LOCUS       NZ_JAPDMW010000005     11956 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Paenibacillus alvei strain LF-11/2021
            NODE_5_length_505835_cov_30.377313, whole genome shotgun sequence.
ACCESSION   NZ_JAPDMW010000005
VERSION     NZ_JAPDMW010000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus alvei
  ORGANISM  Paenibacillus alvei
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 505835)
  AUTHORS   Fallico,L.
  TITLE     Analysis of the Paenibacillus alvei's inhibition ability against
            multidrug resistant bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 505835)
  AUTHORS   Fallico,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) U.O.C. Microbiologia, Ospedale San Bortolo
            AULSS8 BERICA, Viale F. Rodolfi, 37, Vicenza, Vicenza 36100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 71.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 02:12:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,998
            CDSs (total)                      :: 5,902
            Genes (coding)                    :: 5,824
            CDSs (with protein)               :: 5,824
            Genes (RNA)                       :: 96
            rRNAs                             :: 9, 1, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 1, 8 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 65 of 78
            Pseudo Genes (internal stop)      :: 14 of 78
            Pseudo Genes (multiple problems)  :: 27 of 78
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:37:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 215367
            Orig. end                         :: 227323
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPDMW010000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11956
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11956
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11956
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6956](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6956
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(80..93)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.93"
                     /tool="antismash"
     gene            145..579
                     /locus_tag="OF778_RS12395"
     CDS             145..579
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549772.1"
                     /locus_tag="OF778_RS12395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_268624704.1"
                     /transl_table=11
                     /translation="MNNMNTPVNLKGDSDWEQVRELLTACMYPNEEKINSQVVLYRSDE
                     SLLLYGVVIEAELVGLIGVRADATGEGVLRHIAVKPHLRGQGIGHAMIKHVMQIHNLVR
                     LEAETDRDAVGFYQRAGFAVTSLGEKYPGVERFLCVLKSY"
     misc_feature    complement(168..183)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 21.07"
                     /tool="antismash"
     gene            703..1572
                     /locus_tag="OF778_RS12400"
     CDS             703..1572
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675090.1"
                     /locus_tag="OF778_RS12400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_268624705.1"
                     /transl_table=11
                     /translation="MPNQTLAVEQLRITDTQGLIDLSASIGWDYDEQDIQTIMSSGTVW
