LOCUS       NZ_JALAJC010000025     10368 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Bacillus inaquosorum strain S8C1X1 contig_0000025, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAJC010000025
VERSION     NZ_JALAJC010000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 228.72242007437796x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 03:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,337
            Genes (coding)                    :: 4,218
            CDSs (with protein)               :: 4,218
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 82 of 119
            Pseudo Genes (incomplete)         :: 62 of 119
            Pseudo Genes (internal stop)      :: 20 of 119
            Pseudo Genes (multiple problems)  :: 35 of 119
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:37:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10368
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAJC010000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..1337
                     /locus_tag="MOB18_RS21795"
                     /old_locus_tag="MOB18_21750"
     source          1..10368
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="S8C1X1"
                     /submitter_seqid="contig_0000025"
     region          1..10368
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10368
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10368
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1373:5201](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1374..5201
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        <7..851
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21795_Epimerization.1"
                     /evalue="1.10E-143"
                     /label="MOB18_RS21795_Epimerization.1"
                     /locus_tag="MOB18_RS21795"
                     /protein_end="282"
                     /protein_start="0"
                     /score="471.7"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="HWNQSVMLHAKKGFDPERVEKTLQALIEHHDALRMVYREENGNIL
                     QAYKSISESKVSFEIVDLYGSDENMLKSQIKILANRLQSSLDLQNGPLLKAEQYRTEAG
                     DHLLIAVHHLVVDGVSWRILLEDFASGYMQAEKEGNIVFPQKTNSFKDWAEELAAFSQS
                     AHLLQQAEYWAQIDAEQVSPVPKDYETVQRMVKHTSSVLCELTEEDTKHLLTDVHQPYG
                     TEINDILLSALGLTMKEWTDGTKIGINLEGHGREDIIPNVNISRTVGWFTAQYPVVLDM
                     S"
     aSModule        <7..1307
                     /domains="nrpspksdomains_MOB18_RS21795_Epimerization.1"
                     /domains="nrpspksdomains_MOB18_RS21795_TIGR01720.1"
                     /incomplete
                     /locus_tags="MOB18_RS21795"
                     /tool="antismash"
                     /type="unknown"
     CDS             <5..1337
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (0-282). E-value:
                     1.1e-143. Score: 471.7. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21795_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (285-434). E-value: 1.5e-54.
                     Score: 176.0. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21795_TIGR01720.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388231.2"
                     /locus_tag="MOB18_RS21795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21750"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268353839.1"
                     /sec_met_domain="Condensation (E-value: 4.1e-78, bitscore:
                     251.5, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HWNQSVMLHAKKGFDPERVEKTLQALIEHHDALRMVYREENGNIL
                     QAYKSISESKVSFEIVDLYGSDENMLKSQIKILANRLQSSLDLQNGPLLKAEQYRTEAG
                     DHLLIAVHHLVVDGVSWRILLEDFASGYMQAEKEGNIVFPQKTNSFKDWAEELAAFSQS
                     AHLLQQAEYWAQIDAEQVSPVPKDYETVQRMVKHTSSVLCELTEEDTKHLLTDVHQPYG
                     TEINDILLSALGLTMKEWTDGTKIGINLEGHGREDIIPNVNISRTVGWFTAQYPVVLDM
                     SEADASAVIKSVKENLRRIPDKGVGYGILRYVTETADTKGFTPEISFNYLGQFDSEVKT
                     DFFEPSAFDMGRQVSEESEALYALSFSGMIRNGRFVLSCSYNEKEFERATVEEQMERFK
                     ENLLMLIRHCTEKEDKEFTPSDFSAEDLEMDEMGDIFDMLEENLK"
     CDS_motif       45..119
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21795_0001"
                     /evalue="1.50E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOB18_RS21795"
                     /protein_end="38"
                     /protein_start="13"
                     /score="20.6"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       324..389
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21795_0002"
                     /evalue="2.80E-12"
                     /label="NRPS-E2"
                     /locus_tag="MOB18_RS21795"
                     /protein_end="128"
                     /protein_start="106"
                     /score="38.7"
                     /tool="antismash"
                     /translation="LLIAVHHLVVDGVSWRILLEDF"
     CDS_motif       792..842
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21795_0003"
                     /evalue="1.10E-08"
                     /label="NRPS-E5"
                     /locus_tag="MOB18_RS21795"
                     /protein_end="279"
                     /protein_start="262"
                     /score="27.1"
                     /tool="antismash"
                     /translation="NISRTVGWFTAQYPVVL"
     aSDomain        861..1307
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21795_TIGR01720.1"
                     /evalue="1.50E-54"
                     /label="MOB18_RS21795_TIGR01720.1"
                     /locus_tag="MOB18_RS21795"
                     /protein_end="434"
                     /protein_start="285"
                     /score="176.0"
                     /tool="antismash"
                     /translation="ASAVIKSVKENLRRIPDKGVGYGILRYVTETADTKGFTPEISFNY
                     LGQFDSEVKTDFFEPSAFDMGRQVSEESEALYALSFSGMIRNGRFVLSCSYNEKEFERA
                     TVEEQMERFKENLLMLIRHCTEKEDKEFTPSDFSAEDLEMDEMGD"
     CDS_motif       882..941
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21795_0004"
                     /evalue="6.70E-12"
                     /label="NRPS-E6"
                     /locus_tag="MOB18_RS21795"
                     /protein_end="312"
                     /protein_start="292"
                     /score="37.4"
                     /tool="antismash"
                     /translation="VKENLRRIPDKGVGYGILRY"
     CDS_motif       975..1004
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21795_0005"
                     /evalue="1.10E-03"
                     /label="NRPS-E7"
                     /locus_tag="MOB18_RS21795"
                     /protein_end="333"
                     /protein_start="323"
                     /score="11.3"
                     /tool="antismash"
                     /translation="PEISFNYLGQ"
     aSDomain        1374..1469
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21800_NRPS-COM_Nterm.1"
                     /evalue="1.60E-15"
                     /label="MOB18_RS21800_NRPS-COM_Nterm.1"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="32"
                     /protein_start="0"
                     /score="48.0"
                     /tool="antismash"
                     /translation="MSQFSKDQVQDMYYLSPMQEGMMFHTILNPGQ"
     gene            1374..5201
                     /gene="srfAC"
                     /locus_tag="MOB18_RS21800"
                     /old_locus_tag="MOB18_21755"
     CDS             1374..5201
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-32). E-value: 1.6e-15.
