LOCUS       NZ_JALAJC010000024      9531 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Bacillus inaquosorum strain S8C1X1 contig_0000024, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAJC010000024
VERSION     NZ_JALAJC010000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 228.72242007437796x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 03:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,337
            Genes (coding)                    :: 4,218
            CDSs (with protein)               :: 4,218
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 82 of 119
            Pseudo Genes (incomplete)         :: 62 of 119
            Pseudo Genes (internal stop)      :: 20 of 119
            Pseudo Genes (multiple problems)  :: 35 of 119
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:37:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9531
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAJC010000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9531
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1290:>9531](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1291..>9531
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            <1..1278
                     /locus_tag="MOB18_RS21770"
                     /old_locus_tag="MOB18_21725"
     CDS             <1..1278
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (0-262). E-value:
                     5.9e-149. Score: 489.1. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21770_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (267-421). E-value: 2.5e-56.
                     Score: 181.8. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21770_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="MOB18_RS21770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21725"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268353837.1"
                     /sec_met_domain="Condensation (E-value: 1.3e-82, bitscore:
                     265.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EKTLQALVEHHDALRMVYREEPKDVVQYNRGLEAASAQLEIIQIE
                     GQAKDYEDRIEREAERLQSSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRIL
                     LEDFAAVYTQLEQGNEPVLPQKTHSFAEYAERVQDFANSKAFLKEKEYWRQLEEQTVAA
                     KLPKDRESGDQRMKHTKTIEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWT
                     GQDQVSVHLEGHGREEIIEDLTISRTVGWFTSMYPMVLDMKHADDLGYQLKQMKEDIRH
                     VPNKGVGYGILRYLTAPEHKEDVAFSIQPDVSFNYLGQFDEMSDAGLFTRSELPSGQSL
                     SPETEKPNALDVVGYIENGKLTMTLAYHSLEFHEKTIKTFSDSFKAHLVRIIEHCLSQD
                     GTELTPSDLGDDDLTLDELDKLMEIF"
     source          1..9531
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="S8C1X1"
                     /submitter_seqid="contig_0000024"
     region          1..9531
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9531
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     aSDomain        4..789
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21770_Epimerization.1"
                     /evalue="5.90E-149"
                     /label="MOB18_RS21770_Epimerization.1"
                     /locus_tag="MOB18_RS21770"
                     /protein_end="262"
                     /protein_start="0"
                     /score="489.1"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EKTLQALVEHHDALRMVYREEPKDVVQYNRGLEAASAQLEIIQIE
                     GQAKDYEDRIEREAERLQSSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRIL
                     LEDFAAVYTQLEQGNEPVLPQKTHSFAEYAERVQDFANSKAFLKEKEYWRQLEEQTVAA
                     KLPKDRESGDQRMKHTKTIEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWT
                     GQDQVSVHLEGHGREEIIEDLTISRTVGWFTSMYPMVLDM"
     aSModule        4..1266
                     /domains="nrpspksdomains_MOB18_RS21770_Epimerization.1"
                     /domains="nrpspksdomains_MOB18_RS21770_TIGR01720.1"
                     /incomplete
                     /locus_tags="MOB18_RS21770"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       262..327
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21770_0001"
                     /evalue="1.30E-12"
                     /label="NRPS-E2"
                     /locus_tag="MOB18_RS21770"
                     /protein_end="108"
                     /protein_start="86"
                     /score="39.7"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       736..783
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21770_0002"
                     /evalue="7.20E-09"
                     /label="NRPS-E5"
                     /locus_tag="MOB18_RS21770"
                     /protein_end="260"
                     /protein_start="244"
                     /score="27.6"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     aSDomain        805..1266
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21770_TIGR01720.1"
                     /evalue="2.50E-56"
                     /label="MOB18_RS21770_TIGR01720.1"
                     /locus_tag="MOB18_RS21770"
                     /protein_end="421"
                     /protein_start="267"
                     /score="181.8"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMTLAYHSLE
                     FHEKTIKTFSDSFKAHLVRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     CDS_motif       829..888
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21770_0003"
                     /evalue="6.90E-10"
                     /label="NRPS-E6"
                     /locus_tag="MOB18_RS21770"
                     /protein_end="295"
                     /protein_start="275"
                     /score="31.1"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        1291..1377
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21775_NRPS-COM_Nterm.1"
                     /evalue="1.40E-14"
                     /label="MOB18_RS21775_NRPS-COM_Nterm.1"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="29"
                     /protein_start="0"
                     /score="45.0"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHS"
     gene            1291..>4383
                     /gene="srfAB"
                     /locus_tag="MOB18_RS21775"
                     /old_locus_tag="MOB18_21730"
                     /pseudo=""
     CDS             1291..>4383
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.4e-14.