                     GHRNEQGDIISSAAILTYKPGLSTIGMVIVDQGYRGMGLGKAVVQACMDAVDNSDTIML
                     IATEEGRPLYEGMGYHIVSYVNKLVCSSYNTLSNRRDSVSEMRTMPLGLEHFPGIRELD
                     QLAVGADRETFLSARWKQAKQGVVVLDNKGTLIGYGFAVQGPKYLVFGPVVAVQQEAAS
                     AMIHYLAEDNRGKLLRIDVPEEQPEFIELLIQAGFERETHPPVMIKNAQQLPARNRTLY
                     GLAAQIFG"
     gene            1815..2369
                     /locus_tag="OF778_RS12405"
     CDS             1815..2369
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736622.1"
                     /locus_tag="OF778_RS12405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family protein"
                     /protein_id="WP_326848320.1"
                     /transl_table=11
                     /translation="MISTFPRLETDRMILRQLRSEDAPNLFENFSTDEVTQYYNLDNFV
                     AIEQAEQLIERWNQRFEEGQSIRWGIALRSEDRIIGTCGYHNWRRADCKAEIGYELNPN
                     HWSKGLMTEALNRIVAFGFEEMQLNRIEAYVFETNTASQKLLEKSGFHQEGLLQECYRK
                     NDSFIDAILFAKLQKDYTENG"
     gene            2483..3217
                     /locus_tag="OF778_RS12410"
     CDS             2483..3217
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF778_RS12410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_268624706.1"
                     /transl_table=11
                     /translation="MKKIISIVVVVLLLSFVFFVYTEMEGLPWKHAEVKRKAIDYMKQK
                     YNMDVAAAGSSYNFKFKVYTAKVYNVKDSNKAIIRVDDDSDFDAEGNYVGKRLQDDYTL
                     VYWSESIRDGLQVKHPDLFKLQDIDTIQIEYAYYTLSLTEGLSGDQDQNGVHIPVKPKD
                     VPELFIRLKSVDIGDHVLEQLREVINDMVRTSQPMGVFVRAAETKGTQQNKMSKNEILH
                     LEHDQLKFIQSIEDLKKDIKLL"
     gene            3424..5004
                     /locus_tag="OF778_RS12415"
     CDS             3424..5004
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF778_RS12415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_268624707.1"
                     /transl_table=11
                     /translation="MSKQKQGVLAIGSGSILDSLVKTWHEAGDFTLTVCTTGTESPDHV
                     AFTVHQEPDEQGRLTSNVTMNTERRDLKQDWCRIIRPYRFILYAAQLENLAEWRDLQDA
                     CELEGKPLLPAITLRGVGLVGPLLDVDGSARPEAVWRNLHPTVFRSENEQLSFSLTALH
                     ILTNLIVHEYHREVSAGREGNCRDQCYILDPNSLTGSWHSIRSHLFDVERGSKAACRIS
                     AVEEVITASNVRMNESDWYTSFADMTSSSIGIFHKWEEAGIVQLPLAQCMVQPIDPASE
                     SFVRLLPAIMCSGLTHEEARRESGLAGLEAYAARLLPFLFPVSGNDHVADISVGAGCEA
                     AEAVGRGIRACLTKRLQDRIDRSRHGTSQMIVSRLRYEHVADSRCEYYLQSLRIMGNES
                     IIAVGEPLLGYPVVWVYSRDNWYGSVDVDFTIALSHSLQKALNQVEGHTSDLFLVQCEE
                     DNSMNTVVIPHIPYPFTPAYWIATVQRVLEKNKKDDSCFEIYDLHSSSFLSTEAFFVCG
                     VRLSEEGST"
     gene            5001..6956
                     /locus_tag="OF778_RS12420"
     CDS             5001..6956
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549760.1"
                     /locus_tag="OF778_RS12420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="WP_268624708.1"
                     /sec_met_domain="YcaO (E-value: 1.2e-77, bitscore: 256.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 1.9e-121, bitscore:
                     400.1, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTVLVIGEGLLADVVYAQLTDQCNYNVARLTNHNEVIPSTSISI