                     Score: 48.0. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21800_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (9-308). E-value:
                     1.6e-117. Score: 383.8. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21800_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (469-867). E-value:
                     1.6e-111. Score: 364.8. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21800_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (973-1042). E-value: 9.2e-25. Score:
                     78.6. Matches aSDomain: nrpspksdomains_MOB18_RS21800_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (1060-1255). E-value:
                     8.7e-36. Score: 116.4. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21800_Thioesterase.1"
                     /codon_start=1
                     /gene="srfAC"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388233.2"
                     /locus_tag="MOB18_RS21800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21755"
                     /product="surfactin non-ribosomal peptide synthetase SrfAC"
                     /protein_id="WP_268353840.1"
                     /sec_met_domain="Condensation (E-value: 2.2e-94, bitscore:
                     304.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.1e-122, bitscore:
                     397.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 9e-18, bitscore:
                     53.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Thioesterase (E-value: 1.9e-49, bitscore:
                     157.9, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQFSKDQVQDMYYLSPMQEGMMFHTILNPGQSFYLEQITMKVKG
                     SLNIKCLEESMNVIMDRYDVFRTVFIHEKVKRPVQVVLKKRQFHIEEIDLTHLTGSEQT
                     AKINEYKEQDKIKGFDLTRDIPMRAVIFKKADESFEWVWSYHHIILDGWCFGIVVQDLF
                     KVYNALREQKPYSLPPVKPYKDYIKWLEKQDKQASLRYWREYLEDFEGQTTFAEQRKKQ
                     KGQYEPKELLFSLPKAETKAFTELAKSQHTTLSTALQAVWSVLISRYQQSGDLTFGTVV
                     SGRPAEIKNVEHMVGLFINVVPRRVKLSEDITFNGLLKQLQEQSLQSEPHQYVPLYDIQ
                     SQADQPKLIDHIIVFENYPLQDAKNEENSENGFDMEDVHVFEKSNYDLNLMASPGDEML
                     IKLAYNENVFDEAFILRLKSQLLTAIQQLIEKPDHPVNTINLVDDKEREFLLTGLNPPA
                     QTHEAKPLTEWFKEAVNVNPDAPALKYSGQTLSYRELDEEANRLARRLQKEGAGKGSVV
                     ALYTKRSLELVIGILGVLKAGAAYLPIDPKLPEDRISYMLTDSSAACLLTHQEMKEKAA
                     QLPYTGTTLFIDDQTRFAEQACDLEISIEPDDPAYIMYTSGTTGKPKGNITTHANIQGL
                     VKHVDYMAFSEKDTFLSVSNYAFDAFTFDFYASMLNAARLIIADEHTLLDTERLTDLIH
                     QENVNVMFATTALFNLLTDAGEDWMKGLRCVLFGGERASVPHVRKALRVMGPGKLINCY
                     GPTEGTVFATAHIVHDIPDAISSLPIGKPISNASVYILNEQNQLQPFGAVGELCISGMG
                     VSKGYVNRHDLTKQKFITNPFKPGETLYRTGDLARWLPDGTIEYAGRIDDQVKISGHRI
                     ELEEIEKQLQEYPGVKDAVVVADRHESGDASINAYLVNRTQLSAADIKTHLKKQLPAYM
                     VPQTFTFMDELPLTTNGKVNKRLLPKPDQDQLAEEWIGPRDETEETIAQIWSEILGRQQ
                     VGIHDDFFALGGHSLKAMTAASRIKKELAIDLPVKLLFEAPTIAGISAYLKNGGSDGLQ
                     DVTIMNPDQDQIIFAFPPVLGYGLMYQNLASRLPSYKLCAFDFIEEENRLDRYADLIQK
                     LQPEGSLTLFGYSAGCSLAFEAAKKLEEQGRIVQRIIMVDSYKKQGVSDLDGRTVESDV
                     EALMNVNRDNEALNSEAVKQGLKQKTHAFYSYYVNLISTGQVKADIDLLTSGVDFDMPE
                     WLASWEEATTGAYRVKRGFGTHAEMLQGETLDRNAGILLEFLNTQTVTVS"
     aSDomain        1401..2297
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21800_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="1.60E-117"
                     /label="MOB18_RS21800_Condensation_DCL.1"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="308"
                     /protein_start="9"
                     /score="383.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIILDG"
                     /tool="antismash"
                     /translation="QDMYYLSPMQEGMMFHTILNPGQSFYLEQITMKVKGSLNIKCLEE
                     SMNVIMDRYDVFRTVFIHEKVKRPVQVVLKKRQFHIEEIDLTHLTGSEQTAKINEYKEQ
                     DKIKGFDLTRDIPMRAVIFKKADESFEWVWSYHHIILDGWCFGIVVQDLFKVYNALREQ
                     KPYSLPPVKPYKDYIKWLEKQDKQASLRYWREYLEDFEGQTTFAEQRKKQKGQYEPKEL
                     LFSLPKAETKAFTELAKSQHTTLSTALQAVWSVLISRYQQSGDLTFGTVVSGRPAEIKN
                     VEHMVGLFINVVPRRVKL"
     aSModule        1401..5138
                     /complete
                     /domains="nrpspksdomains_MOB18_RS21800_Condensation_DCL.1"
                     /domains="nrpspksdomains_MOB18_RS21800_AMP-binding.1"
                     /domains="nrpspksdomains_MOB18_RS21800_PCP.1"
                     /domains="nrpspksdomains_MOB18_RS21800_Thioesterase.1"
                     /final_module
                     /locus_tags="MOB18_RS21800"
                     /monomer_pairings="Leu -> Leu"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1410..1451
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0001"
                     /evalue="8.00E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="26"
                     /protein_start="12"
                     /score="14.6"
                     /tool="antismash"
                     /translation="YYLSPMQEGMMFHT"
     CDS_motif       1473..1589
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0002"
                     /evalue="2.50E-12"
                     /label="C2_DCL_024-062"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="72"
                     /protein_start="33"
                     /score="39.1"
                     /tool="antismash"
                     /translation="FYLEQITMKVKGSLNIKCLEESMNVIMDRYDVFRTVFIH"
     CDS_motif       1806..1871
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0003"
