LOCUS       NZ_CP086140            20794 bp    DNA     circular CON 20-JAN-2024
DEFINITION  Clostridium estertheticum strain CF016 chromosome, complete genome.
ACCESSION   NZ_CP086140
VERSION     NZ_CP086140.1
KEYWORDS    RefSeq.
SOURCE      Clostridium estertheticum
  ORGANISM  Clostridium estertheticum
            Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5053333)
  AUTHORS   Wambui,J., Stevens,M.J.A. and Stephan,R.
  TITLE     Clostridia strains as spoilage organisms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053333)
  AUTHORS   Wambui,J., Stephan,R. and Stevens,M.J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2021
            Assembly Method                   :: unicycler v. 1
            Assembly Name                     :: CF016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Nanopore/Illumina miniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 05:10:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,249
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 5,019
            CDSs (with protein)               :: 5,019
            Genes (RNA)                       :: 135
            rRNAs                             :: 15, 14, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 13 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 22 of 95
            Pseudo Genes (incomplete)         :: 78 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:27:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3941479
            Orig. end                         :: 3962273
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086140.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20794
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="7"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20794
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20794
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10794](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10794)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    341..361
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 28.85"
                     /tool="antismash"
     misc_feature    complement(1304..1318)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.98"
                     /tool="antismash"
     misc_feature    complement(1305..1320)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 25.04"
                     /tool="antismash"
     gene            complement(1447..3912)
                     /locus_tag="LL065_RS18590"
                     /old_locus_tag="LL065_18565"
     CDS             complement(1447..3912)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025: diguanylate cyclase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /locus_tag="LL065_RS18590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18565"
                     /product="EAL domain-containing protein"
                     /protein_id="WP_226135678.1"
                     /transl_table=11
                     /translation="MAILTILYFLCGITYVILGIITFLNDSKNELNKLFSVMAINLALW
                     SFMFFLMNCSRNAKAASDFYLYSTFFWSIFYCIFLNFIIILTNKGVFFKKKFAYIILYL
                     PAIISIYLYVFHTETSQNFVETNLGWAYIASKNKGFIWSNFFNIYWFSYIISVIFLLFK
                     WWRNSEIVRDRKQAKLILVTTFIAVVAGGITDSVIPMIRRPVMPFIGIIFIVIPVIGVW
                     YSIKKYKLMDLNHENFALEVLKIMSEGLIIANHEGIIKDINKGALKLLGYDKSRIINKP
                     INYLFADTIELSKLTNCSSFEIEVLQSNNNKLSILLSSSVLKDEWGDSLGIVCIFQDIS
                     EIKLVQQKLKKSYDELEIKVGERTRELSSSNEELEREISSRIDMEFKIKKLAYYDFLTG
                     LPNRTLFIDRLNQGIFNADLSKSFLGVLFLDLDSFKRINDTMGHAKGDELLKMVSKRLT
                     NTMLESDTVCRVGGDEFLILIQNIKNNEDIKAVSEKILTDLKKPFIIDNTDLYITTSIG
                     GSIYPIDGSDVETLIKNADIAMYKAKEKGRNKFELCTQIIKESLVEEMRLTKSLFGAIE
                     RNELELFYQPQISLITGKIIGLEALIRWNNTELGMVNPIDFIHIAEKTGLILPIGEWVL
                     KTACKQNKEWKDKGILNVPIAVNISVNQFQNTKIIEDIITILNETGLDPNDLEIEITEN
                     IIMKETEYIIESLKQLKQLGVKIAIDDFGTEYSSLNYIKQLPVDKIKIDISFVRGININ
                     NKDEAIIKVIIALAKNLELKVIAEGVETKEQIEFLKKEGCDEIQGYYYYKPIPAKKIEE
                     LMKKINKCQ"
     gene            complement(4078..4713)
                     /locus_tag="LL065_RS18595"
                     /old_locus_tag="LL065_18570"
     CDS             complement(4078..4713)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF010075.0"
                     /locus_tag="LL065_RS18595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18570"
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="WP_226135679.1"
                     /transl_table=11
                     /translation="MSKILYITANPKTKENSFSLAVGNEFLEAYKKSNPSDEIITLDLY
                     KTEVPFIDEVVFSAWGKLGAGVTFEKLDKEEQNKVVAMNNLLDQFIAADKYVFVTPLWN
                     FTIPPMMKAYLDNICIVNKTFKYTQNGPIGLLTDKKAVHIQARGGVYSVGLAADLELGD
                     RYINTILNFIGITNKQSIIVEGMNSAPDKADEIKINAINEARKVAQKF"
     misc_feature    complement(4896..4909)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 25.85"
                     /tool="antismash"
     gene            4924..5238
                     /locus_tag="LL065_RS18600"
                     /old_locus_tag="LL065_18575"
     CDS             4924..5238
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613849.1"
                     /locus_tag="LL065_RS18600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18575"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071611654.1"
                     /transl_table=11
                     /translation="MDKFHLCPRFEAAFQILGKRWTGLIIRSLLSGSKRFSELQEIIPA
                     LSSRVLTERFKELEELGIVTRSVYPEMPVRIEYGLTEKGKDLEKTMDEIQKWAEKWPDK
                     "
     misc_feature    5383..5398
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 25.84"
                     /tool="antismash"
     misc_feature    5385..5399
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.98"
                     /tool="antismash"
     misc_feature    complement(5386..5401)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.23"
                     /tool="antismash"