                     Score: 45.0. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21775_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-306). E-value:
                     4.6e-116. Score: 379.1. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21775_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     7.4e-113. Score: 369.1. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21775_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1031). E-value: 3.8e-20. Score:
                     63.9. Matches aSDomain: nrpspksdomains_MOB18_RS21775_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="MOB18_RS21775"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21730"
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 2.2e-92, bitscore:
                     297.7, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.1e-117, bitscore:
                     379.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.3e-15, bitscore:
                     43.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFD
                     LEIFEKSVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADRDRQKEHI
                     ERYKQDVQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNY
                     EALRAGRTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPENKDE
                     YVNKEYSFTWDETLVARIQQTANRHQVTGPNLFQAVWGIVLSKYNFTKDVVFGTVVSGR
                     PSEINGIETMAGLFINTIPVRVKVDQDAAFADIFSAVQQHAVEAERYDYVPLYEIQKRS
                     ALDSNLLNHLVAFENYPLDQELENGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFDSAFIERTAEHLTRMMEAAVDQPAAFVREYGLVGDEEQRQIVEVFNSTK
                     AELPEGMGVHQVFEEQAQRTPASTAVVYEGVELTYRELNRAANRLARKLVEQGLQKGET
                     AAIMNDRSAETVVGMLAVLKAGAAYVPLDPALPEDRLRFMAEDSSIRMVLAGKSYTEQA
                     HQLQVPVLTLDSGFEESEAADNLNLPSAPTDLAYIMYTSGSTGKPKGVMIEHKSILRLV
                     KNGGYVPVHEEDCMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLRE
                     QRITAMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYG
                     PTENTTFSTSFLIDREYSGSIPIGKPIGNSTAYIMDEQQRLQPIGAPGELCVGGIGVAR
                     GYVNLPELTEKQFLEDPFRAGERIYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELG
                     EIETKLNMAEHVTEAAVIIRKNKADENEICAYFTADREVSVSGLRKTLSQSLPDYMVPA
                     HLIQMDSLPLTPNGKINKKELPAPQSETAQPEYAAPETESEKKLAEIWEGILGVKAGVT
                     DNFFMIGGHSLKAMMMTAKIQEHFHKEVPIKVLFEKPTIQEL"
     aSDomain        1309..2208
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21775_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="4.60E-116"
                     /label="MOB18_RS21775_Condensation_DCL.1"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="306"
                     /protein_start="6"
                     /score="379.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFDLEIFEK
                     SVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADRDRQKEHIERYKQD
                     VQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAG
                     RTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPENKDEYVNKEY
                     SFTWDETLVARIQQTANRHQVTGPNLFQAVWGIVLSKYNFTKDVVFGTVVSGRPSEING
                     IETMAGLFINTIPVRVKVD"
     aSModule        1309..>4383
                     /complete
                     /domains="nrpspksdomains_MOB18_RS21775_Condensation_DCL.1"
                     /domains="nrpspksdomains_MOB18_RS21775_AMP-binding.1"
                     /domains="nrpspksdomains_MOB18_RS21775_PCP.1"
                     /locus_tags="MOB18_RS21775"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1318..1356
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0001"
                     /evalue="2.40E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="22"
                     /protein_start="9"
                     /score="16.9"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     CDS_motif       1381..1497
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0002"
                     /evalue="2.80E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.3"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     CDS_motif       1714..1779
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0003"
                     /evalue="1.20E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="163"
                     /protein_start="141"
                     /score="37.0"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       1825..1863
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0004"
                     /evalue="1.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="191"
                     /protein_start="178"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       2101..2196
