LOCUS       NZ_CP113114            10297 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Streptococcus pneumoniae strain NP1 chromosome, complete genome.
ACCESSION   NZ_CP113114
VERSION     NZ_CP113114.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Genome sequencing and assembly of Streptococcus pneumoniae strain
            NP1 isolated from nasopharynx
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2022) Community Medicine &SPH, Post Graduate
            Institute of Medical Education & Research, Chandigarh, Room No 529,
            Research Block B, PGIMER, Sector 12, Chandigarh, Chandigarh 160012,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: MaSuRCA v. 3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 356.0x
            Sequencing Technology :: Illumina Novoseq 600; Nanopore GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 02:39:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,409
            CDSs (total)                      :: 2,335
            Genes (coding)                    :: 2,154
            CDSs (with protein)               :: 2,154
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 86 of 181
            Pseudo Genes (incomplete)         :: 87 of 181
            Pseudo Genes (internal stop)      :: 72 of 181
            Pseudo Genes (multiple problems)  :: 53 of 181
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:47:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1429562
            Orig. end                         :: 1439859
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(925..1413)
                     /locus_tag="OTU45_RS07155"
                     /old_locus_tag="OTU45_07155"
     CDS             complement(925..1413)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /locus_tag="OTU45_RS07155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07155"
                     /product="hypothetical protein"
                     /protein_id="WP_000093676.1"
                     /transl_table=11
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYKI
                     GVQKLEELLQSPYSIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFYTK
                     DRSILDNHLDHLLEKMVNDPEKLADFIMDKSLDDAIDEAKSQITFRQEGAVKQKEMVR"
     gene            complement(1403..1702)
                     /locus_tag="OTU45_RS07160"
                     /old_locus_tag="OTU45_07160"
     CDS             complement(1403..1702)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /locus_tag="OTU45_RS07160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07160"
                     /product="hypothetical protein"
                     /protein_id="WP_000869764.1"
                     /transl_table=11
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKRDYIEFKRV
                     GIFDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            complement(2296..2928)
                     /locus_tag="OTU45_RS07165"
                     /old_locus_tag="OTU45_07165"
     CDS             complement(2296..2928)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730165.1"
                     /locus_tag="OTU45_RS07165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07165"
                     /product="putative bacteriocin export ABC transporter"
                     /protein_id="WP_000623394.1"
