"
                     /translation="DIIVGSPVAGRSQADVSRVIGMFVNTLALR"
     CDS_motif       17438..17557
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0025"
                     /evalue="2.40E-12"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2438"
                     /protein_start="2398"
                     /score="39.2"
                     /tool="antismash"
                     /translation="KETALSAFDAQDYPLEDLIGNVQVQRDTSRNPLFDAVFSM"
     aSDomain        17894..18688
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB22_RS20335_AMP-binding.3"
                     /evalue="4.80E-65"
                     /label="MOB22_RS20335_AMP-binding.3"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2815"
                     /protein_start="2550"
                     /score="211.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEETAQRHKDRPAVTFNGQSWTYGELNAKANRLARILIDCGISAD
                     ERVGVLTKPSLEMSAAVLGVLKAGAGFVPIDPDYPDQRIDYILQDSGAKLLLKQEGISV
                     PDSFSGDVILLDGDRTILSLPLDENDEENPETDTNAENLAYMIYTSGTTGQPKGVMVEH
                     HALVNLCFWHHDAFSMTEEDRSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIV
                     RLNDYFETHGVTITFLPTQLAEQFMELENTSLRVLLTGGDKLK"
     CDS_motif       18080..18121
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0026"
                     /evalue="2.30E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2626"
                     /protein_start="2612"
                     /score="14.5"
                     /tool="antismash"
                     /translation="LGVLKAGAGFVPID"
     CDS_motif       18323..18382
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB22_RS20335_0027"
                     /evalue="2.40E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="MOB22_RS20335"
                     /protein_end="2713"
                     /protein_start="2693"
                     /score="32.2"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMVEH"
     assembly_gap    18692..18701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18702..>19218
                     /locus_tag="MOB22_RS20340"
                     /old_locus_tag="MOB22_20290"
     CDS             18704..>19218
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="MOB22_RS20340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_20290"
                     /product="AMP-binding protein"
                     /protein_id="WP_268430117.1"
                     /sec_met_domain="AMP-binding (E-value: 7.8e-39, bitscore:
                     123.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVYILGEGNQVQPEGVAGELCVAGRGLARGYLNREDETAKRFVAD
                     PFVPGERMYRTGDLVKWVNGGIEYIGRIDQQVKVRGYRIELSEIEVQLAQLSEVQDAAV
                     TAVKDKGGNTAIAAYVTPETADIEALKSSLKETLPDYMIPAFWVTLNELPVTANGKVDR
                     KALPEPDIE"
ORIGIN
        1 tctgatcatc ctcttgaaaa aaaactagta acggatatca cgtatttgcc ttatggacag
       61 aaacaattgt acctttccag tatattggat gtatacaatg gagaagtgat tgctttcacg
      121 attggagata agcaggacac agactttgtc ttaaacacac ttgatcagct gccaacactg
      181 cctcagaact gcgtgttaca tagtgaccaa ggatctgtgt atacatctta tgagtatcag
      241 aaagctgtta aaacaaaagg cattaccatg agcatgtccc gcaaagggac gcccgctgat
      301 aatgcctcca tcgaatcgtt tcattcctca ctaaagtctg aaacgttcta tcttaacagc
      361 attgatcgaa ccacgaccgc catcgtagaa cgcactgtca tagaatacat tcattattat
