LOCUS       NZ_CP086140            12223 bp    DNA     circular CON 20-JAN-2024
DEFINITION  Clostridium estertheticum strain CF016 chromosome, complete genome.
ACCESSION   NZ_CP086140
VERSION     NZ_CP086140.1
KEYWORDS    RefSeq.
SOURCE      Clostridium estertheticum
  ORGANISM  Clostridium estertheticum
            Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5053333)
  AUTHORS   Wambui,J., Stevens,M.J.A. and Stephan,R.
  TITLE     Clostridia strains as spoilage organisms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053333)
  AUTHORS   Wambui,J., Stephan,R. and Stevens,M.J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2021
            Assembly Method                   :: unicycler v. 1
            Assembly Name                     :: CF016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Nanopore/Illumina miniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 05:10:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,249
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 5,019
            CDSs (with protein)               :: 5,019
            Genes (RNA)                       :: 135
            rRNAs                             :: 15, 14, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 13 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 22 of 95
            Pseudo Genes (incomplete)         :: 78 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 16 of 95
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:27:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 943120
            Orig. end                         :: 955343
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086140.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12223
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12223
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12223
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7223](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7223
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    1634..1643
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 14.61"
                     /tool="antismash"
     gene            complement(1834..>2355)
                     /locus_tag="LL065_RS04535"
                     /old_locus_tag="LL065_04530"
                     /pseudo=""
     CDS             complement(1834..>2355)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235606.1"
                     /locus_tag="LL065_RS04535"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_04530"
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="ELYPLKKTSISFLDPASYQQGISYQLTQSISGIGYGGLLGKGFFK
                     GIQIGVIPEIHTDFIFAAVGEEWGLIGAIGLLSLYGVMMCRIIKLAKSSENIAGSLICV
                     GTVASFLFSIFQNIGMTIGIMPIAGITLPFMSYGGSSMLTNFISLGLVLNVGIRRQKID
                     FRYIFRFICN"
     gene            complement(2373..2972)
                     /locus_tag="LL065_RS04540"
                     /old_locus_tag="LL065_04535"
     CDS             complement(2373..2972)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233883.1"
