LOCUS       NZ_JAPDMW010000014     12175 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Paenibacillus alvei strain LF-11/2021
            NODE_14_length_154760_cov_43.997439, whole genome shotgun sequence.
ACCESSION   NZ_JAPDMW010000014
VERSION     NZ_JAPDMW010000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus alvei
  ORGANISM  Paenibacillus alvei
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 154760)
  AUTHORS   Fallico,L.
  TITLE     Analysis of the Paenibacillus alvei's inhibition ability against
            multidrug resistant bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 154760)
  AUTHORS   Fallico,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) U.O.C. Microbiologia, Ospedale San Bortolo
            AULSS8 BERICA, Viale F. Rodolfi, 37, Vicenza, Vicenza 36100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 71.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 02:12:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,998
            CDSs (total)                      :: 5,902
            Genes (coding)                    :: 5,824
            CDSs (with protein)               :: 5,824
            Genes (RNA)                       :: 96
            rRNAs                             :: 9, 1, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 1, 8 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 65 of 78
            Pseudo Genes (internal stop)      :: 14 of 78
            Pseudo Genes (multiple problems)  :: 27 of 78
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:37:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 140051
            Orig. end                         :: 152226
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPDMW010000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12175
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12175
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12175
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:7175](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7175
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(251..267)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.25"
                     /tool="antismash"
     misc_feature    375..386
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.77"
                     /tool="antismash"
     gene            433..1290
                     /locus_tag="OF778_RS23085"
     CDS             433..1290
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543236.1"
                     /locus_tag="OF778_RS23085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PRD domain-containing protein"
                     /protein_id="WP_268626416.1"
                     /transl_table=11
                     /translation="MSEQRRFEIVRSLSNNVVLANDVLANKETILMGKGLGFGAKPGGS
                     LETSDTRIEKTFVIEDKQHLSQYQMLLGQIDPEVIVISEKIISIVSEQLTPDLNEHIHL
                     ALPSHIEYALYRLRNNIAIENPFLWEIRTLNPKEFELASQAAELINKTFDVEVPEDEIG
                     FLTIHIQSAVAHVPVGNFVQYNHLLKDLVALIERERGRVIPKDSIDYLRLVTHLRFAME
                     RIRQGDATKNPFRDNLKEMAPYEFSVAQKCADVMSKKLEIEIPEDETAYIAMHLYRLFH
                     KDSE"
     gene            1499..2941
                     /locus_tag="OF778_RS23090"
     CDS             1499..2941
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1145: Glucose-specific phosphotransferase enzyme IIA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683819.1"
                     /locus_tag="OF778_RS23090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PTS transporter subunit EIIC"
                     /protein_id="WP_268626417.1"
                     /transl_table=11
                     /translation="MLAFLQKIGRALMLPVATLPAAAILLRFGAIDYVKDFHMGAAGEF
                     LNHYVAPFLHAGGDTIFKQLPLIFAVAIAIGLAGDAVAALAAVLAHIILMKILEITPGI
                     FSFIPDDLKLDMGVLGGILAGCLAAFMYNRYHNIKLPDWLGFFGGKRFVPMVTSISIII
                     IGLAFAIVWGPVQQAIDSFGNWVVGLGAIGSGGFMLANRLLIPTGLHHILNNIAWFQLG