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0005"
                     /evalue="7.50E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       2245..2361
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0006"
                     /evalue="3.70E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="357"
                     /protein_start="318"
                     /score="54.7"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDSNLLNHLVAFENYP"
     aSDomain        2701..3882
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21775_AMP-binding.1"
                     /evalue="7.40E-113"
                     /label="MOB18_RS21775_AMP-binding.1"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="864"
                     /protein_start="470"
                     /score="369.1"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAQRTPASTAVVYEGVELTYRELNRAANRLARKLVEQGLQKG
                     ETAAIMNDRSAETVVGMLAVLKAGAAYVPLDPALPEDRLRFMAEDSSIRMVLAGKSYTE
                     QAHQLQVPVLTLDSGFEESEAADNLNLPSAPTDLAYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNGGYVPVHEEDCMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFL
                     REQRITAMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREYSGSIPIGKPIGNSTAYIMDEQQRLQPIGAPGELCVGGIGV
                     ARGYVNLPELTEKQFLEDPFRAGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       2887..2928
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0007"
                     /evalue="1.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="546"
                     /protein_start="532"
                     /score="21.1"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       3115..3174
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0008"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       3676..3765
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0009"
                     /evalue="2.80E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.4"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGIGVARGYVNLPELTEKQF"
     CDS_motif       3847..3912
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21775_0010"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        4201..>4383
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21775_PCP.1"
                     /evalue="3.80E-20"
                     /label="MOB18_RS21775_PCP.1"
                     /locus_tag="MOB18_RS21775"
                     /protein_end="1031"
                     /protein_start="970"
                     /score="63.9"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVKAGVTDNFFMIGGHSLKAMMMTAKIQEHFHK
                     EVPIKVLFEKPTIQEL"
     gene            4427..4567
                     /gene="comS"
                     /locus_tag="MOB18_RS21780"
                     /old_locus_tag="MOB18_21735"
     CDS             4427..4567
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="MOB18_RS21780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21735"
                     /product="competence protein ComS"
                     /protein_id="WP_225516634.1"
                     /transl_table=11
                     /translation="MNRSSKHLISSIILYPRPSGECISSISLDKQTQATTSPLYFCWRE
                     K"
     gene            <4513..>7503
                     /gene="srfAB"
                     /locus_tag="MOB18_RS21785"
                     /old_locus_tag="MOB18_21740"
                     /pseudo=""
     CDS             4513..>7503
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     3.7e-93. Score: 303.7. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21785_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (427-830). E-value:
                     2.3e-116. Score: 380.7. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21785_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (936-996). E-value: 8.6e-23. Score:
                     72.3. Matches aSDomain: nrpspksdomains_MOB18_RS21785_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="MOB18_RS21785"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="MOB18_21740"
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 5e-86, bitscore:
                     276.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 5.2e-125, bitscore:
                     405.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.8e-15, bitscore:
                     45.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDRDRLENAIQQLINRHEILRTSFDMID
                     GEVVQTVHKNVSFQLEAAKGWEEDAEEMIKAFIQPFELNRAPLVRSKLVQLEEERHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEHADHQKDEAYWLET
                     FKGELPILDLPADFERPAERSFAGERVMFGLDKQSTAQIKSLLAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTMALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFKDETIRRWSSHFVNIIKAAAANPNVRLSDV
