LOCUS       NZ_CP086140            20831 bp    DNA     circular CON 20-JAN-2024
DEFINITION  Clostridium estertheticum strain CF016 chromosome, complete genome.
ACCESSION   NZ_CP086140
VERSION     NZ_CP086140.1
KEYWORDS    RefSeq.
SOURCE      Clostridium estertheticum
  ORGANISM  Clostridium estertheticum
            Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5053333)
  AUTHORS   Wambui,J., Stevens,M.J.A. and Stephan,R.
  TITLE     Clostridia strains as spoilage organisms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053333)
  AUTHORS   Wambui,J., Stephan,R. and Stevens,M.J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2021
            Assembly Method                   :: unicycler v. 1
            Assembly Name                     :: CF016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Nanopore/Illumina miniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 05:10:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,249
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 5,019
            CDSs (with protein)               :: 5,019
            Genes (RNA)                       :: 135
            rRNAs                             :: 15, 14, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 13 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 22 of 95
            Pseudo Genes (incomplete)         :: 78 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:27:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4409338
            Orig. end                         :: 4430169
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086140.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20831
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="9"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20831
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20831
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10831](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10831
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            complement(321..998)
                     /locus_tag="LL065_RS20880"
                     /old_locus_tag="LL065_20865"
     CDS             complement(321..998)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1188: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894786.1"
                     /locus_tag="LL065_RS20880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20865"
                     /product="DedA family protein"
                     /protein_id="WP_226136652.1"
                     /transl_table=11
                     /translation="MDILINFINVIMHIDKYLTLMVQQYGMLTYGVLFLIIFIETGLVI
                     TPFLPGDSMLFAAGALAGIGSMNIFTLLIISYLAAILGDTANYFIGKKIGKKILAKEKV
                     RFINKEYLIRAQEFYEKHGSMTIVIARFIPIIRTFAPFVAGIGKMNYSKFVPYNIVGGG
                     LWVSLFLGGGYFFGNLSFVKTHFSYMLVAIIIISLLPGVIVFIKEKRKKNDDNEDEVQG
                     TEI"
     gene            complement(1138..2451)
                     /locus_tag="LL065_RS20885"
                     /old_locus_tag="LL065_20870"
     CDS             complement(1138..2451)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233672.1"
                     /locus_tag="LL065_RS20885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20870"
                     /product="VTT domain-containing protein"
                     /protein_id="WP_226136651.1"
                     /transl_table=11
                     /translation="MHSVILLINNYGYIILFSALVLELIAFPLPGELMMTYCGFLVYQS
                     KMNWGISILVATAGVMLGITISYFVGTKLGVNFFEKYGSYIHLGPAKIEKTSKWFNTSG
                     NKLLILAYFIPGVRHITGYFSGITKISYVRFASNAYFGAFLWTTTFISLGKVLGPNWHK
                     FHGYISKYLIIGSIIISLILIIIYAYRNHKLQIIENTYKVLNKTIIIVHSMGKMKVAIG
                     AVAVAFLGFSVLIIGVVQDYLSHEFDQFDIVVSYLVKVFFNSKWSFVIGLFKLTTSIRV
                     LVPLTIFMFIWIIRRNINRILEIQSLFIVIFGGEILQYVLRHIFRRLGPSSIRLISNNQ
                     YTFPSYQALMAIVVYGFFVYLIVRHAKKVWSKTVVFIMTLFICAISGLNPIFFGMEYPS
                     DVYAGYIFGGVWLTINIILLEVYRILPTIKFKNSNFLK"
     gene            complement(2487..3035)
                     /locus_tag="LL065_RS20890"
                     /old_locus_tag="LL065_20875"
     CDS             complement(2487..3035)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239854.1"
                     /locus_tag="LL065_RS20890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20875"
                     /product="phosphatase PAP2 family protein"
                     /protein_id="WP_226136650.1"
                     /transl_table=11
                     /translation="MMYLLQKFDISILLFIKYNMHGIIMDKVMVISSYLGNAGVIWIII
                     ASILISNKKYRKIGFMALAALILSAILGDEILKNIFKRLRPSDTIPTMNLLITRPLSYS
                     FPSGHSMSSFAVAGVLAKYFKKYVIEIISLASLIAFSRVYLYVHYPTDVLVGAILGLVC
                     SALVIYIFDNIKNNSKASI"
     gene            complement(3159..4568)
                     /locus_tag="LL065_RS20895"
                     /old_locus_tag="LL065_20880"
     CDS             complement(3159..4568)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238242.1"
                     /locus_tag="LL065_RS20895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20880"
                     /product="ATP-binding protein"
                     /protein_id="WP_226136649.1"
                     /transl_table=11
                     /translation="MNFKIMGPKIMNLKMIKSAKISLKLTVIYAVMFSLILLILNASIL
                     YGIKYYLYGQANKQIEDVKTIVLNNIVSRNEHGDLSDKELISDVTSEQNISIRILKQDG
                     KILNKSKEFNYNIISKEPFDKIRKIKEDDKHLVYKILKIETQKYGVIYIQIVKWMCNEY
                     DFMQILFILMAIADFIGMIASIILGYIISKKMLEPIDQITKAADNISINNLLGRIEVTG
                     PDDELKRLGITFNKMIDRLQESFDRQIQFVSDASHELRTPIAVIQGYANLIDRWGKDDR
                     EALEKSIHAIKLETSNMANLVEKLLFIAKGSSGNQRIEKGDFLLNDLICEVISETKLID
                     HSHIISSYKNDSISIFADYKMMKQMLRILIDNSIKFTPEKGTIDISSEIYCNTVKITVS
                     DSGMGIPEDEIQNVFERFYTVDKSRSKEKGGTGLGLSIAKLIVDVHKGTISAESTEGFG
                     TKITVMLNINY"
     misc_feature    complement(4526..4540)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.46"
                     /tool="antismash"
     gene            complement(4571..5251)
