LOCUS       NZ_CP113114            17955 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Streptococcus pneumoniae strain NP1 chromosome, complete genome.
ACCESSION   NZ_CP113114
VERSION     NZ_CP113114.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Genome sequencing and assembly of Streptococcus pneumoniae strain
            NP1 isolated from nasopharynx
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2022) Community Medicine &SPH, Post Graduate
            Institute of Medical Education & Research, Chandigarh, Room No 529,
            Research Block B, PGIMER, Sector 12, Chandigarh, Chandigarh 160012,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: MaSuRCA v. 3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 356.0x
            Sequencing Technology :: Illumina Novoseq 600; Nanopore GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 02:39:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,409
            CDSs (total)                      :: 2,335
            Genes (coding)                    :: 2,154
            CDSs (with protein)               :: 2,154
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 86 of 181
            Pseudo Genes (incomplete)         :: 87 of 181
            Pseudo Genes (internal stop)      :: 72 of 181
            Pseudo Genes (multiple problems)  :: 53 of 181
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:47:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 606182
            Orig. end                         :: 624137
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..17955
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..17955
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17955
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:12955]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..12955
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(351..662)
                     /locus_tag="OTU45_RS03030"
                     /old_locus_tag="OTU45_03030"
     CDS             complement(351..662)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261043.1"
                     /locus_tag="OTU45_RS03030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03030"
                     /product="helicase BlpT"
                     /protein_id="WP_267750036.1"
                     /transl_table=11
                     /translation="MTDTDPIKRAQTLITDLNKAYQACKQATADDVRFQEQLNSILGFL
                     AKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKSRPS"
     misc_feature    801..814
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.71"
                     /tool="antismash"
     gene            939..1277
                     /locus_tag="OTU45_RS03035"
                     /old_locus_tag="OTU45_03035"
     CDS             939..1277
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /locus_tag="OTU45_RS03035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03035"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_047499348.1"
                     /transl_table=11
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNML
                     QNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYREIR
                     QKWLKEGE"
     gene            1282..2019
                     /locus_tag="OTU45_RS03040"
                     /old_locus_tag="OTU45_03040"
     CDS             1282..2019
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /locus_tag="OTU45_RS03040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03040"
                     /product="response regulator transcription factor"
                     /protein_id="WP_050116081.1"
                     /transl_table=11
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLLA
                     EVHEKGDHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQVS
                     ALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYYLET
                     SPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKLLFFP
                     NGGSCLIARYKVREVSEAINNLH"
     gene            2033..3373
                     /locus_tag="OTU45_RS03045"
                     /old_locus_tag="OTU45_03045"
     CDS             2033..3373
                     /EC_number="2.7.13.3"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /locus_tag="OTU45_RS03045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03045"
                     /product="sensor histidine kinase"
                     /protein_id="WP_001017642.1"
                     /transl_table=11
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILLA
                     FVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRGVS
                     YFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQKSLT
                     QINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKEKLYE
                     RLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIKEVYGS
                     VLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEIQVEGMS
                     LLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGIDISEIFSF
                     GASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(3444..3572)
                     /gene="blpC"
                     /locus_tag="OTU45_RS03050"
                     /old_locus_tag="OTU45_03050"
     CDS             complement(3444..3572)
                     /codon_start=1
                     /gene="blpC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /locus_tag="OTU45_RS03050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03050"
