LOCUS       NZ_JALAKZ010000024      8744 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Bacillus inaquosorum strain N9C1 contig_0000024, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAKZ010000024
VERSION     NZ_JALAKZ010000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 455.1098425649713x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 03:19:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,217
            Genes (coding)                    :: 4,075
            CDSs (with protein)               :: 4,075
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 86 of 142
            Pseudo Genes (incomplete)         :: 82 of 142
            Pseudo Genes (internal stop)      :: 25 of 142
            Pseudo Genes (multiple problems)  :: 41 of 142
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:33:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8744
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAKZ010000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..3428)
                     /locus_tag="MOB76_RS21410"
                     /old_locus_tag="MOB76_21360"
                     /pseudo=""
     CDS             complement(<1..3428)
                     /NRPS_PKS="Domain: Condensation_Starter (3-300). E-value:
                     2.7e-89. Score: 291.4. Matches aSDomain:
                     nrpspksdomains_MOB76_RS21410_Condensation_Starter.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOB76_RS21410"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="MOB76_21360"
                     /product="amino acid adenylation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1e-75, bitscore:
                     242.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-15, bitscore:
                     45.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQHTYSLTHAQRRVWFTELLEPDTSICNLTACVKFRGNIDLDVL
                     EGALNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQA
                     RIPFKLFNSPLYQFYLLRIHNHEVLLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDP
                     LPDQPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSAS
                     RDTITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKA
                     EKEMLGMFVSSLPIRITVDPDADFLSFVRRVGREQLSVMRHQRFPYNLLVNELRKEQKD
                     LHNLIGISMQYQPLQWHNADGFDYETALYFSGYTATELSVQIQERTDTGTIQLNFDYQN
                     TLFSLEDIKRIQNHLLTLLENALQHPHSFIKELDMTNIREKQKLLYEFNKTEAVSPEAP
                     TLHGLFERQAAVTPERPAIRFSSDSLTYAELDMYASRLAARLAARGVTKESIVGVLSER
                     SPEMLIAVLAVLKAGGAYXAERQRGRALAGTVGMFRTELFRRDA"
     source          1..8744
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="N9C1"
                     /submitter_seqid="contig_0000024"
     region          1..8744
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..8744
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8744
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3428](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3428)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(1773..1820)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB76_RS21410_0006"
