LOCUS       NZ_CP102668            20606 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Sphingobium sp. JS3065 plasmid pNIN3, complete sequence.
ACCESSION   NZ_CP102668
VERSION     NZ_CP102668.1
KEYWORDS    RefSeq.
SOURCE      Sphingobium sp. JS3065
  ORGANISM  Sphingobium sp. JS3065
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Sphingobium.
REFERENCE   1  (bases 1 to 148600)
  AUTHORS   Li,T., Xu,J., Xu,Z.-J., Xu,Y., Spain,J.C. and Zhou,N.-Y.
  TITLE     Molecular mechanism and evolution of 1-nitronaphthalene catabolism
            in Sphingobium sp. strain JS3065
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 148600)
  AUTHORS   Li,T., Xu,J., Xu,Z.-J., Xu,Y., Spain,J.C. and Zhou,N.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2022) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai,
            Shanghai 200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. v0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 05:42:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,942
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,751
            CDSs (with protein)               :: 4,751
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 40 of 122
            Pseudo Genes (incomplete)         :: 96 of 122
            Pseudo Genes (internal stop)      :: 12 of 122
            Pseudo Genes (multiple problems)  :: 23 of 122
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:10:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 48574
            Orig. end                         :: 69180
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102668.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20606
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="1"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20606
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20606
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10606](+)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10606
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     gene            complement(646..1704)
                     /locus_tag="NUH86_RS24250"
                     /old_locus_tag="NUH86_24255"
     CDS             complement(646..1704)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1242: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763243.1"
                     /locus_tag="NUH86_RS24250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24255"
                     /product="IS110 family transposase"
                     /protein_id="WP_267253287.1"
                     /transl_table=11
                     /translation="MSYDYHIGVDYHKAYTHLVVQDVGGKVLRSGKVKNDPRSLAGFLA
                     PYLENSHAVMESSRNWTVMYDWLDELCDDVVLAHPLKVKAIADAKIKTDKIDATIRAHL
                     LRADLIPESYVPCEVARELRQALRERMFFVRLRTMVKNRVVTIVDRYPEQTRALREMSD
                     LFGKIGRGQLELLSVSTIDREHIDRALAFIDDINQRIRESEKTIRRFSKDNADVKRLKT
                     MPGVGEFFARLIAAEIDDVTRFRSAKKLAAYAGLVPSTYASGGKCWNGRIIKAGNKWLR
                     WAFVEASIPAMRAEPALKFEYERLKATKGANRAKVVMARKLLTSAYQMLRDQRDYEHRD
                     ALERPSDPSRLS"
     misc_feature    1851..1864
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.81"
                     /tool="antismash"
     gene            complement(2273..2407)
                     /locus_tag="NUH86_RS24255"
                     /old_locus_tag="NUH86_24260"
     CDS             complement(2273..2407)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NUH86_RS24255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NUH86_24260"
                     /product="hypothetical protein"
                     /protein_id="WP_267253288.1"
                     /transl_table=11
                     /translation="MADAVRIGDDVGYSLCLQAKMASRDHVLAITLDFDDPPLVDGRD"
     gene            2441..4381
                     /locus_tag="NUH86_RS24260"
                     /old_locus_tag="NUH86_24265"
     CDS             2441..4381
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1134: flavodoxin"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /locus_tag="NUH86_RS24260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24265"
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_267253289.1"
                     /transl_table=11
                     /translation="MEVGWEDALSDITARLIRVRDEHGTAAFATYKGNPTGYGFAGAMW
                     MGGFHAALGVKWRYALYADDIAAKFVASGVLFGSEAVAVKPDLWRTHFALILGANPYVS
                     HGSAFSEPRVRDALKSIVERGGRVVVVDPRRTETARAYEHVAVHPGSDAFLLIAMIRVI
                     LDEQLANRAFLDRYGKNVDRLAELLMDFSLEHCAERCRLEPDVIRALARDFARSPSAVV
                     YGRTGTCLQRFATLVNILQDTVNALTGNIEREGGNVFGWSPIAFDEQLEHAGLARSGGN
                     PTRVYKHPDVLGAHPSTSLVPDITTPGEERVRALMTFAGNPVMASAGGGDRLRDALEQL
                     DLHFSLDLYVNETNRHAHYILPVTAFYEREDLPMGILPWMLRPALWYTPPVVPPPPGVR
                     EEWRVLQDIARRMGLGGAYASKWHRRLARLGIQLTPSHLYDFMLRKSQSGDLFGLRPGG
                     LNLRKIKRNKDGLLLKSELPILPLEQRLRTADCRIDLASDTVAAELVRLKAHRDPGDLP
                     FRLIGMREVKSQNSWLHNLERTMPPARTQYALINPEDAAEKSIGDGQQVEVRSKSGAIT
                     LPAKLTADVGRGTIVIPHGWGHQGGWKRANAAGGANVNILASGLREDVEPRAGMSVLTA
                     IPIDIAAANPA"
     misc_feature    complement(4819..4833)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     gene            4854..>5543
                     /locus_tag="NUH86_RS24265"
                     /old_locus_tag="NUH86_24270"
                     /pseudo=""
     CDS             4854..>5543
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1242: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612246.1"
                     /locus_tag="NUH86_RS24265"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24270"
                     /product="IS110 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEHSTEVFVGMDVAKTRNAIAVADGERGGEVRFLGEVDASLEAMR
                     RVVQRIAAEHERIHFCYEAGPTGYGLHRLITSMGYPCDVVAPSLIPRRPGDRVKTNRRD
                     AVGLAKLLRAGELTPVWVPDEGHEAMRDLVRARAAAVETQRVHRQQVSAFMLKHGRIFP
                     RKTAWGARHLRWLQEQHFDHPAHQIALQELVDAVRTDRERIVRIEAAIEEFLPSWSLAP