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="WP_000358815.1"
                     /transl_table=11
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(3629..4990)
                     /locus_tag="OTU45_RS03055"
                     /old_locus_tag="OTU45_03055"
     CDS             complement(3629..4990)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /locus_tag="OTU45_RS03055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03055"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_267750037.1"
                     /transl_table=11
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKEI
                     TVTSQEEIAPTSVIASIQSTSDNPILANYLVANQVVEKGDLLIKYSETMEESQKTALAT
                     QLQRLEKQKEGLGILKQSLETTTDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTNTEV
                     SNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRYLVAS
                     QGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVLRTQFL
                     QTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRIPTGTEI
                     AQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQTIDQTPT
                     RTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILTHSD"
     gene            complement(5001..>6179)
                     /gene="blpA"
                     /locus_tag="OTU45_RS03060"
                     /old_locus_tag="OTU45_03060"
                     /pseudo=""
     CDS             complement(5001..>6179)
                     /codon_start=1
                     /gene="blpA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /locus_tag="OTU45_RS03060"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03060"
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /pseudo=""
                     /sec_met_domain="TIGR01193 (E-value: 9.7e-181, bitscore:
                     599.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPFEKMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNI
                     DSEFVDYLEKSFKLSKYSILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNT
                     LFSYFTTPMENIINLQTKLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDD
                     LSYKYGFGRDTLTDINLTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQD
                     IKNIDKKVLRRHINYLPQQAYIFNGSILENLTLGGNHMISQEDILKACELAEIRQDIER
                     MPMGYQTQLSDGAGLSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLIS
                     LTDKTILFVAHRLSIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            complement(6200..7153)
                     /locus_tag="OTU45_RS03065"
                     /old_locus_tag="OTU45_03065"
     CDS             complement(6200..7153)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /locus_tag="OTU45_RS03065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03065"
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="WP_001155321.1"
                     /transl_table=11
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTIH
                     NEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQKF
                     SPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGKSIE
                     ERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVNQALT
                     LLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRRWLPKG
                     TKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     misc_feature    7124..7139
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.67"
                     /tool="antismash"
     gene            complement(7406..7867)
                     /locus_tag="OTU45_RS12020"
     CDS             complement(7406..7867)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="OTU45_RS12020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="WP_309543754.1"
                     /transl_table=11
                     /translation="MVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYILQQV
                     MSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSIIDALA
                     STILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPF"
     gene            complement(7812..8399)
                     /locus_tag="OTU45_RS12025"
     CDS             complement(7812..8399)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /locus_tag="OTU45_RS12025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_000205166.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-41, bitscore:
                     138.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKTN
                     KEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQTK
                     KDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPSSDF
                     QTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            8713..8910
                     /gene="blpI"
                     /locus_tag="OTU45_RS03075"
                     /old_locus_tag="OTU45_03075"
     CDS             8713..8910
                     /codon_start=1
                     /gene="blpI"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /locus_tag="OTU45_RS03075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03075"
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="WP_001093259.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.3e-10,
                     bitscore: 37.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAATG
                     PITGGAATLICVGSGIMSSL"
     gene            9377..9646
                     /gene="blpJ"