                     /transl_table=11
                     /translation="MISIEHLTKSFGQRTVFQDLSLQFTEGKVYALIGNSGCGKTTLLN
                     ILAKLEPYEKGSISYQGQELKQIKQHHFFKHELGYLFQNFGLLENETIATNLDLGLIGQ
                     KLTKKEKKQQEEEVLKKVGLAYLALDQKIFELSGGEAQRVALAKVILKDPPLILADELT
                     AALDPETSQEVMDLLLMLKNQERIIIIATHNPDIWNQADEVINLNQL"
     gene            complement(2930..4939)
                     /locus_tag="OTU45_RS07170"
                     /old_locus_tag="OTU45_07170"
     CDS             complement(2930..4939)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825229.1"
                     /locus_tag="OTU45_RS07170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07170"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_000825229.1"
                     /transl_table=11
                     /translation="MKRLFIHFSNLFILIFLLWIAFISPNTVIHRSLPVIGILQQEREV
                     VYEELSSSLDQLAKENNSLIARQIQKTDSKGQVKFSYDIYGEGALPNGIKKEEKEFVAK
                     ESLLTNYYILSGNLTLEKLDQKLHDLGFSKSFMNKPNPLQNFMVFFSSGSQSLALVIFI
                     ISFAALTIIQKTLEMRSAGIRYISGIRRYQLFGHSLMEDGKELFLGCIGGSVLGAILIY
                     YLQLTPFAYSLIISASIIYNTLLFILSAFLSFFFAFSIQTVHLVSLLKGKIPLKRVLFF
                     LFTCQFLAITVIGLSIHRVSIYGSIWQTYQEGSLAWSKETNWIQISLNRENVLQTTNKE
                     MQIEQIAKWSKLIESGIEKGGLLVHHQLAAFNSKGFMNDPRTGRELSITDYDPLANTLY
                     VTPNYLDIQRILVSPEEKERLNHLQAGEFGLLLPEKLKGQEEELKKRYEDYLTPSDEQG
                     KSQLPMKARVTYLPNNQKRFIYNNTPMIYQQFLTDPILVVVLPRSFGGYDNPYFSHLNS
                     YLYFDGLEKSKKLVVENGLEKNVSQYDYAASVYQQMIQSIQLENLITIAGGIFAIATSI
                     LLFNTMNFLYFEEFRRPIFLKKIAGLGFVKIHQMILLSESILLLLGSFLTFLLTQEWWI
                     ALVTLLLFITNAWFILLYRSHKEDHLLATVLKGA"
     gene            complement(5001..5297)
                     /locus_tag="OTU45_RS07175"
                     /old_locus_tag="OTU45_07175"
     CDS             complement(5001..5297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904659.1"
                     /locus_tag="OTU45_RS07175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07175"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_050199870.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 5.6e-07,
                     bitscore: 27.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTSKIILSVLTVIISCSLACPVFADWVSGGNWSYGGYHNPGNWG
                     AFSNYFHDYRWHWSSVTRASDSKANVGYASAHYTSRSFINTSFGETAYFNYGF"
     gene            complement(5551..6073)
                     /locus_tag="OTU45_RS07180"
                     /old_locus_tag="OTU45_07180"
                     /pseudo=""
     CDS             complement(5551..6073)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713386.1"
                     /locus_tag="OTU45_RS07180"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07180"
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLFLRKENEMVDKREKLMNSFNQYGFLTFKQVIDENLHYKTLLKM
                     LAEGKIDAEEKGLYRLPDIYLDEWFVLQYRFPKGIFSLETALWLHGLSLTIPFNMTMSF
                     PYGTNTKNIKEADICPIILRSHYSEGIIEIERLPGQFIKVYEVERVLVECLCPAI"
     gene            complement(6400..7035)
                     /locus_tag="OTU45_RS07185"
                     /old_locus_tag="OTU45_07185"
     CDS             complement(6400..7035)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649777.1"
