LOCUS       NZ_CP102666            20636 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Sphingobium sp. JS3065 plasmid pNIN1, complete sequence.
ACCESSION   NZ_CP102666
VERSION     NZ_CP102666.1
KEYWORDS    RefSeq.
SOURCE      Sphingobium sp. JS3065
  ORGANISM  Sphingobium sp. JS3065
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Sphingobium.
REFERENCE   1  (bases 1 to 307737)
  AUTHORS   Li,T., Xu,J., Xu,Z.-J., Xu,Y., Spain,J.C. and Zhou,N.-Y.
  TITLE     Molecular mechanism and evolution of 1-nitronaphthalene catabolism
            in Sphingobium sp. strain JS3065
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 307737)
  AUTHORS   Li,T., Xu,J., Xu,Z.-J., Xu,Y., Spain,J.C. and Zhou,N.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2022) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai,
            Shanghai 200240, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. v0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 05:42:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,942
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,751
            CDSs (with protein)               :: 4,751
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 40 of 122
            Pseudo Genes (incomplete)         :: 96 of 122
            Pseudo Genes (internal stop)      :: 12 of 122
            Pseudo Genes (multiple problems)  :: 23 of 122
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:10:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 54132
            Orig. end                         :: 74768
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102666.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20636
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="2"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20636
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20636
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10636](+)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10636
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     misc_feature    6..21
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.86"
                     /tool="antismash"
     misc_feature    350..364
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            complement(1117..2286)
                     /locus_tag="NUH86_RS22285"
                     /old_locus_tag="NUH86_22290"
     CDS             complement(1117..2286)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053629.1"
                     /locus_tag="NUH86_RS22285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22290"
                     /product="histidine kinase"
                     /protein_id="WP_044663494.1"
                     /transl_table=11
                     /translation="MAEILLLSIDRMLAQRIGDAMGQRVDVRMVQSLDAEPLTRPGVIV
                     MDRSALPSDRSLAASISAVADQAAGRSIVLATDEQDSEQILHAVRAGAKDVIARDAEGA
                     EIANVLSRLLNATIAQQSTGGRLTLVLGVDQEATAMVASDMALIRSADRQPSLLIDCSL
                     PTSAAAAYLDLDVTYGLASAIADIERLDASLLSSALARHAASGLGLLTFDGGTGAEPAG
                     IAPTDIAALVRLLRACSSEVVFCAGSLRHGGLLRELALLADQVDIVCAQSIRELEAARR
                     LLDRISPDHATLERMRLLVWDHQPTVLLDGRRMADALGTGSMMPLPVDRAQSRNALNAG
                     QPLGIAPDGGAYQQALRRACGARPHQAKGALGAIQQLRRGVLRKLERRP"
     gene            complement(2288..3478)
                     /locus_tag="NUH86_RS22290"
                     /old_locus_tag="NUH86_22295"
     CDS             complement(2288..3478)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836656.1"
                     /locus_tag="NUH86_RS22290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22295"
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="WP_323749050.1"
                     /transl_table=11
                     /translation="MALTAPLLASNSAWAQDGETISVRQQRTFSYPSPIGRIAIEKEGV
                     VSVAAPSAKSLRITGIGPGEVTVNVYGINGRLIAQTPVSVVAAAGSTGGDLKALPASFS
                     TRGEQVVAVDVQFAAISASTLKALGFNFSKLSGDLQGAIVSPSTLSSYSMSPNLSLEST
                     APIQNAFNLFLSAPNRGIGAVLSALSSNGLSQLLAQPTLLVRSGEQASFLAGGEFPVPV
