LOCUS       NZ_CP113114            10297 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Streptococcus pneumoniae strain NP1 chromosome, complete genome.
ACCESSION   NZ_CP113114
VERSION     NZ_CP113114.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Genome sequencing and assembly of Streptococcus pneumoniae strain
            NP1 isolated from nasopharynx
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2022) Community Medicine &SPH, Post Graduate
            Institute of Medical Education & Research, Chandigarh, Room No 529,
            Research Block B, PGIMER, Sector 12, Chandigarh, Chandigarh 160012,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: MaSuRCA v. 3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 356.0x
            Sequencing Technology :: Illumina Novoseq 600; Nanopore GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 02:39:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,409
            CDSs (total)                      :: 2,335
            Genes (coding)                    :: 2,154
            CDSs (with protein)               :: 2,154
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 86 of 181
            Pseudo Genes (incomplete)         :: 87 of 181
            Pseudo Genes (internal stop)      :: 72 of 181
            Pseudo Genes (multiple problems)  :: 53 of 181
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:47:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2031168
            Orig. end                         :: 2041465
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(67..945)
                     /gene="rsgA"
                     /locus_tag="OTU45_RS10405"
                     /old_locus_tag="OTU45_10405"
     CDS             complement(67..945)
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0043022 - ribosome binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rsgA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /locus_tag="OTU45_RS10405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10405"
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="WP_001161981.1"
                     /transl_table=11
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVDF
                     SAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHKGI
                     HPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGVGKS
                     TLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVSRAED
                     LNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEIENRRE
                     TYKKVSKKIPK"
     gene            complement(947..1819)
                     /gene="rsmA"
                     /locus_tag="OTU45_RS10410"
                     /old_locus_tag="OTU45_10410"
     CDS             complement(947..1819)
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="OTU45_RS10410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10410"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_050117944.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQTGLSPSVRGEALSLAEFAGL
                     ADALKGQGL"
     gene            complement(1950..2258)
                     /locus_tag="OTU45_RS10415"
                     /old_locus_tag="OTU45_10415"
     CDS             complement(1950..2258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /locus_tag="OTU45_RS10415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10415"
                     /product="hypothetical protein"
                     /protein_id="WP_000333257.1"
                     /transl_table=11
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYLL
                     DFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC"
     gene            complement(2251..2892)
                     /locus_tag="OTU45_RS10420"