     gene            5428..6132
                     /locus_tag="LL065_RS18605"
                     /old_locus_tag="LL065_18580"
     CDS             5428..6132
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392732.1"
                     /locus_tag="LL065_RS18605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18580"
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="WP_226135682.1"
                     /transl_table=11
                     /translation="MIFIYSLIFVLILSLFFTSNIKKNYWTYYLISIFIGIITTVYEVL
                     RITSDVKLYGFPLFLEKIFIRGYISIAFFILVMFAGALNPKWKITRKLQSIRAELAIIA
                     SIFIIPHALIYFVRFIVLKLPKMFSTGVIPKLYIAYIIAGIIAFVIMIPLFITSFKRIR
                     SKMQSGKWRKLQKRAYIFYFLVYVHILIIMLNEKEIDLLKLSSYTIIFGTYTILRLIKY
                     KNNRQSKLKIAS"
     gene            complement(6183..7349)
                     /locus_tag="LL065_RS18610"
                     /old_locus_tag="LL065_18585"
     CDS             complement(6183..7349)
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238040.1"
                     /locus_tag="LL065_RS18610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18585"
                     /product="AI-2E family transporter"
                     /protein_id="WP_226135684.1"
                     /transl_table=11
                     /translation="MKIDKKLLQYCLYGAGTVLLIYMGITIFNDIGAIFSFLSSFIGKI
                     IGLVKPLLMALVIVYLFKPGVKTIENFLQKRKIFKRAAPRRLVGILAIYLLVIAGVAAL
                     ISGIYIMIGGKLSNNITISNMTEYLTKYLNNPALSVSSITEKLQNSNISMLGNLNDKIT
                     KVVGYVQTYFTTSIGVMTDSVLSIGGNIASFFIALVLSIYLIQDSEYFMDLWEKTFNLI
                     FRKSKMGNKLKEILSIIDITFYKYIRGQLLEACIVGVLSAIVLYFVGIDYALIIGIIAG
                     ICNMIPYIGPVVGTILAVIIALLSGQPIRAVWAVIGMIVVQEVDNKLLAPKIVGDSVGL
                     HPVFTMLAIIIGGSVWGLIGMMIAVPVAACFKVLISKWYTYHMEKTNN"
     gene            7496..8149
                     /locus_tag="LL065_RS18615"
                     /old_locus_tag="LL065_18590"
     CDS             7496..8149
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /locus_tag="LL065_RS18615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18590"
                     /product="YARHG domain-containing protein"
                     /protein_id="WP_226135686.1"
                     /transl_table=11
                     /translation="MYCSKCGSEIKDGANFCFKCGNKNTKQSIINPENISTIPLATTSK
                     EINASNEMTTSMASIKNQPNNTKKFIIIAVVIGVLSIGCISYYISFSKFSPTTTKASDT
                     TSIKNEVIPSNKTSNTKSQATTSANKTDSYIFSKSSSENLLESDLSKLVKGDLSLARNE
                     IFARHGFVFKSEPFKSYFNNKSWYKQNSLFKGTDPELNAKELYNIHLILKYENK"
     gene            complement(8229..9932)
                     /locus_tag="LL065_RS18620"
                     /old_locus_tag="LL065_18595"
     CDS             complement(8229..9932)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025: diguanylate cyclase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416295.1"
                     /locus_tag="LL065_RS18620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18595"
                     /product="diguanylate cyclase"
                     /protein_id="WP_226135688.1"
                     /transl_table=11
                     /translation="MGDLKENKQKKIREIVSIVKLAALLFSAIVLLRYFFKGNTIAKVT
                     GDTNYSFISFFVLILILLSVYFIWSMATVEKIDSKYINIVQFIENLVFILFFIIVIFYS
                     GKNTSDYKFLFLFIIITSTLQQGMNQGMLVASISSIFILSLDLIMGAPNVQVNQFFEND
                     LILAGVFILTAWPLGFYVKIENEHIKKLEGLANEDGLTGLYNHRFFYDALEGIMKTSEK
                     ENNLVAMILIDIDYFKIYNDMNGHQMGDEVLKLVAFILKNSFRKDDVVARYGGEEFVIV
                     LPNTSEEDVIKQAEKLRKKIEATYFNGEENQPNGKLTVSIGTSIYPSKAKNAIELFKSA
                     DDALYRAKFFDKNRVESYTSILEELKIDTEGEHIDIITSIKTLISVINAKDKYTYGHVE
                     RVVIYSRLLADKLKVNEKNKKELIYGAYMHDIGKINIPKEILNKNMPLTPDEWEIMKQH
                     SVNGVEIIKSVESLKEIAPLILHHHERYDGNGYPDNLKEKDIPYLVRMLTVVDCFDAMT
                     SSRPYNIRKTYKEAIEELKRCSGKQFDKEIADSFIEVIRENENQIGNIDD"
     gene            complement(10001..10135)
                     /locus_tag="LL065_RS18625"
                     /old_locus_tag="LL065_18600"
     CDS             complement(10001..10135)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356838.1"
                     /locus_tag="LL065_RS18625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18600"
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_226135690.1"
                     /sec_met_domain="TIGR04223 (E-value: 9.1e-12, bitscore:
                     42.9, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFLKKHLFALIAVATTLAATSVASSACYWSLYQPEEPKCLREK"
     gene            complement(10141..10794)
                     /locus_tag="LL065_RS18630"
                     /old_locus_tag="LL065_18605"
     CDS             complement(10141..10794)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616690.1"
                     /locus_tag="LL065_RS18630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18605"
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_226135692.1"
                     /sec_met_domain="AgrB (E-value: 4.2e-40, bitscore: 135.2,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKVSNNIANKVALELSLDDDNREVISYGAFALIQMFFSIILVFI
                     FGFLFHVAIEALIISFTAAIFRKYSGGVHASSPGICTFIGTIVCVGQAILISLLLNFLV
                     DFKLVIILEVVIFTWSYYIICKLVPVDSIAKPIIKQEKRNRMKKGSIILLSVYLGITVF
                     LILLYISSGEKKLMSYVLCLYSGILWQTFTITSPGHLLVGKVDHLLNHIIKNRG"
     gene            complement(10778..11263)
                     /locus_tag="LL065_RS18635"
                     /old_locus_tag="LL065_18610"
     CDS             complement(10778..11263)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS18635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_18610"
                     /product="hypothetical protein"
                     /protein_id="WP_226135694.1"
                     /transl_table=11
                     /translation="MLRLNLLEVFLRTLPEEFLVIFTVYAFSKTVINVKRYIISSIFSI
