LOCUS       NZ_CP086140            22399 bp    DNA     circular CON 20-JAN-2024
DEFINITION  Clostridium estertheticum strain CF016 chromosome, complete genome.
ACCESSION   NZ_CP086140
VERSION     NZ_CP086140.1
KEYWORDS    RefSeq.
SOURCE      Clostridium estertheticum
  ORGANISM  Clostridium estertheticum
            Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5053333)
  AUTHORS   Wambui,J., Stevens,M.J.A. and Stephan,R.
  TITLE     Clostridia strains as spoilage organisms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053333)
  AUTHORS   Wambui,J., Stephan,R. and Stevens,M.J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2021
            Assembly Method                   :: unicycler v. 1
            Assembly Name                     :: CF016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Nanopore/Illumina miniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 05:10:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,249
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 5,019
            CDSs (with protein)               :: 5,019
            Genes (RNA)                       :: 135
            rRNAs                             :: 15, 14, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 13 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 22 of 95
            Pseudo Genes (incomplete)         :: 78 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:27:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3692943
            Orig. end                         :: 3715342
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086140.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="deazapurine"
                     /region_number="5"
                     /rules="(GTP_cyclohydroI and PTPS and QueE and QueC and not
                     (dpdA1 or bTGT))"
                     /tool="antismash"
     cand_cluster    1..22399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(GTP_cyclohydroI and PTPS and QueE and
                     QueC and not (dpdA1 or bTGT))"
                     /kind="single"
                     /product="deazapurine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:12399](+)"
                     /cutoff="20000"
                     /detection_rule="(GTP_cyclohydroI and PTPS and QueE and
                     QueC and not (dpdA1 or bTGT))"
                     /neighbourhood="10000"
                     /product="deazapurine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(GTP_cyclohydroI and PTPS and QueE and
                     QueC and not (dpdA1 or bTGT))"
                     /neighbourhood="10000"
                     /product="deazapurine"
                     /protocluster_number="1"
     gene            complement(719..1885)
                     /locus_tag="LL065_RS17455"
                     /old_locus_tag="LL065_17435"
     CDS             complement(719..1885)
                     /NRPS_PKS="Domain: Aminotran_3 (17-336). E-value: 1.1e-108.
                     Score: 355.1. Matches aSDomain:
                     nrpspksdomains_LL065_RS17455_Aminotran_3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443549.1"
                     /locus_tag="LL065_RS17455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17435"
                     /product="aspartate aminotransferase family protein"
                     /protein_id="WP_226135325.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.2e-120, bitscore:
                     399.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEENCVMNTYGRYDVTFEKGLGCKIYDSVGQEYMDFVSGVAVNCL
                     GHSHPAIINAITTQSSKLMQSSNYYWNTPHKLLAKKLIDNCDHDKVFFCNSGTEAIEAA
                     LKIARKYGALKTTKDKNVLIYMDESFHGRTMGSLSVTGQEKYQKDFRPLIGGTQKVEFN
                     DVEELKKNFNDKVCAVIVEPIQGEGGINIATIEFLKAARDLCDKYDALLIFDEVQCGMG
                     RLGSLFAYKKFSVIPDVICIAKALGGGFPIGATITNVKASAAFVPGDHGSTFGGNPLAC
                     AVGEAVLTELLDGGVIEKIDDKSAYMKERLLKIKEKYGVIDEIKGIGLLVGIKIKTDTK
                     KFCKSCFDKKLLVIAAGNNVVRLLPPLNVTKGEIDEALDILERVISEI"
     aSDomain        complement(878..1834)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LL065_RS17455_Aminotran_3.1"
                     /evalue="1.10E-108"
                     /label="LL065_RS17455_Aminotran_3.1"
                     /locus_tag="LL065_RS17455"
                     /protein_end="336"
                     /protein_start="17"
                     /score="355.1"
                     /tool="antismash"
                     /translation="EKGLGCKIYDSVGQEYMDFVSGVAVNCLGHSHPAIINAITTQSSK
                     LMQSSNYYWNTPHKLLAKKLIDNCDHDKVFFCNSGTEAIEAALKIARKYGALKTTKDKN
                     VLIYMDESFHGRTMGSLSVTGQEKYQKDFRPLIGGTQKVEFNDVEELKKNFNDKVCAVI
                     VEPIQGEGGINIATIEFLKAARDLCDKYDALLIFDEVQCGMGRLGSLFAYKKFSVIPDV
                     ICIAKALGGGFPIGATITNVKASAAFVPGDHGSTFGGNPLACAVGEAVLTELLDGGVIE
                     KIDDKSAYMKERLLKIKEKYGVIDEIKGIGLLVGIKIK"
     gene            complement(1885..2760)
                     /gene="argB"
                     /locus_tag="LL065_RS17460"
                     /old_locus_tag="LL065_17440"
     CDS             complement(1885..2760)
                     /EC_number="2.7.2.8"
                     /GO_function="GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="argB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1174: acetylglutamate kinase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616224.1"
                     /locus_tag="LL065_RS17460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17440"
                     /product="acetylglutamate kinase"
                     /protein_id="WP_220707120.1"
                     /transl_table=11
                     /translation="MDILNNESNKKDFLQMSKYEGKTFVIKYGGSIMSNDVAKEAFVED
                     IELMTKAGIKVVIVHGGGPLISKWVKKSGGENKFIEGLRVTSKETMEIVEMVLSGNVNK
                     RLSSILSLRGLNAIGISGRDSNLIQSKKKYVYEDGRKIDIGFVGEVANINTKVLLTLLK
                     NKYIPVISPVGVGCMGNSYNINADYAAAAISGVLKAEKLLIMTDIDGVYTDITDPTTLL
                     SSITVAQTKEFISKGMIYGGMIPKLECCVAAINKGAKNVHLVDGRKKHGLLLNIIKDSG
                     TKIITAKGVI"
     misc_feature    complement(2930..2943)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: medium, score: 18.28"
                     /tool="antismash"
     gene            complement(3048..3461)
                     /locus_tag="LL065_RS17465"
                     /old_locus_tag="LL065_17445"
     CDS             complement(3048..3461)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678196.1"