                     /old_locus_tag="OTU45_10420"
     CDS             complement(2251..2892)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="OTU45_RS10420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10420"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000565531.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(2894..4924)
                     /locus_tag="OTU45_RS10425"
                     /old_locus_tag="OTU45_10425"
     CDS             complement(2894..4924)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="OTU45_RS10425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10425"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000825251.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMINQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLLSLA
                     IFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSLLGLG
                     ILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVHLLGLQENSLVDLLKGKLPLKR
                     MMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGWSSAFAD
                     EEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYIPSGNVIYV
                     SPRYLIEEKITVSSEFIDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYLQNFSSESV
                     EVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGTRPVAGML
                     WGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRVEFYSLLIGTI
                     LTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGGVLLLGLVLSSI
                     LTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(5001..5297)
                     /locus_tag="OTU45_RS10430"
                     /old_locus_tag="OTU45_10430"
     CDS             complement(5001..5297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="OTU45_RS10430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10430"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815394.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 6.8e-20,
                     bitscore: 68.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNGG
                     AYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     misc_feature    complement(5447..5460)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.05"
                     /tool="antismash"
     misc_feature    complement(5537..5546)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(5617..6528)
                     /locus_tag="OTU45_RS10435"
                     /old_locus_tag="OTU45_10435"
     CDS             complement(5617..6528)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904843.1"
                     /locus_tag="OTU45_RS10435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10435"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_000904830.1"
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPSL
                     PKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEIED
                     LFYEQLPNEEKVWFEATRATIDVIRSGRPEYGETVLDDYFKTIYDKELFLINELEVINL
                     YFAIVLTKIKQGQNQIEEINRIHSFLVRLTNHVELIASEYLFALSNTLFSGLACLDNLS
                     SYDSLGAYIFSLNHIMEKTQDFQKKPIILMLEWKLSLIINNDYVSAEQFYQKSKLFADI
                     IENSYLVTMLEKQWQEDLKKYL"
     gene            complement(6600..7160)
                     /gene="rnmV"
                     /locus_tag="OTU45_RS10440"
                     /old_locus_tag="OTU45_10440"
     CDS             complement(6600..7160)
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="OTU45_RS10440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10440"
                     /product="ribonuclease M5"
                     /protein_id="WP_000660013.1"
                     /transl_table=11
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(7160..7933)
