LOCUS       NZ_JALAMD010000004     10357 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Bacillus inaquosorum strain N18A14 contig_0000004, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAMD010000004
VERSION     NZ_JALAMD010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 497430)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 497430)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 223.65929725610533x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 03:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,188
            CDSs (total)                      :: 4,096
            Genes (coding)                    :: 3,969
            CDSs (with protein)               :: 3,969
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 4 (5S, 16S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 82 of 127
            Pseudo Genes (incomplete)         :: 72 of 127
            Pseudo Genes (internal stop)      :: 17 of 127
            Pseudo Genes (multiple problems)  :: 37 of 127
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:22:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 267138
            Orig. end                         :: 277495
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAMD010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10357
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10357
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10357
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5357](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5357
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            128..949
                     /locus_tag="MOB22_RS06845"
                     /old_locus_tag="MOB22_06825"
     CDS             128..949
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390467.1"
                     /locus_tag="MOB22_RS06845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06825"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="WP_268314902.1"
                     /transl_table=11
                     /translation="MAIKVVKNLVSKSKYGLKCPNPMKAEYITIHNTANDASAANEISY
                     MKNNSSSTSFHFAVDDKQVIQGIPTNRNAWHTGDGTNGTGNRKSIGVEICYSKSGGPKY
                     RAAEKLAIKFVAQLLKERGWGIERVRKHQDWSGKYCPHRILDEGRWSEVKAAIDAELKA
                     IGGKSSSKKTTSPKAVKKSSSSKKKTSFNLPSGIFKVKSPLMHSDAVKQIQTALAALHF
                     YPDKKAKNFGIDSYYGPKTADAVRRFQLMNGLNPDGIYGPKTKAKIKGLLK"
     gene            complement(988..1287)
                     /locus_tag="MOB22_RS06850"
                     /old_locus_tag="MOB22_06830"
     CDS             complement(988..1287)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014304563.1"
                     /locus_tag="MOB22_RS06850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06830"
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="WP_080529577.1"
                     /transl_table=11
                     /translation="MIDTNISEPVFQFLGGIFHQDIETPESALDEYLEEIPKEEQETDI
                     VALKDFINSDYTDEEKNDFIDEAADGVDIRSYGVSPLVWLEQVIQKIEKNIETL"
     gene            complement(1292..3172)
                     /locus_tag="MOB22_RS06855"