                     /locus_tag="OTU45_RS03080"
                     /old_locus_tag="OTU45_03080"
     CDS             9377..9646
                     /codon_start=1
                     /gene="blpJ"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /locus_tag="OTU45_RS03080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03080"
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="WP_001093248.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.3e-18,
                     bitscore: 63.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITGG
                     TGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            9715..9945
                     /gene="blpU"
                     /locus_tag="OTU45_RS03085"
                     /old_locus_tag="OTU45_03085"
     CDS             9715..9945
                     /codon_start=1
                     /gene="blpU"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /locus_tag="OTU45_RS03085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03085"
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="WP_000379967.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.7e-16,
                     bitscore: 56.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQL
                     GIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            9948..10067
                     /locus_tag="OTU45_RS03090"
                     /old_locus_tag="OTU45_03090"
     CDS             9948..10067
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /locus_tag="OTU45_RS03090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03090"
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346296.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     misc_feature    complement(10203..10216)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.08"
                     /tool="antismash"
     gene            10366..10530
                     /locus_tag="OTU45_RS03095"
                     /old_locus_tag="OTU45_03095"
     CDS             10366..10530
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /locus_tag="OTU45_RS03095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03095"
                     /product="hypothetical protein"
                     /protein_id="WP_000727118.1"
                     /transl_table=11
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYFA
                     INKKKGEKK"
     gene            10527..10931
                     /locus_tag="OTU45_RS03100"
                     /old_locus_tag="OTU45_03100"
     CDS             10527..10931
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /locus_tag="OTU45_RS03100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03100"
                     /product="hypothetical protein"
                     /protein_id="WP_000846944.1"
                     /transl_table=11
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQFL
                     LVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGE
                     SYQQLIAYIGAVLCLIIMLLLMWGLKYYKK"
     gene            11134..11939
                     /locus_tag="OTU45_RS03105"
                     /old_locus_tag="OTU45_03105"
                     /pseudo=""
     CDS             11134..11939
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /locus_tag="OTU45_RS03105"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="OTU45_03105"
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGGR
                     KPKLSLEDLLMATLQYV"
     gene            12089..12343
                     /gene="blpM"
                     /locus_tag="OTU45_RS03110"
                     /old_locus_tag="OTU45_03110"
     CDS             12089..12343
                     /codon_start=1
                     /gene="blpM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /locus_tag="OTU45_RS03110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03110"
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="WP_000379879.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 6.8e-19,
                     bitscore: 65.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLG
                     TAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            12359..12562
                     /gene="blpN"
                     /locus_tag="OTU45_RS03115"
                     /old_locus_tag="OTU45_03115"
     CDS             12359..12562
                     /codon_start=1
                     /gene="blpN"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /locus_tag="OTU45_RS03115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03115"
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="WP_001099492.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.8e-12,
                     bitscore: 43.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGPW
                     GAAIGGIGGAVVCGYLAYTATS"
     gene            12806..12955
                     /gene="blpO"
                     /locus_tag="OTU45_RS03120"
                     /old_locus_tag="OTU45_03120"
     CDS             12806..12955
                     /codon_start=1
                     /gene="blpO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /locus_tag="OTU45_RS03120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03120"
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="WP_001818346.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 5.8e-10,
                     bitscore: 36.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGC
                     ATTV"
