LOCUS       NZ_JAPDMW010000004     20531 bp    DNA     linear   CON 23-JAN-2024
DEFINITION  Paenibacillus alvei strain LF-11/2021
            NODE_4_length_506830_cov_35.337152, whole genome shotgun sequence.
ACCESSION   NZ_JAPDMW010000004
VERSION     NZ_JAPDMW010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus alvei
  ORGANISM  Paenibacillus alvei
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 506830)
  AUTHORS   Fallico,L.
  TITLE     Analysis of the Paenibacillus alvei's inhibition ability against
            multidrug resistant bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 506830)
  AUTHORS   Fallico,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) U.O.C. Microbiologia, Ospedale San Bortolo
            AULSS8 BERICA, Viale F. Rodolfi, 37, Vicenza, Vicenza 36100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 71.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 02:12:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,998
            CDSs (total)                      :: 5,902
            Genes (coding)                    :: 5,824
            CDSs (with protein)               :: 5,824
            Genes (RNA)                       :: 96
            rRNAs                             :: 9, 1, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 1, 8 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 65 of 78
            Pseudo Genes (internal stop)      :: 14 of 78
            Pseudo Genes (multiple problems)  :: 27 of 78
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:37:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 199841
            Orig. end                         :: 220372
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPDMW010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20531
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20531
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20531
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10531](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10531)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            complement(502..1500)
                     /gene="plsX"
                     /locus_tag="OF778_RS10070"
     CDS             complement(502..1500)
                     /EC_number="2.3.1.274"
                     /GO_function="GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="plsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006288179.1"
                     /locus_tag="OF778_RS10070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="WP_065290312.1"
                     /transl_table=11
                     /translation="MRIAIDAMGGDHAPKSNVEGALAAAKEWSDVELLLVGDEAKLTPY
                     MTTSPSNVRIIHAPDTIEADDEPVKAVRRKKEASMVVAGRMVREGEADCMISAGNTGAL
                     MTTGLLIVGRMNGVERPALAPMIPTMDGRGVLALDLGANMDAKPEHLAQYALMGSIYRE
                     KVHGMAKPRVGLLNVGTEAMKGNELTKQVYPLLAELPIQFVGNVEARDVLESTCDVLVC
                     DGFAGNVMLKSLEGTASSLFGVLKREFTRTWYTKLAAAMLMPALKGLRKTMDYKEHGGA
                     PLLGLNGLVIKAHGSSDARAVHNAVRQARLALQNDLIETISMGVGGTNSGK"
     gene            complement(1484..2104)
                     /gene="fapR"
                     /locus_tag="OF778_RS10075"
     CDS             complement(1484..2104)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="fapR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551881.1"
                     /locus_tag="OF778_RS10075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transcription factor FapR"
                     /protein_id="WP_028531217.1"
                     /transl_table=11
                     /translation="MVRDIERLPKRQRQQQLAAIIEENPFVTDQELTRKLKVSIQTIRL
                     DRMELGIPELRERLKLMAEQSYDQVRSLPLHEVIGDIVDLQLDRSGISIFEIRDEHVFS
                     RTHIARGHHLFAQANSLAVAVINDEIALTSSADIRFLRSVKLGEKCIAKAYVRSISGER
                     SKAKVEVFSYVGEEMVFQGNFVIYRSAGTVISSEGGELHENRH"
     misc_feature    2181..2195
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 19.02"
                     /tool="antismash"
     gene            complement(2371..2544)
                     /gene="rpmF"
                     /locus_tag="OF778_RS10080"
     CDS             complement(2371..2544)
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370634.1"
                     /locus_tag="OF778_RS10080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="50S ribosomal protein L32"
                     /protein_id="WP_019424932.1"
                     /transl_table=11
                     /translation="MAVPQRRTSKTRRDKRRTHFKLSVPGMVKCEQCGELKLAHHVCKV
                     CGTYKAREIIKQ"
     gene            complement(2593..3108)
                     /locus_tag="OF778_RS10085"
     CDS             complement(2593..3108)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006288176.1"
                     /locus_tag="OF778_RS10085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF177 domain-containing protein"
                     /protein_id="WP_021256603.1"
                     /transl_table=11
                     /translation="MEFRFRDIVAGRGETQIDTAFRADEVARERSDVAISEPVHVELSV
                     KAEGDGFARAHGKLTAKMELQCSRCLTSFTETYVIPFDEQFKLKDSTDLPAEGDEEDVI
                     TVADDLVDLKPYVEEALLMNLPYAPLCDEACKGLCPTCGTNRNEHPCGCSTERIDPRLA
                     GLQDFFKK"
     gene            3366..4634
                     /locus_tag="OF778_RS10090"
     CDS             3366..4634
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551873.1"
                     /locus_tag="OF778_RS10090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase"
                     /protein_id="WP_251486803.1"
                     /transl_table=11
                     /translation="MRTLGIVVEYNPMHNGHVYHLQQSKKVSNCDAVVAVMSGHFLQRG
                     EPALTDKWSRTDMALANGVDLVLELPVAYAVQPAEWFAYGAVATLQATGVVDALCFGSE
                     LGDVEPFLRAADILVNEPSGFTDQLQEHLKAGLTYPAAYAAAAGQAMTASRTSPSSSEA