                     ALVLQDQENLGLLLDVNEKLQQGGIPWISAYLSGEEGVVGPMNWPGASGCFQCADIRMS
                     IAGSNTNATDAILMKLVNPDYVPRDEPDIEVTGYRYMAQLLVEETSKIVNGHSLCTDSH
                     IYMINLHSLNCTRHYILPYGACPVCGQLEEDSSEAASIVLKPSPKLDNSYRCRSMNELQ
                     GVLFNDYWDRRSGLFNDIELHLSSVFADAVVNLPLGYYDEVTAGRSHSFTNSQLAAILE
                     GLERYCGITPRGKRPVVFDSYNQLMEVAMDPATIGLHAQEQYEQEGFPFASFDPDTPIE
                     WVWGYSFLQERPILVPHLVAYYSLGHEGSFVYETSNGCALGGSLEEAVLYGIFEVVERD
                     AFLMTWYAKLQRPRLDPYSSGDEELLLMIQRIKAVLGYEVLLYNVTTENGIPSIWAIAK
                     GAAEHEVSVVCSAGAHLDPIRAAKSAVHELAGTISMVEKRWKERGSEALSMFMDSYEVQ
                     QMEDHTLLYSLPEAAERLHFLLEEQRPVRSFPEEFPTPVVPVSTDVTDELKEVLEVFRR
                     LQLDVIVVDQSSSETLRNGLHCVKVLIPGMLPMTFGHHLRRLEGIDRVLEVPMKLGYVD
                     RKLTREELNPYPHPFP"
     gene            7230..7853
                     /gene="yqeK"
                     /locus_tag="OF778_RS12425"
     CDS             7230..7853
                     /EC_number="3.6.1.41"
                     /codon_start=1
                     /gene="yqeK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007127899.1"
                     /locus_tag="OF778_RS12425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="WP_268624709.1"
                     /transl_table=11
                     /translation="MHLLFEKLVQEIEFTGNMKQDIQQFLTENHSPGTAEHCMDVGTEA
                     RRIAQLYYADPEKAELAGWLHDISAVFPNEERIEAARQLGIDILPEEEQFPMIIHQKLS
                     KVMARDIFGVQDAEVLDAVGCHTTLRADASQLDLVLFVADKIAWDQQGIPPYIEELNHN
                     LAISLEHAAFAYIQYLWERKDTLKVIHPWLADAYEQLHVQLNAE"
     gene            8114..9430
                     /locus_tag="OF778_RS12430"
     CDS             8114..9430
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF778_RS12430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="J domain-containing protein"
                     /protein_id="WP_268624710.1"
                     /transl_table=11
                     /translation="MNVWDVLGIEPTEDDSVISSAYAKKLTLEHPENDPQWKEMLHEAY
                     EVALKLARKRSAATEASNMRLNDHGERLEDPFVHKTIAREEVAVSTAYPYAANPIQVFL
                     GRVSELYSNFANRIQSSSWKVLFQENAEWDIENPQLRDEMVMFLEEKHYFPMAVWAVLE
                     KTFRFLEHEEELRERHAGEIIDYIFEQVNGTMELTYDCFIDADIDFDIDRYLYLREMAQ
                     YMLMQGELEEVGTYLSEAHEWFQDDTDLELMRGKYFDAIGKLEQAMDCFNQVIAMKPDE
                     FEGRWHRAQLLYKLEQYEAALADCNRLLQIEENQQDALMLSIHCYTQLGHLKEARMKLQ
                     QSNDIDNIHDILRYVHGIKLFNKYRYAQEVEEKGQRQARKQISSFLLYFYLRLTWLYLL
                     IYVGLQLFISVPLVVTVVWLAVMVWNGWKTFKTYRIMTT"
     gene            9446..10108
                     /locus_tag="OF778_RS12435"
     CDS             9446..10108
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018584.4"
                     /locus_tag="OF778_RS12435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1266 domain-containing protein"
                     /protein_id="WP_268624711.1"
                     /transl_table=11
                     /translation="MPSEYNKQQRKQLELYFRCLSSVCTDSVMGYHYIPFGFPFTDRIL
                     RRSSMKKAFSAWSVTDKESLLSRVQWLLEDGLREEYKKLHMSLAARNAVSRKRFVESSV