                     /locus_tag="LL065_RS17465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17445"
                     /product="hypothetical protein"
                     /protein_id="WP_226135327.1"
                     /transl_table=11
                     /translation="MYVEFNNTIVESKDIKDTIEKNTEFKVLKDMSKGSKREDILAFNL
                     SVAINTLNDLLEDENNLKDLTEDELFEEYLSLAEEIAMDIEEYVPDGAVLDIKAYKWDK
                     SDDGISLVIIIAPEDINDLKLKDIMRKLITQVE"
     misc_feature    complement(3479..3494)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.13"
                     /tool="antismash"
     gene            complement(3617..6583)
                     /locus_tag="LL065_RS17470"
                     /old_locus_tag="LL065_17450"
     CDS             complement(3617..6583)
                     /EC_number="3.6.4.-"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968315.1"
                     /locus_tag="LL065_RS17470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17450"
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_264174711.1"
                     /transl_table=11
                     /translation="MELQSIIFKSSSESMRGQGKKAFNEGLVSKIKGKKINNMYHIYGK
                     VNNDTKSKELNTHITIDLTKMRLDSIKCTCEDFKEVSIVGHSFMCSHLTATAYSFLNIY
                     TKGNSEKSERDEKSEKISENDTSKKHISNIIKLNRKVAKGSIYYEAQHQIGMEKTKIEA
                     NYLRNFLEGLNYKKVKLNYDGFEFEAPIVKQDLPLTFTLKMREKHFVLTTHKQFPISLN
                     DKNDVYLLNWKLYLPSKDQVQRYILICERLRKNREILYGVNITNYNKIISFLGTISSDV
                     NISEEVKSFATNFIKLEFYLYEVDSHIYCDLLVVYGNEKINILKNDRSKDKMLREHKTN
                     TRDYKKEDKILMELEKYRFIKRNERLLFIGSDEQLFDILNKSKNNLYSLGNVTFGNGLK
                     HMKIQNAKNIKAEIKEMDDYYDFSYSIDNIYDNELKAVIDSFKQNNRFYKTKNNNFLNF
                     EDETVKSFFDLILVLSTGKLLENGSIKIEKNKALYLQNKIKNKKFEFIKGREVLKDIEN
                     IYDNINKMDIKVPVNLKATLREYQIDGFKWLKSLSTLGLGGILADEMGLGKTVQTIAFL
                     LSFENKKSIIISPTSLIYNWKAELEKFAPSLKVAIVHGEKKGRYELIHNLENYDIILTT
                     YGTLKMDLKQYDNIVFDYCIIDEAQNIKNYLAQCTKVIKEVKAKVRYALTGTPIENNLL
                     ELWSIFDFVMPNYLYSKETFELKFISGRKVDLGELKSLIKPFLLRRTKDEVLRELPDKI
                     EKKFIVEMTTSQKAVYKAYIKAVRDKMKNNNDGKIEVFSYLTRLRQICLDPSLVIEGYK
                     GGSGKINIATELIQKHVEDGGKILLFSQFTSALKNIGENLKEKGIPYYYLDGKTKSKDR
                     IKMVDEFNKSYQTFVFLISLKAGGTGLNLTSANLVIHFDPWWNPAVENQATDRAHRIGQ
                     KNVVEVIKLVAKGTIEEKIILLQENKKDLIDSVITGELTNSDVLGKLTKEELLEILMI"
     misc_feature    6699..6710
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.71"
                     /tool="antismash"
     misc_feature    6734..6749
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.58"
                     /tool="antismash"
     misc_feature    complement(6813..6827)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: medium, score: 23.67"
                     /tool="antismash"
     misc_feature    6814..6828
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 21.7"
                     /tool="antismash"
     gene            6984..7982
                     /locus_tag="LL065_RS17475"
                     /old_locus_tag="LL065_17455"
     CDS             6984..7982
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /locus_tag="LL065_RS17475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17455"
                     /product="3D domain-containing protein"
                     /protein_id="WP_169829570.1"
                     /transl_table=11
                     /translation="MAIITTSTIFVCSIRKNITVVIDGKSTNMVTYQKTFGNALKKSGI
                     SIDVKDKVDKALNSKIQNNGVITITRAINVNVFVDNKKLNVKSAEKDVALMLKAQQIDI
                     SPTDKISPSKETKLSSGLGITVTRVNSKTIKQSKPVDFKTVIKENDSILKSERTVSQNG
                     VNGLKSITLNVTYENGKEVTRKVVKETLVTQPKDKIIVQGTMPAVSVSRGSSISRESQV
                     AKATPISSKSNSYSKTFSVKTTAYWGSEGTNNTYTASGQKTVRNPNGYSTVAVDPSVIP
                     LGTKLYIEGYGNAIAADTGSAIKGKFIDLFFNNEGECGDWGVRYLTVHILN"
     gene            8223..9221
                     /locus_tag="LL065_RS17480"
                     /old_locus_tag="LL065_17460"
     CDS             8223..9221
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722449.1"
                     /locus_tag="LL065_RS17480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17460"
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="WP_226135331.1"
                     /transl_table=11
                     /translation="MKKIGLTTTVPVEILIAAGYTPVDLNNIFITSDDYLKYIDIAERD
                     GFPRSLCTWIKGIYGACLQNDIKEIVGVVEGDCSNTKALIEVLELRGIKVYPFSFPPSH
                     KLEDVEHEIKKFMNVFNVRIKEVEIVKNRLNRTRALVKEIDELTHIHNKATGFENHLYQ
                     VSSSDFNCNVDAFDVDLEKIIYEIKNRKPVIRKLRLGYIGVPPMTPDIYSFVENHNASF
                     VYNEVQREFAFPRTNNAMDIYHQYYDYTYPYDTNFRIIEIQKQIDNRKLDGIIHYTQAF
                     CYRAVADIVLKEKLNIPILNIEGDKLNVLDARTKLRLEAFLDMLLDLKEAK"
     gene            9221..9979
                     /locus_tag="LL065_RS17485"
                     /old_locus_tag="LL065_17465"
     CDS             9221..9979
                     /GO_function="GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722450.1"
                     /locus_tag="LL065_RS17485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17465"
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="WP_226135333.1"
                     /transl_table=11
                     /translation="MRVLGIDLGSREVKIVLMEDNKIIQKKKVSTMSFYRNYCNFDGKI
                     IVDLEKLDISHIDKAVSTGYGKNNTDLLLFSPINEIKAHVYGGFYQTGLNDFILLDIGG
                     QDVKIVKVEKGITTDLELNEKCAASCGRYLENMANVLEVSLGEMSKYYENAIELNSTCA
                     VFSESELIGKIAEGVSIESLCSSVNYSLYKRLLPLLSKFRGKKLILTGGVASNIAIKKY
                     LSNNYDEIVSIEDPQFNGAIGCCYYGSKFL"
     gene            10001..10432
                     /gene="queD"
                     /locus_tag="LL065_RS17490"
                     /old_locus_tag="LL065_17470"
     CDS             10001..10432
                     /EC_number="4.1.2.50"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="queD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     deazapurine: PTPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486363.1"
                     /locus_tag="LL065_RS17490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17470"
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="WP_226135335.1"
                     /sec_met_domain="PTPS (E-value: 8.2e-34, bitscore: 114.3,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYILKSEHSFDSAHFLSGYEGKCANIHGHRWKVEVEVQSETLTKG
                     GQLEGMVIDFGDLKRDVKSMADSYDHALIVQIGSMRDETLNCINQDGFKVILVDFRTTA