                     DLLSPAETAALLEERHMTQITEATFAALFEKQAQQTPDHPAVISGGNLLTYRELDEQAN
                     QLAHHLRAQGAGSEDIVAIVMDRSAEVMVSILGVMKSGAAFLPIDPDTPEERIRYSLED
                     SRARITVVNERNMTAIGQYEGTIVNLDDAAWRNESKERPSPISGSRNLAYVIYTSGTTG
                     KPKGVQIEHRNLTNYVSWFNHEAGLTETDKSVLLSSYAFDLGYTSMFPVLLAGGELHLV
                     PKETYTAPDEIGRYIKEHGITYIKLTPSLFHTMVNTASFTKEPNFESLRLIVLGGEKII
                     PADVLAFRKVYGHTEFINHYGPTETTIGAIAGRVNLSEPGAFAKRPTIGQPIANAGALV
                     LNEALKLVPQGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRL
                     SDGTLEFIGRADDQVKIRGYRIEPKEIETVMLSLSEIHEAVVLAVSEGGLQELCAYYTA
                     DQDIEKATLRNQLSQTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNAAQSGRKASA
                     APETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELEKTVPLKAL
                     FEKPTVRQ"
     aSDomain        4519..5304
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21785_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.70E-93"
                     /label="MOB18_RS21785_Condensation_LCL.1"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="264"
                     /protein_start="2"
                     /score="303.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDRDRLENAIQQLINRHEILRTSFDMIDGE
                     VVQTVHKNVSFQLEAAKGWEEDAEEMIKAFIQPFELNRAPLVRSKLVQLEEERHLLLID
                     MHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEHADHQKDEAYWLETFK
                     GELPILDLPADFERPAERSFAGERVMFGLDKQSTAQIKSLLAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTMALRTA"
     aSModule        4519..7500
                     /complete
                     /domains="nrpspksdomains_MOB18_RS21785_Condensation_LCL.1"
                     /domains="nrpspksdomains_MOB18_RS21785_AMP-binding.1"
                     /domains="nrpspksdomains_MOB18_RS21785_PCP.1"
                     /locus_tags="MOB18_RS21785"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       4522..4635
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0001"
                     /evalue="4.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="41"
                     /protein_start="3"
                     /score="57.7"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDRDRLENAIQQLINRHEILRTSF"
     CDS_motif       4831..4896
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0002"
                     /evalue="8.40E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="128"
                     /protein_start="106"
                     /score="28.0"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       4927..4959
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0003"
                     /evalue="1.30E-03"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="149"
                     /protein_start="138"
                     /score="11.5"
                     /tool="antismash"
                     /translation="HYKDYAVWHKE"
     CDS_motif       5209..5298
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0004"
                     /evalue="2.00E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="262"
                     /protein_start="232"
                     /score="49.0"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTMALR"
     CDS_motif       5353..5466
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0005"
                     /evalue="2.40E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="318"
                     /protein_start="280"
                     /score="52.0"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        5794..7002
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21785_AMP-binding.1"
                     /evalue="2.30E-116"
                     /label="MOB18_RS21785_AMP-binding.1"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="830"
                     /protein_start="427"
                     /score="380.7"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQAQQTPDHPAVISGGNLLTYRELDEQANQLAHHLRAQGAGSE
                     DIVAIVMDRSAEVMVSILGVMKSGAAFLPIDPDTPEERIRYSLEDSRARITVVNERNMT
                     AIGQYEGTIVNLDDAAWRNESKERPSPISGSRNLAYVIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFNHEAGLTETDKSVLLSSYAFDLGYTSMFPVLLAGGELHLVPKETYTAPDEIGRY
                     IKEHGITYIKLTPSLFHTMVNTASFTKEPNFESLRLIVLGGEKIIPADVLAFRKVYGHT
                     EFINHYGPTETTIGAIAGRVNLSEPGAFAKRPTIGQPIANAGALVLNEALKLVPQGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSDGTLEFIGRADDQ
                     VKIR"
     CDS_motif       5980..6021
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0006"
                     /evalue="1.40E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="503"