                     /locus_tag="LL065_RS20900"
                     /old_locus_tag="LL065_20885"
     CDS             complement(4571..5251)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425358.1"
                     /locus_tag="LL065_RS20900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20885"
                     /product="response regulator transcription factor"
                     /protein_id="WP_226131747.1"
                     /transl_table=11
                     /translation="MKDFRILLVEDERLMSTFIEMELSHEGYKVDVAYDGREGLTIAEE
                     NEYNLILLDVMLPGLNGIEVCRRIRQLSLVPVIMLTAKSDISDKVLGLDVGANDYLTKP
                     FAIEELLARIRVYQRDTSIGNKSDEIKVKDIVMDNKAHNVKRGSKEIELTKKEFDLLEM
                     LLINKNVVLTRVQLIEKVWGYDYIGDTNVVDVFIRYLRCKIDDGFEDKLITTVRGVGYV
                     VKGE"
     gene            complement(5474..5797)
                     /locus_tag="LL065_RS20905"
                     /old_locus_tag="LL065_20890"
     CDS             complement(5474..5797)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS20905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_20890"
                     /product="hypothetical protein"
                     /protein_id="WP_226136648.1"
                     /transl_table=11
                     /translation="MGEKDLEKPEKNRNTIEITIPFNGNISGANVGSNSSDDDTSEKKQ
                     KSDQSDNDNSKEGQMSSKADSGDCSNGDPAQKEKKSNKSEESNISVDATSKKGQMIINI
                     SEK"
     gene            6020..6700
                     /locus_tag="LL065_RS20910"
                     /old_locus_tag="LL065_20895"
     CDS             6020..6700
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987950.1"
                     /locus_tag="LL065_RS20910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20895"
                     /product="response regulator transcription factor"
                     /protein_id="WP_226136647.1"
                     /transl_table=11
                     /translation="MNNILIIEDEEKISEILKAYLEKEGYSVYTTTKGLDAIRLFEKIQ
                     FNLVILDLMLPDISGEEVCSILRKISEVHIFMLTAKSTLEDRIEGLNLGADEYLIKPFS
                     PRELTARVNALFRRININKSETMVYDNGDLEINYDKRVVFVRGEEISLTPNEFDILQIL
                     SSNGGKVFTREHLIERIMGIDFQGFDRTIDVHIKNIRKKIELDSRNPKYIITVTRIGYK
                     FGGD"
     gene            6704..8071
                     /locus_tag="LL065_RS20915"
                     /old_locus_tag="LL065_20900"
     CDS             6704..8071
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233956.1"
                     /locus_tag="LL065_RS20915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20900"
                     /product="ATP-binding protein"
                     /protein_id="WP_226136646.1"
                     /transl_table=11
                     /translation="MQSIRKRLSIILVCTSILALFLATLFVNIAISKTFNKYLVEIQTK
                     KDTGIVKYLETVYEKNGKWSKTSGIEITHEAYMSNYCLTLKDINKKVVWGMNANDIINN
                     TNTTMPHKSNGVYTLRTLEIKYKDKIIGYVDIGQNGPVLLTSDDITFKTSINKGIILSI
                     VLSLLITILISLLVSKQFSNPLRQVSKISVDLSHGNYRARSYDKSTILEFENLRNSINT
                     LGENLQNQDAIRKRLISDISHEVRTPLNILQNNFEAMIDGIFPADNERLTYLNDEVIRF
                     GKLIDNLNTLKQIEGEETALNMEIISLKDILTLVKKEFELLSENKNITMTLKCSTNNQC
                     DILGDTDKIKQVIINLLSNAVKFTQNQGEINITLKESNKKIMLKIQDTGIGIKEEDLPY
                     IFERLYRGDKSRHEIEGSGIGLTIVRNILTLHKADIEVKSTEGKGSTFILYFNKVL"
     misc_feature    8157..8172
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.73"
                     /tool="antismash"
     gene            8239..8487
                     /locus_tag="LL065_RS20920"
                     /old_locus_tag="LL065_20905"
     CDS             8239..8487
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059146.1"
                     /locus_tag="LL065_RS20920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20905"
                     /product="SHOCT domain-containing protein"
                     /protein_id="WP_226136645.1"
                     /transl_table=11
                     /translation="MYCNRFLGSGYGTGVGMFLMMVFGLLIFSAFVFLVFKLMKVNSPS
                     SFSSSNLALDILNERYAKGEINEEEYTKKNTILSRKN"
     gene            complement(8697..>9038)
                     /locus_tag="LL065_RS20925"
                     /old_locus_tag="LL065_20910"
                     /pseudo=""
     CDS             complement(8697..>9038)
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443692.1"
                     /locus_tag="LL065_RS20925"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20910"
                     /product="C40 family peptidase"
                     /pseudo=""
                     /transl_table=11
                     /translation="AIIAYASNFLGTPYLWGGTTPSGFDCSGFTQYVYAHFGISVGRTT
                     FDQINDGVEVSRDNLQPGDLVFFGTFANPHHMGMYIGDNNYIHAPHTGDVIKISALGRN
                     DYVTARRVK"
     misc_feature    complement(9254..9269)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            complement(9292..9423)
                     /locus_tag="LL065_RS20930"
     CDS             complement(9292..9423)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS20930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_264174731.1"
                     /transl_table=11
                     /translation="MTKTEENKGKISETEKSIEVAAVDVKKVAKESQKEQGLFNSRK"
     gene            complement(9572..9703)
                     /locus_tag="LL065_RS20935"
                     /old_locus_tag="LL065_20915"
     CDS             complement(9572..9703)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170698.1"
                     /locus_tag="LL065_RS20935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20915"
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="WP_216123742.1"
                     /transl_table=11
                     /translation="MWIEVSIALFIIAIAGHIIYHSLKSKGSGDCGCGNCSSKQKKK"
     misc_feature    9933..9949
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            10001..10831
                     /locus_tag="LL065_RS20940"
                     /old_locus_tag="LL065_20920"
     CDS             10001..10831
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234002.1"