                     /locus_tag="OTU45_RS07185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07185"
                     /product="hypothetical protein"
                     /protein_id="WP_000222719.1"
                     /transl_table=11
                     /translation="MTYTLEWLKTFDGEIDILNIKMNCLANTIEKNVSQYKYIYQTNME
                     NCPEIILSVPNSSIPHLLGLSREHHVNLPTNNAGSIFEGLKDDWTLESLNKADNGWFNE
                     NKFKIVGTLLLYQMLHVMECKFYTTDGILNRRVGSRFRRDNIYFVVFKLSNGISYTVEL
                     SREQGNQYFPRSLKINDTSVTDCREIDLRLINKIRFKPVKARKVKWPS"
     gene            complement(7325..7912)
                     /locus_tag="OTU45_RS07190"
                     /old_locus_tag="OTU45_07190"
     CDS             complement(7325..7912)
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /locus_tag="OTU45_RS07190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07190"
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_000821296.1"
                     /sec_met_domain="Abi (E-value: 1.6e-14, bitscore: 51.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYSSSLISF
                     LWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQGTTNQANLIGLQHQVPW
                     FSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTILAWCLYVSLG
                     MFLGLVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            complement(7915..8148)
                     /locus_tag="OTU45_RS07195"
                     /old_locus_tag="OTU45_07195"
     CDS             complement(7915..8148)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /locus_tag="OTU45_RS07195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07195"
                     /product="hypothetical protein"
                     /protein_id="WP_000840776.1"
                     /transl_table=11
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQKI
                     RHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            complement(8161..8541)
                     /locus_tag="OTU45_RS07200"
                     /old_locus_tag="OTU45_07200"
     CDS             complement(8161..8541)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /locus_tag="OTU45_RS07200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07200"
                     /product="hypothetical protein"
                     /protein_id="WP_000566525.1"
                     /transl_table=11
                     /translation="MIDLYLSKNNHRNQILLDFFQNYGIEVSCHSISEMTKDKLIEMMS
                     YSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELSIRLPLAVSNKRVYPNLNLEEA
                     RALLPRDTKQLIYMAQTHYLSN"
     gene            complement(8534..8983)
                     /locus_tag="OTU45_RS07205"
                     /old_locus_tag="OTU45_07205"
     CDS             complement(8534..8983)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /locus_tag="OTU45_RS07205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_07205"
                     /product="hypothetical protein"
                     /protein_id="WP_000645879.1"
                     /transl_table=11
                     /translation="MKALNKESILDCDELETELHDAEIKQLDEQIFLMPNYLCEFEVTF
                     LDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGADAFIDDNQNLVFVLYGQGYRAEGKE
                     GILTTQVTVKAYDEDKKSINFSDLLDSLIVSEYQMEPNLWEVSHD"