                     PQSSGGAGSTISIQYKEFGVRLSVAPFVLSKDRIVLKIAPEVSELDYNNGVQLQGYVVP
                     GIRRRSAETTVELGSGQSFVIAGLSYSSSNVSKEKTPLLGDLPILGAFFKRQQTQKERQ
                     ELIIVATPRLVGPVDAKDAPPLPGSATASVDPGLADMVTGADGVEQHAAPFGVVRR"
     gene            complement(3502..4392)
                     /gene="cpaB"
                     /locus_tag="NUH86_RS22295"
                     /old_locus_tag="NUH86_22300"
     CDS             complement(3502..4392)
                     /codon_start=1
                     /gene="cpaB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819613.1"
                     /locus_tag="NUH86_RS22295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22300"
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="WP_323749046.1"
                     /transl_table=11
                     /translation="MTVFVGLMVAACFLVLGIRELTRTPPSPAQAASPSRQAAATAPSP
                     VFLSAVRPIRTGETITADMVRNRPGDPQRYPYAATAPEVIGKVATRDIAANMLVARDAV
                     GAETKLAIRVPIGMRAVSIDTTAEIAVAGLVRPGDRVDVQVIYPGADAISGARGMGRSR
                     AETLLQMVQVLAVGELVVGTNQKTGPDGQQSTPQPPARTVTLALTPEQVSTLSLAKSTG
                     GLYLSLRNPIDDQKVAVAAMTSRPSLAAPGERDATAPPPRAAAQPKRAARQPSHAIELL
                     VGDRREVIYSGSTAR"
     misc_feature    complement(4365..4385)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.91"
                     /tool="antismash"
     misc_feature    complement(4571..4585)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.62"
                     /tool="antismash"
     misc_feature    4769..4783
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.45"
                     /tool="antismash"
     gene            4802..5821
                     /locus_tag="NUH86_RS22300"
                     /old_locus_tag="NUH86_22305"
     CDS             4802..5821
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /locus_tag="NUH86_RS22300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22305"
                     /product="EAL domain-containing protein"
                     /protein_id="WP_267253129.1"
                     /transl_table=11
                     /translation="MIDDGAAAVELAEFLRLLGHSATAWEAWTEDRCFDLVMVDTADQG
                     DWETHLAIAAARGVSWAILLVDDGTDIPAVRNVGGLRVIGTLMKPLTPVRVAAFLRNYG
                     NGSDGSVGQRAGDGPVRMPGRFIFRTKHALRGDAVVGYEAVAWQEGAGASPDHDSFAGT
                     IWAAEAALGLAGRLARARAKVPVAFSCSAAIFADAQFVREMQLLTRYARVDPGGLLVDV
                     ALATSTLRALDLSEAAERCAAAGFHVALGRRGEAAESWEPWTMLSLSEIRLDGCGAALP
                     PGEERALQREIVALCQQRGIRTTIGRIDSEADLRDARDTGADQGLGAYWGAPLRDVIL"
     gene            5818..6990
                     /locus_tag="NUH86_RS22305"
                     /old_locus_tag="NUH86_22310"
     CDS             5818..6990
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NUH86_RS22305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NUH86_22310"
                     /product="hypothetical protein"
                     /protein_id="WP_048574864.1"
                     /transl_table=11
                     /translation="MRDELRTWGAPRGVYLPVLTFSVVGIAGTAQFLPALTPLGAACAG
                     ALMALRRRLWPLIGIACLGLLMIMGLVARAPGHHWFLVAMLLMGALLPYRRMPAAVFLP
                     RPVWAICLGGAMASICLSVSVGPDHAAALIALGNVLAGALGAALVRQLALSNGGFPVAR
                     DMSVLDVSRDLLLGRVTSGMLHDLAQPLNVISMANGNLGYIMESLEMPPAQRAQLDERL
                     NRIAAQTEHAASILNLFRWFGRDREGAMLNVKSALQQAVAATRSNVRHGGVGVRLGGDA
                     LDYPLAGRHGLLEMMTVAALLSSFGTFVLADGSKIKGLVHVHAELSPSFIVIAIWCEDE
                     TARPCPAGPIDQTTLWLLEQIARAGDGDFRRAPEKHGLAQFTIRLARDDI"
     gene            complement(6954..8831)
                     /locus_tag="NUH86_RS22310"
                     /old_locus_tag="NUH86_22315"
     CDS             complement(6954..8831)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="NUH86_RS22310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22315"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_082165917.1"
                     /transl_table=11
                     /translation="MLERFQHLTGGNLAALPGRRASICLAVILIATLSLTPIFGRAPAA