                     /evalue="1.70E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="166"
                     /protein_start="144"
                     /score="36.5"
                     /tool="antismash"
                     /translation="YHHIILDGWCFGIVVQDLFKVY"
     CDS_motif       1917..1955
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0004"
                     /evalue="5.50E-04"
                     /label="C4_DCL_171-183"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="194"
                     /protein_start="181"
                     /score="12.4"
                     /tool="antismash"
                     /translation="PYKDYIKWLEKQD"
     CDS_motif       2193..2288
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0005"
                     /evalue="7.60E-18"
                     /label="C5_DCL_263-294"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="305"
                     /protein_start="273"
                     /score="56.9"
                     /tool="antismash"
                     /translation="DLTFGTVVSGRPAEIKNVEHMVGLFINVVPRR"
     CDS_motif       2337..2450
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0006"
                     /evalue="1.00E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="359"
                     /protein_start="321"
                     /score="56.6"
                     /tool="antismash"
                     /translation="QEQSLQSEPHQYVPLYDIQSQADQPKLIDHIIVFENYP"
     aSDomain        2781..3974
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21800_AMP-binding.1"
                     /evalue="1.60E-111"
                     /label="MOB18_RS21800_AMP-binding.1"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="867"
                     /protein_start="469"
                     /score="364.8"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FKEAVNVNPDAPALKYSGQTLSYRELDEEANRLARRLQKEGAGKG
                     SVVALYTKRSLELVIGILGVLKAGAAYLPIDPKLPEDRISYMLTDSSAACLLTHQEMKE
                     KAAQLPYTGTTLFIDDQTRFAEQACDLEISIEPDDPAYIMYTSGTTGKPKGNITTHANI
                     QGLVKHVDYMAFSEKDTFLSVSNYAFDAFTFDFYASMLNAARLIIADEHTLLDTERLTD
                     LIHQENVNVMFATTALFNLLTDAGEDWMKGLRCVLFGGERASVPHVRKALRVMGPGKLI
                     NCYGPTEGTVFATAHIVHDIPDAISSLPIGKPISNASVYILNEQNQLQPFGAVGELCIS
                     GMGVSKGYVNRHDLTKQKFITNPFKPGETLYRTGDLARWLPDGTIEYAGRIDDQVKIS"
     CDS_motif       2967..3008
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0007"
                     /evalue="1.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="545"
                     /protein_start="531"
                     /score="18.3"
                     /tool="antismash"
                     /translation="LGVLKAGAAYLPID"
     CDS_motif       3201..3260
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0008"
                     /evalue="9.20E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="629"
                     /protein_start="609"
                     /score="24.4"
                     /tool="antismash"
                     /translation="AYIMYTSGTTGKPKGNITTH"
     CDS_motif       3768..3857
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0009"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="828"
                     /protein_start="798"
                     /score="39.8"
                     /tool="antismash"
                     /translation="PFGAVGELCISGMGVSKGYVNRHDLTKQKF"
     CDS_motif       3939..4004
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0010"
                     /evalue="9.50E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="877"
                     /protein_start="855"
                     /score="33.7"
                     /tool="antismash"
                     /translation="YAGRIDDQVKISGHRIELEEIE"
     aSDomain        4293..4499
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21800_PCP.1"
                     /evalue="9.20E-25"
                     /label="MOB18_RS21800_PCP.1"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="1042"
                     /protein_start="973"
                     /score="78.6"
                     /tool="antismash"
                     /translation="EETIAQIWSEILGRQQVGIHDDFFALGGHSLKAMTAASRIKKELA
                     IDLPVKLLFEAPTIAGISAYLKNG"
     aSDomain        4554..5138
                     /ASF="active site serine present: True"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21800_Thioesterase.1"
                     /evalue="8.70E-36"
                     /label="MOB18_RS21800_Thioesterase.1"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="1255"
                     /protein_start="1060"
                     /score="116.4"
                     /tool="antismash"
                     /translation="IFAFPPVLGYGLMYQNLASRLPSYKLCAFDFIEEENRLDRYADLI
                     QKLQPEGSLTLFGYSAGCSLAFEAAKKLEEQGRIVQRIIMVDSYKKQGVSDLDGRTVES
                     DVEALMNVNRDNEALNSEAVKQGLKQKTHAFYSYYVNLISTGQVKADIDLLTSGVDFDM
                     PEWLASWEEATTGAYRVKRGFGTHAEMLQGET"
     CDS_motif       4698..4772
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21800_0011"
                     /evalue="1.70E-08"
                     /label="NRPS-te1"
                     /locus_tag="MOB18_RS21800"
                     /protein_end="1133"
                     /protein_start="1108"
                     /score="26.9"
                     /tool="antismash"
                     /translation="QPEGSLTLFGYSAGCSLAFEAAKKL"
     gene            5229..5957
                     /gene="srfAD"
                     /locus_tag="MOB18_RS21805"
                     /old_locus_tag="MOB18_21760"
     CDS             5229..5957
                     /NRPS_PKS="Domain: Thioesterase (14-231). E-value: 1.7e-54.