     misc_feature    9935..9954
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 19.76"
                     /tool="antismash"
     misc_feature    9938..9952
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 19.0"
                     /tool="antismash"
ORIGIN
        1 taatgagttt tgagatttca gcaaaacttg gataacgccc tcgttttttt cgctcctcca
       61 cttctttttt ttgacgtttc aacaagtttt tgtattcttt ttgacttaaa cgactttctt
      121 ctatgagctg ttctcttgtt ttgggtggat tatagaaatc gaccaagtag gaggctaaag
      181 ctcggaccaa agtcatagaa gcttcatccc aaaatggatc actacgagag ccagagcctt
      241 tggtgttatt gaaataaacc gtcagcatgc gattcaaatc attttctgtc tctatatagc
      301 gaaaaggatt gaagccatct gagttcttca tattgactaa atctaacacc tttacttggt
      361 agccatgttc taaaaagagt tttcctgttt tctcggccaa gtgatcttta ggatccacta
      421 caatattaga actattcatc tgaattagat tgggtttcac aaagcgaaat gtcttcccac
      481 ttcctgaacc tccgatcact gcaatattct tatttctatc atattggggt ggttttttat
      541 ctaataatgt caaacgaaca tcttgtgcta agatcgtatc atgtaaaaat tccttaccgt
      601 aaaagagctt cttttctttt agagttccaa aacgggcgct cccgtattct accccttctc
      661 ggtattgttt tttaccagtc tctagataga gataaaccag caacatcatc acaaagccta
      721 gtagaaaaaa agcacttgat tttccagtaa aggaaacatt ccatggcgac tgaagaactt
      781 catcttgacc ttccatcaga agatgaatcc atttatctaa tgtatttcca gtataggaat
      841 catacaaaag cgtcaaacga tgaaaaagat agcctagtaa gatacctaac agtgagaata
      901 gtatgaattt ctttccactg tacatcatct caccatctct ttctgtttga cggcaccttc
      961 ttgtctaaag gtaatttggg atttagcctc atcaattgca tcgtctaatg acttatccat
     1021 gataaaatca gctaatttct ccggatcatt aaccattttt tctaacagat ggtctaaatg
     1081 attgtctaga atcgaacggt ctttcgtgta gaaatgcaga gaatcccctt gccaagcgat
     1141 ggctaaagga atctcttctt tttctaaaaa agctttaaat ttctctatat caattggttt
     1201 gtctaaaaaa tcttttttca gattaatcgt atcaatcgaa tagggagatt gtagcaattc
     1261 ttctaatttc tgcaccccta tcttataggc ggaatcttgt gctaaagcct gacgtctaga
     1321 ccattctaga atctttaaaa gactttttac agtaaataaa agactacgct cagcatattg
     1381 aactgccatt cgttcctgtt gttcagagga catctgatgc ctccttctta acaaatagca
     1441 gctttccttc tttataacga taagctatca atttctgacg ttgcttaatc gacttgacaa
     1501 cctgcagtag atctctcgaa taaggttctc gaagagtaac ttctactgct ttcccatcaa
     1561 agataccaac acgtttaaat tcaatgtagt ctcttttgag atgtcctaag cccataccaa
     1621 aaggaaagtg atgaatcaat tggatggtac aaccaccttt atttcccatt tgtttcaaat
     1681 catacaagtt tactaccttc atgattaact cctttatcta gtttgtcgca tcgtttaagt
     1741 ctggtaacga taaactccgt gtctgttaga aaattctcac acacgtcttg tgccagtcgc
     1801 ccttcacagg gaaatactct cagtccctac ttacacaggc acgctaatca agacggagtg
     1861 gattcaattt tcaaagaaca ggtagcttta ttatagataa gagtagttga aatttttatc
     1921 acatttttgg ggttgttgga ataataaggt aagtgctaat ttctagctcc tctcacaaca
     1981 ccgtacgtac cttatatgtt ttatgaacag tagctatcat ttacattttc aaactaccat
     2041 tctatcaaac taaaagtcaa aatagacgct tctacttcga gagatactca tttaatggtt
     2101 cagcaattcg ctctacttcc gttacagaaa tttcatcact atgacttcta atgatttgtt
     2161 attaattcac tttgaggggc ttgctcatga aaccaaacag tgtaagtcgt agaagaaaaa
     2221 agcagttcaa ttgaactgct tttacggatt cagataaaat aactttaaaa tatagaagat
     2281 atctattgac tgtaactata attgatttaa atttataact tcatctgctt gattccaaat
     2341 atcgggatta tgggttgcaa taatgattat acgctcttga ttctttaaca ttaagagcaa