                     /locus_tag="OTU45_RS10445"
                     /old_locus_tag="OTU45_10445"
     CDS             complement(7160..7933)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="OTU45_RS10445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10445"
                     /product="TatD family hydrolase"
                     /protein_id="WP_000575616.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIER
                     ALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTAPK
                     EVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVDLGMTISFSGVVTFKKATDLQEAAKELLLDKILVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(8238..8927)
                     /locus_tag="OTU45_RS10450"
                     /old_locus_tag="OTU45_10450"
     CDS             complement(8238..8927)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099757.1"
                     /locus_tag="OTU45_RS10450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10450"
                     /product="DUF1542 domain-containing protein"
                     /protein_id="WP_229293809.1"
                     /transl_table=11
                     /translation="MTFPDGSVVTILGKDTVQQSAKGESVTQEATPEYKLENTPGGDKG
                     GNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDE
                     IKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPV
                     APNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLG
                     RKTRRRK"
     gene            complement(9166..9300)
                     /gene="rpmH"
                     /locus_tag="OTU45_RS10455"
                     /old_locus_tag="OTU45_10455"
     CDS             complement(9166..9300)
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /locus_tag="OTU45_RS10455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_10455"
                     /product="50S ribosomal protein L34"
                     /protein_id="WP_000831905.1"
                     /transl_table=11
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA"
ORIGIN
        1 tcaaagttgg cataatctgc tgccagaatt gacggagcaa tcttgtattg agacataggt
       61 ttctccttat tttggaattt ttttgctgac ttttttatag gtttctctac gattttcaat
      121 ttcactaagg aattgcaggt aattgtcaaa acggaaggtt gcaataacac cctcttcaac
      181 agctggtttg acggcacaag acggctcatg ggtatgggta caagtacgga acttacaatc
      241 tcggctaaca gtagcaatct ctgggaaagc ctgattgagg tcttcagccc ttgatacttc
      301 atagtccaag gatgaaaatc ctggtgtatc tgcgatttta cccccgttga gattgtaaaa
      361 actaacagct cgagtggtat ggcgaccgcg acctagactg tctgaaattt ctcccgtttc
      421 aagattgagg tctggtgcga ttttattgag aagagttgac ttcccaacac ctgtctgccc
      481 cataaagacc gtaaccttgc ctgttaacaa agacaggagt tcctctttac tggtcacaaa
      541 gtcatagccg atgtcaccat aggtctgctg gtaaaaatcc agttctcccc tatcttccaa
      601 caaatccatt ttggaaatat agacaatggg atggatgccc ttgtgctcca aaagaaccaa
      661 gaaacgatcc agcaaattgc tgttaaaatc aggttccttg acggacatga ttactacagc
      721 ttgatcgata ttgacaatag gcggacgaac cagactgttt ttccgttcgt gaattttgag
      781 gatatagcct tctgaatttt cctcggcaga gaaatctacc cagtccccaa cataaggggt
      841 atggcctttt ttacggaaat tcccacgcgc gcgtgtttga taaacctggc catcactctc
      901 cacatagtag aaacctgcca aggctttaat gatttgtccc tgcatcttag agtccttgcc
      961 ctttaagtgc gtctgctaga ccggcaaatt ctgccaagct gagagcttcc ccacgcacac
     1021 ttggtgacaa gcctgtctgg tccaaagcct tggtcagctt gtccttgacc tcttcagtct
     1081 taccaaagta acctgtcaag ttattccaca aggtcttgcg gcgatgggta aaactagcct
     1141 tggaaacctt aaagaaaaag ttctcatctt ctactgctac ggctggctct ggacgacgca
     1201 ccattttcaa gatggctgaa tccacatttg gcgctggcac aaagaccgta cgaggcacga
     1261 taaaggcaac cttggctgtc atgtaatact gcacggcgat agacaagcta ccgtaagcct
     1321 tggtgttagg ctgggctgaa atgcggtccg ctacttcttt ctgcatcatg accacaaact