                     Score: 177.6. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21805_Thioesterase.1"
                     /codon_start=1
                     /gene="srfAD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004: thioesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224939.1"
                     /locus_tag="MOB18_RS21805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21760"
                     /product="surfactin biosynthesis thioesterase SrfAD"
                     /protein_id="WP_003240143.1"
                     /sec_met_domain="Thioesterase (E-value: 3.1e-69, bitscore:
                     222.6, seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.8e-16, bitscore:
                     49.9, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 3.7e-08, bitscore: 22.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQLFKSFDASEKTQLICFPFAGGYSASFRPLHAFLQGECEMLAA
                     EPPGHGTNQTSAIEDLEELTDLYKQELNLRPDRPFVLFGHSMGGMITFRLAQKLEREGI
                     IPQAVIISAIQPPHIQRKKVSHLPDDQFLDHIIQLGGMPAELVENKEVMSFFLPSFRSD
                     YRALEQFELHDVTPIQSPVHVFNGLDDEKCIRDAEGWKKWAKDITFHEFAGGHMFLLSH
                     TEEVAERIFAILNQHPIIQS"
     aSDomain        5271..5921
                     /ASF="active site serine inconclusive"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21805_Thioesterase.1"
                     /evalue="1.70E-54"
                     /label="MOB18_RS21805_Thioesterase.1"
                     /locus_tag="MOB18_RS21805"
                     /protein_end="231"
                     /protein_start="14"
                     /score="177.6"
                     /tool="antismash"
                     /translation="QLICFPFAGGYSASFRPLHAFLQGECEMLAAEPPGHGTNQTSAIE
                     DLEELTDLYKQELNLRPDRPFVLFGHSMGGMITFRLAQKLEREGIIPQAVIISAIQPPH
                     IQRKKVSHLPDDQFLDHIIQLGGMPAELVENKEVMSFFLPSFRSDYRALEQFELHDVTP
                     IQSPVHVFNGLDDEKCIRDAEGWKKWAKDITFHEFAGGHMFLLSHTEEVAERIF"
     CDS_motif       5454..5525
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21805_0001"
                     /evalue="1.90E-08"
                     /label="NRPS-te1"
                     /locus_tag="MOB18_RS21805"
                     /protein_end="99"
                     /protein_start="75"
                     /score="26.7"
                     /tool="antismash"
                     /translation="PDRPFVLFGHSMGGMITFRLAQKL"
     gene            6055..7281
                     /locus_tag="MOB18_RS21810"
                     /old_locus_tag="MOB18_21765"
     CDS             6055..7281
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240144.1"
                     /locus_tag="MOB18_RS21810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21765"
                     /product="MFS transporter"
                     /protein_id="WP_268289412.1"
                     /transl_table=11
                     /translation="MRASPMMKWFVLLYTFVFAIGLNSFRNSFQFFMLPMADTFHADRS
                     LISVSVSIFMITTGIVQFFVGFFIDRFSVRKIMALGAVCISASFLVLPYSPNVHVFSAI
                     YGVLGGIGYSCAVGVTTQYFISRWFDTHKGLALAILTNANSAGLLLLSPIWAAAPYHAG
                     WQNTYTILGIVMAAVLLPLLAFGMKHPPHAQAQPVKKSYDWRGFWNVMKQSRLIHILYF
                     GVFTCGFTMGIIDAHLVPMLKDAHVSHVNGMMAAFGAFIIIGGLLAGWLSDFFGSRSVI
                     LSILFFIRLLSLICLLIPILGIHHSELWYFGFILLFGLSYTGVIPLTAASISESYQTGL
                     IGSLLGINFFIHQIAGALSVYAGGLFYDMTHNYLLIVVVCVVLVGLSALLELVPFKNIH
                     FQHKERIDI"
     gene            complement(7308..7847)
                     /locus_tag="MOB18_RS21815"
                     /old_locus_tag="MOB18_21770"
     CDS             complement(7308..7847)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019257448.1"
                     /locus_tag="MOB18_RS21815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21770"
                     /product="YcxB family protein"
                     /protein_id="WP_041904700.1"
                     /transl_table=11
                     /translation="MIQHSVFLEGAISLRDMRYYFIYRQRKAAVICYIYIFLLSALVYS
                     VYGFPFTVYNNETLELIYNGIFIAVVSLIFTLIFTAFLYLRLRTGYKNSERLKRKRTYT
                     INRQGIQIQSKNYDTLFEWNEIKGAAEYRHLFSIKTSSHQLILIPQRFFKFENERHSFR
                     QLVKEHVPAKKVKLQS"
