LOCUS       NZ_CP086141            20585 bp    DNA     circular CON 20-JAN-2024
DEFINITION  Clostridium estertheticum strain CF016 plasmid pCF016-270, complete
            sequence.
ACCESSION   NZ_CP086141
VERSION     NZ_CP086141.1
KEYWORDS    RefSeq.
SOURCE      Clostridium estertheticum
  ORGANISM  Clostridium estertheticum
            Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 269589)
  AUTHORS   Wambui,J., Stevens,M.J.A. and Stephan,R.
  TITLE     Clostridia strains as spoilage organisms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 269589)
  AUTHORS   Wambui,J., Stephan,R. and Stevens,M.J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2021
            Assembly Method                   :: unicycler v. 1
            Assembly Name                     :: CF016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Nanopore/Illumina miniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 05:10:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,249
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 5,019
            CDSs (with protein)               :: 5,019
            Genes (RNA)                       :: 135
            rRNAs                             :: 15, 14, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 13 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 22 of 95
            Pseudo Genes (incomplete)         :: 78 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:27:22
            NOTE :: This is a single region extracted from a cross-origin
            section of a larger, circular record.
            Orig. start                       :: 264921
            Orig. end                         :: 15917
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086141.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     misc_feature    complement(127..141)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.58"
                     /tool="antismash"
     misc_feature    237..251
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 21.74"
                     /tool="antismash"
     gene            360..1154
                     /locus_tag="LL065_RS25265"
                     /old_locus_tag="LL065_25250"
     CDS             360..1154
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392603.1"
                     /locus_tag="LL065_RS25265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_25250"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_226139130.1"
                     /transl_table=11
                     /translation="MKKIIKVGMSILLFTAVLTAFGANNKANAAAIWSSSQKYGSWPNG
                     GYTLNNDVWSQDTVGPQTIWANSYSNWGTWSAQPNTGGIKSYPHVEKAINKKLSTVKTA
                     TSSFNVTVPTSGTSMETAYDIWLSDPNAYDPTHPSKNKHEIMLWMNQYGAVNPISYKWD
                     SAGPIPVYKNVSIGGHTWNVYIGNNGLNNTGNMVYSFIRTNGNISSGSLDIKAIANWIK
                     NTPKWYGDILFDNIQFGYEITSSYNGGKGYNFNTNTFSVIFN"
     gene            1327..2370
                     /locus_tag="LL065_RS25270"
                     /old_locus_tag="LL065_25255"
     CDS             1327..2370
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062262.1"
                     /locus_tag="LL065_RS25270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_25255"
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="WP_226139128.1"
                     /transl_table=11
                     /translation="MIKKLSNGELDLNVLEIKDIIDIKLLQTFQDNFATALTMSSVTVD
                     KDGIAVTKPSSYTRACLSFTQSTKIGHERCALSHKKCGEEATKTGKPYIHTCHAGLLDF
                     AAPISVGDYHIGSILGGQVFTAAPSEAEFRKTANEIGVNEDDYVKAINEIHITHEKNVI
                     AAAEVLSIVTNSLSQAGYEKLKLSIMSQTLADNFSQISATMEELSASSITVTNNQHNLS
                     EEIINVKNISIEINSILDSIKSIADQTKMLGLNAAIEAARAGEAGRGFGVVATEIRALS
                     QNSKETAVKILSLTTKIQNSVDKTIETSHATLETTEQQSAAIQEVTSTLMEVTTLADEL
                     NAMANDN"
     gene            complement(2522..3631)
                     /locus_tag="LL065_RS25275"
                     /old_locus_tag="LL065_25260"
     CDS             complement(2522..3631)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391595.1"
                     /locus_tag="LL065_RS25275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_25260"
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="WP_326493103.1"
                     /transl_table=11
                     /translation="MKKLLKHLRMVILVIAIAISSFGVLQAKAATTNQTLLNTYGGNFG
                     RVGSCVTLAQLQDPATLSVIKQRYNSITLENEMKPDALLGLSPTLLTVSQAKALGYYIP
                     SNYTETTVPKIDFTAVDKALQICYKNGLGVREHTLIWHSQTPSCLFNTRYSSSGSYVSS
                     TVMDARMEFYIKTVMNHVHSSNYGSVVYAWDVVNEYLHSPSTSGWVKIYGNVTTSPQFV
                     KNAFRYAYETTSYFGLTNKVSLFYNDYNEYMESSNIIKMINYINSGTRYCNGIGCQSHL
                     STSFPSVDYYKSALYAFMNAGLEIQITELDAGATTETEQAAYSYNIMKAICEVKKAGAN
                     ITGITWWGLSDNVSWRTDNPLLFSTLYVK"
     misc_feature    3925..3940
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.53"
                     /tool="antismash"
     misc_feature    complement(4487..4501)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 19.04"
                     /tool="antismash"
     misc_feature    4601..4615
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.02"
                     /tool="antismash"
     region          1..20585
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20585
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20585
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:10585](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     gene            complement(join(4603..4668,4669..4854))
                     /locus_tag="LL065_RS23830"
                     /old_locus_tag="LL065_23810"
