LOCUS       NZ_CP113114            10288 bp    DNA     circular CON 14-JAN-2024
DEFINITION  Streptococcus pneumoniae strain NP1 chromosome, complete genome.
ACCESSION   NZ_CP113114
VERSION     NZ_CP113114.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Genome sequencing and assembly of Streptococcus pneumoniae strain
            NP1 isolated from nasopharynx
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310574)
  AUTHORS   Sagar,V., Kaur,G. and Gupta,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2022) Community Medicine &SPH, Post Graduate
            Institute of Medical Education & Research, Chandigarh, Room No 529,
            Research Block B, PGIMER, Sector 12, Chandigarh, Chandigarh 160012,
            India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: MaSuRCA v. 3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 356.0x
            Sequencing Technology :: Illumina Novoseq 600; Nanopore GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 02:39:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,409
            CDSs (total)                      :: 2,335
            Genes (coding)                    :: 2,154
            CDSs (with protein)               :: 2,154
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 86 of 181
            Pseudo Genes (incomplete)         :: 87 of 181
            Pseudo Genes (internal stop)      :: 72 of 181
            Pseudo Genes (multiple problems)  :: 53 of 181
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:47:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 213759
            Orig. end                         :: 224047
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5288](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5288
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(800..1120)
                     /locus_tag="OTU45_RS01055"
                     /old_locus_tag="OTU45_01055"
     CDS             complement(800..1120)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OTU45_RS01055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="OTU45_01055"
                     /product="hypothetical protein"
                     /protein_id="WP_000692958.1"
                     /transl_table=11
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSSKHCFKVVDGTDEVSS
                     KHCFKVVDGTDEVSSKHCFEVVDRTDEVSNHTYGKATLTWFEEIFEEYKSLHNKTHITK
                     VV"
     gene            complement(1170..1802)
                     /locus_tag="OTU45_RS01060"
                     /old_locus_tag="OTU45_01060"
     CDS             complement(1170..1802)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /locus_tag="OTU45_RS01060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01060"
                     /product="HAD family hydrolase"
                     /protein_id="WP_000200892.1"
                     /sec_met_domain="HAD_2 (E-value: 1.5e-37, bitscore: 126.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIRG
                     FMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPLYI
                     TTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIIIGDT
                     KFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(1824..2696)
                     /gene="sdaAA"
                     /locus_tag="OTU45_RS01065"
                     /old_locus_tag="OTU45_01065"
     CDS             complement(1824..2696)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /locus_tag="OTU45_RS01065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01065"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="WP_000500038.1"
                     /transl_table=11
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMER
                     NLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHN
                     AKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGA
                     EGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVK
                     RNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGR
                     RLQKEIFGE"
     misc_feature    2680..2696