                     IAVCLIRLLPIQYGVHTILSIVVIIILTININKISIIKSIQASIMTTILEFICEVVNVF
                     IIQYIFKADVRYVLNDPSLKILYGIPSLVIFSAIVFTYYAYLAKNKKLKEVIDGESI"
     gene            complement(11377..11943)
                     /locus_tag="LL065_RS18640"
                     /old_locus_tag="LL065_18615"
     CDS             complement(11377..11943)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676935.1"
                     /locus_tag="LL065_RS18640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18615"
                     /product="DUF5317 family protein"
                     /protein_id="WP_226135695.1"
                     /transl_table=11
                     /translation="MIEPILLALIFAKIKGYKIKPLFKSWAIYPVLIFAILYVILEVMI
                     FNNNYTLIKYNSIYHYLYLLTFIILVIRYKLNISAIIGSILIIIGSILNNIVIAANNGK
                     MPVFPTLSYLTGYVKSDSFMKVKDIHILGSGITKMKFLTDIIDVGYCIMSIGDICIRAF
                     VFIILFNTIKVINKTSITKDETLIE"
     gene            complement(12052..13197)
                     /locus_tag="LL065_RS18645"
                     /old_locus_tag="LL065_18620"
     CDS             complement(12052..13197)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1247: acetylornithine deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF006365.0"
                     /locus_tag="LL065_RS18645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18620"
                     /product="ArgE/DapE family deacylase"
                     /protein_id="WP_226135878.1"
                     /transl_table=11
                     /translation="MKEAIDFLKDLLKINSSNPPGNEIMVAMAIAKQLRESGIESTIQP
                     VGNNRANLIARIKGTGHKKSLVFCGHMDTVSVGEIPWEHEPFGAEEIDGRIYGRGSSDM
                     KGGLAALIMAMIEVSKSGITLEGDLVFAATAGEEVDCIGANTMVADGSLNGAGAMVIAE
                     PSGGEIFVTHKGALWLEVISYGKTAHGSMPEHGINAIVNMNYFINALHDKFKFKYKEDS
                     LLGGPTLNIATISGGVQVNVVPDMCKLQIDIRTVPGQNHQEILSDIKALLAEIESSSKA
                     KFDIKVFNDKVALKNKSDDQFIKLTLDTAMELFGSRYKAKGVTYCTDASIFVPSFSNNL
                     SVIICGPGEETQAHKPNEYVKTSKYIDSIKLYKEIALRYLK"
     gene            13515..13898
                     /locus_tag="LL065_RS18650"
                     /old_locus_tag="LL065_18625"
     CDS             13515..13898
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783192.1"
                     /locus_tag="LL065_RS18650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18625"
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="WP_226135696.1"
                     /transl_table=11
                     /translation="MADASKISNSEWEVMKCIWGNPTCTANEIVDALSKRTDWKANTIR
                     TLINRLVKKEVLGYNIDKKDKKTYYYFPLLSENECIKAESDSFINRVFNGSLNGMIANF
                     LNESKLSDEEIKELKNILDKKKG"
     gene            13911..15722
                     /locus_tag="LL065_RS18655"
                     /old_locus_tag="LL065_18630"
     CDS             13911..15722
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /locus_tag="LL065_RS18655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18630"
                     /product="M56 family metallopeptidase"
                     /protein_id="WP_226135697.1"
                     /transl_table=11
                     /translation="MLFLISIFKWVLYSTALASILSLIILLFKLLLKNKLGVRWHYAIW
                     LIVVIRLLIPYGLQSSLSIFNIINFENTTITQVPYTITYKANKIFNNKESNTTVGPNLV
                     KSNLQANTSDADQIPIYLQLISCLWLLVVIIIASYTIVMTMKLKSKFKKGHVCNNKKVL
                     LLLLQCKNKLNIKSNIRIINTNEIKTPCIYGFINPKLLLPIGTYNFLNKNELEFIILHE
                     LAHVKRLDIIVSCISGALQIIHWFNPIIWYSFYKMSNDKELACDALALSHVNCENYMNY
                     GKTIIKLLENYKKTPQVYGMSYIINDKSQIKRRITMISLFKKNSYKWSIIPIVTLTLLG
                     GIMLTNAKNKPVLAGNKNSQSINSNKTLSDQDKKSSITQYDIKGKHFNGTMLIIPNDKK
                     IVVGFNKDISKVTKTTSQIAKESNALVAINAGGFTQDVSGKTLAPSGLIMHDGKVIFND
                     IADNNIKGDIVAFTNKGTLLAGKYSLNELNKLNTTEAVSFGPALIVNGKPTITEGDGGW
                     GIAPRTVIGQKKDGSVILLTIDGRSVKSLGATLLDVQNILLQYGAVYASNLDGGSSSTM
                     YYNGKVINNPCDISGERKVVSTFMVLP"
     gene            complement(15850..16104)
                     /locus_tag="LL065_RS18660"
                     /old_locus_tag="LL065_18635"
     CDS             complement(15850..16104)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS18660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_18635"
                     /product="hypothetical protein"
                     /protein_id="WP_071611641.1"
                     /transl_table=11
                     /translation="MSKKNKIYEITISEDGLENYKGQDISAKDMAEDLGIDSVMTDFQW
                     DEDLKKGVILTGRILDSISLEDAFEELENAEFIEDVVES"
     gene            complement(16208..16864)
                     /locus_tag="LL065_RS18665"
                     /old_locus_tag="LL065_18640"
     CDS             complement(16208..16864)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826517.1"
                     /locus_tag="LL065_RS18665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18640"
                     /product="DUF975 family protein"
                     /protein_id="WP_226135698.1"
                     /transl_table=11
                     /translation="MPQQSSDLKRRAREQLNGKWLQAGIVCFIAWLVTMSFSGGNVVHS
                     VQNIWQNGELVRVPMTYSSNGFGNLVSFVLSGPITLGVSAYFLKLIRNEGPVIENMFGG
                     FKFFVKSFVLNFLIGLFTFLWMLLLIIPGIIALLGYSMSYYIMMDNPELSASESLDRSK
                     RMMVGFKFKLFSLWCSYIGWFILGILTFGIGFLWINPYYETAKANFYQDIRFNII"
     misc_feature    complement(17064..17078)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(17098..18042)
                     /locus_tag="LL065_RS18670"
                     /old_locus_tag="LL065_18645"
     CDS             complement(17098..18042)
                     /EC_number="3.1.26.11"
                     /GO_function="GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA]"
                     /GO_process="GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958120.1"
                     /locus_tag="LL065_RS18670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18645"