                     DHPDHAKVIIVNAFLHQLPSGEIAAFGGAWAIYMCNMGITYGYLTKDEAWGLKLKSAHH
                     LQNCYKSWEDYLAAFAAGNSYFASDSEMKMQTHITSRASLLLGSSTLVMDKAKWGLA"
     gene            10144..11193
                     /locus_tag="OF778_RS12440"
     CDS             10144..11193
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679490.1"
                     /locus_tag="OF778_RS12440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_268624712.1"
                     /transl_table=11
                     /translation="MAAEVHVASIHDYFERYSEMIHGDMESSTWEQRVFLPPHIGTGSV
                     TRMRVREGMEIMVTDVTYAEDMRLHIQEACRLFELSYCLSGEVYCEWGGKASHTEPQSG
                     NVLLLEDVQVYEEKKAGMHHHMVEVRFSPDELYAYAADAAEKQNMERLLHRHLGSIDSY
                     SVTPDIHRCVTDMLRCDYRGKMKRLYMESKAIEFLALFGETDEIHDRTVRRNVRRDDIA
                     KLHEAREIVMNNYEQPLSIRELSKHVGINEFKLKAGFRELFGSTVFELVRKQRIEKAIW
                     HMEMGHLNVGEAAVAVGYSNVSNFTAAFRKHYGCNPSSFLKQLEQQDQVRRSSSNGGDS
                     SISQGDPLA"
     misc_feature    complement(11613..11628)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
ORIGIN
        1 atggggttat tttatcgttt tggcagttct cgttggcggc ggcattggaa tgtacgcatg
       61 gtttaaaaga aaaggctggt ttgattgaaa aatggagcag ggaattcaag aaaataagag
      121 tcgctatatg gaggccgcaa caacatgaac aacatgaata caccagttaa cctcaagggg
      181 gattcggatt gggagcaggt gcgagagttg cttacagcat gtatgtaccc gaacgaagag
      241 aagatcaata gccaggttgt tctctaccga agcgatgagt ccctgttact gtatggagtc
      301 gtcattgaag cagaactggt cggattaatt ggtgtacgtg cagatgcaac cggagaaggc
      361 gtactgcgtc atatcgcggt aaagccgcac cttcgtggtc aaggaattgg acatgcgatg
      421 atcaaacatg tgatgcagat ccacaatctt gttagacttg aagcagaaac ggatcgagat
      481 gcggttggtt tctatcagag agctggcttt gctgttacaa gtctgggtga aaaatatcca
      541 ggtgtggagc gatttttatg cgtgttaaag agttattagg tgtataataa tggaataatt
      601 gaatttcgac ctcagaccca gtcctacaga agcatgatct tgtaaagctg ggttctttta
      661 tttctatttc taaagtgtgt gctcagaaag gtgatagatt tgatgccgaa tcagacatta
      721 gctgtagagc aattgcgtat aacagatacg cagggattga tagacctatc cgcttcgatc
      781 ggttgggatt atgatgaaca ggatattcag acgattatgt catcgggaac ggtatgggga
      841 catcgcaatg agcaaggcga cattatttca agtgctgcta ttcttaccta caagcctgga
      901 ttgtctacca ttggtatggt tatcgttgat caaggctata gagggatggg cttagggaag
      961 gcggttgttc aagcttgtat ggacgctgtt gataacagcg acaccattat gctgattgca
     1021 acggaagaag gccgaccttt gtatgaaggc atggggtatc atatcgtatc gtacgtcaat
     1081 aagcttgtat gttctagcta caacacatta tctaatcggc gtgattctgt ctctgaaatg
     1141 cgtaccatgc cgctagggtt ggaacacttc cctgggattc gtgaactgga tcagttggcc
     1201 gttggagcag acagagagac attcctatct gctcgctgga aacaggcgaa gcagggggta
     1261 gttgtactgg ataacaaagg tactctgatc gggtatggat ttgccgttca aggcccgaag
     1321 tatctcgttt ttggaccagt ggtagctgta cagcaggaag cggcatcagc catgattcat
     1381 tacttggccg aagataaccg gggcaagctg cttcgtattg atgtgccaga ggaacaacct
     1441 gaatttatcg agcttttgat acaggctggt tttgagagag agactcatcc tccggtgatg
     1501 attaagaatg cacaacagct gccggcaaga aatcgtacac tatacgggct tgcagcgcaa
     1561 atatttggat aacgggtgga actaggtcag aagtgtcttg cccatacgcg tgtagctggt
     1621 caacgacaat gtaaactgag gtgaaatgaa tatggagtgg tctgctctta ttatctcgtt