     2401 gtccatcacc tcttgtgagg tttctggatc cagtgctgca gttagttcat ctgccaaaat
     2461 taaaggtgga tcttttaaaa taactttagc taatgcgaca cgttgcgcct ctcccccaga
     2521 taattcaaaa atcttttgat ccagagcaag gtaagccaat cctacttttt tgagcacttc
     2581 ttcttcttgc tgcttcttct cttttttcgt taatttttgc ccaatcaatc ccaaatctaa
     2641 atttgtagcg atagtctcat tttcaagtag gccaaagttt tgaaagagat agcctaattc
     2701 atgcttaaag aagtgatgct gtttgatttg tttcaattct tgcccttgat agctgatact
     2761 tcccttttca taaggctcta actttgccaa gatattaagc aaggttgttt taccacaccc
     2821 gctatttccg atcagagcat agaccttacc ttctgtaaat tgcaagctca aatcttgaaa
     2881 gaccgttcgt tggccaaatg atttggttaa atgttctatg ctaatcatat taggctcctt
     2941 tcagtacagt ggctaacaag tggtcttctt tatgagaccg gtacaggaga ataaaccaag
     3001 cattagtaat aaatagcaag agagtgacaa gagcaatcca ccattcttga gtgagaagga
     3061 atgtcaggaa gcttcctaat agtaagagga tactttctga gagcaaaatc atttggtgaa
     3121 tctttacaaa tcccagacct gcaatcttct tcaagaagat tggtcttcta aactcttcaa
     3181 aatagaggaa attcatggtg ttaaagagta gtatggaagt agctattgca aagattcctc
     3241 ctgcaatggt aatgagattc tctaattgaa tggattggat catctgctgg tatacagacg
     3301 ccgcatagtc atattgacta acattttttt caagcccatt ctcaactact agtttcttgc
     3361 ttttttccag accatcaaag taaagataag aattaagatg agagaaataa ggattatcgt
     3421 agccaccaaa gcttctaggt agaacaacaa ccaaaatcgg gtctgtcaag aattgctgat
     3481 agatcatcgg tgtattatta tagataaatc gtttttggtt gttaggaaga taggtcaccc
     3541 gtgctttcat aggcaactga ctctttcctt gttcatcact aggagtcaga taatcttcat
     3601 agcgtttttt caactcctct tcttgccctt tcaacttttc aggaagcaag agtccaaatt
     3661 ctccagcttg caagtgattc aagcgctctt tttcctcagg ggaaactaag atcctttgga
     3721 tatcaaggta atttggtgtt acatacaggg tgttggcaag aggatcgtaa tctgtgatcg
     3781 aaagctctct ccctgttcta ggatcgttca tgaagccttt tgaattaaat gcggcaagct
     3841 gatgatggac caaaagtcca cctttttcaa tgccagactc gatcagttta gaccatttag
     3901 ctatttgttc aatttgcatt tctttgtttg tcgtttgtaa aacattttcc cgattcaaac
     3961 taatttgtat ccaatttgtc tctttagacc aagccaaact tccctcttga taagtctgcc
     4021 agatagagcc atagatgctg acacgatgaa ttgagagacc aatgacagtg attgcaagaa
     4081 attgacatgt aaagagaaaa aacaagactc tttttaatgg aattttcccc tttaaaaggc
     4141 taaccaagtg tactgtctga atactgaatg caaagaaaaa ggataggaaa gcagataaaa
     4201 taaataaaag tgtattgtaa ataatactag ctgaaatgat gagagaatag gcaaaaggag
     4261 tcaattgtaa ataataaatc agaatagctc ctaggacact tccaccaata caccctaaaa
     4321 acaattcttt accgtcttcc atgagagaat gtccaaagag ctggtatcgt cttattccag
     4381 aaatgtaacg aatccctgca cttctcattt caagtgtttt ttgaataatg gtcaatgcag
     4441 caaagctgat gataaaaata accaaggcta gagattggga acccgaacta aaaaatacca
     4501 taaaattttg caggggattg ggcttattca tgaaactttt tgaaaaacca agatcgtgga
     4561 gtttttgatc taatttttca agggttaaat tcccagatag tatatagtaa ttggtaagca
     4621 aactttcctt tgcaacgaat tctttctctt cttttttgat gccattagga agtgctcctt
     4681 ctccgtagat atcatacgaa aacttgactt gccctttaga atccgttttt tgaatctgac
     4741 gagcaatcaa actattgttt tcttttgcta actgatctaa gctagatgaa agctcttcat
     4801 aaacaacttc cctttcttgt tgaagaatcc ctattacagg taggcttcga tggatgaccg
     4861 tattaggtga aataaaggca atccaaagca gaaagattag aataaaaagg ttcgagaaat
     4921 gtataaataa acgtttcata attctactct ttctagttta ccctagagag atttcttctc
     4981 tctaggataa actttagcaa ttaaaatcca taattaaagt atgcagtttc accaaaactt
