LOCUS       NZ_CP113114            20876 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Streptococcus pneumoniae strain NP1 chromosome, complete genome.
ACCESSION   NZ_CP113114
VERSION     NZ_CP113114.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Genome sequencing and assembly of Streptococcus pneumoniae strain
            NP1 isolated from nasopharynx
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2022) Community Medicine &SPH, Post Graduate
            Institute of Medical Education & Research, Chandigarh, Room No 529,
            Research Block B, PGIMER, Sector 12, Chandigarh, Chandigarh 160012,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: MaSuRCA v. 3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 356.0x
            Sequencing Technology :: Illumina Novoseq 600; Nanopore GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 02:39:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,409
            CDSs (total)                      :: 2,335
            Genes (coding)                    :: 2,154
            CDSs (with protein)               :: 2,154
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 86 of 181
            Pseudo Genes (incomplete)         :: 87 of 181
            Pseudo Genes (internal stop)      :: 72 of 181
            Pseudo Genes (multiple problems)  :: 53 of 181
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:47:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1220100
            Orig. end                         :: 1240976
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20876
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="5"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20876
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20876
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10876](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10876)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    90..103
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.2"
                     /tool="antismash"
     gene            complement(737..1252)
                     /gene="lacB"
                     /locus_tag="OTU45_RS06065"
                     /old_locus_tag="OTU45_06065"
     CDS             complement(737..1252)
                     /codon_start=1
                     /gene="lacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /locus_tag="OTU45_RS06065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06065"
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="WP_001216918.1"
                     /transl_table=11
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPIF
                     GKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNANVI
                     GFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEFLEK
                     WDRGEYHD"
     misc_feature    1218..1233
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            complement(1283..1708)
                     /gene="lacA"
                     /locus_tag="OTU45_RS06070"
                     /old_locus_tag="OTU45_06070"
     CDS             complement(1283..1708)
                     /EC_number="5.3.1.26"
                     /GO_function="GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /gene="lacA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /locus_tag="OTU45_RS06070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06070"
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="WP_000029272.1"
                     /transl_table=11
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTLA
                     VAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMITMG
                     AQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            complement(1865..>2362)
                     /locus_tag="OTU45_RS06075"
                     /old_locus_tag="OTU45_06075"
                     /pseudo=""
     CDS             complement(1865..>2362)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234238.1"
                     /locus_tag="OTU45_RS06075"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06075"
                     /product="aldose 1-epimerase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="NKETSKVRPYFIGGHPGFNCPLFDDEVYEDYYLEFEKEETCSVPR
                     PFPETGMLDFQDRSPWLEGQKEIDLSYALFSKDAVTLDELQSRTIALRSLKHDRGLKVH
                     FAEFPNLIIWSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIE
                     VL"
     gene            complement(2378..3180)
                     /locus_tag="OTU45_RS06080"
                     /old_locus_tag="OTU45_06080"
                     /pseudo=""
     CDS             complement(2378..3180)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /locus_tag="OTU45_RS06080"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="OTU45_06080"
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGGR
                     KPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRS"
     gene            complement(3188..3490)
                     /locus_tag="OTU45_RS06085"
                     /old_locus_tag="OTU45_06085"
     CDS             complement(3188..3490)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /locus_tag="OTU45_RS06085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06085"
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_000590566.1"
                     /transl_table=11
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVNE
                     DLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIRLPAKQNMV"
     gene            complement(3528..4004)
                     /locus_tag="OTU45_RS06090"
                     /old_locus_tag="OTU45_06090"
     CDS             complement(3528..4004)