                     /locus_tag="LL065_RS20940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20920"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_268064576.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 2.2e-37, bitscore:
                     126.8, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENLQFKDIYCPISKDLNLVEKELRKTCENLSTNYLQEIIGYFFK
                     IPGKRLRPTLALLSSGIINNNLPESTNNQLIKYATGLELMHSASLIHDDVLDNDMVRRG
                     QKTLNEIYGNKIAVLAGDVVYSLAFSTLSSSLPKEFKQTIVELTENMCASEVIQAENML
                     PTREIYLDIIRGKTALFMSVSCKIAAALAGATKEQVNSIEEYGLNLGMTYQIMDDCMDK
                     DVYAELNITSEDAKLYGNIAISSLKTFEDSAYKISLINLVNFILSLSHTKVNNS"
     gene            10966..11502
                     /locus_tag="LL065_RS20945"
                     /old_locus_tag="LL065_20925"
     CDS             10966..11502
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /locus_tag="LL065_RS20945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20925"
                     /product="FixH family protein"
                     /protein_id="WP_226136643.1"
                     /transl_table=11
                     /translation="MKIKLFKNLVFTLILALSFSTLAFASTTGTQVNKTVDGVKASLTF
                     ATEKLKPGSNEFTISILDKNGKPLPNANLKVTTNMDNSTNMTGMSGMSTDNKPMMIVLK
                     QRSMKGEYTGMVDFTSTGKWIVKSTFNVQGQAKNIYFNVNVQKSGPNFLIIGFAGVMVL
                     IIVVAAINKKKSIKS"
     gene            11522..12916
                     /locus_tag="LL065_RS20950"
                     /old_locus_tag="LL065_20930"
     CDS             11522..12916
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423039.1"
                     /locus_tag="LL065_RS20950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20930"
                     /product="4Fe-4S binding protein"
                     /protein_id="WP_226136642.1"
                     /transl_table=11
                     /translation="MANIKTPLKPDLSTTTEKKHKDFLKNKTFKKFIKSKWYPGIFQWP
                     IALVFAFIVYELIAGPTDAHDNFGTAGTWVLWWPLLPILLLLFGRFWCILCPFGLLNDV
                     VQKFVGNNKPVPKFLRKYGIWIIDATFLMITWSDHVWGVVENPRGSGVLLLMITTAVIF
                     SGALFERRTWCRYLCFLGGLSGNYSRAGMVELRATPEVCKNCKTASCYKGTTEVPGCPM
                     FEFPKTMESSAECNLCGNCVKNCPNDSIRISPRVPTEELWDIKKPRVEVAFLAIVIMGI
                     VFVQNITMLTIWEKILHSLENILGTTNYYITFTVTFIIAMLIPVVLLLLTGLIAKKFNK
                     ASVMENFAKFGYAIIPLDLAGHIAHNLFHLLAEGKSVIYTIMELFGVSSHGASTAILPS
                     PSIQILQYIIIVIGTVGSIYTAYRIAKSSQTKTTLLGTSVVYAILMVLLAIANIILFSI
                     PMAMRM"
     gene            12947..13654
                     /gene="ubiE"
                     /locus_tag="LL065_RS20955"
                     /old_locus_tag="LL065_20935"
     CDS             12947..13654
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /GO_function="GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="ubiE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615181.1"
                     /locus_tag="LL065_RS20955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20935"
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="WP_226136641.1"
                     /sec_met_domain="PCMT (E-value: 3.1e-07, bitscore: 28.4,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSETITDVQGIFSSIATKYDKLNAILTLNIDKLWRKKAVKLCNIK
                     ENDKVLDLCCGTGKMIELECRAVGESTMVVGLDFNKEMINVGYKKLNKSLRGYKFNLIQ
                     GDATELPFEDNSFHCITIAFGLRNVPNKIKAISEMYRVLKPGGKVVCLELSKPKMPVFR
                     NVYNLYFNFVLPVIGYLGTQDKAAYNYLRDSVNDFMTKKQLRHEFENVGFENSGFKSLT
                     CGIASIHYGIKPL"
     gene            13730..14539
                     /locus_tag="LL065_RS20960"
                     /old_locus_tag="LL065_20940"
     CDS             13730..14539
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240612.1"
                     /locus_tag="LL065_RS20960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20940"
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="WP_226136640.1"
                     /transl_table=11
                     /translation="MHILVCVKQVPDTTEIKMDPKTNTMDRSSAPTIINPFDAHAVEEA
                     VKIKKEFGGKVSIISMGPPKAEEVIKKCIEMGADEGYLLTDRAFAGSDTLSTSYILAMG
                     IKKVIEIERIDLLLCGKQAIDGDTAQVGPGIACRLGMPQLTYVESIENFNLKKNVVVVH
                     RKIDNGYEVVQSKLPCLITVEKDINELSFSPLPNMIKAARYKPITWAINDLGGDLSKLG
                     LTGSPTSVQRVFSPKQRVGGELLKGSEDEVVKTLVDKLKGGIRKWQL"
     gene            14527..15585
                     /locus_tag="LL065_RS20965"
                     /old_locus_tag="LL065_20945"
     CDS             14527..15585
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240611.1"
                     /locus_tag="LL065_RS20965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20945"
                     /product="electron transfer flavoprotein subunit alpha/FixB
                     family protein"
                     /protein_id="WP_226136639.1"
                     /transl_table=11
                     /translation="MAAIDKEYSGVWVFVEQLEGKLATVTLELIGKAKKLSSSLNLQVS
                     AVLLGDKVESIMSTLFEHGADTVYIIDDPVFHFYRAETYMRAFCYLVEKYKPEILLMGA
                     TTTGRDLAGAVATTLKTGLTADCTALDIDLEKRILLASRPAFGGNIMATIVCKNHRPQM
                     ATVHPKVMKMPTPEPNRTGIVIKETFTIDEESLKTIVLKIVKDGSENVKLEDAKIIVSG
                     GRGIQNEQVFKLLNELASVLGGVVSGSRGAVEKDLIDHNRQVGQTGKTVSPKLYFAIGI
                     SGAVQHTVGLQGSEIIVAINTDAECPMMKMATYSIVGDALTILPKLIKVFKESLSDLKN
                     LNSNMKGGVSVE"
     gene            15578..16873
                     /locus_tag="LL065_RS20970"
                     /old_locus_tag="LL065_20950"
     CDS             15578..16873
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1119: halogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809581.1"
                     /locus_tag="LL065_RS20970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20950"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_216123720.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.2e-07, bitscore:
                     28.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 1.6e-08, bitscore: 32.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEKFDAIIVGAGVAGLAAAYTMSKAGLKIIVIEKGQHPGSKNVM