     gene            13059..13178
                     /locus_tag="OTU45_RS03125"
                     /old_locus_tag="OTU45_03125"
     CDS             13059..13178
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /locus_tag="OTU45_RS03125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03125"
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346296.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     misc_feature    complement(13314..13327)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.08"
                     /tool="antismash"
     gene            13964..14347
                     /locus_tag="OTU45_RS03130"
                     /old_locus_tag="OTU45_03130"
     CDS             13964..14347
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /locus_tag="OTU45_RS03130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03130"
                     /product="hypothetical protein"
                     /protein_id="WP_000877381.1"
                     /transl_table=11
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGVF
                     LLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFI
                     VFNALMYLLSSSQVSLKKNKLSS"
     gene            14399..15088
                     /locus_tag="OTU45_RS03135"
                     /old_locus_tag="OTU45_03135"
     CDS             14399..15088
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /locus_tag="OTU45_RS03135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03135"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_050233598.1"
                     /sec_met_domain="Abi (E-value: 1.7e-12, bitscore: 44.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFVW
                     IQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKHVQ
                     STAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDLLLP
                     SILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIVATFPFLLT
                     FLHRVLG"
     gene            15130..15363
                     /gene="blpZ"
                     /locus_tag="OTU45_RS03140"
                     /old_locus_tag="OTU45_03140"
     CDS             15130..15363
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="OTU45_RS03140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03140"
                     /product="immunity protein BlpZ"
                     /protein_id="WP_000276498.1"
                     /transl_table=11
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFNF
                     LNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            15514..16125
                     /locus_tag="OTU45_RS03145"
                     /old_locus_tag="OTU45_03145"
     CDS             15514..16125
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="OTU45_RS03145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03145"
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_050202405.1"
                     /sec_met_domain="Abi (E-value: 8.2e-20, bitscore: 68.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSLL
                     VTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLIYIHFFAVSSGAGVQHLAE
                     VSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSNVPS
                     FIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            16287..17081
                     /locus_tag="OTU45_RS03150"
                     /old_locus_tag="OTU45_03150"
     CDS             16287..17081
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="OTU45_RS03150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03150"
                     /product="phosphotransferase family protein"
                     /protein_id="WP_000363002.1"
                     /sec_met_domain="APH (E-value: 4.6e-18, bitscore: 63.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTVLSKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            17078..17713
                     /gene="trmB"
                     /locus_tag="OTU45_RS03155"
                     /old_locus_tag="OTU45_03155"
     CDS             17078..17713
                     /EC_number="2.1.1.33"
                     /GO_function="GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="trmB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /locus_tag="OTU45_RS03155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_03155"
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="WP_001266083.1"
                     /transl_table=11
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVEV
                     GSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTDYF
                     EDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFEYSL
                     VSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
ORIGIN
        1 tcgaggattg accagttttg ttttacttgt ggaattagtt gttggcttga ttaccttcca
       61 agaaaaacta gcccttctag ccttactagg agctggtttg gttttactag tcttgtattt
      121 gccttatcag gtaaaacgtc agatgcagga ctaacattgc tgatacgaca ctaaaaaaga
      181 agttgagttc agtctgtctc aacttctttt ttgttactac aggataatgg ttggtccgta
      241 gagacttata ctcttcgaaa atctcttcaa accacgtcag cgtcgtctta ccgtactcaa
      301 gtacagcttg cggctagctt cctagtttgc tctttgattt tcattgagta ttaacttggt
      361 cttgacttgg tcttgacttg gtcaaagtgg aagcggtcat aggcccgcca agcggcgcga
      421 gttggagcat ctggatcaag agcgctgagt cccatgagaa gactggaagt ctggtaaaat
      481 ttttccaatt caatcaagaa tcgattatcc actgtttcag ccttggctag aaaaccaaga
      541 atagagttta attgctcctg aaagcggacg tcgtcagcgg ttgcctgttt gcatgcttga