                     /locus_tag="LL065_RS04540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_04535"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_226137789.1"
                     /transl_table=11
                     /translation="MKREIRIPVQKRSIEKRKKIIDAALKIFNEKGYFNTNTAEIAKEA
                     ELATGSLYAYFKDKKDIFLEVTNLYGNAIYNHSVTELSKIKDTSDLDLIIKTTINIIIE
                     NHKIYSNFHQEMMTLCYIDNDIKYQVREQQKLLLNKYIEEFKFFNLNIKHSKEKYFLIY
                     SLIEDICHEIVYADKSILDRDVLIDECVKVIKKVLL"
     gene            3107..4615
                     /locus_tag="LL065_RS04545"
                     /old_locus_tag="LL065_04540"
     CDS             3107..4615
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441042.1"
                     /locus_tag="LL065_RS04545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_04540"
                     /product="MFS transporter"
                     /protein_id="WP_226137790.1"
                     /transl_table=11
                     /translation="MKKSKVILGFTALIIGCFLGVLDSTIVNIALPDMATYLGKNINDV
                     SWVTTAYLLSFSVFLIIGSKIADQFGRKKILIIGLFIFGLSSLLCGVGSSFIFLIIMRF
                     FQGIGAAIVTPVVLPLGLEIFGKEKRGFVIAVSGGITALAAAAGPPMGGIIIQYLNWKY
                     IFYVNVPMTIIAIVFAALSLNESYDTTVSKKVDVLGAILLMISIFCLVFSLLKGNDYGW
                     QSKTIVSLFCICIVSIITFLIIEVKTKEPMLPLNLFKESTFTASCILYMSAGFATASPL
                     LLLNFYLEEVFNYSPLKSGLVLMTLSLASVLGIPLGSILVKKIGSRLVNFSGILIVGVG
                     TVLLSQIDINTSITSMRITLVIMGIGFGFSVQSIASSIKYLPVEKSGIASGIINAARQI
                     GTCIGIAVLVSILNSNVTNATNNIKKDVTTQINSQANLQPIIKGKMLNKINNPNQDVIR
                     EIKNKELSKAFDNTFVVSYTIVFLLSICALFTDRKPTDIEKLIV"
     gene            5001..7223
                     /locus_tag="LL065_RS04550"
                     /old_locus_tag="LL065_04545"
     CDS             5001..7223
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075312.1"
                     /locus_tag="LL065_RS04550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_04545"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_226137791.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.5e-40, bitscore:
                     135.0, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 3.1e-211, bitscore:
                     701.6, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNIFKKYYCIKQHDYKDCGCACLATICKTYGLKYPISKIREASG
                     TDKKGTSALGIIKAAEQLGFSAKGVKASKPEDIFSGIPLPAIAHVVIDKTLLHYVVIHK
                     ISEKEILVADPAKGIVKYTPADFFNIWTGILIIMTPTVKFEKGNELKGVFSRFFDLIKP
                     QKGLLVNIFLSSILITIFGILGSFYFKLLLDDIVPNNLTQSLHMFSIGFIILITFKVIT
                     NAFRTQLLIHLGQRLDIPLMLGYYDHVINLPMNFFGTREVGEIISRFNDASKIRDAISG
                     VTLTMMIDVFMVIIGGSILYSQSSLLFGITLVPLILYGIIVFIFKKSIEKVNRETMESN
                     AKLTSYLVQSLTGIETIKAFNAEREVNAETEKRFIKLIKAVFKNGFINNFQVSLKGFVK
                     GIFSIVILWIGTLQVLGGKISFGELLTFNALLVYFLDPIENIINLQPTVQTAIVAAERL
                     SEILDLSLEKSIDENKKIVPETLKGDIEFKDLDFRYGTRSLILKNINLKVKSGEKIALV
                     GESGSGKTTLAKLLMNFYQCEKGELLINGYNIKDINVESLRDKTAYISQETFLFNGTII