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /locus_tag="OTU45_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06090"
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="WP_000242204.1"
                     /transl_table=11
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREAL
                     ITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEV
                     EVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            complement(4463..6286)
                     /gene="lepA"
                     /locus_tag="OTU45_RS06095"
                     /old_locus_tag="OTU45_06095"
     CDS             complement(4463..6286)
                     /EC_number="3.6.5.n1"
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="lepA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /locus_tag="OTU45_RS06095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06095"
                     /product="translation elongation factor 4"
                     /protein_id="WP_001047204.1"
                     /transl_table=11
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSREM
                     QAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLAAC
                     EGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVIGLD
                     ASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQVRVM
                     DGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQDTRVGD
                     TVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQFEPETS
                     QALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMDVSNPSEF
                     PDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVNVIYQIPLA
                     EIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFIVHKDFAYER
                     GKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGGDVSRKRKLLE
                     KQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(6365..7093)
                     /locus_tag="OTU45_RS06100"
                     /old_locus_tag="OTU45_06100"
     CDS             complement(6365..7093)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /locus_tag="OTU45_RS06100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06100"
                     /product="metallophosphoesterase family protein"
                     /protein_id="WP_000133343.1"
                     /transl_table=11
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSHR
                     VLEMVKDLVDNQGAICLSGNHEYMFLTWLDYPEESYDHYRRNGGDTTINSILGRPLDAP
                     VDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKVWLR
                     KPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLHGIVF
                     TDQGMTEHHFIENDGFVAED"
     gene            complement(7095..8762)
                     /gene="recN"
                     /locus_tag="OTU45_RS06105"
                     /old_locus_tag="OTU45_06105"
     CDS             complement(7095..8762)
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /locus_tag="OTU45_RS06105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06105"
                     /product="DNA repair protein RecN"
                     /protein_id="WP_138022600.1"
                     /transl_table=11
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNMM
                     LGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGRSI
                     SRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKETYQT
                     SFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDKLLNH
                     KNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYYVLEDI
                     SKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEYNLLTGN
                     NLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKAQFQVRFS
                     KGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKEGKTSIVFD
                     EVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKISNDHSTVSTV
                     RLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            complement(8769..9200)
                     /locus_tag="OTU45_RS06110"
                     /old_locus_tag="OTU45_06110"
     CDS             complement(8769..9200)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /locus_tag="OTU45_RS06110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06110"
                     /product="arginine repressor"
                     /protein_id="WP_050089280.1"
                     /transl_table=11
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRDI
                     KELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVKAQ
                     LIETFADKIFSCLTDDSSILVIARSGSQAEEIFEQVKNW"
     gene            complement(9193..10008)
                     /locus_tag="OTU45_RS06115"
                     /old_locus_tag="OTU45_06115"
     CDS             complement(9193..10008)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /locus_tag="OTU45_RS06115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06115"
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_001041069.1"
                     /transl_table=11
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPGE
                     KIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQNS
                     AKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISLNLI
                     LPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEVGFSV
                     LGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            complement(10001..10876)
                     /locus_tag="OTU45_RS06120"
                     /old_locus_tag="OTU45_06120"
     CDS             complement(10001..10876)