                     TFTDAAGEVVKGDISRFFAGDKSAGMFMTGFFPIMMFALPGAALAIIHTARPEKRKAVA
                     SIFMGVAFASFLTGVTEPIEFAFMFAAPVLFVVHAVLSGISGVITALWGIKHGFGFSAG
                     FIDYALNFPLATKPLLLIPIGIAYFVVYYLLFRFLIVKLNLKTPGREDDGEGAKAEAGG
                     GNSSLQEKAVKVLGMIGGKDNIIVNEACITRLRLTLKDDTVVDEAGLKSLGAAGIMKLG
                     KGNVQVVFGTQSELLKEEINKL"
     misc_feature    complement(1508..1521)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.25"
                     /tool="antismash"
     misc_feature    3207..3223
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.95"
                     /tool="antismash"
     gene            3242..3430
                     /locus_tag="OF778_RS23095"
     CDS             3242..3430
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF778_RS23095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_268626418.1"
                     /transl_table=11
                     /translation="MKSLKNNNTLFMELDEEQLLLINGGGNYPKEITGGSAGIAWGIAN
                     AGEPTTGSYQTTWSYRK"
     gene            3506..4906
                     /locus_tag="OF778_RS23100"
     CDS             3506..4906
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /locus_tag="OF778_RS23100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_268626419.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.1e-21,
                     bitscore: 69.6, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKINIDGYKFNRGYLMKLYEYLIANDTKHNTRLLASIKERIRLLG
                     YIYKCSDEISEFLTGDDSSIDLCQENSSCMLDAIENNLEIKPLKISVNIITYNEERCIK
                     RCIDSIKCLADEILVLDTGSNDNTVSIIEGEYPQVKLFQCKWGDDFSAARNKLINMSTG
                     DWIFQIDADEYLVSSIADVRNFLGMFNDFPELMTVISPKVINHDNSELLLSKRMFKKES
                     GLKYYGIIHEELRSSDIHKRGEDISHIGSEFVLKHDGYRKEIVSKKKKLERNKNLLEKM
                     IEIEPDNIRWHYFLAREKRGLKESNEQILQILSNGIKFKGYNKDLDHFYLAALVLMAEI
                     FHEDGYEDKLKDTIHTINCNYPYCIDGIYFALLHEYQKSSINLYKSAQESIARMKKMDE
                     QLSFIHSKGYHIINLVALLYFHKQDYAAAFRIFSTIEDETYLKDMKIKMIDLKNQIETF
                     LENLKHIT"
     gene            5001..7175
                     /locus_tag="OF778_RS23105"
     CDS             5001..7175
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009454.1"
                     /locus_tag="OF778_RS23105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_268626420.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.3e-43, bitscore:
                     139.8, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 1.2e-203, bitscore:
                     671.4, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFRKYKVVLQHDEKDCGPACLATICKQYGLNYPISKLREMSGTDK
                     NGTSAYGLIQAAQQLGFTARGVKGNIDFLKSGMEFPIIAHVVTKDLFPHYIVIHSVEKN
                     KLIVADPAKGMTKYDINDFLETWTGVLILLHPNPSFQQKNCKKSNLGLFKLLQNQRKRI
                     LLVFIISILYTAFGILSAFYFKVLLDDILPVHEVKYLHVLSISIILLIVVRSFFHFWRR
                     KQIIKMGTEIDYKLVVDYYKHLFKLPLQFFGSRKHGEIVSRLTDIATIRSAVSSTAFTI
                     LIDTVMLFAGGIVLYKQNKLLFSITLLLVPIYVVILLMYNTRFESLNREQMKKNATLMS
                     YAIETIKGIETIKANGAEKKVEQNMKLKLNDFLLEVVSLSQAENKQNTLKFLIQGISGI
                     LILWIGSYQVINGEISIGELISYNALLIYFLEPLQNLINLQPTLKSADVALNRLTEILD
                     IEQEDAVDTDNRDDEIISLKQSIYFDGVYFRYGTRNMNLFDISLKINHGEKVAIVGESG
                     SGKSTLVKLLLRFYNPESGRIIFGDKDITTIDRALLRNKISYIPQENFFFTGTIKDNLS
                     LGLQNNSVSFEKMTVAAKKAGIHDFITELPLQYNTILEEGASNLSGGQKQRLAIARALL
                     REPDILIMDEATSNLDSITEQHISKMINSCSGITTIIVAHRLNTVIDCDNIFVLDKGKL
                     LENGTHGQLVANRGKYFELWSSQNHDLMYK"
     gene            complement(7587..8903)
                     /locus_tag="OF778_RS23110"
     CDS             complement(7587..8903)
                     /EC_number="5.4.3.8"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA]"
                     /GO_process="GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (40-359). E-value: 1e-66.