                     ENLSLGNPYLTYEEIIEACKKAQIHDFINSIPLRYNTLVEENGSNFSGGQKQRLSIARA
                     ILRKPEILIMDEATSNLDSITERAIEKTIYEFSKDITTVIIAHRLSTIMRCDTIYILEK
                     GEIIERGSHTELIKQKGKYSELWKEQLPDSYEEEMVAAKEGEINEI"
     gene            7213..8643
                     /locus_tag="LL065_RS04555"
                     /old_locus_tag="LL065_04550"
     CDS             7213..8643
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452686.1"
                     /locus_tag="LL065_RS04555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_04550"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_226137792.1"
                     /transl_table=11
                     /translation="MKFKIESLDDLSESRQVMETEPNKFISIFVYILLAVIIAFILWAC
                     FSEKEVVVNVTGMVKPSGQSYVVSNPIAGEVKEVYMKNGQTIKKDEVLYKTDDSPLQTQ
                     RSKVEGQKKVLTNDFNNLGKLKKSIADSTNYFKESKEEKEYYYKYESYILGNKVSIEDK
                     TNLINSKNDSVNQINHLEKLIKSIEDNKNYNAKGSIYNEQFNNYQISRKSIDDKTQQLV
                     LNKKILQNKIGSGDAIAQIDAEIQDNKNGLIKLESDIKLEARKSINELRGQLKTANSNV
                     IKFDEGVNLSKEKNKITLLAQIEEKLNSNDEKIKEVDASLKEINKNIEKCKVKSTIDGK
                     LDIKANLQPGIIIQTGAIVANILPKSNAYKVELIIPDKDIANIKDGQEIKYSFTSLPYN
                     EYGFLKGSMESISVNSQTDSEKGIVFYKGEGMLKNNILYSHKNEEAVIKPGMTCDAKII
                     TRREKMLYYLLEKLNLKD"
     gene            9200..9841
                     /locus_tag="LL065_RS04560"
                     /old_locus_tag="LL065_04555"
     CDS             9200..9841
                     /EC_number="2.7.7.87"
                     /GO_function="GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /locus_tag="LL065_RS04560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LL065_04555"
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="WP_226137793.1"
                     /transl_table=11
                     /translation="MMKVLEPIKENFKVVAKCIKEGGVIISPSDTNFALTLNPWNEAAI
                     ERAFAIKNRPATSPLTLFVLNPNDWKKYGEAEDEEMVQLLVDAFWPGPLNIIVKKKNTV
                     PDRMLCGGDTVSISCLSNPVWRGMMNELDMPVSMTSANLSGKADGVLVDMELAIRQIGD
                     KVEYILKGEAQGTTKSSTIISLVDKPSITRLGDISAEDIKKVINIAPSVV"
     misc_feature    complement(9885..9898)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.68"
                     /tool="antismash"
     gene            9919..10122
                     /locus_tag="LL065_RS04565"
                     /old_locus_tag="LL065_04560"
     CDS             9919..10122
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LL065_RS04565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LL065_04560"
                     /product="hypothetical protein"
                     /protein_id="WP_226137794.1"
                     /transl_table=11
                     /translation="MIAPKLIGSIKNVIKRKYTIMIGEGIKALGLICLINGNDFTILII
                     GQILSGLGYSFTAGTDSILLKS"
     misc_feature    complement(10249..10262)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.37"
                     /tool="antismash"
     misc_feature    11420..11435
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.56"
                     /tool="antismash"