                     /protein_start="489"
                     /score="15.2"
                     /tool="antismash"
                     /translation="LGVMKSGAAFLPID"
     CDS_motif       6208..6267
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0007"
                     /evalue="9.40E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="585"
                     /protein_start="565"
                     /score="33.5"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       6796..6885
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0008"
                     /evalue="1.10E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="791"
                     /protein_start="761"
                     /score="49.7"
                     /tool="antismash"
                     /translation="PQGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       6967..7032
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21785_0009"
                     /evalue="3.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="840"
                     /protein_start="818"
                     /score="38.2"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        7321..7500
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21785_PCP.1"
                     /evalue="8.60E-23"
                     /label="MOB18_RS21785_PCP.1"
                     /locus_tag="MOB18_RS21785"
                     /protein_end="996"
                     /protein_start="936"
                     /score="72.3"
                     /tool="antismash"
                     /translation="EESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELE
                     KTVPLKALFEKPTVR"
     gene            7576..>9531
                     /locus_tag="MOB18_RS21790"
                     /old_locus_tag="MOB18_21745"
     CDS             7576..>9531
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (1-286). E-value:
                     6.7e-106. Score: 345.6. Matches aSDomain:
                     nrpspksdomains_MOB18_RS21790_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="MOB18_RS21790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOB18_21745"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268353838.1"
                     /sec_met_domain="Condensation (E-value: 6.5e-99, bitscore:
                     319.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1e-49, bitscore:
                     157.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYPLSSAQKRMYVLNQLDRQTISYNMPSVLLMEGELDISRLRGSL
                     NQLVNRHESLRTSFIEANGEPVQRIIEEAVIDLHVFEAEEDEAEQKIKEFIRPFDLNNA
                     PLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFAV
                     WQNEAAQKERMKEHEAYWLSVLSGELPELDLPLDYARPPVQSFKGDTVRFRTGSETAKA
                     VEKLLAETGTTLHMVLHAVFHIFLSKISGQRDIIIGSVTAGRMNADVQDMPGMFVNTLA
                     LRMEAEEQQTFAELLEKAKQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMVTTE
                     NPDKEQLTLQNLSISPYEAHQGTSKFDLTLGGFTDENGIGLQLEYATDLFSRETAEKWS
                     EYVLRLLKAVAENPNQPLASLSIVTETEKQTLLDAWKGKTLPVPTDKTVHQLFEETAQR
                     HKDRPAVTFNGQSWTYGELNAKANRLARILIDCGISADERVGVLTKPSLEMSAAVLGVL
                     KAGAAFVPIDPDYPDQRIDYILQDSGAKLLLKQEGISVPDSFSGDVILLDGDRTILSLP
                     LDENDEGNPEADTNAENLAYMIYTSGTTGQPKGVMVEHHALVNLCFWHHDAFSMTEEDR
                     SAKYAGFGFDASIWEMF"
     aSDomain        7579..8433
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOB18_RS21790_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="6.70E-106"
                     /label="MOB18_RS21790_Condensation_LCL.1"
                     /locus_tag="MOB18_RS21790"
                     /protein_end="286"
                     /protein_start="1"
                     /score="345.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="YPLSSAQKRMYVLNQLDRQTISYNMPSVLLMEGELDISRLRGSLN
                     QLVNRHESLRTSFIEANGEPVQRIIEEAVIDLHVFEAEEDEAEQKIKEFIRPFDLNNAP
                     LIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFAVW
                     QNEAAQKERMKEHEAYWLSVLSGELPELDLPLDYARPPVQSFKGDTVRFRTGSETAKAV
                     EKLLAETGTTLHMVLHAVFHIFLSKISGQRDIIIGSVTAGRMNADVQDMPGMFVNTLAL
                     RMEA"
     CDS_motif       7642..7752
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21790_0001"
                     /evalue="1.10E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOB18_RS21790"
                     /protein_end="59"
                     /protein_start="22"
                     /score="53.2"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISRLRGSLNQLVNRHESLRTSF"
     CDS_motif       7948..8013
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21790_0002"