                     /evalue="4.50E-01"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOB76_RS21410"
                     /protein_end="552"
                     /protein_start="536"
                     /score="3.2"
                     /tool="antismash"
                     /translation="AERQRGRALAGTVGMF"
     CDS_motif       complement(2376..2489)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB76_RS21410_0005"
                     /evalue="1.00E-09"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="MOB76_RS21410"
                     /protein_end="351"
                     /protein_start="313"
                     /score="30.9"
                     /tool="antismash"
                     /translation="REQLSVMRHQRFPYNLLVNELRKEQKDLHNLIGISMQY"
     aSDomain        complement(2529..3419)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB76_RS21410_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="2.70E-89"
                     /label="MOB76_RS21410_Condensation_Starter.1"
                     /locus_tag="MOB76_RS21410"
                     /protein_end="300"
                     /protein_start="3"
                     /score="291.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="HTYSLTHAQRRVWFTELLEPDTSICNLTACVKFRGNIDLDVLEGA
                     LNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQARIP
                     FKLFNSPLYQFYLLRIHNHEVLLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDPLPD
                     QPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSASRDT
                     ITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKAEKE
                     MLGMFVSSLPIRITVD"
     CDS_motif       complement(2541..2630)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB76_RS21410_0004"
                     /evalue="6.70E-09"
                     /label="C5_starter_265-294"
                     /locus_tag="MOB76_RS21410"
                     /protein_end="296"
                     /protein_start="266"
                     /score="28.2"
                     /tool="antismash"
                     /translation="DLAIGTYYGNRGSKAEKEMLGMFVSSLPIR"
     CDS_motif       complement(2964..3029)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB76_RS21410_0003"
                     /evalue="5.30E-08"
                     /label="C3_starter_134-155"
                     /locus_tag="MOB76_RS21410"
                     /protein_end="155"
                     /protein_start="133"
                     /score="25.7"
                     /tool="antismash"
                     /translation="FHHIIMDGISLNVMGNQIIDLY"
     CDS_motif       complement(3234..3350)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB76_RS21410_0002"
                     /evalue="3.60E-10"
                     /label="C2_starter_024-063"
                     /locus_tag="MOB76_RS21410"
                     /protein_end="65"
                     /protein_start="26"
                     /score="32.5"
                     /tool="antismash"
                     /translation="ICNLTACVKFRGNIDLDVLEGALNHSISRNDAIRFQLLE"
     CDS_motif       complement(3375..3413)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB76_RS21410_0001"