      601 taggctttgt ttaagtcagt aatcaaagtc tgagctcttt tgatagggtc tgtatctgtc
      661 atgagaattc ctcctctaat ctgggtgcca gtcttgattc tagcaactgt gttttgatac
      721 tgtcagtcta tcacttttat ctcctttttc gcctttaaat ctttaattgt cattgaaatg
      781 tcttgaattg cgctgagtga attttatgat aaaatagttg taagctcatc atgatgttgt
      841 agaaaaataa tccttttagg agctttcaaa gactgtttag gattgggtgg gcttgggcta
      901 gaccttttct gttattcttt tcttaggagg agaatccaat gaaacatatg attattcaga
      961 cgcagaaaac agtctataaa gtaaacgtcg acgatatcta ctatatccaa acacatccaa
     1021 ctaaagccca taccgtacag attgttacag aagaagctag ttttaatatg cttcaaaatt
     1081 taagtaatct tgagaaccaa tgtggggaaa ccttgatgag atgtcatcga aattgtttgg
     1141 ttaatcttga taaattaaaa tcgattgatt ttcaagaaag aatccttttt cttggagaag
     1201 aaggtcaata cgctgtcaag tatgccagac gtcgctatag agaaattcgt caaaaatggt
     1261 tgaaagaggg agagtaagaa gatgagaata tttgttttag aggatgattt ttcccaacag
     1321 actagaattg aaacgacgat tgagaaactt ttgaaagcac atcatatcat tcctagctct
     1381 tttgaggtat ttggcaagcc ggaccaactg ctggctgaag tgcatgagaa gggggaccat
     1441 cagctattct ttttggatat tgagattcga aatgaagaga tgaagggact ggaagtggct
     1501 agaaagattc gggatcggga tccttatgcc ctgattgtct ttgtgacgac tcactcggag
     1561 tttatgcccc tgtcttttcg ctaccaagtg tctgctttgg actacattga taaggccttg
     1621 tcagcagagg agtttgaatc tcggatcgag acagccctcc tctatgccaa tagtcaagat
     1681 agtaaaagtc tggcggaaga ttgcttttac tttaaatcaa aatttgccca atttcagtat
     1741 ccttttaaag aggtttacta tctcgaaacg tcgcccagag cccatcgtgt tattctctat
     1801 accaagacag acaggctgga atttacagcg agtttagagg aggttttcaa gcaggagccc
     1861 cgtctcttgc agtgccaccg ctcttttctc atcaatcctg caaatgtggt gcatttggat
     1921 aagaaagaaa aactgctttt ctttcccaat ggtggaagtt gtctaatcgc ccgttataag
     1981 gtcagggaag tgtctgaggc tattaataac ttacactgag ctaggagaat ttatgaacat
     2041 tgcttggata ttgttgtata cacttgttac taatggacta gaaattgtca tcttctttaa
     2101 ggtggatgga attgatctta ctttcgagag gatttttaag gcctttcttt ttaagatact
     2161 gttggccttt gtttttgtga tgattagcta tatagtagga aatgtttacc tatcttattt
     2221 tatggaaccc ttgtacggca taggcttatc tttcttatta ttgagagggc ttcctaaaaa
     2281 actccttttc ttttatggtc tctttccaat gatattggtg aatctctttt atagaggagt
     2341 ctcctatttt gtgcttccat ttttgggaca agggcaagta tatgatggct actcatttac
     2401 tgggttatgt ataataattt tcaatttctt catttctcta gcttttttga aatggttgga
     2461 ctacgatttt actagcttga gaaaggagat tctcgataaa gcctttcaaa agtccctgac
     2521 tcagattaac tggataatgg gtggctacta tctagtcatg gaaagtctgt ctttttttga
     2581 atatgaacaa agtattcaat caaagactgt tcgccatctc atcctagtct tttacctact
     2641 cttttttatg ggggttatca agaaattgga tacctatttg aaggaaaaac tctatgagag
     2701 attggaacaa gagcaggccc tgcgctatag agatatggaa cgctatagtc ggcatataga
     2761 ggagctttac aaggaagtac ggagttttcg ccatgattat accaacctct tgactagctt
     2821 acgtctgggc attgaagagg aggatatgga gcagataaaa gaggtctatg gctccgtctt
     2881 aaaggattcc agtcagaaat tgcagaacaa taaatatgac ctgggcagat tggtaaatat
     2941 tcgtgataaa gccctcaaaa gtcttctagc agggaaattt ttaaaagcca gagataagaa
     3001 cattgtcttt aatgtcgaag ttcctgagga gattcaggtc gaggggatga gtctacttga
     3061 ttttctaacc attgtgtcta tcctttgtga caatgctatt gaagccagtg tggaagctag
     3121 tcaacctcat gtttcaatcg cctttttaaa aaatggagca caggagacct ttatcatcga
     3181 aaactccatc aaagaagagg gcatcgatat ttctgaaatc ttctcctttg gagccagttc
     3241 taaaggggag gagagaggag ttggtctcta taccgtcatg aagattgtgg aaagccatcc
     3301 taataccagt ctaaatacca cctgtcaaaa tcaagtcttt cgtcaggtac ttactgtgat
     3361 acatacagaa tgatgaaaaa taagaccgag agtacttgtt tctcggtctt atttttttat
     3421 agttgtattg gatgatgaag ttcttatgtg atgtaatgag catatctatt aatgttatat
     3481 aataaatctt cccacaatcc tccacctgta atttggttga gttcggtagt tgttagttct
     3541 tgaaatgaag ttaggttttg tttcttatcc atgttatgat tctccttttt gataagataa
     3601 taaatagtta tagagtgtta tctgaaaatt aatcagaatg tgttaaaatt ttatctttga
     3661 aataatcaaa atatgttttc tttgtagtta cactagtgac gcgaccttgt aagccatatt
     3721 ggatgagttt actatcctcg ttagatagtt ttgcaagagc ggttaattta aagagatttc
     3781 cttgctctgt tctggtagga gtttgatcaa ttgtctgaag ttggccgatg atggtggtgc
     3841 cgtgatttcc aatcttctcc agttttaatc ttacagtttg tcctttatct agtagaggta
     3901 gatagtcaga agatacgtag taagtgatta gtacttctct tgtatctgtg atgacaggga
     3961 atatttgagc aatttctgta ccagttggaa ttctattttt accttcaaat tcgctgttca
     4021 gatgaacgat acctttactt ggggaggtta aggtattgtt ttccaaacgc tgtgtggctt
     4081 gatctagttg tacttttaat tctgttaatt gattctccac agttagttgt tgctgtgagg
     4141 ctgtctgtaa aaactgagtg cggagtactt caattttggt tgctaaactg ttatcataag
     4201 ttgctacact tccgatacca gcttgctgaa ttttgaggct tgcgatagat gattcaagac
     4261 ctgcaatact ttgattaatt tgagataaaa atggctcctc tgcagttcct tgtgtttgtc
     4321 cttgtgaggc tacaagataa cgattcaaaa ttgactggtg cggattgcca gttggtaagc
     4381 gtgctctgtt atttatgata gcatctctca actcttgata ttctccaatt tgttgttgaa
     4441 cttttgtaat ttcttgttca atagctgatg aactgctatt ggaaagatta gcttgatttg
     4501 aaacttcggt gttagtcttt gtgataccca gttcaatatc atgggattgt ttagtaaaat
     4561 tcataaaggt attatggtag ccaaattcat cctcgccaga aaaaagatca gtcgttgttt
     4621 ctaagctttg tttcaaaatt ccaagtcctt ctttttgctt ctcaagtctt tgtaattgag
     4681 ttgctaaggc agttttctga ctttcttcca ttgtttcaga gtatttgatg agtaagtcac
     4741 ctttctcaac tacttgattt gccactaaat aattagcaag gataggatta tcactggtcg
     4801 actgaataga tgcaatgaca cttgtagggg cgatttcttc ttgggaagta acagtaattt
     4861 cttttgtggc aacaagggag aaaatcaaga tgaaagtaaa cagtaatgaa agaggtataa