      421 aacaatattc gtattcaaac caaactaaac aaccaatcac cgataaacta tcggcaattg
      481 gctgtttaaa aggtgttttg acccctgtct caaaaacggg ggtcagtccc ctgcggagcg
      541 gcttttttta atgaaacgtt tgtttaaaca tagtgagcgt attttttctc aaggatattc
      601 tgaaaagccg cggcaattga gcaaatggcc caataaatga gagcggtcag aatatacatg
      661 gtcatgtaat caaactctct gccgccgatg attttcgcat gctggagcaa ttccggcact
      721 gtaatcatcg cagcgagaga ggacgctttg attaagtcga gcaatacatt ggcaagcgga
      781 ggcagcgcaa tacggatcga ctgcggcaaa atgatgcctc tcatcgactg ccaataggaa
      841 agtccgagag aggatgcggc ttcccactgt cctttttcaa cagatgaaat ggctgaacgg
      901 ttaatttccg caatgtaggc ggcgctgtta aggctaaagc caattaaagc ggccgtgaca
      961 gcggaaaatt caatgccgat ataaggaaat ccgaaataaa ggataaacag gatcaccaaa
     1021 atcggaacgc cccgcataaa tgagatatag agcttcgccg gccatcttaa caaggcaagc
     1081 tgagacattc tggcgagtga tatgaacagg ccgatcaccg ttcccgcaaa catgctcaca
     1141 aaagagatga gcagcgtata cccaatgcct cttatgacat aaggaaacga ttcaattgcc
     1201 agcttcgtgt taaaaatata ttcccactga atgctattaa tcatggcgtc cgattacaaa
     1261 tcaacgtcct gaacgtcgac atccagtttt tttgatacat ctgctttatt gaagaattgt
     1321 tccgacagct tagtcaaagt gccatccttg ctcatttctt tcaatgtttc gttcatcttt
     1381 ttctgaagtg ctgcattgct ttttttcata acaagtgcct gtttgttcgg catgtacttg
     1441 atgtcagggt ggatcgtaat gttgagatcc gggaatgctg cgagcgcaag tgtctgcagg
     1501 taatagtcgt ttaaaatgac atcagtgcgt ccgttggcga catcttttaa atactgctcg
     1561 ttggtcgcat tatcataaat cacttctttg gcgccgtatt ttctcgctac atccatgtag
     1621 actgtcgtag ctgcgccagc cgctttcttc ccttttaaat ctttaagcgt tttaatccct
     1681 gataaatcgt cttttcggac aattgctgtg ccgtatgaat atttatatgg agtggaaaac
     1741 gcgaacttct tctcgcggtc cttcgttaca tcgatgtcat ttgcggctgc gtcaacctgt
     1801 ccgctgttca ccgctgtcag catcccgtca atgcccatct ccttgaactc aaccttcagc
     1861 cccaggcgtt ttgccgcttc acgaacgact tcaacctcgt agcctgtcag cttatcggaa
     1921 ccagaatcgg tatcgtggta agaagtagga taaagcgtgc cggatgtagc gacaacgatt
     1981 ttccctttgt ctttgatctg ttcccagcct gtatccttgg aaccggcttc atttttgccg
     2041 gaacaggctg tcaatataag aaagaaagcc attgtaaaac tgaatataac ggctttggat
     2101 tgcatgaatg atttcacctt tgtttccccc aatctgttaa tcaactaaaa agatagcatt
     2161 gaacataagg ggttgcaata tgttgatgac gttttcaaaa agttcgaatt gtgtgtacaa
     2221 aagcgtgtga gcgtttaaaa gcggctatga agggaaggag tgataaaaaa ggagttgtct
     2281 aaggatgaaa gcactgacac atcaagggaa agagcatgtg aaagtgaagg aagtagcagg
     2341 ccccgaaatt agccaatact gtatgaaatg gtgaagaagc atgtagttga tcttctgcat
     2401 aatttaagtc ttgctgatgt ccataaggca tatgatatat tcgataagaa aaaaatggaa
     2461 accatcaaag ctatattgaa aacataatgg gggagtcaaa tgaatattta caaaccagcc
     2521 ggattctgga tcagactcgg agcttcattg ctggattaca ttattgtctc ggtgcctctt
     2581 ttgctgatct attggctgat tacaggaaaa gacccgaatg acagcatgtt catcagtttg
     2641 attgttctgc tgtactcaat tttgctgccg gtgttttgga acggctacct tatcggaaaa
     2701 agaatctgcg gaatcagaat cgtaaaaaaa gacggatcac aggtcagcct gttaacgatg
     2761 tttttgcggg ttattgttgc cggcctggtt tacggcatca ccttcggact cggcttgatc
     2821 gccagcctga ttctgatcgg cgtacgagaa gacaaaagaa cactgcatga tttaatcgcg
     2881 ggaacgtatg tcacttacgc gtcaccgggt gaagaagaac ttgaaaatga cgaacagatc
     2941 agaaagagcg aataatggtt cgctcttttt attttgtatg cataaaaaat gcaatgcagc
     3001 aaaacatatg aaaaatagga ttggaggaat aaattggatg aaacaaatga aaatcaatat
     3061 catcataatg ttcattgccg cggctgtcat ttctctgacc ggcacagctg aagctgctga