                     /old_locus_tag="MOB22_06835"
     CDS             complement(1292..3172)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015417244.1"
                     /locus_tag="MOB22_RS06855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06835"
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="WP_268314901.1"
                     /transl_table=11
                     /translation="MKVFEAKTLMSEATDRAKEYKELRTQMVNLRKALQSVAELDDSEF
                     SGKGANNIKAFYHDHVGVTDQWIDYIDMKIAFFNSIAGAVEDKGLSDAYIEESFLEHEL
                     ANAHKKSKSIMSEQKKAMKDILNDIDDILPLDLFSTETFKDELADANDKRKKTIEKLGD
                     LDEDLLTEYALSEPNEQFIKSDFQKLQEATGKGKNATPIHYNAKAYRESDIHKKKGDIE
                     KRTEAYLTIKKEEAKEREIKELKKKLADGVTDPDEYLEIAKEIGYENLEPAQLQYVVQL
                     EQAKQLEEVGESVINAGKATIDFIDGVNDGLNDAIDDTLYGVKDLVVGAWEYSQLPTEL
                     KLTKTITTVLSVPSYSKIIWTNLVDSWNDKMVNGDAYEKGHYISYAIGNIVGPKGAGAA
                     VQTTTKLSKVGKVVEGGTAAAHVNKGINKGKAYINSIVRNKNDLALVGIAQDIDNTYNA
                     KNTPLLKSIIEDKKESVLRKSETSNNMGIGNVKQGDSTPLAPGGGLAAHEAKGGHLIER
                     HVGKTDEELIQRLDKSKMSNKKISGSSSFKDRATAERVANSALTNNKAKIEKWLNSDVK
                     KPLVLSYKGNEVLGRGIPKGEDTITDLTNAKIILKKNKDGSFILTGYPTR"
     misc_feature    3316..3332
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.62"
                     /tool="antismash"
     gene            <3369..4487
                     /locus_tag="MOB22_RS06860"
                     /old_locus_tag="MOB22_06840"
                     /pseudo=""
     CDS             <3369..4487
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014479091.1"
                     /locus_tag="MOB22_RS06860"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06840"
                     /product="Rap family tetratricopeptide repeat protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="LPSSHVGVKINEWYKMIRQFSISDAEILKEEVTQKIERMEEDQDL
                     LIYFQLMCFRHQLMLDYLEPKNKYGDRPTIKELLEKIEAPQKKLTGLLKYYSLFFRGMY
                     EFDQKEYVEAINYYRKAEKELGYVTDEIEKAEFHFKVAEAYYIMKQTHVSMHHILQALA
                     VYDTHTLYQIRKIQSLFVIAGNYDDFKCYDKAIPHLKNALQIADKIYNKRMMIIANLNI
                     GLSYDRAGNYIEAALYFKEALRLIGEKESDLFTKVYFGLCCALFKSGHIMDGVPVLEKG
                     IEYLDKNPNELYRQLFDFLDALYVQGIYETKILEVLEYLDSKKLYAYIEECVKCVAVEY
                     EKANDHLKASRYFKAVLETQTKIQRGDCLYDY"
     misc_feature    complement(3376..3390)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.6"
                     /tool="antismash"
     gene            4477..4638
                     /locus_tag="MOB22_RS06865"
                     /old_locus_tag="MOB22_06845"
     CDS             4477..4638
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MOB22_RS06865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MOB22_06845"
                     /product="hypothetical protein"
                     /protein_id="WP_268314897.1"
                     /transl_table=11
                     /translation="MTIKKKVVLSLILLGLIGGGITSVSSFQSKDLAIDRNTTFLHIHH
                     KDNQKYFA"
     gene            5001..5357
                     /locus_tag="MOB22_RS06870"