                     Score: 217.2. Matches aSDomain:
                     nrpspksdomains_OF778_RS23110_Aminotran_3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543232.1"
                     /locus_tag="OF778_RS23110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="WP_268626421.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.7e-72, bitscore:
                     235.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTNRNRSAQLYEEALQHIVGGVNSPSRSFKAVGGGAPVFMERAQ
                     GAYFWDVDGNRYVDYLAAYGPIITGHAHPHVTKAIVRAAQNGTLYGTPTELEIKLARML
                     KEAVPSMDKVRFVNSGTEAVMTTIRVARAYTKRTKVIKFAGCYHGHSDLVLVAAGSGPS
                     TLGIPDSAGIPSSIADEVITVPFNDLDSLKEALEHWGDDVAAVMVEPIVGNFGMVMPAE
                     GFLEGLCKLAREYGALVIYDEVISAFRFHYGSAQTYRAFPVFAAIEPDLTAMGKIIGGG
                     LPIGAYGGRKHIMEQVAPLGPAYQAGTMAGNPASISAGIACLEVLQAEGVYEQMEELAI
                     RLTNGIQEGADRYGVPLTINRIRGSFSTHFCDHPVTNYAEAQETDTEAFGAFFRHMLDQ
                     GICLAPSKYEAWFLTTEHTAEDIERTIEAAHEAFRRMRG"
     aSDomain        complement(7827..8783)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_OF778_RS23110_Aminotran_3.1"
                     /evalue="1.00E-66"
                     /label="OF778_RS23110_Aminotran_3.1"
                     /locus_tag="OF778_RS23110"
                     /protein_end="359"
                     /protein_start="40"
                     /score="217.2"
                     /tool="antismash"
                     /translation="MERAQGAYFWDVDGNRYVDYLAAYGPIITGHAHPHVTKAIVRAAQ
                     NGTLYGTPTELEIKLARMLKEAVPSMDKVRFVNSGTEAVMTTIRVARAYTKRTKVIKFA
                     GCYHGHSDLVLVAAGSGPSTLGIPDSAGIPSSIADEVITVPFNDLDSLKEALEHWGDDV
                     AAVMVEPIVGNFGMVMPAEGFLEGLCKLAREYGALVIYDEVISAFRFHYGSAQTYRAFP
                     VFAAIEPDLTAMGKIIGGGLPIGAYGGRKHIMEQVAPLGPAYQAGTMAGNPASISAGIA
                     CLEVLQAEGVYEQMEELAIRLTNGIQEGADRYGVPLTI"
     gene            complement(8919..9953)
                     /locus_tag="OF778_RS23115"
     CDS             complement(8919..9953)
                     /GO_process="GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543231.1"
                     /locus_tag="OF778_RS23115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LCP family protein"
                     /protein_id="WP_268626422.1"
                     /transl_table=11
                     /translation="MTLAALPPDAERTIHMLKKYWKHALISVCVFLLASAGYYMWSMYS
                     SLDSLQKPPETSRFTPVVHRVPEKVDIEPPKWQGEERVNILLLGGDSRGLEANEVPRSD
                     SILLASFDPKTKRANLFSLLRDTYVDIPGFGKDRLNAALAYGGPELAMKTASDLTGLDI
                     QYYVYTDFVGFIKLIDALGGVEFEVEKDMKYTSKADKHQYDIDLKKGKQLLNGETALMY
                     ARFRYDAMSDYARTQRQRDLLGALANKLKSTWNLLQLPSLIREVSPYVETNLSADDIVK
                     LAALGYDSSIGQSHQLPPMDNVTDARVNNAAVLRVIDDDKLKQYIQKALEVNVTQSVSE
                     TNSQ"
     misc_feature    complement(10094..10109)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     misc_feature    10131..10142
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            10135..11286
                     /locus_tag="OF778_RS23120"
     CDS             10135..11286
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421330.1"
                     /locus_tag="OF778_RS23120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_268626423.1"