     4921 ttaacactgt cgcatagtta tggtaacgtc tctgataaaa ctcgacgctt ctaaaaagat
     4981 taggattcat gacattctcc ttatttattg aatagatgat ggtagaagcc ttgcgcctgc
     5041 attaactctt ggtgactacc aacttcaatg attttcccct ggtcaagaac aatgacacgg
     5101 ttggttcgtt cggctatact gagacgatgg gctacaaaga gaatggtttt atcagttagg
     5161 gatataagat tatctataac ctttttctca gtcaagacat caagaccgct agtagcttca
     5221 tctagtatta aaacaggagc tttagttaaa agagcacgag cgagagcgat tcgttgcttt
     5281 tgtcctcctg atagaccagc tccatcagag agctgagttt gatagcccat aggcattctt
     5341 tcaatgtctt gacgaatttc agctaattca caagctttta gaatatcttc ttggctaatc
     5401 atatgattac cgcccaaggt taagttttcc aaaatagagc cattaaagat ataggcttgt
     5461 tggggtaggt aattaatatg acggcgcaag acttttttat caatgttttt aatatcctga
     5521 tgattgatgg aaatatgccc tttgtagggt tcaaagaaat tgacaatcat tttggctaaa
     5581 gttgttttac cagaaccact aactccaact aggctaacct tatctccttg tttaatcgtg
     5641 agattaatat ctgttaaggt atctcgtcca aaaccatact tataagaaag gtcatcaaat
     5701 tcaatatcgc ccatcaaaaa atgtgaatga acagggtttt cttgaacttg aaattcagat
     5761 tcgactagat agacttcgtt caaacggtta ttagcgacct tcgcagattg gagtttggtt
     5821 tggaggttga taatattttc cataggagtt gtaaagtaag aaaaaagtgt gttaaaggta
     5881 atcagctgac cgatagaaat tttactcgac atgactaatt gagcgccaaa ccataggata
     5941 aggatattca gaactaattt tgttccctgc tttaaactcg tttgtaaaat agaatattta
     6001 ctgagcttaa aggatttttc caaataatct acaaattcgc tgtctatatt ttgatagcga
     6061 ttttcttcac tcgtgagcga ctttatagtt tcaatcccgt tgatatcttc gataatggca
     6121 gagctaacca tagaattact ttgcatgaca tcatggttca ttttttcaaa aggtttcata
     6181 cccaaatttc aagttgaagt tagccaccca aaagaaattc acttggcgac ttgtagtcca
     6241 agcatttttt agggtagttg ttaatccaat tttcgataaa agctacttct ttcggagtcg
     6301 ttttcttggt tcctttaggt aaccatctcc gaattaatcg attatgattt tcatttgaac
     6361 ctctctccca tgaagagtaa gcatgtgcgt agtagatatg ttcctcagga aatacctcag
     6421 acaatcgttt gaactccgaa ccattatctg cagtaatgga cagaatacga tgctccccta
     6481 atagtaacgt aagcgcctga ttgacagatt cagcagtttt atttggaatt aaccttataa
     6541 tttggtgtct gctccgccgg tcggttaaga ctaacaggca ataatttttt atcttagtca
     6601 gtaggacggt atcaatttca taatgaccat tttccaagcg aagattaata acgtcaggac
     6661 gttcttcgat agatttacct gccggcttaa agttcggact agcttgcttc ttgacacctt
     6721 tcctttttct gggataaagc atgtcggcct tcgtcaatcc taaatgacca ttatgaaacc
     6781 agtagtagat ggttgaaata ccaactttca cttgcttctt gttaaccatc atttcaggcg
     6841 aaaatttttg cttatgatag tgtaagatct tctctctgat ttcctttgtt aaaattaact
     6901 ttttcaccga ccgttttcga ttgaattggt aaacagtttg tgcgtaatcg gcagaataga
     6961 cctttttgta tagccctttt ctcacttgtt gtaaagttgt acctcttttg acttcattat
     7021 gaatcgtttg aggcgccttt cctaacaagc ctgcaatttc acgattggac ttattttcat
     7081 tcttccaccg ctcaatcaag cgacggttat ctattgtcaa atgtttccct tttggggtat
     7141 aatgttcttg catctctgtg cctttccttg tgtttgtggt gaacaacaag tatagcacag
     7201 aggtgttttc ttatacctta aatcacattt catttgataa caggtttgaa cctgtttttt
     7261 cagctaaacc aaggctatga ttagccttgg ccacctagcc taacagtttc aaacctgtta
     7321 tcaaatgaaa tcatcaactt actcaaacat agtgaaacat ctaattcttg actaaggtaa
     7381 ggtggctaac ttcattatag aactttcaaa aaggtttcat aaaagaaaag atgatgaaca
     7441 tgtatatagg aatggaaaga agagaaagga ggaagagatt agggttttgt gccagcaaga
     7501 ctcctcctac aagaatcaga atagaaacat ccagaaaaag agaaagaatg gtagaagcca
     7561 aggcatctat aatagagtta gcatctgtga accgtgaaat gatttctcct gtacgacgtg
     7621 tcgcaaagaa agacatggga agttcaaaaa tatggcgaat ataggataaa atcacatcaa
     7681 tgcttaatct ctggctcaga acggttagga gataatctct ggaaaagctc atgacttgtt
     7741 ggaggatata ggtgataacc agaccaactg agatgattcc taaagttgat ttcatctgat
     7801 ttgggatgta ttcatccaag attccttgga gataataaga accaccgata ttgataatag
     7861 tgaccaataa gcttgagaga acaatgtaag caatgagaga tttttgtttg aaaatcagag
     7921 gaaggaagct tgcttagtag accattcttt ttatctttat ggggttgata gctgggtttg
     7981 gtagctagaa aaatagctac tccagtccat tcatagaaaa agcgttcttt tgacatttta
     8041 gtgattttta cagaagggtc aggatcacca ataatcagat agtctttctt tgtttgatag
     8101 acaacatagt aatgttggag ttttccttct ttgttaacgt gaacgataaa tggatagggg
     8161 acatcactca tgtcaaagag cgttttatct gcttgaacag gtcttgtttc aaagcccatt
     8221 tcatcagcgg cttttacaat gccaagagca gtcgtccctt ctttattggt ctttgcaagt
     8281 tctctcaagt gagctagaga aaaatctgaa ccatagaatt tagcaatcga ggctaaggca
     8341 gcgacaccac agtctctcgc atctatttga ggaacaaatg tacgtttata agaagtcatt
     8401 ggcaattcct ttcatatagt ggataggtct attatgtcat aaaactaagt tcatatttta
     8461 tgaaatcttg aatagtcatt aaaacttcct gaatggtaaa aaagtgatta gaaattattt
     8521 tttttaaaca tttagaggtg gcttgaaata gaggtggctt gaaatagagg tggcttgaaa
     8581 taaaaaagct aattcaagac gtttcgatga caattcaaga tctggatgaa aaattttaaa
     8641 aaacgatgat atactaaact tatcaaagtt acaacaagac aaaaataaaa aaagaaaaaa
     8701 ggagtatttg tcatgaatac aaaaatgatg gaacaatttt ctgttatgga taatgaagaa
     8761 ctcgaaatag ttagtggagg aagaggcaat ttaggatctg caatcggtgg ttgtattgga
     8821 gcagtactat tagctgctgc gactggtccg ataactgggg gagcagcaac acttatttgt
     8881 gtaggttcag gaattatgtc ctctttgtaa gattatgatt cttaaatata gtattattat
     8941 cgctattaat ttactgtctt atttacttac ttacaaaata tcaaagttat ctaagaatga
     9001 tgagaataag atagttagca aaattttaat tatattatca atcgtttatg taatcgttga
     9061 tgctttactt agttgatgga ttggtaggaa taaaaaactt aactataaac tattatttac
     9121 taatattttt tgctttctgc aagttaataa atagtatagt aaaacttttt attgcaagga
     9181 gaataacgaa attaagatat tttagatact caagattatt gaaatcttag actaaaaaat
     9241 aggtactgct acctttaaga agatagtaga cgtatatact tttttaagaa aatcaaaaag