                     ADWMAQPNNSLGLHYVMALRRLNSAIKPHTIARTQAGYHDTEAPEGSIASATAIRRLWT
                     EEGTLETCAQYLPPSTLRILEQEHRFGRAPMSWDCYTRELIYRLHQLSPESIARHLEVT
                     EGLEYRVKQALGQMTAQRVESLLEQLKTKRYTRTKLQRMLAHILLHHDKFHFGSDGLKK
                     GPWYIRVLGFTDTGRALLKRMKQSAELPIVLSVNRDNFNLGGYCGLEADIRATSLYANA
                     FGRWNARDAYRDYYESPRRHQSN"
     gene            complement(4855..5928)
                     /locus_tag="OF778_RS10095"
     CDS             complement(4855..5928)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551871.1"
                     /locus_tag="OF778_RS10095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PDZ domain-containing protein"
                     /protein_id="WP_174807733.1"
                     /transl_table=11
                     /translation="MEEEAVRDSQGSKRWRLGSWKWFFAALLFLYVMVYMPTPYVIYTP
                     GSAEQVKPMVSVQAGDDTEEGTFMLTTVRRTYANLALLLWKSFDPHAEFGKKADSLQGR
                     SEQEYVTEQLFNMSDSQLGAVLAAYNQLKIPYQLKSEGVYVIYNYPNLAHNEFETNDRI
                     IEVDGKPVNDFEALQAVMKGRKAGETVQVKVERDKKEKLVKATLVELTDPNKKEEKRVG
                     FGLRYGQRKEAIPEDIGKTITFKDSDIGGPSAGLMFTLELINRLTPGDLTEGYRIAGTG
                     TIDPGGNVGVIGGIQFKVVAADREKAALFLAPEGNYAEAKAKLETMNTKMKLVSVRTVG
                     DALNAIQKFGAEQKAAQ"
     misc_feature    6048..6062
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 20.79"
                     /tool="antismash"
     gene            6056..7324
                     /locus_tag="OF778_RS10100"
     CDS             6056..7324
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676616.1"
                     /locus_tag="OF778_RS10100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside recognition domain-containing protein"
                     /protein_id="WP_021256606.1"
                     /transl_table=11
                     /translation="MFSFRTPLSQHPLTTLLMGSMAVLLVICIVSYPDQAFQASLQGLK
                     IWWNIIFPALLPFLVLSEMLYAYGFVHGLGSLLDPLMRLLFRLPGVGGWAWAVGWTAGY
                     PAGAKAVAQLRNQQVLSRQEAERLLCLSHTSSPLFIIAVVGVGFMQKPELGLIIAIIHW
                     ASSILTTLLMRFIHRMRPESQPLRTSASLSKSHQKFYQRIIGQMEGAHRQDGRPFGRML
                     GESVTSAVQTLMMVGGYMMIFSVIIQVLRIAIPQELGKYILNGLLEVNLAAYTLGSAAF
                     SSPVFQAALLSSVIAWSGISALLQVHSIMKTTDLRFGHFILSRLLHALCAFLLTYMLWS
                     PLHSLLQSSDSAMSALCTLATNPSNAYDAPVIAESSSLLPLLTFISHLPIWAQASMLIP
                     VACLFLFTLFILAQTVGRLFTRG"
     gene            complement(7378..7893)
                     /gene="coaD"
                     /locus_tag="OF778_RS10105"
     CDS             complement(7378..7893)
                     /EC_number="2.7.7.3"
                     /GO_function="GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="coaD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CTP_transf_like"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551868.1"
                     /locus_tag="OF778_RS10105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="WP_021256607.1"
                     /sec_met_domain="CTP_transf_like (E-value: 2.8e-23,
                     bitscore: 77.6, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEHTEQVHEPRIAVYPGSFDPVTLGHLDIIQRAAKQFDKLIVAV
                     LNNLSKQPTFSVEERKELLGQITQHIPNVEIDSFRDLLVNYMEQKQGHVIVRGIRSVTD
                     FEYELQMASTNQKLNPRVETIFMMTNPKYSYLSSSMVKEIAKFNGDVSELVAPEVELAL
                     RDKYKDTK"
     gene            complement(7969..8559)
                     /gene="rsmD"
                     /locus_tag="OF778_RS10110"
     CDS             complement(7969..8559)
                     /EC_number="2.1.1.171"
                     /GO_function="GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Methyltransf_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551867.1"
                     /locus_tag="OF778_RS10110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="WP_044353494.1"
                     /sec_met_domain="Methyltransf_3 (E-value: 1.3e-06,
                     bitscore: 22.7, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVISGTARGRSLKPVPGMGTRPTTDKVKEALFSMIGPYFDGGCV
                     LDLFAGTGGLGIEALSRGAEQAIFVDKDPKAVEVVKHNVQAAGVAEQSEVYRNDAKRAI
                     KALTKRDISFELIFLDPPYRLKDADELLEGMWNDGLISKDATIVVEHDSKHEYPEEIGP
                     LYVWKKTEYGEIGITVYRVKEQTEKEAELEQDA"
     gene            complement(8798..9382)
                     /locus_tag="OF778_RS10115"
     CDS             complement(8798..9382)
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /GO_function="GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551866.1"
                     /locus_tag="OF778_RS10115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_021256609.1"
                     /transl_table=11
                     /translation="MRHLIIYAHPNPNSFNHAIMEMVQAELEDQGHAALVRNLYEMEFN
                     PVLSAKDFELFGQHELPPDIEEEQGHITWADHIIMIYPIWWASMPAIMKGYIDRVFSYG
                     FAYKYGADGIEKLLLGKSALLLSTHGNTHEQYEQSGMYEAMQKTTDSGIFEFVGMKVLV
                     HRFFASVTTIDDEARKQMLVSVKELLQKLHR"
     gene            complement(9883..10017)
                     /locus_tag="OF778_RS10120"
     CDS             complement(9883..10017)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF778_RS10120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_265332081.1"
                     /transl_table=11
                     /translation="MKKNIEMFLMKQLEKSAEKEAKKAKIVFGAKKLPTALKNVKHEN"
     gene            complement(10001..10531)
                     /locus_tag="OF778_RS10125"
     CDS             complement(10001..10531)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /locus_tag="OF778_RS10125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_174810877.1"
                     /sec_met_domain="AgrB (E-value: 1.8e-20, bitscore: 67.9,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLEAISRNIAIKLKKNNPESDVDVEVLEYGIAMKLNKYGVILITV
                     TLGTIFGHLYESILALVALAAIRRFSGGAHFSSLTACMIISSLFCVITPMILLSKTTAV