                     /transl_table=11
                     /translation="MNAIDVRDLRKTFQVQKNRDGLKGALLDLFKREYNEVMAVKDISF
                     TIPQGEICGYIGENGAGKSTTIKMLTGILVPTSGDIRVNGFIPHKEREKFVQGIGVVFG
                     QRSQLWWDIGVIESFRLLRKVYRVSEHDFKQRLDELVERLALQELLNRPVRKLSLGQRM
                     RCELVASLLHRPSVLFLDEPTIGLDIVVKTEIREFLKQLNAEHGTTILLTTHDLQDIEA
                     LCTRVIMLDDGRIIYDGGLDTLKSTWGKGREVRFQFAKPMRAGDVEELTSGLDVEWTVE
                     SPYEAGVLVPFATNVSDVIGRVVGGASISDLSIIETNTDDIVREIYKAGTAEKPEGLSP
                     LLAVQSKMEVESSSSDTDVNTGDSNRKEENDKEKEEKEAVTHG"
     gene            11279..12073
                     /locus_tag="OF778_RS23125"
     CDS             11279..12073
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007428811.1"
                     /locus_tag="OF778_RS23125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC-2 family transporter protein"
                     /protein_id="WP_268626424.1"
                     /transl_table=11
                     /translation="MASAYLEFIRIRFLLMLAYRVNYYSGILIYTLNIGVYYFTWQAIY
                     GGQGTLAGFTADQMTTYIAVSWMARAFYFNNMDRDIAMEIRDGSVAIQFIRPYNYLFVK
                     LMQAFGEGLFRLLLFMGPGMVIACILFPVKLPQDPKQWAVFGVMLFMSFLINSQLNMLT
                     GLTAFFIENNEGMIRMKRVVVDLFSGVIVPISFFPGWLLVLNEWLPFQAITYLPSSVFT
                     GRVVGDQVWAALGIQLLWAVVLVIPIVLLWRSARRRLFVQGG"
ORIGIN
        1 cttggtcttg atgaattcag catgagtgca agctctgtac ttccagtacg tgtactgctg
       61 agccgcttga accgtgaaga gatgcgcaag ctagcggaag aagcgttgat gctggagacg
      121 gctgaagaga ttcaagttat ggtcgttgac cgtgtatcag cagttcaaga aatgtctatc
      181 taaagtaacg aatgtattgc agtcgggtgg ggataagctc ctttcccggc tgcctcatcg
      241 tttacatatg tgtcaataaa attacaaata aataatattc tgcaataatt ctacatattc
      301 gacaaaatta gatttattcg ttgacattga ttgtagattc acctagaatg aatgatgtag
      361 ttgacgcacg aatcgaaaac gtttccaaaa cgtaatcttc catgtgtacg ctcaggggaa
      421 aggagccttg ctttgtcaga acaacgtcgc tttgaaatcg tacggtcatt aagcaacaat
      481 gtcgtattgg caaacgatgt gttggctaac aaagaaacca tccttatggg gaaagggctc
      541 ggctttgggg caaagcctgg aggttctttg gagacgagtg atacacgaat cgagaagact
      601 tttgtgatcg aggacaaaca gcatctatcg cagtatcaga tgctcctcgg gcaaattgat
      661 cccgaagtca ttgtcatttc ggagaagatt atttcgatcg tttcggagca gttgacgccg
      721 gatttgaacg agcatattca tctcgctttg ccgagccata tcgaatatgc gctttatcgt
      781 ctccgcaaca atatagcgat tgagaatccg tttctatggg agattcgcac attgaacccg
      841 aaggaattcg aactcgcgtc tcaagcagct gagctgatta ataagacgtt tgatgtagag
      901 gtaccggagg atgagattgg cttcttgacg atccatattc agtctgcggt tgcccatgtc
      961 cctgtcggga actttgttca atataatcat cttctgaagg atttggtcgc tctcatcgaa
     1021 cgcgagcgag gcagggtcat tccgaaggat agcattgatt atttacgatt agttacgcat
     1081 ttgcgctttg cgatggaacg aattcgccag ggtgatgcca cgaagaatcc attccgagac
     1141 aacttgaagg aaatggcgcc ttacgaattt agtgtcgcac agaaatgtgc ggacgtgatg
     1201 tcgaagaagc tggagatcga aatacccgaa gatgaaacgg cgtacatcgc aatgcattta
     1261 tatcgtttat ttcataaaga ctcagaataa cgctggacaa gcgtgttact agttcgacta
     1321 ggcatgagct tgctggggga taacatctaa tttttttgga tgtcgtcccg tcgcaagctc
     1381 atgccttttt tattttctct aatgcgctta cattttggcg gaggagatga caggtcagag
     1441 cataaatttt tgacctttta tccatacttt acttattcac cacaaggagg aacgacatat
     1501 gctagccttt ttgcaaaaaa ttggtcgtgc attgatgctt ccggttgcaa ctctgccggc