                     /evalue="3.00E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="MOB76_RS21410"
                     /protein_end="18"
                     /protein_start="5"
                     /score="10.5"
                     /tool="antismash"
                     /translation="YSLTHAQRRVWFT"
     misc_feature    complement(3468..3484)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 22.38"
                     /tool="antismash"
     gene            complement(3799..5277)
                     /gene="dacB"
                     /locus_tag="MOB76_RS21415"
                     /old_locus_tag="MOB76_21365"
     CDS             complement(3799..5277)
                     /EC_number="3.4.16.4"
                     /GO_function="GO:0004175 - endopeptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241941.1"
                     /locus_tag="MOB76_RS21415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB76_21365"
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="WP_268292946.1"
                     /transl_table=11
                     /translation="MKKSIKLYVAVLLLFVVASVPYMHQATQAAEKQGALAGQIDKILA
                     DHPALEGAMAGITVRSAETGAVLYEHSGEIRMRPASSLKLLTAASSLSVLGEDYSFTTE
                     VRTDGKFKGKKLNGNLYLIGKGDPTLLPSDFEEMAEKLKQSGVKEIKGNLIGDDTWYDD
                     IRLSPDMPWSDEYTYYGAQISALTASPNEDYDAGTVIVEITPGKKEGEKPAVSVSPKTD
                     YVTIKNDAETTAAGQKKDLTIEREHGTNTITIEGSVPVDASKTKEWIAVWEPAGYALDL
                     FKQALKKQEITVKGHVKTGTAPGSSDVLISHRSMPLSKLFVPFMKLSNNGHAEVLVKEM
                     GKVKKEEGSWEKGLEVLNNALPEFGVDPKSLVLRDGSGVSHINAVSSDQISQLLYDIQD
                     EKWFSAYLNSLPVAGNSDRMIGGTLRNRMKDTPAQGKVRAKTGSLSTVSSLSGYAETKS
                     GKTLVFSILLNGLIDDEDGKDIEDQIAVILANQS"
     gene            complement(5312..6289)
                     /gene="galM"
                     /locus_tag="MOB76_RS21420"
                     /old_locus_tag="MOB76_21370"
     CDS             complement(5312..6289)
                     /codon_start=1
                     /gene="galM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389718.1"
                     /locus_tag="MOB76_RS21420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB76_21370"
                     /product="aldose 1-epimerase"
                     /protein_id="WP_225516923.1"
                     /transl_table=11
                     /translation="MANDIEKITYLGTPAIKAGNEHIEMIVVPEWGSNVISLVDKTTQL
                     QLLREPETAESFHDTPTLYGIPILFPPNRISDGTFSFRGRTYHFDINEKDKHNHLHGFL
                     YQEKWNVVTMKQTDEEVIVETEIDLSELPHVYKQFPHHAVVRMTYTLKGNTLFKHATVM
                     NKGKEAFPWGIGYHTTFVFPEESSLFSLTADQQWELDERLLPTGKLMDVQNKEALHEGM
                     DLRSQQLDDVFLSSYQKRGGENQAVIYHQHAHISIIYKADEQFKHWVVYNADGKQGYLC
                     PEPYTWVTNAVNLNVSPSLTGVQVLEPGEETTAKSSITIELNHQ"
     gene            complement(6422..7813)
                     /locus_tag="MOB76_RS21425"
                     /old_locus_tag="MOB76_21375"
     CDS             complement(6422..7813)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389719.2"
                     /locus_tag="MOB76_RS21425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB76_21375"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_003241939.1"