                     ENFAKFFFKQMKNKGYNVKRTTVYETPNNSASYEESEAH"
     gene            10434..11099
                     /gene="queE"
                     /locus_tag="LL065_RS17495"
                     /old_locus_tag="LL065_17475"
     CDS             10434..11099
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="queE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     deazapurine: QueE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966888.1"
                     /locus_tag="LL065_RS17495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17475"
                     /product="putative 7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="WP_226135336.1"
                     /sec_met_domain="QueE (E-value: 1.4e-41, bitscore: 140.6,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 5.1e-13, bitscore: 47.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.7e-15, bitscore: 55.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYKVVEKFISVNGEGPLSGQLVVFIRFAGCNLNCSYCDTTWANE
                     KDASYNLMTPDDIYEYIKSTQIRNVTLTGGEPLLQEGIIDLLNVLCFDDSIHIEIETNG
                     SINLGKFSNIENPPSFNMDYKLPSSNMEGPMDLNNFAYLTYKDTVKFVASSMEDLQKSK
                     RIIDKYALTNKTNVYISPVFGQIVLEDIVEFMKSNKMNGVNLQVQLHKIIWDPNKRGV"
     misc_feature    complement(10457..10472)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.05"
                     /tool="antismash"
     gene            11104..11694
                     /gene="folE"
                     /locus_tag="LL065_RS17500"
                     /old_locus_tag="LL065_17480"
     CDS             11104..11694
                     /EC_number="3.5.4.16"
                     /GO_function="GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="folE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     deazapurine: GTP_cyclohydroI"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004451708.1"
                     /locus_tag="LL065_RS17500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17480"
                     /product="GTP cyclohydrolase I"
                     /protein_id="WP_220723702.1"
                     /sec_met_domain="GTP_cyclohydroI (E-value: 4.1e-64,
                     bitscore: 213.4, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDTEAIEKHIRGILIALGDDPHREGLKDTPKRVAKMYEEVFAG
                     MCYSNAEIAKMFDTTFVDDLIVNDDSRDIVIMKDIQIFSHCEHHLALMYDMKVTVAYVP
                     NKKIIGLSKIARIADMVGRRLQLQERIGTDIAEIMQIVTNSADIAVIIEGEHACMTTRG
                     IKKPGTKTMSTTFRGRFNTDVILNNKLMMLYNK"
     gene            11731..12399
                     /gene="queC"
                     /locus_tag="LL065_RS17505"
                     /old_locus_tag="LL065_17485"
     CDS             11731..12399
                     /EC_number="6.3.4.20"
                     /GO_process="GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="queC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     deazapurine: QueC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061434.1"
                     /locus_tag="LL065_RS17505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17485"
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="WP_226135338.1"
                     /sec_met_domain="QueC (E-value: 1.3e-83, bitscore: 277.7,
                     seeds: 27, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNREKAVVVFSGGQDSTTCLFWALKKYKEVIAVSFDYGQKHILEL
                     DCAKTICSEHDVEFHVLDLNLLNQLAPNSLTRKDMKVDTSAPKDSVPNSFVDGRNLLFL
                     TFVAVFAKQRGISTIITGVSQSDFSGYPDCRDVFIKSLNVTLNLAMGYDFVIETPLMWI
                     NKAETWKMAYDLGVLDVVKEQTLTCYNGIKGNGCGDCPSCKLRKKGYIEFKEKYLIQKK
                     "
     gene            complement(12412..12990)
                     /locus_tag="LL065_RS17510"
                     /old_locus_tag="LL065_17490"
     CDS             complement(12412..12990)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966650.1"
                     /locus_tag="LL065_RS17510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17490"
                     /product="hypothetical protein"
                     /protein_id="WP_226135340.1"
                     /sec_met_domain="HAD_2 (E-value: 0.0042, bitscore: 15.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLNICIDIDGTITDAYYWLNITNKYFNKNITEDEVTQYYIHEI
                     MGVTLKEYDEFYQENKFEIHSEQILREDVQIVIKKLSELHNIYFVTAREKELTMFTHSY
                     LKRNEILYDELFVLGSHYKVNKARELECNVFIEDNYDNAIQLSNAGFKVLLIDTNYNRK
                     SLNDNIVRVYNWKEIYYIINKLLLQSKAM"
     gene            complement(13095..14036)
                     /locus_tag="LL065_RS17515"
                     /old_locus_tag="LL065_17495"
     CDS             complement(13095..14036)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /locus_tag="LL065_RS17515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17495"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_226135342.1"
                     /transl_table=11
                     /translation="MDSYNNTICMKLKLDGEDNKAVRTLCAICYEKQKTYLKLELDLKK
                     SQRKENKKDKALLEFLYYENKMLVGYLGVCNFGGDSVQISGMVHPDFRRKGIFRKLYLM
                     AKEEWQRISKAEILALCDHTSTSGIEFINSIGGEYVSSEYKMCLTKKALDGESIHKQEI
                     DSLIKLRLATSEDAAEIEKQTSIYFGKPLNEIEDKEAKVVNMDKEDLENSFIQQGNNFI
                     SYMALLKEEIIGKVHISIINKEGFIYGFGVLPVFRGKGYGREVLLATLDILKEKGVDNI
                     FLEVESQNKNALGLYESCGFEEVAVMDYYVIT"
     gene            complement(14064..14744)
                     /locus_tag="LL065_RS17520"
                     /old_locus_tag="LL065_17500"
     CDS             complement(14064..14744)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS17520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_17500"
                     /product="hypothetical protein"
                     /protein_id="WP_226135344.1"
                     /transl_table=11
                     /translation="MEKYVYVIISRTPTSTGKIVRKFLKEKYNHASISLDKNLSQMYSF
                     CRFSVSNPLVGGIVRESAFTLTIGLKENVPIKIYRIPVTAEKYELISKFIYGVYNDTEV
                     YYYNFLQAIGLINNKRHAIYKTYICTEFVMEALRQAGISLTTLEPYQITPTDICRIMGE
                     FICYSGNLDDYPFRIQIKTKNDELFFCKTGFFYEGLHTIKHFWMVVSRDRTSKRVSKSK
                     RSRI"
     gene            15147..17234
                     /locus_tag="LL065_RS17525"
                     /old_locus_tag="LL065_17505"
     CDS             15147..17234
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /locus_tag="LL065_RS17525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17505"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_226135346.1"
                     /transl_table=11