                     VLTVVSVCIYYFRAPNWFEELTVQKNDILYKYIAMLIASGNLILQSSVISIVLFVQALT
                     LLPKGGVKDEKEH"
     gene            complement(10476..11192)
                     /locus_tag="OF778_RS10130"
     CDS             complement(10476..11192)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF778_RS10130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_174810878.1"
                     /transl_table=11
                     /translation="MSQSLTYILFSTLDAAVVNALILKIYKQPLLRYKYKILVLSVALV
                     ICSFLMRTVINLPALDLPLQYIIFVMFYYSVLEYRLHYAAFIIGSGLSAYISIQMIVYY
                     CLAAIGIADKSVIFVYTGFPVNAIQLCSIVVGAAIALFLWMTGRGFSFIIVPPHDTFRI
                     KYSEYSHRIVVISGMISAVTIFLTLLIILSQNYILLMILSLASFGIAYYYSDRGDYESV
                     RSDIEKHCNKIEEEQP"
     misc_feature    11436..11451
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.41"
                     /tool="antismash"
     gene            complement(11476..11916)
                     /locus_tag="OF778_RS10135"
     CDS             complement(11476..11916)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551762.1"
                     /locus_tag="OF778_RS10135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4183 domain-containing protein"
                     /protein_id="WP_268624456.1"
                     /transl_table=11
                     /translation="MNDGNGVIRLTAKPWIIKIEHPYAASSATSSTIMPPQSCASIFPS
                     PPPQPPPPEKDKILKVDTFQYVAICDGLKYEYTNAEAQPQYGSSDILNPLTVSYTNLFL
                     NGMLQPLNVYKVETGLLTLYEVPVEGTPLTLQFVKIYHVEIS"
     gene            complement(12125..12409)
                     /locus_tag="OF778_RS10140"
     CDS             complement(12125..12409)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003342561.1"
                     /locus_tag="OF778_RS10140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4183 domain-containing protein"
                     /protein_id="WP_021262518.1"
                     /transl_table=11
                     /translation="MAIIKPFLASRRFAATLSAGTGTGAAFAVAATSFTNDTGAAATAF
                     PSSFAYYNLYINGVLQTGDTSTITTTNVTIPGGDALDGATPILIEFVIT"
     gene            complement(12730..13191)
                     /locus_tag="OF778_RS10145"
     CDS             complement(12730..13191)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430678.1"
                     /locus_tag="OF778_RS10145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phage holin family protein"
                     /protein_id="WP_142543591.1"
                     /transl_table=11
                     /translation="MDRFNLLLKTGAAAVGGTFSFVFGGWPALVQVLLTMVILDYVTGM
                     LASAVEGKLLSRVGLIGIARKVFIFFIIALAHQIDIAFDSKEMIRDATTYFYMANEVLS
                     IVENGGRLGVPVPNVIKKAIFVLKDKGGNEEGIPKKESKKDDEQEKDEK"
     misc_feature    13365..13374
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 17.75"
                     /tool="antismash"
     gene            complement(13501..14424)
                     /locus_tag="OF778_RS10150"
     CDS             complement(13501..14424)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /locus_tag="OF778_RS10150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="WP_268624569.1"
                     /transl_table=11
                     /translation="MRNKRDTFVQEMESGRNLKVMTFNIWRGSTSIVQVTEVIRTVEAD
                     VIGIQEADGQLPALARKLNYYYDEGHSILSRYPLHQSAHKEFIWIEVTPDEVAAMSNVH
                     LCHEPYGPYDIRDGLDAAAAVANENRIHMQEMNSRFHLLGQLASQGVPVFLTGDFNAPS
                     HLDWTDEAKSMHFGVAVEWPVSIRLEQLQFRDSYREVHSDPLMKPAVTWTPGGNGHIEP
                     DEVHDRIDFVYAGGPSATIDSRIVGERGPYTDIAITPWSSDHRAVVSTFELIPARISMD
                     IGSSIQVSHERIDQGKGTIEASRNIE"
     gene            complement(14534..14776)
                     /locus_tag="OF778_RS10155"
     CDS             complement(14534..14776)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551758.1"
                     /locus_tag="OF778_RS10155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_021256618.1"
                     /transl_table=11
                     /translation="MNITIDLTQIGSAYEHKDWKRVDTIYRHARERMMAGDTVILQRDV
                     PNRAPIAVRTFEQLDELEEWIRHMEDEYCLAEPYA"
     gene            complement(14871..16121)
                     /locus_tag="OF778_RS10160"
     CDS             complement(14871..16121)
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /GO_process="GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1117:
                     sodium/hydrogen exchanger"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129302.1"
                     /locus_tag="OF778_RS10160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cation:proton antiporter"
                     /protein_id="WP_021256619.1"
                     /transl_table=11
                     /translation="MDTIIFEVGLAIALIGLVGVIATKLRFSVIPFYILIGMAVGPHAP
                     HFWIFDFRFIESSVFIDFMGRIGVLFLLFYLGLEFSVGRLLKSGKSIVVGGISYIAINF
                     VSGLLIGWLMGMPLAETLVICGIMTSSSTAIVAKVLVDLKRTANPETEVIMGMIMFDDL
                     FIAIHMSILSGLVLSGGASLGTVLMTTGSALVFILLFIILGRKAMPFIDRILNGKSSEL
                     FLILVMATLFLVAGFSETLHVAEAIGALLVGLVLADSQHVKRIEHYVINFRDFFGAVFF
                     FSFGLTIDPMTLGGAVGMAIVAVILTIIGNVIAGMLAGRAAGLSSRASINIGFTLASRG
                     EFSIIMANIGKAGGLLPVVQSFAVLYVLLLAILGPLLTKESKRIYNLVDKGIQGSKSLF
                     GKKRVRGKEQSTNSDGV"
     gene            complement(16141..16626)
                     /locus_tag="OF778_RS10165"
     CDS             complement(16141..16626)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725977.1"
                     /locus_tag="OF778_RS10165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="WP_174810904.1"
                     /transl_table=11
                     /translation="MKETELPGIGRKYQLEARNGDQLVIIVHDDGRREMYHFDQQNQEE
                     SISMVTLDDDEARAVAAIVGGLIYKPKALETVNVALDDLMIEWYKVEPHAACVGKSIGE