                     /EC_number="2.5.1.10"
                     /GO_function="GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /locus_tag="OTU45_RS06120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06120"
                     /product="farnesyl diphosphate synthase"
                     /protein_id="WP_000743830.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.5e-55, bitscore:
                     185.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRPF
                     LLLEVLESLQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFGEA
                     MAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQHLS
                     LEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVLDVTA
                     SFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFETESIV
                     SVVESLRING"
     gene            complement(10873..11085)
                     /locus_tag="OTU45_RS06125"
                     /old_locus_tag="OTU45_06125"
     CDS             complement(10873..11085)
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /locus_tag="OTU45_RS06125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06125"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_000043230.1"
                     /transl_table=11
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKEL
                     QATLDKAEKTLVKVMQEDGTESDFE"
     gene            complement(11063..12403)
                     /gene="xseA"
                     /locus_tag="OTU45_RS06130"
                     /old_locus_tag="OTU45_06130"
     CDS             complement(11063..12403)
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /locus_tag="OTU45_RS06130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06130"
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_000417461.1"
                     /transl_table=11
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQYF
                     SLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDGVG
                     ALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRFPGV
                     DILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVVRAIF
                     ESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEKRMATA
                     VRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDNKQLVQA
                     RTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLMLDTSRIV
                     ARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            complement(12531..13169)
                     /gene="udk"
                     /locus_tag="OTU45_RS06135"
                     /old_locus_tag="OTU45_06135"
     CDS             complement(12531..13169)
                     /EC_number="2.7.1.48"
                     /GO_function="GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="udk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /locus_tag="OTU45_RS06135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06135"
                     /product="uridine kinase"
                     /protein_id="WP_001181378.1"
                     /transl_table=11
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYKD
                     QSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQEP
                     QDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQYLG
                     VVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     misc_feature    13209..13223
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.39"
                     /tool="antismash"
     gene            complement(13277..13579)
                     /locus_tag="OTU45_RS06140"
                     /old_locus_tag="OTU45_06140"
     CDS             complement(13277..13579)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /locus_tag="OTU45_RS06140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06140"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000985089.1"
                     /transl_table=11
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSV
                     MANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            complement(13820..14698)
                     /gene="truB"
                     /locus_tag="OTU45_RS06145"
                     /old_locus_tag="OTU45_06145"
     CDS             complement(13820..14698)
                     /EC_number="5.4.99.25"
                     /GO_function="GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="truB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /locus_tag="OTU45_RS06145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06145"
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="WP_001013245.1"
                     /transl_table=11
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVL
                     PIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDEAI
                     ASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDGQLA
                     RFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAEKVEA
                     GQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLAILEKR
                     GNLYKPRKVFS"
     misc_feature    complement(14711..14720)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.22"
                     /tool="antismash"
     gene            complement(14799..17983)
                     /locus_tag="OTU45_RS12065"
                     /pseudo=""
     CDS             complement(14799..17983)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512660.1"
                     /locus_tag="OTU45_RS12065"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="SIR2 family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEDDLNYENLMDDVTEAIKKFNLVIFIGAGVSIAQGYPNWNNYIE