                     /old_locus_tag="MOB22_06850"
     CDS             5001..5357
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="MOB22_RS06870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06850"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_268314896.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.7e-09,
                     bitscore: 32.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKVFVTFALGVGLLVPFAAFASNSDEITHGEVDLDKTRNSMTI
                     FKTEKVGGGTWKHGFSKQDNVYSHYNHKKKTHKASVSVGQTLTEGPWKKKNEGYSKASR
                     PAGLFGNKAYWNTK"
     misc_feature    5024..5039
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 21.07"
                     /tool="antismash"
     gene            5473..7659
                     /locus_tag="MOB22_RS06875"
                     /old_locus_tag="MOB22_06855"
     CDS             5473..7659
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="MOB22_RS06875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06855"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_268314894.1"
                     /transl_table=11
                     /translation="MKKMICVLFFLFFISLNILSYNFIVNKQIAYTLFSNKQVLIINYN
                     KSNFNSEIFLKKLVDFSKKEKVNISQYNFLDEKTLNIYTSNIKDEPSLHIRDWKQPSKE
                     RYLSNFEKISEKNVMGSIKFPSSTWKIKYLDINQVNNVGIGNRFYISTTDNIITERAIR
                     QFSNFGDVSLESNNTIDSYMFNTTLALLVLLSIIILFIGILYFAIKNRKQLILHKLWGY
                     SKIKSLFFLPNLFLKPFVIIILFVALLIVILTFTFNLNDWIIIYAKLYIINIIIAILLL
                     TGYKIMITTVLYNNSDISGATKGSMPFKKFQMLSLGFKLVITVLLFNVIALSFTNLFDL
                     KQQLKNKSYWNKTQTIYKLNVTDTGIDYSNLKNDREKNDKLNKLYHELESHKNAFIMYA
                     DNYSVLERKGGKPIYFYTLNSTKENEVYSPSGKNVTISPNYLKINPIIGIKGKQITKKQ
                     LNLDPNTLNLLVPKKYAKFKEKIIKAYKEQFYFQKVEVENMYNKEIGHTSNALKKDRLK
                     INIIYTENSQKYFTFNSELGDINKKNNIVDPIATIFTGNVDTSYIGAYATTSLFFYDDS
                     EGMAYQKLVPYLEKTHTKNLINASVSVYKELSEQIARIQNMFIQNLIGSVIAIILSMAF
                     LVSYIWTYYSVNAYSLYLKEIFGYSYWKRNKHLIIFSLTSNFLLGAGVSLYYNLSLLII
                     FIFLFIGLELIVLYIMSRYLNRKNMNKILKGDRI"
     gene            7656..8276
                     /locus_tag="MOB22_RS06880"
                     /old_locus_tag="MOB22_06860"
     CDS             7656..8276
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328395.1"
                     /locus_tag="MOB22_RS06880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06860"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_268314892.1"
                     /transl_table=11
                     /translation="MIQLENVYKSFDERSILSNFSIKIRENEFVSIIGKSGSGKTTVLN
                     IMSLLDTPDKGEVQILGCKNPNPKEVMKLRRENLGYIFQNYVLMDNETVLTNLSLSTAY
                     AKEFKKDMLPKALEMVGLDKSFLKKKVYQLSGGEQQRVAIARIILKPCDIIFADEPTGN
                     LDEYNKNIILSLFHQLKDMGKTIVCVTHDQEIARCSERVINLS"
     gene            8293..8472
                     /locus_tag="MOB22_RS06885"
                     /old_locus_tag="MOB22_06865"
     CDS             8293..8472
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037932.1"