                     /evalue="1.10E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOB18_RS21790"
                     /protein_end="146"
                     /protein_start="124"
                     /score="30.8"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       8044..8076
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21790_0003"
                     /evalue="8.10E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOB18_RS21790"
                     /protein_end="167"
                     /protein_start="156"
                     /score="15.2"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       8335..8424
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21790_0004"
                     /evalue="1.80E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOB18_RS21790"
                     /protein_end="283"
                     /protein_start="253"
                     /score="42.9"
                     /tool="antismash"
                     /translation="DIIIGSVTAGRMNADVQDMPGMFVNTLALR"
     CDS_motif       8473..8586
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21790_0005"
                     /evalue="3.40E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOB18_RS21790"
                     /protein_end="337"
                     /protein_start="299"
                     /score="58.0"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
     CDS_motif       9115..9156
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21790_0006"
                     /evalue="5.80E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOB18_RS21790"
                     /protein_end="527"
                     /protein_start="513"
                     /score="19.3"
                     /tool="antismash"
                     /translation="LGVLKAGAAFVPID"
     CDS_motif       9358..9417
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOB18_RS21790_0007"
                     /evalue="4.40E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOB18_RS21790"
                     /protein_end="614"
                     /protein_start="594"
                     /score="34.5"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMVEH"
ORIGIN
        1 gagaaaacgt tgcaggcgct ggttgagcac catgatgcac tccgtatggt ctaccgtgag
       61 gagccgaaag acgtcgttca atacaacaga ggacttgaag ctgcttcagc tcaattagag
      121 atcattcaga ttgaaggcca agcgaaagat tatgaagacc gcatagagag agaagcggaa
      181 cggctgcaaa gcagcatcga cttgcaggaa ggcggcttgt taaaagctgg attgttccaa
      241 gcagaagacg gagaccactt gctccttgcc attcaccact tagtggttga cggtgtgtca
      301 tggcggattt tactggagga tttcgccgcg gtttatacac agcttgagca aggcaatgaa
      361 ccggttctcc cgcagaaaac acattcattt gcggagtatg cagagagagt gcaagacttt
      421 gcgaactcca aggcttttct gaaagaaaaa gagtattgga gacagcttga agaacagact
      481 gtcgcggcaa agcttccgaa agatcgtgaa tctggtgatc agcgaatgaa acatacaaag
      541 acaatcgaat tttcgctgac tgctgaagag acagaacagc ttaccacaaa ggtgcatgag
      601 gcatatcaca cagaaatgaa tgatattttg ctgacggcat tcgggttggc gatgaaggag
      661 tggacaggtc aggatcaagt aagtgttcat ttagaggggc atggacgtga agaaatcata
      721 gaagacctga ccatttcccg cacagtcggc tggtttacga gtatgtaccc gatggtgctc
      781 gatatgaagc atgcggatga tctgggctac cagctgaagc aaatgaaaga agatatcaga
      841 catgtgccga ataagggagt cggatacggc attctgcgct atctgacggc gccggaacat
      901 aaagaagatg tggcgttctc gattcagccg gatgtcagct tcaactattt aggacagttc
      961 gatgaaatgt cggatgcagg cttgttcacg agatcagagc tgccgtcagg acagtcgtta
     1021 agcccggaaa cagaaaaacc gaacgcgctc gatgttgtcg gatacatcga aaacggaaaa
     1081 ctgacgatga cactggccta tcattctctt gaatttcatg aaaaaacgat aaagacattc
     1141 agcgacagct ttaaagccca tcttgtcaga atcattgaac attgcttatc tcaagatgga
     1201 acggaactga cgccgagtga ccttggcgat gatgatttga cgctggatga gctggataaa
     1261 ttaatggaaa ttttctaata gaggtggcat atgagcaaaa aatcgattca aaaggtgtac
     1321 gcgctgacac cgatgcagga gggaatgctg tatcatgcga tgcttgatcc gcattcttcc
     1381 tcgtacttca cacaattaga gcttgggatt cacggtgctt ttgatcttga aatctttgag
     1441 aaaagcgtca atgaactgat tcggtcatac gatattctcc gtacggtatt tgtgcaccag
     1501 cagctgcaaa aaccgcgtca ggttgtattg gcggaacgca aaacaaaggt gcactatgaa
     1561 gatatcagtc atgcggaccg agaccgccaa aaagagcaca tcgagcgtta taaacaagac
     1621 gtgcagcgcc aaggttttaa cctggcaaaa gacattttgt ttaaggtggc agttttccgt
     1681 cttgatgcag atcagctgta tcttgtctgg agcaatcatc atattatgat ggacggctgg
     1741 agcatgggcg tactcatgaa aagcctgttc caaaactatg aagcgctcag agcaggccgg
     1801 acaccggcaa acggtcaggg caagccttat tccgactaca tcaagtggct tggaaaacag
     1861 gacaatgaag aagcagagag ctactggagc gaacgcttgg cgggttttga acagccgagc
     1921 gtgctcccgg gccgtctgcc tgagaacaaa gatgaatacg tcaataaaga atattcattt
     1981 acatgggacg aaacactggt tgcccgcatt cagcaaaccg caaaccgcca tcaagtgacg