     1381 cacaaaaagg aatgccactc tcaatcaagt gcatgagaat aggcgtcgtg atgtagtaag
     1441 gcaaattagc cactaccttg attggcaggt caggattttt aaaattctgg atatgttgcg
     1501 ccaaatcaac cttgagaata tcttcgttaa ctacagtcac attatcaaaa tcacgcaggg
     1561 tatctgccaa aattggcacc aaacggtggt caatctcaaa agccatgact tgggctgcac
     1621 gctcagccag aaactccgtc aaggcaccaa tacctggccc gatttcgatg acattgacct
     1681 ggtcatcaat ttcagccgta tccacaattt tttgaaggat attggtatcc gtcagaaaat
     1741 tttgcccgaa ggacttttta aaggtaaaac cgtgacgctc cagcactgcc ttggtcacgc
     1801 tataatctgc aattctcatc tattctcttt tctattatct gttactacta ttgtaacaca
     1861 ttccccttgt attttgggaa tgaaaatcat accaaaaaac tatctttcct catgaagaaa
     1921 gatagtcttg gaaatcttta aaagtggcct taacagttct ctcgaaacat gcgttcaaaa
     1981 aaatcagctt cttgataact tcgattttca ggtagggtca aatccacatc ctcctccaca
     2041 tcatccactg tcagttcaac cgtctgaggt aaatttttca aacgataatg cttttgcctt
     2101 tgcccttcaa tactagcaaa atcaagcaaa tagccatgca ctcggatttg aaccaaaagg
     2161 agatgattgg tcatattttc catgatgagg atatcttcta aaccacctct ggcactagga
     2221 taatcagata ccctgcgaat acggattttt tcacacatga gcaagtttcc tcatatcaat
     2281 gatttcatca gccttattcc agactagggg attatgggtc gcaatgatga taattcgatt
     2341 ttcatctttc aaatccacca agagattcat aacctcctct gaattttcag gatcaagagc
     2401 tgctgttggt tcatctgcca aaatcaaggg tggatttttc aaaatagtct tagcaagggc
     2461 aactcgttgg gcctctcccc cagataaagt ataaattttt tgttctaaat ccaaataccc
     2521 tagattaact ttttctaaag cccccacttg cctttccaaa cgttctactt ttgagatttt
     2581 ctgaccaaca aaacccaaat ccaaattttc tttgattgat tggttttcta agaggccaaa
     2641 attttgaaag agatagccca tctgatctcg aaaatactca cgagtgggaa tggtttttaa
     2701 atctttcccc tgatagagaa cccttcctcc atctatcttt tctagctttc ccaagatatt
     2761 cagcagcgtc gtctttccgc ttccactctt tccgattaag gcataaacct tgcccttctc
     2821 cagcttgaga ttcaaaccag agaaaatcgc gcggtcatta aatttctttt ccaatccttg
     2881 aatatcaatc atcttatttt cctttcaata ctgccatgct accagcttct tcttttttgt
     2941 cctgccttac taaaatcaag agggcgttaa gcgtaaacaa agctacaact agagcgctaa
     3001 tcaaaccatc tcttgtcaaa atactagata ggactaggcc aagcaagaga actcctcctt
     3061 gcgccagtaa atacttgcca tgaagctcat agattgtcat accagcaaga cgtttaatca
     3121 taagctcccg tctgaactgc tcaaaataga gaagattcat ggaatcaaat aacaagatag
     3181 ccgtagacag ggtcaaaatc gtcccaataa ggagagagta aaactccacc cgtttgtcat
     3241 ttagtacctt ggcaaaaaat agctggcttt ttaccaagta agaaaccttg tgatacagac
     3301 cttgctcttt tagagctgtg atgctgtctc catatcgatc tagtttcaag gcactattag
     3361 ccgtagttcc ccacaacatc cctgcaacag gtcttgttcc agtcgcttgc ggcgttagaa
     3421 ctacaataat cggatctttt aggtactggc gtgttgtctt gtacccatca ttatagagga
     3481 aacgttcctg tcctgtttct gtaaaagcta gccttacctg tgggaagtag tgtttctgac
     3541 tcgtcacttc tacactttca gatgaaaagt tttgcaggta atctgtaaac aatccttggt
     3601 agtaggcaga ctgctctcgc aagctctcag gcaagatcag cccaaactct ccctcagaca
     3661 agttttgcat cttgtcaata aactctgaag aaacggtaat cttttcttct atcagatagc
     3721 gcggcgagac atagataaca ttccctgacg gtatgtagtc actgagacga ttgccatcta
     3781 ggtccacttc tgctccatct gagaaattgt caacattgct cataatataa aaagagtctg
     3841 tattggctaa ccgttcttca gtaaatgtct gccactcacg attatcctta cgcgttcctt
     3901 cttcatcggc aaatgcacta gaccaaccaa aggatagacg gtaacggtct gaggactggc
     3961 tccatttatt gctagctctc tccatttcct gcatttcacg gtagtggggt aggagagctg
     4021 tcgcactcga tccgaccacc aatacagcta agagttgccc caccatcatc aatgtcatca
     4081 tccgtttgag agggagtttc cctttcaata gatccaccag actattttcc tgtaaaccaa
     4141 gtagatggac gacacttagt aaggtagaaa tccctgccag ggtcaatcca taaagtagaa
     4201 gagcaatgat gaccagttgt accgttgcca taaacaaggc accttgatac cagagaatcc
     4261 ccaatcccaa aagactggat accagcactg agcagataag ctgtctcaca tcttctaaca