     gene            complement(7897..8841)
                     /locus_tag="MOB18_RS21820"
                     /old_locus_tag="MOB18_21775"
     CDS             complement(7897..8841)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240146.1"
                     /locus_tag="MOB18_RS21820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21775"
                     /product="DMT family transporter"
                     /protein_id="WP_268289413.1"
                     /transl_table=11
                     /translation="MLKKQQASAYMAAILYSFIIGLSFLFVKIALQTAEPFDILAYRFT
                     IAFVAATVPVLFGWVKLSIRVKDVIAILPLALLYPALFFSFQAFGLVYSSSSEAGIIQA
                     AIPIFTMVFASYVLKERSTWTQKGFTVLSVAGVMFIFVMKGVDVESASLKGSLLILLSA
                     LSSAMYNTAARKMTQRFKLTELTYIMSAIGFVAFNAIALVRHGTAGTASTYFQPFREPG
                     FVLAIVYLGVLSSLVTSFLSNYTLSRIEAFKMSAFNHVSTIVTMIAGVAILDENLAWYH
                     LAGAVCIIIGVLGSNINLQKKKKHQRGTISMNK"
     misc_feature    8828..8837
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    8830..8843
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.54"
                     /tool="antismash"
     gene            8966..10300
                     /locus_tag="MOB18_RS21825"
                     /old_locus_tag="MOB18_21780"
     CDS             8966..10300
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (96-438). E-value:
                     2.3e-24. Score: 78.1. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21825_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388238.1"
                     /locus_tag="MOB18_RS21825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21780"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_268290321.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.9e-25, bitscore:
                     78.2, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKYMSLLTRIEEMMQSSAYQEGDRLPSIRQLSVRYQVSKSTVIR
                     ALQELEKRHLIYSVPKSGYYIVKKTGKAKSGQQGPIDFATSAPDPDVFPYLDFQHCINK
                     AIDTYKNDLFIYGTPKGLPSLIRVLRKLLANQQVFADERHIFITSGVQQALSLLCAMPF
                     PNGKEKIAIEQPGYHLMIEQLETLGIPAIGVKRTEEGLDIAEIERLFQTESIKFFYTMP
                     RFHNPLGCSLSERNKQELVRLAETYDVYLVEDDYLGDLEENKKADPLYAYDLSSHVIYL
                     KSFSKMMFPGLRVGAAVLPDALIDTFHTYKKLNDIDCSMISQAALEIYLKSGMYGRHKE
                     KIRASYKERSLRLHQAIQTHRQLGRGRFTFSSGQAPCMHTHLVLPQDLPASRVIQRLKK
                     QGVLLEAIDRHYLSDYPKENLLKINISNVKTEDIEHGVKLLLSHL"
     aSDomain        9254..10279
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21825_Aminotran_1_2.1"
                     /evalue="2.30E-24"
                     /label="MOB18_RS21825_Aminotran_1_2.1"
                     /locus_tag="MOB18_RS21825"
                     /protein_end="438"
                     /protein_start="96"
                     /score="78.1"
                     /tool="antismash"
                     /translation="DFQHCINKAIDTYKNDLFIYGTPKGLPSLIRVLRKLLANQQVFAD
                     ERHIFITSGVQQALSLLCAMPFPNGKEKIAIEQPGYHLMIEQLETLGIPAIGVKRTEEG
                     LDIAEIERLFQTESIKFFYTMPRFHNPLGCSLSERNKQELVRLAETYDVYLVEDDYLGD
                     LEENKKADPLYAYDLSSHVIYLKSFSKMMFPGLRVGAAVLPDALIDTFHTYKKLNDIDC
                     SMISQAALEIYLKSGMYGRHKEKIRASYKERSLRLHQAIQTHRQLGRGRFTFSSGQAPC
                     MHTHLVLPQDLPASRVIQRLKKQGVLLEAIDRHYLSDYPKENLLKINISNVKTEDIEHG
                     VK"
     CDS             complement(10295..>10364)
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234549.1"
                     /locus_tag="MOB18_RS21830"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21785"
                     /product="4'-phosphopantetheinyl transferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAVCAAHPDFPEDITMVSYEALL"
     gene            complement(10295..>10368)
                     /locus_tag="MOB18_RS21830"
                     /old_locus_tag="MOB18_21785"
                     /pseudo=""
ORIGIN
        1 accactggaa ccaatcggtg atgcttcatg ccaagaaggg ctttgatcct gaacgtgtgg
       61 agaaaacgtt gcaggcgctg atcgagcacc atgatgcact ccgtatggtc tatcgcgagg
      121 aaaacggaaa catccttcaa gcgtataaat ctatcagcga aagcaaagtc agcttcgaaa
      181 tcgtggatct gtatggctcc gatgaaaaca tgctgaaaag ccagatcaag attctcgcga