     2041 gggcctaacc tgtttcaggc tgtttggggc attgttctca gtaaatacaa ctttacgaag
     2101 gacgttgtct tcggcacggt cgtatcggga cggccgtctg aaatcaacgg gattgaaacg
     2161 atggcggggc tgtttatcaa caccattcca gtgcgggtaa aagtagatca agatgctgct
     2221 ttcgctgata ttttctcagc tgttcagcag catgcagtag aggcggagcg ttacgattac
     2281 gtgccgctct atgagattca aaaacgctca gcgctcgaca gcaatctctt gaaccatctg
     2341 gtcgcgtttg aaaattatcc gcttgatcaa gagcttgaaa acggcagcat ggaagatcgc
     2401 ctcgggtttt cgatcaaggt agaaagcgca tttgaacaaa cgagcttcga tttcaacctg
     2461 attgtgtatc cgggcaaaac gtggaccgtc aaaattaaat ataacggagc cgcctttgat
     2521 tccgctttta tcgaacggac agcggagcac cttacccgca tgatggaagc agctgtcgat
     2581 cagccggccg cttttgtgcg tgagtacggg cttgtgggag acgaagagca gcggcaaatt
     2641 gtcgaggtgt ttaacagcac gaaagccgaa ctccctgaag gaatgggggt tcatcaagtg
     2701 tttgaagagc aagcgcaacg gacgccggcg agcactgccg tcgtatatga aggagtcgag
     2761 ctgacgtacc gtgagctgaa tagagcggct aaccgtctgg ctagaaagct tgtcgaacaa
     2821 ggtcttcaaa aaggggaaac agccgcgata atgaacgacc gatcggcaga aaccgttgtc
     2881 ggcatgctgg ctgtgttaaa agcaggcgcc gcctatgtgc cgcttgatcc agcgcttcct
     2941 gaggatcgtc ttcgtttcat ggcggaggac agttccattc ggatggtatt ggccggaaag
     3001 tcttatacag agcaggcaca tcagctgcaa gtgccggtac ttacactgga cagcggtttt
     3061 gaagagagcg aagcggctga caatctcaat ctgccatccg caccgactga tttggcgtac
     3121 attatgtaca catctggttc gacgggcaaa ccgaaaggcg tcatgattga acataaaagc
     3181 attcttcgtc tcgtcaaaaa tggcgggtat gttcctgtcc atgaagagga ctgcatggcg
     3241 caaacgggag ctgtcagctt tgatgccgga acctttgaag tattcggcgc actgctgaat
     3301 ggcgcagcgc tttaccctgt caaaaaagag acgctgcttg atgcgaaaca atttgcagca
     3361 ttcctgcgcg aacagagaat cacggccatg tggctgacat cacctttatt caaccagctt
     3421 gccgcaaagg atgccggcat gttcggcaca ctgcgacatt taattatcgg cggagacgcc
     3481 ctcgtgccgc atattgtcag caaagtgaaa caggcatcgc cgtcgctttc attgtggaac
     3541 ggatacggcc cgactgaaaa cacgacgttt tcaacaagtt ttctgatcga ccgtgagtat
     3601 agcggctcta ttccaatcgg gaaacctatc ggaaattcga ctgcctacat catggatgag
     3661 cagcagcgcc tgcagccaat tggcgcgccc ggcgagctgt gcgtcggcgg aatcggtgta
     3721 gcgcgaggat atgtcaatct ccctgagctg acggagaagc aatttctcga agatccgttc
     3781 agagcgggtg aaaggattta tcgcactggc gacttggcaa gatggctgcc ggacggcaat
     3841 atcgagtttt taggaagaat cgacaatcaa gtgaaggtgc gcggcttccg aattgagctt
     3901 ggcgaaattg aaacaaaact gaacatggca gagcatgtga cagaggctgc cgtgatcatc
     3961 cgcaagaaca aagcagacga aaatgaaatt tgtgcatact ttacggcgga ccgtgaagtg
     4021 tctgtgagcg ggctgagaaa aacactgtct caatctttgc ctgactatat ggttcctgca
     4081 catttgattc agatggacag cctgccgctc acgccaaacg gaaaaatcaa caaaaaagaa
     4141 ctgcctgcgc ctcaatcaga aaccgcgcag ccggagtacg cagcaccaga aacagagagt
     4201 gaaaagaaat tagcggagat ctgggaaggg atactcggcg tcaaagcagg cgttaccgat
     4261 aacttcttta tgatcggcgg tcattctttg aaagcgatga tgatgacggc gaaaatccag
     4321 gagcattttc ataaggaagt accgattaaa gtgctttttg aaaagccgac cattcaagaa
     4381 ctggcgctgt atttggaaga gaacgaaagc aaggaggagc agacgtttga accgatcaag
     4441 caagcacctt atcagcagca ttatcctgta tccccggccc agcggagaat gtatatcctc
     4501 aatcagcttg gacaagcaaa cacaagctac aacgtccccg ctgtactttt gctggaggga
     4561 gaagtagata gagaccggct tgaaaatgcg attcagcaat taatcaaccg gcacgaaatc
     4621 ctccggacat cgtttgacat gatcgatgga gaagttgtac aaaccgttca taaaaacgta
     4681 tcgttccagc tggaggctgc caagggatgg gaagaagacg cggaggaaat gatcaaagcg
     4741 ttcattcagc cgtttgaact aaaccgcgcg ccgctcgtcc gttcgaagct tgtccagctt
     4801 gaggaagaac gccacctgct gctcattgat atgcaccata tcatcactga cggcagttca
     4861 acaggcattc tgatcggtga tctcgccaaa atatatcaag gcgctgatct tgagctgccg
     4921 cagattcact ataaagatta cgctgtttgg cacaaagaac atgctgatca tcaaaaagat
     4981 gaagcatact ggctcgaaac ctttaaaggc gagctgccga ttcttgatct accggcggat
     5041 ttcgaacggc ctgctgaacg gagctttgcg ggagagcgcg taatgttcgg gctcgataag
     5101 caaagcacgg ctcaaatcaa atcactcttg gcagaaacgg atacgacaat gtacatgttt
     5161 ttgctggcag cattcaatgt actcctttct aagtacgcgt cacaggatga tatcattgtc
     5221 ggctcgccga cagcgggaag aacacatcct gatctgcaag gtgtgccggg catgtttgtc
     5281 aacacgatgg cactaagaac ggctcctgcg ggagataaaa cctttgcgca attcctggaa
     5341 gaggtcaaaa cagccagcct tcaagcattc gagcaccaga gctatccgct tgaggagctg
     5401 attgaaaagc ttccgcttac aagggacaca agccgaagtc cgctgttcag cgtgatgttc
     5461 aacatgcaga acatggagat tccttcattg agattaggag atttgaagat ttcttcgtat
     5521 tccatgcttc atcacgtggc gaaattcgat ctctccttgg aagcggtcga gcgtgaagag
     5581 gatatcggcc taagctttga ctatgcgact gctctgttta aggacgaaac catccgccgc
     5641 tggagcagcc actttgtcaa tatcatcaaa gcggccgcgg ctaatccgaa cgttcggctg
     5701 tctgatgtag acttgctttc accagcagaa acggctgctt tgcttgaaga aagacatatg
