LOCUS       NZ_CP113114            10300 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Streptococcus pneumoniae strain NP1 chromosome, complete genome.
ACCESSION   NZ_CP113114
VERSION     NZ_CP113114.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Genome sequencing and assembly of Streptococcus pneumoniae strain
            NP1 isolated from nasopharynx
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2022) Community Medicine &SPH, Post Graduate
            Institute of Medical Education & Research, Chandigarh, Room No 529,
            Research Block B, PGIMER, Sector 12, Chandigarh, Chandigarh 160012,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: MaSuRCA v. 3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 356.0x
            Sequencing Technology :: Illumina Novoseq 600; Nanopore GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 02:39:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,409
            CDSs (total)                      :: 2,335
            Genes (coding)                    :: 2,154
            CDSs (with protein)               :: 2,154
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 86 of 181
            Pseudo Genes (incomplete)         :: 87 of 181
            Pseudo Genes (internal stop)      :: 72 of 181
            Pseudo Genes (multiple problems)  :: 53 of 181
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:47:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 751741
            Orig. end                         :: 762041
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10300
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10300
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10300
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5300](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5300
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            631..900
                     /locus_tag="OTU45_RS03725"
                     /old_locus_tag="OTU45_03725"
     CDS             631..900
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /locus_tag="OTU45_RS03725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03725"
                     /product="YqgQ family protein"
                     /protein_id="WP_000051787.1"
                     /transl_table=11
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIEL
                     MKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            900..1280
                     /locus_tag="OTU45_RS03730"
                     /old_locus_tag="OTU45_03730"
     CDS             900..1280
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /locus_tag="OTU45_RS03730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03730"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_000259256.1"
                     /transl_table=11
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQL
                     IDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLKKQ
                     GFSEIYILSYGLDSWKGKVKTS"
     gene            1369..1539
                     /locus_tag="OTU45_RS03735"
                     /old_locus_tag="OTU45_03735"
     CDS             1369..1539
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /locus_tag="OTU45_RS03735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03735"
                     /product="hypothetical protein"
                     /protein_id="WP_050089826.1"
                     /transl_table=11
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKESQ
                     LDYKIEKILNI"
     gene            complement(1636..2361)
                     /locus_tag="OTU45_RS03740"
                     /old_locus_tag="OTU45_03740"
     CDS             complement(1636..2361)
                     /EC_number="5.4.99.-"
                     /GO_function="GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1154: Pseudouridine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /locus_tag="OTU45_RS03740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03740"