     CDS             complement(join(4603..4668,4669..4854))
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS23830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_23810"
                     /product="hypothetical protein"
                     /protein_id="WP_226139126.1"
                     /transl_table=11
                     /translation="MSTRKSNSKPKTKTNELILLEAKENHKFIISFIDNRHFLPFLNIV
                     LSTVFIGIIASICIYKGQLANTAILVSDMLMKLFVGRK"
     proto_core      complement(10001..10585)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            complement(5526..5975)
                     /locus_tag="LL065_RS23835"
                     /old_locus_tag="LL065_23815"
     CDS             complement(5526..5975)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /locus_tag="LL065_RS23835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23815"
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="WP_226139202.1"
                     /transl_table=11
                     /translation="MNTSGSTITDLEIYGAADNGIYMEGSSNRLTNLKVHGNHDAGVQL
                     SNGAASNTLTNVYSYSNADAKGENTDGFAIKLHSGAENKLIDCTAEGNSDDGYDLYAAH
                     GAVTFTRCKAINNGNCGGIKGDGNGFKVGGVDNKTPGVCRSIYAL"
     gene            6191..7519
                     /locus_tag="LL065_RS23840"
                     /old_locus_tag="LL065_23820"
     CDS             6191..7519
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582492.1"
                     /locus_tag="LL065_RS23840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23820"
                     /product="IS3 family transposase"
                     /protein_id="WP_226139124.1"
                     /transl_table=11
                     /translation="MSKILFTKKDIIKLEKNKNVKNVSVCGITYDFKFKMLFINEYIAG
                     KLPRQIFAENGFDIDVIGLKRIEQSAYRWNSAYKKDGIIGLEDTRKTASGRPRSTELTK
                     EEIIVRQNARINLLEAQVELLKKLDAKERQVVQKNKKIKSSDAFKTIKEVIEKYSFVNV
                     VGYLCELSGVSRSGYYNYIASKDIRVEREQRDLEDRDLILKAFNRRGYKKGSRSIKMIL
                     VNEFNITYSLKRIRRIMNKYEIICPHRKANPYRRIAKATKEHSVVKNILNREFKQATPE
                     KVLLTDITYLPYGNSKMAYLSTIKDSSTNEILAYQTSDRITLDIALDTINKLVKNRRVT
                     LTKDAFIHSDQGSHYTSPTFQKLLKKHKLSQSMSRRGNCWDNAPQESFFGHMKDEIDLK
                     SCTTLEHVKKMIDRYIKYYNNYRYQWNLKKMTPVQYRNHLLAS"
     gene            complement(7667..8401)
                     /locus_tag="LL065_RS23845"
                     /old_locus_tag="LL065_23825"
     CDS             complement(7667..8401)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390655.1"
                     /locus_tag="LL065_RS23845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23825"
                     /product="hypothetical protein"
                     /protein_id="WP_226139122.1"
                     /transl_table=11
                     /translation="MKKIKMKVITSCLSLGLVAGTLFTPVSAFAGTMGDPTTQLDGRLS
                     TFHQGAANDCGAVSGIQALANSKFGKKFLNKVISVNSDGSYTLNFGSGKQTVSETDVAN
                     AYISGDLDARVIEAGMQKAMNVYNGCFACDVFAKMTGFGQNIVSGTTAKANMMNTMALK
                     CKSGDGITAACDFSIADPSKGIIGDGGHSYSIKSVTKDTVVVINPWDTSKFINMSRSQF
                     ESSIRYMTYVDETTNKIVVFWN"
     gene            complement(8710..8883)
                     /locus_tag="LL065_RS23850"
                     /old_locus_tag="LL065_23830"
     CDS             complement(8710..8883)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /locus_tag="LL065_RS23850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23830"
                     /product="HTH domain-containing protein"
                     /protein_id="WP_226139120.1"
                     /transl_table=11
                     /translation="MLKTMELSWVRPTRSLHKEFNKHYSKWENKEATGVEFAKLFNISR
                     ATLYRHIKEYQM"
     gene            complement(9515..9904)
                     /locus_tag="LL065_RS23855"
                     /old_locus_tag="LL065_23835"
     CDS             complement(9515..9904)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="LL065_RS23855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23835"
                     /product="response regulator"
                     /protein_id="WP_220705879.1"
                     /transl_table=11
                     /translation="MEGSKINIIIADDNKDFSNILNDYISMQKDIVVTGIAANGVEALD
                     LIQEKKPDVVILDMNMPIMDGLGVLERLTTMNFDIKPHILVLSAVAKKTTTQKALALGA
                     DYYILKPFSIETLVKKIRQIMNVAI"
     gene            complement(10001..10585)
                     /locus_tag="LL065_RS23860"
                     /old_locus_tag="LL065_23840"
     CDS             complement(10001..10585)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103700.1"
                     /locus_tag="LL065_RS23860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23840"
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_226139118.1"
                     /sec_met_domain="AgrB (E-value: 4.4e-42, bitscore: 137.7,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKLVSYLVKKISETNSQFTELELKKMNYGFICLFDEITKIIPYY
                     IIFYLFSLQQYYIVIFVFFCPIRLFSGGFHAKTYWGCFFISFVTFCVMIIIGKYITINI
                     LTSIVVLIISILLVYIFSPVDNINKRIKSKERRNKLKYLSVVITSLLSGISYIIPDEFF
                     TTAVLAIFIAVMMMMMGCINNNKDKNCSSDN"
     gene            complement(10601..11911)
                     /locus_tag="LL065_RS23865"
                     /old_locus_tag="LL065_23845"
     CDS             complement(10601..11911)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103701.1"
                     /locus_tag="LL065_RS23865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23845"
                     /product="ATP-binding protein"
                     /protein_id="WP_264174826.1"