                     HLIKYWQGQVLSVSGEKRLGREHHVVFDLISKSSISNKRKVDLVNYELKKVFGEDFEKR
                     RLDFEKGYFKNLLPYSIVNQTVESLASLNAIFITSNYDYEIENHIKRLKNAVVTINDLN
                     EFTKNKNGKLQFGDVLHIHGTPDCDVKYFVSSSADYSKTYLKNRENFENLVTWFKETKP
                     TVLFIGAGLEEDEILSLLCKDSKNYALMKSENTGNQRVDEHYRGVVEGFFSSENHTQII
                     WYGDEFEKLPLFVKKLVADINEKLGTHDFYNQWNNLLNPSINQEEYNKNLDSISNDFKY
                     LSSVLDKVIENDNNQLDQLMLNALLRSETLTVIKKILFLHFGSLLLRILKS"
     gene            complement(17995..19269)
                     /locus_tag="OTU45_RS06160"
                     /old_locus_tag="OTU45_06160"
     CDS             complement(17995..19269)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /locus_tag="OTU45_RS06160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06160"
                     /product="DUF2130 domain-containing protein"
                     /protein_id="WP_001002649.1"
                     /transl_table=11
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTTEFDKELHDRMRQELA
                     LAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEVQA
                     LENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQLKAA
                     SEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGSKGDF
                     IFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLVTMLEA
                     DNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVREQNIDIT
                     HFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQLRLANNKL
                     EDVSVKKLTRKNPTMKAKFEALKGE"
     gene            complement(19365..20624)
                     /locus_tag="OTU45_RS06165"
                     /old_locus_tag="OTU45_06165"
                     /pseudo=""
     CDS             complement(19365..20624)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /locus_tag="OTU45_RS06165"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_06165"
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
ORIGIN
        1 tctaggaata tctaccttgt agaaagtgtc accatgtttg gcaaaagtac cgttggcacc
       61 aagtgaaacg ataatccatt caatccctgc aaacaaaggt tcttgaagta cttcttttaa
      121 ttcatccaaa tcctcagaaa cttctcttcc aagaagctga gacaattctt cattatttgg
      181 tttgatgact gttggtttat ggggtgattc aagaacagcc tgaagtgctg cacctgagca
      241 gtccaagact acaggcttgc cagcttgatt agcaagttct accaagctcg catagtaatc
      301 aactggaagg ccagctggca gactacctga gatggctact acttcaactg actccaagag
      361 ttttttgaaa tgttccaaaa actcttgacc ttcctgttcc aatacttcag gacctttttc
      421 aagaacttct gtttggttgt ctccgtggag aatagcgata cagttacgag tttctccctt
      481 aattgagaag aaatctttct ttacttgatt atcgatatgt tcaaccaaaa actcaccaag
      541 tttgccaccc actaaaccag tagcaagaac agaatcgcca aattctgaaa gtactcgggt
      601 aacattgagt cccttaccac cagccgtttt ggttacatcc accacacgat tgacagtatc
      661 aatcttcaac tcatccaagg gataggaaat atcgatggat gggttcattg tgactgttaa
      721 aatcataggt cacctcttag tcgtggtatt ctccacgatc ccatttctca aggaattctg
      781 taaagaagtt tgcgtctgtt tgttgagcat tgtgactttc aacatgttca attttcgcaa
      841 tcaatttttt gttttcttca gttggtttgt attcagcatg gatgaaagct tcgatgatat
      901 cacacataag caattcacca gtaattttac caccaaaacc aataacgtta gcgttcaatt
      961 gttctttagc ataaagggct gttgtcatat cacgaaccaa ggcagaacga acacctggaa
     1021 ctttatttac agcgttgttg ataccaacac cagtaccaca gatacatact ccaagatcag
     1081 cttgaccgct agttacagct tcccctactt ttttaccaaa gattgggtag tgagtacgtg
     1141 tatggtcata ggtaccaaag tcaatgactt catatccttt tgatttcaaa aattctgaaa
     1201 ccgccatttt ttcatctgtt acgatgtggt cacatccaat tgcaattctc atttttaact
     1261 tacctttctt actataatct aattagccca ttttgttcaa catgtcaacc cggatttggt
     1321 gacgaccacc gtcgtattta ccattaacaa atcctttagc gatgttttta gccaattcat
     1381 caccaacaag ttgtgcaccc atagtgatca tacgtgagtt gttgtggcca cgagtcatat
     1441 aagctgaacg ttcatcagaa acttctgcag caaccattcc tttgatctta gttgcaacca
     1501 taaatggacc agctccataa gcatcaatca cgataccaag gttttgttct tctttgttta
     1561 cttctgcagc aacagcgaga gtcacatcaa caaagtcttg accttcagct gtaacatcca
     1621 caaggtggaa gttttctttt tctaagaagt ctttcacaac ttctttcaat ctcaaacctg
     1681 cagcatctgc accgataaca atagacatat tgtatactcc tttttatttt ttctatgctg
     1741 gattccagca gtgtattggc tgtctttaaa aaacagtcaa ttagtcctca caaacataat
     1801 atactactat tgtttgaaga agtcaacagt ttgtgtttgt ttttgttatt tattttttac
     1861 ttttttataa aacttctatc tcaaacccta attcttcaac ctgattggct tctaagagac
     1921 aaacattctt cttatcttct aaatgatctc cttcttcaag gaatgttgac aagccagacc
     1981 atggttcaaa ggtaatgaaa ggtcccttgt tcaaagttga ccagatgatg aggtttggaa
     2041 attctgcaaa atgcactttc aatcccctat catgtttaag agaacgaagg gcaattgttc
     2101 gagattgcaa ttcatccaaa gtcactgcat ctttgctgaa aagagcataa ctaagatcta
     2161 tttctttttg tccctctagc catggacttc tatcttggaa atccaacata cctgtttctg
     2221 ggaaaggacg tggaacagag caagtctctt ctttctcaaa ctctagataa taatcttcat
     2281 agacttcatc atcaaataga ggacaattaa atcctggatg tccaccaata aagtaaggcc
     2341 tgaccttgct agtttcttta ttagacttcc tgcaaaacta gaatcctagt tcatgattga
     2401 taatgccagc aatcaaattc attcgtaatc cgaagcgttt acgatgattt cgataggttg
     2461 ttgaaatcat tttaaacgtt tttactttgg caaagatgtt ctcaaccttg cttctctcct
     2521 tagatagcgc atggttatag gttttatctt caactgttag cggcttgagt ttgctggatt
     2581 tacgtggagt ttgtacttga ggatatatct tcatgagccc ttgataacca ctgtcagcca
     2641 agattttacc agcttgtccg atatttctgc gactcatttt gaacaacttc atatcatgac
     2701 aatagttcac agtgatatcc aaagaaacaa tctcccttga cttgtgacaa tcgcttgaga
     2761 cttcatagcg tggcatttct ttttaccaga atcattcgct aattcttttt tagggcgatt
     2821 gatttgtact tccgtcgcat caatcattac cgtgtcctca gaactgagag gagttcttga