     4321 ctggtctgag agcaactcca aacaagctct caccagctat taagcgaatc cctgcctgac
     4381 gaagggattt gatccgataa atcagggtca gactcataaa ggtcagcaga aaaatagcta
     4441 aactcagtag caccataggg gtggccgcca atagtaagac tatcgaaaat ggatcttctc
     4501 cattcgaaac aaagccttga tagccaagtt ctttcaaggt ggtctgtaag ctcactccat
     4561 ccaaacttcc tgacacaatc aagtaagacc ctaataaatc actcgtttct gcactctcct
     4621 tggaggaaat ggtcagacct tctggaagct ttccctcacc ataagtggca taggtaaagc
     4681 gtgtttctcc agcttcattt ggctcaacga ttcgacgggc gatgagacta ttatgctcta
     4741 ccgccaagtg actcaaagcc tctgtcacct cttcgcgact agctactggt tgatttatca
     4801 tcattcctgt cacatcaaca gaaggatagt taccaaataa aatctcttct cgatggtcaa
     4861 cggaagttat caccatataa agcgatacca ataccattga aatcaaaata aataaacgtt
     4921 tcatcatttc ctccaaaaag ttacttggtc acttagtgag aaggtgacca agtaaagcta
     4981 aacaataggg ctataccatt ctaaacatta tagtagaatg caactttctc tccccaagaa
     5041 gtacgaatcc atgcacgaga agtccctcca gcaccagcat atcccttgtc agatgaacca
     5101 gtttttctgc taacaactgt tgaactatgg tattttgaag ggtgatagta gtttgagtaa
     5161 gcaccaccgt tacctgaacc ataagtccaa acaccacctt ctgggtattg aacggctgca
     5221 gaggcaactg ctacacttcc acctaatgtt gctgcaatgc tcgctacaag agctaatttt
     5281 ttaactgttt gtttcatatt gatactcctc tatttttgat tacaagattg ttgagttact
     5341 tgctcctctt tccattatca acgttgatac tattatatta ccaaatgata ttctaaaatt
     5401 atttgttttt ctctaaattg tcaaaatagc accctgaaat gaacttttat agtcattata
     5461 ctccatcttt atcacactat caaggacatc aatgtcctta aaatctaact atttcatatt
     5521 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     5581 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt ttaaatcttc
     5641 ttgccattgt ttttctaaca tagtaactaa ataagaattt tctattatat cagcaaatag
     5701 cttcgatttc tgatagaact gttcggcaga aacataatca ttgtttatta ttagagataa
     5761 tttccactcc aacattaata taattggttt cttctgaaaa tcttgtgttt tttccattat
     5821 atgattaagg ctaaagatat aagctcctag tgaatcataa gacgacaagt tatccaaaca
     5881 ggctagaccc gaaaacaaag tgttacttaa agcaaacaga tattctgatg caattaattc
     5941 tacatgattt gtcaaacgaa ctaaaaacga atgaattctg ttaatttctt caatctgatt
     6001 ttgaccttgt tttatctttg taagcactat agcaaaatat aagttgataa cttctaattc
     6061 atttatcaaa aacaattcct tatcataaat tgttttaaaa taatcgtcca gtacggtttc
     6121 cccgtattct ggtcgtccac ttcgaataac atctatcgtt gctctagtag cttcaaacca
     6181 tactttttcc tcattaggaa gttgctcata aaataaatcc tctatctctt ccaaacacgc
     6241 ttccttctta tcgtactcct cttctttacc atagattggt tcacgtaaaa tctggtattt
     6301 caattctaaa taagcagaag gaagagctga aaaatccggc ataaggctat aaactggaac
     6361 tcctagccgg cgagcaatat aggctaactt gggcaaactc ggttgcgaag ctccactttc
     6421 aattcgcgac agttgacgaa ctgtcagttc ttgctcatct ccacaaaaat ctggacgact
     6481 aattcctttt tctatccgaa tctctttaat tttttgacca attaacatca gacgcctcct
     6541 attctacctg cttcgaaaaa tactgtgaaa cttaaaaaat tgtaacggct acatctttcc
     6601 tactcataag atttcatagc ttcttccact tctgccaaag taaccccaaa caactctagg
     6661 cgtttgagga gttgcttgcc gttggaatag ccgattcgga gagtctctcc gaggtattct
     6721 ctacgcttac ggctgtctgc ccctgctaga aaaccaaggc gaatcaaatc gctacgacta
     6781 atgtcaaact ggctctcatg ttcaaattgt tctgtcacct gagctagagc cgttttcagg
     6841 tcttcatagc tggcatgctc aattcccaga gaacgcccct tggtcttgga cttgggaaca
     6901 gcttcatctc gcttgagaaa ggcatgctga actgttggaa tgaccgtcat gatcatgcgc
     6961 cgaatccgtt ctccattaaa atctgggtct gtaaagacaa tgactccatg acgttggtgc
     7021 aggcgctgaa tccgctctat atcctgatca ttgatggcag aacctcgagt ctcataggtc
     7081 tccacatcga aataacgctt gagattgacc gtatcatcac gcccttcaac cacgataact
     7141 tgagaaattt tctctttcat tacttgctgt ccagtccaaa aattcgttct gcatttgcag
     7201 tcgttgctac cgccagctct tctgtcgtca taccacgcaa gtcagcgata aagtcgacca
     7261 catagcgagt ataggctgtt ttattttcac gaccacgctt gggtacaggt gctaagtaag