                     VVRALQAL"
     gene            complement(5565..6398)
                     /locus_tag="NUH86_RS24270"
                     /old_locus_tag="NUH86_24275"
     CDS             complement(5565..6398)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072662.1"
                     /locus_tag="NUH86_RS24270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24275"
                     /product="IS5 family transposase"
                     /protein_id="WP_267249579.1"
                     /transl_table=11
                     /translation="MAWTGIARREHSREGLRYPTDMTDREWALVSPFIPPAKSGGRRRT
                     TDMRELLNALLYLAGGGCAWRMLPKCFPPVSTVRRYFYAWRNAGLFEAINTVLVMNLRE
                     IEGRDASPSAGVIDSQSVKTTESGGPTGYDAGKKIKGRKRHILTDTCGFLIFILVHAAD
                     IQDRDGAVDVLKAVRQRFPWLRHVFADGGYAGDKLRDALASMGKWTVEIIKRSDAAKGF
                     HVLPRRWVVERTFAWLGRCRRLAKDWEASIASSTAWTLIASIRMVTRRTARYCYA"
     gene            <6475..6897
                     /locus_tag="NUH86_RS24275"
                     /old_locus_tag="NUH86_24280"
                     /pseudo=""
     CDS             <6475..6897
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1242: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612246.1"
                     /locus_tag="NUH86_RS24275"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24280"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="LRGVELVVAVTFVTEVGDVRRFDSPRQLMGYLGLVPSERSTGDAI
                     RRGGITKAGNGRVRHMLVESAWTYRHPPRVGKNKLYRLEQTTPKVREIAWKAQSRLTAR
                     YRALNAKGKKTTVVCAAIARELAGFMWAIAREVQPA"
     gene            complement(7173..7361)
                     /locus_tag="NUH86_RS24280"
                     /old_locus_tag="NUH86_24285"
     CDS             complement(7173..7361)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /locus_tag="NUH86_RS24280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24285"
                     /product="transposase domain-containing protein"
                     /protein_id="WP_267253290.1"
                     /transl_table=11
                     /translation="MLPPLRGPVSEHRRHHAAGRRRAPPKTGGINPHDWLNQTLTALAN
                     GHPANRVHELMPWINVG"
     gene            7292..7576
                     /locus_tag="NUH86_RS24285"
                     /old_locus_tag="NUH86_24290"
     CDS             7292..7576
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037206.4"
                     /locus_tag="NUH86_RS24285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24290"
                     /product="S-4TM family putative pore-forming effector"
                     /protein_id="WP_267253291.1"
                     /transl_table=11
                     /translation="MRDAFRQRDAADAQKLARAEAEALWELVLAGGCDDSECERRSREF
                     QNSIFQRRTSNPLLLPFVYRWLRSGMEIDMNLGAADFLRQAGIAEVNQS"
     gene            complement(7619..8185)
                     /locus_tag="NUH86_RS24290"
                     /old_locus_tag="NUH86_24295"
     CDS             complement(7619..8185)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823967.1"
                     /locus_tag="NUH86_RS24290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24295"
                     /product="hypothetical protein"
                     /protein_id="WP_267253292.1"
                     /transl_table=11
                     /translation="MFQSSAPLEPSGNCDIAALLERLAQASSRPRYAFMLLNLIAQVAG
                     PDGSAGPWIKQDNELVSLRDWLCAALTPMAQRSARNVALAERVHRDLVRSDALPRDAQD
                     AQRAVAEEMRARIRTAGKSNLSRAVSELVNAGFLRRHYQGCRVDHQNRGGQRHAVYTLV
                     GDARLLGPTVHAPKALVQGEFAFTG"
     misc_feature    8314..8329
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.62"
                     /tool="antismash"
     misc_feature    complement(8314..8332)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: medium, score: 28.77"
                     /tool="antismash"
     misc_feature    complement(8315..8331)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 23.15"
                     /tool="antismash"
     gene            8407..9168
                     /locus_tag="NUH86_RS24295"
                     /old_locus_tag="NUH86_24300"
     CDS             8407..9168
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1197:
                     autoinducer-binding transcriptional regulator,"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849920.1"
                     /locus_tag="NUH86_RS24295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24300"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_267253293.1"
                     /transl_table=11
                     /translation="MANRIRDCGRLDDLTRFAEECARAGNLGDLQGTIDAAVRELGFRW
                     FTLLHNVDLRRGGGESLFLTTYPSAWLEEVLEERHYVEDPIHAACARTPSGLAWDRVGD
                     VLELSSRQRSILIRARDHDLAAGYSLPIRTPGEPEAIFTVARARDEPLDVEEILTARLL
                     GSVAYDRARELLGEEMRTFVFVPLSPRQVECIALVAQGKSDWEIAQILGLSRDTVHEYV
                     ESARRRYGVRRRTQLVLRAVRDGHLNMDALL"
     gene            9185..9928
                     /locus_tag="NUH86_RS24300"
                     /old_locus_tag="NUH86_24305"
     CDS             9185..9928
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1197:
                     autoinducer-binding transcriptional regulator,"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449333.1"
                     /locus_tag="NUH86_RS24300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24305"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_267253294.1"
                     /transl_table=11
                     /translation="MIGLGQVQDFCAVAATIKDSRALAGLMAEITREMGFRYFALVHHI
                     DRKPAVSPVHIVDYPPDWVERFQARRLYASDPIHRASHRTNVGFAWSAVQSLITLSAAD
                     RSILAEAHNAGLGDGFTVPAHIPGEVNGSCSFAMGSGDVLDQRQLPLVQLIGSFAFEAA
                     RRLSRQTQARCEGPSLTERQAECVALVARGKTDWEISQILGIGQETVIQHVKDARDRYG
                     VTKRTLLAIRALFDGQISFADVFGR"
     misc_feature    10001..10013
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.6"