                     /transl_table=11
                     /translation="MIIEIILDIFEALLLVGTFSLLTNQKKYIVENKIRSGLFCMSYII
                     TAYLSTNYFNDNLYHTLVTIIVSIVLLTFITKTNVYSSIIIFSLFFTMIFVTETLIQFI
                     EMFILKVELNEILTIYKYALIFRIGSKLLQILVLIIISKYNIFLNKRELFNSEKSLFSK
                     FFIELGVFSLFLFVLLFSVLDIKNNKIYGILIFNSFFIFLLIAFMHLKKNVELINISNK
                     YKVQQYQISNMEEIINIIRQEKHDYANHINVIQALCCLNKPNTVERIKEYVSKLSDIIH
                     LSFRYLDTGNDYLDGLLSIKNNYATQNNIDFKVLINESFSLLKIREDELISIISNIIDN
                     AFEALMLKALDDNKEISITTLLDDNKFCIEISNNGSGIPKEIINKIFEKGYSTKTKGKS
                     DHGYGLYITKQLVEHNNGSISVERTSLKTKFLIEFLM"
     gene            complement(11988..12122)
                     /locus_tag="LL065_RS23870"
                     /old_locus_tag="LL065_23850"
     CDS             complement(11988..12122)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS23870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_23850"
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_226139116.1"
                     /transl_table=11
                     /translation="MLKKLICKTITTMGCLGLLLGNVAIMPASLLHAYEPNCPDEFLR"
     gene            12674..14092
                     /locus_tag="LL065_RS23875"
                     /old_locus_tag="LL065_23855"
     CDS             12674..14092
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624792.1"
                     /locus_tag="LL065_RS23875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23855"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_226139114.1"
                     /sec_met_domain="Aldedh (E-value: 1.1e-33, bitscore: 110.6,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIIKRITFLILIKFYNKRRKKIMNKDNLYLGVFEDVEIAISKAI
                     HAQKVLTTDYKEEDRERLINSIRKATLKNKETLAKMIIEETNMGRYEDKILKHELAAKY
                     TPGTEDLTTTAWSGDQGLTVVKMSPYGVIGAITPSTNPTETVICNSIGMIAAGNSVVFN
                     GHPGAKKCVAFAVDMINRAILSAGGPANLVTTVKNPTMESLDVIMKHPSIKLLCGTGGP
                     GLIKTLLNSGKKAIGAGSGNPPVIVDDTADIKKAAKSIIDGCSFDNNLPCIAEKEVFVF
                     ENVANDLINNMLQNNSVLINKEQVSKLLDLILIKKDEKSYNHNKKPEYNINKKWVGKDA
                     KLFLDQIDVEAPTNVKCIICEVDINHPFVMTELMMPILSIVRVKDIDEAIECAKIAEQH
                     KRHSAYIYSKNIDNLNRFETEIDTTIFVKNAKSFAGVGSGAEGFTTFTIAGPTGEGITS
                     ARDFTRQRRCVLVS"
     gene            14165..14818
                     /locus_tag="LL065_RS23880"
                     /old_locus_tag="LL065_23860"
     CDS             14165..14818
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424584.1"
                     /locus_tag="LL065_RS23880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23860"
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="WP_226139113.1"
                     /transl_table=11
                     /translation="MNKIFEMEELKSIFKDGMTIMIGGFLDCGTPDTIIDMLVDLNIKD
                     LTIIANDTGFPDKGIGKLVVNGQVKKVIASHIGTNPETGRRMNTGEMEVELSPQGTLIE
                     RIRAAGSGLGGVLTPTGLGTIVENGKRVITISNTNYLLELPLKADVSLVKGSIVDEFGN
                     TYYKATTKNFNPYIAMAAKTVIMEAESLVKCEQLRTEDIMTPGVLVNYIVKEAV"
     gene            14818..15483
                     /locus_tag="LL065_RS23885"
                     /old_locus_tag="LL065_23865"
     CDS             14818..15483
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395722.1"
                     /locus_tag="LL065_RS23885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23865"
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="WP_226139112.1"
                     /transl_table=11
                     /translation="MITDSKLAKEIIAKRVAKELKDGQLVNLGIGLPTLVGNYVPKGMH
                     ITFQSENGMVGMGPKAKPGQEDTNLINAGGQCVTVLPEGSFFDSSMSFALIRGGHVDVT
                     VLGALEVDEKGNLANWIVPGKMVPGMGGAMDLVMGANVVIIAMRHTGKGKPKILKKCTL
                     PLTAKSQVNLIVTELCVIEVTANGLLLKEIHKDTTIDEIKSVTEATLIIPDNIKIMDV"
     gene            15529..16275
                     /locus_tag="LL065_RS23890"
                     /old_locus_tag="LL065_23870"
     CDS             15529..16275
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615002.1"
                     /locus_tag="LL065_RS23890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23870"
                     /product="acetoacetate decarboxylase"
                     /protein_id="WP_226139111.1"
                     /transl_table=11
                     /translation="MLESEISKQITTPLTAPAFPKGPYKFHNREYLNILYRTDLASLRK
                     MVPEPLELDEDEPLVRFEMMAMPDVTGLGSYTECGQVIPVKYNGIKGDYLHMMYLDNHP
                     AIAVGREISAYPKKLGTPKLFVDSDTLVGTLNYGSLRVATATMGYKHEPLDIKEAYNQI
                     SRPSFMLKILQGYDGKPRICEIICTQNKDITIHGAWTGCARLQLFSHALAPLADLQVLE
                     VVSASHILTDLTLASPHVVYDYLAKN"
     gene            complement(16409..16900)
                     /locus_tag="LL065_RS23895"
                     /old_locus_tag="LL065_23875"
     CDS             complement(16409..16900)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS23895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_23875"
                     /product="hypothetical protein"
                     /protein_id="WP_268064756.1"
                     /transl_table=11
                     /translation="MSYLILGFSLLLIFLMISSKNIFYKYSDKINLKIKKRLDTMLKFT
                     KIAPIIVLFIILTLTLTYFKTKYAIRLSHAWLVISFWMCTIIFYYIIAEIVIIKKVDII
                     IPTIGLIISMCNAIYLTPLLHYENIFQNINIMIPNLFGLIMLIVAYYITYLFLKKGIKK
                     "
     gene            complement(16971..17438)
                     /locus_tag="LL065_RS23900"
                     /old_locus_tag="LL065_23880"
     CDS             complement(16971..17438)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS23900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_23880"