                     /locus_tag="MOB22_RS06885"
                     /note="Built to rescue LMOF2365_14255 during structural
                     annotation change regression.; Built to rescue
                     LMOF2365_14255 during structural annotation change
                     regression; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06865"
                     /product="bacteriocin-like WGxF protein"
                     /protein_id="WP_268314890.1"
                     /transl_table=11
                     /translation="MNHFIFALYTSILLIIAGIVHRIIFRVFNLPFDSTILFWGGFVVI
                     YFILALVTSLLFRK"
     gene            8727..8905
                     /locus_tag="MOB22_RS06890"
                     /old_locus_tag="MOB22_06870"
                     /pseudo=""
     CDS             8727..8905
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390517.1"
                     /locus_tag="MOB22_RS06890"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06870"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKVIIIEGPQADKCINDCYHYLIKLYRKEIQGDSNICKGINRGTS
                     DQGIEHRQPDRGLY"
     gene            8863..>9031
                     /locus_tag="MOB22_RS06895"
                     /old_locus_tag="MOB22_06875"
                     /pseudo=""
     CDS             8863..>9031
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390454.2"
                     /locus_tag="MOB22_RS06895"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="MOB22_06875"
                     /product="recombinase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKGSSIDSQIEACINRCVEACYEGVSSASFYNVRLWIA"
     gene            complement(9161..9889)
                     /gene="sigK"
                     /locus_tag="MOB22_RS06900"
                     /old_locus_tag="MOB22_06880"
     CDS             complement(9161..9889)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /gene="sigK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003237137.1"
                     /locus_tag="MOB22_RS06900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB22_06880"
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="WP_003237137.1"
                     /transl_table=11
                     /translation="MVTGVFAAFGFVVKELVFLVSYVKNNAFPQPLSSSEEKKYLELMA
                     KGDEHARNMLIEHNLRLVAHIVKKFENTGEDAEDLISIGTIGLIKGIESYSAGKGTKLA
                     TYAARCIENEILMHLRALKKTKKDVSLHDPIGQDKEGNEISLIDVLKSENEDVIDTIQL
                     NMELEKVKQYIDILDDREKEVIVGRFGLDLKKEKTQREIAKELGISRSYVSRIEKRALM
                     KMFHEFYRAEKEKRKKAKGK"
ORIGIN
        1 ccttcaacaa taacagatcg attacaaaca attgagaacg aaaaagaaca gagtgagaat
       61 aacgcagaca aagctgctgg ctaagccagt ggctttttta ttttgcagac agaaggagag
      121 aggatagatg gccatcaaag ttgttaagaa tctagtctct aaatcgaaat atggattgaa
      181 atgtcctaat cctatgaaag ctgaatatat cactattcac aacactgcga atgatgcttc
      241 agcagccaat gagatttctt acatgaagaa taactctagc tcaacgagtt ttcactttgc
      301 agtagacgat aaacaagtca ttcaagggat tccaacgaat cgtaacgctt ggcacacagg
      361 agatggcaca aacggtacag gtaatcgtaa gtcgatcggt gtcgaaattt gttatagcaa
      421 gtcaggagga cctaaataca gggctgctga aaagttggct atcaagtttg tagctcagct