                     /tool="antismash"
     gene            10001..10606
                     /locus_tag="NUH86_RS24305"
                     /old_locus_tag="NUH86_24310"
     CDS             10001..10606
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683387.1"
                     /locus_tag="NUH86_RS24305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24310"
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="WP_267253295.1"
                     /sec_met_domain="Autoind_synth (E-value: 6.1e-22, bitscore:
                     71.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHIETGAARAMETRLFRSMFEERKRIFVDLLHWDVPVLAGRYEID
                     QFDNDAAVYIVIADAEGEHLASARLLPTTGDHILGTIFPELCEQAPPRGGAILEITRFC
                     LARRLRARERLEVRNRLVSALVEYALRNRIQSYTGVAEWPWFQQILSFGWACRPLGLPG
                     STSCLVALQIDIDGRTREQLQASGIWRPVGLAEFAKAA"
     gene            10617..11561
                     /locus_tag="NUH86_RS24310"
                     /old_locus_tag="NUH86_24315"
     CDS             10617..11561
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449335.1"
                     /locus_tag="NUH86_RS24310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24315"
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="WP_267253296.1"
                     /sec_met_domain="PhyH (E-value: 6.8e-20, bitscore: 65.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNLSPYGQAVDPTVDDDLQRHGFSVLRDIISTTEIAEIDHALDP
                     RFAATPFCQGGFYGPRTKRFGGLLKRVPAVERLVRHPAIMRLVQSVLNPWCDTIQLNLT
                     QALELHPGALAQFPHRDQDMWRGPTGQIEYLVNVMWPISPFRGENGATLIWPESHNAGS
                     AKDAQPMAVEAEPGSAILFLGSTLHGAGANMTGAIRRGIIVSYCLGWLKPYENQWLTYP
                     PDAARAFDPELAALVGYQQHRPNLGNYDGQCPSILLEGNPPEYLAAIDALRPDQEGALA
                     EFLDQQRAGQLPPAGGIAEIGAGRNKREVKSNG"
     gene            11612..11953
                     /locus_tag="NUH86_RS24315"
                     /old_locus_tag="NUH86_24320"
     CDS             11612..11953
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039072.1"
                     /locus_tag="NUH86_RS24315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24320"
                     /product="hypothetical protein"
                     /protein_id="WP_267253297.1"
                     /transl_table=11
                     /translation="MQRVILSLAVLAMGTALQPTPARANDLGCQVLLFLSNPGGATQYA
                     QWVAPMTKLWQRLGTGGSFPGCSGGGVARTKVYDRDSATRRRVVMTFSDGRRETYSLAQ
                     IERLQDGVR"
     gene            11950..12618
                     /locus_tag="NUH86_RS24320"
                     /old_locus_tag="NUH86_24325"
     CDS             11950..12618
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039071.1"
                     /locus_tag="NUH86_RS24320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24325"
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_267253298.1"
                     /transl_table=11
                     /translation="MILDVASLLVLAGSCAPSVAPETLISIVHTESRFNTLAIGVNSPG
                     ARKPTPATRAQAIAAARSLIHQGYNIDLGLGQINSANLGWLGLSVEDAFEPCRNLAAAA
                     RVLTTNYQSVARFAPSSDHAIATALSLYNTGDRRRGFRNGYVARVYASASTVIPLIRGR
                     LPASVTTAPVQPADAVIAKPLPAALAARQAAGWNTAAETQQASLMVFGDGVTSQKGQHP
                     "
     gene            12615..12953
                     /locus_tag="NUH86_RS24325"
                     /old_locus_tag="NUH86_24330"
     CDS             12615..12953
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039070.1"
                     /locus_tag="NUH86_RS24325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24330"
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_125723801.1"
                     /transl_table=11
                     /translation="MTAITARLGSQPQWARLLLRIAGIVGLAVLLSLLLSDPAHAQGAD
                     GITSMAENIKTWLTGTFAKTIAVIAVVIVGFMFFTGRASLGLLVTVIVGIFIVFSAQWI
                     VDTITGGA"
     gene            12950..13300
                     /locus_tag="NUH86_RS24330"
                     /old_locus_tag="NUH86_24335"
     CDS             12950..13300
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449339.1"
                     /locus_tag="NUH86_RS24330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24335"
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_267253299.1"
                     /transl_table=11
                     /translation="MREAAPSNERLREETLFLAVTRPTMWLGVPLEASLPIAFAACLTL
                     IVSGNPLYAGAIGGACLAVARLIVRHDANAFRLLWLWTLTKARCRNRGWWGGSSYSPLP
                     VAGMKRKGFARG"
     gene            13293..15704
                     /locus_tag="NUH86_RS24335"
                     /old_locus_tag="NUH86_24340"
     CDS             13293..15704
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072616.1"
                     /locus_tag="NUH86_RS24335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24340"
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_267253300.1"
                     /transl_table=11
                     /translation="MASAAAQLPPRKTPWRVLAREAEPARYLPYARHVTPEVIALDSGD
                     LMMMFRLEGLAFETADPIHLNDWHEKLNGTWRNIADDRLAIWTHIVRSAVGDYPEGQFR
                     SKFAAELDGKYRARVTAKRMFVNEHYLTLLMRPAVGSADRTGLLLKRIAKARAAEKEVD
                     PDELARFEEKARDIEKLLRRCSPTRLALYEHNGLIFSAPLEVLEQLMMGARSRVPLVRG
                     HLGSALYGERVIFGRETVEIRAHDASRWQGILGIREYPALTRPGQMNALLGQDFAFVVS
                     QSFTFMGKARAAERLRRRQNQMASTEDAAASQALDLADAADDLQSNRFVLGEHHFSLAV
                     FAENQRRLADNLSAARAALADAGLVAAREGPALEAAFWAQLPGNFAWRARPAAITSRNF
                     AALAPFHTYPAGRAKGNHWGPAIAMMKTAAQSPYYFNFHVGDLGHTLIIGPSGAGKTVV
                     QNFLMAQLEKTGAQQIFIDKDRGAEIYVRSAGGIYLTLKNGVPTGFAPLRALDYGPRNL
                     AFLGRLIRQLVTPVGGQLGVTQERMIDEGLASLGRLAPEDRSILALRQLLGQRDPEGIG
                     ARLEKWARGGSLGWVFDNEIDALSLEAAFIGFDMTDFLDNPEVRTPLMMYMFHRIDGLL
                     DGRRLVIDIDEFWKALGDDAFRAFAQDGLKTYRKQNAFLVFGTQSPADALRSDISHSIM
                     EQVATKILLPNPYGRETDYVDGLGLTQAEFKLIRHDLNPESRRFLVKQGHDSIIVELDL