                     /product="hypothetical protein"
                     /protein_id="WP_216194683.1"
                     /transl_table=11
                     /translation="MSENKEYTFKREPSEPFNNLIYTVCIFPILFHIRDFTANSIYERL
                     YGLFIAGTFIVLILNQLYIIITKKYKLSDLIIDNNHIAIKKWFLMRYYTIDLQQIKNIT
                     ELLLTSDNLIEITLMNNKKIRLSLKGYSNNDKKLISNQFKVINSNLKIQLN"
     gene            complement(17703..18560)
                     /locus_tag="LL065_RS23905"
                     /old_locus_tag="LL065_23885"
     CDS             complement(17703..18560)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420431.1"
                     /locus_tag="LL065_RS23905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23885"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_226139110.1"
                     /transl_table=11
                     /translation="MGWIEEIGEAINYIEENITEEIAIKNVAKKVLMSPFYFQKGFAML
                     CGFTVGEYIRQRRLTIAGSELVSTDEKIIDIALKYGYASPDSFTKAFTRFHGVTPTAVR
                     KDGAMIKSFAPLKIKFSLEGGYIMDYKIVKKDSFTVIGVSKVFKYDNATTEIPQFWTEH
                     YQTGKGKFVCGMYGVCIDDSMGSDEFEYLIADNYNPSIEISNGFVTKIIPKHTWAVFAC
                     KGAMPKTLQDVNRKIFSEWLPNCSDYEIAAGYNIEMYTNTADYPQGNQDENYYSEIWIP
                     VKKK"
     gene            complement(18818..19891)
                     /locus_tag="LL065_RS23910"
                     /old_locus_tag="LL065_23890"
     CDS             complement(18818..19891)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444755.1"
                     /locus_tag="LL065_RS23910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_23890"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_226139108.1"
                     /transl_table=11
                     /translation="MKNLNIGDTILRCRKEKGITQEQLSNMVGISAGAVCKWETEKSFP
                     DIELLAPLARALNISLDELLSFNSKLLEVDVLNIKKRLTEVFIQEGYDAGYEKCKALLN
                     EYPNSVYLKLTIAELIQMYSMMYADKSEKLFKPKMECALKLLDQIVTSKDAKYMSKALF
                     SIASIQMMLENYDESEKALKELSTSSSIDPMTIYPMLLQRQGKNDEAKVQCENMLLSHI
                     IQSTSMLATIANISISTQNYENAQIYIDAIRKIQSLFNVGLHSGDYNLCKLYIKKGQLD
                     CAAKYFKIYVEGVISTEYDYSNNKFFKDVKLEVAAEGQKSVRKKTYQSYIDDSALNVLK
                     GLKDYEEGIRLLKANLY"
ORIGIN
        1 atatcaattg gcatatagta aaagcgccta taactggaat gttttcttat cgtataaaag
       61 aaggtagcag tcaatattgg gcagctattc aagtaagaaa tcataaatat cctgttgtga
      121 aaatggaatt ttcaaaaaat aatacttggg ttaatatgag aaaaatgcca tataaccatt
      181 tccttagtga gaatatgggg acaggctcta ttaagataag aataacggat atccgtggaa
      241 tatccgttac agacacaata tcagctttac ctaaaagcgg agattctccc gcttatatta
      301 ttcctggaca tgttcaacta ccagattaaa ttaaaattta aatgaaggtg ggtaaaatta
      361 tgaaaaaaat tataaaagtt ggaatgtcaa ttttattatt tactgcagtg ttaacagcat
      421 ttggagccaa taataaagca aatgctgctg caatatggtc atcatcacaa aaatatgggt
      481 catggcctaa tggtggatat acattaaata atgacgtatg gtcacaagat acagtaggac
      541 ctcaaactat ttgggctaac tcatatagta attggggaac atggtcagca cagcccaata
      601 cgggaggaat aaaatcctac ccccatgttg aaaaagcaat aaacaaaaag ttaagtactg
      661 taaagacagc tacaagtagc ttcaatgtta cagttcctac aagtggaact tctatggaaa
      721 ctgcctatga tatatggtta tctgatccta atgcttatga tcctactcat cctagtaaga
      781 ataagcatga aattatgtta tggatgaacc aatatggagc agtaaatcca atttcatata
      841 agtgggattc agcaggtcct attccagtgt ataaaaatgt atctatagga ggtcatacat
      901 ggaatgtata tataggaaac aatggattaa ataatacagg aaatatggtt tactcattta
      961 taagaactaa tggaaatatt agttcagggt ctctagatat taaggcaata gctaactgga
     1021 ttaagaatac accaaaatgg tatggagata ttttattcga taatatacaa tttggatatg
     1081 agattacatc aagttataat ggtggtaaag gttataattt taatacaaat actttttctg
     1141 taatatttaa ttagagaaat gaaaaaaact acaactgagt tatatttaat ttagaagtga
     1201 acgtgtgaga acataagaaa tatttcgcac acctaagttt acacaattaa ctagtacact
     1261 gttatttgtt ataaaaatag aaatatgagt gcttataaat ctaattaaat atagaaggag
     1321 ttttatatga ttaagaaatt atcaaatggt gaattggatc taaatgtcct agaaatcaaa
     1381 gatattattg atattaagtt actgcaaaca tttcaagaca attttgccac tgcacttact
     1441 atgtcaagtg ttactgtaga taaagatggc attgctgtaa ctaaaccaag ttcctataca
     1501 agggcctgtt taagctttac acaatcaact aaaataggcc atgagcgttg tgcattatca
     1561 cacaaaaaat gtggagaaga ggctacaaaa acaggtaaac catatattca tacttgtcat
     1621 gctggattat tagattttgc tgcgcctata tcggtaggtg attatcatat aggaagtatt
     1681 ttaggtggtc aagtttttac tgcagctcca tcagaagccg aatttaggaa aacagcgaat
     1741 gaaattggtg ttaatgaaga tgattatgta aaagcaatta atgaaattca cataactcat
     1801 gagaaaaatg taatagctgc tgctgaggtg ctctcaatag taacaaattc actttcacag
     1861 gctggctatg aaaaacttaa gttgagtata atgtctcaaa ctcttgcaga taattttagc
     1921 cagatatctg caactatgga agaactcagc gcttcctcca taacagtaac aaataaccaa
     1981 cataacttaa gtgaagagat tattaacgta aagaacatat ctatagagat aaattcaata
     2041 ttggattcta taaaaagtat agcagatcaa acaaagatgc tcggattaaa tgctgctata
     2101 gaagctgcta gggcaggaga ggctggtaga ggctttggtg tagttgcaac tgaaataaga
     2161 gctttatcac aaaattcaaa ggaaactgct gtaaaaatac tatcccttac aactaaaata
     2221 caaaactctg ttgataagac tattgaaact tctcatgcta cacttgagac tacagagcaa
     2281 caatcagctg caattcaaga ggttacttct actcttatgg aagttacaac tttagctgat
     2341 gaattaaatg caatggcaaa tgataattag catttaacat aataatgttt ttattgattg
     2401 ttttattaaa atatagtaaa tataggaata aaaaatatag gtgcgagtat atagcaccta
     2461 tatttttatg catatggatt atctttaaaa tttttgagct ataaataaag ggtgttatag
     2521 gttactttac atataatgtt gagaaaagca gtggattatc ggttctccag gatacattat
     2581 cagaaagtcc ccaccatgta atgcctgtga tatttgcgcc tgctttttta acctcacaga