     2881 aatcgtaaaa ccactttgaa caagagttac ttcaacccat tagctccgac ggagtaagtt
     2941 gctttcgtgg ataccaaaat cagccgcaat ttcttcataa gttcgatatt ctcgcacata
     3001 ttgaagagtg gccataagaa ggtcttctag gcttaattta ggttttcgtc caccttttgc
     3061 gtgtttaagt tgataagctg tttttaatac agctaacatc tcttcaaaag tcgtgcgctg
     3121 aacaccaaca agacgcttaa atcgtgcatc agttagttgt ttacttgctt catcattcat
     3181 agaactacta taccatgttt tgtttcgcag gaagtctaat attgtcaaat actggagcac
     3241 tcattgctgg gatacggaat aagattggcc cagcttcgat aactgggata cctggttcaa
     3301 aaccaaggtc tgttgcagcg attggtgtaa agatatcgta acctttcata aggtcttcgt
     3361 ttacatcttt caccatgact gcatcacagt gaacatcata accacggttt gaaagttctt
     3421 cttctagagc acttttaatt tggtgacttg agttaacacc tgcaccgcag gcagcaagaa
     3481 ttttaatcat ttggatttcc tccgatttta ttttttaata gacaagatta agcggttgct
     3541 tcagcaatgt aagcataaag tttttctggt tcggaaattt ttgataaatc ttcaagatgt
     3601 ccatttcctg tgaaaaagtc catcaactga gccagaatat ttgtttgact tgaacttgaa
     3661 ttgttaataa taaagaagag tagggatact tctacttcct tatcaggagc tatcatattg
     3721 tgaaaagtta ctggtttttc taatcgaaca accaccactt tctcagctag attatgaaca
     3781 atatctgtat gaggaatcgc tacatttggc aagtcctttc ctagaaattc catatctaaa
     3841 ccagttggaa atgacttttc acgcgtgatc aaggcttcac gataagttgg agtgacaatt
     3901 tctcgttctt ccaataaagt tgcaacctga tcaaagagtt gttcttgatt atccgcttct
     3961 aagcaaaaca caaggttttt gtcaaagaaa taatctaata ccataaggtt ttcccttctt
     4021 tccattaact ttatgccata agtataacac tatatgaaac cgttgtcaat tactttttgt
     4081 tcttttttgt ctcttttttt atatttttgt tttgtttata gtttgttata taaaaataaa
     4141 cacacaaaca aatactccaa gcattttttc tgttctaata ctcaatgaaa atcaaagagt
     4201 aaactaggaa gctagccgca ggttactcaa aacacagttt tgaggttgta gatgaaactg
     4261 acgaagtcag ctcaaaacat ggttttgagg ttgtagatga aactgacgaa gtcagtaacc
     4321 atacatacgg taaggcgacg atgacgtggt ttgaagagat tttcgaagag tataaaaact
     4381 aaaaaagcag accatctaag cctgctttac tattgattct tatataaatt tcctgtgaac
     4441 aaggaaaggc atttctgata acttattctt catccatact caagacgctg aggaaggctt
     4501 cttgcggaac ttcaactgat ccgatggatt tcatgcgttt cttaccagct ttttgttttt
     4561 caaggagttt acgcttacga gaaacgtcac caccataaca tttagcaagt acgttcttac
     4621 gaagggcctt gatatcagta cgagcgacaa tcttgtgtcc aatagccgct tggattggaa
     4681 cttcaaattg ttggcgaggg atgattttct tgagtttatc aacgatgagt ttcccacgtt
     4741 cgtaggcaaa gtccttgtga acgataaagc tgagagcatc caccttatct ccattgagaa
     4801 ggatatccat tttcaccagc ttagatgggc gatattctga caattcgtag tcaaagcttg
     4861 cataaccacg tgttgaagac ttaagtttat caaagaagtc aaagacaatt tcagcaagag
     4921 gaatttgata gataacattg acacggttat catcaatata gtccatagtc acaaagtccc
     4981 cacgcttacg ctgagctagc tccattactg ctccgacgaa ctcctgtggt accatgattt
     5041 gcgccttgac ataaggctct tcaatggtcg caatcttagt tgggtctgga aactcagatg
     5101 ggttagacac atccatagac tcaccgtcgg tcaaattaac tttgtaaata acagacggag
     5161 ctgtcatgat gaggtcaata ttgaactcac gctctaaacg ttcctggata acatccatat
     5221 ggagaagtcc aagaaatcca caacggaaac caaatccaag tgcctgagat gtttctggtt
     5281 caaactgaag actagcatca ttcagttgca atttttcaag cgcttcacgc aggtcattgt
     5341 acttgtttga ttcgattggg tagagacccg caaagaccat aggattcatc tgcttataac
     5401 catgtaatgg ttctgccgca ggattggttg ccaaggtaac ggtatcaccc acacgagtat
     5461 cctgaaccgt cttgatagac gccgcaatgt aaccaacatc accagtcgca aggaaatcac
     5521 gaccaaccgc ttttggtgta aaaataccga cttcggccac atcaaaggtc ttactattgc
     5581 tcatgagctg aatcttatca ccaggtttga ccactccgtc catgacacgc acttgtagga
     5641 taaccccacg gtaagcatcg taaacagagt cgaaaatcaa ggccttaagt ggcgccgtca
     5701 catcacccgt tggtgctggt actttttcta caatttgctc gaggatttct tcaatcccaa
     5761 taccagcctt ggcagaagcc aaaactgctt cactggcatc caaaccaatc acatcttcaa
     5821 tctctgtacg cacgcgctcc ggatctgcag ccggcaggtc aattttatta atgataggca
     5881 tgatttccaa atcattatcc aaagctagat aaacgttggc aagagtttga gcctcaattc
     5941 cttgagccgc atcgaccacc aaaatagcac cctcacaggc agctagcgaa cgtgaaactt
     6001 cataggtaaa gtcaacgtgc cctggtgtgt caatcaagtg gaaaatataa gtttccccat
     6061 cttttgcagt gtaattcaac tcgatggcat tcaacttaat agtaattcca cgttcccgct
     6121 ctagatccat gctatccaaa agctgggcct gcatttcacg acttgaaacc gtctctgttt
     6181 tttccaaaat gcggtctgct agagttgatt ttccgtggtc aatatgggcg ataatagaga
     6241 agttacggat cttctcctgt cgttttttca attcttctaa gttcatgatt ctcttccttt
     6301 cagggtatct atttattata aattgttttt gatattttga caagaccata ccctgctagg
     6361 agtactaatc ttcagcgaca aagccgtcat tttcgataaa gtggtgttct gtcattcctt
     6421 ggtctgtaaa gacaatcccg tgaaggacac caccataaac agctcctcca tccattccaa
     6481 tcttgccatc ttctgtagtc caaagctcag atgtaccgcg ttcttgctgt aacaaaccat
     6541 agaccggtgt atgaccgaag acaatggttt ttccagtatg attttcagct ccgtggaatg
     6601 gttttctaag ccatactttt ttataatctg ttgtttcatg ccagtcgtcc aaggtcaaat
     6661 caatacctgc gtgaacaaag atatacttgt ctgtctctac tacaaatggc atttgacgaa
     6721 tgaattcgac caagtctgcc gcttcagcgg caacccgctt ggcatcttct actccatcaa
     6781 ctggtgcatc caagggacga cctaggatag agttaatggt tgtatctcca ccattgcgac
     6841 gataatggtc ataactttct tctgggtaat ctagccaagt caaaaacata tactcgtggt
     6901 ttccagacaa acagatagcc ccttgattgt ccaccaagtc cttgaccatt tcaagaacac
     6961 ggtgactatc ctcacctctg tcaatcaaat cacctagaaa gagcaactgg gtctgaccat
     7021 cccaggtttt aagaaggtct tccagcatcc cagcttttcc gtgaacatct ccaattacat
     7081 aataatctgt catcttattt ctccctgttt ctcaacaatt ctctggcttg cgtcagggct
     7141 gcttctgtca catcatcacc tgccaacatc ttggcaactt cctccactcg ctcttcgact
     7201 gtcaagagac gaacagtcga aaccgttgaa tggtcattac taatcttctc aataaagaat
     7261 tgataatctg caatcgcaat tacttgtggc aaatgggaga tagccaaaac ctgaccatac
     7321 tgaccaattt tatgaatttt ctgagcaata gcttgagcaa cacgacctga aactcccgta