                     /translation="MRKEYISYMNDLPISISLKNINDYPIHWHNSIEIIFVLEGSINVL
                     IESGNYEVYERELEIINCDEAHRIYSEVENKVLIFHLDPIFFEKYYDDMKNIYFYTNSS
                     EEGAQREEKYDVLRKYLSILACEVIQKSENFDDQIESILVKLLFHLINNFHYLTYDEKD
                     IKENVTQFQRYHRIIRYIYNNYMNKISLQDIANIEYLSSYYLCHQIKEMSGTNLKELVN
                     NVRVDESIKLLLDTDKTISDISFDVGFSHARYYNKSFKDHYKCTPLQFRKKYDIPADKF
                     DSLKKIQTYDLSTSIQYLSNYLEDYDRFNYTNKIIKLQTDFTLKGTQLYHKWEENINLG
                     QAKELIKAREQEYLRTFQEAIGFKNGIVQNLFGKEMKIYFNENVNFLNWNEVEKLLEFL
                     MDISLVPVIILDQIFEDKSSFIKLLESFILYFADIYGIEVVKLWKFQVSKDLSIEYIDI
                     SKQIFEKYLLQDLIEEPFNGPEAINTIYDSSYMLPYIIHNFINDTSSLNALKAFDTVEE
                     NSILNNELFFGSPGLLTLDGIKKASYYAYYLLSKLGNEVIDKGDGYIITRRDEDIQVLL
                     YSYSDELNTLIKLEDLAKGKGRKNITERKISLSIKNLIHDYKVIKYEIGEKVGSAYDIW
                     LRMGKPKRLGYDEWKLLTKISTPNISLSYAKKNAVYNNIIKIEGYGSTLIVLQEVQKHL
                     F"
     gene            17382..18809
                     /locus_tag="LL065_RS17530"
                     /old_locus_tag="LL065_17510"
     CDS             17382..18809
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /locus_tag="LL065_RS17530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17510"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_226135348.1"
                     /transl_table=11
                     /translation="MTISNITESNKMFGEEKIGKLLFKFSVPAIISLMVAEMYNMVDSM
                     FLGQAIGANAIGALTIAFPIQRLFFAISMLIAIGASTSVSRSCGDKDFENLKIIIPNAI
                     VLMFITISIIGISIFIFQDKILISLGASKNTFSLAKDYISIILIGVLFQSFTIIASYII
                     TSFGNTKIVLLSTSIGAIFNVIINYFLVNVFSYGVKGAAIATVVSQIIAFIYVLIVFIQ
                     LKMSLKFSFKLTLNKAVSVGIVCVGFSTFIIEISDAIVAAILNNLLFSYGGDSAIVTIG
                     LTTRVSMFLFMTVMGISTAMQPIAAYNFGAGNYTRLKEVVKSSIIAVSVSSTLLWAIFM
                     IFSDTFIGLFINDKAIIEQTSKAFRMVIAVFPCIGVYYLSISYCQAINRVKASFKLSIF
                     RQLIVFIPLVYSFVIGLNMGVSGAWLAYPISDVISFITAAIFIRYTYTSLEILERHQIF
                     KLNKIKIRLASIASTAS"
     gene            complement(18884..19534)
                     /locus_tag="LL065_RS17535"
                     /old_locus_tag="LL065_17515"
     CDS             complement(18884..19534)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /locus_tag="LL065_RS17535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17515"
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="WP_226135350.1"
                     /transl_table=11
                     /translation="MGALVLLSGFTKKDNVNLIKRMKEIFLDRKYTLSYIPSMTDKKLE
                     SFKKAKIQLREYGNFEFKYFDIDDFCSIDTIDAIFKSDVIYLSGGNAYYFLNNLKKRYF
                     ITRLRRYVQNGGCIIGVCAGAIMMAKDISAAQFGSEDIVGLSNLSSLALVDFNFMPHWN
                     MCSKYLDDLKKYSNNTGNVVYTCNDGDGIVVINNELQFYGDINMIKEGEIEQV"
     gene            19891..21546
                     /gene="gltX"
                     /locus_tag="LL065_RS17540"
                     /old_locus_tag="LL065_17520"
     CDS             19891..21546
                     /EC_number="6.1.1.17"
                     /GO_component="GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA]"
                     /GO_function="GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="gltX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299803.1"
                     /locus_tag="LL065_RS17540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_17520"
                     /product="glutamate--tRNA ligase"
                     /protein_id="WP_226135353.1"
                     /transl_table=11
                     /translation="MDYLKLADLLLPNVKETPEYYRTLYPKRNLKPNAMITRYAPSPTG
                     FQHLGGVFAALISERLAHQSEGIFYLRVEDTDKKREVIGAIEDSTNTLRDFGINFDEGV
                     IDSTSEKGAYGPYKQSDRINIYHAFVKSLIKKGLAYPCFCTEEDLDKTRTIQQELKINT
                     GYHGKWAVHRNSTMDEINSELNKGTPYVIRLRAPEASGKRVVCNDLIKGTISMPENDQD
                     IVILKTNGIPTYHFAHAIDDYLMGTTHVIRGEEWLSSLPIHLQLFEMLGFTPPQYAHIP
                     TIMKMDGASKRKLSKRKDPEISMNFYKSEGYPFTSVAEYLINLINSDFQEWRDTHANIP
                     CTSFNITLENMSKSGALFDILKLNDISKNVIATMNAATVYELYLNWAKDYDVKMSNLLI
                     ENEEYAKKIFNIERENDKPRKDFVKWLDVKEHIEYFFDDLYKDSLTKGYNFPDHIPMDE
                     VRSVLSSYIGVYDYSHDNDTWFATLKELALNLGYAKNAKTYKKNPELFKGQLSDVATIV
                     RVALTNKTQTQDLHEVMQAMGEKRVIERLSQDFK"
     misc_feature    21754..21768
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 18.44"
                     /tool="antismash"
ORIGIN
        1 ggtgaggcta aaagtggctt agaagctatt gaaatggcta aaacaagtac ccccgatatt
       61 attttaatgg atgtagaaat gcccgaactt agcggccttg aggctcaaaa agagataatt
      121 aaatttttac caaatgtaca cacaatcatt ctatctgctt atgatgattt tgcttttact
      181 cagagtgcaa ttagaataaa agtaatcgat tacctactga aacccataag acctaacgat
      241 ttaattgaat ctattaatag gagtatatct ttaataaata cgcaaaatga tagagtcata
      301 agcgataacc aagatgatac gctaattaaa gaagctatga attatatcga tgaacattat
      361 aatacatata taagtcttca atcaattgca gcttatgttc atcttaaccc acaatatttc
      421 agcagatact ttaaaagtaa aattggtatt aactttatta attatttatc ttcctttaga
      481 gtaataaaag caaaagactt gctcctaaat acagataaaa acattacgga aatatcacaa
      541 aatgttggat atgttgattc aagttatttc agtaaagttt ttttaaaaag tgttggaatg
      601 tcgcccaatc actttaggtg tcaaaacaag aaacaacatt tctaaagata aaagctgcat
      661 aacgaattcg ttatgcagct tttatcaatt tactttattt ttatagtaaa cttacacatt
      721 aaatttctga tattactctt tcaagaatat ctaatgcttc atcaatctct ccctttgtta
      781 cattaagtgg aggaagtaat cttaccacat tattcccggc cgctattaca agcaatttct
      841 tgtcaaaaca cgacttacaa aatttctttg tatctgtctt tatttttatg ccaaccaata
      901 gacctatacc ttttatttca tctattacac catatttttc ttttatttta agtaatcgtt
      961 ctttcatata tgcacttttg tcgtcaattt tttctataac tccaccatca agaagttctg
     1021 ttaaaacagc ctcaccaaca gcgcatgcaa gtggatttcc accaaatgtg cttccatgat
     1081 cacctggtac aaatgccgct gacgctttaa catttgtaat agttgcccct attggaaaac
     1141 cgccaccaag tgcttttgca atacatataa catcaggaat aactgaaaat ttcttataag
     1201 caaaaagact tccaaggcgt cccataccgc attgtacttc atcaaaaatt aatagagcat
     1261 catatttatc acacaaatct cttgctgctt ttaagaactc tattgttgca atatttattc
     1321 cgccttctcc ttgaatcggc tctactatta cagcgcatac tttatcatta aaattctttt
     1381 ttagctcttc tacatcatta aattctacct tttgtgtacc tccaattaat ggacggaaat
     1441 ctttttgata tttttcctga ccagtaactg ataatgaacc catagttcta ccatgaaaag
     1501 actcgtccat atatataagt acatttttat ctttagttgt ttttaatgct ccatattttc
     1561 tcgctatctt gagagctgct tctatggctt ctgtaccact gttacaaaaa aatactttat