     2641 ttgctttcat aatattatat gaatatgctg cctgttccgt ttccgtagtt gcacctgcat
     2701 ctagttctgt aatctgaatc tctaaccctg cattcataaa tgcatataaa gctgacttgt
     2761 aatagtcaac acttgggaat gaagtactga ggtgagattg gcaaccgata ccattacaat
     2821 aacgagtacc tgagttaata taattaatca tttttatgat attagaggat tccatgtatt
     2881 cattatagtc attgtagaat agacttactt tgtttgtcaa accaaaataa cttgttgttt
     2941 cgtaagcata acggaatgca ttttttacaa attgaggaga agttgtaaca tttccataaa
     3001 tcttaaccca tcctgatgtg gatggggaat gtaagtactc atttacaaca tcccatgcat
     3061 aaacaacact gccgtaattg cttgaatgaa catgattcat aactgtcttt atataaaatt
     3121 ccatacgtgc atccataaca gttgaactta cgtagcttcc gcttgagctg tatcttgtat
     3181 taaataagca agatggagtc tgcgagtgcc atattagagt atgttcacgg acacccaaac
     3241 cgttcttgta gcaaatttgt aatgccttgt caacagcagt aaaatcaatc tttggtactg
     3301 tagtctctgt ataattactt ggaatatagt atcctaatgc ttttgcttgt gaaaccgtaa
     3361 gcaaagtcgg agataaacca agtaatgcat ccggcttcat ttcattttca agggtaatgc
     3421 tgttgtatct ttgtttaatc acacttaaag tagccggatc ctgtaactgg gctaatgtta
     3481 cacaggaacc aactcttccg aagttacctc catacgtatt taacaaagtc tggttagtgg
     3541 ttgctgcttt agcctgcaaa actccaaacg atgaaatagc aattgcgatt actaatatta
     3601 ccattcttag atgtttcaat aattttttca taaaataata tcccgtataa tatttatata
     3661 tttaacaaaa gatttattct ttgaaataag ttgtcataag ctgcatgtgt tcatcactca
     3721 tcaacaaatt tgtcctattc cataaatatt ttcattttgt taaaatccaa ttatagattg
     3781 tggctttgcc tattattgtt gttatcacta atttataaac gtttacaatt atttaatgtt
     3841 attaaacaat agatcacgta gtgctgatct tgttaatata ctttttgatt gctcataaat
     3901 tgttcttttt tgttcaggat ctgtttcaag tttttttctc cagctagttt tagttcgtgc
     3961 catttttatt gaatttctaa tattagcgta ggttccctta ttaattttat aatttattaa
     4021 tatctgaacc acctcactaa tattttttta taatttttca taaatagtct ccttttaatt
     4081 atatgtttta tattatctta taaaaaaaca caataaaata caacagaaaa agcatgaaaa
     4141 gcactttttg aaacatgaac agtacttttg aaaacataaa atgcaaattt aaactatatt
     4201 tacctaatgt atcaataaat ttagttgcat ttttattatt tctacatact ataaattaca
     4261 attaactatg tgtaaattta tgtataatca agataaatga tcaagtacgg agtgaaacat
     4321 taccaataga agaatataat tattgcattt aacataaaat aatacctatt aggaaagttt
     4381 ggctgtggca tatagaacat aacggttacc aacgctatta atttatgaaa tacacttctt
     4441 gtacagtcga catacaggta agaacaaaca tatgccggaa tcaatacccc atatgtagtt
     4501 tttaaagcgt ttcatttgaa aattacaata aataaggttt tgaagtatac aaatagacca
     4561 aaagataaga gaggtgtata aaatctctct tatctaacat ggttactttc tgccaacaaa
     4621 caatttcatt agcatatcac taactaatat tgctgtattt gcaagttgtc ccttgtaaat
     4681 acaaatgctt gcgataatac ctatgaatac ggtagaaagc actatattta aaaaaggaag
     4741 aaagtgtcta ttatcaataa aactaataat aaacttgtgg ttttcttttg cttctaataa
     4801 gattaattca tttgtttttg ttttcggttt tgagtttgat tttcttgttg acataatata
     4861 cactccttct cttaaatatt ttttgacgat gcattggtca tatgtatcgt caattaaatt
     4921 tttaaaagaa ctaaggtagc aaagcatgac caatgctatc gctaccttag caaggtatgc
     4981 ttttgtcaac aagaaatcaa ccgaaacaat ttgtttatat cacatctgca aattatggtc
     5041 tcaaaaaact ctccatatta tcattaacat ttcctcttta aggttacttt aaattattat
     5101 aatgatatgg attactatac atattaatta tacatctatg ttaaaatttt acaatacttt
     5161 taaatacaaa gttccttgtt ttagtatatg cttttatatc ctctatattt ccacatttac
     5221 atatttattt gactatactc tacccattct gtaattaatt ttatatacct tatctatact
     5281 ttaaatttta tatagaatcc acttgcaagt tgggcatttt tccctatatt cctaaaaaag
     5341 gtatatcgta tgttactatg ttgaagtacg gtcatcactg tagtaactta aaagtaatat
     5401 attacataac aatatcaaaa tacaaaaagt agttgttaaa taagtaaaat agcctataaa
     5461 gtagactgtt ttaataggtc taccatatag actattgtat tattagaaaa atgctgacga
     5521 cacctctata aagcatatat acttctacac acacctggag ttttgttgtc tacgcctcca
     5581 actttaaatc cattaccgtc accttttatt ccgccgcagt taccattgtt tatagcttta
     5641 catcttgtaa atgttacagc cccatgagca gcatataaat catatccatc atctgaattt
     5701 ccctcagcgg tacaatctat taatttattt tcagcacctg aatgtaattt aattgcaaat
     5761 ccatcagtat tttcgccttt tgcatctgca ttactatagg agtaaacgtt agttaaagta
     5821 ttactagctg ctccatttga taattgtaca ccagcgtcat ggttaccatg aacctttaag
     5881 tttgttaaac gattacttga accttccata tatattccat tatctgcagc gccataaatt
     5941 tccaagtccg taattgtaga tccactagtg ttaattgttt atttttgggt ggttgtcgtt
     6001 gagtacgtgt ttaactaaaa aactactttg ttaaaatttt agaatgtaat ataacattct
     6061 taattcattc cataaaaaaa gaaggcgtta acctttataa tggtacctat aggaaggaca
     6121 cttgaaaaaa aagtgtttgg attataggtg ctttttttgt ataatttata tatagatagg
     6181 atggtggcaa atgagtaaaa ttttatttac caaaaaagat attataaagc ttgaaaaaaa
     6241 taaaaatgtc aaaaatgtta gtgtatgtgg aattacgtat gattttaaat ttaaaatgtt
     6301 atttataaat gaatatattg ctggaaaact accaaggcag atctttgccg aaaatggatt
     6361 tgatatagat gtaatagggt taaaaagaat cgagcaatca gcatataggt ggaattcagc
     6421 gtacaaaaag gatggaataa taggtttaga agacacaagg aaaacagcaa gcggtagacc
     6481 acgttctacc gagcttacta aggaagaaat aatagtgagg caaaatgcta gaataaactt
     6541 gcttgaggca caggtagaat tgttaaaaaa attagatgcg aaagaaaggc aggtggtaca
     6601 aaagaataag aagattaaat caagcgatgc ttttaaaact ataaaagaag ttattgaaaa
     6661 atatagcttt gtaaatgtag ttggatactt atgcgaatta tcaggagtgt ctagatctgg
     6721 ttactacaat tacattgctt caaaggatat acgggtagaa cgcgagcagc gtgatttaga
     6781 ggatagggac ttaatactaa aggcgtttaa tcgaaggggc tataagaaag gatctagatc
     6841 tattaagatg atattagtta atgaatttaa tattacatat agtttaaaac gaatccgtag
     6901 gattatgaat aaatacgaaa taatatgtcc tcacagaaag gcaaatccat atagaagaat
     6961 tgctaaagca acaaaagagc atagcgtagt aaaaaatatt ttgaatcgtg aattcaagca
     7021 ggctacgcct gaaaaggtac tgcttacaga tataacatat ttaccgtacg gcaatagtaa
     7081 aatggcctac ttatccacaa ttaaggactc ctcaacaaat gaaatattag catatcaaac