      241 acaggctgca aagcagtctc gatctgcaaa acggaccgct tctcaaggcg gagcaatacc
      301 gtacggaagc tggcgaccac ctgctcatcg ctgtacacca tctcgtggtc gacggcgtgt
      361 catggcggat tttgcttgaa gattttgctt caggctacat gcaggctgag aaagaaggga
      421 acattgtctt cccgcaaaaa acaaattcct tcaaggattg ggcggaagaa ctggcggcat
      481 tcagtcaatc agcgcatctt ttacagcagg ctgaatactg ggcgcaaatt gacgctgaac
      541 aggtttctcc tgtgccgaag gattatgaaa cagtgcagcg gatggtcaaa catacatcat
      601 ctgtcctctg tgaattaacg gaagaagaca ccaaacatct cttaacggat gttcatcagc
      661 catatggaac tgaaatcaat gatattcttc tcagcgcgct cggtctaaca atgaaagaat
      721 ggacagacgg taccaaaatc ggcattaatc tggagggaca cggacgggag gacatcattc
      781 cgaatgtgaa tatctccaga acggtcggct ggtttacggc acaatatcct gttgtgctcg
      841 acatgtctga agcagatgcc tcagccgtga tcaaatcagt caaagaaaat ctgcgccgca
      901 ttccggataa aggtgttggc tacggcattc tccgttatgt cacagaaaca gcggatacaa
      961 agggcttcac gccagaaatc agctttaact atttgggcca attcgacagt gaagtaaaaa
     1021 ccgacttctt tgagccgtcc gctttcgata tggggcgcca agtaagcgag gaatcagaag
     1081 cgctgtacgc tttaagcttc agcggcatga tcaggaacgg ccgatttgtg ctttcctgct
     1141 cctacaatga gaaggagttt gaaagagcta cggtcgagga gcaaatggaa cggtttaaag
     1201 aaaacctcct gatgctgatc cgccattgta cggaaaaaga ggacaaggaa ttcacgccga
     1261 gcgacttcag cgccgaagac cttgaaatgg acgagatggg cgatatcttt gacatgcttg
     1321 aggagaattt aaaataaaac gcaagggaat tacagaaggc gggagcgaag catatgagtc
     1381 aatttagcaa ggatcaggtt caagatatgt attacctatc gccgatgcag gaagggatga
     1441 tgtttcatac catcctgaat cccggccaga gcttttacct tgaacaaatc acgatgaaag
     1501 taaaaggcag cttgaatatc aaatgccttg aagaaagcat gaatgtgatc atggaccggt
     1561 acgatgtatt tcgtaccgtg ttcattcacg aaaaagtaaa aagacctgtc caagtcgtat
     1621 tgaaaaaaag gcagtttcat atagaagaaa tcgatctgac acacttaacg ggcagcgagc
     1681 aaacagccaa aatcaatgaa tacaaagaac aagataagat caagggattt gatttgacgc
     1741 gggatatccc aatgcgggca gtcattttca aaaaagctga tgaaagcttt gaatgggtgt
     1801 ggagctacca ccacattatt ttggacggat ggtgctttgg catcgtcgtg caggatctgt
     1861 ttaaagtata caatgccctg cgcgaacaaa agccgtacag tctgccccct gtcaaaccgt
     1921 ataaagacta catcaagtgg cttgaaaagc aggataaaca agcatcactg cgttattggc
     1981 gcgagtactt agaggatttt gaaggacaga cgacgtttgc ggagcaaaga aagaaacaga
     2041 agggccaata cgagccgaaa gagctgctct tctcactgcc gaaggcggaa acaaaggcct
     2101 ttaccgagct tgcaaaatcg cagcatacca ctttgagtac ggcgctccag gcagtctgga
     2161 gcgtattgat cagccgctat cagcagtctg gcgatttgac cttcgggaca gtcgtttccg
     2221 ggcgtcctgc ggaaatcaaa aacgttgaac atatggtcgg gctgtttatc aacgttgtcc
     2281 caagacgggt gaaactgtct gaggatatca catttaacgg tttgctcaag cagctgcagg
     2341 agcaatcgct tcagtctgag ccgcatcaat atgtgccgct ctatgacatc caaagccaag
     2401 ccgatcaacc gaaactgatt gaccacatca ttgtctttga gaattatccg cttcaggatg
     2461 caaaaaatga agaaaacagt gaaaacggct ttgatatgga ggatgtacat gtttttgaga
     2521 agtcgaatta tgatctcaat ctaatggctt ccccagggga tgagatgctg attaagcttg
     2581 cctataatga gaatgtgttt gatgaagcgt ttatcctgcg cttgaaatct cagcttctca
     2641 cagcgattca gcagctcatt gagaagccgg atcaccctgt aaacacaatc aacctcgttg
     2701 acgacaagga gagagagttt ttgcttaccg gcttaaaccc gccggctcaa actcatgagg
     2761 caaagcctct gacggaatgg ttcaaggaag cggtgaacgt taatcctgat gcaccggcgc
     2821 tcaagtattc cggacagact ctttcctatc gcgaattaga tgaggaagcg aaccgccttg
     2881 cacgccgttt gcaaaaggaa ggcgcgggca aaggctccgt tgtagctttg tacacgaagc
     2941 gttcgcttga actggtgatc ggcattctcg gtgtattaaa agcgggagcg gcctatctgc
     3001 cgattgatcc gaagctgccg gaggaccgaa tctcgtatat gctgactgac agctccgcag
     3061 cctgtctgct gacacatcag gagatgaaag aaaaagcggc tcagctgccg tatacaggga
     3121 caacgctctt cattgatgat caaacacggt ttgcagaaca ggcatgtgac cttgagatct
     3181 cgattgaacc tgacgatccg gcatatatca tgtacacgtc cggtacaacc ggaaagccga
     3241 aaggcaatat caccactcat gccaatattc aaggattggt caagcatgta gactacatgg
     3301 cattttccga aaaagatacg ttcttatctg tttcgaatta cgcctttgac gcatttacct
     3361 ttgatttcta cgcttcaatg ctgaatgcgg cacggctcat tatcgcagat gaacatacgc
     3421 tgcttgatac agaacgtcta accgatctga ttcatcaaga gaatgtcaat gtcatgtttg
     3481 cgacaactgc actatttaat cttctcacag atgcgggaga ggattggatg aaggggcttc
     3541 gctgcgtgtt attcggcgga gagcgtgcgt ctgtgcctca cgtcagaaaa gcgctgcggg
     3601 tcatggggcc gggcaagctg attaactgct atgggcctac tgagggaacg gtgtttgcga