     3121 aaaacagcag cagcctcccg ctaatgtttc actgacagat cagcaaaaga aagaaattga
     3181 acagctggaa gcagacatac taaaaaagcg caaagacgta atctcaaaat atgtgcaata
     3241 cggcgtcctg cctaaagaaa gaggagaaca catcaaaaat cacatggaca aacactttga
     3301 aatgatgaaa caaaacggct ttgttccaaa gccccatcct catgcgcata aatttgaaaa
     3361 aaggcattaa gacggtaagc atccaatgga aattgggtgc ttttgagttt tttgcaaatg
     3421 aaaatattgg ctgagctgtt ccgcggctaa tatcgagttg atctgaaacg cgtttcatag
     3481 cctatgatag gtttatcaat agttcaggag tgataaacac tatgatccac cagcatccag
     3541 aatcttttcc gaaacatttt ttgtggggtt ctgcttcggc agcgtaccag attgaaggcg
     3601 cttggaacga agatggcaaa ggcccttctg tatgggacgt gtttacgaaa ataccgggca
     3661 aaacgttcaa aggaactaat ggcgatattg cggttgacca ttatcaccgt tataaagaag
     3721 atgtcgcttt aatggctgaa atgggcttaa aagcgtatcg attttccgtc agctggccgc
     3781 ggatttttcc aaaaggaaaa ggagaggtca atgaagcagg cctagcgttt tacgataacc
     3841 tgattgatga gttgctttct cataacatcg agccggttct gactttatat cactgggatt
     3901 tgcctcaggc gcttatggac gagtatggcg gctttgaatc aagaaatatc atcgaggatt
     3961 ttaataacta ttgtattact ctttataaac gttttggaga cagagtaaaa tattgggtta
     4021 cgttaaacga acaaaactac aattttaatc acggatttat tacagccatg catccgcctg
     4081 gagtgaaaga tagaaaacga ttttacgaag caaaccatat agcgttcctg gctaatgcga
     4141 aggccattga atcttttagg gaatatgtgc ctgaaggcaa aataggtcca agctttgctt
     4201 attctccggc atatccttta tctagccatc cagaagacat actggctttt gaaaatgctg
     4261 aagagtttat gaacaattgg tggcttgaca tgtattgctg gggaacctac ccgcaaattc
     4321 ctttccgcta tttagaaaaa cagggctggg cgccgacaat caaagcggga gatatggagc
     4381 tgcttgccaa agggaagcct gattttgtag gtgtcaatta ttatcagacc atcacctacg
     4441 aacgaaatcc gcttgatggt gtgtcagaag gaaaaatgaa tacaacagga caaaagggga
     4501 ccaaccagga aacagggata cccggagtat ttaaaacgaa aaaaaatccg catctgacaa
     4561 cgagcaactg ggattggaca attgacccaa ttggattgcg tatcggcctt cgacgaatta
     4621 caagccgcta tcagcttcca gtgtttatca cagaaaacgg tttgggtgag tttgataagg
     4681 ttgaagacgg cactgtacag gatgattatc gaattgatta tttgcgttcg catcttgagc
     4741 aatgcagaca agccatcagc gacggtgtag atttgatagg ctattgcagc tggtcgttta
     4801 ctgatctgtt aagctggctg aatggctacc aaaaaagata cggatttgta tatgtcaatc
     4861 gcgatgaaga aaatacacat gatttaaaac gtttgaagaa aaaaagcttt tattggtatc
     4921 aggatgtaat caagacgaat ggagaaagtt tataaagagt ccctgagagt cactctctca
     4981 gggctttttc attacacaga aacagcatca ataatataag tgtcggcgtg cagatctgac
     5041 ttctcagccg gcacagtcca gcatgtcaat tgatagtccc ctttaggaag atcaaaggac
     5101 agccttttag acataatgct ttcaatctca attccgtttt ctgtgacatg aaaaggaaca
     5161 gtcacttttt tctcgtagga gtttacagtt tctgaactgt taaggcgcaa aagaataaat
     5221 gcctttgtat ttctctgagc ctcaaaggaa attgctccgt ccgccgcagc atatcctttt
     5281 tcaatcgctt cgtcagtcca atccatgaca ggaggtgcgg aatccttttg aaatacagta
     5341 aattgatgat atgaaatcga cagttcttgc ggcttccatg acttgatcaa tgtgctgttc
     5401 ctcctctttt aactgtttac tgaatcgtga ataaaacctt tgtgccgtca gggtaatcgg
     5461 taagctgatg cccgacccaa gaacctgcac cgcggttgtc agcgggagaa atatattcta
     5521 cagaagctcc ctcaccgcct tctttgcaca tggccattgg ccattcatct ctgtcatacc
     5581 ccgttttagg cggtacgtct tttaatgatt gctcacggcg ttcctcagct ccgtccctgt
     5641 cgatggtaca cacatctgag tgcccctcat ttatcgcatc ctttatatgt tttgccgttt
     5701 cgggataacg gtcagaaggg aaggcgattg tttcgtcata ttgtttcgtt tgagacgttt
     5761 tttgatcagc tgagaaaaag tctcccttta tcaggctgac agctgcagct gctatgacga