     1621 cgtgatcaca gttatctatt agtttttttg caagtagctt atgcggtgta ttccaataat
     1681 aattagaact ctgcattagc ttactacttt gcgtagttat tgcatttatt atagcaggat
     1741 ggctatgtcc aagacaattc actgcaactc ctgatacaaa atccatatac tcttgaccca
     1801 ctgaatcata aattttacat ccaagacctt tctcaaaagt aacatcgtat cttccataag
     1861 tattcattac acaattttct tccattatat tacccctttc gctgttataa ttttagttcc
     1921 actatcctta attatattta atagtaaccc atgttttttt cttccatcta ctaaatgaac
     1981 attcttagct cccttattaa ttgcagcaac gcagcattct agttttggaa tcattccacc
     2041 ataaatcata cccttactta taaattcttt agtttgagca accgttattg atgataaaag
     2101 tgttgttgga tcagttatat ctgtatatac accatctata tctgtcataa ttaataactt
     2161 ttcagcttta agcactccac ttatagcagc tgcagcataa tccgcattta tattataact
     2221 atttcccatg caacctaccc caactggaga aataactggt atatacttat ttttaagtaa
     2281 cgtaagtagt acctttgtgt taatgtttgc aacctctccg acgaaaccta tatcaatttt
     2341 tctgccatct tcataaacat acttcttttt agattgaatc aagttactat ctctaccact
     2401 tataccaata gcatttagac ctctaagact taatattgat gataatcttt tattaacatt
     2461 acctgataat accatctcta caatttccat agtctcctta cttgtaactc ttaatccttc
     2521 tataaacttg ttttcaccac cgcttttctt tacccacttg ctaatcaaag gacctccacc
     2581 atgtacaata acaactttta tgcctgcttt tgtcataagc tcaatatctt ctacaaaagc
     2641 ttccttagca acatcattac tcattatact tccaccatat tttatcacaa aggtcttacc
     2701 ctcatattta ctcatttgca aaaaatcttt tttatttgat tcattattta atatatccat
     2761 tataacctcc aaacctaata cttatttttt actaattatt taagtatttt atcatttgaa
     2821 taattataca tcaatttgaa taattattca acatattgtt tatttttttt atgattttct
     2881 tatgtttatt aattaatagt acatttataa gcaattacac catttccatt gcataattat
     2941 gcaataaggc aacaaagtgc cacccaagtg gcaaatgtga caaactcata taaataaaaa
     3001 accataacaa gatttaaatt attttaatct tgctacagtc ctaattgtta ttccacttga
     3061 gttattagtt ttctcataat atcctttaat ttaagatcat ttatatcttc tggagctata
     3121 attataacaa gacttatgcc atcatctgat ttatcccatt tataagcttt gatgtctagt
     3181 actgcgccat ctggtacata ctcttcaatg tccattgcaa tttcttctgc aagtgataga
     3241 tattcttcaa ataattcatc ttctgttaaa tcctttaaat tattttcgtc ttccagtaaa
     3301 tcattcaagg tattaatagc tacacttaga ttaaaagcta atatatcttc tctctttgaa
     3361 cctttactca tatctttaag tactttaaat tctgtgtttt tctcgattgt atcttttata
     3421 tctttactct ccacaatcgt attattaaat tctacgtaca tattttcatc tcctattggt
     3481 gccacccacg tgacaagtgg catcttatac tattttatta cgggaaatca gtttacttat
     3541 agttgaaggt gctaaactta agtattctgc tatttcttgc tttattatat cataatcatt
     3601 gtcatatggc tggcagctaa atcatgagta tctccaaaag ttcttctttt gtaagtttcc
     3661 ctaaaacatc gctgttcgta agttcccctg taattacact atcaattaga tcttttttat
     3721 tttcttgaag tagaatgatc ttttcttcaa tagtcccttt tgcaactaat ttaattactt
     3781 caactacatt tttttggcca attctatgag ccctatctgt ggcttgattc tcaactgccg
     3841 gattccacca agggtcaaaa tgaattacca aatttgctga tgttaagttg aggcctgttc
     3901 cccctgcctt taatgatatt aaaaatacga atgtttgata acttttattg aattcatcta
     3961 ccatcttaat tctatcctta gatttagttt ttccatctaa ataataatat ggtattcctt
     4021 tttcttttaa gttttctcct atatttttaa gcgcagatgt aaattgagaa aacaatagta
     4081 tttttccacc gtcctccaca tgtttttgaa taagttctgt tgcaatgttt atctttccac
     4141 ttccgccttt atatccttct atcacaagtg atgggtctag acatatttgc ctgagccttg
     4201 ttaaataaga aaaaacctct attttcccat cattattgtt tttcatttta tccctgacag
     4261 ccttgatgta ggctttatat acagcctttt gagatgtggt catctcaact ataaatttct
     4321 tttctatttt atctggcaat tccctaagca cttcatcttt agttcttctt aataaaaaag
     4381 gtttaataag tgattttagt tctcctaaat caacctttct tccagaaata aatttaagtt
     4441 caaaagtttc tttggaatat aaatagtttg gcattacaaa atcaaaaata gaccaaagtt
     4501 ctaataaatt attttcaata ggagttccag ttaaggcata tcttacttta gctttaactt
     4561 ctttaataac ttttgtgcac tgcgccaaat agttttttat attttgcgcc tcatctataa
     4621 tacaataatc aaaaacaata ttatcatatt gttttaagtc catttttaaa gttccatagg
     4681 tcgtaagtat tatatcataa ttctctaaat tatgaattaa ctcatacctt cctttttttt
     4741 ccccatgtac aattgcaacc tttaggctag gagcaaattt ttctagttcc gccttccaat
     4801 tataaataag ggaggttgga ctaatgataa tagatttttt attttcaaag gataagagaa
     4861 aggctatggt ctgaacagtt tttccaaggc ccatttcatc tgctagtatc cccccaagtc
     4921 ccaaagtgct aaggctttta agccatttaa aaccatcaat ttgatattcg cgaagcgtag
     4981 cctttaagtt cacaggtacc tttatgtcca ttttatttat gttatcatag atgttttcta
     5041 tatcctttag cacttcgcga cccttaataa attcgaattt cttatttttt attttgtttt
     5101 gtaaatataa cgccttattt ttttctatct ttattgagcc attttctagt aacttaccag
     5161 tgcttaatac taatattaga tcaaaaaagc tcttaaccgt ctcatcttca aagttcaaaa
     5221 aattattgtt cttagtttta taaaatctat tattttgttt aaatgaatca atcacagctt
     5281 ttaattcatt atcgtaaata ttgtctatac tataagaaaa atcatagtaa tcatccattt
     5341 ccttaatctc tgcttttata ttctttgcat tttgtatttt catatgctta agtccatttc
     5401 caaaagtaac atttccaagg gaataaagat tgtttttact cttatttaat atatcaaaca
     5461 gctgctcatc tgaccctata aaaagtagcc tctcatttct ttttataaac ctatatttct
     5521 ccagttccat aaggattttg tcttcctttt tgtaatcacg tgtatttgtt ttatgctcac
     5581 ggagcatttt atctttactc ctatcattct ttagtatgtt aattttttca ttaccataaa
     5641 ctactaacaa gtcacaataa atatggctat ctacctcata caagtaaaat tctaatttta
     5701 taaagtttgt tgcaaaactt ttgacttctt cactaatatt tacatcactt gaaatagtac
     5761 ctaaaaaaga tatgatttta ttataattag taatatttac gccatataaa atttcacgat
     5821 tttttcttaa cctttcacaa ataagaatat acctttgtac ttgatccttt gatggtaagt
     5881 acagtttcca attgagtaaa tatacatcat ttttgtcatt taatgatatt gggaattgct
     5941 tatgcgtagt taaaacaaaa tgcttttctc gcatctttaa cgtaaacgtt agaggtagat
     6001 cctgctttac aattggtgct tcaaactcaa acccatcata atttaattta acttttttat
     6061 aattaagacc ttccagaaaa ttccttaagt aatttgcctc aatttttgtc ttttccatac
     6121 ctatttgatg ttgagcctca taataaattg aacctttcgc tacttttcga tttaacttta
     6181 taatattact tatgtgcttc ttactagtat cattctcact tatcttctct gatttttcat
     6241 ctctctctga tttttcagaa ttaccctttg tatatatatt caaaaaacta tatgctgtag
     6301 ctgttaaatg actacacata aatgaatgac ctacaataga gacttctttg aaatcctcac
     6361 aggtacattt tatactatct aatctcatct tagttaaatc tattgttata tgtgtgttta
     6421 attcttttga tttagtatca ttattaacct ttccataaat atgatacata ttatttatct
     6481 tttttccttt gattttacta actaatccct cattaaaagc cttcttaccc tgacctctca
     6541 tactctctga agatgatttg aatattatac tttgtaactc cattaacttc acgtcttaat
     6601 cacctccaaa ttaataatac ccctaaaatc acataaatta aacgaactaa gcttttatat
     6661 gctataataa ttatatcact taagggaaag ttgggtaaga aatcgttatc atttgcatct
     6721 ttttaataaa cgtgttacaa tattgttaca ataatttacc aatacattta ttggtataag
     6781 tatacatgac tcccctactt ctcatgcatt ttatgagagc gctcttaatt gtaatatacc