     7381 tccacttcat caaagacaat gctagtcttg ccttctttac gtgaaaaggc agacttaatg
     7441 gctaacatga gacgagataa ttcccctcca gaagcaacct taaccaaggg tttaaagtct
     7501 tctccagggt tggttgaaat ataaaactca accatttcat ttccctcacg actgaatttt
     7561 cccttactaa aacgaacctg aaactgggct ttttccatat aaagatcttg cagttcttgt
     7621 ttaatctcag cttcgagttg ctgagccaaa ttatgacgag cagaagcaag ttgacctgcc
     7681 aaattgacaa gattgacttc caacttctta agctctgctt ccatgtcctc agacgaaaga
     7741 ttattgcctg tcaagagatt gtattcttcc gtaatcttgg caaaataaag caaaacatca
     7801 tcaacagtcc caccatactt acgagtaata gtatgaagga ggtccaaacg attctcaacc
     7861 tgcatgaggc gattgccatc aaaatcaagg tcctcaatga tagcttccaa acgtttgcta
     7921 atgtcttcta aaacatagta ggtctcagac agagagcttg aaatttcacg gtattcaggg
     7981 tcatactctt cgacactttc catgtcattc atggctgaac gaacattggc cagacttgaa
     8041 aaatcttcat tgtccaacat actgtaggca ttggtcagtg tatccgcaat atttttgtgg
     8101 ttgaggagtt tatctcgctc ttgattgaga gccaagtctt ctccagcctg caagtttgct
     8161 gcctcaatct ctgccatttg aaattccaac atttcgatac gtgccttgtg ttcctgttgg
     8221 tttttcttga cttccagaac ctgcttgcgc attttccgat aggcatcaaa actcgtttga
     8281 taggtttctt tcaagtccca aaaagcggca tcaccaaatt catccaacat ctggatatgc
     8341 agttggggac gcattaactc ctcatggtca tgctgaccat gaatatctac aagatgttgc
     8401 ccaatagttc gcaaaacaga cagattaacc atctgaccat ttacacggct gatactacga
     8461 ccattttgca agatttcccg acggatgata atttcatcac ctaattctaa accttgctca
     8521 tcaaaaattt cctgtaaaag acgactattc tcaactgaga aaagcccttc aatctctgcc
     8581 tttggtgcac catgacgaat aacatctgtc gtcgcacgag ctcccaacat catattcatg
     8641 gcatcaatga taatcgactt ccctgcaccc gtttcaccag tcaggacagt catccccttt
     8701 tcaaaattga gggaaatagc ctcaataatg gcaaagtttt ttatcgaaat ttcaagtaac
     8761 atatagacct accaattttt tacttgttca aagatttcct ctgcttgact tccacttctg
     8821 gcaatgacta aaatcgagct atcatcagtc aaacagctaa aaatcttgtc tgcaaaagtc
     8881 tcgattaact gagcttttac aaaagccgta tttcctggaa taacttggag attgatcatc
     8941 ttatccatca attcagccga ttcgatattg tcttcagcca gttgcagact ttttacgatt
     9001 gattttggta attcgtagac ataagtgttg tctctcaaag gaattttgac aatacctaac
     9061 tctttgatat ctcgggatac cgtcgcctga gtggcagtga tacctgcttc tttcaaatgt
     9121 tctacaattt cttcttgcgt gccgatttga taatctgtca ccaatcttct aattttttca
     9181 agtctttctt ttttattcat ttttaaattg actatgcgcc ctctctactg cttctttaat
     9241 ctcagcaaga atctgattgc ttgctgactt ttcttttttc aaatacgcta aaaattcaat
     9301 atttccatgt ccaccttgga tgggagaaaa gtccaagcca aggactgaaa aacctacctc
     9361 tactgccata gctgttacag attcaaggac attctgatga accttagcat ctcgaataat
     9421 tccatttttc ccaatctgct cacgtcctgc ctcaaactga ggtttgacaa gtgctaccac
     9481 ctgaccttga tcagccaaga cacggtgcaa ggctggcaaa atcagattaa gggaaatgaa
     9541 actcacatca atactggcaa agctcggctc ctgctcgaaa tcagtctttt cagcatagcg
     9601 gaaattgaac tgctccatgc tgacaactcg tgggtcttgg cgtaatttcc aagccaactg
     9661 attggtacca acatcgactg caaagaccaa cttggcacta ttctgtagca tgacatcggt
     9721 aaaacctcca gtagaggccc cgatatcaat cgtagtcgcg ccatccaccg acaaatcaaa
     9781 gacctgcaag gccttttcca gtttcaaacc accacggctg acatacttga gtttctcccc
     9841 cttgagtttt aattcggtgt catctggaat tttctctcct ggcttgtcaa accgttctcc
     9901 attaaggact gctacgacta ggccagccat cacacctcgc ttggcctgct ctctcgtttc
     9961 aaacaagccc tgtttataag ctagtacatc cactctttcc ttagccattg attctcaaac
    10021 tttctactac acttacaatc gattctgttt caaagggaag ctgctgggca atttcttcta
    10081 atttttcatt agcttgatcc agggtttggt tacaaaaggc aatggactct tccaagccca
    10141 acaaggcagg ataggttgat ttttctgcct gcagatcctt ttgaggtgtc ttgccgattt
    10201 cctcaaaact agctgtcaca tccagtacat catctctgac ttgaaaagca agtccaatca
    10261 attcacccac agttttcagc ttcacctgca tttcaggtga caattcagct ataatagctg
    10321 ccgcttggaa gggataggct agtaacttcc cagtcttatt ggcatgaata gtctgaagtt
    10381 cttccagaga caagtgctgg tgttcgccct ccatatccaa aacttgccct gccaccatac
    10441 ccagactacc tgaagcaagg gataagttgg caatcaagtc caccttgatc tgacttggca
    10501 aatctgcctg cgcaatcaag gcatatgggt ctaagaataa ggcatctcca gccaaaatgg
    10561 ccatagcttc accgaatttc ttgtgattgg ttaaccgccc tcttcgataa tcgtcatcat
    10621 ccatagcagg aaggtcatcg tgaatcaagc tccctgtatg aatcatctcc aaggcagtag
    10681 ctacctgcgc gtgagcaggt ttgatggcaa cctgcaagga ttccagaact tctaacaaga
    10741 gaaaaggccg aatacgcttg ccaccagcat gaatagaata gagaacagac tcccgtaaac
    10801 tagaggcaaa ctgctggtct ccataaaaat cttccaaagc cgactcgaca agagctaatt
    10861 tttcttgctt tttcattcaa aatcactttc tgttccgtct tcttgcatga ccttgaccaa
    10921 ggtcttttca gccttgtcca gcgtagcttg gagctctttt gacaagacca tgcccttttg
    10981 aaaggcagta atcgcatctt ccagagcaat ttcaccattt tccaaacttt ggacaatggt
    11041 ttccagttct gctagatttt cctcaaattt cttttgtttt gacatcttta acctctaatt
    11101 ctacttgacc atctcgcatc aaaagcgtta cttggtcttt tttcttcaaa ctctcaaccg
    11161 aatctacaat ggactcttct tttttgacaa tagcataacc acgcgccacg attcggctag
    11221 tatccaacat gagcaaagct tccgaaagtc gcttggcctc agcaaccttg gcgtcataaa
    11281 ctaacgccat ttggctacct aagagcttgt ccaactgtcc taaacggtct tgatagcgtt
    11341 ggattttggt aacaggtgat aattgtacta attgatgagt tcttgcttga actaattgtt
    11401 tgttatcaga aatccgagtt cgcaaacttt gtttcaaacg cagttgcagt tggtccaagc
    11461 gttgcaaata accgtcatac aagcgctcag gttgtctaaa gataacagac tgactgcatt
    11521 ttttcaaagc ctcttgtttc ttagatagaa catttcggac tgccgttgcc atccgttttt
    11581 cctgattttg caaatgagct aatacatcca acttggtcac aggtgttgcc agttcagccg
    11641 ccgctgttgg cgttgcagcg cgtcgatctg ccacaaaatc tgccaaggtc acatccgtct
    11701 catgccccac actagagata actggcaaac gagattcaaa aatagctcgt accacaattt
    11761 cttcgttaaa ggcccagaga tcctcaatag aaccacctcc acgaccaata atgagcaaat
    11821 ccaaatcatc ccgttgatta gcacgcgcaa tatttctagc aatttcctcc gcagcccctt