     5761 actcaaatta ctgaagcaac ctttgcagca cttttcgaaa aacaggccca gcaaacacct
     5821 gatcatcctg cagtgatatc tggcggaaat ctgctgacct atcgcgagct tgatgaacag
     5881 gcgaaccagc tggcgcatca tcttcgtgcg caaggggcag gaagtgaaga catcgtcgcg
     5941 attgtcatgg atcggtcagc tgaggttatg gtatcgattc tcggtgtcat gaagtcgggt
     6001 gcggctttcc ttccgattga tcctgatacg cctgaagaac gaatccgcta ttcattagag
     6061 gacagccgag cgagaattac ggtcgtgaac gaaagaaata tgacggccat tgggcaatat
     6121 gaaggaacaa tcgtcaacct tgatgacgca gcatggagaa atgaaagcaa ggagcgccca
     6181 tcaccgattt ccggatctcg taatcttgca tacgtcattt atacgtcggg tacgaccggg
     6241 aagccgaagg gggtgcagat cgagcatcgc aatctgacaa actatgtttc ttggtttaat
     6301 catgaggcgg gcctgacgga aaccgataag tctgttttgc tttcgtctta cgcattcgac
     6361 ctgggctata cgagcatgtt ccctgttctt ctggcagggg gcgagctcca cctcgtgccg
     6421 aaggaaacct atacggcacc ggatgaaatt ggccgttata tcaaggaaca cgggatcaca
     6481 tatatcaaac tgacaccgtc tctgttccat acgatggtga acacggccag ctttacaaaa
     6541 gagccaaact ttgaatcctt gcgcttgatt gtcttgggag gagaaaaaat catcccggcg
     6601 gatgttctcg ccttccgcaa ggtgtatggg cataccgaat ttatcaatca ctacggcccg
     6661 actgaaacaa cgatcggcgc catcgccgga cgggttaatc tgtctgaacc gggcgcattc
     6721 gcgaaacgcc cgacaatcgg acagccgatt gcgaatgccg gcgcgcttgt cttaaatgag
     6781 gcgttaaagc ttgtgcctca aggagcgagc ggccagctct atatcacggg gcaggggctt
     6841 gcgagaggat atctcaacag gcctcagctg acagccgaga gatttgtaga aaatccatat
     6901 tcaccgggca gcctcatgta caaaaccgga gatgtcgtac gaagactttc tgacggtacg
     6961 cttgaattta tcggccgggc tgatgatcag gtgaaaatcc gcggctaccg cattgagccg
     7021 aaagaaattg aaacggttat gctcagcctc agcgaaattc atgaagcggt tgtgctagcg
     7081 gtttccgagg gcggacttca agagctttgc gcgtattata cggcggatca agacattgaa
     7141 aaagcaacgc tccggaacca gctttcacaa acactgccgt ctcatatgat tcctgctttc
     7201 tttgtgcagg tggacgcgat tccgctgacg gcaaacggga aaaccgacag aaacgcattg
     7261 ccgaagccga acgcggcaca atccggacgc aaggcctcag ccgcaccgga aacagcgctt
     7321 gaagagagcc tatgccgcat ttggcagaaa acgcttggca tagaagcgat cggcatcgac
     7381 gacaatttct tcgatttagg cggccattca ttaaaaggaa tgatgctcat cgccaacatt
     7441 caggccgaat tggagaaaac cgtaccgctt aaagcactgt tcgagaagcc gactgttcgc
     7501 cagcttgcgg cttatatgga ggcatcggca gtttcaggcg gccatcatgt gctcaaaccg
     7561 gctgacaaac aggatatgta cccattgtca tccgcgcaga aacggatgta tgtactgaat
     7621 cagcttgacc ggcagacgat cagctacaac atgccatctg tccttctgat ggaaggcgag
     7681 cttgatattt cacgtctgcg cggctcactg aatcagcttg tgaaccggca cgagtcattg
     7741 cgcacatcat ttatagaagc caatggtgag cctgttcagc gcattattga ggaggcggtg
     7801 attgatcttc atgtgtttga agcggaagaa gacgaagcgg aacaaaagat caaggaattt
     7861 atccggccat tcgacctaaa caatgcgccg ctcattcgtg cggctttgct tcgaatagaa
     7921 gcgaagaaac acttgctgct tttggatatg catcacatca tcgctgacgg cgtatcaaga
     7981 ggcatttttg taaaagaatt ggcgctgctt tacaaaggag aacagcttcc ggagccggcg
     8041 cttcattata aggatttcgc cgtttggcaa aatgaagctg cgcaaaagga acggatgaag
     8101 gagcatgagg cgtactggct ctcggttctt tcaggcgagc tgccggagct tgatcttccg
     8161 ctcgattacg cccgtccgcc tgtgcaaagc tttaaaggag acacggtccg tttccgtacg
     8221 ggaagcgaga cggcaaaggc ggtagagaaa ctgcttgcag aaaccggaac gaccttgcac
     8281 atggtgcttc acgctgtttt ccacatcttt ttaagcaaaa tttccggaca gcgggatatc
     8341 atcatcggct ccgtcactgc cggccggatg aatgccgatg tccaagacat gccggggatg
     8401 tttgtcaata cgcttgcctt aagaatggaa gcggaagaac agcaaacctt tgcagagctt
     8461 ctggagaaag cgaagcagac gaacctgtca gcgcttgaac atcaggaata tccgtttgag
     8521 gacttggtca atcagcttga tcttcctcgg gatatgagcc gaaatccatt gtttaacgta
     8581 atggtgacga cagaaaatcc tgataaagaa cagcttacat tgcagaacct aagcatttca
     8641 ccttatgagg cccatcaggg aacgtctaag tttgatctga cattaggcgg attcactgat
     8701 gaaaacggca tcggcctgca gcttgaatat gctactgatc tgttctcaag agagacggct
     8761 gagaaatgga gcgaatacgt tctccgttta ctaaaggctg ttgcggaaaa tccgaaccag
     8821 ccgcttgcca gcctgtcaat cgtcacggaa acagaaaaac aaacgcttct cgatgcatgg
     8881 aagggcaaaa cgctccctgt gccgacagac aaaaccgttc atcagctatt cgaagagacc
     8941 gctcagcgcc acaaagaccg cccggctgtc acattcaacg gacaatcatg gacgtacggg
     9001 gagctgaatg cgaaggcaaa ccgtcttgcg cggattctga tcgactgcgg catcagcgcg
     9061 gatgagcgcg tcggcgtcct cacgaagccg tcgcttgaaa tgtccgctgc ggtgctcggc
     9121 gtcttaaagg cgggggcggc gtttgtgccg attgatcctg actatccgga tcagaggatt
     9181 gactatattt tacaagacag cggcgcgaag cttctcttga aacaggaagg tatttcagtt
     9241 ccggatagct tctcaggaga cgtcattctt ctcgacgggg atcgcacgat tctaagcctg
     9301 ccgcttgatg aaaatgatga gggaaatcca gaggcggata caaacgcgga gaacctcgcg
     9361 tacatgattt acacgtctgg aacgaccgga cagccgaagg gtgtcatggt tgagcaccat
     9421 gcgcttgtga acctgtgctt ctggcaccac gacgcgttca gcatgacaga ggaggaccgc
     9481 agtgcgaaat acgcgggctt tggattcgat gcctccattt gggagatgtt c
//