     6841 cgagagcact gtagacctaa gtggttaggc atctgtaata ataaatccat gatgccttat
     6901 actgaaatta ataataggag atgattttta tatttaaatt tatggaaggt aaacgaataa
     6961 aatttgtaat tggattcata ctggtggcaa taattacaac ttcaactatt tttgtgtgct
     7021 ccattagaaa aaacattacg gtagttatag acggtaaatc aactaatatg gtaacttatc
     7081 aaaagacatt tggtaacgcc ctaaaaaaat ctggtataag catagacgta aaagataaag
     7141 ttgataaggc tttgaattca aaaatacaaa acaacggtgt tataactata acccgtgcta
     7201 taaacgttaa cgtctttgtg gataataaga aacttaatgt taaatctgct gaaaaagatg
     7261 ttgctttaat gttaaaggca caacaaatag acataagccc tactgataaa atttcaccat
     7321 caaaagaaac taaattatcg agtggcctag gcataacagt aactagggtt aattcaaaaa
     7381 caataaaaca gtcaaaacct gttgatttta aaactgttat taaagaaaat gatagtatat
     7441 taaaatctga aagaacagtt tcacaaaatg gagttaatgg cttaaaaagc attacattaa
     7501 atgtaactta tgaaaacgga aaagaagtaa ctaggaaagt tgttaaagaa accttagtta
     7561 cacagcctaa agataaaatt atagttcaag gtactatgcc agcagtatca gtttcaaggg
     7621 gatcttcaat ttccagagaa tctcaagttg caaaagcaac acccatttct tccaaaagta
     7681 attcatatag taaaactttt tctgtaaaaa ctactgctta ctggggttct gaaggaacta
     7741 ataacactta tactgcttca ggacaaaaga ctgttagaaa tccaaatgga tatagtaccg
     7801 tagctgtaga tccaagtgtc ataccgcttg gaacaaaatt atatatagag ggttatggaa
     7861 atgctatcgc ggctgatacc ggttcagcaa taaaaggaaa atttatagat ttattcttta
     7921 ataacgaagg tgaatgcggt gattggggtg tacgatacct gacggtgcat atactaaact
     7981 aaatatttta tatacttaat tatacaaaaa ctgctacaga tattatttct gtggcagttt
     8041 ttatttttca agtcaatttg acatagcaat taaaatatag taagctttat taatataaaa
     8101 cttaaataag acagttctaa accatcctgt ctataaacaa aactaggacg taactaccgt
     8161 tttaggtagt ttttttgttt ggtatcagat ggttcttttg ttatatttta ggaggaatca
     8221 taatgaaaaa aataggttta acaactactg ttccagtaga aattctaatt gcagcaggtt
     8281 atacacctgt cgatttaaac aatattttta taacctcaga tgactatttg aaatatatag
     8341 acattgctga aagagatggc ttccccagga gtttatgtac ctggataaag ggtatttacg
     8401 gagcctgttt acaaaatgac ataaaagaaa tagttggagt tgttgagggc gattgttcta
     8461 acacaaaagc tctaattgaa gtactagaac tacgaggaat aaaagtttat ccattttcct
     8521 tccctcctag tcacaagtta gaagatgtag aacatgaaat aaaaaaattt atgaatgtct
     8581 ttaatgtaag aataaaagaa gttgaaatag ttaagaatcg cctgaatagg actcgcgcct
     8641 tggtcaaaga aattgatgaa ttaacccata tacataataa agcaacaggc tttgaaaatc
     8701 atctttatca agtaagttca agtgatttta attgtaatgt agatgctttt gacgttgact
     8761 tagaaaaaat catttatgaa attaaaaaca gaaaaccagt aattagaaaa cttcgccttg
     8821 gctatattgg tgtaccccct atgactccag atatctactc atttgttgaa aatcataatg
     8881 ctagttttgt atacaatgag gttcaaagag aatttgcttt tccacgaact aataatgcca
     8941 tggatatata tcatcaatat tatgattata cttatcctta tgatacgaat tttaggatta
     9001 tagagattca aaaacaaata gacaatcgga aattagacgg aataattcac tacactcaag
     9061 cattttgcta tagggcagtt gcagatatag ttttaaaaga gaaattaaat attcccatat
     9121 taaatattga aggcgacaaa ttaaacgtct tagacgcaag aacaaagctc aggttagaag
     9181 ccttccttga tatgttacta gatttaaagg aggctaaata atgcgcgtac tgggtataga
     9241 ccttggaagc cgcgaggtta aaatagtttt aatggaagac aataagataa tacaaaaaaa
     9301 gaaagtaagc acaatgagtt tttacagaaa ttattgtaac tttgatggta aaattattgt
     9361 tgatttagaa aagcttgata ttagccatat tgataaagca gtttctacag gttatggtaa
     9421 aaacaacact gatttattac tattctcacc aataaatgag ataaaagctc atgtatatgg
     9481 aggattttat caaacaggtt taaatgattt tatactttta gatataggtg gtcaagatgt
     9541 taaaattgta aaagtagaaa aaggtatcac aacagaccta gaacttaatg aaaaatgcgc
     9601 tgcatcttgt ggtagatatt tagaaaatat ggcaaatgtg ctagaagtat cacttggcga
     9661 gatgagtaag tattacgaaa atgcaataga acttaattca acttgcgcag tattttctga
     9721 atcagagtta attggtaaaa tagcagaagg tgtaagtata gaaagtttgt gctctagcgt
     9781 taattactcc ctatacaaaa ggctcctacc attacttagt aaatttcgcg gtaaaaaatt
     9841 aatcttaaca ggtggtgttg caagtaatat tgcaatcaaa aaatatttaa gtaacaatta
     9901 cgacgaaata gtatctatcg aagaccctca atttaatggg gccattggct gttgttatta
     9961 cgggagtaaa tttttataaa ttttaggagg aatgccccat atgtatattt tgaaatcaga
    10021 acatagcttt gatagtgcgc attttctttc tggatatgaa ggaaaatgtg caaatattca
    10081 tggacataga tggaaggttg aagtcgaagt acaatcggaa accttaacaa aaggcggtca
    10141 attagaaggt atggtaatag attttggtga tttaaaaagg gatgttaagt ccatggctga
    10201 ttcctatgac catgctctaa ttgtgcaaat aggaagcatg agagatgaga ccctaaattg
    10261 tataaatcag gacggattta aagtaatact tgttgatttt agaacaacag ctgaaaactt
    10321 tgcaaaattt ttctttaaac aaatgaaaaa taaaggatat aatgtaaagc gaactaccgt
    10381 ttatgaaaca cctaataata gcgcatcata tgaagaaagc gaggcacatt aaagtggaat
    10441 ataaagtagt tgaaaagttt ataagtgtta acggcgaagg tccactatca ggtcaacttg
    10501 tagtatttat aagattcgca ggttgtaacc ttaattgtag ctactgtgat accacttggg
    10561 ctaatgagaa agatgccagt tataacctta tgacacccga tgatatatat gaatatataa
    10621 aatctaccca aattcgaaat gtaaccttaa ctggtggcga acctctatta caagagggaa
    10681 ttatagatct cttaaatgtt ttgtgtttcg atgactcaat tcatattgaa attgagacta
    10741 atggtagtat taatttaggt aagttttcga atatagaaaa tccaccaagc tttaatatgg
    10801 attataaatt accatcaagc aatatggaag gtccaatgga tttaaataac tttgcttatt
    10861 taacttataa agatacagtt aaatttgtgg ctagtagtat ggaagattta caaaaatcca
    10921 aacgtataat agataaatat gcactaacaa ataaaacaaa tgtatatatt agtccagtat
    10981 ttggacaaat tgtcctagaa gacattgttg aatttatgaa aagcaataaa atgaatggtg
    11041 ttaatcttca agttcaactt cacaaaatca tttgggatcc aaataagaga ggtgtataag
    11101 attatggcaa ttgatacaga agcaattgag aaacatataa gaggcatttt aatagcacta
    11161 ggtgatgacc cccaccgcga aggcctcaaa gataccccta agcgtgtagc taaaatgtac
    11221 gaagaagtat ttgccggtat gtgttatagc aatgctgaaa ttgctaaaat gtttgataca
    11281 acatttgttg atgatttaat agttaatgat gattctcgtg atatagttat aatgaaagat
    11341 atacaaattt ttagtcattg tgaacaccat ttggcgctta tgtatgatat gaaagtaaca
    11401 gtagcatatg ttcctaataa aaagattatc ggtctaagta aaatagcaag aatagctgat
    11461 atggtaggcc gaagacttca acttcaagaa agaataggaa cagatattgc agaaattatg
    11521 caaatagtaa caaactctgc ggacatcgct gttattatag aaggtgaaca tgcctgcatg
    11581 actactcgtg gaataaaaaa accaggtact aaaacaatgt ctactacttt tagaggaaga
    11641 tttaatactg acgtaatttt aaacaataaa ttaatgatgc tttataataa ataagtaaaa
    11701 ttcaaacaaa ttaaaaggag cgtgtagatt atgaatagag aaaaagcagt agtagtattc
    11761 agtggcggac aagatagcac aacttgttta ttttgggcat taaaaaagta taaggaagtt
    11821 atagctgtgt cttttgatta tggtcaaaaa catatattag aacttgattg tgcaaaaact
    11881 atatgcagcg agcatgacgt tgaatttcat gttttagatt taaatttact taatcagtta
    11941 gcaccgaatt ccttaacaag aaaagatatg aaagttgaca cgtcagcccc aaaagatagt