                     GGVLYSHMMAEIIPDFWKDAPLERPIIEQNLWIMGKESVVKTGYKDMGWSKAPYNNFTV
                     FRGKFDQWFAKKCVEVGTLIVNETVVKECILENNKVVGVRTDRPDGDILADVIVLADGV
                     NSLLGKSLGFHKEWRKDQVALCVMEELKMPSDKIEERFNLDKGMGATIEIFGDTTLGMV
                     GTAFIYTNKDHISIGCGALLSQMYEKRVKPYELLEYIKNHPMVKPLIAGSQPCEYYAHL
                     IPEGGYKSIPKLMDDGVIVIGDAAQFVNGIHREGSNMGMTSGRFAGETIIRAKGIGDFT
                     KSTLSHYEDLIKESYITKDLKKYKNASSTMENNGAYFNHYMPALNKSVSEMLTVDGASK
                     SKKQKLIMKHMTNGRSMFTAALDLFKMLKEVK"
     gene            16873..17166
                     /locus_tag="LL065_RS20975"
                     /old_locus_tag="LL065_20955"
     CDS             16873..17166
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101891.1"
                     /locus_tag="LL065_RS20975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20955"
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="WP_226136638.1"
                     /transl_table=11
                     /translation="MSDIKKKVNIDDKLFLNTYIADTVSHLIIKDEGVCANCDEKNCTK
                     FCPAGVYQYKDSKIVVGFEGCLECGACRIACPHGNIDWSYPRGGFGIQFRLA"
     gene            17329..17847
                     /locus_tag="LL065_RS20980"
                     /old_locus_tag="LL065_20960"
     CDS             17329..17847
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171712.1"
                     /locus_tag="LL065_RS20980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20960"
                     /product="DoxX family membrane protein"
                     /protein_id="WP_226136637.1"
                     /transl_table=11
                     /translation="MKLLKNKYFNIVWTALRIWLGYQWISSGWGKITGDGWVGSTAGTG
                     ITGFLNSSLIKASGAHPAVQSWYADFIKNIALPNAVTFSYLVAFGELLVGISLILGALT
                     VVGLIAGALMNLNYMLAGTTSTNPNMYTVAFILLFVGANAYTIGLDHFILPILKNFFTK
                     KSDFNQISS"
     gene            17902..18474
                     /locus_tag="LL065_RS20985"
                     /old_locus_tag="LL065_20965"
     CDS             17902..18474
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS20985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_20965"
                     /product="FixH family protein"
                     /protein_id="WP_226136636.1"
                     /transl_table=11
                     /translation="MKLKSLKNFVFTLILVLSVSRSTFASTTDTQINKTVDGVKATLTF
                     STQKVKLGNNDFTIFILDKNGNPLPNLKVTAYADDPNGTDGMTNTSGASGMAGMPGMSG
                     MSASDMPMIALKDGSTKGEYTGMVNFKSTGKWIVESTFDVKGQSKTIDFNVNAQGPNFL
                     IIGVGFLGVIVLIIAIAALNKKKSIKS"
     gene            complement(18615..19502)
                     /locus_tag="LL065_RS20990"
                     /old_locus_tag="LL065_20970"
     CDS             complement(18615..19502)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ubiA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209193.1"
                     /locus_tag="LL065_RS20990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20970"
                     /product="prenyltransferase"
                     /protein_id="WP_226136635.1"
                     /sec_met_domain="ubiA (E-value: 6.4e-17, bitscore: 59.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRFKSIIKLMDIKTLVAGIVPVILGSIYSKYAFLKFNILYFILLI
                     VAMILIQSATNMVNDYSDFKSGADNKKSGDEKALVSGEITSKQVLVVIFLFELTACMIG
                     IFIASQTSYYIMLIAIIGGIISISYAFGPFPISYTPFGEIVAGITMGIGITTTVIYIQS
                     GVINLNTVLVAIPTVIYISTILLSNNVSDLQEDLDVGRKTLPILMGVKNAEKLWIFNVI
                     MLLILTFILCVVHIYPIVVLVTVLIFFPYKSIFHFISYDKNMNTKGRTMELISKIGLKY
                     HLAVVVGLLIAIIF"
     gene            complement(19506..19904)
                     /locus_tag="LL065_RS20995"
                     /old_locus_tag="LL065_20975"
     CDS             complement(19506..19904)
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1144: thioesterase superfamily protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615201.1"
                     /locus_tag="LL065_RS20995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_20975"
                     /product="PaaI family thioesterase"
                     /protein_id="WP_216123710.1"
                     /transl_table=11
                     /translation="MNLIDGLNIEYIHVLDRELEAKMNLTQFHDQTFGYLHGGATIAFG
                     ETITGYASLQKINKDQVAVGQTITANHMKAKKIEGYIIAKGKLIHVGKTSHVWSVEMFD
                     ENNVLISFMTVTNAIIKSNKDNPFGKER"
ORIGIN
        1 tatccttgtt gcaaaacaat aaaaattatc ctatatggct tatctaatat taaagcaata
       61 gatattataa accaaataat ccccatattc cccataaatg taactatagg cattataata
      121 tctaaatatc tattttgtcc atacttttta ataaaaaaat agaatataat catcaatttt
      181 tcgtatttca ttgccaattc tgagatacac cttaccgcct ctttctccta tatactatcc
      241 tatgtattat tattcactat aaccttagaa tgctattccc ctgcatgcga gacatagcat
      301 aatacttatt actaatgtta ttaaatctca gtgccctgaa cctcatcttc attgtcatca
      361 tttttttttc tcttttcttt tataaacact ataactccag gcaataatga aattattatt
      421 atagctacta acatataaga aaaatgagtt tttacaaatg atagattacc aaagaaatat
      481 cctccaccta aaaataaact tacccaaagt cctcctccaa caatattata tggtacaaac
      541 ttcgaataat tcattttgcc aatgccagct acaaaaggtg cgaaagttct aattatagga
      601 ataaatcttg caattactat tgtcattgag ccatgttttt cataaaattc ctgagccctt
      661 attaaatact ccttatttat aaaccttact ttttcttttg cgagtatctt tttcccaatc
      721 tttttaccga taaaataatt agcagtatct ccaagtatag ccgcaagata tgatattatt
      781 aggagggtaa atatattcat tgatccaatt cctgctagtg ctcctgccgc gaataacatt
      841 gaatcacctg gcaaaaatgg tgtaattact aaacccgttt caataaatat aattaaaaaa
      901 agaactccgt atgttaacat accatactgt tgaaccatta aagttaaata cttatcaata
      961 tgcattatta cgtttataaa gttgattaaa atatccatat tttctctcct cgtttttaaa
     1021 cacttatgta gatgtcttga tgctagtatt atatatcggt aagattaaaa tttgattaaa
     1081 aaacatcaca ctttaagata atatcataaa taaaatactt ctggccctcc catactatta
     1141 ctttaagaag ttgctatttt taaattttat tgtaggaagt atacgataaa cttctaataa
     1201 aataatattt atagtaagcc aaactccacc aaaaatgtac cctgcataaa catcacttgg
     1261 gtattccatt ccaaagaata tcggatttaa acctgaaatt gcgcaaataa acaaagtcat