     5821 cgatgacgag aagtatcgtt tttatgatgt ccatcgtgat gttcacctcc cgcaattagc
     5881 aactttaaaa actgatccaa gagtaatcca gaaagcgttt agtgtaaagg ttaaaatttt
     5941 atgttttaca ctcagtttat tgcagaaatc gtaattttag ctggtcgaaa tatttgtttt
     6001 cagtaaaata ccgttgccaa tttacaataa tgatagtaag tcatcggccg ttttcaggtt
     6061 gatcaatcat tttaaattgg cttatgatac cggtaagctc ctcagctaat tgagatagcg
     6121 tttcagaagc tgcagtaatc tcttccatcg cagcaaattg ctcttctgtt aatgcagcgg
     6181 cctgccgggt attttctgta gattctttaa tgtcagccgt attggctgcg aaggaatcgt
     6241 ttatttgatg cgcactggct gaaatgtttg atacagatgc tgataaatca ctgatcttgg
     6301 cactgatttc acctgttgcg gctgcaattt ccttaaatgc atcttttgtg cgctggatca
     6361 tggtgacacc ttcagcagcc tctgttttta catgctcaac tgaccttgca gattggttca
     6421 tatctttttg tatctctgta attaatgttg aaatttgtcc tgcagactgt tgagactctt
     6481 ccgctaattt acgtacttca tcggcaacaa cagcaaagcc tttgccatgt tcaccggctc
     6541 tggcagcttc aatcgctgca ttcagcgcaa gcaggtttgt ctgatctgca atttgggtga
     6601 tgacggataa aatttgttca atttgtttag agcggccgtc cagctgctgt atgatttctc
     6661 cgcttttctg aatcgaatgg tgtatcgcat ccatttgtgc ctgtacatct gcaatttcct
     6721 tttgcccgat gtcggctttt gattgcgcaa gctgtccttt gtcagcaata gcagcagtat
     6781 ttgaagaaat atctcgtatg tctgctgaag cttgtttcag cgagctttca ctgttttcga
     6841 ttcgggtgat ctgatgctgg gctccatttg caatgtgctg tacagcttct gtgatctttt
     6901 ctgaagcttg atttgtttcc tctgctccgg ctgagagctg ctgggaggct gacgcaagct
     6961 gaagggctga ttgctgcacg gtttgaatgg tattgttcag tttcattctc atcttattgt
     7021 aatagacgct gagttctgat agctcgtcac cggatttgtc tgatacctct attgtcatat
     7081 ctccgtttcc agcactttca aaggcacatt ttaaagcgtt tagccgctta ttgattcttc
     7141 tggtgaaaag caggacaagg atgactgata caataatgac gatagcaaga acgatagcga
     7201 attgagtgaa gagtgacttg gtaagcgatg ctaatatgct ttggttggct ccggtataca
     7261 gcatgccgat gatgtccccg ttttgatctt ttaaaggcat gtatgccgtc tgataagagg
     7321 agcctgccac gtctgcttgt ccgtagaatc gttttccgtt ctttaaaaca gcgtcaataa
     7381 cttcagaaga agcctgagtg ccgacagctc tttcgccgcc cttcatgaca ttggttgcaa
     7441 cacgagtatc tccttggaaa atggtaaccg tatcacctgt tttttcacca agcagatcaa
     7501 caatatcttc atttccgttg atttggatct tgcctttata cagcttattg ttttttacct
     7561 gccagtcccc tgaaatgaca tcatcaatat aagcgctgct taaagcgaga tcgcctttcg
     7621 ctttttcagt cgccatttgt ttcatgcttt ctgtaatttc cttaagcatt acagtaccga
     7681 ctgataccga aaacaaaaga ataatcacga aaaccatgca gagaattttt gttcctagct
     7741 taagctttag ttgttttatc aacataattc ctcctttttg tgatttgtag gtcatattac
     7801 ttatcgggtt tttaggttta aagctttatt tagaaaataa taaaaccggc ctgatctaca
     7861 ggccggttgt gtgacgctat tcaaggtttg cgtggtgggt gaacatggtt tcagaatcga
     7921 gtcctttttt ctccatgagt tttaaaatca ctgcatcata gaatagcagc aatgtttgct
     7981 caaataagga acccattggc tgaatggttt tatagcttcc gtttgaccgg tctttagggg
     8041 acccaggcat tttgacgatg agatccgctt gttttccgat gcttgattcc ggattgatgg
     8101 ttaaagcggc aacgatcccg tgtaagcttt ttgcttttgc tgctgtatgg attaagctct
     8161 ttgtctcgcc ggatcctgag ccgataatga cgagatctcc ctcgcggagc ggcggagtga
     8221 gaatctcacc ggcaatatga gcgttgaagc ccatgtggat caatctcatc gcgaaggatt
     8281 ttgccatcag gccagaccgc cccgcccctg cagtgaaaat ttgatgggac gaaagaatgt
     8341 gatcggcgag ctgatcagct tcttcattag aaatataagc cgctgaacgg tgtaactcat
     8401 tgagaatttc cgttacgtat tcagtcgttt tcatatgatt tatccttggg caataagctg
     8461 tttcattttt gaagctgttt ccgctttatc ggctgcgctt gtaattccgc ccccaacaat