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.79"
                     /tool="antismash"
     gene            complement(2705..3376)
                     /gene="sdaAB"
                     /locus_tag="OTU45_RS01070"
                     /old_locus_tag="OTU45_01070"
     CDS             complement(2705..3376)
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /locus_tag="OTU45_RS01070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01070"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="WP_000838912.1"
                     /transl_table=11
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTE
                     VEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKDSN
                     APHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGM
                     IALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFF
                     K"
     misc_feature    3531..3547
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.23"
                     /tool="antismash"
     gene            3617..4204
                     /locus_tag="OTU45_RS01075"
                     /old_locus_tag="OTU45_01075"
     CDS             3617..4204
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /locus_tag="OTU45_RS01075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01075"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_000836442.1"
                     /transl_table=11
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLSE
                     IAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETVSA
                     PVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYS
                     AENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(4256..>4582)
                     /locus_tag="OTU45_RS01080"
                     /old_locus_tag="OTU45_01080"
                     /pseudo=""
     CDS             complement(4256..>4582)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /locus_tag="OTU45_RS01080"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01080"
                     /product="DNA-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYT
                     LLEDAKNKKSYDRLAICYVPIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     misc_feature    4661..4677
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.88"
                     /tool="antismash"
     misc_feature    4666..4679
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.93"
                     /tool="antismash"
     misc_feature    complement(4667..4680)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.42"
                     /tool="antismash"
     misc_feature    complement(4939..4955)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.57"
                     /tool="antismash"
     gene            5001..5288
                     /locus_tag="OTU45_RS01085"
                     /old_locus_tag="OTU45_01085"
     CDS             5001..5288
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /locus_tag="OTU45_RS01085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01085"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_001230164.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 6.2e-30,
                     bitscore: 100.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFGY
                     SDYLHSTRYHTATVRHGGRTSKDYAKPEAWAIASLTKIPPTGMEYFYGFE"
     gene            5340..7448
                     /locus_tag="OTU45_RS01090"
                     /old_locus_tag="OTU45_01090"
     CDS             5340..7448
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="OTU45_RS01090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01090"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000724257.1"
                     /transl_table=11
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDLY
                     LKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPMLWLSHF
                     LLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTALLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFYQDLVEKT
                     QAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTGIKKVILMP
                     ASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFPYSDKRKENL
                     SNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKLSDGNYLKFSS
                     IQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRDIITKKFMGWKL
                     VDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVLGLASRMEKRSLV
                     ELLKGGIL"
     gene            7445..8086
                     /locus_tag="OTU45_RS01095"
                     /old_locus_tag="OTU45_01095"
     CDS             7445..8086
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /locus_tag="OTU45_RS01095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01095"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_267749928.1"
                     /transl_table=11
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGALVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     misc_feature    complement(8193..8207)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.38"
                     /tool="antismash"
     gene            8293..9099
                     /locus_tag="OTU45_RS01100"
                     /old_locus_tag="OTU45_01100"
     CDS             8293..9099
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /locus_tag="OTU45_RS01100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01100"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_267749929.1"
                     /transl_table=11
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALN
                     PDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKADLA
                     ISGVSKTDERSKVFDFSVPYYTSKNKVIVKKSDLATYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQLDAVIFEEPVAKGFVENNPDLAIAEIEFDQEK
                     DDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            9116..>9508
                     /locus_tag="OTU45_RS01105"
                     /old_locus_tag="OTU45_01105"
                     /pseudo=""
     CDS             9116..>9508
                     /GO_function="GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269502.1"
                     /locus_tag="OTU45_RS01105"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01105"
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMNVGEGKDLDFI
                     HDKALKVGAVESYVIDVQDEFATDYVLVALQAHAYYEQKYPLVSALSRPLISKKLVEIA
                     HQTGATTIAHGCTGKGNDQVEYQIAVA"
     gene            complement(9825..>10151)
                     /locus_tag="OTU45_RS01110"
                     /old_locus_tag="OTU45_01110"
                     /pseudo=""
     CDS             complement(9825..>10151)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /locus_tag="OTU45_RS01110"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OTU45_01110"
                     /product="DNA-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KILQRLEVYKNYQRLYDLRIAILLNLSTLYLYNQDKNMCKQICYT
                     LLEDAKNKKSYDRLAICYVRIGICRDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     misc_feature    10230..10246
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.88"
                     /tool="antismash"
     misc_feature    10235..10248
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.93"
                     /tool="antismash"
     misc_feature    complement(10236..10249)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.42"
                     /tool="antismash"
ORIGIN
        1 gttgcaagtc tttgagcagt acaaacctgc tattgtttat catgcggcag cccacaagca
       61 tgttcctatg atggagcgca atccaaaaga agccttcaaa aacaatatcc gtggaactta
      121 caatgttgct agagccgttg atgaagccaa ggtgcctaag atggttatga tttcgacaga
      181 taaggcagtt aatccaccaa atgttatggg agcaaccaag cgcgtggcgg agttgattgt
      241 cactggcttt aaccaacgta gccaatcaac ctactgtgca gttcgttttg ggaatgttct
      301 tggtagccgt ggtagtgtca ttccagtctt tgaacgtcag attgctgaag gtgggcctgt
      361 aacggtgaca gacttccgca tgacccgtta ctttatgacc attccagaag ctagccgtct
      421 ggttatccat gctggtgctt atgccaaaga tggggaagtc tttatccttg atatgggcaa
      481 accagtcaag atttatgact tggccaagaa gatggtgctt ctaagtggcc acactgaaag