                     IKVRETTGATIIAILEKDKKIINPGPDVVLSAHTTLVVAGERQHIKLLKPWLLSGSE"
     misc_feature    complement(16666..16680)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 22.76"
                     /tool="antismash"
     gene            complement(16830..17189)
                     /locus_tag="OF778_RS10170"
     CDS             complement(16830..17189)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF778_RS10170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_268624457.1"
                     /transl_table=11
                     /translation="MTRLSVQWEKNSILGVVCVALLLLCSVFSTFMVEEDRVNWNNPSK
                     LTKSFHEQQFIPVSRHTSVMKQLFVKKIVVMFILFFKEFTFSMPPVIRYLHSCFFVIRK
                     RLFLMPIKFTSTFVV"
     gene            complement(17323..17571)
                     /locus_tag="OF778_RS10175"
     CDS             complement(17323..17571)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755677.1"
                     /locus_tag="OF778_RS10175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3951 domain-containing protein"
                     /protein_id="WP_021256622.1"
                     /transl_table=11
                     /translation="MIYFTVMTGMFSLFLMMIVGWVGYKQFVKREPVEVHYTPFDYITA
                     QSSVEFHDEKEDHEVQDGHGDDKDPQSSGAMSTRASK"
     gene            complement(17662..18441)
                     /locus_tag="OF778_RS10180"
     CDS             complement(17662..18441)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794550.1"
                     /locus_tag="OF778_RS10180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_021256623.1"
                     /transl_table=11
                     /translation="MYNENRRNIAIVTGASRSKGIGAAICRELAAKGVDVFFTHLCHYD
                     KETGYEDANGSWPEQFAQELREYGVRVEHMELDYFDANAAVRLLDEVENRLGRPTILIN
                     NATHCIEVDHHHVTAQIIDHHCIVNVRGTMMLSVEFAKRLEAAEVGGRIVNFVSGQDKE
                     PQPGNLAYAASKGAVSAFTTSLAAELASHRITVNAIDPGPTDSGMEAFVQDFLRPKFPT
                     GRIGTPEDAARLVSFLVSDEAEWITGQIIHSDGGFRN"
     gene            complement(18865..19266)
                     /locus_tag="OF778_RS10185"
     CDS             complement(18865..19266)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF778_RS10185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_268624458.1"
                     /transl_table=11
                     /translation="MSAYVIMKANADGAKAVCAPSQSEGELRMKKWMWIVLTVIVLIGG
                     TAAFTIGFVNSTMIEPGKAAVVVQEYEGSVKAIEAERLTIHSGDREEVFRLTGSTDLQG
                     EEFRSIQPGANVRIGVNEGRDIVWLRFVR"
     gene            complement(19316..20107)
                     /locus_tag="OF778_RS10190"
     CDS             complement(19316..20107)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551752.1"
                     /locus_tag="OF778_RS10190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_021256625.1"
                     /transl_table=11
                     /translation="MEAIIKAMLDKLPAWSYQLFAVVIAAGLVFYFIKTLYINKKFTEV
                     IHEVLNREDRMKLLQDKVDNLQVTGGLTEHASSQMLSALRNMKPFADALNGLRGMADPY
                     AVLHESNFLLQRMLDMLAVDMKCAPGGHHRCGIWKYEEPILILQLASVGFPNHYVGQRH
                     LHIDRSVAGRSFRKQVTVQCADVLKDEDWERNQESKSPYRSLICISLGTYGVLTMDGLE
                     PFNESSAVIGELYAAIMTGILAEQQAVLELGEPAQIDSAQG"
     misc_feature    complement(20160..20175)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.04"
                     /tool="antismash"
     misc_feature    20161..20174
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.96"
                     /tool="antismash"
ORIGIN
        1 gcaccatctc cgaacaggat gcaagtattg cgatccgtgt aatccgtaat gcgcgacaag
       61 cattctgcac cgattacaag tgcgtactta tatgtgccca ttgcaataaa gttgcttgcg
      121 ttagcaagtc catatacaaa tccagagcat gctgctgaca aatcgaatgc tgccgccttc
      181 gtcgccccaa gccgcttctg aacaagacaa gctgtggccg ggaatgacat atctggcgtc
      241 acggtagcta ctacaatcaa gtccaattct tctgctgaga tgcctgccgc ttgcagtgcc
      301 ttctccgctg cataaaaagc caagtcagag gttgcctcat gctctgccgc gatccggcgc
      361 tccttaattc ccgttcgtga tacaatccac tcatcgtttg tatcgaccat tttttccatg
      421 tccgcattag tcaacacacg ttcgggtacg tacttacctg tcccaatgat tcctactgac
      481 cgtaagctca tcacgcatca ctcacttccc actgttagtt ccgccgactc ccatagaaat
      541 cgtctcaatc aaatcatttt gcaacgctaa acgtgcttgc cgaaccgcat tatggacggc
      601 tcgcgcatct gaagagccgt gcgccttaat tacaagtcca ttcaaaccta acagcggtgc
      661 tccgccatgt tccttgtaat ccatcgtctt gcgcaagccc ttgagtgctg gcatcaacat
      721 cgctgccgca agcttcgtat accatgtacg cgtgaattcc cgcttcaaca cgccaaataa
      781 ggaacttgcc gttccttcca gcgatttcaa catgacattg ccagcaaatc catcgcacac
      841 gagtacgtca caagtactct ccaatacgtc tctggcttcg acattgccta cgaattgaat
      901 tggcagctcc gcaagcagcg gataaacctg cttcgtcaat tcattgccct tcatcgcctc
      961 tgtacctacg ttcagcagtc cgacgcgcgg cttcgccatg ccatgtacct tctcgcgata
     1021 aatactgccc atcaaggcat actgcgcaag atgctcaggc ttggcatcca tgttcgcacc
     1081 caagtccagc gctagcacgc ctctgccatc catcgtcggg atcattggag ccagtgcagg
     1141 ccgctccaca ccattcatac ggcctacaat gagaagccct gtcgtcatca acgctcccgt
     1201 attgcctgcc gatatcatgc aatccgcttc accttcacga accatacgtc ccgcaacaac
     1261 catcgaggct tccttcttgc gacgaactgc cttaaccggt tcgtcatccg cttcaattgt
     1321 atctggcgca tgaatgatgc gcacattcga tggggaagtg gtcatatacg gagtgagctt
     1381 tgcttcgtct cccacaagca gcagttcaac atccgaccat tccttcgctg ccgccaatgc
     1441 accttctaca ttgctcttcg gcgcatgatc tccacccatc gcatcaatgg cgattctcat
     1501 gcagttctcc tccttcgctg ctaatcaccg ttcctgccga acgataaatg acaaagttcc
     1561 cttggaacac catttcttct cccacataac taaacacttc caccttcgcc ttgcttcgct
     1621 cgcctgaaat tgagcgtaca tacgctttcg cgatacactt ctctcccagc ttcacggaac
     1681 gaagaaagcg aatatccgct gatgacgtta atgcaatctc gtcattaatg accgcaaccg
     1741 caagcgagtt cgcttgcgcg aacagatgat gaccgcgtgc gatatgtgtt cttgagaaga
     1801 cgtgttcatc acggatttca aatatcgata tcccgctgcg atccagctgc aggtcaacaa