     8521 gacaaggtca ggattttgtt tgatcacttc tggcagtgta tcaagtttga tcccgcccgc
     8581 aattgcggtt tttgcgtttt taacggtatt tttgattgtc gttaattctt cgaaagagtt
     8641 tttgccctct gcttgaagat catatccagt gtggacgcag atgtagtcaa caccaagtgc
     8701 gtcaatttct ttcgcacggg attcaatatc tttcacgtta atcatgtcta ccaagatttt
     8761 cttcttctgt tttttggctt ctcctactgc gcctttaatc gttgcatcgt ctgtagcccc
     8821 taaaacggtg ataatgtcag cgcctgcttc agatgctttc atgatttcat atccgccggc
     8881 atccatgatt ttcaggtctg ctaaaacctt caattgcgga aatgcttctt ttatttcttt
     8941 aacggctcta aggccctcat taatgacaac cggtgttccg atctcaacca cgtcgatgta
     9001 ttgttccact tctttgacaa gctcgatggc ttccgggatg ttgacgaggt ctaatgccag
     9061 ctgtaattcc attgttgatc cactcccttt ttcgaattat ttttcattct ataggttcac
     9121 tcttttgatg agaagtaggc actttgaagt caactagtta cttggaggat actgtgagta
     9181 gaggcaatca gatttctttc gcttggtttt aggagtgatt ataataaaaa ggagtctgca
     9241 aaaaagtaag taagtgatga tacgtatcta acatacaaaa aatatactaa ggggggattt
     9301 cttttgagcc ggatggacga caaaaggttt aattgtgaga aggaattaac gcttgcggtg
     9361 atcggcggta aatggaaaat gctcattttg tggcatttag gaaaagaagg cacgaaacgg
     9421 ttcaatgaat taaaagcttt gattcctgat attacgcaga agatcctcgt gaatcagctg
     9481 agagagcttg agcaggatat gattgttcac agggaagtgt atccggttgt cccgccgaag
     9541 gttgaatact ccttgacccc gcaaggagaa agcctgatgc ctattcttga tgccatgtat
     9601 gagtggggga aaggctatat ggagctgatt gacatcgata aaaatgtcat aaaggaatca
     9661 ttgtaggcgc tcttgaataa gggtgtcttt ttttttgcct gtttttcggt ttttgcgcgg
     9721 taaacatatg ggatgtaaag attgtaaatg gctttcaaca ataaatggga tgaagcggaa
     9781 aggatcattt taaaaatttt tatttttctg taaataatgt ttagtggaaa ttattgcggc
     9841 atcccgcaaa aatatcccag taaataaact ggaatctttc ggtatcccgc atgaaacttt
     9901 tcacccattt ttcggtgata aaaacatttt tttcatttaa agtgaacggt agtaaggtaa
     9961 aaaaatattg aaaacaatga ataattagcc aaaattgtat cgtgtttgga tgagagatct
    10021 tgcggtcttt atttgcttgt gttaaacccc gcaatgatga ttgatggcag ttgttttgac
    10081 agcggcaatc tgtttttttg tttggatgca ctgcttttta aacgtagtac tttgggcttt
    10141 ttcggctgtt agttcataag gattaaaagc ttatatggat aagaaaggga aaatatgctg
    10201 cacatgttca ctggttattg aagattaggg gaggtatgac aatatggaaa taacttttta
    10261 ccctttaacg gatgcacaaa aacgaatttg gtatacagaa aaattttatc ctgacacgag
    10321 tatttcaaat cttgcgggga ttggtaagct ggtttcaact gaaaagattg attggaagct
    10381 tgtggagcag gcgattcaag cgtttatccg cagaaatgac gccatgcgcc ttcgtttgcg
    10441 tctcaatgaa aacggagagc ccgttcaata tattagcgag tatcggcctg ttgatataaa
    10501 acatactgac accactggag atccgaatgc gatagaatct atttcacaat ggggccggga
    10561 ggaaacaaaa aaacctctgc cgttgtacga ttgtgacttg ttccgttttt ccttgttcac
    10621 cataaaggaa aatgaagtat ggttttacgc aaatgttcat cacgtgattt ctgatggaat
    10681 ttccatgaat attctcggaa atgcgattat gcacatttat ttggaattag ccggcggctc
    10741 agaaacaaaa gagggcattt cgcattcatt tatcgatcat gttttatctg aacaggacta
    10801 tgctcaatca aaacggtttg aaaaggacaa ggcgttttgg aacaagcaat tcgaaacggt
    10861 gcctgaactt gtctccctga aacggaatgt ctctgcagga ggaagtttag aggctgagcg
    10921 gttttctaaa gatgtgcctg aggtgcttca tcagcagatt ctatcgtttt gtgaggcaaa
    10981 caaggtcagc gttctttcgg tgtttcaatc ggtgctcgcc gcctatttgt acagaatcag
    11041 cggccagaat gatgtcgtga cgggaacctt tatgggcaac cggacaaatg cgaaagaaaa
    11101 gcagatgctt ggcatgtttg tatctacggt tccgctgcgg acgaacattg acggcgggca