                     /product="ribonuclease Z"
                     /protein_id="WP_226135700.1"
                     /transl_table=11
                     /translation="MVDLALLGCGGGMPIPERYLSSLLISYKGRKILMDCGEGTQVSMK
                     ILGWGFKSIDVICLTHGHADHIVGLPGLLLTIGNSGRTLPITIIGPIGITAIVKGLMVI
                     APYLPYEINILENPQEPVKIKAPLNATETTVGEFNDLIISTLELEHSTNCLGYSLYFNR
                     KPKFDVKSAIENEIPKEFWNRLQKNEVIMDHGKTYFPKLVFGAERVGIKISFITDTRPI
                     SKIKAFINGSDLFVCEGTYGDENDIEKAIRNKHMTFSEAAKLAVGGEVQELLLTHFSPS
                     MLSPEEYKDRSEVIFKNTIIGTDRKIQTLSFKD"
     gene            complement(18084..18425)
                     /locus_tag="LL065_RS18675"
                     /old_locus_tag="LL065_18650"
     CDS             complement(18084..18425)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456718.1"
                     /locus_tag="LL065_RS18675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18650"
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="WP_216206454.1"
                     /transl_table=11
                     /translation="MADLPNCPKCNSEYTYEDRDLFVCPECANEWTLGSETENSEEKKI
                     IKDANGNVLNNGDSVTLIKDLKVKGSPTSLKKGTKVKNIRLVDGDHNIDCKIDGFGAME
                     LKSEFVKKI"
     gene            complement(18660..18866)
                     /locus_tag="LL065_RS18680"
                     /old_locus_tag="LL065_18655"
     CDS             complement(18660..18866)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS18680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_18655"
                     /product="hypothetical protein"
                     /protein_id="WP_226135702.1"
                     /transl_table=11
                     /translation="MSQNKSIAESNQNIIPNSDNSLSKNMSIKQKDQSIVDNSLNSKNS
                     NNGYEVNGNAGIQAAQQGTRNKR"
     misc_feature    complement(18832..18847)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 19.35"
                     /tool="antismash"
     gene            complement(18923..19720)
                     /locus_tag="LL065_RS18685"
                     /old_locus_tag="LL065_18660"
     CDS             complement(18923..19720)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /locus_tag="LL065_RS18685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18660"
                     /product="Ig-like domain-containing protein"
                     /protein_id="WP_226135704.1"
                     /transl_table=11
                     /translation="MEAFIPGAIEYNPFPVKQVTLDKTSTTVEVGDTVALKVTITPINA
                     TITGVKYTTSDSTIAKIDSSNGIITGVKAGTTTITVTTLDGNKTATCEVIVTKNTKLIN
                     NYGFELGLNDWTVTGDVAAVDLEKDLHSGNSALHYNDTIKNFTVSQTIKGLANGSYELS
                     AWVSGSGGEATSQIYAQTSDKTTLTTNFTNSGWRVWTKPTINNIKVTDGTLTIGAKYVA
                     TGGQWGNIDEFKLIKNNDISLNDLNIDLIKIVNFVKCAFFILV"
     gene            complement(19663..20760)
                     /locus_tag="LL065_RS18690"
                     /old_locus_tag="LL065_18665"
     CDS             complement(19663..20760)
                     /GO_function="GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /locus_tag="LL065_RS18690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_18665"
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="WP_226135706.1"
                     /transl_table=11
                     /translation="MFKNRFKSVLSKVLCTTLVLGISSNFHAIMKVQAEAASTSTFAKG
                     ADVGWLSQMEATNYIFKDKDGNVKDCLSILKDYGIDSIRLRSWVNPSDSKTDGHCSTSE
                     VVAMAVKAKAMGFRIMIDFHYSDTWADPGKQVKPLAWKDHDFGVLKTDVYDYTLGVMNA
                     LKTAGVTPEWVQVGNETNPGMMLPDGSIKDYSKLAQLINRGYDAVKAISNNTKVIIHIA
                     DGYDNVNFRKYFDGLKANNAKYDVIGMSYYPYWAGTDYTSNIDALGNNLNDMAARYGKE
                     VMVVEVGGLDTEPINTYNMLVAVQAKVKAVPNSKGTGVFYWEPEGAKSWSGYALSAWGL
                     DGKPTKLWKLLYLVQLNITHSQLNK"
ORIGIN
        1 agcaaggcat tagatttaat atatttcaat taaatcaaac ctatacagga gaagattata
       61 gactaaacat agggccaaaa ggtttcactg gtgaaaaata tggtggtggc acctactggg
      121 atactgaagc ttactgcatt cccttctatt taagtacagc aaaggaaaaa gtatcaaaga
      181 atcttttatt atatagatat aaccaattag gcaaagccat agaaaatgct aaaaaattag
      241 gttttaaaaa tggagcagcg ttatatccaa tggttacaat gaatggagaa gaatgccata
      301 acgagtggga aataactttt gaagaaatcc atagaaatgg agccatagct tacgctatat
      361 ataattacgt aaactacact ggcgacaagt cttaccttta tgaatttggt cttgaggtgc
      421 ttatagctat atcaaggttc tgggctcaaa gggcaacttt ttcaagcaaa aaaaataaat
      481 atgtaatttt aggtgttact gggcctaatg aatatgaaaa taatgtaaat aataattggt
      541 atactaataa aattgcctgt tggacgatgc agtataccct taaggttata aattatttaa
      601 gtgaacatga aaataaaaca ttagatgaac taaaatcgaa ggttaatttt aaggatgatg
      661 agataattaa ttggaataat ataattaaga acatgtacta tcctagcaat gaagaacttg
      721 ggattttcct tcaacaagat ggttacatgg ataaagagca aatattagtt aaagatctag
      781 ataaaaaaaa tataccaata aatcaaaact ggtcatggga cagaatactt cgctcatgct
      841 ttataaaaca agctgatgta cttcaaggta tttatttatt tgagcaggaa tatgacatag
      901 ccacaattaa aagaaatttt gatttctatg aaccaagaac cgtacatgag tcgtccttgt
      961 ccccatgtat tcatacaata ctcgctgcta aaattgggaa tcatgaaaaa gcttatgata
     1021 tgtatttaag ggcatctaga cttgaccttg ataattataa taatgacacg aaagacggct
     1081 gtcatacaac tagcatggct ggaacttgga tggcagtagt acatggtttt ggtgggcttc
     1141 gagttattaa taataagtta attttaaacc catttattcc agatcattgg tgctcatgtt
     1201 ccttcaaaat aatgtttaga ggtgcacttt taaatattaa atttactaaa gaaacccttg
     1261 aaatacaaaa tgagaatgaa atacccgtaa acttgttagt ccatgaaaat gattattatt
     1321 taaatggaaa taacttatta gaaattaaac tatcataaat ataaattctt ttctacaaaa
     1381 taaattatat tacaaaaggt atagcaaaac tctgctatac cttttcttaa ttttctctat
     1441 ataagattac tggcatttat taattttttt cattagttct tctattttct ttgccgggat
     1501 cggtttataa tagtaataac cttgtatttc atcacaccct tcttttttca aaaattctat
     1561 ctgctctttg gtttccaccc cttcagcaat cacctttaat tctaagttct tagctaacgc
     1621 aattataacc ttaattattg cttcatcttt attgtttata tttatacctc taacaaacga
     1681 aatatctatc ttaattttat caactggtaa ttgtttaata taatttaagg aagagtattc