      481 tcttaaagaa cgcggatggg gcattgaacg tgtgcggaag caccaagatt ggagcggaaa
      541 gtattgcccg caccgtattt tagacgaggg acgttggagt gaagttaaag cggcgattga
      601 tgctgaatta aaagcaattg gcggcaaatc atccagcaag aaaacaactt cgcccaaggc
      661 agtgaagaaa tcaagctcaa gcaaaaagaa aacatctttt aatctgcctt ccggcatctt
      721 taaagtgaaa agcccattga tgcatagcga tgctgttaaa cagatccaaa cagccctggc
      781 ggcattgcat ttctatccgg ataagaaagc aaaaaacttc ggtatcgata gctattatgg
      841 accgaaaaca gccgatgccg taagacggtt ccagctgatg aatggactaa atcctgatgg
      901 catttatgga ccgaaaacga aagcaaaaat taaagggctt ttgaagtaat aaaaggtccc
      961 ttgaattttc aagggacctt ttatggatta taaagtttca atattctttt ctattttttg
     1021 aataacttgt tcgagccata ctaatggaga tactccgtaa ctacgaatgt ctactccgtc
     1081 agcagcttca tcgataaaat cattcttttc ttcatccgta taatcactgt taataaaatc
     1141 ttttaatgct actatatcag tttcttgctc ttcttttgga atctcttcta agtattcgtc
     1201 cagtgctgat tctggagttt ctatatcttg atgaaaaata cctcctagaa attgaaaaac
     1261 aggttcggaa atgtttgtat ctatcatggt atcacctcgt tgggtagcca gttaatataa
     1321 agctaccatc tttgttcttt ttcaatataa ttttagcatt agttaaatca gtaattgtat
     1381 cttctccctt tggtattcct cttcctaaaa cttcattccc tttataagag agtactaaag
     1441 gctttttcac atcactattt aaccactttt ctattttcgc tttattatta gtgagagcac
     1501 tattagcaac tctttctgca gttgctctat ctttaaatga tgatgaaccg gatatcttct
     1561 tgttactcat tttgctctta tcaagtcttt gaattagttc ttcgtcggtc ttcccaacat
     1621 gtctttctat aagatgcccg ccttttgcct catgagcagc taatcctcca ccaggtgcga
     1681 gaggggtact atcaccttgt ttgacattac ctatacccat gttgttagaa gtctcagatt
     1741 ttctaagtac actctctttt ttatcttcaa taatactttt tagtagagga gtatttttgg
     1801 cgttatacgt attatcgatg tcttgggcaa tcccgacaag tgcaagatca ttcttatttc
     1861 taacaataga attgatgtaa gctttgcctt tgtttatccc tttattgaca tgagcagcag
     1921 ctgttccacc ttcaaccact ttacctactt tagaaagctt ggtagtggtt tgcactgcgg
     1981 cacccgcacc ttttggccca acgatatttc ctatcgcata actgatataa tgcccttttt
     2041 catatgcatc gccatttacc attttatcat tccatgagtc gaccaggttt gtccagatga
     2101 tttttgaata agaggggacg cttaatactg ttgttattgt ttttgtaagt tttaattcgg
     2161 taggaagctg tgaatattcc caggcgccca caaccaagtc tttgacgcca tatagcgtat
     2221 cgtcaatcgc atcgttaagt ccatcattaa ctccatcgat gaaatcaatt gttgccttac
     2281 cggcattgat cacactttca ccaacttctt cgagctgttt tgcttgttca agctgcacca
     2341 catattgtaa ctgtgcaggc tcaaggttct catacccgat ctccttggca atttctaagt
     2401 attcatccgg atccgtcact ccgtcagcta gcttcttctt cagctctttg atttctcgct
     2461 ctttagcttc ttctttcttg atcgtcaaat aagcttccgt tcgcttctca atatcgcctt
     2521 ttttcttatg tatatcactc tcacgatatg ccttagcgtt gtagtggatg ggcgtagcgt
     2581 ttttgccttt gcccgttgct tcttggagct tttggaaatc tgacttgatg aattgttcat
     2641 ttggttctga tagggcatat tctgtcagta aatcttcgtc aaggtcacct agtttttcaa
     2701 ttgttttttt gcgtttgtca ttggcatccg caagctcatc cttgaaggtt tctgttgaaa
     2761 acaggtcgag cgggaggatg tcgtcaatat cgtttaggat atctttcatg gcctttttct
     2821 gttcagacat gatggacttt gattttttat gggcgttagc cagttcatgt tctaaaaaag
     2881 actcttcaat ataagcatca gagagacctt tgtcctctac agctccggca atgctattaa
