LOCUS       NZ_JALAJC010000030      1275 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Bacillus inaquosorum strain S8C1X1 contig_0000030, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAJC010000030
VERSION     NZ_JALAJC010000030.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 228.72242007437796x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 03:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,337
            Genes (coding)                    :: 4,218
            CDSs (with protein)               :: 4,218
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 82 of 119
            Pseudo Genes (incomplete)         :: 62 of 119
            Pseudo Genes (internal stop)      :: 20 of 119
            Pseudo Genes (multiple problems)  :: 35 of 119
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:37:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1275
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAJC010000030.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1275
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="S8C1X1"
                     /submitter_seqid="contig_0000030"
     gene            complement(<1..>1275)
                     /locus_tag="MOB18_RS22065"
                     /old_locus_tag="MOB18_22015"
     region          1..1275
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1275
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1275
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1275](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1275)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..1275)
                     /NRPS_PKS="Domain: PCP (299-367). E-value: 6.7e-28. Score:
                     88.7. Matches aSDomain: nrpspksdomains_MOB18_RS22065_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388231.2"
                     /locus_tag="MOB18_RS22065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_22015"
                     /product="AMP-binding protein"
                     /protein_id="WP_268353874.1"
                     /sec_met_domain="AMP-binding (E-value: 9.7e-63, bitscore:
                     198.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.5e-21, bitscore:
                     61.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2e-14, bitscore:
                     39.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIWEMFPTWTIGAELHVIDESIRLDIVRLNDYFETHGVTITFLP
                     TQLAEQFMELENTSLRVLLTGGDKLKRAVKQPYTLVNNYGPTENTVVATSTEINPEEGS
                     LSIGQAIANTRVYILGDGNQVQPEGVAGELCVAGRGLARGYLNREDETAKRFVADPFVP
                     GERMYRTGDLVKWTGGGIEYIGRIDQQVKVRGYRIELSEIEVQLAQLSEVQDAAVTAVK
                     DKGGHTAIAAYVTPETADIEALKSSLKETLPDYMIPAFWVTLNELPVTANGKVDRKALP
                     EPDIEAGSGEYKAPTTDMEELLAGIWQDVLGISEIGVSDNFFSLGGDSIKGIQMASRLN
                     QHGWKLEMKDLFQHPTIEELTQYVERAEGKQADQGPVEGEVILTPIQRWFFEKNFTNKH
                     HWNQSVMLHAKKGFDPERVEKTLQAL"
     CDS_motif       complement(100..129)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS22065_0005"
                     /evalue="2.40E-05"
                     /label="NRPS-E1"
                     /locus_tag="MOB18_RS22065"
                     /protein_end="392"
                     /protein_start="382"
                     /score="16.9"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     aSDomain        complement(175..378)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS22065_PCP.1"
                     /evalue="6.70E-28"
                     /label="MOB18_RS22065_PCP.1"
                     /locus_tag="MOB18_RS22065"
                     /protein_end="367"
                     /protein_start="299"
                     /score="88.7"
                     /tool="antismash"
                     /translation="EELLAGIWQDVLGISEIGVSDNFFSLGGDSIKGIQMASRLNQHGW
                     KLEMKDLFQHPTIEELTQYVERA"
     CDS_motif       complement(286..315)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS22065_0004"
                     /evalue="4.40E-03"
                     /label="PCP_mC"
                     /locus_tag="MOB18_RS22065"
                     /protein_end="330"
                     /protein_start="320"
                     /score="10.4"
                     /tool="antismash"
                     /translation="NFFSLGGDSI"
     CDS_motif       complement(664..729)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS22065_0003"
                     /evalue="7.60E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOB18_RS22065"
                     /protein_end="204"
                     /protein_start="182"
                     /score="37.1"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
     CDS_motif       complement(808..897)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS22065_0002"
                     /evalue="1.50E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="MOB18_RS22065"
                     /protein_end="156"
                     /protein_start="126"
                     /score="52.4"
                     /tool="antismash"
                     /translation="PEGVAGELCVAGRGLARGYLNREDETAKRF"
     CDS_motif       complement(1000..1029)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS22065_0001"
                     /evalue="2.00E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="MOB18_RS22065"
                     /protein_end="92"
                     /protein_start="82"
                     /score="10.7"
                     /tool="antismash"
                     /translation="NYGPTENTVV"
ORIGIN
        1 cagcgcctgc aacgttttct ccacacgttc aggatcaaag cccttcttgg catgaagcat
       61 caccgattgg ttccagtggt gcttgttcgt gaagttcttc tcgaagaacc agcgctgaat
      121 cggtgtcagg atgacttcgc cctccactgg gccttggtcc gcctgtttgc cttcggcacg
      181 ttccacgtat tgggtgagct cttcgatcgt cgggtgctgg aagagatctt tcatttccag
      241 cttccagccg tgctggttca agcggctcgc catttggatt cctttgatgg aatctccgcc
      301 gagcgagaag aaattgtcac tgacaccgat ctccgaaatg ccgagcacat cctgccagat
      361 gcctgcaagc agctcttcca tatccgttgt cggcgctttg tattctccgc ttcccgcttc
      421 gatgtccggc tccggcaacg ctttgcggtc aacctttccg tttgcggtaa ctggaagctc
      481 gttcagcgtc acccagaacg ccgggatcat atagtccggc agggtttctt tcaacgaaga
      541 ctttaatgct tctatgtcag ctgtttccgg tgtgacatac gccgcgatcg ctgtatggcc
      601 gcctttatct ttgacagctg tcaccgccgc atcctgtacc tcagaaagct gggcgagctg
      661 gacttcgatt tctgagagct cgatccgata gccgcggacc ttgacctgct ggtcgatccg
      721 gccgatgtat tcaatgccgc cgcccgtcca tttcaccaaa tcgccggtgc ggtacatgcg
      781 ttctcccggc acaaacggat cagcgacaaa ccgcttcgcg gtttcgtctt ctcgattcaa
      841 atagccgcgc gccaggccgc gtcccgccac gcaaagctct ccggctacgc cttccggctg
      901 cacctgattg ccatcgccga gaatgtacac tctcgtattg gcgatcgcct gcccgatgga
      961 aagcgagcct tcctccggat tgatttctgt gctcgtggca acgaccgtat tttctgtcgg
     1021 cccgtagttg ttgacgagcg tgtacggctg tttgaccgcg cgtttcagct tgtctcctcc
     1081 agtcaagagg acacggagtg atgtgttctc aagctccatg aactgttctg ccagctgtgt
     1141 cggcaggaac gtgatcgtta cgccgtgcgt ttcgaaatag tcgttcaagc ggacgatatc
     1201 gaggcggatc gattcatcaa tgacgtgaag ctcggcgccg attgtccagg tcgggaacat
     1261 ctcccaaatg gaggc
//