    11161 ggcgttctta gattttgtca aagaccggat gaaggatctc atgaaaacac ttcgtcacca
    11221 aaagtacccg tataacctcc tgattaacga tttgcgtgaa acaaagagct ctctgaccaa
    11281 gctgtttacg gtttctcttg aatatcaggt gatgcagtgg cagaaagaag agaatctggc
    11341 ctttttgact gagccgattt tcagcggcag cggattaaat gatgtatcga ttcatgtaaa
    11401 ggatcgatgg gatactggga aactcaccat tgattttgat taccgcactg atttattttc
    11461 acgtgaagaa atcaaaattg tttgtgaacg catgattacg atgctggaga acgcattatt
    11521 tcatccagat cataccattg atgaattaac gctgatttct gaagcggaga aagagaagct
    11581 gcttgtgagg gccggcggtg aatctgtcag ctatcgtaag gatatgacga ttccagagct
    11641 gttccaagaa caggctgagc agctttctga tcatccagcg gttgtatttg aagatcgcac
    11701 attgtcctat cgaacgttac atgagcaatc tgcacgcatc gccaatgtgc tgaaacagaa
    11761 aggcgttggc cctgacagtc ctgttgcggt tctgcttgaa cgctctgaac ggatgattac
    11821 gtctatcatg gggattttaa aagccggcgg agcctatgtg ccgattgatc ccgcttttcc
    11881 tgcggagcgc attcaatata ttttggagga ctgcggagcg gacttcatcc tgactgaatc
    11941 gcagattgaa gctcctgcgg ccgatgctga attgattgat tttgatcagg cgattgcgga
    12001 aggtgctggc gaaagcctga atgcagatgt aaacgcacgg aatctggcct atattattta
    12061 cacatcggga acaaccggac gtccgaaagg cgttatgatc gagcatcgcc aggttcatca
    12121 tttggttgtg tctttgcagc agacgattta ccaaagcgcc agccaaacac tgcggatggc
    12181 attgcttgcg ccgttccact tcgatgcgtc agtgaagcag attttcgcgt cgcttctttt
    12241 gggacaaacc ctttatattg taccgaagaa aacagtgacg aacggatctg ccctagctgc
    12301 gtattatcgc aggcactgca ttgaggcaac ggacggaaca ccggcccatc tgcaaatgct
    12361 gatagcagct ggcgattttg aaggcttaaa gctgaagcac ctgctgattg gaggagaggg
    12421 tttatcgtct gtcgttgcgg acaagttgct gaagctgttt aaagaagccg gcatagcgcc
    12481 gcgtttgacc aatgtgtacg ggccgaccga aacgtgtgtg gacgcatctg ttcatccggt
    12541 tatcccggag aatgctgttc aatcagcgta tgtgccgatc gggaaagcgc tggggaacaa
    12601 ccgcttatat attttggatc aaaaaggccg gctgcagccg gaaggcgtgg cgggcgagct
    12661 ttatatcgcg ggcgatggtg taggccgagg ttatttacat ctgcctgaat tgacggctga
    12721 gaagttttta caagatccat tcgtaccagg cgatcggatg taccggaccg gagacgtggt
    12781 gcgctggctt ccagatggaa caatcgaata tttaggaaga gaggatgacc aggtcaaagt
    12841 ccgcggatac cggattgagc tcggggaaat tgaagccgtt attcagcagg cgtctgacgt
    12901 tgcgaaagcc gttgttttgg cacgtcctga cgaacaggga aatctcgagg tcagcgcata
    12961 tgttgtgcag aagcctggaa gcgaatttgc tccagccagc ctgcgggcac acgcggccag
    13021 acagcttcct gactacatgg tgccggctta ctttacagaa atcacagaaa ttccgctcac
    13081 gccaagcggc aaagtcgacc gccgcaagct gtttgcacta gaggtgaagg cggtcagcgg
    13141 aacggcctat acggcgccgc gaaatgagac ggaaaaagca atcgcagcca tttggcagga
    13201 tgtgctcaat gttgaaaaag cggggatctt tgacaatttc tttgaaacgg gcgggcattc
    13261 attgaaggca atgacgtttt taacaaggat tcataaggaa acaggcgctg agattccgct
    13321 tcaatatttg tttgagcatc cgacgattgc cgctcttgca gaggaagccg atcacagaga
    13381 aagccgggca tttgcagcaa ttgaacctgc tgaaaaacag gagcattacc cgctttcatt
    13441 ggcacagcag cgaacatata tcgtcagcca gtttgaggat gcgggagtcg gatacaacat
    13501 gccggcggca gcgattttgg aaggaccttt agagattcaa aagctggagc gggcatttca
    13561 agggttaatc cgccgccacg agtctctgag aacgtcattt attcttgaaa acaacacgcc
    13621 tagacagaac attcatgaca gtgtcgattt caaagtcgag ttgatggaaa gaggcggccg
    13681 ctccgatgag gaaattatga cttcattcgt ccggagattt gatttggcga aagcgccgct
    13741 gttcagaatc ggcttgatga agttagaaga gaaccgtcat atgctgctgt ttgacatgca
    13801 ccatttgatt tctgacggtg tgtccatcgg cattattttg gaggagttag cacggttata