                     TLVFFLAWLLKRHWALPRMLLIFAAFALAHMQWAGGLQALAAACVFLVAHGLRKWVRQS
                     ADATLLACCLIFPVILVAGWAPLEEALRWAAGFALGAAVADLAVAAALQDKRISPDGET
                     AYLAPIAALAAIINILTIVILDIMVGALARSVDDKLALTFAFIGERLTLPAFLILALLL
                     QLYSRQLARALEARAEVTPASLLPGENAAGNAVAQDSGSVAYEENRGALVDASGTPAFR
                     SISGICFDAHAGRLVFCEDAASHPGSLQIHPADASRVIESVGRGDTILTFRARTGPDFE
                     PMLLAMSCRTGKWRWESGILLHRGDQRHYSMALAQRARHADLGARIMAIAHELRQPLFT
                     ISVAAESQRLMLARADRSDRSTGQIAERAERIAEQVIRATAIIEQILSYGRTSANSEAE
                     LDVAETLRRSCAFLAPLLEERAIELDFSIEDGRHCTIMSPVELEQVFVNALQNAADSIV
                     SRRREGWTGTGRITCSVATCDGLVSCIIADNGAGVSPRHAEAAFNAFFSTKADEGTGLG
                     LFITREIILRAGGNVSLRPSLHEGALLAIELPATGHADQMSSRASRIVN"
     gene            complement(8824..9231)
                     /locus_tag="NUH86_RS22315"
                     /old_locus_tag="NUH86_22320"
     CDS             complement(8824..9231)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688409.1"
                     /locus_tag="NUH86_RS22315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22320"
                     /product="response regulator"
                     /protein_id="WP_048574866.1"
                     /transl_table=11
                     /translation="MNETLPHEALVLIIDDVPEIVDELVTMLSLLDIPAIGAGSLPAAM
                     AALESAPLLRAIACDVRLARESGLEILELIARHPHLSTRAYGFVFMTGDSMRPEVTLAH
                     KEYHLLSKPVQPRAFIEILQRMLAAPDASNA"
     gene            10001..10636
                     /locus_tag="NUH86_RS22320"
                     /old_locus_tag="NUH86_22325"
     CDS             10001..10636
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012967.4"
                     /locus_tag="NUH86_RS22320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22325"
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="WP_048574867.1"
                     /sec_met_domain="Autoind_synth (E-value: 2.6e-12, bitscore:
                     40.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVNIVCDRKALPAACANCWQRDSASCDLVPVDAITELPFDPDEYD
                     DKFAEYLVVRDPLTNRRMGSLRLLRTDRPHILGSVFPDLCDTPAPSGLSIREVTQLCIS
                     PGLRLRERRMVSRQLASALVEYGLLTGITAYTTVMELVWSRQLMSMGWDCEPLGLGRPV
                     GDTLLGAFQVHIDASTVGGLRAAGCYVPGKLEIVERDNQASLFDSLAA"
     gene            complement(10836..11351)
                     /locus_tag="NUH86_RS22325"
                     /old_locus_tag="NUH86_22330"
     CDS             complement(10836..11351)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444644.1"
                     /locus_tag="NUH86_RS22325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22330"
                     /product="MucR family transcriptional regulator"
                     /protein_id="WP_082165919.1"
                     /transl_table=11
                     /translation="MVSIDDEKGSLVSLTANIVSANVSNNPMAVGDLCEMIKSVHSALS
                     KLESPEEEKTAKRDPAVPIRSSIKEDYIVCLEDGKKLKTLKRYLRARYQMTPEEYRAKW
                     GLPADYPMAAPSYTNQRRELAHKIGLGLRAEPKGKRTATKLPSPVEKDAPKPPATSSRR
                     KSGRAAKV"
     misc_feature    complement(11431..11444)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.45"
                     /tool="antismash"
     misc_feature    11618..11630
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.4"
                     /tool="antismash"
     gene            12100..12486
                     /locus_tag="NUH86_RS22330"
                     /old_locus_tag="NUH86_22335"
     CDS             12100..12486
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NUH86_RS22330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NUH86_22335"
                     /product="hypothetical protein"
                     /protein_id="WP_146001553.1"
                     /transl_table=11
                     /translation="MSIMLLQLLAFSFLTLVTWVAYGDAGLLRVGERLVLFRAVLELGQ
                     SVSIVPGTCDLLDWCRDCSAIVAVLSVIGLARKAWLGLVAIDEEGSAARKAISASGCEI
                     IDMKAWKMRARVVGPASNVRNHRR"
     misc_feature    complement(12796..12811)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.04"