     1321 aataaatact acagttttag accaaacttt ctttgcatga cgtactatta ggtatacaaa
     1381 aaatccataa acaacaattg ccattaaagc ttgataactt ggaaaagtat attgattatt
     1441 acttattaat cttattgaag aaggtcctaa acgcctaaaa atatgcctta aaacatattg
     1501 cagtatttct ccaccaaaaa tcacaataaa tagggactgg atttcaagaa ttcgatttat
     1561 attccttctt ataatccata taaacatgaa gatagtaaga ggtactaata ccctaattga
     1621 tgttgttagt ttaaacagcc ctatcacaaa tgaccattta gaattaaaaa atacttttac
     1681 taagtatgat acaacaatat caaattgatc aaattcatgt gataaataat cctgtaccac
     1741 cccaattatg agaactgaaa agcctaaaaa ggcaactgct acagccccta tagcaacttt
     1801 catttttccc atagaatgaa ctataattat agttttattt aaaactttat atgtattctc
     1861 tattatctgt agtttatgat tcctataagc atatataata attaaaatca aagatataat
     1921 tatactacca ataatcaaat attttgatat gtacccatga aacttatgcc agtttggacc
     1981 aagaacctta cctaatgaaa taaaagtagt agtccacaaa aacgctccaa aataggcatt
     2041 actagcaaac cttacatatg atattttcgt tatacctgaa aaatatcctg taatatgtct
     2101 aacaccaggt atgaaatacg ccaaaattaa taatttattt ccagatgtat taaaccactt
     2161 ggatgttttt tcaattttag cgggaccaag gtgaatatag gaaccatatt tttcaaaaaa
     2221 atttactcct aatttagtac caacaaagta agatatagta atccctagca ttaccccagc
     2281 tgtagctact agaatgctta taccccaatt cattttagat tgataaacga gaaagccaca
     2341 atatgtcatc attaattcac caggaagagg aaatgcaatt aattcaagca ctaaagcact
     2401 gaacaaaatg atatacccat aattattaat taataatatt acagaatgca aaatctcgcc
     2461 tccctatttt aaagattaca aacttgttat atactagcct tggaattatt ctttatatta
     2521 tcaaatatat aaattactaa agcactacat acaagcccta aaattgcacc tactaaaaca
     2581 tctgtaggat aatgtacata taaatatacc ctagaaaaag ctattaaaga tgctaaactt
     2641 ataatttcaa taacatactt tttaaaatat tttgcaagaa cccctgcaac tgcaaatgac
     2701 gacatagaat gccctgatgg aaatgaataa gataatggtc ttgttattaa aaggttcatt
     2761 gttggtatag tgtccgatgg ccgcaaacgt ttaaatatat ttttcagtat ttcatcacct
     2821 aaaattgcgc ttagaattaa agctgctaag gccataaacc ctatttttct atattttttg
     2881 ttacttataa gtatcgatgc tattataatc catattactc ctgcatttcc taagtaactt
     2941 gatataacca taactttatc cataataatt ccatgcatat tatattttat aaacaatagt
     3001 atagagatat cgaatttctg taataggtac atcattatca tctttcctcc tttgattgct
     3061 aggagattct gtaatgtccc ccatgtctta tataatatat tttcaagatt aaaatcacat
     3121 taaattatct ccttcgcagc tgcaatatta gatttaactt aataattaat atttaacata
     3181 acagttatct ttgttccaaa accttccgtg ctttctgcac ttatagttcc tttatgtaca
     3241 tccacaatta atttagcaat agatagtcca agaccagtac cacctttttc tttagatcta
     3301 gatttatcaa cagtataaaa tctttcaaat acattttgta tttcatcttc tggtattccc
     3361 atgccactat cactaacagt aatttttact gtattacaat aaatctctga acttatatct
     3421 attgtacctt tttctggggt gaatttaata ctattatcaa ttaatatcct aagcatctgc
     3481 ttcatcattt tataatctgc aaatatacta atggaatcat ttttgtaact tgatattata
     3541 tgactatgat ctataagttt agtttcacta atcacttcgc aaataagatc attaagcaag
     3601 aaatctcctt tctcaattct ttgatttccg ctgctccctt tagcgataaa caataatttc
     3661 tcaactaaat ttgccatatt actagtttct agttttatag cgtgaataga tttttccaag
     3721 gcttctctgt catctttacc ccatctatcg attaggttcg catacccttg gataactgca
     3781 atcggagttc taagttcatg tgatgcatca gacacaaact gaatttgcct atcaaaagat
     3841 tcttgaagcc tatctatcat tttattaaaa gttataccca atcttttaag ttcatcatca
     3901 ggacctgtaa cctctattct tcccaataaa ttattaatac taatattatc agcagcttta
     3961 gttatttgat caataggttc taacatcttt ttacttatga tgtaccctaa tataattgaa
     4021 gcaatcatcc ctataaaatc agctatcgcc attaaaataa atagaatttg cataaagtca
     4081 tattcattac acatccactt aactatttga atataaatga ctccatattt ttgggtttca
     4141 atctttaaaa ttttatatac taggtgttta tcatcttctt taatttttct tattttatca
     4201 aaaggttctt tgcttataat attataatta aattcttttg atttatttag tattttgcca
     4261 tcttgcttta gtattcttat tgatatattt tgttctgaag taacatctga aataagttct
     4321 ttatctgaca aatctccatg ttcattccga gatacgatat tatttaatac tattgttttg
     4381 acatcttcta tttgtttatt tgcctgaccg tacaagtagt actttatacc atatagaata
     4441 gaagcattta aaataagtaa tattaatgag aacattacag cataaatcac cgttaatttc
     4501 aaagatattt ttgcactttt gatcattttc aaattcataa ttttaggacc cataatttta
     4561 aaattcatgg ttactcacct ttaacaacat atcctacccc tcttacagtg gttataagtt
     4621 tatcctcaaa gccatcatca attttacatc gtaaatatct tataaataca tctacaacat
     4681 tagtatctcc tatataatca taaccccata ccttctcaat taattgtact cttgtaagta
     4741 caacgttttt gtttattaaa agcatttcta aaaggtcaaa ctcttttttt gtaagttcaa
     4801 tttctttgct gcctctctta acattatgcg ctttattgtc cataactata tctttcactt
     4861 taatctcatc gctcttatta cctatagatg tatctctttg atacacccta attcttgcta
     4921 atagttcttc tattgcaaat ggttttgtga gataatcatt tgcacctaca tctaatccta
     4981 aaaccttatc tgatatatca ctttttgcag taagcataat tacaggtacg agcgacaact
     5041 gccttattct tctgcaaacc tcaattccat taagacccgg caacataaca tcaagaagta
     5101 ttaaattata ctcattttcc tcagctattg ttaaaccttc tcgtccatca taagcaacat
     5161 caactttata tccttcatga ctcaattcca tttcaataaa tgttgacatt agcctttcat
     5221 cttccactaa aagaattcta aaatccttca aaatagcacc ttccttttac gataatctag
     5281 tgagaaaaca aatcccatat actactaata tacactatct tcgtagtctt agccatgttt
     5341 atgagtattt catgtatgct cattcttatt aatactatta tattaataaa ataaataaca
     5401 tgtaaaaaat acatatgatt tctcatatgt atttttacat gaaatataaa ctattattat
     5461 tcttttgaaa ctatcacttt tctgatatat taattatcat ttgtcctttt tttgaagtag
     5521 cgtcaacaga aatattactt tcttctgatt tgttactctt tttttccttt tgtgcaggat
     5581 ccccattaga acaatcgcct gaatctgctt tactactcat ctgtccttct ttggaattat
     5641 cattatctga ttgatcactc ttttgtttct tttccgaagt atcatcatct gaagagtttg
     5701 aacctacatt agcgcctgaa atattaccgt taaaagggat tgtaatttca atagtatttc
     5761 tgtttttttc tggtttttct aaatcttttt cacccataaa tattctccct tcatatataa
     5821 aactacacta tatacatagc gtgcattata tagtttaccc atttaattat aaaatatagt
     5881 ttatatcgaa catattgcat tgacttgaag agcttaatgt attataaata agataattat
     5941 aatactaagc ctgatgagat taatagttat tatggcatta gtaattatta caactacatt
     6001 atacaaggag tgtataaact tgaataatat attgattatt gaagacgagg aaaagatttc
     6061 tgaaatacta aaagcatatc ttgaaaaaga aggctatagt gtgtatacca ctacaaaagg
     6121 tttagacgca ataagacttt tcgagaagat tcaatttaat ttagttatat tagacctgat