      541 tgaaattcca atcgttgaag ttggaatccg cccaggtgaa aaactctacg aagaactctt
      601 ggtatcaacc gaactcgttg acaatcaagt tatggataag attttcgttg gtaaggttaa
      661 tgtcatgcct ttagaatcca tcaatcaaaa gattggagag ttccgcactc tcagtggaga
      721 tgagttgaag caagctatta tcgcctttgc taatcaaaca acccacattg aataaaaaag
      781 aaaaacgcat agtatcaagt tacacaacct tggtaatatg cgttttatta tgtagagact
      841 tatactcttc gaaaatctct tcaaaccacg tcaacgtcgc cttgccgtag gtatggttac
      901 tgacttcgtc agttctatcc acaacctcaa aacagtgttt agagctgact tcgtcagttc
      961 catccacaac cttaaaacag tgttttgagc tgacttcgtc agttccatcc acaaccttaa
     1021 aacaatgttt tgagctgact tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc
     1081 agcccgcagc tagtttccta gtttgctctt tgattttcat tgagtattac ttcattttct
     1141 tctgaaatgg aattgttacc cagtctatgc tattgaaaat acgccaaaac ttctaagggt
     1201 ttgtgagcga tataatcagg ttgatagttt agtagatctg cttgctctcc aaatccccaa
     1261 gtgatggcca atttctgaat acctgtttct cgagctccca gcatatcaaa cttggtatct
     1321 ccgatgatga tggcttgttc tggtgctagt tgatgtgtct gcaaggcttg gtgaatgaca
     1381 tctgccttat ggggtgcttc agggctagaa ccataaatgc catcaaagaa atgatggatt
     1441 tccaagtttt ttgccatgtc ttgagcagta gatgtatcct ttgtcgtggt gatgtagagt
     1501 ggataactgc tcgataactc ctcaagcaag tctataatct gaggaaagag ttgagcttca
     1561 tagatgcctt ttgccttata gtaagaacga tatatctgca cggcttcaga aatttggtct
     1621 ttggacaggc aggtcgcaaa actactttcg agaggtggtc ccataaaacc acgaatagtt
     1681 ttggcatcag ggctaggcac ccccagctct ttaaaggtat aggtaaaggc attgtgaatc
     1741 ccgatagaac tatcaacgag ggttccatcc aaatcgaaaa aaatcgctgt gatagaggtc
     1801 atggtttctc ctatttgata agcttattct ccgaaaattt ctttttggag gcgacgacca
     1861 gtaggggtgg tagcgagtcc accttcagct gtttcacgaa aggcagttgg catgcttgct
     1921 cctacttggt acatggcatc gatcacttca tccacaggga ttttagattc gatacctgcc
     1981 aaggccatgt ctgctgcgat gaaagcaaag ctagctccca tggcattacg tttgacacag
     2041 ggaacttcga ccaaacctgc aacagggtca cagatgaggc ctagcatatt tttaatgaca
     2101 aaggcaatag cttgactggc ctgataaggt gttccacctg cagccagagt caaggcggca
     2161 gcactcatag cagaggctga accaacttca gcttgacacc caccctcagc acctgagatg
     2221 gaggcattgt ttgcgatgac tagtccaaag gcaccagcag caaagaggaa atccaattgt
     2281 tgctcgtggc tgaggtctaa tttttcaata gcagcagtga gaacggatgg cagacagcca
     2341 gcacttccag cggttggagt ggcacagacc aagcccattt tggcattgtg ttcattgact
     2401 gcgatggcat ttcgggcagc cgagagaatc gtataatctg acagagtttt tccgttttcg
     2461 atgtagtgat ccaatttggc agcatctcca cctgtcaggc cactacgaga tttattttca
     2521 ttgaggccaa gttggacaga ggctttcata acttccagat tgcgttccat gagaaggaag
     2581 acttcttcac gttcgcgacc ggtcaattca aactctgttg taatcatgag ttctgcgaca
     2641 tttccttgaa agtccagatc tgcttgctcg accaattctt tgatagaata aaacatgctt
     2701 cctcctattt aaagaaattg acattgtgga gatgagggat ttttcgaatt tcttcgatag
     2761 cctcatcaca gttgcgactg tcaacttcga taatcataat ggctttttca ccagcttttt
     2821 cacgagtgac attcatctgg gcgatattga taccatagcg ggaaagcgcc tctgtaacaa
     2881 gggcaatcat acctggaata tcttgatgaa cgatgatgat agtcggtgta ttcatattga
     2941 gagagacggc aaaaccattg agttcggtta cctgaatatt tcctccaccg atagaaatac
     3001 cagtcacgct gatggtcttg tgggcatttt taacagtaat tttagtggtg ttagggtgag
     3061 gggcattgct gtctttctga atggtccaga caatcttgat accacgcttg tgggcaattt
     3121 ccagactatt tggaatttca ggatcatctg tatccattcc taaaatacct gcaacaaggg
     3181 ctaggtctgt tccgtgacca cgataggtct tggcaaatga gttaaaaagt tggaattcaa
     3241 cttctgtcgg agtatcatca aaaatggaag agacaatctt cccaatacga acagcaccag
     3301 cggtatggct actagatggg ccaatcataa ctggtccgat gatatcaaag acagattgaa
     3361 aacgaagtga tttcatcagt ttccccttat aaaaattctt atctctatta tatcaaagaa
     3421 tgaggggctt ggctttaatt gtggatgaaa acctttctaa tacctcaaat agcataaaaa
     3481 tagtatcttt tatgacaaaa acaccttatt tagggaaata aaaaataatt ttgtaatatt
     3541 tctacataaa agtgtcaaga aacggtaata tttaaagggt atgatagaac tatagaaaga
     3601 aggagaattt tcgaatatga aatcaataac taaaaagatt aaagcaactc ttgcaggagt
     3661 agctgccttg tttgcagtat ttgctccatc atttgtatct gctcaagaat catcaactta
     3721 cactgttaaa gaaggtgata cactttcaga aatcgctgaa actcacaaca caacagtgga