ORIGIN
        1 ggaacaaagg aaatgatgat ttctattact tatcctgtaa aacaaaatgg aaagttcata
       61 ggagctttgg gatatgatat tagtttaaca tcaatggggg atgttagaac aactcttgaa
      121 aaaggaaata gtaataaatt aattttcctt gataacaagg gaactctaat aagttcttca
      181 ctatttactc aaatgggtaa aaatgtagct ccaagttttt caggaaaata tgatgataaa
      241 ggaattgaag atattgtagc aaataaagag gattttagta aacaatattc ttgggctgat
      301 aaggtttata gtgataagaa aggaacaacg aatgcaacta tagcagggac caattatgat
      361 gcatcttatt caacgataga agggttaaat tggaaggtgg tttcattaaa gcccgaatca
      421 gtagtcacaa gtaaagtgaa ttatctagtt aagttaagta ttatagtttt agttgtgaca
      481 ttgatgatag tagttctatt agctgctata acaagtgata aattggtaaa aggtattaaa
      541 catattaaag acgtagttaa taaaacagca aagggtgatt tgacaatatt attagatata
      601 agatcgggtg atgaattaga agatttatca aatgacttta atgatatgac aaggaatcta
      661 aattctttaa tttcggagtt aaaccaaaaa tttaaagcag tacaagaaac agcaagtagt
      721 ttgattacta tatcaaaaca aaatagttta gcaattggag aggtaacaga gtctatagaa
      781 gagatatctt ctggaacaca aggacaaacc caacagattg agaatggagc tgctgctatc
      841 gtaagtcttg gccatgaaat tgaaacaatc agtgataaat caaatttaat agaaaaccta
      901 ttaaataaga ctacaaatga tattggtaaa ggtaataatg atgttagaac cctagaaggt
      961 tcatatgcaa aacttgaaac gtcacttgga atggtttctg agataattag tgaattaaat
     1021 aaaaaatcaa ataatatagc cgaagttaca aatgttatat cggatatttc agaacaaact
     1081 aatcttttat cattaaatgc ttctattgag gctgcaagag caggtgaggc aggtaagggg
     1141 tttgcagtag ttgcagagga agtaaaggaa cttgctgaaa aatctaaaga ttcgtcatta
     1201 aatattaaag agattattga tagcataata actgatacaa gttcggcagt tgaatttatg
     1261 aataaaacca atggaattaa tcaaattcaa aaggaagctg taacaaatat aaatatgtca
     1321 atgcaagaga tcacctcttc aattgatgag gtattgtccc aggttaaaga agaaattcaa
     1381 ggcataaaga aaaccactgc agataaggat aatgtggtta caatgataga tggtatatca
     1441 gaagttgcgc aagaaacttc tgcttctacg gagcagatag ttgcttctat ggaagaacaa
     1501 tctgcttcct cagaagaagt taataatcat gctaataatc ttatagtatt aattgaggaa
     1561 cttcaaggga aacttgaaat gttcaaattt gaataaatat tatattggtg gattcattaa
     1621 attaatttta gatgaagacg gcggtatagt tgatatttat tagcaattaa aatattaaaa
     1681 caatacccct aattgtagag tgatacaatt gggggtattg tttataatta atgaaaataa
     1741 cgtaagtgta atataacagc tggaatagga aagttaattg ctgttttaaa gcatcagcag
     1801 gttcaaatgt tgttccaaaa gtaacatttt agttcaatta caaataaagc gaaatatgta
     1861 tctaaaatca attttttgtc ttcttatccc tacatttaaa actaatccta aggaaataaa
     1921 attagtaagc atagaacttc ctccgtaact cataaatggt aatgttattc cggcaattgg
     1981 cataattcca attgtcatac ctatattttg aaaaatagag aataaaaatg aagcaactgt
     2041 acctacacaa attaaactac ctgcaatatt ttctgatgat ttagccaatt tgattattct
     2101 acacatcatt actccatata atgaaagtaa tcctatagct ccaattaaac cccactcttc
     2161 tccgaccgca gcaaatataa aatcggtatg aatttcagga ataactccaa tttgaatccc
     2221 tttaaagaat ccctttccta acagaccacc gtaaccaatc cctgatatag attgtgttaa
     2281 ttgatatgaa ataccctgtt gataagatgc aggatctaaa aaggaaattg aagttttctt
     2341 caaaggatat agctcaaagt gatgaattaa ccttataata atactttttt tattacttta
     2401 acacactcat ctattaaaac atctctgtct aaaattgatt tgtcagcata aacaatttcg