     6181 gttaccagac atatctggag aagaggtctg cagcatttta agaaaaattt ccgaggtgca
     6241 catatttatg cttactgcca aaagcacctt agaagatagg atagaaggct taaaccttgg
     6301 ggcagatgaa tacttgatta agccgtttag tccaagagaa ttaaccgcaa gagtaaatgc
     6361 tttatttaga aggattaata taaataaatc tgaaacaatg gtttatgaca atggagattt
     6421 agaaattaat tatgacaaaa gagtagtttt tgtgcgaggg gaagaaatat cacttactcc
     6481 aaatgagttc gacatattac agattctttc atctaacggt ggcaaagtat ttacaagaga
     6541 acatctcatt gaaagaatta tgggaattga ttttcaaggt tttgatagaa ccatagatgt
     6601 acacattaaa aatatacgta aaaagataga attggatagc agaaatccta aatatataat
     6661 aacagtaact agaatcggtt ataaatttgg tggtgattaa tttatgcaaa gcatacgaaa
     6721 aagattaagt attatattag tatgtacatc catattagca ctctttttag ctacactttt
     6781 tgtaaatata gcaatatcaa aaacctttaa caagtattta gttgaaatac aaactaagaa
     6841 ggacactgga atagtaaaat atttagaaac agtgtatgaa aagaatggta agtggtcaaa
     6901 aacttctgga atagaaatta cgcatgaagc ctatatgagt aattactgcc tcactcttaa
     6961 ggatataaat aaaaaagttg tttggggaat gaatgcaaat gacataatta acaacactaa
     7021 tactactatg ccccataaaa gtaacggcgt atatacttta aggaccctcg aaataaaata
     7081 taaagataaa atcatagggt atgtagacat aggacaaaat ggtccagtat tattaaccag
     7141 tgatgatatc acttttaaga catcaataaa caagggcatt attttaagta ttgtactctc
     7201 acttttgata acaatattaa ttagtttgct cgtttcaaag caattttcta accctcttag
     7261 gcaagtttct aaaatttcag ttgatctctc acatggtaac taccgtgcac gctcatatga
     7321 taaaagcacc attttagagt ttgaaaattt aagaaatagt ataaatacac ttggtgaaaa
     7381 ccttcaaaat caagatgcta ttagaaaaag attaatatca gatatatctc atgaagtaag
     7441 aacccccctt aatattcttc aaaacaactt tgaagctatg attgatggta tttttccggc
     7501 agacaatgaa agattaacct atcttaacga tgaggttatt agattcggaa agcttataga
     7561 caatttaaat accttgaaac agattgaagg agaagaaacc gccttaaata tggaaataat
     7621 ctctttaaaa gatatcctca ctttagtcaa aaaagagttt gagctattat cagaaaataa
     7681 gaatatcaca atgacattaa aatgtagcac caataaccaa tgtgatattt tgggagacac
     7741 agataaaata aaacaagtca ttattaattt actttcaaac gcggttaaat ttacccaaaa
     7801 tcaaggagaa attaatatta ctttaaagga atctaataaa aaaataatgt tgaaaattca
     7861 agatacaggc attggcatta aggaagagga cttaccttat atatttgaaa gactctatcg
     7921 aggagataaa agtagacatg aaatagaagg ctctggtata ggccttacaa ttgtaaggaa
     7981 tatattgacc ctccacaagg cagacataga agtaaaaagt acagaaggaa aaggaagcac
     8041 gtttatttta tattttaata aagttttgtg attattatcc aaatattcaa ggaatcttaa
     8101 acagcaaagt cacttgaatt atatccataa gtaagtttga agagtagagc actctcttca
     8161 tattttcttc acaattacag tttataattt agttatatta agtaaataaa tctaaactta
     8221 ttcaaggagg ttatttatat gtattgtaat agatttttag gatctggata tggcactgga
     8281 gtggggatgt ttctaatgat ggtctttggg cttttgatat tttcagcatt tgtttttcta
     8341 gttttcaaac taatgaaagt taattcgcct tcatctttct catcaagtaa tttggcttta
     8401 gatatactaa atgaaagata cgctaaaggg gaaattaatg aagaagaata tactaaaaag
     8461 aacacaattc tttctagaaa gaactaggaa tagtttcact cttacgcttt ctaaataact
     8521 attaaatcca gatagataac ctatctggaa atactgcttt tagaattacc ttagcatcaa
     8581 taattccttt tgtttgtcca ctaatgatga ttttactgat accgatgatg ataaaatgtt
     8641 tagaagaagg taaatcatgt catgataaat tcatgattat atctattacg tattttttat
     8701 tttactcttc tagctgttac ataatcattt cttcccaaag ctgaaatctt aattacatct
     8761 cctgtatgag gagcgtgtat ataattatta tctccaatgt acatacccat atgatgagga
     8821 tttgcaaagg ttccaaagaa caccaagtcc ccaggctgaa gattatccct tgatacttct
     8881 acaccatcat ttatctgatc aaaggttgtt cttcctacag atattccaaa atgtgcgtat
     8941 acatattgcg taaatcctga acaatcaaag cctgatggcg ttgttccacc ccataaatat
     9001 ggtgtgccaa gaaaattaga agcataagct attatagcac cttgtgatac tgttgctcca
     9061 ccccttgaag gtgtatatgt agacactgat ttttttaatt cgcttatttt cgttaaactt
     9121 ttatttgctg acacttgagg ttcaattatc tgcgctgaaa tattcttatt acttttactt
     9181 tcagtaatca atttattctg taattcttta gcaacaattt ccttgttcct taattcagtt
     9241 attaatttgt tttgtgacgc tttagtaacc attattttat caatcttctg tttattttct
     9301 actattaaac aatccctgtt ctttctgcga ttcctttgca acctttttaa catccactgc
     9361 ggcaacttca attgactttt cagtttcaga tattttccct ttattctctt ctgttttagt
     9421 catgattttt caatttcatt atcaaactct tctattttca tttcgacttg ttctttctcg
     9481 gtcttaatta tagttagtga atctttctgc tgtttagttc tgcaactttt ctcttgactt
     9541 ctatacctat taagtaacta acaatttcaa gttacttctt cttttgcttt gaggaacaat
     9601 ttccacatcc acaatctcct gaaccttttg actttagact atgataaata atatgtcctg
     9661 ctatagctat aataaacaat gctattgata cttcaatcca cataaacttc atcctcccgc
     9721 aattaacatt aataaatgtc ataatatcta ttaaaccttt tttatagtga ctaatatctt
     9781 tttttactat atcaatttca tgtgtagatt ttataaagaa agccttcgat tttcacgtta
     9841 gggtcgctac ctaaggtata tctaatttat ttttattaga tataccttct acataatatc
     9901 ttcataagat tttaatataa tcagtattgt acttgcaaaa cattttcaac agtaagtact
     9961 taaattaaat aattacttga ttccgcaata tagaaaggtg gtggaaaatt tgcagtttaa
    10021 agatatttat tgcccaataa gtaaagattt aaatctcgta gaaaaagaac ttagaaaaac
    10081 atgcgaaaat ttaagcacta attatttaca agaaattata ggctatttct ttaaaattcc
    10141 aggtaaaaga cttcgcccta ctttagcttt gctctcatcc ggcattataa acaataatct
    10201 tccagagagt accaataatc aattaattaa gtatgcaact ggccttgagc tcatgcacag
    10261 tgctagttta atacatgatg atgtacttga taacgatatg gttcggcgtg gtcaaaaaac
    10321 tctaaatgaa atttatggca ataaaatagc agtactcgca ggcgatgttg tttattcttt
    10381 agcattctct actctctcga gttcactacc aaaagaattt aaacaaacta tagttgaatt
    10441 aaccgaaaat atgtgtgctt ctgaagtgat tcaagcagaa aatatgttac ctactcgtga
    10501 aatttattta gatataatta gaggcaaaac agcattattt atgtctgtta gttgcaaaat
    10561 tgcagcagct ctagctggag caacaaaaga acaagttaat agcatagaag agtatggttt
    10621 aaatttagga atgacatacc agataatgga tgattgtatg gataaagatg tttatgctga
    10681 gctaaatata acgtctgagg atgctaaatt atatggaaat atagcaattt catcacttaa
    10741 gacctttgag gattctgcat acaaaataag tttgataaat cttgtgaatt tcattcttag
    10801 tctttcacat acaaaagtaa ataactctta atgaaaatgt taaaattttc aaatcaatga
    10861 ctaaatacaa ttcattataa aatttaattt caataactaa attctatatt caataattaa
    10921 tacaatataa aaaatattct ccaaaacgaa aggggcccta taaccatgaa aattaaatta
    10981 tttaaaaacc ttgtatttac cttaatattg gctttatcat tttcaacatt agcatttgca
    11041 tctacaactg gtactcaagt aaataaaact gttgatggtg taaaagcttc attaaccttt