     1741 cgtaccaaaa tcatctattg caatttttac cccaagttgt tttaattgct ttaaagattc
     1801 aatgatgtac tctgtctcct tcatgattat attttctgtt atctctattt ccaaatcatt
     1861 tggatctaaa cctgtttcat ttaaaattgt aataatatcc tcaattattt ttgtgttttg
     1921 aaactgattc actgaaatat taactgctat aggtacattt aaaataccct tatctttcca
     1981 ttctttattt tgtttacatg cagttttaag tacccattca cctattggca atatcaaacc
     2041 tgttttttct gctatatgta taaaatctat tggatttacc attcctagtt ctgtattgtt
     2101 ccatcttatt aatgcttcca acccaattat tttacctgtt attaaactaa tttgtggttg
     2161 ataaaatagt tctagttcat ttctctctat tgccccaaat aaactctttg ttaacctcat
     2221 ctcttcaact aaactttcct taataatttg agtacaaagc tcaaatttgt tcctaccttt
     2281 ttcttttgcc ttatacatcg cgatatcagc attttttatt aatgtttcta catcactacc
     2341 atctattgga taaatggacc caccaatact agtagttata tataaatcag tattatctat
     2401 tataaaaggt tttttaagat cagtaagtat tttttctgat acagctttaa tatcttcgtt
     2461 gttttttata ttttgaatca gaataagaaa ttcatctcct ccaaccctac aaactgtatc
     2521 actttccaac attgtatttg ttaacctctt tgaaaccatt tttaaaagtt catcaccttt
     2581 tgcatgtccc attgtgtcat ttatcctttt aaaggaatct aaatcaagga ataatactcc
     2641 taggaaactt ttacttagat ccgcattaaa aatcccctga tttaatctat caataaataa
     2701 tgttctattt ggtaaccccg ttaagaaatc ataatatgct aattttttaa ttttaaattc
     2761 catatctata cgtgaactta tttcacgctc gagttcttca tttgaactac ttaactccct
     2821 agttctctcc cccaccttaa tttcaagttc atcatatgat ttttttaatt tttgctgaac
     2881 aagtttaatt tcagatatat cctgaaaaat gcaaacaatc cccagtgaat ctccccactc
     2941 atcctttaag actgaagaag ataaaagtat tgataattta ttattgttac tttgtaaaac
     3001 ttcaatttca aaactactac agtttgttaa tttagataat tctattgtat ctgcgaataa
     3061 ataatttata ggtttattaa ttatcctact tttatcataa cctaataatt taagcgcccc
     3121 tttattaata tcttttataa taccctcatg atttgcaata attaaccctt cactcattat
     3181 tttcaaaacc tctagtgcaa aattttcatg attaaggtcc attaatttgt attttttaat
     3241 agagtaccat actccaatta caggtattac aataaatata ataccaataa acggcataac
     3301 tggccttcta atcattggaa ttacgctatc tgtaattcca cctgcaacaa ctgcaataaa
     3361 agttgttacc aatataagtt ttgcctgttt tctatccctt actatttctg aattcctcca
     3421 ccatttaaat aataagaata taactgaaat tatataagaa aaccaatata tattgaaaaa
     3481 attcgaccaa ataaatccct tattttttga tgcaatataa gcccaaccta aatttgtttc
     3541 aacaaagttt tgcgatgttt ctgtatggaa aacataaaga taaatagata ttattgctgg
     3601 taaatataaa attatataag cgaatttctt tttaaaaaaa acgcctttat tagttaaaat
     3661 aattataaaa tttaaaaata tacaataaaa aatagaccaa aaaaatgtag aatataaata
     3721 aaaatcactt gcagctttag cattccttga acaattcatc aagaagaaca taaacgacca
     3781 taatgccaaa tttatggcca ttacagaaaa taatttattt aattcatttt ttgaatcatt
     3841 taaaaatgta ataataccca aaatcacata tgtaattcca cataaaaaat ataaaattgt
     3901 taatatagcc atgtattatt ctcccccagt tagtcatttt tctaattcat ttataaagta
     3961 taccataata cttacaaatt ttatctatac ttatctttta aataagcaaa actccccttc
     4021 ttaagttaag aaagggagtt ttgttgtatt aattaacaga ttaattataa ttagatatta
     4081 aaatttctgt gctactttcc ttgcttcgtt tatagcattt atctttattt catctgcttt
     4141 atcaggagcc gaattcatgc cttctacaat aattgattgt ttatttgtaa tcccaataaa
     4201 atttaatatt gtatttatat atctgtctcc taattcaaga tcagcagcga gcccaacaga
     4261 ataaacgcca cctctagcct gaatatggac tgctttttta tcagttaaaa gtccaatagg
     4321 accattttgt gtatatttaa atgttttatt aactatacat atattatcta aatacgcttt
     4381 catcattgga ggtattgtga aattccaaag tggtgtaaca aatacatatt tatcagccgc
     4441 tataaactgg tctaataaat tattcatagc aaccacttta ttctgttctt ctttatctaa
     4501 cttttcaaat gtaactcctg ctccaagttt tccccaagca ctgaatacta cctcatcaat
     4561 aaatggaacc tctgttttat ataaatctaa agttattatt tcatcagatg gattagattt
     4621 tttataagct tctaaaaatt catttcctac agctaaacta aacgaatttt ccttagtttt
     4681 tggatttgca gttatatata atatttttga catttttaca ttcccctttt cactctttaa
     4741 tatattttta aattttaata attagtattg ttttaatatt aattttatat gctctaattt
     4801 ttttgttaca taaagtaact cgtatacttt ttgtaattca attactttat gttgttataa
     4861 tacattaata tacttacttt tgtcaagtaa gtatgttaaa ataattatag gagatgattt
     4921 agcatggata aatttcattt atgtccaaga tttgaggcag cttttcaaat tttaggaaaa
     4981 cgatggactg gattaattat acgttcctta ttaagtggtt caaaaagatt ttcagagcta
     5041 caagagatta ttccagcttt aagttctcgt gtacttacag aaagatttaa agaattagaa
     5101 gaactaggca ttgttactcg tagtgtttac cctgaaatgc ctgtacgtat agaatatgga
     5161 cttaccgaga aaggcaagga cttagaaaaa actatggatg aaatccaaaa atgggcagag
     5221 aaatggcccg ataagtaatt atttttatat accctctaaa aaattagagg gtatatttat
     5281 tttttatcat ataaacatag taaacatatc attttattaa caactttatc taaaaaaata
     5341 taatttatta ttgacttcat gaaattaata aagtagaatg gttttgataa tcattatcaa
     5401 ataagaagtt aacagaggag tttttacatg atttttatct attctttaat tttcgtttta
     5461 attctatcgt tattttttac atcaaacatc aaaaaaaatt attggactta ttatttaatc
     5521 tctatattta ttggaatcat tactactgtt tatgaagttc ttagaataac ttccgatgtc
     5581 aaactttatg gatttcctct cttcctagaa aaaatattta taagaggtta tatttccata
     5641 gcatttttca ttttagtaat gtttgcaggt gctctaaatc ctaaatggaa aataacaaga
     5701 aaactccaaa gcataagggc ggaactcgcg attatagcct ctatctttat aatacctcat
     5761 gctcttattt attttgttcg ttttatcgta cttaaacttc caaaaatgtt tagcacaggt
     5821 gtaataccaa aattatatat agcttatatt attgctggga ttatagcatt tgttataatg
     5881 attcctttgt ttattacctc atttaaaagg atcaggtcta aaatgcaaag tggaaagtgg
     5941 cgaaaattgc aaaagcgagc atatatattt tactttttag tgtacgtaca tatcctgatt
     6001 ataatgttaa atgaaaaaga aattgatcta ttaaaattaa gtagttacac aattatattt
     6061 ggtacataca caattttaag gttaattaaa tataaaaata atagacaatc taaattaaaa
     6121 attgctagtt aagttatctc ctgcggagct gcaatatgaa aactaataac taaatataat
     6181 atttagttat tagttttctc catatgatag gtgtaccatt tgctaattaa tactttaaaa
     6241 caagcagcga ctggtaccgc tatcatcata cctataagtc cccatacact tccacctatt
     6301 ataattgcaa gcatagtaaa taccggatgt agtcctacac tgtctccaac aattttcggt
     6361 gcaagtaatt tattatccac ctcctggact acaatcatgc caataactgc ccatactgct
     6421 ctaataggtt gtccacttag cagtgcaata attactgcga gaatagttcc aacaacagga