     2461 tggcatatat cttcaattaa tgaatatatt aaaaaatatt tttctttact atgtttaata
     2521 tttaaattaa aaaatttaaa ttcttcgata tatttattga gcaaaagttt ttgttgttca
     2581 cgaacttggt atttaatatc attatctata taacataatg tcatcatttc ttgatggaaa
     2641 tttgagtata ttttgtggtt ttcaattatt atatttatag tggtttttat aattaaatcc
     2701 aaatcacttg tatcttttat tttactcagt tcggtaacac tatgattata aatagcattt
     2761 ccatataaat ttgtgacttc tagaaatata tcttttttat ctttaaaata tgcatacaga
     2821 ctacccgtag ctaattccgc ttccttagca atttcagctg tattggtgtt gaaataacct
     2881 ttttcattga atatttttag tgcggcatca attatttttt ttcttttttc aattgatctt
     2941 ttttgaactg gtattcttat ctctcttttc ataatatcac ctagttagat tttaaggtaa
     3001 agcgtatctt ttgtcaacaa tatgaaatta atttcatgtt cttgacatcg tgaaacataa
     3061 agcatacaat acgaatatga aataagtttc acattaggag ggttttatga aaaaaagtaa
     3121 ggttatttta ggttttacag cactaattat tggttgtttt ttaggggttc tggatagtac
     3181 tatagtaaat atagcattac cagatatggc aacttattta ggcaaaaaca ttaatgatgt
     3241 aagttgggtt acaactgcgt acttgttatc attttcagta tttttaatta taggttctaa
     3301 aattgcagat caatttggaa ggaaaaaaat tttaataata ggattattta tatttgggtt
     3361 atcatcacta ctctgtgggg tagggagttc atttatattt ttgatcatta tgagattttt
     3421 tcaagggata ggcgctgcaa ttgtaactcc cgtagtttta cctctaggac ttgaaatttt
     3481 tggcaaagaa aaaagaggtt ttgtaatagc tgtatcaggt gggattacag ctttagcagc
     3541 cgctgctggg ccacctatgg gcggaataat tatccaatat ttgaactgga aatatatttt
     3601 ttatgttaat gttccaatga ctattatagc tatagttttt gcagctttat cgttgaatga
     3661 atcttatgat actacagttt caaaaaaagt agatgttcta ggtgcaattt tacttatgat
     3721 aagtatattt tgcttagttt tttcacttct taaaggaaat gattatggat ggcaatcaaa
     3781 aacaatagta tcactttttt gcatttgtat tgtatcaatt attacatttt taattattga
     3841 agttaaaact aaagaaccaa tgctaccact taatttattt aaggaaagta catttacggc
     3901 ttcttgcatt ctttatatgt cagcagggtt tgcaacagct tcgccacttt tattattaaa
     3961 tttttatcta gaagaggtat ttaactactc tcctcttaag tctggccttg tactgatgac
     4021 tctttcacta gcttcggtat taggtatccc tctaggtagt attttggtta aaaaaattgg
     4081 atcaagactt gttaatttct caggaatact tattgttgga gtaggtacag tgttgttatc
     4141 gcagatagac ataaacactt caataacaag catgagaata acacttgtta ttatgggaat
     4201 tggatttgga ttttcagtac aatcaattgc ctcatcaata aaatacctac ctgtagaaaa
     4261 aagtggtata gcatcaggaa ttattaatgc tgcaaggcaa attggaacgt gtataggaat
     4321 tgcagttctt gtgagtatat taaatagtaa tgtcacaaat gcaactaata acattaaaaa
     4381 agatgttacg acccaaataa attcacaagc aaatctacaa ccaattatta aagggaaaat
     4441 gttaaataaa attaataatc caaatcaaga tgttattaga gaaattaaaa ataaggaact
     4501 aagtaaagca ttcgataaca ctttcgtagt ttcctatact atagtttttt tattgtccat
     4561 atgtgcatta tttactgata gaaaaccaac ggatattgaa aaactcatag tataaatttt
     4621 tcaacataaa aatgctcccc tttctaaatt tgtctagttt aaggggagca tatcactttt
     4681 aatttcgcat tttgtaggtc tcattaatca aataatatca taacattaaa ttattcagtg
     4741 catgtccaac ttttactcct aataaaaata ttaaagcacc tatacctata attaaacaag
     4801 ctttaataaa ggggttgtca ttaatgaaag atataatatg aaaaaagatt ttatttttta