    11101 gcaactgaaa aattgaaacc aggaagtaac gaatttacaa tctccatact tgataaaaat
    11161 gggaaacctt tacctaatgc taatttaaaa gttactacaa atatggataa ctctactaat
    11221 atgactggta tgagcggaat gtcaacagat aataaaccca tgatgatagt tctaaaacag
    11281 cgttctatga agggtgaata tacaggcatg gtagacttta caagtacggg taaatggatt
    11341 gttaaatcta cttttaatgt acaaggacag gcaaaaaaca tttattttaa tgttaatgtt
    11401 caaaaatcag gccctaactt tcttataatt ggcttcgcag gagtaatggt actaattata
    11461 gttgtagctg ctataaataa gaaaaaatct attaaatctt aaaaatggag gcctcataat
    11521 aatggcaaat ataaaaacac ccttaaaacc cgatttatcc acaacaactg aaaaaaaaca
    11581 taaggatttc ctaaaaaata aaactttcaa aaaattcatt aaaagtaaat ggtatcctgg
    11641 tatattccag tggccaatag ctctagtttt cgcctttatc gtctatgaat taatcgctgg
    11701 accaaccgac gctcatgata actttggtac tgctggaact tgggtattat ggtggccgct
    11761 actcccaatc cttctactac ttttcggtag attttggtgt attttatgtc cttttgggct
    11821 tctaaatgat gtggttcaaa aatttgtagg caataataaa cccgtaccaa aatttttacg
    11881 aaaatatgga atttggatta ttgatgcaac ttttcttatg attacctgga gtgatcatgt
    11941 ttggggcgta gttgagaacc ctcgaggttc aggagtatta cttttaatga taactactgc
    12001 agtaatattc tctggtgcac tctttgagag aaggacttgg tgcagatatt tatgtttcct
    12061 tggtggattg tctggtaact attcgagagc tggtatggtt gaactacgtg ctacaccaga
    12121 agtatgtaaa aattgtaaaa ctgcctcttg ttacaaagga acaactgaag ttcctggatg
    12181 tcctatgttc gaattcccta aaaccatgga aagtagtgca gaatgtaact tatgtggtaa
    12241 ttgcgttaaa aattgtccaa atgactcgat tagaataagt ccacgtgttc ccactgaaga
    12301 attatgggat attaaaaaac ctagagtaga agttgcattt ttggccattg taattatggg
    12361 tattgtattt gttcaaaaca taactatgct aacgatatgg gaaaagatat tacattctct
    12421 tgaaaatata ctaggaacaa caaattatta tataacattt actgtaactt tcattatagc
    12481 tatgctaatt cctgtcgtgt tactgctttt aacaggtctt attgctaaaa aattcaacaa
    12541 ggcttccgta atggaaaact tcgctaaatt tggttatgct attatccctt tagacttagc
    12601 aggacatata gctcataatc tatttcactt gctagcagaa ggtaaatcag tgatttatac
    12661 tataatggaa cttttcggag tgtcatctca tggagcttca actgcaatac tccctagtcc
    12721 gtctattcaa attcttcagt atatcattat tgtaattggt actgtaggtt caatatacac
    12781 tgcttataga attgctaaga gcagtcaaac taaaacaaca cttttaggaa cctcagtagt
    12841 ttatgcaatt ctaatggtat tacttgcaat agcaaatata atattatttt ctattccaat
    12901 ggcaatgcgt atgtaacaaa ttaaatagta aagggaggcc tagataatgt cagaaacgat
    12961 caccgatgta caaggtatat tctcatcaat tgctactaaa tatgacaaat tgaatgcaat
    13021 attaacttta aatatagata agctttggag aaaaaaagcg gtaaaactat gtaacattaa
    13081 agagaatgat aaagtattgg atttatgttg cggtacaggt aaaatgatag aacttgaatg
    13141 tagagcagta ggagaatcca ctatggttgt tggtctcgac ttcaataaag aaatgattaa
    13201 tgtaggatat aaaaagctta acaaatcatt aagaggttat aaattcaact taatacaagg
    13261 agatgctacg gagcttccct ttgaagataa tagctttcat tgtattacta tagcatttgg
    13321 tttacgaaat gtacctaaca aaattaaggc tatttctgaa atgtataggg ttttaaaacc
    13381 tggtggaaaa gtagtttgct tagaattatc taagcctaaa atgccagtct ttagaaatgt
    13441 atataattta tattttaact ttgtactacc tgttatcggc tacttaggta ctcaagataa
    13501 agcggcttat aactatctaa gagattcagt aaatgacttt atgacaaaaa agcaactacg
    13561 acacgaattc gaaaatgttg gctttgaaaa ttcagggttt aaatccctaa cttgtggaat
    13621 agcttctata cattatggca ttaaaccctt ataaaaatga ttatgcacct ttagtttacc
    13681 taaatctaaa attaaaaaag agctgttatt taaaaggagt tgttattaat tgcatatctt
    13741 agtttgtgta aaacaagtac ctgatactac agaaattaaa atggatccca aaacaaatac
    13801 catggataga tcaagtgcac ctactattat taatcctttt gatgctcatg ctgttgaaga
    13861 agcagttaaa ataaaaaaag aatttggggg gaaagtttct attatctcca tgggacctcc
    13921 aaaggcagag gaagttatta aaaaatgtat tgagatgggt gctgatgaag gatatctgct
    13981 aacagaccgt gcctttgcag gatcggatac actatctact agttatatcc ttgctatggg
    14041 aataaagaaa gtaatagaaa ttgaacggat tgatttgctg ctttgtggta aacaagccat
    14101 agatggagat accgcgcaag taggaccagg tattgcctgt cgtttaggta tgcctcaatt
    14161 aacttacgtt gaatctattg aaaatttcaa tttgaaaaag aatgttgttg ttgttcatag
    14221 aaaaattgat aatggctatg aagttgtaca atcaaaactc ccatgcctaa taacagtaga
    14281 aaaagatatt aacgaattaa gcttttcacc tttaccaaat atgattaaag cagcccgtta
    14341 taagcctatt acttgggcaa ttaatgattt aggcggcgat ttaagtaaat tggggttaac
    14401 tggatctcca acttctgtgc agcgcgtctt ttcacctaag caaagagtcg gtggtgaatt
    14461 gcttaaaggt agcgaggatg aagtagttaa aactttagtt gataaattaa aaggaggaat
    14521 acgtaagtgg cagctataga taaagaatat tccggtgtat gggtatttgt agaacagtta
    14581 gaaggaaaac tagccaccgt taccctcgaa ctcattggta aagcaaaaaa actttcttct
    14641 agtttaaatt tacaagtatc cgcagttctt ttgggtgaca aagtggaatc tattatgtct
    14701 acacttttcg aacatggtgc agatacagta tatattatcg atgatccagt atttcatttt
    14761 tacagagctg aaacctatat gagagcattt tgctatcttg tagaaaagta taaaccagag
    14821 atattattaa tgggagcaac aaccactgga cgcgatcttg ctggcgcagt tgcaactaca
    14881 ctaaagacag gattaactgc tgattgcacc gctcttgata ttgatttaga gaaaagaata
    14941 ttacttgcta gtaggcctgc ctttggtgga aatattatgg ctaccattgt atgtaaaaac
    15001 catagacctc aaatggccac tgtccatcca aaggttatga aaatgcctac ccctgaacca
    15061 aataggactg gcatagtgat aaaagagaca tttaccattg acgaagagtc tttgaaaacc
    15121 atagttctaa aaatagtcaa agatggatca gaaaatgtca aacttgaaga cgcaaaaatt
    15181 atagtgagcg gcggtcgtgg tatacaaaac gaacaggtat ttaagctact taatgaactt
    15241 gctagtgtcc ttggaggtgt tgtttctgga agccgcggtg ctgttgaaaa agatttaatt
    15301 gatcataacc gtcaagttgg tcagacaggc aaaacagtat ctccaaaact atatttcgca
    15361 ataggaattt caggagccgt gcaacatact gttggccttc aagggtctga aatcatagtt
    15421 gctattaata cagatgctga atgtcctatg atgaaaatgg ctacttatag cattgttgga
    15481 gatgcactta caatattgcc taaattaatt aaagtattta aagagtctct atctgatcta
    15541 aagaatttaa attccaatat gaagggaggg gtaagcgttg agtgaaaaat ttgatgctat
    15601 tattgttgga gctggtgtag ccgggcttgc agcagcttat acgatgtcaa aagcaggcct
    15661 taaaattatt gtaattgaaa aaggtcaaca tcctggttct aaaaatgtta tgggtggcgt
    15721 cctatacagt catatgatgg cagaaatcat ccctgatttc tggaaggatg ccccactcga
    15781 gcgccccatc attgaacaaa atttatggat tatgggtaaa gaatctgtcg ttaaaacagg
    15841 ctataaagat atgggatggt caaaagctcc ctacaataac tttactgtct ttagaggtaa
    15901 atttgatcaa tggtttgcaa aaaaatgtgt tgaagttggt acactgattg ttaatgaaac