                     /product="pseudouridine synthase"
                     /protein_id="WP_001235044.1"
                     /transl_table=11
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGLQ
                     ELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLDRD
                     TTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVCKPA
                     KLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAEGNY
                     RPLNQKELQIIKNYLEMSR"
     gene            2523..4364
                     /gene="typA"
                     /locus_tag="OTU45_RS03745"
                     /old_locus_tag="OTU45_03745"
     CDS             2523..4364
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /GO_process="GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="typA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /locus_tag="OTU45_RS03745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03745"
                     /product="translational GTPase TypA"
                     /protein_id="WP_000164111.1"
                     /transl_table=11
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAERA
                     MDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVVDA
                     YEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQLDF
                     PVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLDYNDF
                     VGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAGDLIAV
                     SGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSRKVEERL
                     QAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEVIVKEIDG
                     VKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPARGLIGYST
                     EFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMSIEERGTIFV
                     NPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPRILTLEESLEF
                     LNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            4384..4638
                     /locus_tag="OTU45_RS03750"
                     /old_locus_tag="OTU45_03750"
     CDS             4384..4638
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /locus_tag="OTU45_RS03750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03750"
                     /product="DUF3165 family protein"
                     /protein_id="WP_000262800.1"
                     /transl_table=11
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVLS
                     FLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     misc_feature    4801..4814
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.31"
                     /tool="antismash"
     misc_feature    complement(4804..4820)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.88"
                     /tool="antismash"
     gene            5001..5300
                     /locus_tag="OTU45_RS03755"
                     /old_locus_tag="OTU45_03755"
     CDS             5001..5300
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /locus_tag="OTU45_RS03755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03755"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000850390.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2e-25, bitscore:
                     86.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGGW
                     AFSNYYHGKKYHYSSVVSKWDSHSDKGSAPAGKTSEAWIWTKFGEQVSFYYDYD"
     gene            5371..7392
                     /locus_tag="OTU45_RS03760"
                     /old_locus_tag="OTU45_03760"
     CDS             5371..7392
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /locus_tag="OTU45_RS03760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03760"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000732401.1"
                     /transl_table=11
                     /translation="MKKLFILLSTFFLSFFFAWIIVLRAPQYLYASYDSVTLLRVKKEA
                     QEPTREVFEQELEKFVNSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKQFQEASQES
                     RERSDPLNSYLLLSGSLTKEKLADKLGDLGYKAIADRKTPPYSLAFRMLLNPLILISLA
                     IFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFLGGGV
                     VLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGRLPLKR
                     LMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWHDRVDYYQISFGLGDRVND
                     TENQNKWYEFSKEAVEKEQALFVKENLIHFANPQGKNENGETLDTYSPDANVLYVSPSY
                     LDKENVAVNDETRQKLAHLQKGEFGLLLPESLRSQEAELKKVFEERLNYYGKSSEDKDA
                     PLEYEMRAIVSYLPTGQKRFVYNNGESPVSIQYLTDPILVVFTPTSTGDSFISKYVWSI
                     NAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETATLILGAIVGI
                     ASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFVFGASFFILSSR
                     DLVIGLLTLFVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            7394..8035
                     /locus_tag="OTU45_RS03765"
                     /old_locus_tag="OTU45_03765"
     CDS             7394..8035
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /locus_tag="OTU45_RS03765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03765"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571289.1"
                     /transl_table=11
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLLN
                     MIGRLEPYDKGQIIYDGTSLKDIKSSVFFRDYLGYLFQDFGLIESQTVKENLNLGLVGK
                     KLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILADEPT
                     ASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            8139..9491
                     /gene="murD"
                     /locus_tag="OTU45_RS03770"
                     /old_locus_tag="OTU45_03770"
     CDS             8139..9491
                     /EC_number="6.3.2.9"
                     /GO_function="GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="murD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /locus_tag="OTU45_RS03770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03770"
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="WP_267750352.1"
                     /transl_table=11
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFED
                     NPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTEVE
                     LAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATDKNT
                     LVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVL
                     NFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVE
                     NALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKA
                     LSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEA
                     TDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE"
     misc_feature    complement(9883..9897)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.66"
                     /tool="antismash"
ORIGIN
        1 caccttgtct ttctttgcca gagggttggt cttgctgaca aagatatgcg gttgcgctgt
       61 gaagagatgg tgggctagca ggtgattgtc atccagcatc ttggttaaaa catcacggtt
      121 gtagctgttc aaaaatagga caccgacctc actacggaaa ttcttgacgt cgtcgataat
      181 ttcccaagta cgagtttcac gaaggaagag ttcgtatttc tccatatcgc ttttcttgag
      241 caaagagaca aaggcattga ccacaaaggc atagtgttga gacgaaacgc taaagagttc
      301 gcggtgggcg acaggatttt tatagcgttc ctccagaagc tgagtttgct cgacaacctg
      361 acgggcataa gagagaaact ccatgccatc acgggtcaag gtgattccct tgggattgcg
      421 gataaagatc tcaatgccca tttcattttc caaatctctc acagcattgg aaagacttgg
      481 ctgagtgata aagagttgct tggctgcctc attcatggag ccagtttcga cgattttgat
      541 aatataatgt aattgttgaa ttctcatgtt tttattgtac catacttgcc ttagaatcgc
      601 taaagaagat aggaaaataa ggagcattgt gtgagtctct tcttgaaaaa aacacaaaat
      661 tttgctagaa tgagtcttat gaaaacattc tatgatgtgc agcaattcct caaacgattt
      721 ggtattattg tttacatggg aaaacgctta tatgatattg aactgatgaa gttggaacta
      781 tctcggattt acgatgcagg gttgatggat aaattagact atctagaggc agaagcagtt