     7141 ttcagataga ataacacttg atatagcatt agatacaata aataagctag ttaaaaacag
     7201 acgagtaacg ttaacaaaag atgcttttat tcattcagat caaggtagcc attatacaag
     7261 cccaacattt caaaagctac taaaaaagca caaattatca cagtcaatgt ctcgtagagg
     7321 caactgttgg gacaacgcac cccaggaatc gttttttgga catatgaaag atgagataga
     7381 tttaaagtcc tgtactactc ttgagcatgt taagaaaatg atagataggt atattaaata
     7441 ctataataat tatagatacc agtggaactt gaaaaagatg actcctgtgc aatacagaaa
     7501 tcacctttta gcatcctagc actctttttt atactgtcct tgacataggt accattttac
     7561 ttctcttaca tttcatatta ttttatgtgt ctatataatg tggctctact tatattcaat
     7621 agttttgtaa attcgatacc tactacttct ttatttctca acttacttaa ttccaaaaaa
     7681 ctacaatttt atttgttgtt tcatctacat atgtcatata ccttatagaa ctttcaaact
     7741 gacttctaga catatttata aatttagaag tatcccatgg atttattact actacagtgt
     7801 ccttagttac actctttatt gaataggagt gacctccgtc ccctataatc cctttacttg
     7861 gatcagctat actaaaatca catgcagctg taataccatc tccagactta cattttaaag
     7921 ccatagtatt catcatattc gctttagcag tagttccact aactatattt tggccaaatc
     7981 ctgtcatttt agcaaataca tcacaagcaa aacaaccatt ataaacattc atggcttttt
     8041 gcatcccagc ttctataact ctagcatcaa ggtctccgct tatgtatgca ttggctacat
     8101 ctgtttccga tactgtctgt tttccggaac cgaaattgag tgtataacta ccatcactgt
     8161 taactgaaat cactttattt aagaattttt taccaaattt actattagct aatgcctgta
     8221 ttccagaaac agcaccgcaa tcattagcag ctccctggtg gaatgtgctt agtctcccat
     8281 caagttgcgt tgttggatca cccattgttc ctgcaaaagc agaaactggt gtaaatagag
     8341 tgcctgcaac cagtcctaaa gatagacatg aagtaataac tttcattttg atttttttca
     8401 tttttaaatc ccccttcgtt ttattatata aatccttaca ttagtaaggg cttattcagt
     8461 ttttataaaa aaccgaaaat agatggtatt tttaataatt taaaaaaatg taacgtttct
     8521 tgtttttatc ctcactctaa aatatttgtt ttgtatttaa atacaagtgg gtactatctt
     8581 ctaaatcctt aatggttgta cctttgttaa tattaactta tttaaaaata caataaaata
     8641 caataaaata caataaaaaa agcatgaaca gtcatttttt gatcataaaa agaaggtgtt
     8701 aaccctctct tacatttgat attcttttat gtgtctatat aatgtggctc tgcttatatt
     8761 gaatagcttt gcaaattcaa ctcctgttgc ttctttattt tcccacttac tataatgctt
     8821 attgaactct ttatgtaaac ttctagtagg tctaacccaa cttaattcca ttgtctttag
     8881 cattctttaa tcctaattaa taatatagat aatcttgtta agaaggttaa atatacggct
     8941 gagactattt caacagcatc tgaagaacta aatacggtat ctaaaagtac taacgactta
     9001 agtgaaaata ttggagagct ataactgaaa ctataaatag tatattatat tatgtaatat
     9061 attacgtttg agtacaactg gatgctgtta ctgcatcata ctaacatacc ttttcgggtg
     9121 gataggcaaa aacatccaga tttaaaaggt actatttagt gacaaattga ccatatccaa
     9181 acaagattgt caaacatagt gcaatatcat agaacgttaa agtaccgaac cacacgagcc
     9241 gaattcatca gtagctgata ttgcttcaca cagagttttg aaaaaatgtg gaatgaaaaa
     9301 atataaggaa acagacgatg aaatttggtg gcgcatgaaa gttgctgaat aatataatct
     9361 ttagtcaaca tactacgtat tcttcatata ttatccacca acccaagatg cagcaatgct
     9421 tttttcttag attgatgata aacattgata ttagttagtt ttaggctatt ttttattctt
     9481 gttagtgtga ttcagagaaa tcttagcggg gccctcatat tgcaacgttc ataatttgtc
     9541 ttattttctt aactaatgtt tcaatgctaa aaggttttag gatataataa tcggctccta
     9601 gtgctagggc tttttgagtt gttgtttttt ttgcaactgc tgataaaact agtatatggg
     9661 gctttatatc aaaattcatt gtagttaacc tctctagaac ccccaaccca tccataatag
     9721 gcatattcat atcaagtatt actacatcag gctttttttc ttgaatcaaa tctaatgctt
     9781 ctactccatt agctgcgata cccgtaacaa caatatcctt ttgcattgat atataatcat
     9841 taagaatatt actaaagtct ttgttgtcat cagcaatgat tatgtttatt ttcgaccctt
     9901 ccatatttgt aatctccttt acttttaaat aatatataat actatttata aattcagaat
     9961 gttttttgta attatgactt tgtataataa acattataca ttaattgtca cttgagcaat
    10021 tcttatcttt attgttgttt atgcaaccca tcatcatcat cattacagct ataaaaatag
    10081 ctagaactgc tgtagtaaaa aattcatctg gtataatata acttattcca ctcaataaag
    10141 aagttataac aacagataaa tatttgagtt tatttcttct ctctttactt tttattcttt
    10201 tattaatatt atcaacagga gaaaaaatat atacaagcaa aatagaaata attaatacta
    10261 ctatcgaagt gagaatattt attgttatat atttaccgat aataatcatt acacaaaaag
    10321 ttacaaaact aataaaaaaa catccccaat atgtttttgc atgaaaacca cctgaaaata
    10381 atcttattgg acaaaaaaac acaaaaataa ctatatagta ttgttgaagt gaaaataaat
    10441 agaatattat ataatacgga attatcttag taatttcatc aaataaacag atgaatccat
    10501 agttcatttt ttttagttct agttccgtaa attgagaatt ggtttcagaa atctttttca
    10561 ctaaataact tactaattta ttcattcatt taaccttcct ctacattaaa aattcaatta
    10621 aaaatttagt ttttaatgaa gtcctttcta cagatatact tccattatta tgctcgacta
    10681 gttgtttagt aatatataat ccatacccat gatcactttt tcccttagtt tttgtagaat
    10741 atcccttttc aaaaatttta tttattattt ctttaggaat accacttcca ttatttgata
    10801 tttcaatgca aaacttgtta tcatctaata aagtagtaat agatatttct ttattatcat
    10861 ccaacgcttt taacattaat gcctcaaacg cattatctat aatattgcta atgatactta
    10921 ttaactcatc ttccctaatt tttagcaaac taaaagattc attaataagt actttgaaat
    10981 ctatattatt ttgagtcgca tagttgtttt ttattgataa taaaccatct agataatcat
    11041 ttccagtatc taaatatcta aatgataagt gaattatgtc cgaaagtttt gatacatatt
    11101 cttttattcg ctcaacagta ttgggtttgt ttaaacaaca caatgcttgt ataacattta
    11161 tatgattagc ataatcatgt ttttcttgtc taataatatt aattatttcc tccatattag
    11221 aaatttgata ttgttgaact ttatatttat tgcttatatt tattaattcc acattttttt
    11281 tcaaatgcat aaatgcgatt aataaaaata taaaaaatga attgaaaatt aatataccat
    11341 atattttgtt atttttaata tctaaaacac tgaataataa aacaaataaa aataaactaa
    11401 agacccctag ttctataaaa aacttagaaa aaagagattt ttcgctatta aatagttctc
    11461 tcttattcaa aaatatatta tattttgaaa ttatgattaa aacaagtatc tgtagcaatt
    11521 tactaccaat tctaaaaata agtgcgtact tataaatcgt caaaatttca tttagttcaa
    11581 ctttaagtat aaacatttca ataaattgaa ttaaagtttc tgtaacaaat atcattgtaa