     1861 tatcaccaat aacttcatgt agcggcaagg atcttacttg atcgtaggat tgctctgcca
     1921 ttagcttcaa tcgttccctt aattcaggaa tcccgagctc catccggtca agccgaatcg
     1981 tctggatact caccttcaac ttgcgggtta attcctgatc agtcacgaat ggattctctt
     2041 caatgattgc ggccagctgc tgctgacgct gtctcttagg taaacgctcg atgtcccgca
     2101 ccacctttgc tctttgccca atatgctaaa ggcttggctt gccgcataag ctgactgtcc
     2161 aacccttatg tatatcatag atcattttca tcgatttgaa aaaaggtatt tagaacctgg
     2221 tactaagagt agtatatata aaaatgggtc taaaggaaag caccagtttt tatcccccct
     2281 cttttcctcc taatattatc cagggtaaga gaacaaaaaa agtagcaccc gtgagatcac
     2341 gaagtactac ttttcctgca tagattccga ttattgtttg ataatctctc tagctttgta
     2401 tgttccgcac actttgcata catggtgcgc caatttcaac tcaccgcatt gttcgcattt
     2461 taccatgccc ggcacggaca atttgaagtg cgtacggcgc ttgtcacgac gtgttttcga
     2521 cgttcttctt tgaggtactg ccatgtttac acctccttac ccaagttacg tgttatttct
     2581 actgtcagct ttctatttct taaagaaatc ttgtaacccc gccagtctcg ggtcgattcg
     2641 ctcagtcgag catccgcatg gatgttcgtt gcggttcgtg ccgcaagtcg ggcacaaacc
     2701 cttgcacgcc tcgtcgcata gcggcgcata cggcaagttc attaagagcg cctcttccac
     2761 gtaaggcttc aagtcgacaa ggtcgtccgc caccgtaatg acgtcctcct catctccttc
     2821 agcgggaaga tcagtagagt ccttcagctt gaactgctca tcaaacggaa taacgtacgt
     2881 ctcggtaaac gatgttaaac aacgcgagca ttgcagttcc atcttagcgg tcagcttgcc
     2941 atgagctcga gcgaacccat caccttccgc ctttaccgac agttcgacat gaaccggttc
     3001 agaaatggca acatcagagc gctcacgcgc tacctcatcg gcacgaaatg ccgtatcaat
     3061 ctgcgtttcc cctctaccag caactatgtc tcgaaatcga aattccatac tatcactcca
     3121 aacaaacaaa attaattata gcgattaacg tcatgctttg tcaaccaaat tcgtttgaca
     3181 gtttgccaga gccggagcgc gattcccctt cctatggaaa accattcaat catggtatga
     3241 tttgattatc aatacccgtg gattgtctgg gaaagagatc cgtcaataag cattcaatgc
     3301 atctactata gatactattg agataccata ttaccgcatt cagcgcctga aaggaagaga
     3361 cgtgaatgag aacgctcgga atcgttgttg aatacaatcc gatgcataac ggtcatgtat
     3421 atcatctcca gcaatcgaaa aaagtttcaa attgcgacgc agtggtcgct gtcatgagcg
     3481 gacatttcct acagcgtggc gaaccagcct tgaccgataa atggtcgcga acggatatgg
     3541 cacttgcaaa tggtgtcgac cttgtcctcg aattgcctgt ggcctacgct gttcagcctg
     3601 cggaatggtt cgcctacggc gctgttgcca ccctgcaagc gacaggcgta gttgacgctc
     3661 tctgcttcgg cagcgagctt ggtgatgttg aaccgttctt acgtgcagca gatatcctcg
     3721 ttaacgagcc ttcgggcttc acagatcagc tgcaggagca tctgaaggca ggtttaacct
     3781 accctgctgc ttatgccgca gcagcgggac aagcaatgac ggcaagccgc accagccctt
     3841 cttcatccga ggctgcagac tggatggcgc agcccaacaa ttcccttggc ctgcactatg
     3901 taatggcgct ccgccgattg aatagcgcaa tcaaaccgca cacgattgct cgcacacagg
     3961 ccggctacca tgatacggaa gcaccagaag gctcaatcgc gagcgcaacc gccattagac
     4021 gattatggac ggaagaaggc actctcgaga catgcgcaca atatttgccg ccaagcacac
     4081 tgcgtattct tgagcaggag catcgcttcg gcagagcacc aatgagctgg gattgttata
     4141 cgcgtgagct gatatatcga ctacatcagt tgtcccctga atccattgcg cgtcatctag
     4201 aggttacgga agggctcgag tatcgggtga aacaggcact cggccaaatg acagcacaac
     4261 gcgtggaatc cctgcttgag cagctcaaga cgaagcgcta tacccgaacg aagctgcagc
     4321 ggatgctcgc gcatattttg cttcaccatg acaaattcca ttttggatcc gatggcttga
     4381 agaaagggcc ctggtacatt agagttcttg ggttcacgga cacaggacgc gccctgctga
     4441 agcgaatgaa gcaaagcgca gaactgccga ttgtgttaag cgtgaatcgc gataacttca
     4501 atctcggagg ttattgcggc ctggaagctg atatccgagc aacatcgctg tatgcgaacg
     4561 cctttgggcg ttggaatgct cgtgatgctt atcgggacta ttacgagtca cctcggcgac
     4621 atcaatcaaa ctgagggaag aataagcaca aaagatcgag catgggagca tctcgccctc
     4681 acctcgatct tttgtctatc tagccattat ttcatattga gaagatatat cagcctgtat
     4741 tccctcatat ctgcatcacg atagttgccc actccatgat cagacaagct gtgcttcaag
     4801 ctattagagc tcgccgctac ttattccacg agcccttcgc tttactctat acgtttactg
     4861 agcagccttc tgctcggcgc caaacttctg aattgcgttc agtgcatcgc ccactgtacg
     4921 taccgatacc agcttcattt tcgtattcat cgtttcgagc ttcgccttcg cttccgcata
     4981 attgccttct ggagcaagga acagcgccgc cttctcacga tcagcagcca caaccttaaa
     5041 ttgaatgcca ccaatcacgc ctacattccc gccaggatca atcgttcctg ttcctgctat
     5101 ccgatagcct tcagttaagt ctcctggggt taagcggttt atcagctcca aggtaaacat
     5161 aagcccagcg gatggaccgc caatgtcact gtctttgaag gttatggttt tccctatatc
     5221 ctcaggtata gcttccttgc gttgtccgta ccgaagacca aagccaactc gcttctcctc
     5281 tttcttattt ggatccgtca gttccaccaa tgtcgccttt acaagtttct ctttcttgtc
     5341 acgctcaacc ttgacctgca ccgtttctcc agctttgcgg cccttcataa ctgcttgtaa
     5401 tgcttcgaaa tcattaaccg gcttaccatc gacttcgatg atccgatcat tggtctcgaa
     5461 ctcattgtgc gcaaggttcg ggtagttgta aataacatac acgccttcgg atttcaattg
     5521 gtacgggatc ttcaactggt tataggcagc cagtaccgcg cctagctggg aatcactcat
     5581 gttgaacaat tgctcagtca catattcctg ttcgcttctt ccttgtaggg aatctgcctt
     5641 cttcccaaac tcggcatgcg ggtcaaatga tttccataat agcaatgcaa ggttcgcata
     5701 tgtgcggcgc accgtggtaa gcataaatgt cccttcttct gtgtcgtctc cagcttggac
     5761 acttaccatt ggcttcacct gctcggcact gcccggcgta tatatcacat atggtgttgg
     5821 catatacacc atcacataca agaacaagag tgctgcaaag aaccatttcc aactgcctaa
     5881 tcgccaacgc ttcgagcctt ggctgtcacg tactgcttct tcttccatta ttgcattccc
     5941 tcctgctgct tccgctcgcc cctgcttcgt tgtacaaact ggtctaaagc ctgtctttgg
     6001 tacgtacata tgtggaagta ccctacttcc aatactgttg caagaggtga acatcatgtt
     6061 ttcatttcga acgccgctat cccaacaccc gttaacgacg ctgttgatgg gaagtatggc
     6121 agttcttctc gttatttgca ttgtatcgta tccggatcaa gcattccaag catcacttca
     6181 aggcttgaaa atatggtgga acattatatt cccggcattg ctgccgttct tagtattgtc
     6241 tgaaatgctc tacgcttatg gattcgtcca tggacttggt tccttgttgg acccccttat
     6301 gcgtttgttg ttccgtctgc ctggagttgg cggctgggcg tgggcggtcg gctggacggc
     6361 cggctatcca gcaggagcca aggctgttgc ccaactgcgc aatcaacagg ttctatctcg
     6421 tcaggaagct gagcggctgc tctgtctgtc ccatacgagc agtccccttt ttattatagc
     6481 agtcgtaggt gttgggttta tgcagaagcc tgagcttggg ctaatcatcg caattattca