                     GGLSDELAVLSGTTETVTLLDAIRAEVGDDPENWLPLFHQQRRSPSSRKG"
     gene            15708..16421
                     /locus_tag="NUH86_RS24340"
                     /old_locus_tag="NUH86_24345"
     CDS             15708..16421
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683402.1"
                     /locus_tag="NUH86_RS24340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24345"
                     /product="type IV secretion system protein"
                     /protein_id="WP_267253301.1"
                     /transl_table=11
                     /translation="MRKGKTAAIGAIILGTAGPAAAQGIPVYDSSGYLQALATVKNTLS
                     MIEQGKEQIAEAKQLYGSFNQVTDVNGIAPSLSTDTMRHLLPREARDLGRLMSSDNARL
                     GSLGSAATRIRDANRIALPDLRPGASAYERASRDTLLRNGDLAARDAAIAESAYGVSAQ
                     RTTGLEELRTSLDTASDAKQVMDIQARVGVENVHIQNDALQLQALRMRQEADVRLRSQR
                     ESETILASKLGSLSQ"
     gene            16653..17597
                     /locus_tag="NUH86_RS24345"
                     /old_locus_tag="NUH86_24350"
     CDS             16653..17597
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683405.1"
                     /locus_tag="NUH86_RS24345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24350"
                     /product="type IV secretion system protein"
                     /protein_id="WP_267253302.1"
                     /transl_table=11
                     /translation="MATDLFSSLYTNIDGKLDLFLNERLQNVVEVVRGPLALGLVIYIA
                     LFGYMVMRGIISEPWGELFYRMVKLCLLYIAATTVAYSEWITTPLFHGMPDAISQALAG
                     KTIGTAGQAFDDYYAQSDVIIARIETEAATYSDINPYKLVLYALSLGLKALAGLSAAIG
                     FAITIFAKVALAIIIALGPIFIALALFEPTRRFFHGWLGQTINYIVLLAVIIAVTTLIT
                     DLGAAATAASEGVVDTAFGAVLFAAYIFLGTIFFFQAPAIATGIAGGAAAGVGAFAGTA
                     WGTMASPFRQRRIARNSRNLERAARRGGSIETA"
     misc_feature    complement(17627..17636)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 16.31"
                     /tool="antismash"
     gene            17932..18630
                     /locus_tag="NUH86_RS24350"
                     /old_locus_tag="NUH86_24355"
     CDS             17932..18630
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039065.1"
                     /locus_tag="NUH86_RS24350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24355"
                     /product="VirB8/TrbF family protein"
                     /protein_id="WP_267253303.1"
                     /transl_table=11
                     /translation="MRVADNGAEAVPGSGLKRYFQEARSWDQDRVRSALRSTRIAWTVA
                     ALTSVLAAASIFAVAALAPLKTVVPYVIRVNQTTGAVDVQTALTQRPMRYDEAVTKYFL
                     AQYVRTRESWIPAAAEENFRFVTILSQPAEQQRWARFFSNNNAASPQNAWGKNVVVQAR
                     VRNIAFINDRVANVRFTRQIQTETDTQSSDWIATVTFAYANAPMAEGDRYRNPLGFQVE
                     NYRSDPEVVR"
     gene            18627..19484
                     /gene="virB9"
                     /locus_tag="NUH86_RS24355"
                     /old_locus_tag="NUH86_24360"
     CDS             18627..19484
                     /codon_start=1
                     /gene="virB9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684609.1"
                     /locus_tag="NUH86_RS24355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_24360"
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_267253304.1"
                     /transl_table=11
                     /translation="MKTLVAAAIIAWASPFAAHAVEVPRGAASDPRVKFVDYAEAQVYR
                     IVGTFRTATQIVLSEDETIQHVALGDTVSWEVAVAGHILFLKPRERAGPTNLIVTTSRG
                     GTLRSYAFELTARSGPITGRNGQAYFQVRFRYPRDEAARAERLRAAQIAMQAAALEAQA
                     VRGALDHAVIEGPRNMNYKVQGSSDLQPSEVSDNGQFTVLRFPANREVPAIYLVRPDGS
                     ETLVPFDVRDEFVVVHLVARQLRLRRGGEVLCIYNQAPEPYGVDHGTNTGSPHVERTIT
                     HPQE"
     misc_feature    19902..19921
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 22.69"
                     /tool="antismash"
     misc_feature    complement(20241..20255)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.71"
                     /tool="antismash"
ORIGIN
        1 tatatcgccc gaatgcctgg cagcagatga atatgcccgt gacccccacg tccatcacgg
       61 cctgccagtc ttccaatggc atatgttccg gcgccccggg aatgctgata ccggcggacg
      121 tgaccaggcc ggtaacgggg ccgagttccg cctcgatgct ttctgccgca gcgttcaccg
      181 cgacgggatc ggtcacatcg acgacgtagg aatgtgcagt gagagaggcg cgcttcatct
      241 cttccaaagc ggatgcgcaa gcttccgctc gcttgtctag gatggcaacg gtcgcgccgg
      301 acctagccat cgccatagcc gcgccgagtc cgattcccga cgcgcccccc gtaacaacgc
      361 atacttcctt gctcatgcct ctctccaatg aaagaactca agaggcggag tgcacgtcaa
      421 aagcactttc cctcgtgggt tcgttgtttt tcctgtgtcc gccaacgcca gcacggacga
      481 gttgtaactt tcgaggacag gcgccctggc ctcgtttcgg cattgagcca ttctccaatc
      541 cgtgcccttg ccttataggc tcggtcacat tccggttatt cggctctcgg cttcccattc
      601 tcccagagag tgcggccccg cgatgcagga cccactagag gcacatcagg acagccgaga
      661 aggatcagac ggtcgctcta gcgcgtcgcg gtgctcatag tcacgctggt cgcgcagcat
      721 ctggtaggcg ctcgtcaaga gcttgcgcgc catgacgacc ttggcgcggt tcgcgccctt
      781 ggtcgccttc aggcgctcat attcgaattt gagggcaggc tcggcgcgca tggcaggaat
      841 gctggcttct acgaaagccc agcgcagcca cttattgcca gccttgatga tccgcccgtt
      901 ccaacatttg ccgccgctcg cataggtcga aggcacgagc ccggcatagg cggcgagctt
      961 cttggccgaa cggaagcgtg taacatcgtc aatctcggcg gcgatcaggc gcgcgaagaa
     1021 ctcgcccacg ccaggcatcg tcttcagacg tttgacgtcc gcattgtcct tgctgaagcg
     1081 acggatcgtc ttctcggact cgcggatccg ctggttgatg tcatcgatga aggcaagcgc
     1141 gcgatcgata tgctcgcgat cgatcgtcga caccgaaagc agttcgagct ggccccgccc
     1201 gatcttgccg aacagatccg acatctcccg cagcgctcgg gtctgctcgg gatagcggtc
     1261 gacgatcgtc accacccggt tctttaccat cgtgcgcagc cgcacgaaga acatccgctc
     1321 tcgcaacgcc tggcgaagtt cgcgcgcgac ctcacatggt acataggact ccgggatcaa
     1381 atcggcccgc agcagatgcg cccggatcgt cgcgtcgatc ttgtcggtct tgatcttggc
     1441 gtcggctatc gccttcacct tcagcgggtg cgccaggaca acatcgtcgc acaactcgtc