     6481 cctatatatg gtatcatatt acaaatacct gcaataattc ctattattaa agcataatcg
     6541 attccaacaa aatacaatac gatagctgat aaaacaccta caatgcaagc ctctaataat
     6601 tgacctctta tatatttata aaaagtgata tcaataattg ataaaatttc ttttaattta
     6661 tttcccattt tacttttcct aaatatgaga ttaaaagttt tttcccataa gtccataaaa
     6721 tattccgagt cttgtattaa atatatactc aatactaaag ctataaaaaa tgaagctata
     6781 ttgcccccta tagataaaac agaatctgtc attactccta tacttgtcgt aaaatatgtc
     6841 tgaacataac ctacaacttt agttatttta tcatttagat ttcctaacat tgaaatattt
     6901 gagttttgta atttctcagt gattgaacta actgacaatg ctgggttatt taaatattta
     6961 gtaagatatt ctgtcatatt acttatagtt atattattag aaagctttcc accaatcata
     7021 atgtatattc cactaataag agcggcgaca ccagctatta ctaataaata aatcgctaaa
     7081 attcctacta atcttctagg agcagctcgt ttaaatatct ttctcttttg caagaaattt
     7141 tcaatagttt tcacacctgg cttgaacaag tacacaatta ctaatgccat aagaagtggt
     7201 tttacaagtc caataatttt tcctataaaa cttgataaaa aagaaaatat agcacctata
     7261 tcattaaaaa ttgttatacc catataaatt aaaagcactg tccccgcgcc atataaacag
     7321 tattgtaata attttttatc tattttcatt tgtatcccct ttcttctata gctacttact
     7381 ttataacttt agcataaatc cgccataaat tcattgtttt tattttaatc ctttaaaagc
     7441 cacatttatt ttagtataat atctattata aagcttaaag aggagacggt gatttatgta
     7501 ttgttctaaa tgtggttctg agataaaaga tggcgccaat ttttgcttta aatgtggtaa
     7561 taaaaacaca aaacaaagta taataaatcc tgaaaatatt tcaactatac cactcgccac
     7621 tacttcaaag gaaattaatg cttcaaatga aatgactact tcaatggcaa gcatcaaaaa
     7681 tcaacctaat aatactaaaa aatttattat aattgctgtt gtaattggag tattatcaat
     7741 aggatgtatt agctattata tttcattttc taaattttca cccacaacta ctaaagcttc
     7801 agacacaact tctataaaaa acgaagttat tccaagtaat aaaacctcta atacaaaatc
     7861 tcaagctact acatctgcaa ataaaactga ttcttatatt ttttctaaaa gcagtagtga
     7921 aaatctttta gaatctgatt tatctaaatt agtaaaagga gatttatctt tagctagaaa
     7981 tgaaatcttt gcaagacatg gttttgtatt taaaagtgaa ccttttaaga gctatttcaa
     8041 taataagtct tggtataaac aaaattctct ttttaaggga actgacccag aattaaatgc
     8101 aaaagaatta tataatatac acttaatttt aaaatatgaa aacaaataac aaaattcaga
     8161 aaatgctgat aaggttatcc acattttctg aatttcttgt gattttagaa tagataaaaa
     8221 aatatcttct aatcatctat attaccaatc tgattttcat tttccctaat aacttcaata
     8281 aaagaatccg caatttcttt atcaaactgc ttaccactac atcttttaag ctcttcaata
     8341 gcttctttat aagtttttct tatattatat ggtctagaag acgtcatagc atcaaaacaa
     8401 tctactactg tcaacatcct cacaaggtaa ggtatgtctt tttcttttaa attatctggg
     8461 tatccattac catcgtacct ctcatgatga tgtaatatta gaggagcaat ttccttaagt
     8521 gattcaacag attttataat ctctactcca tttactgaat gttgtttcat tatttcccat
     8581 tcatctgggg ttaatggcat atttttattt aatatttcct ttggaatatt tatttttcca
     8641 atgtcatgca tgtaagctcc atatattaat tccttcttat ttttttcatt tactttaagt
     8701 ttatcagcga gtaacctact ataaataact accctttcga catgaccata tgtatattta
     8761 tccttagcat taataacact tatcagagtt tttattgatg taataatatc tatatgttca
     8821 ccttctgtat caatttttaa ttcttccaat atagaagtat aactttcaac tctattttta
     8881 tcaaaaaact tagccctata aagtgcatca tccgcacttt taaaaagttc aatagcattt
     8941 ttagctttac ttggataaat tgatgttcct attgagacag tcaattttcc atttggttga
     9001 ttttcttcac cattaaaata ggttgcttct atcttttttc ttaatttttc tgcttgttta
     9061 attacatctt cttcagaagt attgggaaga actataacaa attcttcacc cccatatctc
     9121 gcaactacat catcttttct aaaactattt ttcaatataa aagcaactaa cttaagtact
     9181 tcatcaccca tttgatgtcc attcatatca ttataaattt taaagtaatc tatatctatt
     9241 aaaatcatcg caactaaatt attctctttt tcactagttt tcattattcc ctctaatgca
     9301 tcataaaaaa atctatgatt ataaagccct gtgagcccat cctcatttgc taatccctcg
     9361 agctttttta tatgttcatt ttctattttc acataaaaac ccaatggcca tgcagtaagt
     9421 ataaaaactc cagctaaaat taggtcattc tcaaaaaact gattaacttg tacattaggt
     9481 gcacccataa taagatctaa tgataatata aaaattgaag aaatgcttgc cactaacata
     9541 ccttggttca taccttgttg cagcgttgaa gttattataa taaataaaaa tagaaattta
     9601 taatcacttg tatttttacc tgaataaaat ataacaataa taaaaaatag aataaataca
     9661 aggttttcta taaattgtac aatattaata tatttagaat ctattttttc aacagtcgcc
     9721 attgaccata taaaatatac cgacaataat attaatatta atacgaaaaa tgaaataaaa
     9781 ctataattag tatctcctgt tacttttgct atggtattac ccttaaaaaa atatcttaga
     9841 agtactattg ccgaaaacaa taaagcagct aattttacta ttgacactat ttctcgaatt
     9901 ttcttttgtt tgttttcttt taaatcaccc atattatcac tacctcatta aattcattat
     9961 aataaataaa aaaggtcagt aaactgacct tttttattta ttatttttct cttaggcatt
    10021 ttggttcttc aggctgataa agagaccaat aacacgccga agacgccaca gaagtcgcag
    10081 caagtgtagt tgctactgca attaaagcga ataagtgttt ttttaaaaat ttcataatat
    10141 ttacccccta tttttaatta tatgatttaa aaggtgatct actttaccta ctaataagtg
    10201 ccctggagac gtaatagtaa aagtttgcca taatatgcct gaatataaac ataaaacata
    10261 agacataagt tttttttctc cactacttat atataacaag ataagaaata ctgttatacc
    10321 taaataaaca cttaaaagta ttatagatcc ttttttcatc cgatttcttt tttcttgttt
    10381 tattatgggt tttgctatgc tgtccaccgg aactaattta caaattatat aataagacca
    10441 agtaaaaatt acaacttcaa gtattataac taatttaaaa tcgactaaaa aatttaataa
    10501 aagtgatatt aatatcgctt gtcctacaca cacaattgta cctatgaagg tacaaattcc
    10561 tggggaactt gcatgaaccc ctccagaata ctttctaaaa atagcagctg taaatgatat
    10621 tattaaagct tcaatagcta catgaaacaa aaatccaaat ataaaaacaa gaattataga
    10681 aaaaaacatc tgtattaaag caaaagcccc gtatgagatt acctctctat tatcatcatc
    10741 tagggataat tctaatgcta ctttattagc aatattatta gatactttct ccatctataa
    10801 cctccttcaa ctttttattt ttagctaaat aggcataata tgtaaatact atagccgaaa
    10861 aaatcaccaa tgatggaata ccatataata tttttaatga aggatcattt aacacatacc
    10921 tcacatctgc tttaaatata tactgaatta taaaaacgtt cactacttca cagataaatt
    10981 cgagaatcgt tgtcataata ctagcttgta ttgacttaat tatactgatc ttatttatat
    11041 taattgtcaa tataataatt actacaatac taagaatagt atgtacccca tattgaattg
    11101 gtaataacct tattaaacaa actgcaataa tagaaaatat acttgatatt atatatcttt
    11161 tcacattaat aacagttttt gaaaaagcat agactgtgaa aataactaaa aattcctccg
    11221 gcagagttct taaaaacact tctaataaat tcaatctcaa catttcgtat ttttctccta