     9301 atactataaa aaatctattt gtttattgaa tttaagactt tggtaacaaa ttgaaaataa
     9361 aaaggaggta ttcatcatga atacaaaaat gttgtcacag ttagaggtta tggatactga
     9421 aatgcttgcg aaagttgaag gggggtatag ctccactgat tgtcaaaatg cactgattac
     9481 aggagtcact acgggaatta taactggtgg aactggagca ggtttggcca ctttaggagt
     9541 agctggactt gctggtgcat ttgttggagc acacattggt gcaattgggg gtggattaac
     9601 atgcttagga ggtatggttg gtgataaatt gggactaagt tggtaagact tatagtaaaa
     9661 ttatgaggat tctattctga tttattttag ttttttaaaa aaggagtatt tgtcatggat
     9721 acaaaaatga tgtcacaatt ttctgttatg gatactgaaa tgcttgcttg cgttgaaggt
     9781 ggcggatgca attggggaga ttttgccaaa gcaggtgttg gaggaggagc agcaagaggt
     9841 cttcaactag gcattaaaac aggaacatgg caaggtgcag caactggtgc tgcgggtgga
     9901 gctatacttg gaggtgtagc ctatgcagca acatgttggt ggtaattatg gattttaaaa
     9961 gttttattat tggtttagta gttggtatat ttggtcctta tatggatgac ttaattagaa
    10021 aaatattttc aaaatcttcc aaaaaggata cggatagtac tctctaaaaa ataaatttct
    10081 cccctgtatt tgctagctta atttacaggg gagtttcttt aatattgttt cgcctgtcaa
    10141 aatgatatgt gatagcagga tgaaaagtct gcaaacaagt tggtatcaat acaatataaa
    10201 gaggaaagta tgtttttaat gcttactttt taaatttttt aacaattcaa gaggttttga
    10261 tgaccattta tgatttgagt gatccaaggt caaaataagt gctatactag cagtgtaaat
    10321 gttcttgctc aactaagata aatcgcacat ttaggaggaa atcttatgaa gaaaaaaata
    10381 ctgattattt tcgttctgta tctaatcatg tccatctttc tatatccgct tagggagagt
    10441 gcttggtatc agctatttta taccatagcc tatgtgattg cagttatgat ctattttgct
    10501 atcaataaaa agaaaggaga aaagaaatga aaacttttct tgctaaaaaa cggaacatct
    10561 tccttgcgag attgttctta ggtcagttgc ccttgcttgt ttctacttat ctatttctat
    10621 ctcgtcagtt tttaaatttt tccgtagttt tccaatttct tttagtagtt attaacttgg
    10681 cttctatttt ggttactgtt tatctcacta gggaaatgag gataagagag tttgaagatg
    10741 atgatttggt tagtcctaga accaatcaac ttatgtttat cggcttgaca ggttttatgt
    10801 ctattatttg tttgtataga ggtatcacag caggagaatc ttatcaacaa ctaatcgctt
    10861 atattggcgc tgttctctgc ttaatcatca tgcttctgct catgtggggc ttgaagtatt
    10921 ataaaaagta gagcttaatg aagtataaaa gaaaagggta agtgtttttg caatacttac
    10981 ccttttataa tagattccct gcgaaacaaa atatggtata gtatagtata ttgaaactag
    11041 aatagtacgc ctctacttct aaaacattgt tagaaatcga tttgactgtc ctgatcgatt
    11101 tatcctgttc ttatttcatt ttactatagt tctatgaatt atgaagcaag taaacaacta
    11161 actgatgcac gatttaagcg tcttgttggt gttcagcgta ccacttttga agagatgtta
    11221 gctgtattaa aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta
    11281 agcctagaag accttcttat ggccactctt caatatgtgt gagaagaccg cacttatgaa
    11341 gaaattgcgg ctgatcttgg tattcacgaa agcaacttaa tccgtcggag ccaatgggtt
    11401 gaagtaactc ttgttcaaag tggtgttacg atttcaaaaa ctcatcttag tgctgaggat
    11461 acggtgattg tggatgcaac agaggtaaaa ataaatcgtc ctaaaaaaat caactagcca
    11521 attattctgg taaaaagaaa tttcacgcta tgaaggctca agcgattgtc acaagtcaag
    11581 ggagaattgt ttctttggat atcactgtga actattgcca cgatatgaag ttgttcaaaa
    11641 tgagtcgcag aaatatcgga caaactggta aaaccttggc tgacagtaat tatcaagggc
    11701 tcatgaagat atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aaaccgctaa
    11761 cagttaaaga taaagcctat aaccatgcgt tatctaagga gagaagcaag gttgagagca
    11821 tccttgccaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac
    11881 gcttcggatt acgaatgaat ttgattgctg gcattatcaa tcatgaacta ggattctagt
    11941 tttgcaggaa gtctaatgta atttttgcaa ttcaagacgt ttcgatgact attcaagatc
    12001 tggatgaaaa attttaaaaa acgatgatat actaaacttg tcaaagttac aacaagacaa
    12061 aaattaaaaa taaaaaagag tatttgtcat ggatacaaaa attatggaac aatttcatga
    12121 aatggatata actatgttat ctagtattga aggaggcaag aataattggc aaactaatgt
    12181 cttagaaggt ggtggtgctg cttttggtgg ctgggggcta gggacagcta tttgtgctgc
    12241 gagtggtgtt ggagcaccat ttatgggagc atgtggatac ataggagcta aatttggtgt
    12301 ggatctttgg gcaggagtaa caggggcaac gggtggattt taataaaagg agacttgtat
    12361 gaatacttat tgtaatataa atgaaacaat gctgagtgag gtttatggag gtaattccgg
    12421 aggagcagct gtagttgctg ctttaggttg tgcagcgggt ggagtgaaat atgggagact
    12481 tctaggacca tggggcgctg caataggagg aattggagga gcagtggttt gtggatattt
    12541 agcctatacc gctacatcat aaaaatgaaa gctaaatttt atgaaggcta tattctttat
    12601 tattctcttc gcttttcaaa cctatcttat ttatctatcc ataagtatct ccgataaaaa
    12661 acaaaagact attgaattaa catcattgaa ttgttttgtc atattatttc taatctatga
    12721 taaattaatt tttctcttta ttgcctatgt ttttttgata atttttatat taaacttgtt
    12781 ccgtaactga aaagtgatat actatatgga tacaaaaatg atgtcacaat ttgcagttat
    12841 ggataatgaa atgcttgctt gcgttgaagg tggagatatt gattggggaa gaaaaattag
    12901 ttgtgcagca ggggttgcat atggcgcaat tgatgggtgt gcaacaacgg tttgacattt
    12961 ctattgggac catttgctat aggaataggt gtaactggtg ctgcaggtgg agctatactt
    13021 ggaggtgtag cctatgcagc aatatgttgg tggtaattat ggattttaaa agttttatta
    13081 ttggtttagt agttggtata tttggtcctt atatggatga cttaattaga aaaatatttt
    13141 caaaatcttc caaaaaggat acggatagta ctctctaaaa aataaatttc tcccctgtat
    13201 ttgctagctt aatttacagg ggagtttctt taatattgtt tcgcctgtca aaatgatatg
    13261 tgatagcagg atgaaaagtc tacaaacaag ttggtatcaa tacaatataa agaggaaagt
    13321 atgtttttaa tgcttacttt ttaaattttt taacaattca agatgtttcg atgacaattc
    13381 aagatttgga tgaaaaattt taaaaaacaa tgatatacta aacttgtcaa agttgcaacg
    13441 ggtgctgcta cattatgtta aaaatcgctc ttgattgata agctggattt gttaggaaag
    13501 gagtaaataa ttatggataa gaaaaagata gtttcgacta taatatgtat agtatttctt
    13561 gtagtgtcag tagataattt ttttagagac ttaaccccgt tactgttcat tttaaatatt