     1681 tttcttctta gctacactcg tcagaattga aattaacacc aagaagaagc ttgataatga
     1741 tatgaagata atgaaacagc ttcagaatat cttggatgaa ttaaagaaca tgaagaattg
     1801 aaggaggaag caagatgata tcaacatttc ctagattaga aacggacaga atgattctac
     1861 ggcagctcag gtcggaggat gcgcccaatc tattcgagaa cttctcaacg gacgaagtaa
     1921 cgcagtatta taacttggat aatttcgtgg cgattgaaca agccgaacaa ttaatagaga
     1981 gatggaatca acgatttgag gaaggacaga gcattcgctg gggaattgca cttcgatcgg
     2041 aggatagaat cattgggact tgcggctatc acaactggcg gagagcagat tgcaaagccg
     2101 agatcggata cgaattgaat ccaaaccatt ggagtaaggg cctgatgacg gaagcattga
     2161 accgcatagt cgcatttgga tttgaagaaa tgcaattaaa tcgaatcgaa gcctatgtgt
     2221 ttgagacaaa tacggcttcc caaaaactgc ttgagaagtc cggattccac caagaaggat
     2281 tgctgcagga gtgctatcgc aaaaatgaca gttttataga tgctattcta tttgccaagc
     2341 ttcaaaagga ttatacagaa aacggataaa tgatgaagtg aaactgtgca ctccgcactc
     2401 gcatgttgta cacgaatcag ttacgcggag aagtgagacc aatgaagtta gtgcgtataa
     2461 ttcggtcagg taggtgttgt gcatgaagaa aataatatcg attgtggttg ttgtcttgct
     2521 gttgtctttt gtgtttttcg tctatacgga aatggaagga ttaccttgga aacatgctga
     2581 agtaaagcgg aaggcaatcg attatatgaa acaaaagtac aatatggatg ttgcagcggc
     2641 aggaagttct tataatttta aatttaaagt atatacagcg aaagtatata acgtgaagga
     2701 ttcgaacaaa gcgattattc gtgtggacga tgatagtgac ttcgacgccg agggcaacta
     2761 tgttgggaag cgattgcaag acgattatac ccttgtgtat tggtctgaat ctattcgcga
     2821 tggactgcaa gtaaagcatc ctgatctgtt caagcttcaa gatattgata cgattcagat
     2881 tgaatacgca tattacacgt tatcacttac cgaagggctc agcggtgatc aggatcagaa
     2941 cggggtgcac attccggtaa agcccaagga tgttcctgaa ttgtttattc gattgaagtc
     3001 tgtcgatata ggcgaccatg tgttggagca attgcgtgag gtaatcaatg atatggtgag
     3061 aacctcccaa cccatgggtg tcttcgtaag ggcagctgag acgaaaggga ctcaacaaaa
     3121 taagatgtca aagaacgaaa ttcttcatct agagcacgat caactcaagt ttatccaatc
     3181 tattgaggat ttgaagaagg acataaagct cttgtaaggg aaggcagagc tgtctcaact
     3241 gtcattacgc taatgacatg aggcagctct ttttttttgt tgcagtagga cagggccgta
     3301 cgcgagtcat agcgaagcgg tcgtcgacat acgttaacaa tgacatttct aggtcggatt
     3361 cacgataacg aagcatatcg aattctatga aatacaggcg ttacgggaaa ggaggattgt
     3421 tatatgtcta agcaaaaaca gggagtactt gctatcggct ctggatccat tctggattct
     3481 ttggtaaaaa cctggcatga agcgggagac ttcacactga cggtttgcac aactggcacg
     3541 gaatcccccg atcatgttgc gttcacggta catcaggagc ctgacgaaca gggaagattg
     3601 acctcaaatg tcacgatgaa tactgaacgt agagatctca aacaagattg gtgccgtatt
     3661 attcgaccat accgttttat cctatacgct gctcagcttg agaatttggc tgaatggaga
     3721 gaccttcagg atgcatgtga attggagggg aaacctctac tgcctgcgat aaccttacgt
     3781 ggggttggat tagttggacc attgttagat gtggacggat cagcacgacc agaagcggtg
     3841 tggcggaacc tgcatccgac cgtcttccga agtgagaatg agcagctgag cttctcgtta
     3901 acggcacttc atattcttac aaatctgatt gtgcatgaat atcatcggga agtgtcagca
     3961 ggaagagaag ggaattgtag agatcaatgc tatattcttg atccgaacag cttaacaggg
     4021 agctggcatt ccattcgttc acatctcttc gatgttgaac gagggagtaa ggcggcttgc
     4081 cgtatatctg cagtggaaga agtcatcaca gcaagtaatg tgcgaatgaa tgaatcagat
     4141 tggtatacca gcttcgccga tatgacttcc tcttcaattg gaatttttca caaatgggaa
     4201 gaggcgggaa ttgtgcagct tcctctcgca caatgcatgg tgcagccgat tgatccggca