     1501 cagccagtcg tacatcaccg tccagttgcg gctcgattcc atcaccgcat ggctgttctc
     1561 cagatagggc gccaaaaaac cggcaagcga gcgcgggtcg ttcttcacct tgcccgatcg
     1621 cagtaccttc ccgccgacat cctgcacaac cagatgcgta taggctttgt ggtaatccac
     1681 gccgatatga taatcgtaag acatgggtgc atctccattg ctgatgggct tcagcaattt
     1741 gcttaacact cgtgcctgca cgaggggggg cacccttgcc tctatggtga tcatcaccgc
     1801 tccattcctt cactgaagct agtcgctttc ataccggctc cttgtaccaa tctaaatatt
     1861 actaagatct gcctcgtgat actatggttc gaccatcgat gtgcctagca ctcgacatat
     1921 tacctaagaa attcgcaacc tatccagctc ggacgcgatc atccgtgcgt gaacttgcat
     1981 gccaaagacc tcgccgcctt ttaatcgatc gagtaatatc gtcaagttag catagcgtcc
     2041 actgttatgc ccagccatgt gtggatgtgc ccgcagatgg attgtttatt tcattaatta
     2101 tgttgcgagg ccagatcatt gaagataagg tggtgtcatg aattggcttc catgcctgcg
     2161 agccgctgtt cgtaaagttc tggcgctcag cagacgggtt ctcggtgcag gagatctggc
     2221 cgatccccgc cgtgtgcgaa cctattgtcg tgtctgtgag ccgctatgcg ggctaatcgc
     2281 gaccgtcgac caggggagga tcgtcaaaat cgagggtaat cgcgagcacg tggtctcgcg
     2341 aggccatctt tgcctgaagg cacaggctgt agccgacgtc gtcaccgatc ctgaccgcgt
     2401 ccgccatccg ctgaagcgca tcggcggccc tggcgaattc gtggaggtcg gttgggaaga
     2461 cgcgctgtct gacatcacag cgcgcctgat acgggtccgc gacgagcacg gaacggccgc
     2521 ctttgcgacc tacaagggta atccgaccgg atacggcttt gccggcgcaa tgtggatggg
     2581 cggatttcac gcggcattgg gtgtcaaatg gcgctatgcc ttgtacgccg acgatatcgc
     2641 ggccaagttc gtcgccagcg gggttctgtt cggatcggaa gcagtggccg tcaaacccga
     2701 tttgtggcgc acgcatttcg cgttgatcct gggcgccaac ccctatgtct cgcacgggtc
     2761 tgccttcagc gagccgcgcg tccgcgacgc cctgaagagc attgtcgagc ggggcggacg
     2821 tgtcgtggtg gtcgacccga ggcgcacgga aacggcgcga gcatacgaac acgtcgcggt
     2881 ccacccgggc agcgacgcgt ttttgctaat tgcgatgatc cgcgttattc tggacgaaca
     2941 gctcgccaat cgcgcgtttc tggatcgata cggcaagaat gtcgatcggc ttgccgagtt
     3001 gctcatggat ttctctcttg agcattgtgc cgagcgatgc cggctggaac cggatgtcat
     3061 ccgcgccttg gcgcgagatt tcgcacgatc gcccagtgcg gtagtctatg gccggacggg
     3121 cacctgcctg cagcgcttcg cgaccttggt gaacatcctt caggacacgg tgaatgcgct
     3181 gaccgggaat atcgagcgcg aaggtggcaa tgtctttgga tggtcgccga ttgccttcga
     3241 cgagcagttg gaacatgcgg ggcttgcccg ttcgggcggc aatccgactc gtgtctacaa
     3301 gcatcccgat gtcttgggcg cgcatccctc gacatcgctc gtcccggata tcaccacgcc
     3361 gggcgaggaa cgcgttcgcg cgctcatgac ctttgccggc aatccggtga tggccagtgc
     3421 cgggggcggc gatcggctgc gcgacgcgct cgagcagctc gatctccatt tctcgcttga
     3481 cctttacgtc aacgaaacga accgacatgc gcattacatt ctcccggtca cggcatttta
     3541 cgaacgggaa gacctgccca tgggcatcct gccgtggatg ctgcgtcctg cattgtggta
     3601 caccccgccg gtcgtaccgc caccgcctgg cgtcagggag gagtggcgcg tcctgcagga
     3661 tattgcccgc cgcatgggac tgggcggagc atacgcatcg aaatggcatc gtcggctggc
     3721 gcgtcttggc attcaactca cgcccagtca cttgtatgac ttcatgctcc gcaaaagcca
     3781 gtcgggcgac ctgttcggcc tgcgaccagg cggtttgaac ttgcggaaaa tcaagcgcaa
     3841 caaggacggc cttctactca aatcagagct tccgatcctg ccgctggagc agcggttgcg
     3901 cacggccgat tgcaggatcg atctggccag cgacaccgtt gccgcagagc tcgtgcggct
     3961 gaaggcccat cgagatcccg gcgatttgcc cttccgcctc atcggcatgc gggaggtaaa
     4021 gtcacagaac agttggctgc ataatctcga acgaacgatg ccgcccgctc gcacgcaata
     4081 cgcgctgatc aatccggagg acgcggcgga gaaaagcatt ggcgacggcc aacaggttga
     4141 agtgcgctcc aaaagcggcg caatcacctt gcctgcgaag ctgactgcgg acgtcgggcg
     4201 gggtacgatc gtaattccgc acggctgggg tcatcaaggc ggctggaagc gagccaacgc
     4261 tgccggcggc gccaacgtca atatccttgc aagtgggctg cgggaagatg tcgagccgag
     4321 agctggcatg tcggtattga ctgcgatccc gatcgatatt gcagcggcta acccggcttg
     4381 acagaaactc tattcattac agccgcggcc gggatgtcgc cttccgagag gcgatcggcg
     4441 acttgccagt tcggcatgct gtaccacgtc agcgcgtcgc gcgattggcc ggcaatccgc
     4501 tgagtgccta gtacagcgca acgcgaacgg ccacctgatc gttgcgtagc tatatcagcg
     4561 tcaggtcgat aggcagcggc gtgcgctacg catcgatctg ctcgatcggg ggcgaatagt
     4621 aagcgcggta gggggttcgc tacatgacgc agcgcttcgg cgacgcggca cgctggtcgt
     4681 cggcgagcgt tgctgcttgt ccatgcgcga cgacggccag ctgatcctcg acttcgcgga
     4741 acccgacgta agaccgtatg tccgctcttt ggctgagggt ggctcgccct acactgtggt
     4801 ggcgagggtt gctcggagtg gccgagccga tcctcatcgc aggaggatga gccgtggagc
     4861 attctacaga agttttcgtc ggaatggatg tggcaaagac gcggaacgcg attgcggtcg
     4921 cggacggcga gcgcggaggc gaggtgcggt tcctgggaga agtcgacgcc tctctcgagg
     4981 ccatgcgacg ggtagtgcag cggatcgccg ccgagcacga acgtatccat ttctgctacg
     5041 aagccgggcc gacgggctat ggcctccacc ggttgatcac atccatgggc tatccctgcg
     5101 acgtcgtcgc accttcgctg ataccgaggc gccccggcga cagggtaaag accaataggc
     5161 gtgatgcggt cggtttggcc aagctcctgc gcgcagggga actgacgccg gtgtgggttc
     5221 ctgatgaggg ccatgaagcg atgcgcgacc tggtccgcgc tcgcgctgcg gcggtcgaga
     5281 cccagcgcgt tcaccgacaa caggtgagtg cgttcatgct gaagcacggg cgcatctttc
     5341 cgcgcaaaac ggcatggggg gcacgccacc tccgctggtt gcaagaacag cacttcgatc
     5401 atcccgctca ccaaatcgca ctgcaggaac tcgtcgatgc ggttcgcacg gacagagagc
     5461 gtatcgttcg catcgaggcc gcgatcgaag agtttctgcc cagctggtca ctggcaccgg
     5521 tcgttcgcgc gctccaggcc ttaagtgtcc gattcagaag tttatcaagc atagcaatac
     5581 cttgcggttc gtcgagtgac catgcgtatg gaggcaatga gggtccaagc agttgatgag
     5641 gcgatggatg cctcccagtc tttggcgagg cgacgacatc tgccgagcca tgcgaaagtg
     5701 cgctcgacca cccaccggcg gggtaggaca tggaagccct tggcagcgtc ggaccgcttg
     5761 atgatttcga cagtccactt tcccatcgag gccagtgcgt ctctgagttt gtcccctgca
     5821 tagccgccgt ctgcgaagac gtgtcgtagc caaggaaagc gttggcgcac tgctttgagg
     5881 acatcgacgg caccgtcccg gtcctggatg tctgcggcat ggaccaggat gaagatcaga
     5941 aagccgcaag tgtcagtgag gatatggcgc ttccttccct tgatcttctt gcctgcatca
     6001 tagcccgtgg ggccgccgga ttctgtggtc ttcacactct ggctgtcaat caccccagcg
     6061 ctgggcgagg cgtcgcgtcc ctcgatctca cgcaggttca tcaccagaac cgtgttgatc
     6121 gcctcgaaca atccagcatt gcgccaggcg tagaaatagc gccgcaccgt cgagaccggc
     6181 gggaagcatt tgggcagcat gcgccacgca cacccgcccc cggcaagata gagcagcgca
     6241 ttcaacaatt cccgcatgtc ggtcgttcgg cggcgaccgc cggatttggc tggaggaata