    13621 attggcctgt cttgcttttc agtgctaaca tacattaaca taaaagagat actcttaaat
    13681 ataagtaaat gatgatttgg ttagtcctag aaccaatcaa cttatgttta tcggcttggc
    13741 agattttatg tttgtcattt gtttgtatag aggtatttca gaaacagaat tctatcaaca
    13801 actaatcgct tatataggtg tattctctgc ttgtttatca cgcttctgct catgtggggc
    13861 ttgaagtatt ataaaaagta gctttctatc cttttctgac aggatatttg agtagaaggc
    13921 tctattaaat tgttagtgaa ataaatgaat ggaggtattt aatatgaaat acagattatt
    13981 ttttgttatt ttcttgagta gtgtgttgga tattctttta gggacatttt tacaaatctc
    14041 tatcgtatct attgggtggc ttgttcttta cagtggtttg tttgaagcag gagttttcct
    14101 tcttgctaat aaaggggtgg cggtaaaaat caaggaagta gatattcgaa atcgctttaa
    14161 atttattttt ggaaaaacct tatggtttca aattcttttg ctcatctttt tgataatcaa
    14221 actttatctt ggtttggatg cgaggttgat tttattctat ggacatattt tcattgtctt
    14281 taatgcctta atgtatctat tatctagtag tcaggttagc cttaaaaaga acaaactgtc
    14341 ttcttaatct tacagcagta gagcctgaca aggtgaggtc atagtaggaa gaatttctat
    14401 gaaaaagtat caacttctat tcaaaataag tgcagttttc tcttacttat ttttcgtatt
    14461 tggtctttct cagatgacgc ttattattca aaattattgg caattttctt cccagattgg
    14521 caatttcgtc tggattcaaa atttcttgag tttgctattt agcggagtca tgatttggat
    14581 tctggttaag acaggtcatg gttatctctt tcacattcca agaaaaaaat ggctttggta
    14641 ttcgattttg acagtattag tggtagtgct ccagatctct tttaacgttc agacagctaa
    14701 acatgttcag tcaactgctg aaggttgggc tgtattgatc ggttatagtg ggaccaactt
    14761 tgctgagcta ggtatctata taactttgtt ctttctgact ccacttatgg aagagctaat
    14821 ctatagagga ttactgcaac acgccttttt taagcattcg agatttggcc ttgatttgct
    14881 tcttccgtca attttatttg ctcttcctca tttttcaagc ctgcctagtt tgttagatat
    14941 cttcatcttt gcaacatctg gcatcatctt tgctagtttg acccgctata ccaagagtat
    15001 ttatccatcc tatgcggtgc atgtgatcaa taatattgta gcgaccttcc cgtttttgct
    15061 cacttttcta catagggtct tggggtaaaa aaacaaaagg cttgcttttc agccatagag
    15121 gaggtcatca tgtataaaca cttatttttc ctagattcca aaactttaga tcggttgaca
    15181 ccctatattc tagtcttggc ttctgacacc attgctttta atgtttttgt gctaaccttt
    15241 gtatctgcgg tggtttttaa tttcctaaat tccatgctag ctttaatggc tatattcata
    15301 ggggctggct atgtggtcgg attttggtta ctaatactca atgaaaatca aagagcaaac
    15361 taggaaacta gccgcaggct gtacttgagt acggcaaggc gacgttgacg tggtttgaat
    15421 ttgattttcg aagagtataa aatggtttgt tttggaaagg ctagaactaa agaatgacgt
    15481 gtaggaagtc tgtttgttga ggaggataat tttatggagt tttttgataa atttcatgcc
    15541 ttgtgttttg gatttttagt actaataatc gttattacag ttccttatac gattaaccat
    15601 ggggattttt ttcaaaatga atctgcattg attattgtaa gtcttctagt aacctctctg
    15661 agtgttgctt atgctagaaa gtttgaaatg atttcttttg ggatgttaag caagaaacaa
    15721 cttttgcttt tcattgcaat ctttcttcta agtgtgcttg agacgctgat ttatattcat
    15781 ttcttcgctg tttcttctgg cgcaggagtt caacacttgg cggaagtcag cagaggaatt
    15841 tccctgtctt tgattttgac tacctcagtt tttggcccca tccaggagga actcattttc
    15901 agaggacttc ttcaaggtgc ggtttttgac aattcttggt tagggcttgt gctaacttcc
    15961 tctctctttt ctttcatgca tggaccttct aatgtccctt cgtttatttt ttatctactt
    16021 gggggtttgt tgctgggctt tgcttataaa aagagccaaa acctatgggt ttctactcta
    16081 gttcatatgc tttacaacag ttggccactc ttatattatt tataaaaatc atgaaaaggt
    16141 aagtaggaga ttgtgcttac ctttttcttt ctataatgat aacccaaacc actccagagg
    16201 cttttctttg agaatcgggt tagagtggtt gacgaatagg ccaaaaacta gtagaatagt
    16261 aaggaaactt tatacggagg aaagaaatgg atttgggtga taatgagcta acactgactc
    16321 ccatacctgg gaaaagtggc aaggcttata tgggtagcta tcctgatggg aagcgcatct
    16381 ttgtaaaaat gaacacctct ccaatcctac ctggtctagc tagagaacaa attgctccac
    16441 aattattatg gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga
    16501 caggcaagat attgaccccc tatgatatga atcgtaagca aatcgtcaat attttaaccc
    16561 gtcttcatcg ctcacgtccg ttgatgacac aattgagtcg tttgggctat gccatggaaa
    16621 cacctgtaga tttactacag tcttggcaag aaacggctcc agatgctttg cgtaaaaatc
    16681 attttatcag tgaagtgatg gctgatttac gtcagactat tccaggattt agagaggacc
    16741 atgcgaccat tgtccatgga gatgtacgac atagtaattg gattgagaca gatagtggct
    16801 tgatttattt agtagattgg gattcggttc gcttgaccga tcgcatgttt gatgtggccc
    16861 atatgctctg ccattatatt tcagaacatc agtggaagga atggttgacc tactacggtt
    16921 acaagtacaa tcaaacggta ttaagtaaat tgtattggta tggtcaattg tcttatttga
    16981 gtcagatttc caagtattat atgaaccaag atttagaaaa tgtcaatcgg gagattcatg
    17041 gtttgcgtca tttccgagac aagtatggaa agagaagatg agagttagaa atcgtaaagg
    17101 ggcaacagaa ttactagagg caaatcccca gtatgtggtc ctcaatccct tggaagccaa
    17161 ggcaaaatgg cgggacttgt ttggcaatga taatcccatt catgtggaag ttggaagtgg
    17221 aaagggtgcc tttgtttcag gtatggccaa gcaaaaccct gacatcaact atatcgggat
    17281 tgatattcaa aagtctgttt tgagctacgc tttggacaag gtgcttgaag ttggagtgcc
    17341 taacatcaag ctcttgtggg tagatggttc tgacttaact gactattttg aagacggtga
    17401 gattgatcgc ttgtatctga acttttcaga tccatggccg aaaaaacgcc atgaaaagcg
    17461 tcgtttgacc tacaagacct tcttggatac cttcaaacgt atcttgcctg aaaatggaga
    17521 aattcatttc aagacggata accgtggctt gtttgagtac agtttagtga gcttttctca
    17581 atatggcatg aaactcaatg gtgtctggct agatttgcat gccagtgatt ttgaaggcaa
    17641 tgtcatgaca gaatacgagc aaaaattctc aaacaagggg caagttatct accgagttga
    17701 ggcagaattt taagagatag cctaaaatta ggctgtacaa gtgcttttgc tttacataag
    17761 ttggcaaacg tgctatactg atagtaagaa tatgaaaagt gaggcgggga aatatcttcg
    17821 cctcttgctt atgaggaggt ggacgcaatc gcaacaatcg tagaattagt cagagaagtt
    17881 gtagaacctg tcatagaagc tccttttgaa ctcgtggata tcgagtatgg aaagattggc
    17941 agtgacatga ttctc
//