                     /transl_table=11
                     /translation="METSQEQLVTQKKSNPSVWRSMSLFLVPLLLSNVLQSVGQLVGMM
                     AVGRWLGVDAVAAVSSFFPLFFLLISFTIGIGSGSSILIGQAYGAKNEERLKAVVGTTL
                     TFTFLLGVVLAVIGSIFTLDILRLMGTPENVIHVSASYARILFYAMPFMFLYFAYTTFL
                     RGTGDSKTPFYTLIVSTVINIALLPVLILGMFGFPKLGIYGSAYATVISTIATFIVLMV
                     YLRKRNHPLQFDKTVRRYLKMDKELLALLLRLGIPSSINMILVSLSEIAVISFVNHYGS
                     NATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANEFGRLKQVIRVGIWLNYIIGG
                     VLIILIYVFSHQILGLFLTEQETLYIAHRLLMITLWSYLLFGNAQIISATMRASGTVLW
                     PTVISIFAIWGVEVPVAFVLSHYTNLEILGVWVGYPAAFAVSLLLIYGYYQFVWKKKQI
                     TRLIQ"
     gene            8100..8645
                     /gene="iseA"
                     /locus_tag="MOB76_RS21430"
                     /old_locus_tag="MOB76_21380"
     CDS             8100..8645
                     /codon_start=1
                     /gene="iseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389720.2"
                     /locus_tag="MOB76_RS21430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB76_21380"
                     /product="DL-endopeptidase inhibitor IseA"
                     /protein_id="WP_003241937.1"
                     /transl_table=11
                     /translation="MKKCFLFLTTIALILSLSTNAFAKNTSGDLSQKQALQLALSAREH
                     FWNTMSGHNPKAKKAVCPSGTFEYQNLQYVYMCSDLGTKAKAVNYLTPIFTKTAIEKGF
                     KDYHFTVSKGKLAVPIGDGDNLLNWKKSTAKLISKKGGTVTYEFTVPTLDGSPAAKRKV
                     TFVKENKKWKVNQFDAVI"
ORIGIN
        1 gcggcgcttg gctgagatca aagggctcga taaacgcggc tgcggcttcc tgttcggttt
       61 gttcgctgag gactgctgtc tgtaatgtaa acggcaccac gttatggatg cgctgcaccg
      121 gctcgccgct ttcgtcctgt tcgaaggatg tccgaagcga ctcgtggcgc ttgatcagct
      181 ctttaaaagc cctgtccagt ctttcggggt ccagcttccc ttccagctct aacacagccg
      241 gcatattgta gcctgtccct ccgtcctcca gctgctgaag gacatagatc cgtttttgcg
      301 cggaggaaac cggataggcc tcacgctgtt ccgccggttt catcgcttca tatggactgt
      361 ccgttccttc acggataacg gaagccagtc cttcaacagt cggatgggca aacacatctt
      421 tcagcggcac ctgtacatcg aattctttgg caatccgtga cacaagcgcc gttgctttta
      481 aggaatgtcc tccgcggtcg aaaaagttgt cgtgaatccc gacaggacca tttttcagca
      541 cgtcctccca cagctgggca agcttcatct ctgtcacatt tctcggcgcc gtgtaggtct
      601 ccgcatctgc agctccacca ggagctggca gggcgtttcg gtccagcttt ccgcttggcg
      661 taaccggcca ccgttccatc tctatcatat gagctgggat catgtagctc ggcagcagtc
      721 tttcaagctg cgtccgcact tcgtttcttc ttagtccttc tgtataggcg cataattccg
      781 gctcgccgct gtctgtccgt acggtaacgg ctgcctctct gacgccttca atgcttctaa
      841 gcgctgcttc aatttctcca ggctcgatac ggtagcccct gattttgact tgatcatcca
      901 aacggccgag gaattcaaca tgtccgtcag gaagccagcg cgccaaatcc ccagttttgt
      961 acatgcgttc acccgggtaa aacgggtcct caagaaaacg ctcctccgtt aaggccggcc
     1021 gattcaaata gcctctggcc acacccgctc cggctatgta cagttcgccg gcgacaccgc
     1081 aaggctgtac ggctaaatgc gcatctaaaa catacaaacg cgcaccgggt acgggcttcc
     1141 cgatcggaat ccgcaagcga tccctgtccc gctctgggtc caacacgtaa aatgccgcgt