    12001 gttcctaatt cctttgtaga tggacgtaac ttattatttt taacatttgt agccgtattt
    12061 gctaagcaaa gaggtataag tactattata accggtgtat ctcaaagtga ttttagtgga
    12121 tacccagatt gcagagatgt gtttattaaa tccttaaatg ttaccttaaa tttagctatg
    12181 ggttatgatt ttgtaattga aacacctttg atgtggatta acaaagcaga aacttggaaa
    12241 atggcttatg atttaggtgt gcttgatgta gttaaagagc aaacattaac ttgttataat
    12301 ggtataaaag gtaatggttg tggagactgc ccttcttgta aattaagaaa aaagggttat
    12361 atagaattca aagaaaaata tttaatacaa aaaaaataaa acaaactgtt attacattgc
    12421 tttgctttgt aataacagtt tgtttattat ataatatatt tctttccagt tataaaccct
    12481 aactatattg tcatttaatg atttcctatt ataattcgta tctattaaaa gaactttaaa
    12541 tcctgcattt gatagttgaa ttgcattatc gtaattatct tcgataaaca cattgcattc
    12601 taactccctt gctttattca ccttataatg actacctaaa acaaacaact catcatatag
    12661 aatttcattc ctctttaaat atgaatgagt aaacatagtg agttcttttt ctcttgctgt
    12721 aacaaaataa atattatgaa gttcacttag tttttttata acaatttgaa catcttctcg
    12781 aagtatctgc tctgaatgga tttcaaattt gttttcctga taaaattcat catattcttt
    12841 taaagtaaca cccattatct catgtatata atactgggtt acctcatcct ctgttatatt
    12901 tttattgaaa tatttattag taatattaag ccaataataa gcatctgtta ttgttccgtc
    12961 aatatcgata catatattta aatttttcat cttaccttca ctctcctctc tatgctttca
    13021 ttatatgcat gaaagattaa gaacaggtta aataaaggtt atgttctatt taaataatat
    13081 tatcaccaat cgctttaagt tattacataa taatccatta cagcaacctc ttcaaaccca
    13141 caagattcgt aaagtccaag tgcattttta ttttgcgact caacttcgag aaaaatattg
    13201 tcaacgcctt tttcttttaa tatatctaat gtagctaata gaacttcacg tccataaccc
    13261 tttcctctaa agactggaag cactccaaat ccataaataa agccttcctt attaattata
    13321 ctaatatgaa ccttccctat aatttcctct ttaagaagcg ccatatagct aatgaaatta
    13381 ttaccttgct gaatgaagga gttttctaag tcttctttat ccatattaac tactttagct
    13441 tctttgtcct ctatttcatt taatggtttt ccaaagtaaa tagaagtttg tttttcaatc
    13501 tcagctgcat cctcactagt tgcgagtcta agtttaataa gactatcaat ctcttgttta
    13561 tgtatacttt ccccatccaa agctttttta gttaaacaca ttttatactc tgaggataca
    13621 tattcacctc caatagaatt tataaattct attccagaag tagatgtatg atcacatagc
    13681 gctagaatct cagctttcga tattctttgc cattcttcct ttgccataag atataatttt
    13741 ctaaatatcc cctttcgcct aaagtcagga tgtaccatac cgcttatttg gactgaatct
    13801 cctccgaaat tgcatactcc taaatatcct actaacatct tattttcata gtaaagaaat
    13861 tctagcagag ctttgtcttt tttattttct tttctttgac tcttttttaa atcaagttct
    13921 aattttaagt aggtcttttg tttttcataa caaatagcgc aaagggtcct aacagcctta
    13981 ttgtcttccc catctaattt taatttcata caaatagtat tattataact atccatcatt
    14041 ctccccctag tatttacctt aaattaaatt ctacttctct tgcttttact aactctttta
    14101 ctagttctat ctctagaaac aaccatccag aagtgtttaa tagtatgtaa tccttcataa
    14161 aaaaaacctg ttttacagaa aaaaagctca tcatttttcg tttttatctg tatcctaaat
    14221 ggatagtcgt ctagattacc tgaatagcaa ataaattcac ccatgatcct gcaaatatct
    14281 gtaggtgtta tctgatatgg ttctaatgta gtaagactta ttccagcttg ccttagagct
    14341 tccataacaa attcagtgca tatatatgtc ttgtaaatag catgcctttt attattaata
    14401 agcccaatag cttgaagaaa attatagtag taaacttctg tatcgttata aaccccataa
    14461 ataaatttac ttatcaattc atatttttct gcagttacag gtatccgata tatttttata
    14521 ggtacattct cctttaatcc tatagtaagg gtaaacgcac tctcacggac tatgcccccc
    14581 acaaggggat tacttactga gaatctacaa aaggaataca tttgacttaa atttttatct
    14641 aagctaattg aagcatgatt gtatttttct tttaaaaatt ttctaactat ctttcctgta
    14701 gatgttggtg ttcttgaaat aataacataa acatactttt ccatttcatt tctccttgta
    14761 tccacttata taaataggtg actttccttc taaaagatgt ctaaacttta tattttcata
    14821 taatattatt tttagaatta aaaaaatgaa attatttata ttataaactt tagcatactt
    14881 attttccaca aaaatcatct ttatataata tatctttatc agttactata ttctaataac
    14941 attattatta tattgttccc aattacccaa tttttatgtt atttataatt aactaagttt
    15001 ttattgacta ataaaattaa ccattatgtt tcattattac ctaaaatata aaatttaaat
    15061 tacataataa attatgtagt gtatcatctt gtaatttaag atgtataata tatatacaca
    15121 taatcgcaag taaagaggtt actacaatga gaaaagaata tataagttat atgaatgatt
    15181 tacctataag tatctccctt aagaatataa atgactaccc aatacattgg cataattcta
    15241 tcgaaataat ttttgtacta gagggcagta ttaatgtact tatagaatca ggcaattatg
    15301 aagtatacga gcgtgaactt gaaattataa attgtgatga agcccacaga atttactcag
    15361 aggttgaaaa caaggtatta attttccact tagaccctat atttttcgaa aaatattatg
    15421 atgatatgaa aaatatatat ttttatacaa attcaagtga agaaggcgca caaagagaag
    15481 aaaaatatga tgttctacga aaatatttgt ctattttagc ttgcgaagta atacaaaaaa
    15541 gtgagaattt cgatgatcaa atagaatcca ttttagtaaa gttactattc catttaataa
    15601 acaattttca ttatcttaca tatgatgaaa aagatataaa ggaaaatgtg acgcaatttc
    15661 aaaggtatca tagaatcatt aggtatatat acaataacta tatgaataag ataagtttgc
    15721 aagacatagc aaatatagaa tatttaagtt cttattattt atgtcatcaa ataaaagaaa
    15781 tgtctggaac aaatttaaaa gaattagtta ataatgtacg agtagatgaa tctattaaat
    15841 tgctattaga tactgataaa accatatccg atatttcttt tgatgttggt ttctctcatg
    15901 ctaggtatta taataaaagc tttaaagatc attataagtg cacaccacta caattcagaa
    15961 aaaaatatga tataccagca gataaatttg atagtttaaa aaaaatacaa acttatgacc
    16021 tatctacttc cattcaatat ttatctaatt atttagaaga ctatgataga tttaactata
    16081 caaataaaat tataaaacta caaacagatt tcacgctgaa aggtacacag ttataccaca
    16141 aatgggaaga aaacataaat ttaggtcaag caaaggaact tattaaagca agggaacaag
    16201 aatacctaag aacttttcaa gaggccatag gttttaaaaa tggtatagtc caaaatctct
    16261 ttggaaaaga gatgaaaata tattttaatg aaaatgtaaa ttttttaaat tggaatgaag
    16321 ttgaaaaact attagaattt ttaatggaca tatcccttgt accagtaata attttagatc
    16381 aaatctttga ggataaaagt tctttcatta aacttttaga aagtttcata ttatatttcg
    16441 ctgatatata tggaattgag gtcgtaaaat tatggaaatt tcaagtttca aaggatttat
    16501 ctatagaata tatagatatt tcaaagcaaa ttttcgaaaa atatctatta caggatttaa
    16561 tagaagaacc ttttaatggt ccagaagcta taaatactat ttatgactct tcatatatgc
    16621 ttccatatat tattcataat ttcattaatg atactagcag tttgaatgca ctaaaggcct
    16681 ttgacaccgt agaagaaaac tcaattttaa ataacgaact gttttttgga tctcccggcc
    16741 ttcttacctt agatggaata aaaaaagctt cgtattatgc ctattattta ttgtctaaac
    16801 taggaaatga agttattgat aaaggcgatg gatatattat cacaagaaga gatgaagaca
    16861 tccaagtttt actttacagt tacagcgatg aacttaatac actaattaag ctagaggatt
    16921 tagctaaagg taagggccgt aaaaatatta ccgagagaaa aatatcctta tcaattaaaa
    16981 atttgattca tgactacaaa gttattaaat atgaaatagg agaaaaagtt ggatcagctt
    17041 atgatatttg gttacgaatg ggtaaaccaa aaagacttgg ttatgatgag tggaagcttc
    17101 taactaaaat ttctacccct aacatttcct tatcttatgc taaaaaaaac gcagtttata
    17161 ataatataat aaagatagaa ggttatggtt ctacattaat agttcttcaa gaagtacaaa