     1561 agcggcaatt ttacttcgat tcggcgctat tgactacgtg aaagattttc acatgggcgc
     1621 tgcaggcgag tttttgaacc attatgtagc tccattttta catgcaggtg gggataccat
     1681 ctttaaacaa ttaccgctaa tatttgcagt agccattgca ataggattag cgggagatgc
     1741 agttgctgca ttagcggctg tgcttgctca tatcattctt atgaaaatat tggagatcac
     1801 tcctggtatc ttcagtttca tcccggatga cttgaaattg gatatgggtg tattaggcgg
     1861 tatcttagca ggatgtttag ctgcctttat gtacaacagg tatcataata tcaagctgcc
     1921 tgattggcta ggattctttg gcggaaaacg atttgttccg atggtaacat ccatttcgat
     1981 tattattatc ggtcttgctt ttgcgattgt ttggggccca gtacaacaag ctatcgattc
     2041 atttggtaac tgggttgttg gtttaggtgc aatcggttct ggcggcttta tgttggctaa
     2101 tcgcttgctt attccaacag gcttgcatca tatccttaac aacattgctt ggttccaact
     2161 cggtacattt acagatgccg ctggcgaggt cgtgaaaggt gatatttctc gtttcttcgc
     2221 aggggacaag tcagctggta tgtttatgac aggtttcttc ccgattatga tgtttgcttt
     2281 gcctggtgca gcacttgcca ttattcacac tgcacgtcct gaaaaacgca aagctgtagc
     2341 atcgatcttt atgggagtgg catttgcttc tttcttaaca ggtgttacag aaccgattga
     2401 gtttgcgttc atgtttgcag ctccagtatt gttcgtagtg catgctgtgt tgtccggtat
     2461 ctccggcgta attacggcat tgtggggaat caagcacggc tttggtttct cggcagggtt
     2521 tatcgactat gcgctgaact tcccgcttgc gactaagcca ctgttactca ttccaatcgg
     2581 tattgcatac ttcgttgttt attacctctt attccgcttc cttatcgtga agctgaacct
     2641 gaagacgcct ggacgcgaag atgacggtga aggcgccaag gctgaagctg gcggcggcaa
     2701 ttcatctctg caagagaagg ctgtgaaagt gctggggatg attggcggca aagacaacat
     2761 cattgtcaac gaagcctgta tcacgcgcct tcgcttgacg ctgaaggatg acacggtagt
     2821 ggatgaagct ggcctaaaat cccttggtgc ggccggtatc atgaaactag gcaagggtaa
     2881 cgtgcaagtt gtattcggca cacaatccga gcttttgaaa gaggaaataa acaagttata
     2941 attttagcaa tttatgcagg cgattgcctt cgcaaatatt gagattttaa gcgatcaagc
     3001 aaagaaaatt gtagttactt aataacctgg catcggcata taaaccgatg ctaggttatt
     3061 ttgagaaaaa ataaaagtaa ttaataatat ccatatttaa tgatgccagg aaaatggcaa
     3121 tcacgataat aagtaatata ttaccaatga atatatgatc atataataaa aattgcgttc
     3181 gttttgtgtt ataatgtaat tgtaactagt tacaaaataa caaaaaaggg gaaatatgaa
     3241 tatgaaatcc ttgaagaata acaacacgtt gtttatggaa cttgatgaag aacaattgct
     3301 tcttatcaat ggtggaggaa actaccctaa agaaataacg ggtggatccg cagggatcgc
     3361 atggggaatt gcaaatgcag gggaacctac aacaggaagt tatcaaacaa cttggtctta
     3421 tcgcaaataa taaacgaata atttaaataa aaggctgagt ctatcagcct tttatttatc
     3481 ataaatatat agttggtgaa caattatgaa gataaatatt gatgggtata aattcaaccg
     3541 tggttatcta atgaagttgt atgagtattt gattgcgaat gatacgaaac ataatactag
     3601 attactcgct agtataaagg agagaattcg tctcttaggt tatatatata agtgttcaga
     3661 tgaaatatcc gaatttttaa cgggcgatga ttcgagtatt gatttatgtc aagaaaattc
     3721 gagctgtatg ctagatgcaa ttgaaaataa tctagaaata aaaccgctta aaatttctgt
     3781 gaatattatt acatataatg aggagagatg catcaagaga tgtattgaca gcattaaatg
     3841 tcttgcggac gaaatattag ttcttgatac gggatcgaat gataatacag taagcattat
     3901 tgaaggagag tatccacaag taaagttgtt tcaatgcaaa tggggtgatg atttttcagc
     3961 ggctagaaat aaattaatta acatgtcgac tggcgactgg atatttcaga ttgatgctga
     4021 cgagtattta gttagcagta tagctgatgt aagaaatttt ttaggaatgt tcaatgattt
     4081 tcctgaatta atgacggtaa ttagtcctaa agttattaat catgataaca gtgaattatt
     4141 gctatctaag aggatgttca aaaaagagag tggattaaag tattacggaa ttatccatga