     6541 ctgggcttcc tctatcctaa ctacacttct aatgcggttc attcaccgca tgcgccctga
     6601 gtcgcaacca ttacgcacat ctgcttcact atcgaaatct caccaaaaat tctaccaacg
     6661 gatcattggg cagatggaag gcgcccatcg tcaagatggc cgtcccttcg gcagaatgct
     6721 gggcgagtcc gttacttcag ctgtccaaac cttaatgatg gtcggcggct atatgatgat
     6781 attctccgtt atcattcaag tgctgcgcat cgcaattccg caggagcttg gcaaatatat
     6841 cttgaacgga ttgcttgaag taaatttagc ggcatatacg cttggttcag cagcgttctc
     6901 atctcctgta tttcaagcag cgctgctcag ttccgttatt gcctggagcg gcatcagtgc
     6961 tcttcttcaa gttcacagca ttatgaagac gacggatcta cgcttcggcc attttatact
     7021 atcccgtctg cttcatgcct tatgtgcatt tctgcttact tatatgctat ggtcgccact
     7081 tcattccctg ctccaatcat cagattcagc aatgtcagca ttatgtacgc tagcaaccaa
     7141 cccatccaat gcctatgatg cgcctgtgat cgcggaatca agcagcctac ttccgttatt
     7201 gactttcatt agtcatttgc caatatgggc tcaagcttcc atgctcattc ccgtagcctg
     7261 tctattccta ttcactttat tcatactggc acaaaccgtt ggacggctgt tcacccgagg
     7321 ctaaacaaaa aagtccatat cgaccgttga ttcatactgg cgacatggac ctgctgctta
     7381 cttcgtatct ttgtatttat cacgcagtgc caattctact tcaggtgcca ccaattcgct
     7441 gacgtcacca ttgaatttcg caatttcctt gaccatgctt gagctcagat aggaatattt
     7501 cggattcgtc atcatgaata tcgtctccac tcgtgggtta agcttctgat tcgtggaagc
     7561 catctgcagc tcatattcga agtcggtgac agagcgaatg ccgcgtacga tcacatggcc
     7621 ctgtttctgc tccatataat tgaccagcaa atcacggaaa ctgtcaattt ccacattcgg
     7681 gatatgctga gtaatctgcc caagaagctc cttccgttcc tctacactga acgttggctg
     7741 cttcgacaaa ttgttcaaca ccgctacgat gagcttatca aattgcttag ccgctcgttg
     7801 tataatatca agatgcccaa gtgtaaccgg atcgaagctt cccggataca ccgcaatgcg
     7861 cggctcatgc acttgctctg tatgttccgt catgacaatg gatcctcctt atgccctaca
     7921 gcagattaaa ttcttctgca cgtttcattt cctcatatgg ttagaagctt atgcgtcctg
     7981 ttcaagctca gcttccttct ctgtctgttc cttgacccga tataccgtaa ttccaatttc
     8041 tccgtactct gttttcttcc atacgtagag aggaccgatc tcttcgggat attcatgctt
     8101 cgagtcatgc tcaacgacaa tcgtcgcatc cttcgagatt aacccgtcat tccacatgcc
     8161 ttccagcagt tcgtccgcat ccttcaatcg atatggcgga tcaagaaaaa taagttcaaa
     8221 tgaaatatca cgcttcgtta atgccttaat cgcccgcttg gcatcattgc ggtatacttc
     8281 actctgctcc gcgacccctg ccgcctgaac gttgtgcttc acaacttcga cagccttggg
     8341 atccttgtcc acgaatatcg cttgttccgc accacggctt agtgcctcga tgccaagtcc
     8401 acctgttcct gcgaacagat ccagcacaca gccaccgtca aaatacggcc cgatcatgct
     8461 gaacaaggct tccttgactt tatctgtcgt cggccgcgtc cccatgccgg gtaccggctt
     8521 caacgagcga cctcttgctg ttcctgaaat aactctcaat tcactccacc cattcttatg
     8581 cgcctcagcg cagcttatcc gtataatcat gatggtatgt aacggtaaac ttcttgtatt
     8641 tcgacgttta tcgtaccaca ttttctccta atttgaaaaa ggaaaacgtg tcctcattca
     8701 gacatagctt ctttattaca cgatccctct ataaaaagaa aagctcctta tccggaaata
     8761 gggtttccga taagaagcct tatctatcaa catcaactca tctatgcagc ttctgcagta
     8821 gttccttcac actaacaagc atctgcttgc gcgcttcatc gtcaattgtc gtcacggagg
     8881 caaagaaccg atgtacaagc actttcatgc cgacaaattc aaaaatgccg ctatcagtcg
     8941 tcttctgcat ggcctcatac atgccgctct gctcatattg ctcatgtgtg ttgccatgtg
     9001 tggacaagag cagcgccgac ttccctaaca gcagcttctc tatgccgtca gcaccatatt
     9061 tgtaggcgaa gccatatgaa aacacacggt cgatgtaccc cttcataatg gcaggcatac
     9121 ttgcccacca gattggataa atcatgatga tatgatctgc ccacgtaata tgaccctgct
     9181 cctcctcaat atcagggggc agctcatgct gtccgaacaa ttcgaagtct ttggccgaca
     9241 gcaccggatt gaactccatt tcatacaagt tgcgaaccaa tgcggcatgt ccctgatcct
     9301 ccaattcagc ttgtaccatt tccataatgg catgattgaa gctgttcggg ttgggatgtg
     9361 cgtatataat cagatgcctc aactcgatct cctcctacat atatgaccat cacccctgct
     9421 tatacagctg ggcaagttca ttttggttat aggatgtgag aaagcggcat catttatcga
     9481 gccctacaaa tttttttcga catgtatatt tctgcccaat cgggcaatca ttaaagtatc
     9541 gacatcatcg aatgtcgtag acaaccgcgg agaagactta cgtttcccca taagctcttc
     9601 gtccggtttc tcctctccca tgagggtgtc ctagcaatag ggcacccgtt ttaatttact
     9661 ccaaaccctt atacataaga tttcaaacgt tctttagatg ttgttttatt cagcgagatt
     9721 atcttggtgg ggacttaatg gggacttagc caataaaacg ggatactctg tccaagcaat
     9781 atccaactat gctctacacc gttcactaat ttcactgaag tgatgtatac ggttgctaaa
     9841 gcattatatt gccggatatg atgaactctt caaagtagca ttttaattct catgctttac
     9901 gttcttcaaa gctgttggga gtttcttagc accgaaaacg atctttgctt ttttcgcttc
     9961 cttttcagca cttttctcca attgtttcat tagaaacatt tcaatgttct ttttcatcct
    10021 tgacacctcc tttcggtaat aatgttaagg cttgtacaaa gagaacaatc gatataacac
    10081 tagattgcag gattaagtta ccacttgcta tcaacattgc tatgtactta tacagtatat
    10141 catttttctg gacagtaagt tcctcaaacc aatttggggc tcgaaaataa tagatacaaa
    10201 cggataccac agtcaaaaca acagcagtcg ttttcgatag gagtatcata ggcgttatca
    10261 cacaaaataa tgaggatatg atcatacacg cagttagtga agaaaaatgt gcccctccag
    10321 aaaaccttct aatagcagct aatgccacta atgctagtat agattcatat agatgtccga
    10381 agatagtccc taaagttact gtaatcaaga ttactccata tttatttaat ttcattgcaa
    10441 ttccatattc taaaacctct acatcaacat cgctctcagg gttgttcttc ttcaatttta
    10501 ttgcaatgtt tctcgatatc gcttctaaca ctttcgtaat ctccccgatc cgagtaatag
    10561 taagcaatgc caaagcttgc taatgaaagt atcatgagca atatgtaatt ctgagacagg
    10621 attattaata aagtcaggaa tatcgttact gccgatatca ttcccgaaat aacaacaatt
    10681 cgatgactat actctgaata cttaattctg aatgtatcat gaggcggcac gatgatgaat
    10741 gaaaatcctc ttcccgtcat ccataggaat aaggcaatgg ccgcaccaac aacaatagaa
    10801 cataattgaa tagcgttaac agggaacccc gtataaacaa atattactga tttatcagct
    10861 atccctatgg cggccaaaca gtagtataca atcatttgga tcgatatgta tgccgataat
    10921 cctgatccga taataaatgc tgcataatgc agcctgtatt ccaacacgga atagtaaaac
    10981 attacaaata tgatatattg caagggcaaa tccaaagcag gtaaattaat tacagtcctc
    11041 attaaaaatg aacatattac taatgctacg gataaaacaa gtattttata cttgtacctt
    11101 aataaaggct gcttatatat ctttaggatc aaagcattaa caactgcagc atcgagggta
    11161 ctaaataata tgtaagttaa agactgactc actttgcacc acccttttag aataatatat