     2941 aaaatgcgat tttcatatca atgtagtcga tccattgatc agtgacacca acatgatcgt
     3001 gatagaatgc tttaatgtta ttagcacctt tacctgagaa ttcgctgtcg tccagttcag
     3061 caacgctttg taaagctttt ctcaagttga ccatttgcgt ccttagttct ttatattctt
     3121 tggcgcggtc tgtagcttca gacataagtg ttttagcttc aaagactttc ataatcatac
     3181 cctctcattt aatcatataa caaaatatta ccatggaaga aagaagaaac gaacttgaaa
     3241 ttatcttgat taaccatatc aaaggtaccg aataaaaagt ttgcggtttt tttgcggttc
     3301 aaacactttt tggaaatgtt agaatattac ataaaaagac ataaaaggag gggtcctgtg
     3361 aattcggttt accttcctca catgtagggg ttaagattaa cgaatggtat aagatgattc
     3421 ggcaattcag tatttcagat gcagagatat taaaagaaga agtgacacag aaaattgagc
     3481 gtatggaaga ggatcaagat ttacttatat attttcaatt aatgtgtttt cgacatcaac
     3541 ttatgcttga ttatttagag ccaaagaaca agtatgggga tcgaccaaca ataaaagaac
     3601 ttcttgagaa aattgaagct cctcagaaga agcttacagg ccttttgaag tattattcct
     3661 tgttttttcg tggtatgtat gaatttgatc aaaaagagta tgttgaggca atcaattact
     3721 atcgtaaagc tgaaaaagag ctggggtatg tcactgatga aattgaaaaa gcagaatttc
     3781 actttaaagt ggctgaagct tattacataa tgaaacaaac tcatgtatca atgcatcata
     3841 ttttgcaagc tctagctgtt tatgacactc atactcttta ccaaattcgg aaaattcaga
     3901 gcctgtttgt tatagccggt aattacgatg atttcaaatg ttacgacaag gctattcctc
     3961 atttaaaaaa tgcccttcaa atagctgaca aaatttataa taagcgcatg atgattattg
     4021 ctaacctaaa tattggcctt agttatgata gggcaggaaa ttatatagag gctgctttat
     4081 attttaaaga agcacttaga ttaatcgggg aaaaagaaag tgatttgttc acaaaagttt
     4141 acttcggatt atgttgtgcc ttgtttaaat ctggtcatat aatggatgga gtacctgtat
     4201 tagaaaaagg catagagtac cttgataaaa atccaaacga gttgtatcgt caattatttg
     4261 atttcttgga tgctttgtat gttcaaggta tttacgaaac aaaaattctc gaggtcttag
     4321 agtacctgga ctctaaaaaa ctctatgctt acattgaaga atgtgtcaag tgcgtggctg
     4381 ttgagtatga aaaagcaaat gatcacttga aagcttccag gtactttaaa gcagttttag
     4441 agacgcaaac taaaattcag agaggagatt gtctttatga ctattaagaa gaaagtcgtt
     4501 ctttctctaa ttttattagg gttaataggt ggtggaatta ctagtgtctc aagttttcag
     4561 tctaaagatc tagcgatcga tcgaaacact acattcctgc atatccatca taaagataat
     4621 caaaaatatt ttgcataatt tgcccttgaa aagggctttt ttggttttga gaggtttgag
     4681 tctcgctgct gaaatacctg aagagcaata ttaaaacctc cgtacaagct aagagatttc
     4741 aacagctgta tatacaaagt tatggtttga aaactaaaga gttaatatta tgccataaca
     4801 tagcagtaat taaaggtgga acgtaagtta aatagcccta tcgagatgag attggctggt
     4861 tcaaaatctc cttaaaagtt ttaacgaaag tgtataatgt cataagtata tatgcaaaat
     4921 tatacatata tgctataatc ttgatatata gggtatgttt tacataaccc ttatgaaatt
     4981 ttttcaaagg agagattacg ttgaagaaaa aggtttttgt tacattcgcg ttgggggtag
     5041 gtttactggt tccatttgca gcatttgcta gcaactctga tgaaattact catggggagg
     5101 tagacttgga taaaacaaga aattctatga ctatctttaa aactgaaaaa gtgggtggtg
     5161 ggacttggaa gcatgggttt tctaaacagg ataatgttta ttctcattat aatcacaaaa
     5221 agaagactca caaagctagt gtttcagtag gacaaacatt aactgaggga ccttggaaga
     5281 aaaaaaatga gggttactca aaagcatcta gacctgcggg gctatttgga aacaaagctt
     5341 attggaatac aaaataaaag cgattccata aatataatat aagtttctct tgaatccggt