     3781 aaaattggca gaaaacaacc acattgataa cattcatttg atttatgttg atcaagagtt
     3841 ggttatcgat ggccctgtag cgcctgttgc aacaccagcg ccagctactt atgcggcacc
     3901 agccgctcaa gatgaaactg tttcagctcc agtggcagaa actccagtag taagtgaaac
     3961 agttgtttca actgtaagcg gatctgaagc agaagccaaa gaatggatcg ctcaaaaaga
     4021 atcaggtggt agctatacag ctacaaatgg acgttatatc ggacgttacc aattaacaga
     4081 ttcatacctg aacggtgact actcagctga aaaccaagaa cgtgtagcag atgcctacgt
     4141 tgcaggacgt tacggttcat ggacagctgc taaaaacttc tggcttaaca atggctggta
     4201 ttaagaacta acgaacaaat aatatgtaaa gagtcgagga aatccctcgg cttttttatc
     4261 tttccttcgc ttggtaatag atctctactt cttttttgag atgagacagc atagaagttt
     4321 cctcggttag ctctaggagt gagaaacctt tttggataag tttcgcatca tccctacaaa
     4381 tcccaatagg gacatagcag atagccagcc tatcgtagct tttcttattc ttggcgtcct
     4441 ccagtaaagt gtagcagatt tgcttacaca tgtttttatc ttgattgtat agatagagtg
     4501 tggaaaggtt gagcaggatt gctattcgca agtcatataa atgttgatag tttttataga
     4561 cttctaagcg ttgcaagatt ttctcggatt attgacaaat aaacggacat tctgatagac
     4621 gattgcccta gcggcttagt gtaaacttta caaatttggc ttgtaatttt tttataaaat
     4681 tcacttcagt tacttgacag ttattatatt ttcacttatt atatagataa aaggatggga
     4741 taggagttta aaaatttgaa agcgctatcc atattgtttg ttggactgat tcttgagtaa
     4801 tagttagagt gtgcttaata ctactctggt tgttcaagga cctgatacag aagcgcacca
     4861 ttctcccggt taagagtaag ttcgttgtta aaaaatgaaa tgagatagaa ggaaggtgag
     4921 tccaatggac tacgatactt gttgataaat tactatagct gtttttacgg aacagctctc
     4981 gataatagga gaaataaatc atgagaaaat tagttaaaat tggtgttgca tctttaatgg
     5041 tatgtggtat acttgctact acaaatgctt tagcagtatg ggttgagggt ggtcaatgga
     5101 actatggagt aggttggaca ggaacttttg gatattctga ttacttacat tctactcgat
     5161 atcatacagc aactgttaga catgggggta gaacctctaa ggattatgca aaacctgagg
     5221 catgggctat agcttccctc accaagattc cgccaacagg aatggaatat ttctatggat
     5281 ttgagtaaaa ttcttccctc tcctatttgg agagggaaaa ctgtagtgga ggcactttta
     5341 tgaaaaaaat cagtaatttc tgtatgttac tcctgcttct gtgtaccact ttttttgttt
     5401 ttaatgtaaa ctatacacga gaagtggttc ggattcaaga aatgggaaag actgtagatt
     5461 ctttggattt gtatttgaaa gatattaacg aacctgcagc gtctgttctt cgattttttg
     5521 aggatgtatc aaaggagtat aaagtctcca tcatcaaaac agacagtggt gatgaggtgg
     5581 tcaagtctgg tgtttttgat aaagatacct tcccctacca agagtttggg atttcttctc
     5641 ttgattttac cacagatggt gaaggagtct atagtaataa agaaatttcc aataaacttg
     5701 gtacgattcc gacctttcta aaagccaaac ctattcagct tatgactttt caaacctata
     5761 tcaaggatac atctcgtagt ttaaatggtc gctatacgat aacttctaca caagagatgg
     5821 acaaggatag gattgtacag aaatggagcg attttttcaa gatagaccag gctaccttgc
     5881 tagagccgac ctacaaaagt gcagtggaag tcataaatcg agatttgctt ttatctgcca
     5941 ttgtttttgt cttggctatt ttgcttcttg tgttagtgac agtgtatcaa ccgatgatgg
     6001 agatgaaaag agttggggta caaaaattac ttggttttca agatagggct gttttagctg
     6061 atgttgtaaa aggcaacctt tacctcctcc taggtggggc tcttgtgatc aatctaggcg
     6121 tgtttttctt gcttgattat aagccaaaag atttgtttcc tatgctgtgg ttgtctcatt
     6181 ttttgctgtt gcagctttat ctctttatca gttggttgac ttacctctta atccaaaaaa
     6241 tgacaatcag ctctctgctg aaaggttttt catctttcaa atttggtctt atcttcaatt
     6301 atgtgatgaa aatagggaca actattttac tgacggcctt actgattggg gtgggcagaa
     6361 gtttagaaca agaaaacaaa gaacttgctt atcagcaaca gtgggtaagt caaggtaatt
     6421 acctgacctt agaaaccttc aaactcaatg ataatctgtg gcaagaagag ctagcagggt
     6481 cagggaaatc tacagattat ttctatcgat tttatcagga tttggtagaa aaaacgcagg
     6541 cgggctatgt gcagagtagc agtcttcctg taaaaaattt tgtccaatca gaacagattc
     6601 agcaatatca gttaacagat acggtggatg tttactatgc caatcgcaat tttctaaaga
     6661 gcaagggatt caagctacca gataccggta ttaaaaaagt tattttgatg ccagcaagta
     6721 cgaaaggtga agaagataaa aatcagctct tggggaagtt aattgccttt cattcgatga
     6781 agtatgaaga gcagcaaaaa cgaacgatag aggagatgga tgtcgagatt gcctattatg
     6841 aaggagattg gtcatttttc ccatatagtg ataagcgaaa ggaaaatctc tccaatccaa
     6901 ttattagctt ggtcaatgat tctgatatga tgtgggatga gaaagcctcc ctgtcaacaa
     6961 ctggcttaaa taatccgatt aaaattgaaa atacggttca acatcaaaaa gagattacag