     1201 caaccgttgc ttctgtcggc ccgtagccat gaatcagcga tacctgcggc aataaagaag
     1261 caaaacgggc tgccacatgg ggcgcaagcg gttcacctcc ggcgaatata cgcttcaggc
     1321 ttgtcccgtc actcagcctt tcgatttccg cttgatcgag aaagctgttc agcatagccg
     1381 gaataaaatg ggccgttgta atactttcct gatgaatggc ttgtacgatc aatgccgggt
     1441 ctttttccca tccgggcgga agcagataag ccgaagcgcc ggaaagggac caccaaaaca
     1501 gctgccacac agacgcatcg aaggaaaaag aggttttcac cataacaatg tcatcttcct
     1561 gaagcggaaa ctgacgctgc atgccagtca gaaaggaaac agcctggcgg tgctcgacgg
     1621 ctaccccttt tggcttcccg gtggagcccg atgtatagat gacataggcg agagaacagc
     1681 cgtcagcaga agtaaacaca tcgttttctg ctttttcgct cgcaagagat gccatgtcca
     1741 cttcaagcat ctctccggaa aagctcggtg cggaacatcc cgactgtgcc agcaagagcg
     1801 cggccccgct gtctttcagc nnaatacgcc ccgcctgcct tcaatacagc aagtaccgct
     1861 atcagcattt caggtgaccg ttcagacagg acaccgacga tgctttcctt cgtgacgccg
     1921 cgtgccgcga ggcgcgcagc cagccggctg gcatacatgt caagctcagc gtacgtcagc
     1981 gaatcgctgg aaaagcggat ggctggccgc tccggtgtga cagccgcctg tcgttcaaac
     2041 agaccgtgca gggttggggc ttcaggcgaa acagcttccg tcttattaaa ctcatacaac
     2101 aatttttgtt tctctcttat attagtcata tctaattctt ttatgaaact gtgcggatgt
     2161 tgaagggcgt tttccagtaa cgtaagcaga tggttctgta ttcgcttaat atcctcaaga
     2221 gaaaacaagg tattttgata gtcgaaattt aattgaatag ttccggtgtc tgtccgttcc
     2281 tgtatttgaa cagaaagctc ggtcgcggtg tatccgctga aataaagcgc cgtttcatag
     2341 tcaaaaccgt ctgcattgtg ccattggagc ggttgatact gcatagatat accgataagg
     2401 ttgtgtaaat ccttctgttc ttttcttaat tcatttacca gcaaattata aggaaaccgt
     2461 tggtgacgca taacggaaag ctgttccctg cctactctgc ggacaaagga tagaaagtcc
     2521 gcgtcaggat caaccgttat tctgatggga agagaactga cgaacatgcc gagcatttct
     2581 ttttcagcct ttgaccctct gttgccatag taggtgccga tggcaaggtc ttttttagaa
     2641 gtgatccggc tgatgcatat gtaaaatgaa gccataaata aggaaatgat attgatcttt
     2701 tgttcttcgc aaaagattcg aatcgtttgc cctagatgag gtgaaagggt gatggtatct
     2761 ctggatgcgg atgtgctctg tttttgtaaa gacgtctggc cggctaacga gtggtatccc
     2821 ggcggattct gaaaggtttg agtccaaaat aaacggtcct ttgtaaaacg tgaagactgt
     2881 aaatattgac tctctttttc gatgtagctc aagtaagaag gttcaggctg atcgggtaaa
     2941 ggatcttgct ttttcatttt ttgataaaga tctataattt gatttcccat cacgtttaag
     3001 gagatgccgt ccattataat atgatggaat ttagcaaaca gcaaaacctc atgattgtgg
     3061 attctaagga gataaaattg ataaagaggc gagttaaata gtttaaatgg gattctcgct
     3121 tgttcttgta tccattgctc tatttctatc atttcaacat ttgaaaaatc tattattccc
     3181 aatgaagtat atttatattc agtaaggtac aatcgcggct caagctcttc tccttctagc
     3241 agctgaaacc tgatggcatc gttgcgggag atggaatgat tcagcgcgcc ctctagaaca
     3301 tcaagatcaa tattgcctct gaatttcaca caggccgtaa gattgcaaat gctggtatct
     3361 ggctccagca gttcggtaaa ccacactctc ctttgggcat gggttaaaga ataagtatgt
     3421 tggctcaacg gattccctcc agttctcata ataagagagt aagaaaaaag tagaaaaatg
     3481 acaataaaag aaattatatg actatataat ccatatatgc actatattaa cggatatttt
     3541 ttaaagtaca ataaaaaata ttaaattttt ataacatttt tttgaatata gtattatttc
     3601 tttagttttg gtttctttct tcatcaaatt tttgaacata aagtgataaa agtatcattc
     3661 tgattcgggt cttacacact aatttttcaa ttgtgctaat ttactgttgc gcgtaatctt