    11641 aaaataacga aaatattata atcgaggaat acacatttgt ttttgtaata aatgttaata
    11701 acactataga aactattatt gttactagag tatggtataa attatcatta aaataatttg
    11761 tacttaaata agctgtaata atgtatgaca tacaaaataa tccggatcta attttatttt
    11821 caacaatata cttcttttgg ttcgttaaaa ggctaaaagt tcccactaat aataatgcct
    11881 caaaaatatc aagtattatt tctataatca tttttatctt ctccttaata aaacaatgac
    11941 tcttttgtat taaaaattaa aactttcata caaaagagtc attattttta tcttaaaaat
    12001 tcatcagggc agttaggttc atatgcatga agaagtgatg caggcataat agctacattt
    12061 cccaaaagta atcctagaca tcccatagta gtaatggttt tacaaattag ttttttcaac
    12121 attagttcca cccctttttt aaattatata tcaagatttt tgtataaaat actattactt
    12181 gataaagtta tatattctac ataactttcc atattccttt attataacta catatttaca
    12241 attataataa ataattctat actttattgg tatatttaga aatatttcac ataatatttg
    12301 aatgtataaa gtaactagtc ctacgtcagt tggctcaacc tcataaagta tttgtcttct
    12361 atattttagc gcaaaagcta cgtgataaaa tattattgtc catttagaac atacctcctt
    12421 ttaaaatagt tgtgattgaa aaacttacta ctattataca aagagatgtt attttttcta
    12481 ataatatcta aaaattcaga aaagtctcta taattcttct ttacagatta tggtataaaa
    12541 atacctagca tatatgcagt ttgttaacgt tttaacgacg tcatattaca tttattgtat
    12601 ttatatcatt tttttagtta aattagtgat aatattctac ttacaaaata taaaaaatct
    12661 tattcattct aacatgttta tcataaaacg aattacgttt ttaatattaa ttaaatttta
    12721 taataagagg aggaagaaaa ttatgaacaa ggataatcta tatttaggag tgttcgaaga
    12781 cgttgaaatt gctatttcaa aagcaattca tgcacaaaag gtgttaacta ctgattataa
    12841 agaagaagat agagaaaggc ttataaacag tataagaaag gctacattaa aaaataaaga
    12901 aacgcttgca aaaatgatta tagaagaaac aaacatggga cgatacgaag ataaaatatt
    12961 aaaacatgaa ttagctgcta aatatactcc tggtacagaa gatttaacta ctacagcttg
    13021 gtctggagat caaggactta cagtagtaaa gatgtctcct tacggtgtta tcggcgcaat
    13081 aacaccctct acaaatccaa ctgaaactgt tatatgcaat agtataggta tgatagctgc
    13141 cggaaactct gtcgtgttta atggacatcc aggagcaaaa aaatgtgttg cttttgccgt
    13201 ggatatgata aatagagcaa tcctctcagc tggtggtcca gcaaatttag taactactgt
    13261 gaaaaatcca actatggaat ccttagatgt aattatgaaa cacccatcta taaaactttt
    13321 atgtgggact ggaggaccag gattaataaa aacgctttta aactcaggta aaaaagctat
    13381 aggtgcaggt tcaggaaatc ctccagttat agtagatgat actgcagata taaaaaaagc
    13441 tgccaaaagt ataatagacg gttgttcttt tgataataat ttaccttgta ttgcagaaaa
    13501 agaagtgttt gtttttgaaa atgtagcaaa tgatttaata aataatatgc ttcaaaacaa
    13561 ttctgtgctt ataaacaaag agcaagtatc aaagttatta gatttaatac taataaagaa
    13621 agatgaaaaa agttataatc acaacaaaaa accagaatat aatataaata aaaaatgggt
    13681 aggtaaagat gcaaaattat ttttagatca aatagatgta gaagccccta ctaacgttaa
    13741 atgtataata tgtgaggtag atataaatca cccttttgta atgacagagc ttatgatgcc
    13801 aatactctca attgtaagag ttaaagacat tgatgaagct atagagtgcg caaaaatagc
    13861 ggaacaacac aaaagacata gtgcatatat atattcaaaa aatatcgata atttaaatag
    13921 atttgaaaca gaaatagata caactatttt tgtaaagaat gctaaatcct ttgctggagt
    13981 aggttctggt gcagaaggat ttacaacttt cactattgct ggacctactg gtgagggtat
    14041 aacatctgca agagatttta caagacaaag gagatgtgta cttgtaagct aacttttaat
    14101 agagtaagtg actgcctaca tttccatcaa aaaaatttta tactgttaga aaggagctat
    14161 taaaatgaat aaaatatttg aaatggaaga attaaaatct atttttaaag atggaatgac
    14221 aattatgatc ggtggttttt tagattgtgg aaccccagac acaataattg atatgttggt
    14281 tgatttaaat ataaaagatt taaccatcat tgcaaatgat acagggttcc ccgataaagg
    14341 tatagggaaa ttggttgtga atggccaagt aaagaaagtg attgcttcac atattggaac
    14401 caatcctgag actggaagaa ggatgaatac tggtgagatg gaagtagaac tttctccaca
    14461 aggaacatta attgaaagaa tccgtgcagc tggctctggt ctaggcggtg tattaacacc
    14521 aactggacta ggaactattg tagaaaacgg aaaacgagta attacaatta gcaatactaa
    14581 ttatttatta gaacttccat taaaggcaga tgtatcactt gtaaaaggta gtatagtaga
    14641 tgaatttggg aatacctact ataaagcaac tacaaaaaat ttcaatcctt atattgcaat
    14701 ggctgcaaaa acggtaataa tggaagctga aagcttagta aaatgtgaac aattaagaac
    14761 agaagatata atgactcctg gtgtacttgt gaattatatt gtaaaggagg cagtttaatg
    14821 attacagata gtaagttagc aaaagaaatt attgctaaaa gagttgcaaa agaattaaaa
    14881 gatggtcaac ttgtaaatct tggtatagga cttccaactt tggtgggaaa ttatgtgcca
    14941 aagggaatgc atattacttt tcaatcagaa aatggaatgg ttggcatggg tccaaaagca
    15001 aaaccagggc aagaagatac aaatttaatt aatgcaggtg gacagtgtgt aactgtactt
    15061 ccagagggtt cattctttga tagttctatg tcatttgcat taattagagg tggacatgtt
    15121 gatgttactg tacttggggc tttagaagta gatgaaaaag gaaatttagc taattggatt
    15181 gttccaggca aaatggtacc tggaatgggc ggtgctatgg atttagtaat gggtgcaaat
    15241 gtagtaatta tagccatgcg acatactgga aaaggaaaac ctaaaattct taaaaaatgt
    15301 acactgccac tcactgcaaa atctcaagta aatttaattg ttacagaact ttgtgtaatt
    15361 gaagtaacag ctaatggact acttttaaaa gaaattcata aagatacaac aattgatgaa
    15421 attaaatctg taacagaagc aactttaatt attccagata atataaaaat tatggatgta
    15481 taaaaatcta tcattaatta aaaaataaga aaagagagtg aatattttat gttagaaagt
    15541 gaaatatcta aacaaattac aaccccactt actgccccag cattcccaaa aggaccatat
    15601 aagttccata atagggaata cttaaatata ctataccgta ctgacctagc gtcacttcgg
    15661 aaaatggtgc cagaaccatt agaattagat gaggatgagc cactagttag atttgaaatg
    15721 atggctatgc cagatgttac tggattggga tcctacactg aatgtgggca ggttattcca
    15781 gtaaagtata atgggattaa aggtgattat ctgcatatga tgtacttaga caatcaccca
    15841 gccattgctg ttggaagaga gattagtgct tatcctaaga aactgggtac tccaaaacta
    15901 tttgttgact cagatacatt agttggtaca ctaaactatg gctcattacg agttgccact
    15961 gctacaatgg gttataaaca tgaacctcta gatattaagg aagcttataa ccaaatttct
    16021 cgtcctagct ttatgctgaa aattttacaa ggctacgatg gaaaaccaag aatttgtgaa