     7021 agttagttga gaaattgtca gatggaaatt atttaaaatt ttcatctatt caagccattc
     7081 aacaagagaa agtggattct tatagagatg ctgttcggaa ttttaaccta ctctttgctt
     7141 tgtttggtct ccttagcatg atgatttcct acttcttact agtaacaact ttcttattga
     7201 agcgcaggga tatcattacc aagaagttta tggggtggaa actggtcgat cgctaccgtc
     7261 ctctcctcgt tctgctcttg ctgggctata gtttccctct tctagtcttg attttctttg
     7321 ctcatgcgtt cttaccactt ctactgtttg caggttttac atgtctggat atactatttg
     7381 tgctaggctt agcttctagg atggagaaaa gaagtctagt agagttattg aaagggggca
     7441 tcttatgatt gagttgaaaa atattaccaa aaccattggg ggaaaagtga ttttggataa
     7501 cttatctctc aggattgatc agggggcttt ggtagccatt gttggcaaaa gtggcagtgg
     7561 caagtcgacc ttgttaaatt tattgggttt gatagatggt gattatagcg gacggtatga
     7621 gatttttggt cagacaaatc tagcggttaa ttctgctaag tcgcaaacaa taatccgtga
     7681 acatatctct tatctgtttc aaaattttgc cctgattgat gatgaaacgg tcgagtacaa
     7741 tctcatgctg gcgctgaaat atgtgaaatt gcctaagaaa gacaagctca aaaaggtgga
     7801 agagatttta gagagagtag gtttgtcagc tactttgcat caaagggtct ccgagttgtc
     7861 tgggggcgaa caacaacgaa ttgcagttgc tagagccatc ttaaaaccca gccagctgat
     7921 tttagccgat gaaccgacag gttcgctgga tcctgaaaat agagatttgg tcttgaagtt
     7981 tctcttagag atgaatcgcg aagggaaaac agttattatt gtgacccacg atgcttatgt
     8041 agcccaacaa tgtcatcgga tcattgaatt gggcgagggg aaatgagttc gttcggcttc
     8101 ttttgactgg ctgaatactc atgttttcca gagagaaata gcataaatac gcctaggaat
     8161 gacattttta tgtagtattt ctaggttttt ttgtttcaaa ttgaaaaatt tttcaattta
     8221 ggcttgacaa aggatgaata taggtgtatt atttatacaa ccaaaaagaa taaacataaa
     8281 ggaggctttg ttatgaataa gatgaagaag gtgttgatga cgatgtttgg tttagtgatg
     8341 cttcccctac tatttgcttg tagtaacaat caatcggctg gaattgaagc catcaagtcc
     8401 aaaggaaaat tggttgtagc cctcaatcca gattttgctc catttgaata ccaaaaagtg
     8461 gttgatggga aaaatcagat tgtgggttca gatatcgaat tagccaaagc tatcgcaaca
     8521 gaactaggtg tcgaattgga actatctccc atgagttttg acaatgtatt ggctagtgtt
     8581 caatcaggaa aggccgacct tgccatatca ggtgtttcta agacagatga acggagcaag
     8641 gtgtttgact tttcagtccc atactatact tccaagaaca aggttattgt aaaaaaatct
     8701 gacttggcta cttatcagtc tgtaaatgac ttggctcaga aaaaggttgg agcgcagaaa
     8761 ggttcgattc aagagacgat ggcgaaagac ttactacaaa attcttccct cgtatctctg
     8821 cctaaaaatg gaaatttgat tacagattta aaatcaggtc aattggatgc cgttatcttt
     8881 gaagaaccag ttgccaaggg atttgtggaa aataatcccg atttagcaat tgctgaaatt
     8941 gagtttgatc aagaaaagga tgattcctac gcggtcgcta tgaaaaaaga tagcaaggaa
     9001 ttgaaagaag cagtcgataa aaccattcaa aagttgaagg agtctggaga attagacaaa
     9061 ctcattgagg atgcctttaa agcatccatt gaaaaataga aagaaggaaa agaatatgag
     9121 taaagaaaaa gtaattttgg cctattctgg cggtttggat acatcagttg caattacttg
     9181 gttgaagaaa gattacgatg tcgttgcagt ttgtatgaat gtaggtgaag gcaaagattt
     9241 ggatttcatc catgataagg ctctcaaagt tggagcggtt gaatcctacg tcattgatgt
     9301 acaggacgaa tttgctacag attatgtatt ggtagccctt caagctcatg cctattatga
     9361 gcagaaatat cctttggtgt ccgccttgag ccgccctctt atttctaaaa aattggttga
     9421 aatagcgcat cagacaggag cgactacaat tgctcatggt tgtacaggta aggggaatga
     9481 ccaagtcgaa taccagattg cagtcgctaa gaaagcgaat gaagctaaaa agtgatggag
     9541 tgatgggttc agaataagag gttggtagat tatcagcctc tttcttgtcg ttgaaaaagt
     9601 gagatatatt gacttttgaa aaaaatgtca taattgagtt gggataaacg taaaagtcat
     9661 actttctcgt taaatcgttc ggcgattcat ggtgacatgg acacatagaa gtaaacacac
     9721 tatgtggctt ggcagagcta aaaacttctg gtttaacaat ggctgatatt aagaactaac
     9781 gaacaaataa tatgtaaaga gtcgaggaaa tccctcgact ttttttataa tttctttggt
     9841 tgataatggg tctctacttc ttttttgaga tgagacagca tagaagtttc ctcggtcagc
     9901 tctaggagtg agaaaccttt ttggataagt ttagcattat ccctacaaat cccaatacga
     9961 acatagcaga tagcaagcct atcgtagctt ttcttattct tggcgtcctc cagtaaagtg
    10021 tagcagattt gcttacacat gtttttatct tgattgtata gatagagtgt ggaaaggttg
    10081 agcaggattg ctattcgcaa gtcatataaa cgttgatagt ttttatagac ttctaagcgt
    10141 tgcaagattt tctcggatta ttgacaaata aacggacatt ctgatagacg attgccctag
    10201 cggcttagtg taaactttac aaatttggct tgtaattttt ttataaaatt cacttcagat
    10261 acttgacagt tattatattt tcgcttat
//