     4861 ctattaataa atccacaata tcacctcttt ttagataaat ttatacatta tacgattaat
     4921 tttacaatgc aatcatagca taatttatca taatttgcta taattatgat aaattaatca
     4981 tggaaaatgt gaggtgtata atgaaaaata tatttaaaaa atattattgt attaagcaac
     5041 atgactataa agattgtggt tgcgcttgct tagcaacaat atgtaagaca tatggtctaa
     5101 agtatccaat atctaaaata agggaggcgt ccggtactga taaaaagggg acaagtgctt
     5161 taggaataat taaagcagca gaacaattag gtttttcggc taaaggggtt aaagcgagta
     5221 aaccggaaga catatttagt ggaatacctt tgccagctat tgcgcatgta gttatagata
     5281 aaactttatt acactatgtg gttatacata aaataagcga aaaagaaata ctagttgcag
     5341 atccagcaaa aggaattgtt aagtatacgc cagcagattt ttttaatata tggacaggta
     5401 tacttattat tatgactcca actgttaaat ttgaaaaagg aaatgaactt aaaggagtat
     5461 tttcaaggtt ttttgatttg ataaaaccac aaaagggatt attagttaat atatttttat
     5521 catctatatt aataaccata tttggcattt taggttcgtt ttatttcaag ttgttattag
     5581 atgatatagt accaaataat ctaactcaat cattacatat gttttcaatt ggatttatta
     5641 ttttaattac atttaaggtt attacaaatg cgtttagaac tcagctttta attcatttag
     5701 gtcaaaggtt agatattccg ttaatgcttg gatattatga tcatgtaatt aatttaccaa
     5761 tgaatttttt tggaactaga gaagttggag aaatcatatc aagatttaat gatgcttcta
     5821 aaataagaga tgctatttca ggggtaactc ttactatgat gatagatgtt tttatggtta
     5881 taataggtgg atcaatatta tattctcaaa gttctttatt gtttggaatt actttagtac
     5941 cattaattct ttatggaata atagttttta tatttaaaaa aagtattgaa aaagttaaca
     6001 gagaaacaat ggaaagtaat gcaaaactaa cttcttattt agttcaatca ttaactggga
     6061 tagaaactat aaaagctttt aatgcagaaa gggaagttaa tgcagaaaca gaaaaaagat
     6121 ttataaaact tataaaagct gtatttaaaa atgggtttat aaataacttt caggtatcat
     6181 taaaaggttt tgtaaaaggg atattttcaa ttgtaatact ttggattgga acattacaag
     6241 tattaggtgg gaaaataagt tttggggaat tactaacttt taatgcttta ttagtatatt
     6301 tcctagaccc aatagaaaat ataatcaact tacaaccaac tgtacaaaca gcgatagtgg
     6361 cagcagaaag gctttcagaa atactagatt tatcattaga gaaatccatt gatgaaaata
     6421 aaaaaatagt tcctgaaaca ttaaaaggtg atatagaatt taaggattta gattttagat
     6481 atgggacaag aagtcttata ttaaaaaata taaatctaaa agttaaatct ggagaaaaga
     6541 tagctttagt tggtgaaagt ggctctggaa aaactacatt agcaaaatta ctaatgaact
     6601 tctatcaatg tgaaaaaggt gaattactta taaatggata taacataaaa gatattaatg
     6661 ttgagtcatt aagagataaa acagcctata tatcacaaga aacattttta tttaatggaa
     6721 caataattga aaatttatca cttggaaatc catatttaac atatgaagaa attatagaag
     6781 cttgtaaaaa ggctcagata catgacttta taaactcaat ccctttaaga tacaatactt
     6841 tagtagaaga aaatggttct aatttttcag gagggcaaaa acaaagatta tcaatagcaa
     6901 gagccatact tagaaagcct gaaattctta ttatggatga agcaacaagc aacttggatt
     6961 caataacaga aagggctatt gaaaaaacaa tatatgaatt tagtaaggat ataacaactg
     7021 taataattgc acatagatta agcactatta tgagatgtga tactatttat attttagaaa
     7081 aaggagaaat tatagaaaga ggttctcata ccgaacttat aaaacaaaag ggtaagtatt
     7141 ctgagttatg gaaagaacaa cttccagata gttatgaaga agaaatggta gcagctaagg
     7201 agggagaaat aaatgaaatt taaaatagaa agtttggatg accttagtga aagtaggcag
     7261 gtaatggaaa ctgaacctaa taagttcata agtatatttg tttacatttt attagcagta