     4201 ggaacttagg agtagcgata tacataaaag aggagaggac atatcccaca ttggttccga
     4261 atttgttctc aagcatgacg ggtacagaaa agagattgtt tcaaaaaaga aaaaattaga
     4321 aagaaataag aatctattag aaaaaatgat tgagattgag cctgataata ttagatggca
     4381 ttatttcctg gcaagagaaa aaagagggct gaaagaatct aacgaacaaa ttctccaaat
     4441 attatcaaat gggataaaat tcaaaggtta caataaagat ttggatcatt tttacctagc
     4501 agcattagta ttgatggcag aaatatttca tgaagatggt tatgaagata aattgaagga
     4561 tacaatccac acaattaatt gcaattatcc ttattgcatt gatggaatat attttgcttt
     4621 attacatgaa tatcagaaat catctataaa tttatataaa tcggctcaag aatctattgc
     4681 gagaatgaaa aagatggatg aacagttatc ttttattcat tctaaaggtt accatatcat
     4741 taatctcgtg gcacttttat atttccataa gcaagattac gctgctgctt ttcgtatatt
     4801 tagtacaata gaggatgaga cgtacttgaa agatatgaaa attaaaatga ttgatctgaa
     4861 gaaccaaatt gagacatttt tagagaacct gaagcacata acataaaaaa taactcatct
     4921 ggagcctttc gaaatggaga gattgagaag caatcattct attttccata tcgaaaggat
     4981 ttttatttta ggagatatat atgtttcgta aatataaggt agttttacag catgacgaaa
     5041 aggattgtgg tcctgcgtgc cttgcaacaa tttgcaagca atacggatta aattacccga
     5101 tttctaaatt acgcgaaatg tcaggtacag acaagaatgg aaccagtgct tatggtttga
     5161 tacaagcagc ccaacagtta ggatttacag cacgtggggt aaagggaaat atagattttc
     5221 ttaaaagcgg aatggaattc cccattattg cccatgtagt caccaaagat ttatttcccc
     5281 actatatagt catacatagt gtagagaaaa ataagcttat tgttgctgat ccggcaaaag
     5341 gaatgacaaa gtatgatatt aatgattttt tggagacttg gacaggggtg ttgattttat
     5401 tgcaccccaa tccttcattc cagcaaaaaa actgtaagaa aagcaacttg ggtcttttta
     5461 aattgttaca gaatcaaagg aagagaattc tgctggtttt tataatatct atattgtata
     5521 cagcttttgg aatactctcc gcgttctatt ttaaagttct gcttgatgat atattgccag
     5581 ttcatgaagt aaaatacctt catgtccttt caatttctat aatccttcta atagtagtca
     5641 gatcattttt ccatttttgg cgcagaaaac aaatcatcaa aatgggtaca gaaatagact
     5701 ataaattagt tgttgattat tataaacatc tttttaagtt accgctgcaa ttcttcggtt
     5761 caagaaagca tggcgagata gtctcacgtc taactgatat agccacgatt agatccgccg
     5821 tatcaagtac ggcgtttaca attctaattg atactgtaat gttgtttgca gggggaatcg
     5881 ttctatacaa gcaaaataaa cttctattta gcattacact tctattagtc cctatatatg
     5941 tagttatact gctaatgtac aatactcgat ttgagtcttt gaacagggaa caaatgaaga
     6001 aaaatgcaac tttaatgtct tatgcaattg agactattaa agggattgaa accatcaagg
     6061 caaatggcgc tgaaaaaaaa gttgagcaga atatgaagct taaactaaat gattttttgc
     6121 ttgaggtagt aagtcttagt caggcagaga ataaacaaaa cactttgaaa tttttgatcc
     6181 aaggtataag cggaattttg atattatgga ttgggtctta tcaggtaatt aatggggaaa
     6241 tttcaatagg ggaactgatt tcatacaatg cgctattgat ctatttctta gaacctctcc
     6301 aaaatttgat aaacttgcag cctacattaa aaagtgccga tgtggctttg aataggctga
     6361 cagaaattct tgatatagag caggaagatg cagtagacac ggacaataga gacgacgaga
     6421 ttatttcgtt gaaacaatca atatattttg atggcgtata tttccgctac ggtacaagaa
     6481 atatgaatct atttgatata tcactaaaga taaatcatgg tgaaaaagta gcaatagtcg
     6541 gggagagtgg atcaggtaag tcgaccctcg ttaaactgtt acttcgattc tacaatccgg
     6601 aaagcggaag gattattttt ggcgataaag atataactac tattgatcga gcattattac
     6661 gaaacaagat ttcatatata ccacaagaaa actttttttt tacgggaacc ataaaggata
     6721 acttaagttt gggattgcaa aataatagcg tatcatttga aaaaatgacg gttgctgcga
     6781 aaaaagccgg cattcatgat ttcataaccg aattgccctt gcaatacaat acaatattag
     6841 aagaaggcgc ttcaaattta tctggcggtc agaagcagcg cttagcgatc gccagggcct