     4261 tctgaatcat ttgtccgact actgccagca atcatgtgca gtggattgac gcatgaagag
     4321 gctcggcgcg agtcgggact tgcaggattg gaagcgtatg cggcacgttt attaccgttt
     4381 ctgttccctg tatcggggaa tgaccatgtg gctgatatca gtgtaggtgc aggttgtgag
     4441 gcggctgaag ctgttggacg gggaattagg gcgtgcttga cgaaacgatt gcaggatcgt
     4501 attgaccgca gccgacacgg aacgagtcaa atgattgtat cgcgactgcg atatgaacat
     4561 gtagcagaca gccgttgtga gtattatttg caatcgctac gtatcatggg aaatgagtct
     4621 attattgcgg tgggggagcc gctgctaggt tatccggtag tatgggtcta ttcgagggat
     4681 aattggtatg gaagtgtgga tgtagatttc acaattgcgc tgagccattc ccttcagaag
     4741 gcgctaaatc aggttgaggg gcatacgtcc gatctatttc tagttcaatg cgaagaagat
     4801 aattcaatga acacggtcgt gatccctcat attccctatc cgttcactcc tgcttattgg
     4861 atagcaacgg tgcaaagagt attggagaag aacaagaagg acgattcgtg cttcgaaata
     4921 tatgatttgc acagcagttc tttcctgagt acagaggcat tcttcgtatg tggagtgcgg
     4981 ctcagtgagg agggctctac gtgaacactg tcctggtgat tggagaggga ttacttgcgg
     5041 atgtcgtata tgcacagtta acggatcagt gtaattacaa cgtagcaaga ttgacaaatc
     5101 acaatgaagt aattccaagt acaagtatta gcatcgcatt ggtgcttcag gatcaagaaa
     5161 accttggatt gctgttggat gtgaatgaga agcttcagca aggtggaatt ccgtggatat
     5221 cggcttattt gagtggggaa gaaggggtcg tcggtccgat gaattggcca ggtgcatccg
     5281 gatgcttcca atgcgcagac attagaatgt cgatagcggg cagcaacact aatgcaactg
     5341 atgccatact tatgaagttg gttaaccctg actatgtccc acgagatgaa ccggacattg
     5401 aagtgacggg gtatcgatat atggctcagt tacttgttga ggagacatcg aaaatcgtaa
     5461 acggacatag tctgtgtacc gacagtcaca tttatatgat caacttgcac agcttgaact
     5521 gtacacggca ttatattctt ccttatggtg cttgtccagt atgcggccag ctagaagaag
     5581 attcgtcgga agcagcgagt attgtgctta aaccatctcc caagctggat aatagttatc
     5641 ggtgtcgttc tatgaatgaa ctacaggggg ttctgttcaa cgactattgg gatcgtcgat
     5701 ccggcttatt taacgacata gagctgcatc tttcttctgt atttgcagat gcagtcgtca
     5761 atctcccact cggctattac gatgaggtga cagcgggacg ttcccattcg ttcacgaaca
     5821 gtcagcttgc tgccatatta gagggattgg aacgttattg cggcatcaca ccccgcggta
     5881 aacgtccagt tgtgttcgat agttacaacc aactgatgga agttgctatg gatcccgcta
     5941 caatagggct tcatgcacaa gaacaatacg aacaggaagg ttttccgttt gcctcctttg
     6001 atccagatac ccccattgaa tgggtgtggg gttattcatt tttacaggag cgcccgattc
     6061 tagttcctca tctagtagcc tattacagct tagggcacga aggaagtttc gtatatgaaa
     6121 cctctaacgg ctgcgcatta ggaggcagtc tggaagaagc ggtgttgtat ggcatatttg
     6181 aagtagttga acgtgatgcc ttcttaatga cttggtatgc gaagcttcaa cgcccaaggc
     6241 tcgatccata ttcctcaggg gatgaagagt tgctcctcat gattcaacgg atcaaggctg
     6301 tcttaggata tgaggtgtta ttgtacaacg taacaacgga gaatgggatt ccaagcatct
     6361 gggcgattgc taagggggca gcagaacatg aagtgagtgt agtctgttct gcaggagctc
     6421 atctagaccc gatacgagca gctaagagtg ctgtccatga acttgcgggg acgatctcca
     6481 tggttgaaaa acgttggaag gaacgtggtt cggaagcgct gtctatgttt atggattcgt
     6541 atgaggtaca gcagatggaa gaccatactc tgctgtacag tttgccagaa gcagcggaaa
     6601 gacttcactt tctattagag gagcagcgtc ctgtccgttc ctttcccgaa gaattcccta
     6661 ccccagtagt tccggtaagt acagatgtaa cagatgagtt gaaggaagtt cttgaagtgt
     6721 ttcgtagatt gcaattagac gtgattgttg ttgatcaatc gtccagcgag acgctacgca
     6781 atggtctaca ctgcgtgaaa gtgctgattc caggaatgct tccaatgact tttggccacc