    15961 tgtagtaaaa gaatgcatac tagaaaataa taaagtggtt ggtgttcgca cggacagacc
    16021 tgatggagat atattggctg atgtaattgt tcttgcagac ggggtaaatt cgctcctagg
    16081 taaaagtctt ggctttcata aagaatggcg caaagaccaa gttgctcttt gtgttatgga
    16141 ggaattaaaa atgccttctg ataaaattga agaacgcttt aaccttgata aaggaatggg
    16201 agcaaccatt gaaatatttg gtgatactac tttaggcatg gtaggtaccg ccttcatcta
    16261 tacaaataaa gatcatatat ctattggatg cggtgcatta ctctcacaaa tgtatgagaa
    16321 acgcgttaag ccttatgagc tattagaata tataaaaaat caccctatgg ttaagccctt
    16381 aattgcggga agccagcctt gtgaatatta tgcacactta attccagaag gtggttataa
    16441 aagcattccg aaattaatgg atgatggagt tatagttatc ggtgatgcag ctcagtttgt
    16501 taacggtatt cacagggaag gctcaaacat gggaatgact tcaggacgtt ttgctggtga
    16561 aacaattata agagctaaag gtattggaga cttcacgaaa agcacccttt ctcattatga
    16621 ggatctaatt aaggaaagtt acattacaaa ggatcttaag aaatataaaa atgcttcctc
    16681 tactatggag aataatggtg cttacttcaa tcattatatg ccagcactca ataaatctgt
    16741 aagtgaaatg ttaactgtag acggtgcaag taaatctaaa aaacaaaagt taataatgaa
    16801 acacatgact aatggcagat cgatgtttac tgccgcttta gatttattta aaatgttaaa
    16861 ggaagtgaaa taatgagtga tataaaaaag aaagttaata ttgacgataa attgtttttg
    16921 aatacttata tcgctgacac agtatcacat ctaattatca aagatgaagg tgtttgtgct
    16981 aattgcgacg aaaagaattg tacaaagttt tgtcccgcag gagtatacca atataaagat
    17041 tctaaaatag ttgtagggtt tgagggatgt cttgaatgtg gtgcctgtcg aattgcatgt
    17101 cctcacggta atattgattg gagctatccc cgtggaggat ttggaataca gtttagatta
    17161 gcataataat aaaacctttc tacatattat cttcacaact ttttaatatt atagtacagt
    17221 gcatacaaat cttattctta gtctttccca tgctaagtaa gtctctcatt ataaaaatgt
    17281 taaactttct aaacaaatat acaaataata atttaaggag attttaaaat gaaattactt
    17341 aaaaataaat acttcaacat cgtatggaca gcactacgta tatggttagg ttaccagtgg
    17401 atttcttctg gatggggaaa aatcactgga gatggatggg ttggtagtac tgctggcact
    17461 ggtataactg gatttttaaa ctcttcatta attaaagctt ccggtgctca cccagcagtt
    17521 caatcgtggt atgctgactt tatcaaaaac atagcactac ctaatgctgt aacatttagt
    17581 tatctagttg catttggtga gcttcttgtt ggcattagct taatactagg tgcgcttaca
    17641 gttgttggcc ttatcgctgg tgcacttatg aacctaaatt atatgttagc tggcactact
    17701 agtacaaatc caaatatgta tacagtagca ttcatactct tatttgttgg ggctaatgcc
    17761 tatactattg gacttgacca ctttatacta cccattctta aaaatttctt taccaaaaaa
    17821 tcggatttta atcaaatttc ctcctaaaat gaattttttt aaagattaaa ataagtattt
    17881 ttaaaaaggg gcccaataaa catgaaatta aaatcattaa aaaactttgt atttacctta
    17941 atattagttt tatcagtttc aagatctaca tttgcttcta caaccgatac tcaaataaac
    18001 aaaacggttg atggcgtaaa agccacctta accttctcaa ctcaaaaagt gaaactaggt
    18061 aataatgatt ttaccatttt catacttgat aaaaatggaa accctttacc taatttaaaa
    18121 gtcactgcat atgcggatga tcctaatggt acggatggta tgactaatac gagtggtgcg
    18181 agtggtatgg ctggtatgcc tggtatgagt ggtatgagtg caagcgatat gccgatgata
    18241 gctttaaaag acgggtccac aaaaggcgaa tatacaggca tggtaaactt caaaagtaca
    18301 ggtaaatgga ttgttgaatc tacttttgat gtgaaaggac aatcaaaaac cattgatttt
    18361 aatgttaatg cgcaaggccc taactttctt ataattggtg tcggattttt aggagtaatt
    18421 gtactaatta tagctatagc tgctttaaac aagaaaaaat ctattaaatc ttagacatag
    18481 aggcctcata ctaatcgcat gtccacatga taatattgat tggaactacc ccagtggagg
    18541 atttggtgta cagtttaggt tagtaattaa acaatcataa agggtgtgca atttattgaa
    18601 caccctttat atttttaaaa tatgattgct attagtaatc cgacaactac agctaaatga
    18661 tattttaatc caattttact gataagctcc atagttctgc cttttgtatt catgttttta
    18721 tcataagata taaaatgaaa tatagattta taaggaaaaa atattaaaac agtcactagt
    18781 acaacaatcg gataaatgtg tactacacat aaaataaatg ttagtattaa aagcattatt
    18841 acattaaaaa tccaaagctt ttctgcattt tttacaccca tcaaaatagg aagtgttttt
    18901 ctaccaacat caaggtcttc ttgtaaatca cttacgttat tagataataa tatagtactt
    18961 atatatatta ctgttggaat tgcaactaaa actgtattta ggttaattac accagattga
    19021 atataaatca ctgttgttgt tataccaata cccatggtta ttccagcaac aatttcacca
    19081 aaaggagtat atgaaatcgg aaaaggacca aaagcatatg aaattgaaat aatacctcct
    19141 ataattgcaa ttagcataat ataataactt gtctgactag caataaaaat accaatcata
    19201 caagcagtaa gctcaaacaa gaatatgaca accaaaactt gttttgaagt aatctcccca
    19261 cttactaaag ctttttcatc accacttttt ttgttatctg ccccactttt aaagtcagag
    19321 taatcattaa ccatattagt tgcactttgg ataagtatca tggcaactat taataatata
    19381 aaatataata tgttaaattt taaaaaagca tactttgagt aaattgagcc taaaattaca
    19441 ggtacaatcc ctgcaacaag agtttttata tccataagtt taattattga tttaaacctc
    19501 attttctacc tctccttacc aaatggatta tctttatttg attttattat ggcgtttgta
    19561 actgtcataa atgatattaa tacattattt tcatcaaaca tttcaacact ccaaacatga
    19621 gatgtctttc ccacatgtat taactttccc tttgctataa tatatccctc tatttttttt
    19681 gctttcatat gatttgcagt aatagtttgc cctactgcta cttgatcttt atttatcttt
    19741 tgaagtgaag cataacctgt aattgtttct ccaaaggcaa ttgttgcacc tccatgcaaa
    19801 tatccgaagg tttgatcatg gaactgtgtt aaattcattt ttgcttcaag ttccctatct
    19861 agcacatgaa tatattctat atttaatcca tctattaaat tcacactatt caccacctgt
    19921 tttaatatag tctattgtat actctcttaa agtaacttca tcaatttcca cacctaaccc
    19981 atagccttta ggaactttaa ttataccatt ttgagcaata atttctggtt taattatatc
    20041 ttttttaaag taacgctttg atgatgataa atctccaggg atgaaagtat cctttaaact
    20101 agctaattca acatgcaata ttttagaaat accactttca accatgcttc caacccaata
    20161 ttttatattg ctttccctgc ataaatcaat catttgcttt gcataataaa ttccaccaac
    20221 tcgacctatt tttatattta gcaccttaaa tgcatttgcc gcaattgcac tctttaaatc
    20281 cttaattgtt tgaatgcttt catcaaggca taccggcgta ataatttctt tttgaagttt
    20341 ttgatattct aaaaagtctt ttgaattaag tggttcttct atgcaaatta aattaaattc
    20401 atctaatttt tttatttcat ttatttgatc aaccttataa cttctattag catctactaa
    20461 caattgaatg cttgggtaaa tttttctaat agcccttaac ttttcaaaac catcggccgg
    20521 tttaatcttc attttaaagc gagtatatcc ttccttttga taatcgtcga tttgttttaa
    20581 aagcatttta atatccaaat cacctagtac aatccctgca tagatctcat cattagtttc
    20641 ttcattaaat actagtttca taatggataa cttatttctt ctagcatata agtctaccaa
    20701 ggcattttcg agtcctgaca gtgccatggg gcacgagcta tctaaatctt tatgtatatc
    20761 aaaaggatga tccatatctt tacgaattac agctttaata tatttattta ttaatacctt
    20821 ctttgaagat g
//