     6901 tgctccggga accggatata ttgataatgg atgaggctac aagcaattta gattctataa
     6961 cggaacaaca tatctcaaaa atgattaatt cttgctctgg aataacaacg attatagttg
     7021 ctcatcgatt aaatacagtg attgattgcg ataacatctt tgtattggat aaaggaaaat
     7081 tactcgaaaa tggtacacat gggcaactcg ttgcaaatag aggtaagtat tttgaactat
     7141 ggagtagcca aaatcatgat ttgatgtata aataagttac atcgaatttg tgaattaaca
     7201 tctaacggtt cgctgcttcc atatgaatgc gaacaaatta tttctttgag aatgtctcct
     7261 ttaaatgctt ggcaggataa agaatgtctc tttttcgatt atgatcggtg tatctgaaac
     7321 tgctgatgct aatcaggtaa gaaagcaggc caggaagctg ttacgtatgc tgcatccaga
     7381 tcatggtgga agcacatgtt tgttcgcgtg gttgaaggaa gactatgatg cctatcatgc
     7441 gaattggact cgtcgcaaca gtccttcatc gagttgaacc tatatctatg tgttgttaat
     7501 aaatagcgcc cctcttagtg cagtttgcat aaggaggggc gctgtgtata cgtgggagga
     7561 gttagatgct ctgcccttta tttgatttag cctctcatgc ggcggaaagc ttcatgtgca
     7621 gcttcaatcg tacgctcgat gtcttccgcg gtatgctcgg tagtcaggaa ccatgcttcg
     7681 tacttcgaag gcgcaagaca gatgccttga tcgagcatat gacggaagaa tgcgccgaac
     7741 gcttcggtat ccgtctcttg tgcttctgca taattcgtga ctggatgatc gcagaagtgc
     7801 gtcgagaagg agccgcgaat gcggttgatc gtaagcggca cgccataacg gtcagcgcct
     7861 tcttgaatgc cgttcgtcaa gcggattgcc agctcctcca tttgctcgta tacgccctct
     7921 gcttgcagaa cctccaagca cgcaatgccg gctgagattg aagcagggtt gcctgccatt
     7981 gtgcctgctt ggtatgcagg gccgagcgga gcaacttgtt ccatgatgtg cttgcgtccg
     8041 ccataggcac cgatcggcag gccgccgcca atgattttgc ccatcgctgt caagtctggc
     8101 tcaatggctg caaagacagg gaaggcgcgg taagtctgcg ccgaaccata atggaatcgg
     8161 aatgcgctga tgacctcatc atagataacg agcgccccgt attcgcgagc gagcttgcac
     8221 aagccttcaa ggaagccttc tgcaggcata accatgccga agttgccgac gattggctca
     8281 accataacgg ctgccacatc gtctccccaa tgctccagtg cttccttcaa gctgtccaga
     8341 tcgttgaatg gaacggtgat tacttcgtca gcaatgctgg atggaatccc tgcgctgtcc
     8401 ggaataccaa gcgtagaagg gccagaacca gctgcaacga gcacgagatc cgaatgccca
     8461 tggtagcatc cggcgaattt gataaccttc gtgcgcttcg tataagcgcg ggctacgcga
     8521 attgtcgtca taacagcttc ggtacctgag ttgacgaagc gtactttatc catagacgga
     8581 acagcttcct tcaacatgcg agccagcttg atttccagct cggtcggcgt tccgtacaag
     8641 gtaccgttct gcgcagcgcg aacgattgcc ttcgtaacat gggggtgggc atgacctgta
     8701 atgatagggc cataagcagc caagtagtca acatagcggt tgccgtctac gtcccagaag
     8761 taagcgcctt gtgcgcgttc catgaagacg ggggcaccgc cgccgaccgc tttgaatgag
     8821 cgggaagggc tgttgacccc accgacgata tgttgaagcg cttcttcgta cagttgtgca
     8881 gaacggttgc gattcgtagt catgtagatg aatccccttt attgtgaatt agtttcagat
     8941 acggattgcg tgacattcac ttccaaagct ttttgaatgt attgcttcag cttatcgtcg
     9001 tcaatgaccc ggagcacggc agcgttattc actcgtgcat cagtgacatt gtccatcggc
     9061 gggagctgat gactctgccc aatcgagctg tcgtagccaa gcgcggccag cttcacgatg
     9121 tcatcggctg ataagtttgt ctcgacatag ggcgacactt ccctgataag agaggggagc
     9181 tgcagcaggt tccaggttga tttgagcttg ttggctaatg ccccgagcag atcgcgctgc
     9241 cgctgggtgc gagcataatc ggacatcgca tcatagcgga agcgggcgta cataagcgcc
     9301 gtttcgccat ttagcagctg ctttcctttt ttcaaatcaa tatcgtattg atgcttatct
     9361 gccttgctcg tatatttcat atccttctcc acttcaaatt ccacgccgcc cagcgcgtca
     9421 atcaacttga taaatccgac gaagtctgta tacacataat attgaatgtc cagtcctgtc
     9481 aagtcgcttg ctgtcttcat agccagctcc ggtccgccat aggcgagcgc cgcattgaga