     7321 attattgctt tcatactatg ggcgtgtttt agtgaaaaag aagttgtagt taacgtaacc
     7381 ggaatggtaa aaccaagtgg acaatcttat gtagtttcta acccaatagc tggagaagta
     7441 aaagaggtat atatgaaaaa tggacaaact ataaaaaaag atgaggtttt atataaaacg
     7501 gatgattccc cattacaaac tcagaggagc aaagttgagg gccaaaaaaa agttttaaca
     7561 aatgatttta ataatttagg taaactaaag aaaagcattg ctgatagtac taactatttc
     7621 aaggagagta aagaagaaaa agaatattat tataaatatg aaagttatat tcttggtaat
     7681 aaagtgtcaa ttgaagataa aactaattta ataaactcta aaaatgattc agttaatcaa
     7741 ataaatcatt tagaaaaatt aattaaatca atcgaagata ataaaaatta taatgcaaaa
     7801 ggaagtattt ataatgagca gtttaataat tatcaaattt caagaaaatc tatagatgat
     7861 aaaacacaac aattagtatt aaacaaaaaa atattacaaa ataaaatagg ttctggtgat
     7921 gcaatagcac aaattgatgc tgaaatacaa gataataaga atgggttaat taaattagag
     7981 agtgatatta aattggaggc taggaagtca atcaatgaat taaggggaca attaaaaact
     8041 gctaatagca atgtaattaa atttgatgaa ggagtaaatt tatcaaaaga aaaaaacaaa
     8101 ataacattac tagctcaaat agaggaaaag ttaaattcaa atgatgaaaa gataaaagaa
     8161 gtggatgcta gcttgaagga aattaataaa aatatagaaa agtgtaaggt taaatctact
     8221 attgacggaa agcttgatat taaggccaat ttacaacctg gaataataat tcaaactggg
     8281 gcaatagttg ctaatatttt gcctaaatca aatgcttaca aagttgaatt aattatacct
     8341 gataaagaca ttgcaaatat aaaggatgga caggagatta aatatagttt cacttcatta
     8401 ccatataatg aatatggatt tttaaaaggt agtatggaaa gcataagtgt taattctcaa
     8461 acggatagtg aaaaaggtat tgttttttat aaaggagaag gaatgttaaa aaataatatt
     8521 ctatatagtc ataaaaatga agaagcagta ataaaaccag gaatgacatg tgacgcaaaa
     8581 ataattacac gaagagagaa gatgttatat tatttactag aaaaattaaa tcttaaagat
     8641 taatttttct agtaaaagtt cttctcatta aatgataatt gtatttgtaa ttaatacacc
     8701 caaataatgg aggtgtattt tttattttgg agtaattttc gttagtagtg tagttaatat
     8761 aaacagtctt cctaagtcta atattttaaa atattcggcg cgtaaaaaga gataaccaat
     8821 aagcatactt gtctgttatt tctttttacg tgccttaaat agcaataata gctaatgaaa
     8881 aaaactattt gaaactatta tatatagtat gatagtataa atcgaaaagt aaatcaatgt
     8941 aataaatgag aaaaatttaa ttaatcaact ataaataaac attttgcttc gatagtttta
     9001 cagttgaata actaaacaac tatccatatt aattttaagc gattattaaa gttataaacg
     9061 catcagagta ttatcatcaa caaatgtaat ttttaactgt aaatacattg atagacattt
     9121 tactttattt acctgtaaaa atattagagt attgttgcaa tattataggt atgaaaccaa
     9181 aaaaaattag gaggcaatca tgatgaaagt tcttgaaccc attaaagaaa attttaaagt
     9241 agtagcaaaa tgtattaagg aaggaggcgt aattatatca cctagcgata ctaactttgc
     9301 attgacatta aatccatgga atgaagctgc tatagaaaga gcttttgcaa taaaaaatag
     9361 accagcaact tcgccactaa ctttatttgt tttaaatcct aatgactgga agaaatatgg
     9421 tgaagcagaa gatgaggaaa tggtacaatt gttagtagat gcattttggc caggaccttt
     9481 aaatatcatt gtaaagaaga agaatactgt tccagataga atgttatgtg gtggcgatac
     9541 agtatctata agttgtctta gtaacccagt ttggagagga atgatgaatg aactagatat
     9601 gccggtttct atgacttcag ctaatttatc gggtaaagcc gatggagttc tggtagatat
     9661 ggaattagcc attaggcaaa ttggtgacaa ggtagagtat atcttaaaag gggaagctca
     9721 agggacaaca aaatcaagca cgataatttc acttgtagac aagccaagta ttacaagact