    11281 acaatcctac agattctttt gtatatattg tcaattatat cattaatatt acaaaacagt
    11341 atatatttat taatatatct caactaattt tatatactat tcaatcaaag tttcatcctt
    11401 tgttattgat gttttgttaa taacttttat agtattaaat agaatgataa aaacaaaagc
    11461 tcttatacat atgtctccaa tactcataat acaataacct acatcaatta tatctgttaa
    11521 aaacttcatt ttagtaattc cgcttcctaa aatgtgtata tccttaacct tcataaaaga
    11581 atctgatttt acataaccag ttaagtatga taatgttgga aatacaggca ttttcccgtt
    11641 attggcagct ataacaatat tatttaagat acttccaata ataataagta ttgaccctat
    11701 aattgcacta atattaagtt tatatctgat aaccaagatt ataaaggtta aaagataaag
    11761 ataatgataa atactgttat attttataag tgtataatta ttattaaata tcataacctc
    11821 tagaattaca tacaatattg caaatattaa tacagggtat atcgcccagc tcttaaaaag
    11881 tggttttatt ttgtaacctt ttatctttgc aaaaataagt gctaaaagta ttggttcaat
    11941 catatatatt cctcgcattc acaattttta catttagttt aatttatctc tattatacta
    12001 atatttagca tgtatttcta ccttttcaca aaaaaaattg tgtttttttt attattttaa
    12061 atatcgtaat gctatttcct tgtataattt aatagaatca atatatttac ttgtttttac
    12121 gtattcgttt ggcttatgag cttgcgtttc ttcgccaggt ccacaaataa taacagataa
    12181 attattacta aaactcggga caaatatgga tgcatctgta caataagtta cacctttggc
    12241 tttatatcga cttccaaaca attccatcgc tgtatctagt gttaacttaa taaattgatc
    12301 atcggatttg ttttttaatg ccactttatc attgaaaact ttaatatcaa atttagcttt
    12361 agaacttgac tcaatttctg ctaataatgc cttaatatct gatagaattt cctgatgatt
    12421 ttgacccgga acagtacgaa tgtcaatttg caatttgcac atatcaggca ctacattgac
    12481 ttggacacca ccagaaatag tagcaatatt taaagttgga cccccaagca ggctatcctc
    12541 cttatatttg aatttaaact tatcatgcaa agcattgata aaatagttca tgttaactat
    12601 tgcattaatt ccatgttcag gcattgaccc atgagcagtt tttccatatg aaattacctc
    12661 tagccacaag gcccctttgt gggtaacaaa aatttcacca ccactaggct cagcaatgac
    12721 catagctcca gcaccattta agcttccatc cgctaccata gtatttgcac ctatacaatc
    12781 aacctcttca cctgcagtcg ctgcaaaaac caggtcccct tccagtgtta ttcctgattt
    12841 agaaacctca atcatagcca taattaaagc ggctaaccca cctttcatgt cagaggatcc
    12901 acgcccataa attcgaccat caatttcttc agctccaaat ggttcatgtt cccatggaat
    12961 ttctcccact gacacagtat ccatatgacc gcaaaaaact aaactttttt tatgtcctgt
    13021 tccctttatc ctagcaatca aattggcacg gttgttgcca accggctgta ttgtggattc
    13081 aataccactt tcccttaact gttttgcaat agccatcgct accattatct cattaccagg
    13141 tggattgcta ctattaattt ttaaaagatc ttttagaaaa tcaattgcct ctttaattta
    13201 taatcactcc cattataagt ttatatgcca tatctttact acctcttata tttacattcc
    13261 ctaaaaatta tattatatta attctttctc caaacataac atatctcctc tcacatagtt
    13321 ttaattaatt atactctata acaatgcttc ctcatacttt gtttcatcat cttaactact
    13381 aacctattaa ctaaataact ataaaaatat atttataact tatattgaca attaaatatt
    13441 acagatgtag tatatattta tagtacatat tacagctgta atatataaac tttaatataa
    13501 agggtgatga agaaatggct gacgcctcta aaatatcaaa ttcagaatgg gaagtaatga
    13561 agtgcatttg gggaaatcct acctgcactg caaatgagat tgttgatgct ttaagtaaaa
    13621 ggactgattg gaaggcaaat actattagaa ctttaataaa tcgtttagtc aaaaaagaag
    13681 ttttaggtta caacatagat aaaaaagata aaaaaacata ttattacttt cctttattat
    13741 cagaaaatga gtgtataaaa gctgagagtg actcctttat aaaccgtgtg tttaatggct
    13801 cattaaatgg tatgatcgca aactttttaa atgaatcaaa attgtctgat gaggaaatta
    13861 aagaacttaa aaatattctt gataagaaga aaggttaaat aggtgtattt atgttatttc
    13921 taatatctat ttttaaatgg gtattatatt caaccgcttt agcaagtatt ttaagtttaa
    13981 taattcttct ttttaaatta ttacttaaaa ataaattagg cgttaggtgg cactatgcta
    14041 tttggcttat tgtagtaatt agattattaa taccctacgg actacaaagc tcattaagca
    14101 tctttaatat tattaatttt gaaaatacaa ctattacaca agtaccttat acaataactt
    14161 acaaagcaaa caaaattttt aataataaag agagtaatac aactgtaggt ccaaatcttg
    14221 tgaagtctaa tttacaagca aatacatctg atgctgacca gattccgata tatttacaat
    14281 tgataagttg cctgtggtta ctggtcgtta ttataattgc ttcttataca atagtaatga
    14341 cgatgaaatt aaagtccaag tttaagaagg gtcatgtgtg taataataag aaagttttac
    14401 ttcttttatt acaatgcaaa aacaagttaa atataaaatc taatataaga attataaata
    14461 ctaatgaaat taaaacacca tgcatttatg gatttataaa ccctaaattg ctactaccta
    14521 ttggtactta taattttcta aataaaaacg aattagaatt tataatctta catgaactag
    14581 cccatgtaaa acgcctcgac ataatagtaa gttgcatatc aggagcactt caaataatac
    14641 attggtttaa tccaattata tggtattcat tttataaaat gtctaatgat aaggaactcg
    14701 cttgtgatgc cttagctttg tctcatgtta actgtgaaaa ctacatgaat tatggtaaaa
    14761 ctattataaa attgttagaa aactataaga aaacgcctca agtttatgga atgtcataca
    14821 ttattaacga taaatcacaa attaaaagga gaattactat gatctcttta ttcaagaaaa
    14881 attcttataa gtggtcgatt atacctatag taacattaac tttattggga ggaataatgc
    14941 ttactaatgc taaaaataaa ccagtgcttg caggcaataa gaatagtcag agcataaact
    15001 ctaacaagac tttgagtgac caagataaaa aaagctccat tacacaatat gatattaaag
    15061 gtaaacattt taacggtaca atgttaataa taccaaacga taaaaaaatt gttgttggat
    15121 tcaacaaaga tatttcaaaa gtcactaaaa ccacaagtca aatagcaaaa gaaagtaatg
    15181 cactagttgc tatcaatgca ggaggattta cacaagacgt ttctggcaaa acactggccc
    15241 cctctggact tataatgcat gatggaaaag ttatttttaa tgatatagca gacaataata
    15301 ttaaaggaga tatagttgct tttacaaata aaggtaccct tctagctggt aaatattcct
    15361 taaatgagct aaataaactt aatacaacag aagccgttag ttttggacct gcacttatag
    15421 ttaatggtaa acctacaatt acagaaggcg atggtggttg gggaattgct ccaagaacag
    15481 ttataggtca gaaaaaagat ggttcagtaa ttttattaac tattgatggt agaagtgtta
    15541 aatctttagg cgctacatta ctcgatgtac aaaatatact tttacaatat ggcgctgtat
    15601 atgcatcaaa tcttgatgga ggaagttcat caaccatgta ttataacggt aaagtaatta
    15661 ataatccatg tgatatttcc ggagaaagaa aagtagtatc cacttttatg gtattacctt
    15721 aataaaatca cctgtagtta ttgcttaaat aactacaggt gattttatat attactaagc
    15781 taatgactta cggttaatat ctaaatacca tatcatacca ttttgcacta aggctatagt
    15841 ataaactctt tagctttcca ctacatcctc aataaactca gcgttttcca attcctcaaa
    15901 agcatcttct aaagatatac tgtctaaaat cctaccagta agaattacac ctttttttaa
    15961 atcttcgtcc cattgaaaat cggtcattac gctatcaatt cctaaatctt cagccatatc