     9541 cgatccttgc cgaagccagg gatgtcaacg tatgtatccc gcaagaggga gaacaagttg
     9601 gcgcgcttcg tcttcggatc gaaggaggcg agcagaatag aatctgaccg tgggacttcg
     9661 ttagcctcca gtccgcgaga gtctccgccc agcagtaata tgttgactct ctcttctccc
     9721 tgccatttgg gaggctctat gtcgaccttc tcgggcacgc gatgtacgac gggggtgaag
     9781 cgagaggttt cgggcggctt ctgcagactg tccaagctgc tgtacatgga ccacatataa
     9841 tatccggcac tagccagaag gaatacacaa acagaaataa gggcatgctt ccaatacttt
     9901 ttcaacatat ggatcgtcct ttccgcatcc ggcggcaaag cggcgagagt catgatgtcg
     9961 aatccggtgc attcagcatt ctttcaagtc gacctacgtg ctatagtaaa ggttatgggc
    10021 aaatgctccg catcttgtct aatcaattat agaattttta cagaatcgaa tcaattgaat
    10081 gacttgtacc cgcgtaacgg gtcggtgacc agggaaaggg tgacagaagt atatatgaac
    10141 gctattgacg tgcgggattt acgcaaaacg ttccaggtac agaaaaatcg cgatggactg
    10201 aagggagcac tccttgattt attcaagcgg gaatataacg aagtaatggc agtgaaggat
    10261 atctccttca cgatcccaca aggggaaata tgcggatata ttggggagaa tggtgcaggc
    10321 aagtcaacga caatcaagat gctgaccggg attcttgtgc caacctccgg tgatattcgt
    10381 gtgaacggat ttatcccgca taaggaaaga gagaagttcg tgcagggcat cggcgttgtc
    10441 ttcgggcagc gcagccagct gtggtgggat atcggcgtaa ttgaatcctt ccggctgctg
    10501 cgcaaagtgt atcgcgtctc tgaacatgat ttcaagcagc ggctggatga gctggttgag
    10561 cgtcttgcct tgcaggagct gctgaaccgc ccggtacgga agctgagtct tggacagcgc
    10621 atgcgctgtg aattggtggc atctctgctg caccgtcctt ctgtgctatt tctggatgaa
    10681 ccaacgattg gactcgatat tgtcgtgaag acggaaattc gcgagttcct aaagcagctg
    10741 aatgcagagc atggaacaac gattttgctg actactcacg atttgcagga tatcgaagct
    10801 ctatgtacgc gtgtcattat gctcgatgat ggacgcatta tttatgacgg cggattggac
    10861 acgctcaagt cgacgtgggg caagggacga gaggtccgtt tccaatttgc gaagcctatg
    10921 cgagcaggtg atgttgagga gcttacgtcc ggccttgatg tggaatggac agttgaatct
    10981 ccttatgaag cgggagttct tgtgccattt gccacgaatg tatcggatgt tatagggcga
    11041 gtggtaggcg gtgcgtcgat atcggatttg agcattatcg agacgaatac cgacgatatc
    11101 gttcgtgaga tttacaaggc gggcacggcg gagaagccgg aagggctgag ccctctcttg
    11161 gccgtacagt cgaagatgga agtggagtca tcttcatcag atacagatgt gaatacgggc
    11221 gattccaatc gcaaggaaga gaacgacaag gagaaggaag agaaggaagc ggtgactcat
    11281 ggctagcgca tatcttgaat ttatccgcat tcgcttcctg ttgatgctgg cttaccgtgt
    11341 taattactat tccggcattc tcatctatac gctcaacatt ggcgtgtatt atttcacctg
    11401 gcaagcgatc tacggcggac aaggaacact tgcaggcttt acggctgacc agatgacgac
    11461 ctacatcgcg gtatcttgga tggcgcgcgc cttttacttc aacaacatgg atcgtgatat
    11521 tgccatggaa attcgagatg gcagcgtagc gatccaattt attcggcctt acaattacct
    11581 attcgtcaag ctgatgcagg cgttcggcga aggcttgttc cgattgctgc tcttcatggg
    11641 gccgggtatg gtcatcgcat gcattctttt cccagtgaag cttccgcagg atccgaagca
    11701 gtgggcggta ttcggtgtca tgctgtttat gagcttcctg attaattcac agttgaatat
    11761 gctgacggga ttgacggcct ttttcatcga aaataatgag ggcatgattc gtatgaagcg
    11821 ggttgtcgtc gatttgttct ccggtgttat tgtaccgatc agcttcttcc caggctggct
    11881 gctcgtgttg aatgaatggc tgccattcca agcgatcaca tatttgccga gctctgtatt
    11941 tacaggcaga gtggtgggcg atcaagtgtg ggcggcgctc ggcatccaat tgctgtgggc
    12001 ggtagtgctt gttattccaa ttgtgctgct gtggcgttcc gcgcgcagac gcttgttcgt
    12061 gcagggaggt tgatcacgat gtattacttc ggacttgtta gtgagtattt gaaaaactat
    12121 attaaaacac gccttaccta tcgtgcagac ttctggatag agatcgcatc ggatc
//