     6301 aaaggcgaaa ccaacgccca ttcccgatcc gtcatatcgg ttggatatct caatccttcc
     6361 cggctatgct cccgccgggc aataccagtc catgccatgg ggcactccat ctcttcgcaa
     6421 ggacggcgtg aatcacaaca ggctggtatt gctcaacaac tttcggatct ggctctaaga
     6481 ggcgtggagc ttgtcgtcgc ggtcactttc gtcaccgagg ttggcgatgt gcgccggttt
     6541 gacagcccgc gccagttgat gggatatttg ggcttggtgc caagcgagcg ttcgactggc
     6601 gacgcgatcc gacgtggcgg gatcaccaag gccggcaacg gaagagtccg acacatgctg
     6661 gtggagagcg cctggactta ccgtcacccg ccccgggtgg gtaagaacaa actgtaccgc
     6721 ctggagcaga ccacacctaa ggtgcgggag atcgcctgga aggcgcagag ccgtttgacg
     6781 gctcgctatc gagcgctcaa cgcgaagggc aagaagacca cggtagtttg cgcggcgatc
     6841 gcacgcgagc tcgccggctt catgtgggcg atcgcccggg aggtccaacc cgcctgacga
     6901 tcacaatagc tcccgcgcac aggcgggggc gggaccacgg caggggaata tccgtcacac
     6961 gctttgtggc cggcatcgcc gacgcccgct gtaagatagg atcagccccg gacgcacaat
     7021 cgggaatgcg gtagccaacc cgcgtatcag agcttgatca ccgacgtctc tcaggtcccg
     7081 tctccaccta tgcgcggtca ccacggcaca gcttcgccca gcggtggctt tgcgcgcccg
     7141 caacgttgac agcggacatc agagcggtgt tctcagccca cgttgatcca gggcatcagt
     7201 tcatgaactc gattggccgg atggccgttg gcgagcgcgg tcagcgtctg gttcagccag
     7261 tcatgcggat tgatgccacc cgtcttgggc ggtgcgcgac gccttccggc agcgtgatgc
     7321 cgccgatgct cagaaactgg cccgcgcaga ggcggaagca ttgtgggaac tggtgctcgc
     7381 gggaggttgt gacgacagcg aatgcgaaag acggtcgcgc gaatttcaga acagcatatt
     7441 ccagcgcaga accagcaatc cgctgctcct gcctttcgtc tatcgttggc tccgatccgg
     7501 aatggagatc gacatgaacc taggggccgc tgattttctg cgtcaggctg ggatagccga
     7561 agtcaatcaa tcctgacggc aatcttgttc aaatgctgca ggctggatgc aagtctgctt
     7621 agcccgtgaa cgcgaattca ccctggacca acgctttcgg cgcgtgaacc gtcgggccga
     7681 gaagacgcgc atccccaacc aatgtataga cggcgtggcg ctgcccgccg cggttctgat
     7741 ggtcgacgcg acagccctga taatggcggc gcaggaatcc tgcatttacg agctcggaaa
     7801 ccgcacggct gagattgctt ttgccggcgg tccgaatgcg cgcacgcatt tcctcagcga
     7861 ccgcacgctg tgcatcctgc gcatcgcgcg gaagcgcgtc gcttctgacg aggtctctat
     7921 ggacccgctc ggccaaggcg acgttacgcg cgctgcgctg ggccatcggc gtgagcgcag
     7981 cgcaaagcca gtcgcgcagc gaaacaagct cgttgtcctg cttaatccag ggaccggcgc
     8041 tgccgtcggg gccggcgacc tgggcaatca ggttgagcag catgaaggcg tagcgggggc
     8101 ggcttgaggc ctgcgccaat ctctcgagta aggctgcgat atcgcagttg ccggaaggtt
     8161 cgagaggagc cgaggattga aacataacgc gactgaatca agcacaaaaa aggatcaatg
     8221 tgaatccgaa atccgcaccg ttgggcatca tttgtcgcct atgacacttt accagcgggc
     8281 aaagtgtcat aggcgattca ttcctcgaag actgttgcga atcgttctca actcgccgtg
     8341 ataatcaccg tgctgatcgc ttatcactgg acggggagtt gtcctggcga cgagtttcgg
     8401 ccgtctatgg cgaacaggat aagagattgc ggccgcctgg atgacctcac gcgtttcgca
     8461 gaggaatgtg cgcgtgctgg aaacctcggc gatctgcaag gcaccatcga tgcggccgtg
     8521 cgcgaacttg gcttccgctg gttcacgctg ctccacaatg tcgatcttcg gcgcggaggc
     8581 ggggaaagcc tgtttctcac cacctatccg tcggcctggc tcgaggaggt gctcgaggag
     8641 cgccactatg tcgaagaccc catccatgcg gcctgcgccc gcacgccatc ggggctcgcc
     8701 tgggatcggg tgggcgacgt gcttgagctt tcttcgcgcc agcggagcat cctgatacgc
     8761 gccagggatc atgaccttgc agcgggctat agcttgccga tcaggacgcc gggtgagcct
     8821 gaggcgattt tcacggtcgc ccgagcgcgt gatgaaccgc tggatgtgga ggaaattctc
     8881 accgcccgct tgctgggttc ggtcgcctat gaccgggcgc gcgaactgct cggcgaagag
     8941 atgaggacct tcgtgttcgt tcccctcagt ccgcgccaag tcgaatgcat cgctcttgtg
     9001 gcccagggga agagcgattg ggagattgcc cagatactcg ggcttagccg cgacaccgtg
     9061 cacgaatatg ttgaatctgc gcgtcggcgc tacggcgttc ggcgccgaac gcagctagtg
     9121 ctgcgcgcgg ttcgcgacgg tcatctcaac atggacgcgc tcctctgatc tcgcttgggc
     9181 cgtgatgatc gggcttggcc aagttcagga cttctgcgcg gtcgccgcga caatcaagga
     9241 cagtcgagcg ctcgccggcc tgatggccga gatcacgcgt gagatgggtt ttcggtactt
     9301 tgccctggtc caccatatcg accgcaagcc ggcggtgagc cctgtccata tcgtcgatta
     9361 tccaccggac tgggtggagc gtttccaggc gcgtcgcctc tatgccagtg acccgatcca
     9421 ccgtgccagc caccgcacca atgtcggctt tgcctggtcg gcggttcaat cactcatcac
     9481 gctcagtgct gctgatcggt cgatcctggc ggaagcgcac aacgccgggc tgggcgatgg
     9541 tttcacggtt cctgcccaca ttcctggtga ggtaaatggg tcctgctcat tcgccatggg
     9601 gtcaggcgac gtgctcgatc aacgacagct cccgctcgtc cagctgattg gcagcttcgc
     9661 attcgaagct gcgcgcagat tgtcccggca aacccaagct cgatgcgaag gccccagcct
     9721 gactgagcgg caggctgaat gtgtggcgct ggtcgcgcgc gggaagaccg actgggagat
     9781 cagccagatc ctcggcattg ggcaggaaac ggtgatccag catgtcaagg atgcgcgtga
     9841 ccgctacggt gtcacgaagc gcacgcttct ggcgatccgc gccttgttcg atggacagat
     9901 cagcttcgcg gatgttttcg gccgatgatc tccccaggac tggggataac cgaaaggacg
     9961 cgggcctgat cgactgaccg cctcatcaaa ggaggctgtc atgcatattg aaaccggcgc
    10021 tgcccgcgcc atggaaaccc gcctgttccg ttcgatgttc gaggaacgca aacggatatt
    10081 cgtcgatctt ctgcactggg acgtgccggt cctcgccggc cgctatgaaa tcgaccagtt
    10141 cgacaatgac gctgccgtct acatcgtgat agccgacgcc gagggcgagc atcttgcctc
    10201 ggcgcggctg cttcctacga ccggtgacca tattcttggc accattttcc ccgagctatg
    10261 tgaacaagcg cctccccggg gtggtgcgat cctcgagatc acgcgctttt gtcttgcacg
    10321 acgattgcgc gcacgtgagc gcctggaggt gcgcaaccgg ttggtgtccg cgctcgtcga
    10381 atatgcgctg cgaaatcgaa tccaaagcta caccggcgtc gctgagtggc cctggttcca
    10441 gcagatcctg agctttggct gggcctgccg ccccctcggc ctgcccggtt cgacaagctg
    10501 ccttgtcgcc ttgcagatcg acatcgatgg ccgcacgcgt gagcagctgc aggccagtgg
    10561 catctggcgc ccggtcggtc tcgccgaatt tgccaaggct gcttgaggag ggcgccatga
    10621 cgaatctctc cccctatggc caagctgttg accctacggt cgatgacgat ctgcagcggc
    10681 acggcttcag cgtgttgcgg gacatcatat ccacgacaga gatcgcggag atcgatcacg
    10741 ccctcgatcc gcgctttgcc gccacaccct tttgtcaggg cggtttctac ggtccgcgca
    10801 ccaagcgctt cggcggcctt ctgaaacgcg tacccgctgt cgagaggctg gtgcgtcatc
    10861 ccgcgatcat gcgcctggtg cagtcggtcc tcaacccatg gtgcgatacc atccagctca
    10921 acctgacgca ggcgctcgaa ctgcatcccg gcgcgctggc gcagttccct cacagggatc
    10981 aggatatgtg gcgcggcccg acaggccaga tcgagtatct cgtcaacgtg atgtggccga