    13861 taaaggcgaa cagcttcctg agcttcgtct tcaatataag gattatgccg tgtggcaaag
    13921 cggacaggct gctgaagggt accagaagga ccgggcgtat tggaaggaag cctttgcggg
    13981 cgagcttccg gtgcttcagc ttctgtccga ttacccgaga ccgcctgttc aaagctttga
    14041 aggagaccgg gtgtcaatca agctggatgc ggggttgaag aatcgcctaa atcgtttggc
    14101 tgaacaaaac ggcgccactt tgtatatggt gatgctgtcc gcttactata cgcttttatc
    14161 aaagtataca ggacaggatg acattattgt cggcacgccg tcagcgggca gaaaccactc
    14221 cgatacagag ggcattgtcg ggatgttcgt caatacgctc gcgattcgca gcgaagtgaa
    14281 gcaggatgag acgtttaccg atttgatcac gcgtgtccgc acacaggtgc tggatgcttt
    14341 ttctcatcag gattatccgt ttgagtggct tgttgaagat ttgaacattc cgcgtgatgt
    14401 aagcagacat ccgctgtttg acacgatgtt cagtcttcaa aatgcgacag agggcattcc
    14461 ggctgtcggc gatctgtctt tgtctgttca tgagactaac ttcaagattg caaaatttga
    14521 tttgacgatg caggcaagag aaaccgatga gggcattgag cttgatttgg actacagcac
    14581 gaagctgttt aaacaaagca cggcagacag gctgcttacg cattttgcgc gtttgcttga
    14641 agatgctgct gctgagcctg agaaacagat ttctgagtac aaacttcttt ccgaagagga
    14701 gactgcttcg caaattcagc agttcaaccc ggaaagaaca ccttatccga aagacaagac
    14761 aattgttcag ctgtttgaag agcaagcggc gaaaacgcca gaccagccgg cacttcaata
    14821 tgaaggcgaa tcacttacct atcgtgagct gaatgaacgg gccaatcggt tagcgcgcgg
    14881 cattctttct cttggggcag gcgaaggcag aacagtggct gtcttatgcg agcggtcaat
    14941 ggacatgatt gtgtcgatcc tcgcggtatt aaagacaggt tcggcttatg ttccgattga
    15001 tccggagcac ccgattcagc ggatgcagca tttcttccgc gacagcggag cgaaggtgct
    15061 tctcactcag aaaaaattaa aggctttagc agaagaagcg gaatttagcg gcgttatcgt
    15121 gcttgcagat gaggaagaaa gctatcatgc cgatgtgcaa aatcttgcac tgcctcttga
    15181 ttctgcggcg atggccaact tgacatatac ttccggaacg acaggaacac cgaagggaaa
    15241 tatcgtgacg catgctaata ttctgcgcac ggtgaaggaa acgaattatc tcagcattac
    15301 agaacaggat acgattctcg gactttccaa ttacgtattt gacgcgttta tgtttgatat
    15361 gttcggttct ttgttaaacg gtgccaagct tgtgctgata ccgaaagaaa cagttttgga
    15421 tatggctcgc ctgtctcgcg ttattgagcg ggagaacatc agcattctta tgattacaac
    15481 cgctttgttc cacctgcttg tggacttgaa tccggcgtgc ttgtcaacgc tgcgcaagat
    15541 tatgtttggc ggggagcggg cttcagttga gcatgtaaga aaagctttgc aaattgtcgg
    15601 aaaaggcaag ctccttcaca tgtacgggcc gtctgaaagc acggttttcg cgacgtatca
    15661 tccggttgat gaattggagg agcacacgct gtctgttccg attggaaaac cggtcagcaa
    15721 tacggaagta tacatcatgg accgggcggg acacgtgcag cctgccggga tcgccgggga
    15781 gctatgcgtc agcggcgaag ggctcgtgaa aggctattac aaccgtcctg aactgactga
    15841 ggagaaattc gttccacatc cgtttgcatc cggcgaacgc atgtataaaa cgggtgacct
    15901 tgccagatgg ctgccgaatg gcgatatcga atttatcggg cggatcgacc accaggtgaa
    15961 aattcgcgga cagcgcatcg agcttggaga aatcgaacat cagatgcaaa cccacgaccg
    16021 cgtgcaagag agtgttgtgc ttgctgttga tcaaggcgcg ggagacaaac tgctctgcgc
    16081 gtactttgtc ggagagggag aaatctcatc tcaagagctg agagagcatg ccgcgaagga
    16141 tctgccggct tatatggttc ctgcggtgtt tatccaaatg gacgagctgc cgctgaccgg
    16201 gaacggaaaa atcgactgga gagcgctgcc gcttcctgat gccaacgtct caagaggtgt
    16261 ttcatatgtt gcgcctcgca atgaaacgga acaaaaagtc gcagacattt gggcgcatgt
    16321 gcttcaggct gaacaagtcg gcgtttatga ccaattcttt gacatcggcg gacattcatt
    16381 agcaggtatg aagatgcttg ccttggttca tcaggaactg ggcgttgagc tgtcactcaa
    16441 agatctcttc cagtcaccga cggttgaggg cttggcacag gtgattgact ctgctgaaaa
    16501 agggacaatc atcagcatca gcccggcaaa gaaacaagat acgtatcctg tttcttcgcc