    11881 caccttgaac cttggtcgga taaagaagga tgtcaacacc tgggaatcgc ctgctgacgg
    11941 tcgtgataat atctcgaata acggctccac tacggctggt tactacccca attctcttag
    12001 aaaattgggg cagagcttgc ttgaagcgtt cttgaaacag gccttcttct gtcaattttt
    12061 tcttaagttg ttcaaactga atcgcaagcg ccccaacccc atcaggctca gctttttcaa
    12121 tgatgatgga gtagctacca cttggttcat aaacctgtac acgcccaatc acattgatct
    12181 tcattccttc ttccaggtca aaccctaatt tctgataaat cccagaccag atggtcgctt
    12241 gaataactgc atggtcatcc tttagggaga aatattggtg agtaggtcgt ttacgaaagt
    12301 tggaaacttg accagttaaa tagacccgtt ccaagtatgg gtctttatcg aatttcattt
    12361 tcagatactt ggtcaaagtt gttaccgata aatacttttc catctccacc tactattcat
    12421 ttacttgctc tttcatgggt attattatac caaaaatatg cctaaaaatc tccatttatg
    12481 taccattatg agggaaaaat agaaaaagga ggcaaggcct ccacatgtga ttatttgctg
    12541 tttcgagctt cttccaaaat ctttgcaatc ttggtcgtca acaggtcgat agccacggta
    12601 ttgctaaccc cttcaggaat gacgatatca gcataacgct tagttgactc gataaactgg
    12661 tggtacattg gtttgaccac acctaagtac tggttaataa cgctatcaag gctacggcca
    12721 cgctcctcca tatcacgctt gatacgacga ataatgcgca catcgtcatc cgtatccaca
    12781 aaaatcttga tatccatcaa atcgcgcaga cgcttgtcct ccaagaccaa aatgccctca
    12841 acgataaaga catcttgagg ctcctgacga taggtcttgc tactccgtgt atgctctgta
    12901 tagtcgtagg tcgggatgtc caccggacgc cctgccaaca attccttaat ctgctcgatc
    12961 atcaagtctg tatcaaaggc aaaaggatgg tcatagttgg ttttgacacg ctcttcaaag
    13021 gtcaaatgag actgatcctt gtagtatgaa tcatgctcaa tcatggaaat cttttcatca
    13081 ggaaaatgcg ataaaatggc tcttgaaaca ctggttttac caccaccaga accacctgtc
    13141 actccaataa tgattggtct attttgcatg ctatcctccg ttattttatc cgtcgtctat
    13201 tctatcacat tttttacaaa atttatagtc tcatcagcaa aaaacaccag agtattccct
    13261 ctgatgttcg acagttttaa cgaaagccct gtagggtttg agcaagcatc tgcaagaaag
    13321 ccagtctccc tctttcctgg ctaggagctt tggcaatcca taaaccaaaa tcaatgacag
    13381 acttaggtgc ctggtaatga tgcgtcatga ggtaaagtga caacccttga ctcaaattgg
    13441 ccatgaccga ttggtatttt tcatttctgt ctaaattttg tatagcaaga cgaaacaggt
    13501 gactcaactc actattttca tttatttctt tttgttgaag aagttgctcc accctttctc
    13561 gaaactcatt tcgcatcatg gaattatcac accaaatcct gggcctaagt tcccaccaga
    13621 acctgtaatg gactctaaat atttatctga aagaatttta aattgcttca tttcacacct
    13681 ctaaatttct atatagatta tttgagtacc tacaactcgc aacaattgga atattataat
    13741 tcccaaaacc attctactat tagtatgggg ttttatcaag caattgttat ctatcacccg
    13801 atttttattc ctaaacgatc tagctaaaaa ccttccttgg cttatagaga ttgccccgtt
    13861 tttctagaat ggctaacaat ttatcatctt caaaggcagc cagttctttg tccgtttggt
    13921 ctagctcaat aaaacgacca aagcgaactt ctgtagcctc ttctggagtt aggaaaactt
    13981 tgacaaggtc acctgtccca atctctaaag gatggagaaa atctaattgc ccagcctcta
    14041 ctttttcagc aatttcctcc aaggcaagag cgtcttctaa ttgtaagcca gcagcactag
    14101 tacgagtcaa atgggacata tgagccgcat aaccaagctt ttcacccaaa tcaactgaca
    14161 aagtacggat gtaagtccct ttactgcatt ttacacgaaa agtgaatcga gcaagttggc
    14221 catcataaga aatcggactt gttcgctcaa attgataaat ggtcacctga cgttctggac
    14281 gctccacttc ctgaccagca cgcgcatact catagagctt gcgaccatta accttaactg
    14341 ccgaatacat agggggaatc tgggtaatag gcccagtcaa gctagcaatc gcttcatcaa
    14401 caagcttttc atccaaggga gacaaaacag gggtttctgc gatcacttcc ccactagcat
    14461 cctcagtcgt cgtggaataa cccagagtaa tttccccctc atagatctta ccctcgtcct
    14521 gcataaactc gaccatgcgt gtcgccttgc caaccgcaat cggcaaaaca cccaccacat
    14581 ccggatccaa ggttccacca tgaccaattt tcttggttcc caaaatctta cgcagtttaa
    14641 aaaccgcatc atgcgaggtc attcctgctt ctttttttaa gttgataata ccgttcattt
    14701 ttgctttcta ctcccccttc agtgcttcga acttcgcttt tttccgggcc aatttttact
    14761 gaggcgtcgt ctacctttta ttctcttttt tacaaaattt aattaactat aattctttgt
    14821 aattcctcaa tctctggagg aggacaattg ttctgttttt gaatatgttg taatacattt
    14881 cgtctgtcat ctaaagataa taatgggagt atacttttga tgccatctaa ataaaaaata
    14941 tcattctcta ttttatcggt ctccattaat ttcagaaaca tttttttcaa caaatcaaaa
    15001 ttgttaagtt cttcatgatt caatttataa ttcatccctc gtaaagataa tttctggtta
    15061 taagttgtta agtaatcact caattcctct ataaacaaag caaatctatt ttttttaaga
    15121 ttttcattat ataataaata aaaaattgtc accaaataat ttgcacttga gtcaaatttg
    15181 aataatgctc ccaaccattc tttagaatat ctaaattcat agtctcctgt aaaaatgatg
    15241 tttatcattt gactatagtt attctttgat aaagaaaatg attcaatagt tggatctacc
    15301 ctctctaaag caactagata aactttatta agaatttgat tgtcctcaat ttttttgttt
    15361 agaatattcc ttactacatc tgtgaaaaca tccgcagctt cgatagtaat tgtagaatga
    15421 taatacgcaa atcctaaaaa ggtgttgtaa gttacattga ttctgtttgg attgtattca
    15481 tttaataaaa taccttgagc aaactcccta taatcggtat ctgttactga atttattatt
    15541 ggaataatta catctctata acttgaatga tttataaata atgatattaa acattgaaaa
    15601 tatgatccca tttcactatt tataaaatga agtatatcta attgtttatt atcacctttt
    15661 ggtgttgaga gttcattcac attgatacta agtaattttt caaaaatatc tatatccgta
    15721 ttttgttgtt ttatatgata ttcaaagaat ttccgatcgt tagaatcaaa actttctttg
    15781 ttaaatcttt ttaaatattt ttctctaaca atctttctca aattttcttc tatttcagga
    15841 aaactgataa tttttacata taatctctta tattttggat ataagtcatc tatatgattt
    15901 ttcaataaat cagaaacttt tttagatatt tcatttgatt cttttaaaac agtaataaag
    15961 aaattttccg tttcatttat cgtcttttct tccaaaaatg aagaacctct ttgttccttt
    16021 tcggcgttga ctaatatctc taatactcta gattcatcgt actctacaag attttcttga
    16081 gttaaatatg aaaattgtga aacaaaacct ccttctagtg aagatatcca atttctataa
    16141 ggtttttttg gtatttcaat atttttttct ctaataatac ttctatgttc tttgataaaa
    16201 gtgtttaatt cctctccaaa gtgaatattt gaaaaatcaa ttttatcaat taacctcttg
    16261 ataaattcta aatccaaatt aatttcatta tccaattgaa caagcaattt ttgaattaat
    16321 atagtactta ttaagtcttc agaatcactt tctaaaaact tttcctcttc aataaataat