     3721 tctatttgtt ggcagatgcg accggaatat tattgaagac ataaaaaaac ctgttttcct
     3781 tggaaaacag gtctgacgct atgattgatt tgccaagatg acagcgattt gatcctcgat
     3841 atcttttcca tcctcgtcat caatcaatcc gttcaaaaga atagaaaaaa caagggtttt
     3901 tccgctcttg gtttctgcgt acccggataa agagctgact gtgcttaacg atccggtttt
     3961 tgcccttacc tttccttgtg caggagtgtc tttcatccgg ttcctcagtg ttccgcctat
     4021 catcctgtca ctattgcccg caaccggcag agaatttaga taagctgaga accatttctc
     4081 atcttgaatg tcatataaca gctgtgagat ttgatctgag gatacagcat taatatggga
     4141 gacacctgat ccgtctctta agacaagtga tttgggatca acaccaaatt ccggaagcgc
     4201 attgtttaat acttcaagtc ccttttccca gcttccttct tcttttttca ctttccccat
     4261 ctcttttacg agcacttcgg catgtccgtt attgcttaat ttcataaacg gcacaaatag
     4321 ttttgacaga ggcattgagc gatgagagat taacacgtct gaagaaccgg gcgcagtgcc
     4381 tgtcttgacg tgccctttta cagtaatttc ttgttttttt agtgcctgct taaataaatc
     4441 caaagcgtat ccagccggct cccagacagc aatccattcc tttgtcttgc ttgcgtcaac
     4501 cggtacgctc ccttcaattg tgatcgtgtt tgttccatgc tctctttcga tcgtgaggtc
     4561 tttcttttgg ccagccgcag tcgtttcggc atcgttctta atcgttacat aatccgtctt
     4621 cggagataca gaaacggcag gcttttcccc ctctttttta cccggggtga tctctacaat
     4681 cacagtgcct gcatcataat cttcattcgg agacgctgtt aaagcggaga tttgcgcgcc
     4741 gtaatatgtg tattcatcac tccacggcat atcaggcgat aatcggatgt catcatacca
     4801 cgtatcatca ccgatcagat tgcctttgat ctctttcacg ccggattgtt tcaatttctc
     4861 agccatttct tcaaaatcag atggtaaaag tgtcggatct cctttcccta tcaaataaag
     4921 gtttccattc agctttttcc ctttaaattt gccgtccgtc cggacctcgg ttgtaaagga
     4981 atagtcctcg ccaagaacag aaagtgatga ggccgctgtc aaaagtttta aagaagaggc
     5041 gggcctcatt cggatctctc ctgaatgctc atatagaaca gcgcctgttt ccgcagaacg
     5101 aacggtgatg ccggccattg cgccttcgag tgcaggatga tcagctaaaa tcttatcgat
     5161 ctgcccggca agtgcacctt gtttttcagc cgcttgcgta gcctgatgca tataaggtac
     5221 cgaagcaacg acgaaaagca gcaaaacagc aacataaagc tttatgcttt ttttcatttt
     5281 tttaccccgc ctctctgcgc ctcatcatgg gctattgatg attcagttca atggtgatac
     5341 tgcttttggc tgtcgtttct tctcccggct ccagcacttg tacaccagtg agtgatggag
     5401 acacattaag attcacagcg tttgtcaccc atgtgtaagg ttcagggcat aaatatccct
     5461 gctttccgtc cgcgttgtat acaacccaat gtttaaattg ttcatctgcc ttataaataa
     5521 tgcttatatg tgcatgctga tgataaatga ctgcctgatt ttctccgccc cttttctgat
     5581 aagaagataa aaagacatca tccagctgct ggctgcgtaa atccatccct tcatgcaagg
     5641 cttctttatt ctgtacatcc atcagtttcc cggtcggcag caggcgctca tccaactccc
     5701 attgctgatc tgctgtcagg gagaataacg agctttcttc tggaaataca aatgtagtat
     5761 gatagccgat tccccaagga aacgcttcct ttcctttgtt catgaccgtc gcatgtttaa
     5821 atagagtatt cccctttagc gtgtatgtca tccgaacgac ggcatgatgc ggaaattgct
     5881 tatacacatg gggaagctct gaaaggtcaa tttcagtttc aacgatcact tcttcatcag
     5941 tttgcttcat ggtcactacg ttccattttt cctgataaag aaacccatga agatggttgt
     6001 gtttatcctt ttcattgata tcaaaatgat atgttctgcc ccggaaactg aatgtaccgt
     6061 cacttatccg gttaggcgga aacaggatcg gaatgccata caatgttggt gtatcatgga
     6121 agctttctgc tgtttccggt tcacgcaaaa gctgaagctg tgtcgttttg tccacaagag
     6181 aaatgacgtt gctcccccac tcgggaacga caatcatttc tatatgttcg ttgcctgctt