     5401 gtctataaat tcggaacgtg tattctataa ctcctataga agagacactg gattttttat
     5461 ggaggtttac atatgaagaa aatgatctgt gtattattct ttctgttttt tatctcactg
     5521 aatatattaa gttataactt tattgtcaat aaacaaatag cgtatacttt gttctctaat
     5581 aaacaagttt taattattaa ttataataaa tctaacttta atagtgagat atttctgaag
     5641 aagcttgttg atttttctaa gaaagaaaaa gttaatattt cgcagtataa ttttttggat
     5701 gagaaaacat taaatattta tacttcgaat ataaaagatg agccttcctt acatattagg
     5761 gattggaaac agccatcgaa ggaacgatat ttatctaatt ttgaaaaaat aagcgaaaaa
     5821 aatgtaatgg gctcaattaa attcccatca tcaacatgga aaataaagta tcttgatata
     5881 aatcaagtaa ataacgtggg aattgggaat cggttttaca tttcaactac tgacaatata
     5941 ataacagaaa gggcaattcg tcaattttca aactttggtg atgtgagtct tgaaagtaat
     6001 aacactattg attcgtatat gtttaatact acattagctc tgttagtttt gctttctatt
     6061 ataatcttat ttattggtat cctttatttt gcaattaaaa atcgaaaaca actaatttta
     6121 cataaattat ggggatattc aaaaatcaaa tcgcttttct tcttaccaaa tttatttcta
     6181 aaaccgttcg ttattataat attatttgtt gcccttttaa ttgttatact tacatttact
     6241 tttaatctta atgattggat tatcatatat gctaaactgt atataataaa tattataatt
     6301 gcgattttat tattgacggg gtacaaaata atgataacca ctgttttata taataacagt
     6361 gatataagtg gcgctactaa aggtagtatg ccatttaaaa aatttcaaat gttatcccta
     6421 ggatttaagt tagtcataac agttttatta tttaacgtta ttgccctatc attcacgaac
     6481 ttatttgatt taaaacaaca attaaagaat aaatcatact ggaataagac tcagacaatt
     6541 tacaaactca acgttactga tacaggaatt gactatagta atcttaaaaa cgatagagaa
     6601 aaaaatgata aactgaataa gctctatcat gagcttgaat ctcacaaaaa tgcatttata
     6661 atgtatgctg acaattattc agtattagaa aggaaagggg gaaagccgat ttatttttat
     6721 acactcaatt cgactaaaga aaatgaggta tattctccct ctggaaaaaa tgtaacaata
     6781 agtcctaatt atttaaaaat aaacccgatt atcggcataa aaggaaaaca gatcacaaaa
     6841 aaacaattaa accttgaccc aaatacgttg aatctgcttg ttcctaaaaa atatgcaaag
     6901 tttaaagaaa aaatcatcaa agcttataag gaacagtttt attttcaaaa agttgaagtg
     6961 gagaatatgt ataacaaaga aataggccat acttcaaatg ctctcaaaaa agaccgtttg
     7021 aaaataaata taatatatac tgaaaatagt caaaagtatt tcacctttaa tagcgaactt
     7081 ggtgatataa ataaaaaaaa caacattgtt gatcctattg ccactatatt cactggtaat
     7141 gttgacactt cttacatagg agcatatgca actacatcat tattttttta tgatgattct
     7201 gaaggtatgg cttatcaaaa attagttcct tatttagaga aaacgcatac aaaaaacttg
     7261 attaacgcgt cagtatctgt atataaagaa ttaagtgaac aaattgcaag aattcagaat
     7321 atgtttattc aaaatttaat tggttctgtg atagcaatta ttttatcaat ggccttttta
     7381 gtttcatata tatggactta ctatagtgtg aatgcttata gtctatattt aaaagagata
     7441 tttggatatt catattggaa aagaaataag catttaataa ttttttcact gacttctaat
     7501 tttttgctag gagcaggagt aagtctttat tacaatttaa gtttattaat tattttcatt
     7561 tttttgttta taggattaga attaattgtt ctatatatta tgagtagata tttgaataga
     7621 aaaaacatga ataaaatatt aaaaggggat agaatatgat ccaacttgaa aacgtatata
     7681 aaagttttga tgaaagatca attctttcta atttttccat taaaataaga gaaaatgaat
     7741 ttgtttctat tattggaaag agtggttcag gaaaaaccac tgttttaaat ataatgagct
     7801 tattggatac tccggataag ggagaagtcc aaatattagg atgtaaaaat cctaacccaa