    16561 gcaaaaacgg atgtacgtgc tccagcagct tgaaggagcg gaaacgagct ataacatgcc
    16621 ggcggttctg cgccttacag gtgagcttga tgttgaaagg ctcaacagcg tgatgaagca
    16681 gttgatgaag cgtcatgaag cctttagaac cacgtttgaa ataaaagatg gagaaacggt
    16741 gcagcgaatc tgggaagagg ctgaatgtga gatggcatat ttcgaagccg cagaagaaga
    16801 gacagagcgg atcgtttctg agtttatcaa gcctttcaaa atcgatcggc ttccgctgtt
    16861 cagaatgggg ctgatcaagc attcagacac tgagcatgtg ctgctgttcg atatgcatca
    16921 tattatttct gatggggcgt ctgtcggcgt gctgattgag gagctttcaa agctgtacga
    16981 cggtgaaacc cttgagccgc tccgcattca atataaggat tatgctgtgt ggcagcagca
    17041 gttcattcag tctgagcttt acaagaagca agaggagcat tggctgaagg agctggaagg
    17101 agagctgcct gtgctgacgc ttccgactga ctatagccgg cctgccgttc aaacatttga
    17161 gggagaccgc attgcatttt cattagaagc gggcaaagtt gatgccctgc gcagacttgc
    17221 aaaagaaacg gattccacaa tgtacatggt gcttctcgct tcatacagtg cgtttttatc
    17281 aaaattgagc gggcaagacg atatcatcgt cggttcacct gtagccggac gatctcaagc
    17341 ggacgtcagc cgcgtcatcg gaatgttcgt caatacattg gcgctgcgca cgtatcctaa
    17401 aggtgaaaag acgtttgctg actatcttag cgaagtgaaa gaaacggcac tcagcgcttt
    17461 tgatgcgcaa gattacccgc ttgaggactt gatcgggaac gtacaggttc agcgtgacac
    17521 gagcagaaat ccgttattcg atgcagtgtt ttcaatgcaa aatgcgaaca tcaaggatct
    17581 aacgatgaaa gggattcagc tggagccgca tccgtttgaa cggaaaacgg ccaagtttga
    17641 cattacactg accgctgacg aaaccgatgg agggcttacg ttcgtactcg aatacaatac
    17701 agcgctgttt aagccggaaa cgattgaacg atggaagcaa tactggatgc agcttctaga
    17761 tgctgtcaca ggcaatccga accaaccgct tgccagcctg tcaatcgtca cggaaacaga
    17821 aaaacaaacg cttctggacg cctggaaggg caaaacgctg cctgtgccga cagacaaaac
    17881 cgttcatcag ctattcgaag agaccgctca gcgccacaaa gaccgcccgg ctgtcacatt
    17941 caacggccag tcatggacgt acggcgagct gaatgccaag gcaaaccgtc ttgctcggat
    18001 tctgatcgac tgcggcatca gcgcggatga gcgcgtcggc gttctcacga agccgtcgct
    18061 ggaaatgtcc gctgcggtgc tcggcgtctt aaaggcgggg gcggggtttg tgccgattga
    18121 tcctgattat ccggatcagc ggattgacta tattttacaa gacagcggcg cgaagcttct
    18181 cttgaaacag gaaggcattt cagtgccgga tagcttctca ggagacgtca ttcttctcga
    18241 cggggatcgt acgattctaa gcctgccgct agatgaaaac gatgaagaaa atccagagac
    18301 ggatacaaac gcggagaacc tcgcgtacat gatttacacg tctggaacga ccggacagcc
    18361 gaaaggtgtc atggtcgagc atcatgcgct tgtgaacctg tgcttctggc accacgacgc
    18421 gttcagtatg acagaggagg accgcagtgc gaagtacgcg ggcttcggat ttgacgcctc
    18481 catttgggag atgttcccta cctggacaat cggcgccgag cttcacgtca ttgatgaagc
    18541 gatccgcctc gatatcgtcc gtttgaacga ctatttcgaa acgcacggcg tgacgatcac
    18601 gttcctgccg acgcagctgg cggaacagtt catggagctt gagaacacat cactccgtgt
    18661 cctcttgact ggaggagaca agctgaagcg tnnnnnnnnn ngagagtgta cattctcggc
    18721 gagggcaatc aggtgcagcc ggaaggcgta gccggagagc tttgcgtggc gggacgcggc
    18781 ctggcgcgcg gctatctgaa tcgagaagac gaaaccgcga agcggtttgt cgctgatccg
    18841 tttgtcccgg gtgaacgcat gtaccgcacc ggcgatttgg tgaaatgggt gaacggcggc
    18901 atcgagtaca tcggccggat cgaccagcag gtcaaggtcc gcggctaccg gatcgagctc
    18961 tcagaaatcg aagtgcagct tgcccagctt tctgaggtgc aggatgcggc ggtgacagct
    19021 gtcaaagata aaggcggcaa tacagcgatc gcggcgtatg tcacaccgga aacagctgac
    19081 atagaagcat taaagtcttc gcttaaagaa accctgccgg actacatgat cccggcgttc
    19141 tgggtgacgc tgaacgagct tccggttacg gcaaacggaa aggttgaccg caaagccttg
    19201 ccggagccgg acatcgaa
//