    17221 aacacctgtt ttagggtgtt tttttgttat ttgcgtataa cacaaagtct catgttagca
    17281 actttgtact ttttatctag caaccgtgtg aatgtaaaaa aatgcatagt agcgtagaat
    17341 aatatcatag aaacaaaaat ttttaaggag tagtggattt aatgacgata agtaatatca
    17401 cagaaagtaa caaaatgttt ggtgaggaaa aaatcgggaa actattattt aaattttctg
    17461 tgccagcaat aatttcttta atggtagccg aaatgtacaa catggttgat tctatgtttt
    17521 taggtcaagc tataggtgct aatgcgattg gtgctctaac catagccttt ccaattcaaa
    17581 gattgttttt tgccatatct atgcttatag ctatcggtgc ttcaacttcc gtttcaagaa
    17641 gttgtggaga caaagatttc gaaaatctta agataattat accaaatgct atagttctaa
    17701 tgtttataac aatatctata atcggtatta gcatttttat ttttcaagat aaaattctaa
    17761 ttagcctagg agcatctaaa aatacatttt ctcttgcgaa agattatata agtataatat
    17821 taattggtgt actatttcaa tcctttacta taatcgcatc gtatattata acttcttttg
    17881 gaaacacaaa aatagtttta ttaagcactt ctattggagc aattttcaat gtaattataa
    17941 attatttcct agtaaatgta ttctcatacg gagtaaaagg agccgctatc gcaaccgttg
    18001 tatctcaaat aatagctttt atatatgttc ttattgtatt tattcaatta aaaatgagcc
    18061 tgaaattttc ttttaaatta accctaaaca aagcagtatc tgttggaata gtatgtgttg
    18121 gattttcaac atttataata gaaatatcag acgcaatagt tgctgctata ttaaacaatt
    18181 tattattctc ctatggtgga gattcggcca tcgtaacaat tggtttaact acaagggttt
    18241 caatgttttt atttatgacc gtaatgggta taagcacagc tatgcaaccg atcgctgcat
    18301 ataattttgg tgcaggaaat tatacaaggt taaaagaagt tgttaagtcg tccataattg
    18361 cagtatctgt aagctcaact ttattatggg caatatttat gattttttca gatacattta
    18421 taggcctatt tataaatgat aaagccatta ttgaacaaac atctaaggcc tttagaatgg
    18481 ttatagctgt attcccttgc attggcgtat attatctatc aatatcttat tgccaagcta
    18541 taaatagagt taaggcatct ttcaagttat caatatttag gcagttaata gtatttatac
    18601 ccctcgtata ttcatttgta attggtctta atatgggtgt atccggagca tggctcgctt
    18661 atcccatttc agatgtgata tcctttatta cagctgctat atttattagg tacacttata
    18721 catctcttga aatactagag aggcatcaaa tttttaaact taataaaatt aaaataagac
    18781 ttgcaagcat tgcgagtact gctagttaat atacttctat aaaacaaaga catagcttta
    18841 taaaaaatcg atgtataaaa tatattataa catcgatttt taattatact tgctctattt
    18901 ctccctcttt tatcatattt atatctccat aaaattgtaa ttcattatta attacaacta
    18961 ttccatcccc atcattacat gtataaacta cattaccagt attattagaa tacttcttta
    19021 aatcatccaa atacttacta cacatattcc aatgtggcat aaaattaaaa tcaactagag
    19081 ctaaagatga tagatttgat aaacctacta tatcttcact tccaaattgt gcagctgata
    19141 tatcctttgc catcattatc gcacctgcgc atacccctat aatacaacca ccattttgga
    19201 catatcgtct taaccttgtt ataaaatatc ttttcttaag attatttaaa aaatagtaag
    19261 catttccgcc tgataaatat attacatcag acttaaatat tgcatctatt gtatctatac
    19321 tacaaaaatc atctatatca aaatacttaa attcaaaatt tccatattca cgcaattgta
    19381 tttttgcctt tttaaaactt tctaatttct tatctgtcat tgaaggtata taactcaaag
    19441 tatattttct atctaaaaat atttctttca tcctctttat taaattaaca ttatcctttt
    19501 ttgtaaaacc actgagcaaa actaacgcgc ccatattatt atcacctcac cttgatattc
    19561 ttataaaatt ttcattattt tatatattat accatattaa tgaaaaccta tacagagata
    19621 tatatatttt ttttagtcta tgagaatttc aaatacaata ttcctgctct actactaaca
    19681 tatggtcttt atcaaaaaaa gtgaatttat tatatattga catcttctaa aaatatatgt
    19741 ataattaaat ttaaatgtaa taaaagatat aagcatatat aatttcaatt taggtggtat
    19801 cgcgaaagtt tacctttcgt cctgttatat tttaggatga gaggtttttt attttaaatt
    19861 aaaatactta cataagaaag gatgattaat atggattatt taaaactggc tgatttatta
    19921 ttacctaacg taaaagaaac tccagaatac tatagaactc tttacccaaa gcgtaatctt
    19981 aaacctaatg ctatgattac aagatatgca ccaagcccta ctggttttca acatcttgga
    20041 ggtgtctttg ctgctctcat atctgaaaga cttgctcatc aaagtgaagg tatattttat
    20101 cttagagtag aggatactga caaaaaaaga gaggtaattg gtgccatcga agatagtact
    20161 aacactcttc gagattttgg aattaatttt gatgaaggtg ttatagattc tactagtgag
    20221 aaaggtgcat atggaccgta taaacaaagt gatagaatta atatatacca cgctttcgta
    20281 aaatctctaa taaaaaaagg tttagcttat ccttgctttt gcactgaaga ggatttggat
    20341 aaaacaagaa caattcaaca agaacttaag attaatacag gatatcatgg aaaatgggca
    20401 gttcacagga attcaacaat ggatgagatt aattctgaat taaataaggg caccccttat
    20461 gttataagac ttagagcacc tgaagcatct ggcaaaagag ttgtttgtaa tgatttaatt
    20521 aaagggacca taagtatgcc cgaaaatgac caagatattg taattcttaa aacaaacgga
    20581 attccaacat atcattttgc tcatgctata gatgattatt taatgggaac aacccatgta
    20641 attcgtggcg aggaatggct ctcatctctc cctatacatt tgcagttatt tgaaatgttg
    20701 ggctttacgc cacctcagta tgctcatata cctacaatta tgaaaatgga tggagcttca
    20761 aaacgtaagc tcagcaagag aaaagaccct gaaatttcta tgaattttta taaatctgaa
    20821 gggtatcctt ttacttctgt agctgaatat cttataaatt taattaattc tgattttcaa
    20881 gagtggaggg atacacacgc aaatattcct tgcacaagct ttaatattac ccttgaaaac
    20941 atgagcaaaa gtggagcttt atttgatatt ttaaaactta acgatattag taaaaatgtt
    21001 atagctacca tgaatgcagc tactgtatat gaactttatc ttaattgggc taaagattat
    21061 gatgttaaaa tgtcaaattt attaatagag aatgaagaat atgctaaaaa gatatttaat
    21121 atagaaagag aaaatgataa gccaagaaaa gattttgtta aatggttaga tgtaaaagaa
    21181 catattgagt atttctttga tgatctttat aaagatagcc ttactaaggg atacaatttc
    21241 cctgatcata taccaatgga tgaggtaaga agtgttcttt caagttatat cggtgtatat
    21301 gattattctc atgataatga tacttggttt gcaacactta aagaacttgc gttaaacctt
    21361 ggatatgcca aaaacgcaaa aacttataaa aaaaatccag agttatttaa aggtcaatta
    21421 tcagatgttg cgacaattgt aagagttgca ttaactaata agacacaaac tcaagatcta
    21481 catgaagtaa tgcaagctat gggtgaaaaa cgtgttatag aaagattaag ccaagatttt
    21541 aaataatata taataaaaga ggaaaccaat atttatttgg tttccttttt tatttatacc
    21601 ctaaacttga aattcgtcca ctgccgctgt aagctttact gccagattct ttaaatcttg
    21661 tgtatgtgaa acaatctgat ctatacttgc tagttgctct tctgttgtag ctgcaacctg
    21721 ctgagtcgca gctgaatttt cctctgttga agctgagagg gtttccaaaa tttcaattat
    21781 tacattcttc tctgtttcca tttctagact atatccctta atacttttca tatcctctga
    21841 aatcttacct atagcttcta gaatctgtac aaatatactt ttagcatcta ttacagattt
    21901 gctttgttct tttgctattt catttccaac ttccattgct ataactgcat ctttcgattt
    21961 tttctgaatt ccatcaatta gttctctaac ttttgcagca gatatagcag actctgaagc
    22021 gagctttctt acttcttcag caactactgc aaatcctttt ccctgttcac cagctcttgc
    22081 agcttctata gcggcattta atgctaataa atttgtttgc tctgatattt gactaattgc
    22141 ctgggttatt gctccaattt ctatagaact tttatcaacc tcaagtatta tatcacttac
    22201 ctttcctgat gccttgctat tctcatctgt tttttctata agttgttcaa atgcagaaaa
    22261 tcctttgctt cctatcacaa ccgttgcctc tgttacctca ttagttttta cagaaagcgc
    22321 tgttacaagt tctattttat ctgctaagat tttaacccta ctaactccat tttgtgtctc
    22381 taacgcctgc tcacttgat
//