    11221 gactattata ctatttgatg tggtaaaagg aattaaaaag ccctggaaaa atccgaagtt
    11281 ttacttgctt tatagcacat gtaagataac cctaatactc acccattggt ttcatcttca
    11341 tatagggtgc atgaataact ccatccctaa tgcttcgcac cactagtgga aaatacagat
    11401 gagctacata accaatagtg cgttttcctt atcccatagg tgtacaccac tgttgcatac
    11461 tgtatgtctc atatcttaac tgatctccac atgataaatt ttaacaaact gcaaagttag
    11521 cggagtcccc tccactggca cctcatacag cgttagtagt cccgtttcta ccttgtaaac
    11581 attaagcggc tgaagcatgc cattaagaaa taggttcgta taagatactg tcaacggatt
    11641 cagaatgtct gagcttccat actgaggctg cgcctcggca ttcgtatatt catatttcaa
    11701 cccatcgcaa attgcaacat attggaaagt atcaacctta agtattttgt ccttctctgg
    11761 cggaggcggc tgagggggag gagatgggaa aatagaagca cagctctggg gcggcataat
    11821 ggtgcttgat gttgcactgc ttgccgcgta aggatgttca attttaataa tccatggttt
    11881 ggctgttagg cgaattacac cattaccgtc attcatcact ctatgtcacc cgtcctcttt
    11941 atctgttgtc gttcggattc ggtacacttt ttattatgtt attcaagcac atcccttctg
    12001 ctcatggcca tctgtacatc ctcctctcct aaattttgca acacaaaatg gctcccccta
    12061 ggcaaaaatc aatctccata gagggaacct gcacgcgact cacatcattc tcaagatagt
    12121 atacttacgt aataacaaat tcaatcaata ttggagttgc gccatcaagc gcgtctcctc
    12181 ctggaatcgt cacattcgtt gtcgtgatcg tagacgtatc tcctgtctga agcacaccat
    12241 taatatacag attgtagtac gcaaacgatg aaggaaacgc tgtagcagct gcacctgtat
    12301 cattcgtgaa gctagtggct gctaccgcaa acgcagcacc agttccagta cctgcagata
    12361 acgtagccgc aaatcttctg ctcgcgagaa aaggtttaat aattgccaat tgcttgcacc
    12421 tcccattaca aatcatcatt cagcttatgt ggaacaacaa catagctcaa gagactgctc
    12481 agagcaattc tctctttata ttaaatgcga gaaatctatg aatgaaacgg tatttaacag
    12541 cgtatacctc cctatttgta acctgtaaaa cctcacgact gacactggat cagacatttt
    12601 tgtatgcaaa gagagcttcc tagtatttca ctagcgggaa gctctgtacc accttttctc
    12661 aatctatctg acagcataac ctacagctcg tccatactgc agtaaacgct tgcttcttgt
    12721 aagcaaatgc tatttctcat ccttttcctg ttcatcgtct ttcttagatt ctttcttcgg
    12781 gataccctct tcatttccgc ccttatcctt caatacaaag atcgcctttt tgattacatt
    12841 tgggactggc acaccgagtc ggcctccatt ctcaacaatc gacaacactt cattagccat
    12901 ataaaaatag gttgtcgcat ctcgaatcat ctctttgctg tcaaacgcga tatcgatctg
    12961 atgagcgagt gcaataatga agaaaatgaa tactttgcgc gctatcccga taagaccgac
    13021 ccggctcaac aatttccctt caacagcgga agctaacatc cctgtcacat aatctaatat
    13081 taccatcgtt aacagaacct gaactaacgc aggccatcct ccaaatacaa acgaaaacgt
    13141 ccctccaaca gctgcagcac ccgttttgag tagaagattg aatcggtcca tgcttgccct
    13201 cctctgcagt cgtatacact ttggcctgta tctagcactt tcaatgcctc tgctagagta
    13261 ttgacgaagc actcgcagac cctgactata tgttatgcag catgctcgtt gcccgacaaa
    13321 ggcaagcctc ctaactcgct ttcattgcac aacaatggtc aggatgattt ttgatttact
    13381 agaatgaaag atttgcactc agtcaaacaa tggcctgacc tccgcttccc cccgcaattg
    13441 atcaacagca aaatacggta ccccacagag taccgtttcg tttccctatg cccctattcc
    13501 ttattcaata ttccggctcg cttcaattgt ccctttacct tggtctatcc gttcatggct
    13561 cacttgaatg gatgatccaa tgtccatcga aatccttgca ggaatgagtt cgaaggtaga
    13621 gacaactgcc cgatgatcag acgaccatgg cgtaatcgca atatcagtat agggacctct
    13681 ctcgcctaca atacggctgt caatcgtagc agacggccct cccgcataga caaaatcaat
    13741 tcgatcatgg acttcatccg gttcaatatg tccattccct cctggcgtcc aagtcacggc
    13801 cggcttcatg agcggatccg agtgcacttc ccgataggaa tctcgaaact gtagctgctc
    13861 caatcgtatg gataccggcc attcgacagc tacgccaaag tgcatgctct tcgcttcatc
    13921 tgtccaatcc agatgggatg gagcattgaa atcgcctgtg agaaacacgg gaacgccttg
    13981 acttgccaat tgtccgagca gatggaaccg actgttcatc tcctgcatat gaattcggtt
    14041 ctcgttcgcc accgcagctg cagcatccag cccatcccgt atgtcatagg gtccatacgg
    14101 ctcgtggcac aggtgaacat tgctcatggc agccacctcg tccggcgtaa cctctatcca
    14161 tatgaactcc ttatgcgccg attgatgaag tggatatcgg ctcaaaatac tgtgcccctc
    14221 atcatagtaa tagttcagct tgcgagcgag cgcaggaagc tgtccgtctg cttcttgtat
    14281 gccgatcaca tccgcttcta cggttcgaat gacttccgta acctgaacaa tcgaagtact
    14341 gcctctccag atattgaatg tcattacctt taggttacgt ccgctctcca tttcttgaac
    14401 aaacgtatcc cgcttgttcc tcatcacata actccctccc aacccagtaa cccagttgtc
    14461 ctctaagaga aaaacaaaag ccgctgaatg ctcagcggct caaattcgta accatcatag
    14521 atggaatgtt ccattacgca tagggttctg ctagacagta ctcgtcctcc atatggcgaa
    14581 tccattcttc cagctcatcc agctgttcga aggttcgtac cgcaatagga gcacggttag
    14641 gtacgtctcg ctgcaagatg acagtatcgc ccgccatcat ccgttccctt gcatgtctat
    14701 agattgtatc cacccgtttc caatccttgt gttcataagc gctcccaatc tgcgtgagat
    14761 cgatagtgat attcatacgc tatccctccc ttgaatgggt atgccttaca atatataata
    14821 taacctattt ttctcaaaac gtacccgatt tttaaagatg agccatccaa ctatacacca
    14881 tctgagttcg tgctctgttc cttgccacga actcgcttct tcccgaatag cgatttactc
    14941 ccttgaatcc ccttatcgac caggttgtat atgcgcttgg actccttcgt taagagtgga
    15001 cctaagatcg caagcaacaa tacatatagc acggcaaaag attgaacaac cggtagcagc
    15061 cctccagctt tgccgatatt cgccataata atggagaact ctcctcttga ggccaatgtg
    15121 aaaccaatgt taatggaagc acggctcgat aagcctgctg cgcgacctgc cagcatccct
    15181 gcgatgacat tcccgattat tgtcaagatc acggcgacaa tcgccatacc aactgctccc
    15241 cctaatgtca tcgggtcaat cgttaagccg aagctgaaga agaacacagc cccaaagaaa
    15301 tcacggaagt tgatcacata atgttcaatc cgcttcacat gctgggaatc agcaagtaca
    15361 agtcctacta atagagctcc aatcgcctct gccacatgca gtgtttccga aaatccagct
    15421 acgaggaaca atgttgccat caccaaaata aggaacagtt ctgatgattt cccgttcaag
    15481 attcgatcaa tgaacggcat cgccttgcgg cccaaaataa tgaataacaa aatgaataca
    15541 agtgcagatc ccgttgtcat cagtaccgtt ccgagggatg cgcccccgct aagtacaaga
    15601 ccggacaaaa tggacatatg aatggcgatg aataaatcgt caaacataat catgcccata
    15661 atcacttcag tttccggatt cgccgtccgt tttaaatcga ccaatacttt cgctactatc
    15721 gcggttgatg aacttgtcat aatcccgcaa atgaccagtg tctccgcaag tggcattccc
    15781 atcagccaac ctatcagcaa gccggagacg aagttaatcg caatatagga aattcctcca