     9781 tggagatata tctgcagagg atattaaaaa agttataaat atagccccct cggtagtata
     9841 gtatttgttt tcttttattt aaaggggcta tctatccttc aaatagaact tatgttagca
     9901 atttatggat tagtacttat gattgcacca aagcttattg gcagcattaa gaatgttatt
     9961 aaaagaaaat atacaattat gattggtgaa ggtattaagg cattaggtct tatttgcttg
    10021 ataaatggga atgattttac aatattaata atagggcaaa tattatctgg tttaggatat
    10081 agcttcacag ctggaactga ttctatatta ttaaaatctt agtgaattgt ttattgagaa
    10141 attgaaatcg tcacttaaaa tatgtggtgg taagatgatt cttttatctg ctgtattacc
    10201 aaatttaaag gacttatatt tcgactatta ggtgtttatt caattataag aacgaatgtt
    10261 tttataattt ttatatccaa aattatagtc caatatgtaa aaaaaatcac tatttaaatt
    10321 ataaatagtg attttttata tataatttgt aatgatgaat aaatattcat tttacagaac
    10381 acaacttagt tgtatttata ctctattgat gcgtatcatg tatagctgtt aactacatcc
    10441 ggtagttgtt ccacaatcaa gacaaacttt acaagtacca ttgcgaacaa gcctagtact
    10501 tgagcatagt gagcaggttt cagagtacaa tctttcacct tgaattttgt cagtttcttt
    10561 acttacagga gacgccacat tagcggattc ttctaaagta gttgaaattt cattttcaac
    10621 agggattaac ggattattag gtatatttgt gctctcgtaa ttgtcaggtt taatttgaca
    10681 tctactgtag tcacctaatt ctatatcaat aattttgcta atcaaatcag aaatagatga
    10741 aactgattta atataaggat gtttttgtac aaaaccataa ggctcaaatt tttgacctcg
    10801 aagcatcgtc gagatagctt ctgcaggtac atggtgttga agcataactg agattgcttt
    10861 tgataaagaa ttaagtaaac cagtaataat agtaccttct ctatctgttg ttatatatat
    10921 ttcagaaatc tctccatttg aacgtctatt tacagtagta taaatcttta catcttcaat
    10981 ttgagcagga tgagtagtac cataacgtat cccaattggt ttttcacgtg ttgagtgtga
    11041 ttgatttgaa gttttttgta atgatcttgc tttctttaaa agagcgtcat atgatagatc
    11101 ttcaagtaat agttccttat cctcggacaa tgtagtgttt agtggttgac ttgcctttga
    11161 accatcccta tataaggcta taccttttac tcctaatttc caagaagtta atataacttc
    11221 tttaaaatct ttaatagtgg catttttagg taagtttaca gtctttgata tagaacctga
    11281 tatcattggc gttattgctg ccatcataag aacgtgtccc ataaaggaaa tatacctttt
    11341 accgctacca catttatttg ctgtatcaaa tatagataaa tgttcccgtt tcaaatgagg
    11401 agcaccctct attttgccgt ccacaatttt ttcatattca aagccgtttt catcaaccat
    11461 ttcttttctt agaatgtaat caaaaatatc atttgtttca ctttctgaat atccaaggtt
    11521 agcaagagaa tctttaatta caggatttac gatggtcata aaaccgccac cagataattt
    11581 cttataaata aaatgactga agtatggctc tacagatgtt gcaccacaat ccatagcaaa
    11641 ggaaatagtg ccagtaggag ctaaaactgt aacctgagcg tttctaaaac catagttttc
    11701 gccgctgctt aatgcttttg tccagcaact ttttaaggtt tcacttatag cacctaaaga
    11761 gtctttattt aaaatagcgt gatccacttt aataggttca tagtttagat tttcaaagtc
    11821 actctttatt gctccagctg ctcttgcatg atttcttata actatctgca tgtattcttt
    11881 attaatatca tatttttcaa aaggagataa ttctttagca atcaaagacg aaacataata
    11941 agactgcgca gtcattatcc cggttagtga tgatgctata gttctagcgt cttctgagtc
    12001 atatggatag cccattacca taaataatga agctaaattt gatagcccaa gaccagtggt
    12061 tctaaataaa tgagtttttc ttgctatatc ttcagttggg aattgtcccc aatgtattga
    12121 tgcttcaaga actaattgag taaggcctat taaatgcaca tatccatcta tatcaaattt
    12181 tttagttaaa gtgtcataaa atttataaat attaatagac gct
//