      841 cttcgcagag agcacaaggt agaattggat tatattgaga aaaatggaga aaagaactaa
      901 tggttacttg gattttgtgg gcacttatac tagcaatgtt ggcgtggatg ggctttaact
      961 atcttcgtat tcgccgtgcg gctaaaattg tggacaatga ggagtttgaa gccttgattc
     1021 gtacgggtca attgattgat ttgcgcgacc cagcagaatt ccacagaaaa catatccttg
     1081 gtgcacgcaa tattccttca agtcagttga aaactagtct tgcagccctt cgtaaagata
     1141 aacctgtcct tctctacgaa aaccaacgtg cgcaacgagt tacaaatgca gctctttact
     1201 tgaaaaaaca aggtttttct gagatttata tcctttctta tggcttggat tcttggaaag
     1261 ggaaagtgaa gactagctaa ccttatttcc aaattagtcg aatgatagga tgaagctatt
     1321 gttttatagt ataattaatt tgtataaaat ttaaggagtt cttagaacat gaaagaaaag
     1381 aagaaaaatc cactttttgt gggtatcttg tcgattattc ttggtttgtt atttccaatt
     1441 gtaggcctga ttttgggtat cataggattg gtcttggcta tttcatacca aaaagagtca
     1501 caattagact ataaaataga aaagattctt aatatctaag gaattgtgat ttctgtagtt
     1561 aactggattg tagcaatcgc cttaattttt cggtaaataa aagaaaaaag ctttagtttt
     1621 aaagcttttt ttgttttatc gactcatctc taaatagttt ttaatgattt gtaactcttt
     1681 ttggttcaaa gggcggtagt taccttctgc taaatctggg ttcaatgtga agtccccaaa
     1741 ttggattctt ttgaggctag tcaccttaac accaaccgag aggaacattt tcttgatttg
     1801 atgaaatttt ccttctgaaa tggtgataga ggcttgactg agggagggac ttgcagatag
     1861 aatctctagt tttgcgggtt tacagacagt accatctaaa aagacaattc ccttttgaaa
     1921 ggcttggata tggtcaggtg ttagaagtcc attaacctgg acttggtaag tcttatcgac
     1981 atgatattgg ggatggagga gctgaaagcc caagggaccg ttatcggtca agaggaggag
     2041 tcccgttgta tctcggtcca gtcggccaac ggcatagagc ttgtcagact ggatgtcaga
     2101 tggaagcagg tccatgacgg tcggaagttc cttgtctttg ttggctgtaa cgacaccagc
     2161 aggtttatga agcataagat aggtgtgttc atagccttga atgattcgat cctgaaaaag
     2221 gagttcttgt agtcctgtat cgatattttg agctagggag cgggctgggc aaccatcgac
     2281 tagaatttcc cctctgagga gtgcttgctt catggccttt cggctaactt tttcttgggc
     2341 taataaatta tctaaacgca tactgtgcct atcttatcat aagtcgcttg ctttgaaaag
     2401 gcttgacgtg aaaagaaaag cggagtgaaa acatttacac ttgcttgagt tatgttattt
     2461 atttgaaatt atggtataat cgttcagtta gaaaataaat tttgaatatt atagaggaaa
     2521 tcatgacaaa attaagagaa gatatccgta acattgcgat tatcgcccac gttgaccacg
     2581 gtaaaacaac cctggttgac gaattattga aacaatcaga aacgcttgat gcacgtactg
     2641 aattggcaga gcgtgctatg gactcaaacg atatcgaaaa agagcgtgga atcaccatcc
     2701 ttgctaaaaa tactgccgtt gcttacaacg gaactcgtat caacattatg gacacaccag
     2761 gacacgcgga cttcggtgga gaagttgagc gtatcatgaa aatggttgac ggtgttgtct
     2821 tggtcgtaga tgcctatgaa ggaaccatgc cacaaactcg tttcgtattg aaaaaagcct
     2881 tggaacaaga ccttgtccca atcgtggttg ttaacaaaat cgataagcca tcagctcgtc
     2941 cagcagaagt agtggatgaa gtcttggaac ttttcatcga gcttggtgca gatgacgacc
     3001 agcttgattt cccagtggtt tatgcttcag cgatcaacgg aacttcttca ttgtcagatg
     3061 atccagctga ccaagaagcg actatggcac caatctttga cacgattatc gaccatatcc
     3121 cagctccagt agataactca gatgagcctt tgcagttcca agtgtcgctt ttggactaca
     3181 atgacttcgt tggacgtatc ggtatcggtc gtgtcttccg tggtacagtt aaggttgggg
     3241 accaagttac cctttctaaa cttgacggta caactaaaaa cttccgtgtt acaaaactct
     3301 tcggtttctt tggtttggaa cgtcgtgaaa tccaagaagc caaagcgggt gacttgattg
     3361 ccgtttcagg tatggaagac atctttgtcg gtgaaaccat cactccgaca gatgcagtag
     3421 aagctcttcc aatcctacac atcgatgagc caactcttca aatgactttc ttggtcaaca
     3481 actcaccatt tgctggtaaa gaaggtaaat gggtaacttc tcgtaaggtg gaagaacgct
     3541 tgcaggcaga attgcaaaca gacgtttccc ttcgtgttga cccaactgat tcaccagata
     3601 aatggactgt ttcaggacgt ggagaattgc acttgtcaat ccttatcgaa acaatgcgtc
     3661 gtgagggcta tgaacttcaa gtatctcgtc cagaagttat cgtaaaagaa atcgacggtg
     3721 ttaaatgtga accatttgaa cgtgtacaaa tcgacactcc agaagaatac caagggtctg
     3781 ttatccaaag cctttctgaa cgtaaaggtg aaatgttgga tatgatttca actggtaatg
     3841 gtcaaactcg tttggtcttc cttgttccag cgcgtggttt gattggatac tcaactgagt
     3901 tcttgtcaat gactcgtggt tacggtatca tgaaccatac cttcgaccaa tacttgccat
     3961 tgattccagg ggaaattggt ggacgtcacc gtggtgccct tgtttctatc gatgctggta