     7861 aagaagtcat gaaattaagg agagagaact taggttacat ttttcaaaac tatgtgctta
     7921 tggacaatga aacagtgtta acaaacctgt cactttccac tgcttatgcc aaagaattta
     7981 aaaaagatat gttaccaaaa gctttagaga tggtcggttt agataaaagc tttttaaaga
     8041 aaaaagtcta tcagttaagt ggtggagagc aacaacgcgt cgctatagct agaataattt
     8101 tgaaaccttg tgatattatt tttgctgatg aaccaacagg aaatttagat gaatataata
     8161 agaacattat tttatcttta tttcatcagt tgaaagacat ggggaaaact atcgtatgtg
     8221 taacgcatga tcaagaaata gctagatgtt ctgaaagagt aattaatctt tcataagggg
     8281 agttgaaaca aaatgaatca ttttattttt gcactgtata ctagcatcct tttaattatt
     8341 gcaggtatcg ttcatagaat aatatttaga gtgtttaatc taccgtttga tagtacaatt
     8401 ttgttctggg gagggtttgt tgttatttat tttatattag ccttagtgac ttccttatta
     8461 ttcaggaaat aacatagtaa tctcagaaca tttgcaagac ctagattata taactaaggc
     8521 atgagactct tttgaactct agcttttgta aatatacaaa ccgcttgttt ctatgaagct
     8581 gttgagtttg tgtttctcct gtaaaacacc agtgtttaat acaatggtgt tttttctctt
     8641 tctttacaac ccatattcac attataaaat aaactcatta ggacaaacca cctttacata
     8701 gcattaccca aggggtgatg cattttatga aggtgataat catcgaggga ccgcaagctg
     8761 acaaatgcat taacgattgc tatcattatt taataaaact ttataggaag gagatccagg
     8821 gtgatagcaa tatatgtaag ggtatcaacc gaggaacaag cgatcaaggg atcgagcatc
     8881 gacagccaga tcgaggcctg tataaatagg tgtgttgaag catgctatga aggggtttct
     8941 agcgcgagct tttataacgt ccggctttgg attgcttgag agaggatgca agtaagcaag
     9001 taaagggctt ataagtaaag tcatctgctt ctcggggagc ttatcagcga tgacaaaaaa
     9061 tgattttcat aactctttca tatattcttt ctctattttt ttaaaaagaa actttaatga
     9121 aatagagaaa gccggaatgt tagatccggc ctctgctaaa tcatttcccc ttcgcctttt
     9181 tccgcttttc cttctccgcc cgataaaact catgaaacat cttcatcagc gcccgttttt
     9241 caatccgcga tacatagctg cgcgagatac cgagttcttt ggcaatctca cgctgcgttt
     9301 tttccttctt caaatcaagc ccaaaccgcc cgacaatcac ttccttctca cggtcatcta
     9361 aaatatcaat gtattgcttc actttttcaa gctccatatt gagctggatc gtatcaatca
     9421 cgtcttcatt ttctgatttt agtacatcga tcaggcttat ttcattccct tccttatcct
     9481 gcccgatcgg atcatgaagg gaaacgtctt tttttgtttt tttcaatgcg cgtaaatgca
     9541 tgagaatctc attttcaata caccgcgcag catacgtcgc cagctttgtc ccttttccgg
     9601 cggaatagct ttcaattcct ttgatcagcc cgattgttcc gatggagatt aagtcctctg
     9661 catcctcacc tgtattttcg aactttttca caatatgggc gaccaagcga agattatgct
     9721 caatcagcat gtttctggca tgttcatctc ctttagccat gagctctaag tatttttttt
     9781 cctcgctgct tgagagcggt tgtggaaagg cattgttttt cacgtaagat actaaaaaga
     9841 caagctcttt aacaacaaag ccgaacgctg cgaaaacacc tgtcaccatc gtcacctcca
     9901 caaaagtatg taggcaaaag cctatatgta aatgtatgtg agcgggaggc tgtctgtgtc
     9961 tgtaccggga aatttctcgc aggggagcct ggctttcgtg agcagcaggc caacctcaaa
    10021 ttttaaaaat aatctttatc atgcgcccat acattgaaat gaacgatgta tggggggatg
    10081 ggaatgaaaa aatggatggc aggcctgctt cttgctgcag cagtttttct ttgtttagtg
    10141 gttccgcagc cgatcaagag cgcttctttg tatgacaaag tgttgtattt tccgctgtct
    10201 cgttatccgg aaacagggga tcatattaga gatgcgatag cagagggaca ttctgatatt
    10261 tgtaccatcg acagggatgg agcggacaaa aggcgagagg aatcattaaa aggaattccg
    10321 acgaagccgg gctatgaccg ggatgagtgg ccaatgg
//