     6841 atctacgtcg attagagggg atcgacagag tgttggaagt accgatgaaa cttggatatg
     6901 tggatcgcaa gcttacacgt gaggaactga acccttatcc gcacccattt ccgtaaggga
     6961 tgttgactga acggcattcg gattgactcc aatgattagc atgattcatg cataaggacg
     7021 aaaacaaggt ggtattgaaa taaacatact ggtaaaataa gtgaatgaga agttgattcc
     7081 atgaaacagc gattgggata tgttgagctc aatcgctgtt ttgatctatt tctaaatgct
     7141 atctgataag aagccgacca tttctttttc aaaatagaaa taaatgatta atagggatga
     7201 cattccatag tgagagaggt atatctatca tgcatctatt atttgaaaag cttgtgcagg
     7261 aaattgagtt cacgggaaat atgaagcagg atattcaaca gtttctaacc gaaaatcata
     7321 gcccgggcac tgcagagcat tgtatggatg tagggacaga agctagaaga attgcgcaat
     7381 tatattatgc tgatcctgag aaagcggaat tggcgggatg gctgcatgat ataagcgccg
     7441 tatttcctaa cgaggaacgc attgaggcgg cgaggcagct aggaattgat atactgcccg
     7501 aagaagagca attccctatg atcatccatc agaagctatc caaggtgatg gcccgggaca
     7561 tatttggcgt tcaggatgcc gaagtgctgg atgcggtggg ctgccatact acgctgcgag
     7621 ccgatgcctc gcagcttgat cttgtactat ttgttgccga caagattgca tgggatcagc
     7681 aaggcattcc tccttatatc gaggaattaa atcataatct cgcaatatcg ctggaacatg
     7741 cggcgtttgc ttacattcaa tatttatggg agcgcaagga cacactgaag gtcatccacc
     7801 cttggcttgc agatgcttat gaacaattac atgtacaatt gaatgcggaa tgaagttgca
     7861 ttgtggagtg gtctgacgca gtaggcgtca gaccactcca tttttcgttg gctgctatcg
     7921 gaaatttgaa agagaaaggg ggctcccact tagttcgaat ctagtataaa ttggattatc
     7981 tcttgtggta ttggtagaat aaagaatggt tgaaggaagt ttttcgattt tactattgac
     8041 tatcggaata ttgaaatagc caccaaagca tattgggaac ggctggagtt gagggaaagg
     8101 tggatggaat aacttgaatg tatgggatgt attagggatt gagccaacgg aagatgattc
     8161 tgtcatctca agcgcttatg cgaagaagct gacgcttgag catccggaaa atgacccaca
     8221 gtggaaagaa atgctgcatg aagcgtatga agtagcgttg aagctggcgc gcaaaaggtc
     8281 agcagcaaca gaagcttcta acatgaggct gaacgatcat ggtgaacggt tggaagatcc
     8341 atttgtacat aagacgattg caagagaaga ggttgctgtg agcacagctt atccgtatgc
     8401 cgcaaatccg attcaggtat ttctaggccg agtatctgaa ctgtatagca acttcgcaaa
     8461 tcgcattcag tcttcatctt ggaaagtctt gttccaggag aacgcggaat gggatattga
     8521 gaatccgcag ttacgtgatg aaatggtgat gtttcttgag gagaagcatt attttccgat
     8581 ggcggtatgg gccgtgctgg agaagacttt ccgttttcta gagcatgagg aagaattaag
     8641 ggaacgccat gccggggaaa tcattgatta catattcgaa caggttaacg gtacaatgga
     8701 gctgacctat gattgtttca ttgatgcgga catcgatttt gatattgacc gttatctcta
     8761 cctgcgtgag atggcgcagt atatgcttat gcaaggagaa ctggaagagg taggcacata
     8821 tttgagtgag gcccatgaat ggttccaaga cgatactgac ctagagctca tgcgcggcaa
     8881 atacttcgat gcaatcggca agttggagca agcaatggat tgcttcaatc aagtgattgc
     8941 gatgaaaccc gatgagtttg agggaagatg gcaccgtgca cagctcctat acaagcttga
     9001 acaatatgaa gctgccttgg cggactgcaa ccgcttgctg cagattgagg agaatcagca
     9061 agatgcgttg atgctgtcca ttcattgtta tacgcaatta gggcacctga aggaagcgcg
     9121 tatgaagctc cagcaatcca atgacattga caatattcat gacattttac ggtatgttca
     9181 cggtattaag ttatttaaca aatatagata tgcccaagaa gtggaagaaa agggacagag
     9241 acaagcacgc aaacaaattt catcatttct cctctacttc tatctccgct tgacttggct
     9301 atacctactc atctatgtcg ggcttcaact atttataagc gtgcctcttg tcgttacggt
     9361 agtgtggctt gcagtaatgg tgtggaacgg ttggaagacg ttcaagactt accgaattat