     4021 aggctacaac ttactcaatc atgtctatcg aagaacgtgg tacgatcttt gtcaacccag
     4081 gtactgaggt ttatgaagga atgatcatcg gtgaaaactc tcgtgaaaat gacttgacag
     4141 ttaacatcac gaaggcaaaa caaatgacca acgttcgttc agctactaag gaccaaacag
     4201 ctgttatcaa gacgcctcgt atcttgacac ttgaagagtc tcttgagttc ttgaacgacg
     4261 atgagtacat ggaagtaacg cctgagtcta tccgtttgcg taaacaaatc cttaacaaag
     4321 cagagcgtga gaaagctaac aagaagaaaa aatcagctga ataagagcta gaaagagaga
     4381 aagatggtct atttagtcct aggaatttta ctgctcctac tctatgtatt tgcgacacca
     4441 gaaagcatta aagggactgt caatatcgtc gctatggtat gtattttagt ggcactcttg
     4501 attttattgg ttctatcttt tctgaaaatt tttcaattac caacagaaat attcctagca
     4561 atagccatgt tgatcctagc ttactttagt gttagagaca tcacactcat gccagtcaaa
     4621 aaaagtaaaa gaagataaaa aagagagttt cggctctctt tttgctagga attttacaat
     4681 agtttgaatt gctttttagt taacaccatc tcttttactt gtatagtatg aatgggaaaa
     4741 acttgaataa aattcgttaa tggcatctat tttatatttg gttagtactg gttgcatatt
     4801 attttgaaat aaaactacta tataatatta ttcacagagg taatatagta aaatatctaa
     4861 aaaattcgat tttttccttg acaacaaata ataaaaatgc tattcttatt atataagaaa
     4921 tcggtttcaa aaaagagaga ggaggattta ttttgttgca ttgttgctca gggtgtgctt
     4981 tttttgaaag aaaaaataaa atgaaaacga aaaaacatag attacttgct ctagctctta
     5041 tttcaagctt tacattattg ggagctgcat cagctgctgt acaatatcca gatggaggag
     5101 tatggacata tggagaaggt tcaggaggtg gttgggcttt ttcaaattac tatcatggta
     5161 aaaaatatca ttattcttct gttgtaagta aatgggatag tcattcagat aaaggatcag
     5221 cacctgcagg gaaaacttca gaggcttgga tttggactaa atttggggaa caagtatcat
     5281 tttactatga ctatgactaa tatttagttt tcttagataa ttccccttgt aagaaattac
     5341 agggggaatt ttgtttacaa ggagtctact atgaagaaac tattcatatt actatcaact
     5401 ttttttctca gcttcttctt tgcttggatt attgtcttac gtgcgccaca atatttatat
     5461 gcaagctatg attccgttac tttacttcgt gtcaaaaaag aggctcagga accgactcgt
     5521 gaggtatttg aacaggaatt ggagaagttt gtaaactcag aacagagttt aatagctagg
     5581 agaattgtag aaccgagtaa ggatgggacg actcacttta cttatgcaac ttatggtcag
     5641 ggaacattac caaaacaatt ccaagaagct agccaagaaa gtcgtgaacg tagtgatccg
     5701 ctaaatagtt atctcctttt atcaggctcc ttgacaaaag aaaagctagc tgataaatta
     5761 ggagatttag gttataaagc aattgctgac cgaaagacac ctccctattc tcttgctttt
     5821 cgaatgttac taaatcccct tattttaatt agtttagcaa tatttggctt atctttcttt
     5881 gctttagtga ttatcactcg gattaaggaa atgagagcag caggtataaa actcttttct
     5941 ggtcagactc tcttatccat tatggggcat tctttatcta ctgatattaa gtggctcctt
     6001 ctatcagccc tcctttcctt cctaggtggg ggagtcgttc tttttagtca aggtttgttt
     6061 tatcctatct tgttagccac ctatggtttt gggattagtt tctatctgtt gtttttattg
     6121 gcgatttcaa ttttactaat gctcctttat ctaatgagtt tgagttacaa agcattagtt
     6181 cccgttatta agggaagatt accccttaaa cgcctgatga ttttaaccct attgtgtcag
     6241 ttggttgctg tctttacagt gggatacgct gttaagacag gtttgacgtc ttaccaacga
     6301 ttgaaagaac ttgaaatttc aaaacaagca tggcatgaca gagtagacta ttatcaaatt
     6361 tcttttggct taggtgacag agtaaatgat acagaaaatc agaataagtg gtatgaattt
     6421 tccaaagaag cagttgaaaa agaacaggct ctatttgtaa aagagaatct cattcatttt
     6481 gcaaatcctc aaggaaaaaa tgaaaacggg gaaacactgg atacctatag tccagatgct
     6541 aatgttctct atgtgagtcc aagttattta gacaaggaaa atgtggctgt aaatgatgag
     6601 actagacaga aactagctca tcttcaaaaa ggggaatttg gactcttatt accagaatct
     6661 cttcgttcac aggaagcaga acttaagaaa gtttttgaag aacgtttgaa ctattacgga
     6721 aaatcaagcg aggataaaga cgctccttta gagtatgaaa tgagagcgat tgttagctat
     6781 cttccaacag gacaaaaacg ctttgtttat aataacggtg aaagtccagt atctattcag
     6841 tacttaaccg atccgatttt agttgtattt actccaacat ctacaggtga tagtttcatt
     6901 tcaaaatatg tttggtctat caatgctgga aaacaactct ttatcaaagg atatgagagt
     6961 gggctagaac tcttaaagaa agctggaatt tatgagcaag tatcctatct taaagaagga
     7021 agaagtgttt atctaactcg ttataatgaa gttcaaactg aaacagcaac tttaatctta
     7081 ggagctattg ttgggatagc cagttcctta ttactctttt attctgtcaa tcttctatat