     3661 cagctcacat cgtgcatgat ataccagatg ccatctcctc attgccgatt ggaaagccga
     3721 ttagcaatgc cagtgtttat attctcaacg agcaaaacca gctccagcca ttcggagcgg
     3781 tcggtgaact gtgcatcagc ggaatgggcg tgtcaaaagg gtatgtaaac cgtcatgacc
     3841 tgacgaagca aaagtttatt acgaacccgt tcaagccggg agaaacgctt taccgcacag
     3901 gggatttagc acgctggctg ccggatggaa caattgaata cgccggccgt attgacgacc
     3961 aggtcaaaat aagcggacac cggattgagc ttgaagaaat cgaaaagcag ctgcaggaat
     4021 atccgggtgt gaaagatgcg gttgttgttg cagaccgcca tgagtctggc gatgcatcaa
     4081 tcaacgccta cctcgtgaac cggacgcagc tttcagctgc agacataaag acacacctga
     4141 aaaaacagct tcctgcttac atggtgccgc aaacctttac tttcatggac gagcttcctt
     4201 taacgacaaa cgggaaggtc aataaacggc tgctgccaaa accggatcag gatcagctgg
     4261 cggaagaatg gatcgggccc cgcgacgaga cggaagaaac gatcgcacaa atctggtccg
     4321 agattctcgg cagacagcaa gtcggcattc atgacgattt ctttgcgctt ggcgggcact
     4381 ccttaaaggc aatgaccgcc gcttcccgca tcaagaaaga gctcgcgatt gatctcccgg
     4441 taaaactttt gtttgaagcg ccgacgatcg ccggcatttc agcgtatctg aaaaacgggg
     4501 gatcagatgg attgcaggat gtaacgataa tgaatcccga tcaggatcag atcattttcg
     4561 cattcccgcc ggtcttgggc tatggcctca tgtaccaaaa cctggccagc cgcctgccgt
     4621 catacaagct gtgcgccttt gatttcattg aggaggaaaa ccggcttgac cgctatgcgg
     4681 atttgattca gaagcttcag ccggaagggt ctttgacact gttcgggtat tcagcggggt
     4741 gcagcctggc gtttgaagct gccaaaaagc ttgaggaaca aggacgcatt gttcagcgga
     4801 tcatcatggt ggattcctat aaaaaacaag gtgtcagtga tctggacgga cgcacagttg
     4861 agagtgatgt tgaagcgttg atgaatgtca accgggacaa tgaagcgctc aacagcgaag
     4921 ccgtcaaaca aggcctcaag caaaaaacac acgctttcta ctcatactac gtcaacctga
     4981 tcagcacagg ccaagtgaaa gcggatattg atctgctgac ttctggcgtt gattttgaca
     5041 tgccggaatg gcttgcatcg tgggaagagg caacaacagg tgcttaccgt gtgaaaagag
     5101 gcttcggaac acacgctgaa atgctgcagg gcgaaacgct agataggaat gccgggattt
     5161 tgctcgaatt tcttaataca caaaccgtaa cggtttctta aatgaagtga tgaaaggagg
     5221 agacggccat gagccaactc ttcaaatcat ttgatgcgtc ggaaaaaacg cagctcatct
     5281 gttttccgtt tgcgggcgga tactcagcgt cgtttcgccc tctccatgct tttttgcagg
     5341 gggagtgtga gatgctcgct gccgagccgc cgggacacgg cacgaatcaa acgtcagcca
     5401 ttgaggatct cgaagagctg accgatttgt acaagcaaga attgaacctt cgtcctgatc
     5461 ggccgtttgt gctgttcgga cacagtatgg gcggaatgat caccttcagg ctggcgcaaa
     5521 agctggagcg tgaaggtatc attccgcagg cagttatcat ttctgcgata cagccgccgc
     5581 atattcagcg gaagaaagtg tctcatctgc ctgatgatca gtttctcgat catattattc
     5641 aattaggcgg aatgcccgcg gagctcgttg aaaataagga agtcatgtcc tttttcctgc
     5701 cttccttcag atcagattac cgggctcttg aacaatttga gcttcacgat gtgaccccga
     5761 tccaatcgcc tgttcatgtc tttaacgggc ttgatgatga aaaatgcatt cgggatgcgg
     5821 aaggctggaa gaagtgggcg aaagacatca cattccatga atttgccggc ggacacatgt
     5881 ttttgctgtc acatacggaa gaagtggcag aacggatttt tgcgatattg aatcagcatc
     5941 cgattattca atcgtgatca aaagcggaca gcgcctgctg ttccgctttt ttgttgagtg
     6001 gcaattttta catgatataa tagtcgcaat actcaaacaa aggcaggtcg aaacatgcgc
     6061 gcgtctccta tgatgaaatg gtttgtattg ctgtatacgt ttgtttttgc catcggattg
     6121 aactcattca gaaattcctt tcaatttttt atgctgccaa tggcggatac cttccatgcc
     6181 gacaggtcgc tgatctcggt ttctgtcagc atttttatga ttacaacggg catcgttcag
     6241 ttttttgtcg gtttttttat cgaccggttc agcgtgagaa agattatggc gcttggcgct
     6301 gtttgtatca gcgcaagctt tttggtgctt ccgtattcac cgaatgttca tgtgttttct
     6361 gccatttacg gcgtgctcgg cggaatcggc tattcctgcg cggtcggcgt gacgacccag
     6421 tatttcatca gccgctggtt tgacacgcac aaaggtctgg cgctcgctat tttgaccaat
     6481 gccaactcag cgggcctgct gctgctctcg cccatttggg ccgcggctcc gtatcatgcc
     6541 ggctggcaaa acacctatac gatattaggc atcgtcatgg cggctgttct gctgccgctt
     6601 ctcgccttcg ggatgaagca ccctccgcat gcacaagcgc agcctgtgaa aaaatcttat
     6661 gactggcggg gattttggaa cgtgatgaag cagtcccgcc ttattcatat cctgtacttc
     6721 ggcgtgttta catgcggatt tacgatgggg atcattgatg ctcatctcgt cccgatgctc
     6781 aaggatgcgc atgtttctca cgtcaacgga atgatggccg cgttcggggc gtttatcatc
     6841 attggcggat tattggcggg ctggctgtca gatttcttcg gcagcagaag cgtgatatta
     6901 tccatcttat ttttcattcg gctgcttagc ctgatttgcc tgctcattcc cattctcgga
     6961 atccatcaca gcgaactttg gtacttcggt tttatcctgt tattcgggct cagttacaca