     5041 gtatttatga acgatcttga tgtgtaatga gcagaagcat aacctacatt agctttgctg
     5101 tcacttgctc gagtcacact tgaccaatgc catcgatagt cgtggaaata atttgagaat
     5161 gctccccaat tgcctggatt atgataacct ccgtaactcc agttaccacc agatacccaa
     5221 tcagcaaata caggacacgc taagctacaa ctaataatta cagttaagac agaaaggata
     5281 attttactcg ttttcatgac tgttcacctt ccataattag aattaaaagc tttatttaac
     5341 aataaaatta tgaattgctc aaaatatagt ttccgttttt tctcctttcc agagcaaccc
     5401 ttttcgcctg cattataaca cttacaacat ataatttcaa ctaattatat aatagatttt
     5461 tgaatattta ataatcaaaa caatatagct gtttattcat aagctatcaa ccaaattcta
     5521 aaactattaa agtttttgta ttaaaaaaca ttaagatagc acctccgtat aagattgtaa
     5581 cttatcagtt actttaaata gctgggcata gacattcaac caaaactcgt tcaacttcat
     5641 aaactttaat aaattgacca ggaaggcgct ctatttcaat aattccttca ctatagtgag
     5701 agcgtaaaat aataggacat atatctgctt ccttaatgtt tttggtattc gtaccataag
     5761 gaaaactcat cgtcatgtta aaagggatag tcaaagataa accatgtaac caaagtgctg
     5821 tctccaaaga aaagattccc tttggaaatc gatactgaag gacaaaccac tcatctaaat
     5881 aaatatcagg taagcgatat aacccttttt cttcagcatc gatttttcct tctgcaagca
     5941 ttttcaataa ggttttgtag tgtaaatttt catctattac ttgtttaaaa gttaaaaaac
     6001 catactgatt gaaagagttc atcagttttt ctcttttatc aaccatttca ttctcctttc
     6061 ttagaaataa aatgctaaca tcttatatat ctgttagcat tttatttctt taaaatcaat
     6121 tcatttagtt ttttatttca aactgaatct ataagtaagc tagaccacct attataaaga
     6181 agaagtaagc catggactaa tagtttccac tccaaatgaa gccctagaaa catttctagt
     6241 aaaaacatac aaacagcaag ataaaatgct tgtcaaagaa actctgatat ctaattttct
     6301 gattaacttt cataaaattt ctcgatacag aaattcttcg acaatactca catttgttaa
     6361 gaaaagaata gtgatgtaat catgatttca atttttaaat catgatggcc atttcacctt
     6421 tcttgcctta actggcttaa atctaatctt attaatgagt ctaagatcta tttctctaca
     6481 atctgtaacg gaggtatcat tgattttaag gcttcttgga aagtattgat taccttgttc
     6541 acgtgatagt tctactgtat aactaatacc attacttaat ttaaagacta caaaataaat
     6601 attatctctt ctaaatcggc taccaactct tctattcaaa atgccatcag ttgtatagaa
     6661 tttacactcc attacatgta acatttgata aagcaacaag gttccaacta ttttgaattt
     6721 attttcgttg aaccaaccat tatcagcttt attcaggctt tccaatgtcc aatcatcttt
     6781 taatccttca aaaatactcc ctgcattatt agttggtaaa ttaacatgat gttctctaga
     6841 taatcctaat agatgaggaa tggaggagtt tgggactgat agaataattt caggacagtt
     6901 ctccatattt gtctgataaa tatatttgta ctgagaaaca tttttctcaa tagtattggc
     6961 caagcaattc atcttaatat tgagaatatc aatctctcca tcaaaagtct ttagccattc
     7021 caaagtataa gtcatttgaa ttttctccta aaaaagagaa ggaagctcct gcttccttct
     7081 ctcccacgta ttgagtttca cctcacgcta gatatgccct atctattcct caagggtagg
     7141 cagtcctgct attctttttc tcaacaaaca gtctgaactg agagcggagc ccacaggcgg
     7201 gtgcagacca tatccactag cccacaagta attatatcat ttaattatct tcattatacg
     7261 caattagtcc atatttgtca acaattacat tcattatgaa tatctaaaaa tcaggtcacc
     7321 tattttaaaa aagcagcaaa ctataaacta gtaggttcca caccaaatgt agccccatac
     7381 tgccccataa gtccgatttg tagcgtacaa gccctaaaaa catcccaagt gaaacatata
     7441 aacaccaagc tagaatggtt cctggatgat gtgctaaggc aaataaaaca cttgtcaaag
     7501 caactcgaat atctaatttt ctaaccaagt tccataaaat ttctcgatac agaaattctt
     7561 caaccatact cgcattgatt aagaacaata aaaatgaaaa ccaagggact tgatgttgaa
     7621 ggccaattaa gtttgcttga ttcgtagttc cttgagcatg gattaggcta aaacatagac