    15841 accacgatgg atttccctga tttgagcaag cgtcctacgg agaattccag ccccaagtag
    15901 aacaacagga acagtacccc aatacgaccc ataaaatcaa taaagactga actctcaatg
    15961 aaacggaagt caaatatcca aaaatgcggc gcgtgcggcc caaccgccat cccgattaga
    16021 atatagaaag gaatgacaga gaagcgaagc ttcgttgcaa taacgcccac tagtccaatt
    16081 aaagcaatag ctaatccaac ctcgaaaata atcgtatcca tagttcaccc cgttttctgc
    16141 ttattcgctt ccacttaaca gccaaggctt aagcagctta atatgttgtc tttcccctgc
    16201 aacgacaagc gttgtatgag cagataacac gacatcaggt cctggattga ttattttctt
    16261 atccttttca agaatcgcta taatcgttgc ccctgtcgtt tcgcgtactt taatttctcc
    16321 gatgctcttg ccaacgcagg ctgcatgagg ctccaccttg taccattcaa tcatgaggtc
    16381 atccaaggca acattgaccg tctcgagtgc cttcggctta tagataagcc caccaacgat
    16441 agcagcaact gcgcgcgcct catcatcatc caatgtcacc atcgaaatac tctcttcttg
    16501 attctgctga tcaaaatgat acatttctcg tcgtccatca tcatgcacta tgatgaccaa
    16561 ttgatctcca ttgcgagctt ccaactgata tttacgaccg attcctggca attctgtttc
    16621 tttcatattc aaatgaaatc cctccaaccg tccataggtt tatttttctc acattgtgaa
    16681 ataaggaact ttatatacat gtcaaataca tctttcttgc agcacaaaat aatccatgta
    16741 aataacgatt tttaatgcca tagagaacat cttgcactgc tattccttat tcaatttcaa
    16801 gcacagtctt gtgaatgaat gtaagagatt tacactacga acgtagaggt gaatttgata
    16861 ggcatgagaa ataaacgctt ccgtataacg aagaaacagc tgtggagata cctaataaca
    16921 gggggcatac taaaggtgaa ttccttgaag aagagaataa acatcactac aatcttcttc
    16981 acaaatagct gcttcatgac agaggtatgc cgtgaaaccg gtataaactg ctgctcatga
    17041 aaagacttcg tcagcttgga aggattgttc cagttgacgc gatcttcctc gaccataaac
    17101 gtggaaaata ccgaacacaa aagcaggagg gcaacacaca caacaccaag tatgctgttt
    17161 ttctcccact gcacactcaa tcttgtcata tcgcaccccc tgtcgttagt taatcgtaca
    17221 tttttttatt agaaataaag attatcatgc agatctgtat caagcaagaa agatcgaata
    17281 tccattctct ggatgaacgc gattccgctc aactgcaatg gttcacttcg acgctcgagt
    17341 cgacattgcg ccagatgatt gtggatcctt atcatcacca tgaccgtcct gtacttcatg
    17401 atcttccttc tcatcatgaa attccacgct cgattgcgct gtaatataat cgaagggcgt
    17461 ataatgaacc tcgaccggct cccgcttcac gaattgcttg taaccaaccc atcctacgat
    17521 catcatcaga aataaactaa acatgccggt cataactgtg aaatagatca ttgcatcctc
    17581 ctcagtcgca gaaatgagcg ttactagata ctacgcagta caagtggaaa cgtttcattt
    17641 ttttcataat cattcattcg tctaattacg gaagccccca tctgaatgga taatctgccc
    17701 cgtaatccat tctgcttcgt cactcaccag gaatgacact aagcgcgctg catcctctgg
    17761 tgtaccaatg cgccccgttg ggaacttcgg tcttaagaag tcctgcacga atgcttccat
    17821 tccggaatcg gtcggaccag gatcgatcgc attcaccgta attcgatgag aggccagttc
    17881 agcagccaac gaagtagtga aggcggatac tgcccccttt gaggctgcat aagccaagtt
    17941 gccaggctgc ggttctttgt cctgcccgga cacgaagttc acgattctgc cgcctacttc
    18001 cgcagcctca agccgcttcg cgaattccac ggataacatc atcgttcctc ttacgttcac
    18061 gatacaatga tgatcgataa tctgtgccgt cacatggtga tggtcgacct ctatacaatg
    18121 tgtagcattg ttaatgagga tcgtcggtct gcctaggcga ttctcgactt catccagcag
    18181 ccgaacagca gcattggcat cgaaatagtc gagctccata tgctccaccc gaaccccgta
    18241 ctccctaagc tcctgtgcaa attgctctgg ccagctgcca ttcgcgtcct catagccggt
    18301 ctccttatcg tagtggcaca agtgtgtaaa gaacacatcg acgcctttcg ctgcaagctc
    18361 tctgcaaata gctgcaccaa ttcctttgga gcggcttgct ccagttacaa ttgcaatatt
    18421 acgtctattc tcgttgtaca tatttatcgt ctccttatgg gtaaggaacg gcaatcgcaa
    18481 gcgtatcgga gatgcccatg taccaaaaaa agcagacacc catggcgtct gcttggtcag
    18541 ttcatagcac ttaaccttgc gttccttgta atgggtataa atagacacac ctatcccgtc
    18601 atcgaatctc gttcggaatg gtacgatcta ttcagttacg gctccattac aaagtccaca
    18661 tattaaatat gaatcccctc tcatacatct cctatgccct attgtatgcc tacattcgta
    18721 atatatcaag gttttctcgc catcatccgc cgcgggctgc acgtactgtg cttgggatga
    18781 gcgcactgtc aaaaaaagaa gcccggcaga atgccaagct cctcttctca tgctttttgt
    18841 cattgcatca tgcgccattt tcaactagcg aacaaatcgc agccatacaa tatctcttcc
    18901 ttcattgacc ccaatgcgca cgtttgctcc aggctgaata ctccggaact cctccccctg
    18961 aagatcagtt gatccagtca agcggaatac ttcctcacga tcccccgaat gaatcgtaag
    19021 ccgctctgct tctattgcct tgacactgcc ttcatattct tgaacgacta cagccgcctt
    19081 gcctggctcg atcatcgtgg aattcacgaa gccaatcgta aatgcagcgg ttccaccaat
    19141 aagtacgatt acagttagga caatccacat ccattttttc atacgcaatt ccccttctga
    19201 ctgactcggt gcacataccg cctttgctcc atcagcgttt gccttcatta taacataggc
    19261 actcatgtac ctcaccttat caatgactac ggtgcttata ctaagttcct gatgtttatc
    19321 cttgggcaga gtcaatctgg gcaggttcgc caagctctaa taccgcctgc tgctcggcaa
    19381 gaattcctgt cataatcgct gcatacaatt caccgatgac cgcactggat tcattgaacg
    19441 gctccaagcc atccatcgtc aatacaccgt acgttccaag tgaaatgcaa atcaatgagc
    19501 gatagggact cttcgattct tgattgcgtt cccaatcctc gtctttcagc acatctgcac
    19561 attggacagt aacctgctta cgaaaggaac gtcccgctac agagcgatcg atatgaagat
    19621 ggcgctgtcc aacataatgg ttcgggaagc caaccgaagc aagctgtaga attagtatgg
    19681 gctcctcata cttccaaatg ccacagcgat gatgaccgcc tggagcgcac ttcatatcta
    19741 cggcgagcat atccaacatc cgctgcagca ggaagttcga ttcgtgaaga acagcatacg
    19801 ggtctgccat tccccgcaac ccattaagcg catcagcgaa cggcttcata ttgcgcagcg
    19861 cggacagcat ttgagaagat gcgtgctccg tgaggcctcc tgtcacttgc aaattgtcca
    19921 ctttatcctg cagcagcttc attcgatcct ctcgattgag cacctcatga ataacttcgg
    19981 taaacttctt gttaatatag agtgtcttaa tgaaataaaa caccaatcct gccgcaatca
    20041 caacagcaaa gagctgatat gaccatgccg gcagcttgtc aagcatcgct ttaatgatcg
    20101 cttccatatc gtccctcctc tcttacctgt ccttggatca agaatatgac ggagaatgag
    20161 cgaacatgca ttctgccttt gatttttttc gaatataaca agaaagaacc gttcagtcct
    20221 ccctgatcag gaagcaaccg aacggttctc tgtatcccaa actcgtgctt gattatccgt
    20281 gttgatgccg aactgcaggt cgtaccggga tggaacgact gcgtgtcgtg ccaatctgta
    20341 ccgtccacgt aattaccttg cctgctatgc tgacagctag tatcgtatac agcgtgtcga
    20401 caagcggcag gaagaataag gacagcagca ataccgctat atcaaaaata ataaatactg
    20461 tacctatgct taagttcatc ttacggctca taaataatgc tagaatgtca tcaccgcctg
    20521 aagcgcctcc a
//