     7141 ttcgagcaat tccgccgaga tatcttgatt aaacgaattt caggtttacg attttttgaa
     7201 acacatgctc agtatatggt tagccaattt gccagttttg tatttggtgc tagtttcttt
     7261 attttaagca gtcgagactt ggtgattggc ttgctcactt tattcgtctt tctagctagt
     7321 gcagttttga cgctttaccg tcaagcacag aaagaatctc gtgtttctat gacaattatg
     7381 aaaggaaaat aggatgattg aactaaagaa tatatctaaa aaatttggaa gccgtcagct
     7441 attttcagat acaaatcttc attttgaagg tgggaaaatt tatgccttaa tcggtacaag
     7501 tggctgtggt aagacaacac tcttgaatat gattggacga ttagagccat atgacaaagg
     7561 gcaaatcatc tatgatggca cttctcttaa ggacatcaag tcttctgttt tctttagaga
     7621 ttacttggga tacttatttc aagattttgg cttaattgaa agccaaaccg tcaaagagaa
     7681 tctcaatctg ggtttagttg gtaaaaagtt gaaggaaaaa gagaaaatct ctttgatgaa
     7741 acaagctcta aaccgtgtaa acctctctta tttggatttg aagcaaccta tatttgagtt
     7801 atcaggagga gaagcacaac gtgttgcatt agcgaagata attttaaagg atccgccttt
     7861 gattcttgca gatgaaccaa ccgcttcctt agaccccaaa aactctgagg aattactttc
     7921 catcctagaa tctttaaaaa atccgaatcg gaccattatt attgcgaccc acaatcctct
     7981 gatttgggag caagtggatc aggtcattcg agttaccgat ttatcacata gatgatatgg
     8041 taagattcag ttagaagaaa gagtcacaaa cacactttgt ggctttttta tttccataaa
     8101 aatggtaaaa tagtaggagt agaaatggag ttcgagacat gaaagtaata gatcaattta
     8161 aaaataagaa agttcttgtt ttaggtttgg ccaagtctgg tgaatctgcg gctcgtttgt
     8221 tggacaagtt aggtgccatt gtgacagtaa atgatgggaa accattcgag gacaatccag
     8281 ctgcccaatg tttgctggaa gaagggatca aggtcattac aggtggccat cctttggaac
     8341 tcttggatga agagtttgcc ctcatggtaa aaaatccagg tatcccctac agcaatccca
     8401 tgattgaaaa ggctttggcc aagggaattc cagtcttgac tgaggtggaa ttggcttatt
     8461 tgatttcaga agcaccgatt attggtatca caggatcgaa cggtaagaca accacaacga
     8521 ctatgattgg ggaagttttg actgctgctg ggcaacatgg tcttttatca gggaatatcg
     8581 gctatcctgc cagtcaggtt gctcaaatag cgacagacaa gaacacgctt gttatggaac
     8641 tttcttcttt ccaactcatg ggtgttcaag aattccatcc agagattgcg gttattacca
     8701 acctcatgcc aactcatatc gactaccatg ggtcattttc tgaatatgta gcagccaagt
     8761 ggaatatcca gaacaagatg acagcagctg atttccttgt cttgaacttt aatcaagact
     8821 tggcaaaaga cttgacttcc aagacagaag ccactgttgt accattttca acacttgaaa
     8881 aggttgatgg agcttatctg gaagatggtc aactctactt ccgtggtgaa gtagtcatgg
     8941 cagcgaatga aatcggtgtt ccaggtagcc acaatgtgga aaatgccctt gcgactattg
     9001 ctgtagccaa gcttcgtgat gtggacaatc aaaccatcaa ggaaactctt tcagccttcg
     9061 gtggtgtcaa acaccgtctc cagtttgtgg atgacatcaa gggtgttaaa ttctataacg
     9121 acagtaaatc aactaatatc ttggctactc aaaaagcctt atcaggattt gacaacagca
     9181 aggtcgtctt gattgcaggt ggtttggacc gtggcaatga gtttgacgaa ttggtgccag
     9241 acattactgg actcaagaag atggtcatcc tgggtcaatc tgcagaacgt gtcaaacggg
     9301 cagcagacaa ggctggtgtc gcttatgtgg aggcgacaga tattgcagat gcgacccgca
     9361 aggcctatga gcttgcgact caaggagatg tggttcttct tagtcctgcc aatgccagct
     9421 gggatatgta tgctaacttt gaagtacgtg gcgacctctt tatcgacaca gtagcggagt
     9481 taaaagaata aaatatgaaa aaaattgtct ttacaggtgg ggggacggtt ggacacgtta
     9541 ccctcaatct tttgttaatg cccaagttca tcgaagatgg ttgggaagtc cactatatcg
     9601 gggacaagcg tggtatcgaa caccaagaaa tccttaagtc aggtttggat gtcactttcc
     9661 actccattgc gactgggaaa ttgcgtcgct atttctcttg gcaaaatatg ctggacgtct
     9721 tcaaagttgg ctggggaatc gtccaatcgc tctttatcat gttgcgactt cgtccacaga
     9781 cccttttttc aaaggggggc tttgtctcag taccgcctgt tatcgcagcg cgtgtgtcag
     9841 gagtgcctgt ctttattcac gaatctgacc tgtctatggg cttggccaat aaaatcgcct
     9901 ataaatttgc gactaagatg tattcaacct ttgagcaagc ttcgagtttg tctaaggttg
     9961 agcatgtggg agcagtgacc aaggtttcag atcaaaaaaa tccagaacca gatgaattgg
    10021 tggatattca aacccacttt aatcataaat tgccgactgt attgtttgtt ggcggttctg
    10081 caggtgctcg tgtctttaac caattggtga cagaccataa gaaagaacta acagagcgct
    10141 acaatattat caatctaact ggagattcta gcctgaatga gttgagccaa aatctttttc
    10201 gtgttgacta tgtgaccgat ctctatcaac ccttgatgga attggctgat attgttgtga
    10261 cacgaggtgg tgccaatacg atttttgagc tcttggcgat
//