     7681 ttataatcag taggctaacg aatccaatac caagccattt catcctagat ttcatattga
     7741 ccttatgagc ttgtttgcgt tgaccataca tccataaaaa agaaatgagt gacgaactat
     7801 agagaatctg tagtatagtt aactcaccga tacaaagaaa tttcaataag tatagagata
     7861 ccaataggac atttacttgt tggaatatat aaactggaat tattcttttc atagttacct
     7921 ccgaaataaa tcttcataat ctaaatctaa tacctgcaca atcctttcta cccatggact
     7981 ttgaggcatt cgttgttcca tcttgtagtg acgaatcttt tgatacaaac gattcaattc
     8041 acttggatag tgaaactctc ccgcaaacat ttttctggtt aactcaatcc agctaatatt
     8101 tctttcagct aaaataatgg acaagttctc ccaaaatcgt tcagccatat tgcttctcct
     8161 ttagttagat aaataatgtg tttgtgccat gtaaatcaat tgtttcgtat ctcttggcaa
     8221 tagagctcta gcctcttcca aattcagatt tggataaact ctcttatttg aaactgcaag
     8281 aggaagtctg atgcttagtt caggattttt taatatcatt tcgatgaaat ccgttaatct
     8341 tagattgtca cggttcttaa atcgtaataa attaggagat aaaaactcaa aacaatctga
     8401 agaatagctc atcatctcaa ttaatttgtc ctttgtcatt tcagaaatcg aatgacaaga
     8461 tacctctatt ccgtagtttt gaaagaaatc taaaagaatt tgatttctat ggttattttt
     8521 acttagatag agatcaatca tgggagacct cccaaagatt cggttccatt tgatattctg
     8581 agacgattaa ggaatctaat agatctgaga agttaattga tttcttgtct tcatcatagg
     8641 cttttacagt tacttgggtt gtaagtattc cctcttttcc ctcggctcga tagccttgtc
     8701 catataaaac aaaaacaaga ttctgattat catctataaa ggcatcagct ccgttcttta
     8761 tatcctgact ttcgaggaat tccataacgt tttgaagata ggattcataa aatagtggat
     8821 agttatgttt tttatggtaa tcatctaaaa atgtcacttc aaactcacat agataattgg
     8881 gcattaaaaa tatttgttca tccagctgtt tgatttctgc atcatgtaat tctgtttcta
     8941 attcgtcaca atctagtatt gactctttat ttaatgcttt tatctttttc ctctatttct
     9001 tttaatttct ttgcgattgc ggcaatcaca ggaacggtta cactattacc aacttgttta
     9061 tagagctgac tattaataga gacttttcta gcagcttcaa aagcctaatc aggaaagcca
     9121 tgcaatcgaa aacactcttt aggagtgatt cgtcgtattc tcaaacggta aaattgtcca
     9181 tctattaaaa caccagctac ttggtaaact tgtttatctt ctccttcata gctagccact
     9241 actactccca tttgaccact agttgttaac gtattagcta taccttttcc aactctacca
     9301 cgacgatact gagaacttgg tctttctaaa ttgattgaat ccccaatctc tgcttgagca
     9361 tatccttttt tcgttgcttc ccgtactttt agaaattgga ttggttctgg aattagtatt
     9421 ttggggattt tatctcctcc ttgcatcgta gtcagtgttg gagataagcc ctcacttcca
     9481 tagacacgac ctgtctcctt aaagctagtc ggtaaatctc caacaacgac aatgccataa
     9541 cgatcctgag tatttaaagt aaacatcggc tcttgatttt ccttaaagcg tctcccattt
     9601 tgtctcttgt ctaatctatc tggtgtcata caaggaatcg caactttaaa tccttctcct
     9661 ttaccacgaa ctaaggttgg cgcaagacct tctgaataat agactttacc gctcattcca
     9721 cttcttgatg gattcaaatt tcctagtgct ttcaaagtct cagagttagt tgcttgacct
     9781 tctcgtctga aaggaaataa gagtctggta cctttctttc tagaatgtcc gataataaac
     9841 accctctctc tgttttgggg aacgccaaaa tccttactgt taagcacctg ccactcaaca
     9901 tcaaacccca actcatcaag tgtggtaagg attgtggtga acgtccgtcc cttatcgtga
     9961 ttgagtaagc ctttaacatt ttcaagaaaa agaaaacgtg gttggatttg tttggccgcc
    10021 cgagcaattt cgaagaacaa agttcctcta atatcttcaa atcccaatcg tcttcctgcg
    10081 attgaaaatg cttgacaagg gaatccccca cagatgatat cgactttccc tctaagtttt
    10141 ttaaattcgt catctgaaac atctcgtatg tcatgaaatt cgatttctcc ttccgtttga
    10201 aaaatggatt tataagattc tctagcaaat ttatcaatct cacaaaatcc aatacactcg
    10261 tgtcctacac tttccattcc aagtcgaaaa ccaccta
//