     9421 gactacctag aaggagggct ccaatatgcc aagcgaatac aataagcaac aaagaaagca
     9481 actggagctg tacttccgtt gtctttcttc tgtatgtacg gacagtgtga tggggtatca
     9541 ttatattccg tttggttttc catttacgga tcgaattctg cgcagaagct cgatgaagaa
     9601 ggcgttttcc gcttggagcg ttacagataa ggaatcattg ctcagtcgag ttcagtggct
     9661 gctcgaagat ggactgcgag aggagtacaa gaagctgcat atgagtcttg ctgcacgcaa
     9721 tgcagtctct cgcaagcgtt ttgtggaatc ctctgttgat catcctgacc atgccaaggt
     9781 cataattgtc aatgcatttt tgcaccagct tccttccggg gaaattgcgg catttggcgg
     9841 ggcttgggca atctatatgt gcaatatggg aattacctac ggctacttaa ccaaggatga
     9901 agcctggggg ttgaagctta agtcggcaca ccatttacag aactgctaca agagctggga
     9961 agactatctt gccgcttttg ctgcaggcaa tagctacttt gcctcggata gtgaaatgaa
    10021 gatgcagact cacattacga gtagagcctc gctgctgctt ggatcgagca cattggtcat
    10081 ggataaggcg aagtgggggt tagcttgacg atttactagc aaggtggaat agaaggtgat
    10141 cagatggcag ctgaggttca tgtagccagc atccatgatt attttgagcg ttattctgaa
    10201 atgattcatg gtgatatgga aagcagcact tgggagcagc gagtgttcct tcctcctcac
    10261 ataggaacgg gcagtgtgac ccggatgcgc gtccgtgaag gtatggaaat catggtgacc
    10321 gacgtcactt atgcggaaga tatgaggctc cacattcagg aggcatgccg tctgtttgag
    10381 ctgagttatt gtttgagcgg agaagtgtat tgcgaatggg gtggaaaagc aagccatacg
    10441 gaaccgcagt ctggcaatgt gctgctgttg gaggatgtgc aggtatatga ggagaaaaag
    10501 gcaggaatgc accatcatat ggtcgaggtg agattttcac cggatgaact gtatgcctat
    10561 gccgctgatg cggccgagaa gcagaacatg gagagattgc ttcatcgtca ccttggcagt
    10621 attgactcct attcggtcac acctgacatt catcgatgtg taacggatat gcttcggtgc
    10681 gattatcggg gaaagatgaa gcgactatac atggagagca aagctatcga attcctcgcc
    10741 ttattcggag agacggacga aattcatgat cgaactgtga ggcgaaacgt tcgacgcgat
    10801 gatatcgcga agctgcatga ggcaagggaa attgtaatga acaactatga acagcccttg
    10861 tcgatacgag aactctccaa gcatgtcggg atcaacgaat tcaagctgaa agcaggattt
    10921 cgagaattgt tcggctcaac agtattcgaa ctggtgcgca agcagcggat cgagaaggcg
    10981 atatggcata tggaaatggg gcatctgaat gtaggggaag cagccgtcgc ggtcgggtat
    11041 agtaatgtta gcaactttac ggcagccttt cgcaagcatt acggctgcaa tccaagctcg
    11101 ttcctcaagc agttggaaca gcaggatcaa gttcgaaggt cgtcttcgaa tggcggcgac
    11161 tcttccattt cgcaagggga ccctttggcc taatgtaggt agaagggttc ttcaattatt
    11221 tcctctatag gggcaaagac tccctcttca ggtagagcat gtgaacatga ttggctcata
    11281 attagagtat tcatgtgatt ctaactactg gagggaatcg taatgtctcc tattttgaca
    11341 actacatcac aacacacgga tggtaatcag caaatggagg ggaatccgaa acgatggatt
    11401 gcactcgtta tcgtactact gccgacgctg cttatctcac tgaacactta tatgattcag
    11461 gttgctctgc cgttaatgca gcacagcttg aacgcaagct tcgctgaagc gcagctaatt
    11521 gttacaggtt tctctcttgg cttggcggtg gccttgatca ttagcgggaa gttgggtgac
    11581 atattcggaa gaaaaaggct gcttcttatt ggagtgagtg ggttcacaat catggccatg
    11641 attggcggct tgacctcgga tccttccctg cttattgcca ttcggatcgt tcaaggtttg
    11701 gctgctgcgc tcattcagcc tcaggttctt tctacgatgc aggtcagctt tccgccacga
    11761 gaaaaggcaa tcgctttcag catatatggt gcaatcattg gatttggatt tgcttttgga
    11821 ctcatttttg gggggcttat cgttaactgg aacctgttcg atcttggctg gcggatggtg
    11881 ttctttgtta acgttccatt cggtatgctg gttctgatgc tgctgccaat cattccggaa
    11941 tcgcacgggg agaaac
//