     7321 gcgcatctgt ttccaccaaa atcttgtcca aaagtaactc tttagctgct tcttggaggt
     7381 cagttgcctt cttaaaagtc accactcctg agaaggaaat ggtcatacca agatccacaa
     7441 acttctctgc ccactcaagc gtccctgaaa atgaatgcat gataccacca cgaggaccaa
     7501 cgccctcact cttgataatc tcataggtat cttccagcgc atcacgggta tggacaacaa
     7561 aaggcaaatc caagtcctta gatagctgaa tctgacggcg aaaaacctgc tcctgcacct
     7621 ctttgggcgc tgtcatccaa tggtaatcta agccaatttc acctaaagcc acaaccttgg
     7681 gatgttttaa cttatccaac aagtaagcct caacttcctc tgtataagta ccagcttctg
     7741 taggatgcca accaatagtc gcatagagct gatcatactc atctaccaac tccaaggcac
     7801 gctcaatcgt cggtttatca aaaccaacaa tattcatctg tgtcacaccc atctcagcag
     7861 ccaaggcaat ttcttctgcc tcacgacctg caaattcttc tacattcaag tgtgtatgtg
     7921 tatcaaaaat catctcttct aacctcgttt tctatcttct attataccaa aaaagaggag
     7981 gggcacctaa tttttcggtt tcccctcctc tcttcaatag agagctattc tgctatcttt
     8041 tctatccgat attgcccatc tcctattcca cagttagaga cagaagagat tggctacatt
     8101 gatagaatat tcctattcag ctaaagtatg tgattatctt gggttctcac ttttctcacc
     8161 catcactagg attgatgact tctcaaaatc ccccttataa tctaacttta agtagagaat
     8221 agaattttct agctgttcta ttttctacgt ctagtctttc ttcctagcaa agcaaaacca
     8281 cttgttgcca ccactagacc aagactagca agtgctgcac ttgatgctga acctgtgtta
     8341 ggtaattgtt tgccagcagg tgactggtag gtaacatctt gcattgttcc tgcagttgct
     8401 tgcggtgcac ttgttgtctt aggagcagca ttaggagcca ctggtctctt aggaactgta
     8461 accatggcaa tggcttgcac tccaatcgtt tctgcttcct tcacatcttc catagttttc
     8521 gcattttcaa tctctttgag agctgcttgt ttttctgctt ccactcttgc taaaagttct
     8581 gctttttctt tatcagaaag cggtgcgcct ttgatttcat cctgcttgtc cttggctgct
     8641 ttttcaatgg catttttagc tgattctttt tcagtagcta attgcttaga agcttgtgat
     8701 tgagctgagt tttgtgaacc tgtgtgagct gatccacccg cctggctacc accgccttca
     8761 ttcgcattag catctgagct tccagtattg cctcccttat ctccacctgg tgtattttct
     8821 agcttatact ctggtgtagc ttcttgagtt acagattcac ctttcgcaga ttgttgaact
     8881 gtatcttttc ctagaatcgt cactactgaa ccatctggga atgtgattgt tgctgtacca
     8941 tctccagcta cttcaacttt atagtaaaat aaaataataa caggacaaat cgatcaggac
     9001 agtcaaatcg atttctaaca atgttttaga aacagaggtg tactattcta gtttcaatct
     9061 actataacat cgcttccgaa tccatttgca tctactgaac ttttttacaa aaataaaaat
     9121 cagtagactc gagttcctac tgatttttga tagaattcgt ttggattatg cagccaaaac
     9181 tttgcgtcct ttacgacgac gagctgccaa tacgcgacga ccatttttag ttgacatacg
     9241 gttacggaat ccgtgtttgc gcgcacgacg aagtttactt ggttgataag tacgtttcac
     9301 gatgaatacc tcctcataga ttttgtattc gtttagccgg ctataaagtg atcagttact
     9361 aaacatactt tactattcta tctgattctt tcacttttgt caatagccct aggcaaatgt
     9421 ttccagcttt ttcgaatgca agccctatct acgatactgt ttcaagaaac gagtcatctt
     9481 ttcttggatt tgggctaact catcaacacg aggaaggtaa acgatacgga agtggtctgg
     9541 ttcctgccag ttaaagcctc gaccatgaac caagagaacc ttttcctgct tcaagaaatc
     9601 aaggacaaac tgctcatcat catcgatacg gtacatattg cggtcgattt ttgggaagat
     9661 atagagcccc gccttgggtt taacggcaga caaacctgga atatcttgaa tggcattata
     9721 gatgaaattt ctttgctcgt agattcgtcc accaggaaga agcaattcat cgactgattg
     9781 gtgacccccc aaggaagttt gtacgacttg ttgggccaaa acgttagagc aaaggcgcat
     9841 attggacagc atattgagcc cttcgatata gcccttaaca tgagtcttag ggccagacaa
     9901 gaccatccaa cccacacgga aacctgctat gcggtgggat tttgacagac cattcatgct
     9961 gacacagaag acatctggtg ccaagctcgc cacaggcgta tgcacatgtc cgtccattac
    10021 catgcggtca tagatttcat ccgcaaagat aatcaaatcg ttttgacggg caatctcaat
    10081 aatctccagc aagagtttct taggataaag ggctccagtt gggttatttg gattgataag
    10141 gacgattgcc ttggtattgg aagtgatttt tgacttgata tcgtcaatat ctgggtacca
    10201 ttcaacagct tcatcacaga tatagtgaac ggcatttcct ccagctaggc tgacagccgc
    10261 cgtccataga ggatagtctg gcattggcac taagacc
//