     6241 tgatggcagg tgttcctaaa tacgtaatct tctcaatatc gtttgccatg tgtactcaac
     6301 ctctccttga ttcattgctt gtataagaat tcaatgtaat gtgccatatt tcctccattt
     6361 atgttttctc tttatcaaac aaaaaacacc gttcaaatgt tgaacggtgt gattcgagat
     6421 gctattggat gagacgtgta atctgtttct ttttccaaac gaattgataa tacccataaa
     6481 tcaacagcaa gctgacggca aacgcagcag gatagccaac ccagacgccg agtatttcaa
     6541 gatttgtgta gtgggaaagt acgaacgcga caggcacctc tactccccag atggcaaaaa
     6601 tactgataac ggtcggccat aaaaccgttc cgcttgcccg catcgtcgca gaaataattt
     6661 gggcatttcc aaagagtaag taactccaca atgtaatcat caagagacgg tgcgcaatgt
     6721 aaagcgtctc ttgttcggtt aaaaataatc ctaaaatctg atgtgaaaaa acatagatca
     6781 gaatgatcag cacaccgcca atgatataat tcagccaaat cccgacacga attacctgtt
     6841 ttaggcggcc aaactcgttc gctccgatcg attgcgcggc gaatatagac accgcaatac
     6901 caaggctgac ggccggcatc tgcacatagc tggctacctg gttgaccacg ccgtatgcag
     6961 ctgtcgcatt agagccataa tggttgacga atgaaataac cgctatctct gataatgaga
     7021 cgagaatcat attgatgctt gatggaatcc cgaggcgcag caggagagcc agaagttctt
     7081 tatccatttt caaataacgg cgcactgttt tatcaaattg cagcggatgg tttcgttttc
     7141 gcaaatacac cattaacaca ataaaagtcg caatcgtgga aatgacggtt gcatatgcgg
     7201 agccgtatat gcccagtttc ggaaaaccga acatgccaag gatcaagaca ggcaaaagcg
     7261 ctatgttaat gaccgtgctc acaatcagcg tataaaaagg tgtcttagaa tccccagttc
     7321 cgcgcaaaaa tgttgtataa gcaaaatata aaaacataaa cggcatcgcg taaaataaaa
     7381 tacgtgcata gctggcgctg acatgaatta cattttcagg cgtgcccatt aaacgaagaa
     7441 tatccaaggt gaaaatactg ccgatgacgg ctaacacaac ccctaataaa aacgtaaacg
     7501 tgagcgtcgt cccgacaaca gcctttaacc gctcctcatt tttcgccccg taggcttgtc
     7561 cgatcaggat cgagcttcct gaaccgatcc caattgtaaa tgagattaaa agaaaaaata
     7621 acgggaaaaa ggatgataca gctgcaaccg catcaacacc aagccatctg cctacagcca
     7681 tcatcccgac taactggccg actgattgca gtacattgct tagcagaagc ggaactaaaa
     7741 agagtgacat agaccgccag acagatggat tgcttttctt ctgagttaca agttgttctt
     7801 gtgatgtttc catattgaaa gctcctttat ttgaattcgt ttgtaaacat gaccctattc
     7861 ttttgacaaa aacaggatat gtaacatgtt attattcatc actgtacata tgaaaccctt
     7921 cccgattctt ctaattttac acatcttttt agcttatgaa gaataaacta gcaagtaaca
     7981 gccttttttc ttgtgagtaa aagaataaaa aataatttat gcaaaagtat tgtaatctat
     8041 ccgtaattat tgtaacattt gtaacataag agaacgagat tttgaaggag tgaaccacaa
     8101 tgaaaaaatg ttttctattt ctaacaacga ttgcacttat tctgtcatta agcacaaatg
     8161 catttgcgaa aaatacttca ggtgatttat cacaaaaaca agcactgcag ctggcgttat
     8221 cagcaagaga gcatttttgg aacacaatga gcggccacaa tccaaaagcg aaaaaagcag
     8281 tttgcccatc aggcactttt gagtatcaaa atcttcaata cgtgtacatg tgcagtgacc
     8341 taggcactaa agcaaaagca gtgaattact taactcctat tttcacaaaa acagcaatcg
     8401 aaaaaggctt taaagactat cacttcactg tttcaaaagg aaaactagcc gtgccaatcg
     8461 gcgacggtga caacctttta aattggaaaa aatcaactgc taaactgatc tctaaaaaag
     8521 gcggcacagt cacatacgaa tttaccgttc ctactttaga cggatcacct gcagcgaaac
     8581 ggaaagtgac atttgtaaaa gaaaataaaa aatggaaagt caatcaattt gacgcagtta
     8641 tataagaaaa aacgccctgc ataagggact gaccccataa gatgagacaa ataaaaacac
     8701 cttcaagttt gaatacggat gattgttatc cgaaataaga cttg
//