     7021 ggcgtgatcc cgctgaccgc ggcgtctatt tcagaaagct atcaaacagg attaatcggc
     7081 tcgctgttag gcatcaattt ctttatccat cagatcgccg gtgctctcag tgtgtatgcg
     7141 ggcggtttgt tttatgacat gactcataat tatctgctga ttgttgtggt ttgtgtggtg
     7201 cttgtcggtt tatcagcttt attggagctg gtgccattca aaaacattca ttttcaacac
     7261 aaagagagaa ttgacattta ataaaaaaag aggggacacc cctcttttta agattgcaac
     7321 tttacttttt tggcgggcac atgttctttc acaagctgtc tgaacgagtg ccgctcattt
     7381 tcgaatttga aaaaacgttg aggtatcaaa atcagctggt gactggaggt ttttatggag
     7441 aataagtgtc tatattctgc cgctcccttt atttcattcc attcaaacaa ggtatcatag
     7501 tttttggatt gaatttgaat gccttgccga ttaatggtat atgtcctttt gcgttttaac
     7561 cgctcactgt tcttatagcc ggtacgcagc cgcagatata aaaaggcagt gaagatcagt
     7621 gtgaagatca gagatacaac tgctatgaaa ataccattat aaatcaattc taatgtttcg
     7681 ttgttataaa cggtaaacgg aaatccatat acggaataca ccaatgcaga taggagaaaa
     7741 atataaatat aacaaatcac cgccgctttt ctttgccggt atataaagta atatctcata
     7801 tctcgtaatg aaatggctcc ctctagaaag acgctatgtt gaatcattct tacaatgcac
     7861 ctcaaatata ttttccttca accctaacat aaattctcat ttgttcatag aaatggttcc
     7921 tctttgatgt ttcttctttt tctgcagatt aatattgctc cccagcacgc caataataat
     7981 acaaacggca ccggcaagat gataccaggc caggttttcg tctagaatcg cgactcctgc
     8041 aatcatcgtc acgatggtgg agacatgatt aaaagcgctc attttaaacg cctcaatccg
     8101 agacagcgta tagttggaca gaaacgaagt gacgagtgaa gacagcacgc ccaaatacac
     8161 aatggcgaga acaaagccgg gctcccggaa tggctgaaaa taagtgctgg cagttccagc
     8221 cgtgccgtgg cgcacaaggg cgatggcgtt gaaggcgaca aagccgatcg ctgacatgat
     8281 gtaggtgagc tcggtcagct tgaaccgctg cgtcattttt ctggcagcgg tattgtacat
     8341 cgcggaggac aaggcggaca gcaggatcag caaagatcct tttaagctgg cagattccac
     8401 gtcaacgccc ttcatcacaa aaataaacat gacgccggca acggataaaa ccgtgaaacc
     8461 cttctgcgtc cacgtcgagc gttcctttaa aacataagag gcgaagacca tcgtgaaaat
     8521 cggaatggct gcctgaataa ttcccgcttc agaagaggac gagtatacaa ggccgaatgc
     8581 ctgaaagctg aaaaacaacg ccggatacag cagggcgagc ggcaaaatgg caatgacgtc
     8641 ctttacacgg attgatagct ttacccagcc gaataagacg ggtacagtgg ccgcaacaaa
     8701 cgcaatggtg aaccgatacg ccagaatatc aaacggttcg gctgtttgca gtgcgatttt
     8761 tacgaataga aaggataaac cgataatgaa cgaatataag atagccgcca tataagcgga
     8821 ggcttgctgt ttttttaaca taatgggagg tgctccttta cctgaattgc agcgccggtc
     8881 gctttatttt attgtatggc tgtaaccaga gcggtacaat gagaaaaaca atgaactgta
     8941 ccggtacaaa acagggggag aaagcatgga gaagtatatg agcctattga cgagaataga
     9001 ggagatgatg caaagcagcg cctatcaaga aggtgacagg cttccgtcca tccgtcagct
     9061 gtccgtccgt tatcaagtca gcaaaagcac agtgatccgc gcgctgcagg agctggaaaa
     9121 gcgtcacctt atctactccg ttccgaaaag cggctattat attgtgaaaa agacgggaaa
     9181 agcaaaaagc ggacagcaag gccccattga ctttgccaca tctgcgccgg atcccgatgt
     9241 atttccgtat cttgattttc agcactgcat caacaaagcg attgatacat acaaaaacga
     9301 tttgtttatt tatgggacgc caaaggggct tccatcactc atccgtgtac tccgaaagct
     9361 gctcgccaat cagcaggtat ttgcggatga acggcatatt ttcattacat caggtgtcca
     9421 gcaggcgtta tccctgcttt gtgccatgcc gtttccaaat gggaaagaga agattgcgat
     9481 tgaacagccg ggctatcatt tgatgattga acagcttgaa acgcttggga tacccgccat
     9541 tggagtgaaa agaacggagg aggggcttga tatagccgag attgagcggc tgtttcagac
     9601 agaatcaatc aaattttttt atacgatgcc gcgcttccat aacccgctag gctgttcttt
     9661 atcagagcgt aataaacaag agcttgtgag actggcagaa acgtatgatg tctatctcgt
     9721 tgaggatgat tatctcggtg atctggagga aaataaaaag gcagatccgc tgtacgcata
     9781 tgatctgtcc tcgcatgtca tctatctgaa aagcttctcg aaaatgatgt tccctggcct
     9841 tcgcgtgggg gctgctgttt tgccggatgc gctgattgac acgtttcaca cgtacaaaaa
     9901 gctgaacgac atcgactgct cgatgatttc tcaagcggcg ttggaaattt atctgaaaag
     9961 cggcatgtac ggcaggcata aggagaagat ccgtgcttcc tataaggaac ggtcactaag
    10021 gcttcatcaa gccatccaaa cacataggca gctggggcgc ggccgcttca cgttttccag
    10081 cgggcaggca ccctgcatgc acacccatct ggtgcttcct caggatctgc cggcctcaag
    10141 ggtgattcaa aggctgaaaa aacaaggggt tctccttgag gcgatagacc gtcattattt
    10201 atcagattat ccgaaagaaa atctattaaa aatcaatatt tccaatgtga aaacggaaga
    10261 tattgaacac ggcgtcaagc tgctgctgag ccacttataa aagcgcttcg tacgaaacca
    10321 ttgtgatatc ctcggggaaa tcagggtgcg cggcgcatac ggccattt
//