    16381 tttttggact ttacaagctc ataaagaaat ttaacaacat catactcttt taattgatgc
    16441 tctatagagt agtaattata acttaaaaat tctggattat ttagtatgga cattaatttg
    16501 ttaacacatt cgtaatttaa gttaacttct acttcactat cttcctctat atataaatca
    16561 cgattttgct gttcaaaagc acttactagc cagtaaccca aaagagtttt atctttatta
    16621 aagttagaat taacgatata tccatctttg gatataattt ctctcagttc attaagctta
    16681 ttattatttg taaaactaga aattttatta tctatcgcat aattatagac aaagaataca
    16741 tcatctataa agtaattttg agagccttca taaattattt tataaatcac atcccactca
    16801 ttatcactta acttttcaat attcttaaca ataaacttcc aaaatgcaag aacaaaattt
    16861 ttttaattac tgttagagtt tcactccgaa gcagagcatt taacattagc tgatctagtt
    16921 gattattatc attttcaata accttatcta aaacagagct tagatactta aaatcattag
    16981 aaatactatc tagattttta ttatactctt cttgatttat agaaggattc aaaagattgt
    17041 tccactgatt ataaaaatca tgtgtgccca atttttcatt aatatcagca actaattttt
    17101 ttacaaaaag aggtagtttc tcaaattcat ctccgtacca aataatctga gtatgatttt
    17161 ctgagctaaa aaatccttca acaacacctc tataatgttc atcaactctc tgattacctg
    17221 tgttctctga cttcatcaag gcatagttct tactatcttt acaaagtaaa gataaaattt
    17281 catcttcttc aagacctgct ccaataaata aaacagtagg ttttgtctct ttaaaccatg
    17341 ttactaaatt ttcaaaattt tccctatttt ttaaataggt ttttgaataa tctgcggatg
    17401 aacttacaaa atactttaca tcacaatctg gtgttccatg gatatgtaga acatcaccaa
    17461 attgtagttt cccattcttg ttcttagtga attcattgag atcattaata gtaactacag
    17521 cattctttaa acgtttaata tgattttcaa tttcatagtc gtagttagaa gtaataaata
    17581 ttgcattcaa acttgctaaa gattctactg tttgattaac tattgaatat ggtaatagat
    17641 ttttaaaata tcctttttca aaatctagtc gtcgcttttc aaaatcttca ccaaaaactt
    17701 tttttaattc atagtttaca agatccactt tacgtttatt actaatacta gatttgctaa
    17761 ttaaatcaaa aactacgtga tgttctcttc caagcctttt ttcaccactt accgataaaa
    17821 cttgtccttg ccaatactta ataagatgtt caatataatt gttccaatta ggataacctt
    17881 gagcaattga aactcctgcg ccaataaaaa ttactaagtt aaactttttt atagcctcgg
    17941 ttacatcatc catcaaattt tcataattaa gatcatcttc cataacttat ttcctcactc
    18001 ccccttcagt gcttcgaact tcgctttcat tgttggattt ttccgggtca attttttaac
    18061 agagacatct tccaatttgt tgttagctag acggagttgg ttttcagatg tagtcaggaa
    18121 tttcttaacc tcttccatgc gtttgatggc cttgtcgatt tcatcaatag cttttccaaa
    18181 gttagtcgaa gctgaattat agttcttagc aaaagctagc ttaaaggcat ccaagtcttc
    18241 ctcaaaatgc gtaatgtcaa tattttgctc gcgaaccaag gccaactcct gcttgtattt
    18301 tagggaattt agcgccgcat tacgtaagag accaatcaat tggataaaga attgaggacg
    18361 aacaacatac attttttcat actcgtgact gacgtcaaca atccctgtgt taaagtagtc
    18421 attatcagcc tcaagcatgg tcaccaaaac ggcatactca cagttcttct cccgacggtc
    18481 cttgtccaat tccttgtaaa aatctgcatt cttgtgcttc ttctctgttc cgtccgcttc
    18541 gtttttcatc tcaaacatga tagaaatgat ttcaactcca ttttcatcac actcacggaa
    18601 gataaagtcc cctttagacc cacgcgaaga gaccttgtta tccttctcaa agtaagcatt
    18661 tggaaaggcg aaactacgaa ccttgttaaa ctcactctct gcatactgtt ctaggctttc
    18721 cccaatcgct tttgtagatt gttgagcctt aaaattctta taaaactcga cttgttcact
    18781 ggctgctttg agctgggctt cgtagttttg cttaacagaa gccaaagaca actcattttc
    18841 cttttcttgc agtaggagct gatttttgac ctgattgcgt tctttttcta ggtcagaaag
    18901 ggtcttttgc aattgatttt catgctccaa acgcaaggtc gccaactggt tttccaaggc
    18961 ctgtacttcc ttgtccttag ccaataaggc ctgatggctt gtctgttcaa cttctttctt
    19021 ggccaattct ttttctgtat caaagttttg gatttgactc tgcaattgcg caatttcttg
    19081 atccttctga gccagtttag tctgttgctc attcatagcc ttttgctcag ccaaggccag
    19141 ttcctgcctc atcctatcgt gtagttcctt atcaaactct gtcgttctca cttgggacaa
    19201 aagttcagca tactggcttt catttactgt aaagacttcc ccacagttgg gacatttgat
    19261 ttcgttcatt acattcctct ttctagactt ctgtaaccat tatatcatgc ctgagggaaa
    19321 atcaaaaaca gtgagtcaaa acccactgtt tatcttcttt tactttaaat tttttccaag
    19381 gccaagcgta agtcttcaat caaatcttct acattttcaa gaccgataga caagtggatt
    19441 tgatttggtg tgacacctgc tgcttctagg tctttttctg acaattgacc gtgagtgatt
    19501 gttgctggat ggacaacaag cgatttagca tctgctgcgt ttgcaaggtc agaaaagatt
    19561 tctaaattat caatgacctt gcgtgcttcc tcctcgtcgc ctttgacgtg gaaggtaaag
    19621 attgaaccga caccttttgg caagtatttc tcagccaagg cataataagg actatctgca
    19681 agttttggat aatttacctt ttctacctta ggatggttga caagaaaatc aacaattgtc
    19741 tcagcatttt gtacatggcg ttccacacga agtgaaaggg tttcaagtct ttgtagcaag
    19801 aggaaagcat tgaatggtga caaggatgca cctgtatcac gaagcaattg aactcgaaca
    19861 gctataataa aggctgctgc acccacatca tgagtatagc tcaaattgtg gcagcttgga
    19921 ctctcgtcaa caaattgagg gaatttccct gaagccgtcc agtcaaaacg accactatcg
    19981 acaattattc ctccaatagt tgtaccatgc ccaccgataa acttagtcgc agagtgaatg
    20041 gcaatgtcaa cgccatgaga gaagacgtta atcaaataag gtgttgcaaa agtattgtct
    20101 gacacaagtg ggatttgatg tttatgagca atctctgcca gtttttccag gtctggaata
    20161 ttaatcaagg ggttacccaa ggtttcaatc aagacaagct tggtattgtc tttgatagct
    20221 gcttctactt cctccaaatt atcaacatcg acaaaggttg ttgtgatacc ataacgagga
    20281 aggggttttt tcaaaagatt gaaggttcca ccgtaaatag tcgaagcagc cactacatgc
    20341 tcaccagcat gggcaagcgc caaaatcgta taagtcactg cagtcatacc tgatgctgtt
    20401 gctagcgctt caacaccacc ttcaagggca gctgttgtag gattggtgat acgagtataa
    20461 atgttccctg gtttcctcaa ggcaaacaga tcggcacctt cctacgtgtc atcaaaaaca
    20521 aaaaatgttg tttgataaat cggcactgca cgagacttag tagctggagc cacaacttga
    20581 ccagcatgta gttgtagagt ttcaaattta aaatcacaag tcataaggtg acctccatat
    20641 cattttatta aggatattgt agcaccttga gttatagttt acaaatatca gttttctata
    20701 ttttgatata agaaatagct attaccttat tttgaaaatg aaaaaagcac gactcaatcg
    20761 tgctcatttt cttaatctac ataagtatct tcgtttttat tgaggaaggc atatggaaga
    20821 cccatcaaga ggcctcctcc gataaagttt ccgatgaaag tcacacccca gtggcg
//