    11041 tttctccgtt caggggtgaa aacggcgcca cattgatctg gcccgagagc cataatgccg
    11101 gttctgcgaa ggacgcgcag cccatggcgg ttgaggcgga gccgggctcg gcgatcctct
    11161 ttctcggttc cacgctgcat ggcgccggcg cgaacatgac cggcgcgatc cggcgtggca
    11221 ttatcgtcag ctattgcctg ggctggctga agccctacga aaatcaatgg ctgacctatc
    11281 cgcccgatgc ggcccgtgcg ttcgatccag agcttgccgc gctcgtcggt taccagcagc
    11341 accgccccaa tctcggcaat tatgatggcc aatgcccgtc catcctgctc gagggtaatc
    11401 cacccgaata tttggcggcg atcgacgcgt tgcgccccga tcaggaaggt gcgctggctg
    11461 aattcctaga ccagcaaaga gcaggccaac tccccccggc tggggggatt gccgaaatcg
    11521 gagccgggcg gaacaaaaga gaggtgaaat ccaacggata gccgacgcca attcccaacg
    11581 cgggaggtgt ctctcgcggg ggaatgaccc gatgcaacgt gtgattctca gcctggcggt
    11641 gctcgccatg ggcaccgctc ttcaaccgac tcccgcgcga gcgaatgact tgggctgcca
    11701 ggttctccta ttcctttcca atcccggtgg cgcgacgcaa tatgcccagt gggtcgcgcc
    11761 catgaccaag ctgtggcagc gcttgggcac tggcggctct tttcccggct gtagcggcgg
    11821 tggtgtcgct cgtaccaagg tctacgatcg cgattccgca acgcggcgcc gggttgtcat
    11881 gaccttcagc gatggacgcc gcgaaaccta ctcgctggcc caaatcgagc ggctgcagga
    11941 cggcgttcga tgatcctcga cgtcgccagt cttctcgtcc ttgcgggctc atgcgcgcca
    12001 agcgtggcgc ctgagacgct aatctcgatc gttcacacgg aaagccggtt taacacactg
    12061 gcgatcgggg tgaacagtcc gggtgcgcgt aagccgacgc cggcgacgag ggcacaggcg
    12121 attgctgcgg cgcgtagcct catccaccaa ggctacaaca tcgacctggg cttggggcag
    12181 atcaacagcg ccaacctcgg atggctgggt ctgtcggtgg aggatgcctt tgagccgtgc
    12241 cgcaacctcg cagccgcagc gcgggtgttg acgaccaact atcagtcggt ggcgcgtttc
    12301 gcgccgagtt cggaccatgc gatcgcaaca gcgctctcgc tctacaatac cggcgatcgg
    12361 cgccgcggat ttcgcaacgg ctatgtcgct cgggtttatg ccagcgcgtc aaccgtcatt
    12421 cccctgatcc gcggcaggct cccagcgtct gtgacgaccg cgcctgtcca gcctgccgat
    12481 gcggtgatcg ccaaaccgct acccgctgcg ctcgcggcga ggcaggccgc cgggtggaac
    12541 accgccgcag aaacccagca agcctcgctc atggtcttcg gggacggggt aacttctcag
    12601 aaaggacagc atccatgaca gcgatcaccg cccgcctggg ttcccagccc caatgggcgc
    12661 gtcttttgct gcgcatcgcc ggcatcgttg gcctggcggt acttctctcc ttgctgctga
    12721 gtgatcctgc ccatgcgcaa ggcgcggacg gcatcacctc catggcggag aacatcaaga
    12781 cttggctgac gggcaccttt gccaagacga ttgcggtgat cgccgtcgtc atcgtcggtt
    12841 tcatgttctt caccggacgg gcgagtcttg gcctattggt gacggtcatc gtcggcattt
    12901 tcatcgtctt cagcgcgcag tggatcgtcg ataccatcac gggtggcgcg tgagggaggc
    12961 cgcgccctcg aacgagcggc tgcgcgagga aacgcttttc cttgcggtga cccggccgac
    13021 gatgtggctg ggcgtgccgc tcgaagcgtc cttgccgatt gcgtttgctg cctgtctcac
    13081 cctgatcgtc agcggcaatc cgctttacgc gggggcgatc ggcggcgcgt gcctggcggt
    13141 ggcgcgcctc atcgtgcgcc acgacgccaa tgcctttcgt ctgctatggt tatggacttt
    13201 gaccaaggcg cgctgccgga accgcggctg gtggggcggc agttcctatt caccgctgcc
    13261 ggtcgcgggg atgaagcgca agggcttcgc gcgtggctag cgccgccgcc cagctgccgc
    13321 cgcgcaagac gccctggcgg gttctggccc gagaggcgga gcccgcgcgt tatctcccct
    13381 atgcccgcca tgtgacgccc gaagtgatcg cgctcgatag cggcgatctc atgatgatgt
    13441 tccgtctcga agggctggct ttcgagacgg ctgatccgat ccacctcaac gattggcacg
    13501 agaagctcaa cggcacctgg cgcaacatcg ccgacgatcg gcttgcgatc tggacccaca
    13561 tcgtccgctc agccgtaggg gactatcccg aagggcagtt tcgttcaaag ttcgccgccg
    13621 agctcgacgg taaatatcgg gcgcgcgtca ccgccaagcg gatgttcgtc aatgagcatt
    13681 atctgacgct tctcatgcgt ccggccgtcg gatcggccga tcgaaccggg ctgcttctca
    13741 agcggatcgc gaaagccagg gcagcggaga aggaagtcga tcccgacgaa ctggcgcgct
    13801 tcgaggagaa ggcgcgcgac atcgagaagc tgctccgccg ctgcagcccg acgcgcctcg
    13861 ccctctacga gcataatggc ctgatcttct ccgccccgct tgaggtgctc gagcaattga
    13921 tgatgggcgc gcgatccagg gttccgctcg tccgcggtca tctgggatcg gcgctttatg
    13981 gcgagcgcgt gatcttcgga cgcgagacgg tcgagatccg tgcccatgac gcgagccgct
    14041 ggcagggcat cttggggatc agagagtatc ccgcgctgac gcgcccgggc cagatgaatg
    14101 ccttgctcgg ccaggatttc gctttcgtcg tctcgcaatc cttcaccttc atgggcaagg
    14161 cgcgggcagc cgaacgtctg cgccgccggc agaaccagat ggcctcgacc gaggatgcgg
    14221 ccgcgagcca ggcgctcgat cttgctgatg ccgccgacga tctgcaaagc aaccgcttcg
    14281 ttcttggtga gcatcatttc tcactggccg tattcgccga gaaccagagg cggcttgccg
    14341 ataatctctc ggccgcgcgc gcggcgctgg ccgatgccgg cctggtggcg gcgcgcgaag
    14401 gaccagcgct cgaagcggcg ttctgggcgc aactgcccgg aaatttcgca tggcgggcgc
    14461 ggccggcggc gatcacctcg cgcaattttg ccgccttggc gccgttccat acctatcccg
    14521 cgggccgtgc caaggggaac cattgggggc ctgcgatcgc catgatgaag acggccgcgc
    14581 aatcgcccta ttatttcaac ttccatgtcg gcgatttggg ccacacactg atcatcggtc
    14641 cttctggcgc gggcaagacc gtcgtccaga attttctgat ggcgcagctc gagaagacgg
    14701 gcgcgcagca gatcttcatc gacaaggacc gcggcgcgga gatctatgtc cgatccgcgg
    14761 gcggaatcta tctaacgctg aagaatggcg tgcctacagg tttcgcgcct ctgcgggcac
    14821 tcgactatgg tccgcgcaat cttgccttcc tggggcgcct gatccgccag ctcgtgacgc
    14881 cggtcggagg tcagctaggg gtcacgcagg aacgcatgat cgacgagggg ctggcctcgc
    14941 tcgggcgcct cgcgccggag gatcgctcca ttctcgcgct tcgccagctg ctcggccagc
    15001 gtgaccctga aggcatcgga gctcggctgg agaaatgggc gcggggcggt tcgctcggct
    15061 gggtgttcga caatgagatc gatgccctat cgctcgaggc ggcgtttatc ggcttcgaca
    15121 tgaccgattt tctcgacaac cctgaagtcc gcacgccgct catgatgtac atgttccacc
    15181 ggatcgacgg tctcctcgac ggtcggcggc ttgtgatcga catcgacgaa ttctggaagg
    15241 ccttgggtga cgacgccttc cgcgcctttg cgcaggatgg cctcaagacc taccgcaagc
    15301 agaatgcctt tcttgtcttc ggcacgcaaa gccctgcgga tgcgctgcgt tcggacattt
    15361 cgcacagcat catggagcag gttgcgacca agatactgct tcccaacccc tacggccgcg
    15421 aaacggacta tgtcgatgga ctgggtctga cccaggcgga attcaagctc atccgccacg
    15481 atctgaatcc ggaatcccgc cggttcttgg tcaagcaagg ccatgactca atcatcgtcg
    15541 agctcgatct tggcggtctc tccgacgagc tggcagttct ctcgggcact actgaaacgg
    15601 tgacgctgct cgacgctatc cgcgccgagg tcggcgacga tcccgaaaac tggctgcccc
    15661 tctttcacca gcagcgtcgg tctccatcga gcaggaaagg atgagctatg cgtaaaggca
    15721 aaaccgcagc gatcggtgcg attatcctgg gaacagcagg tccggccgcc gcccaaggca
    15781 taccggtcta tgacagctct ggctatttgc aggcgctcgc cacggtcaag aacaccctgt