    16021 ctttgcacta atatcttgac ctttataatt ttctagacca tcttcgctaa ttgtaatttc
    16081 atagatttta tttttctttg acattcatat tcctccctag accataatat aatattttaa
    16141 aaatactaca tctaatgttc tacaaaggac tgaataatat ccctctgttt ttttagggta
    16201 taaaagttta gattatatta aatcttatat cctgataaaa attagcttta gcagtttcat
    16261 agtaaggatt aatccataaa aatcctatac caaaagttaa tattcctaat atgaaccagc
    16321 ctatataact acaccataaa ctaaacagtt taaatttgaa gccaaccatc atgcgtttac
    16381 ttcggtctaa tgattccgag gcacttagtt caggattatc catcataata taatatgaca
    16441 ttgaatatcc taataaagca ataattcctg gaatgataag aagtaacatc cataaaaatg
    16501 tgaaaagtcc aataagaaaa tttagaacaa atgatttcac aaaaaattta aaaccaccaa
    16561 acatattttc aataaccggt ccctcatttc gtataagctt aagaaaataa gctgatacac
    16621 caagagttat tggtccagac aaaacaaaac ttaccaaatt accaaagcca tttgatgagt
    16681 acgtcattgg aactctaacc aattctccat tctgccaaat attttgaacg gaatgtacaa
    16741 cattcccccc tgaaaatgac attgtaacta accaagcaat aaaacacaca atccctgctt
    16801 gaagccattt tccatttaac tgttcccttg ccctacgttt taaatcacta ctttgttgtg
    16861 gcatgttctt aaccccctta ataatttaac taatcgctct taatttacgc tcctatttat
    16921 agtattcaaa tattgtctta aataacccta aaatttaatt ttactatctt atcacatttt
    16981 ttagcaaaac actctgatta catatttcaa tatcattcta caattaccct ttaaatttcc
    17041 taggaaatat caaaaaccac tactcccata agtagtggtt ttgtaaataa tgtttttcta
    17101 atctttaaaa gaaagagtct gtatctttct atccgtaccg ataatagtgt ttttaaaaat
    17161 aacttccgat ctatccttat actcttctgg gctcagcatt gaaggactaa aatgtgttaa
    17221 taaaagttct tgtacttctc cacctacagc aagcttggct gcctcactaa aggtcatatg
    17281 tttattcctt atggcttttt caatatcgtt ttcatctcca taagttccct cacatacaaa
    17341 caagtcgctt ccattaataa atgcttttat tttagaaatt ggccttgtat ctgttataaa
    17401 actaatcttt attcctactc tttcagctcc aaaaaccaac tttgggaaat atgttttccc
    17461 gtggtccatt ataacttcat ttttctggag tctattccaa aattcttttg gtatttcatt
    17521 ttcaattgca cttttaacat caaactttgg ttttctatta aaatagaggc tgtaacctaa
    17581 acaattagtt gaatgctcaa gttccaatgt tgaaattatc aagtcattaa attctcctac
    17641 tgtagtttct gtagcattta atggagcttt tatcttcaca ggttcttgtg gattttctaa
    17701 gatattaatt tcataaggaa gataaggggc tataaccatt aaccccttca caatagctgt
    17761 aatacctata ggcccaatta tagtaattgg aagcgttctg ccactgttac caattgttaa
    17821 aagcaaccct ggaagtccaa caatgtgatc tgcatgtcca tgagttaaac aaatcacatc
    17881 tattgactta aatccccacc caagtatttt catagatacc tgagtgcctt ctccgcagtc
    17941 cattagtatt tttcttcctt tataacttat tagaagtgaa gataaatatc tttctggaat
    18001 tggcatacct cctccacatc ctagaagtgc taaatcaacc aattaaatca cctcctctat
    18061 tttttttgtt tataaaatat tatctatatt tttttaacaa attcagattt taattccata
    18121 gctccaaaac catcaatttt acaatcaata ttgtgatctc catcaaccaa acgaatgttt
    18181 tttacttttg tacctttttt taaggatgtt ggactcccct ttactttaag atctttaatt
    18241 agtgttacag aatcaccatt gtttaataca tttccatttg catctttaat aatttttttc
    18301 tcttcactat tttcagtttc tgatcctaaa gtccactcat ttgcacattc tgggcaaaca
    18361 aaaagatctc tatcctcgta tgtgtattct gaattacatt ttgggcaatt tggcaaatca
    18421 gccataattt catttcctcc attcgttatt gtttctagtg tataattcct tatagatttc
    18481 attgttaaat aatcacctgc ggtgctgcaa cagcttcgca gaagatatat tggtttctca
    18541 ttcgtttata tactatcata gtcttctact atctacaaac catcatgcaa atatatataa
    18601 aaaacacagt agataaaata tatacatatc tactgtgctt tttaatttaa taatttaatt
    18661 tatcttttgt ttctagtgcc ttgctgagca gcttggattc cagcatttcc attaacttca
    18721 tatccattgt tagaattttt actattcaga ctgttatcaa caatgctttg atccttttgt
    18781 tttatgctca tatttttact cagactatta tcactattag gtataatatt ttgattgctt
    18841 tcagctattg atttattttg gctcataaaa aacatctcct ttaataatta ttctatatta
    18901 gaatgccatt aacataatat acttatacta aaataaaaaa tgcgcattta acaaaattta
    18961 ctatttttat taaatctata tttaaatcat taagcgagat atcattattc ttaattaatt
    19021 taaattcatc aatattaccc cactgtcctc ccgtagctac atactttgca cctattgtta
    19081 gagttccatc tgttacctta atattattaa ttgtaggttt cgtccacaca cgccaacccg
    19141 aatttgtaaa attagttgtt aatgtggttt tatctgatgt ctgcgcataa atttgtgaag
    19201 ttgcttcacc accactacca gaaacccaag cagacaattc atatgatcca ttagctaagc
    19261 ctttaatagt ttgagaaaca gtaaaattct ttatggtatc attataatgt aatgcactat
    19321 tcccagagtg taaatccttc tctagatcca ctgctgctac gtcaccggtc actgtccagt
    19381 catttaaacc taactcaaaa ccataattat ttattaactt agtatttttg gtaacgatta
    19441 cctcgcatgt tgcagtctta ttcccatcta gggttgttac ggtaatagtt gttgttcccg
    19501 ccttaacacc tgtaataatt ccatttgaag aatctatttt tgcaatggtt gaatctgaag
    19561 tcgtatattt aacaccagta attgttgcat ttattggtgt tatagtaacc tttaaagcta
    19621 cagtatcacc tacttcaaca gtagtgcttg ttttgtcaag ggttacttgt ttaactggga
    19681 atgggttata ttcaattgca ccaggtataa aagcttccat agctttgtag gttttccatc
    19741 taagccccag gcactcaatg catatccact ccagcttttt gctccttctg gttcccagta
    19801 aaatactcca gttcccttgc tgttgggaac tgcctttacc ttagcctgaa cagctacaag
    19861 catattgtaa gtattaatag gttccgtatc taatccacct acctcaacta ccatcacctc
    19921 tttaccgtat cttgctgcca tatcatttaa attatttccc aatgcgtcta tgtttgaagt
    19981 ataatccgtt cctgcccaat atggataata ggacattccg attacatcat atttggcatt
    20041 attagcttta agaccatcaa aatactttct aaaattcaca ttatcatatc catcagctat
    20101 atgtataata acttttgtat tattacttat agccttaaca gcatcatatc ccctatttat
    20161 caactgagct aacttactat aatcttttat acttccatct ggaagcatca taccaggatt
    20221 agtttcattt cctacctgaa cccattccgg tgtaacacca gcagttttca aggcattcat
    20281 aactcctaat gtatagtcat atacatcagt ctttaagacg ccaaaatcgt gatctttcca
    20341 ggctaatggt tttacttgtt ttcctggatc tgcccaggta tcactgtaat gaaagtctat
    20401 cattattcta aagcccatgg cttttgcctt aaccgccata gcaactacct cagatgtact
    20461 gcaatgacca tcggttttac tatctgaggg attaacccag cttcttagcc taattgaatc
    20521 aattccataa tcttttaaaa tagataagca atcctttaca tttccatctt tatctttaaa
    20581 tatatagttc gtagcctcca tctgtgagag ccagcctacg tctgcgcctt ttgcaaatgt
    20641 actagtactg gccgcttccg cttgtacctt cataattgca tgaaaattac tacttatccc
    20701 caaaacaagt gttgtgcata aaacttttga taaaacactc ttaaacctat ttttaaacat
    20761 taatattccc tccatttata ctctcttatt ttat
//