    16081 attatatgta cacagaataa ggatattacc atacacggtg catggactgg ctgtgcacgt
    16141 ctgcaattat tcagtcacgc tttagctcct ttagctgatt tacaagtctt agaagttgta
    16201 tctgcatcac atattcttac agatttaact cttgcatcac ctcatgttgt gtacgattat
    16261 cttgcaaaga attagagcat caagtaccat tagatcttct tgcaatgaat tcacaaaatg
    16321 aacaagttca tgtaacttct tgccaaggat tctttgatga cgaaatttca agtaggtatt
    16381 atgcaattac acttgtgtta cattcacttt attttttaat cccctttttc agaaaaagat
    16441 aggttatata atacgctact attagcataa ttaatccaaa gagatttgga atcattatat
    16501 taatattttg gaatatgttt tcataatgca ataagggtgt taaataaatt gcattacaca
    16561 tagatattat caatccaatg gtaggtatta taatatctac cttttttatt attactattt
    16621 ctgcaatgat gtaataaaaa attatagtac acatccaaaa acttatcaca agccacgcgt
    16681 gagataacct aattgcatat ttagttttaa aataagtaag agtcaaagtc aatataataa
    16741 ataaaactat tattggagca attttagtga atttcagcat tgtgtctaat ctttttttta
    16801 tctttaaatt aattttgtca gaatatttat aaaaaatatt ttttgatgat atcattaaaa
    16861 aaattaataa taaactaaat cctaaaatta agtatgacaa agtcatcccc ctttttacta
    16921 cgcaataatt tacatgatag tattttacta attatgattt aattaatatt ttaattaagt
    16981 tgaattttta gatttgaatt aattacttta aattggttac ttatcaattt tttatcatta
    17041 tttgaataac ccttcaaact caatcttatc tttttattat tcataagtgt aatttctatt
    17101 aaattgtccg atgtaagcag caattcagta atatttttaa tttgctgtaa atctattgta
    17161 taatatctca tcaaaaacca ctttttaata gctatatggt tgttatctat aattaaatct
    17221 gataatttat attttttagt aataattata tataattgat ttaaaattaa tacaataaaa
    17281 gtacctgcaa taaatagtcc atacagtctt tcgtagatag aatttgctgt aaaatccctg
    17341 atatgaaaca ggattggaaa tatacaaact gtataaatta aattattaaa tggttcagaa
    17401 ggttctcttt taaatgtata ttctttattt tcagacaaac taatcactct tctcccttat
    17461 ttactcacta taaagtacaa tttctactaa cactacatat ttgatcatac cataattatc
    17521 ataaattaaa tatatttact aatgatacta tagagaataa ataaagtacc cacatatcat
    17581 taaatatgtg ggtacttgcg acatataaaa gaaagataca acaaaaacta aatctaaaat
    17641 tattctacat caactttaca aattggaatt tgatgaactc cgaattgtgc tcctttacgt
    17701 agttattttt tcttaacagg aatccaaatt tcactgtagt agttttcatc ttgattacct
    17761 tgcggataat cagccgtatt ggtgtacatt tctatattat aacccgctgc aatttcataa
    17821 tcactgcaat taggaagcca ttctgagaag atttttctgt tcacatcttg cagagttttc
    17881 ggcattgcgc ctttgcaagc aaaaacagcc cacgtgtgtt taggaataat ctttgtaaca
    17941 aaaccattag atatttctat ggatggatta taattgtctg caatcaaata ttcgaactca
    18001 tctgaaccca tgctatcgtc tatgcatact ccgtacattc cgcatacaaa tttccctttt
    18061 cctgtttgat aatgctctgt ccaaaattgt ggaatttctg tagttgcatt atcatattta
    18121 aatacctttg atacgcctat gacagtaaac gaatcttttt taacaatttt gtaatccata
    18181 atataaccgc cttccaatga aaatttgatt ttcagcggag caaatgattt aatcattgct
    18241 ccatcttttc gaacagcagt aggtgtgaca ccatggaatc gagtaaaagc ctttgtgaaa
    18301 ctatctggtg aggcatagcc atatttcagt gcaatatcaa tgattttttc atcagtagaa
    18361 acaagctcac tgccggcaat ggtaagcctg cgttgtctaa tgtactctcc gaccgtaaaa
    18421 ccacaaagca ttgcaaagcc tttttggaaa tagaacggag acataagtac tttttttgca
    18481 acatttttta ttgcgatttc ttcagtgata ttctcctcaa tatagttgat agcttcaccg
    18541 atttcttcaa tccaacccat aatcatcccc cctgtctgta catatatcgt atctcttttc
    18601 aagcggttgt tcccgacttt ttgtgttatg ttttgtctta tctgaaagat tattcaataa
    18661 taccatattt actgtaagta ttaacatatt tacaactatt tttaaatccc tgatagcatc
    18721 aaattgaagt taatttaact ttggttttta tcattaggtt agtaggtagt ttacgaatat
    18781 tatggtttta ttatattgct tataagctta tttattttta ataaagatta gcttttagaa
    18841 gcctaatacc ttcctcataa tctttcaatc cttttaaaac gtttaatgcg gaatcatcta
    18901 tatatgattg gtacgttttc tttcttacgg atttttggcc ttccgcagct acctctaatt
    18961 taacatcttt gaaaaattta ttattactat aatcgtattc agtagaaatc acaccttcaa
    19021 cataaatttt aaaatatttt gccgcacaat ctaattgtcc ctttttaata tatagcttgc
    19081 aaagattgta gtcacctgaa tgcaacccca cattaaataa gctttgtatt ttacgaatag
    19141 catctatata tatttgtgca ttttcatagt tttgcgtact aatagatata ttcgctatgg
    19201 tagcaagcat tgaagtgctt tgaattatat gtgataaaag catattttca cattggactt
    19261 tagcttcatc atttttgcct tgacgttgca atagcattgg gtaaattgtc ataggatcta
    19321 tagatgatga ggttgaaagt tcttttaatg ctttttcgct ttcatcatag ttttctaaca
    19381 tcatttgaat actggcaata gaaaagagag cctttgacat atatttagca tctttgcttg
    19441 ttacaatttg gtctaataat ttaagagcac attccatttt gggtttaaag agtttctcgg
    19501 acttatctgc gtacatcatt gaatacattt gtataagttc agcaattgtc aacttgagat
    19561 acacgctatt agggtattca tttaaaagtg ctttgcactt ttcgtatcct gcatcatatc
    19621 cttcttgtat aaatacttct gtcaatcttt tcttaatgtt aagcacatca acttctagca
    19681 gtttagaatt aaaagacaat agttcatcta acgaaatatt taatgcacgt gcaagcggtg
    19741 caagtagttc aatatctgga aacgattttt cagtttccca tttacaaaca gcaccagccg
    19801 aaatccctac catatttgat agttgctctt gtgtaatacc tttttctttt ctacaacgca
    19861 atattgtatc accaatattt agatttttca tataaacctc catttgttaa atttaaaacc
    19921 ttttgatgat tttattataa gttaattcaa aaatccatac aatggaggct aaatcataat
    19981 aaggttaata aaaattgaat gctattcaat acaggagtac atcaaacgtt aaggaatata
    20041 tcaattaata aagttcgtgt tatatcatag caaaactcat tacatactct aatgtttctc
    20101 ccgctttagc attaacaagg actttatgat agtattaggc acaatgtatt atgaaaatca
    20161 cctttttatt aagtaagaga ttatattgag tttttctttt ttcgtatttt tttagcgcta
    20221 atatcaaact atattccccc aatctttaac atagtaatga acaatatccc tgccattgag
    20281 atgtaatatt ttataattgt gatacaactt ctatacttta ttgtaacata tatatccata
    20341 tcatattcca ttacttgtaa gggtccaaaa atctatagtt aaatgatacc cgactcctaa
    20401 ataaattaca aaaatgaaca agaattgata tgtattaatt ggtgatatat actaaatata
    20461 aatttaatat tgactctcac atggtgttag gcattattat aaagatgtca ttcgaatggc
    20521 agtgatttag aggagttgat tttatgttca agattggaga tttttccaag ttgaccagag
    20581 tgtcc
//