    15841 caatgatcga gcagggcaag gagcagatcg ccgaggccaa gcagctctac ggcagcttca
    15901 atcaggtcac cgacgtcaac ggcattgccc cgtcgctttc caccgacacg atgcgccatc
    15961 tgctgccgcg cgaggcccgc gacctcggca ggttgatgtc gtcggacaat gccaggctgg
    16021 gatcgcttgg cagtgccgcg acccggattc gcgatgccaa ccgcattgcg ctgccggacc
    16081 tgcggccagg cgcctcagcc tatgagcgcg cgtcgcgcga cactctgttg cgaaatggcg
    16141 atctagccgc ccgcgatgcc gcgatcgcgg agtcagccta tggcgtctcg gcccaacgca
    16201 caacgggctt ggaagaactg cgcacctcac tcgataccgc ttcggacgcg aaacaggtca
    16261 tggacatcca ggcaagggta ggggtcgaaa acgtccacat ccaaaatgat gccctgcaat
    16321 tgcaggcgct gcggatgcgg caggaagccg acgttcggct acggtcgcag cgcgagagcg
    16381 agacaatatt ggcttcaaag ctcgggagcc tatcacaatg acgggcaagg ttttcttgct
    16441 ggcggcattg gcgacagctg tcttaaccgg gtgtgagcga gcgccgcgcg ggaaggacta
    16501 tctgaattcc catcctgagg aactgagcga gcttactaag gcttgcgccg atggctcgca
    16561 ccagaaccag caagagtgct cgaacgccga aagccttcga acactcaaca ataaaatgca
    16621 gtcgctttca cggtagcgcg ttggaattcg gcatggctac ggacttgttc agctcacttt
    16681 acacgaacat tgatggaaag ctggaccttt tcctaaatga gcgtcttcaa aatgtggtcg
    16741 aggttgtgcg cgggccgctg gcactcggcc ttgtaatcta tatcgccctt tttggttaca
    16801 tggtgatgcg tgggatcatc agcgagcctt ggggcgagct gttctaccgg atggtcaagc
    16861 tttgcctgct gtatattgcc gcaacgacgg tcgcctattc tgagtggatc accacgccgc
    16921 tttttcatgg gatgccggac gcgatctcgc aggctctcgc ggggaagacg atcgggacag
    16981 ctggccaagc ttttgacgat tactacgccc aaagcgatgt cattatagcg cgcattgaga
    17041 ccgaggcggc gacttacagc gatatcaatc cttataaatt ggtactttat gcgttgagct
    17101 tggggcttaa ggcgctcgca ggtctctcgg cggctatcgg cttcgcaatt acgatatttg
    17161 ccaaagttgc actcgcgatc attatagccc tcggccccat tttcatcgcc ctcgcattgt
    17221 tcgaaccgac aaggcgtttc tttcatggat ggcttggcca aaccataaat tacattgtct
    17281 tgcttgctgt aatcattgcg gtcacgaccc tgattactga tctcggagcc gctgcaacgg
    17341 cagcatctga aggagtcgtg gatacggcat tcggtgccgt tctgtttgct gcatacatat
    17401 ttcttggcac aatcttcttc ttccaggctc ctgcgattgc caccggcatt gcgggcgggg
    17461 cagccgccgg ggtgggtgcc ttcgctggga ccgcatgggg gacaatggca tctcccttcc
    17521 gacaacgccg cattgcgcgc aacagccgca atctggagcg cgccgctcgt cggggcggct
    17581 ccatcgaaac ggcatagcga gacatcacca tgaccttgcc ctgtcctcaa aaatcgacgt
    17641 tcaccatccg ccaggttact ttctgcctga tcggcagtct tccattgctt ggcctcgcgg
    17701 catgcgcttc cggacccaag aagctggccg tttgtaacgg gcaccatctg cgtgacgtga
    17761 atatctatgg gagcgttctg cccggatcgc cggtgccggc gactacagcg ccgcgccctg
    17821 cgccgcccat tcctcccttg tctcatccgc aggtgggaga tccaccgcct ccgcccgcag
    17881 tgccagcgcc tccggacgac aaggtttcct ttgcgccaag ggacattcgt tatgcgagtt
    17941 gctgacaatg gcgcggaggc ggtgcctggt tccgggctca aacgctattt ccaggaagcg
    18001 cgaagctggg accaggacag ggttcgatcg gcattgcgat cgacacgtat tgcttggacc
    18061 gtggcggcac taacctcggt gcttgcggcc gcatccattt ttgctgtcgc cgcgctggcc
    18121 ccgctcaaga ccgtcgtgcc ctatgtcatt cgggtcaatc agacgacggg cgcggtggat
    18181 gtccagacag cgctgaccca gcggccgatg cgctatgatg aagcggtcac taaatacttt
    18241 ctcgcgcaat atgtacgcac acgcgaaagc tggataccgg cagctgccga ggagaatttc
    18301 cgcttcgtga cgatcctgtc gcagcctgcg gagcagcagc gctgggcgcg cttcttcagc
    18361 aacaacaatg ccgcaagtcc ccagaacgcc tgggggaaga acgtcgttgt gcaagcacga
    18421 gtgcgcaaca tcgccttcat caatgatcgg gtcgctaacg tgcgttttac gcggcagatc
    18481 cagacagaga cggatacgca gagcagcgat tggatcgcca cggtcacatt cgcttatgcc
    18541 aatgccccaa tggccgaagg cgaccgttat cggaacccgc tcggcttcca ggtcgagaat
    18601 taccgctccg atccggaggt ggtccgatga aaacattggt cgctgcggct atcatcgcgt
    18661 gggcctcgcc gtttgctgcc catgcggtcg aggtcccgcg tggcgctgcg tctgacccgc
    18721 gcgtcaagtt cgtcgattat gcggaggcgc aggtctatcg catcgtcggg accttccgga
    18781 cagcaaccca gatagtcctg agcgaggacg agacgatcca gcacgtcgcg ctgggcgaca
    18841 ccgtctcctg ggaggtggcc gtggctggcc acatcctgtt cctcaagccg cgcgaacgcg
    18901 ctggaccgac caaccttatc gtcaccacgt ctcgcggcgg aaccttaaga agctatgcct
    18961 ttgaactcac tgctcgcagc ggtccgatca ccggccgcaa cggccaggcc tatttccagg
    19021 tgcgcttccg ctatccgcgg gacgaggcgg cgcgggccga acgactgaga gcagcacaga
    19081 tcgcgatgca agcggcggcg ctggaggccc aggctgttcg cggtgccctc gatcatgcgg
    19141 tgatcgaggg gccgcgcaac atgaattaca aggtccaggg ctcgagcgat ttgcagccgt
    19201 cagaggtctc tgacaatggc caattcacgg tgttgcggtt tccggcgaac cgggaagttc
    19261 ctgccattta tctggtgagg ccagacgggt ccgaaacgct ggtccccttc gacgttcgcg
    19321 acgaattcgt tgtcgttcat ttagtggcgc gtcaactgcg cctgcgccgg ggcggcgagg
    19381 tgctgtgcat ctataaccag gcgccagaac cttatggcgt cgatcacggg acgaataccg
    19441 gatcgcccca cgtcgagcgc accatcaccc atccgcagga atgaaccatg gccgatgaac
    19501 cgtcccctgt tgcgcgcccg ctgcccggtg gcgatccaat gcccgaaagg cacgaaatcc
    19561 ttcacgagcg gggaatcgaa ccgatcggcg gcatgacgtc cgcgcggcgc aacaccgcgc
    19621 tgatgttcgc cggcacggca atggccctgg gcattctctg ggtgaattcc ggcacgggca
    19681 agcagtcctc gtctcgtgac ctcaccgcgc cggcaggttc agcccgggaa cgacctgata
    19741 tcgtcgctcg ggaaaccgtc gattatgccg cggtcgcgcc tcaacctcgg tcgctcgggg
    19801 cagccggcgc agaccccaat gcgccggtgc tcaatcctgc agctggcgta cccggccctg
    19861 acggccaaat tgttcccgcc atgcagccgg gcgcggcacc gagcgggacc agcgcccccc
    19921 ggccaacact tgccgagcag gcgcggcggt ccacactgat tgcctatgga ggtcgcgatc
    19981 tcggcgctgg ggctggcgct cccacagctg caggggctgt gggcgaaatg cctgacaacg
    20041 ctgccggggc gccagagagc ggcgagggaa ggacgcccaa cgcgctcgat ctcttgcggc
    20101 aaagttcgtc gattggggaa gcgcgggcct cgatgctgcc gaaccgcaac ttcctgatta
    20161 cagcgggcac gttgatcccc tgtatcctgc aaaccgcgat caattccgcg cagcccggtt
    20221 acacgtcctg cctgatcccg cgggacgtct attccgagaa tggccgggtc gtactcatgg
    20281 aaaagggcac gcgcgttctc ggcgaatatc gcggcggcat ccagcaaggg caaaatcgcc
    20341 tgttcgtcct ctggacgcgg gcggtcacgc cccagggcgt gcggatcgat cttgcctcgc
    20401 ctggctccga tgcgctcggc cgagccgggc ttgccggcgc ggtcgacagc tttttctggg
    20461 cgcgctttgg cggcgccctg ctcctctcgc tcgtcgatga tgccgcttat atcgccgggc
    20521 aagccgcctc tagcggcaat ggcaatttca acaatgtgac ccgggcgccg agcgaaggcg
    20581 cggcgatcgc cttgcagaac aatatc
//