                     /tool="antismash"
     gene            12820..13347
                     /locus_tag="NUH86_RS22335"
                     /old_locus_tag="NUH86_22340"
     CDS             12820..13347
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /locus_tag="NUH86_RS22335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22340"
                     /product="thermonuclease family protein"
                     /protein_id="WP_082165933.1"
                     /transl_table=11
                     /translation="MLGAPPRRRHAKVRPFRRRRSKQGARSRGVLSLVAPAIIAGLAIG
                     LVVTQLEGRLPDWSLTGHDPTSEVGCVSPQVLDGDTLRCGARRIRLSSIDAPELPGHCR
                     PGRACTPGDPYASTGNLRSLVVGQAVSCRQIDTDHYGRTVARCAVQGRDLSCAQVQGGF
                     AVERYGALLCGP"
     gene            13636..15939
                     /locus_tag="NUH86_RS22340"
                     /old_locus_tag="NUH86_22345"
     CDS             13636..15939
                     /GO_function="GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1051:
                     TonB-dependent siderophore receptor"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159983.1"
                     /locus_tag="NUH86_RS22340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22345"
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="WP_267253130.1"
                     /transl_table=11
                     /translation="MSLSRLRASLASAAPTYLALSCFGLAASPAMAQDSGAQEAPAARL
                     GGVTVTETALDEQGIKVDRVSSPKFTQPLQDTPQTIQVISKELFNQQGATTLTEALRNS
                     PGVGTFYAGENGNTTTGDAIRMRGFDTSNSIFVDGVRDLGSISRDIFNTEQVEVTKGPA
                     GTDNGRTAPTGAINMVSKRASQEALLAGAVSVGVDGQTRATADINQPLEALPNAAFRLN
                     AVWQDSDVAGRDHVNNKRLGLASSLGLGLGTETRAFLNFLYVDQDNIPDGNVPTIGVKG
                     WQPQAGLEPLVGHPVDSTNFYGTRRDHDNVTAKMASLILEHDFSDSVKLTNIARWGETE
                     QDYLLTAFMSTGANITAGDPADLSTYVMTRGNPTIRDARNRILTDQLNLRADFATGSVE
                     HSLSVGVEITGEKQVIYGNSTTGALPVANLYNPNWNDTSAFGYARNGALSRGKTNTQAF
                     YAFDTAKFFDGQLLVTGGVRVDHYKTSYTSTALCNNGTGRGAVPCGGQPIDSIVTTADL
                     SASDTLFNWKLGAVFKPAEPLSLYVNYALSQQPPGGDNFTLATGDSANNPNLDPQKAKT
                     IEAGVKWSALNGMLAVNAAVFQTKVLNEINGSDPSDVQSGSKRVRGVELSVVGNITEDW
                     TISAGYSHQKTKVTNGSAIAVDGSYNLTYAPDDSFTSWTTYRMPFGLEVGGGVRYVSGL
                     HRGTDGAVGTPERTEGYTVVDAILSYALTDNVKLRVNAYNLFDKEYVVAINKSGYRYTP
                     GQAQTFLFSADFRF"
     gene            16007..16684
                     /locus_tag="NUH86_RS22345"
                     /old_locus_tag="NUH86_22350"
     CDS             16007..16684
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /GO_function="GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2OG-FeII_Oxy"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344076.1"
                     /locus_tag="NUH86_RS22345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22350"
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="WP_196227543.1"
                     /sec_met_domain="2OG-FeII_Oxy (E-value: 6e-07, bitscore:
                     24.2, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLHIPDVLTGEEVAEFRRRLDASDWTDGLETVGPQGARVKHNQQ
                     LPDASPLRAELGQAVLAAFRRTPLFFAAALPRKILPPRFNRYAGGGEYGFHIDGAVMSL
                     GEEEQLRSDISCTLFLSDPDDYDGGRLIISDTYGEHSVALPAGDAIIYPSSSLHRVEPV
                     TRGERLASFLWIQSLIRDDRQRRMLFELDTSIQKLTLDGADEDAVLQLTSVYHNLLRLW
                     SET"
     gene            complement(17205..17738)
                     /locus_tag="NUH86_RS22350"
                     /old_locus_tag="NUH86_22355"
     CDS             complement(17205..17738)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NUH86_RS22350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NUH86_22355"
                     /product="hypothetical protein"
                     /protein_id="WP_196227544.1"
                     /transl_table=11
                     /translation="MSLIYVTPRPFLVYYLIVFCIAMHPMAGYATKGPLMDRVEYPQAC
                     HAALLRAPPWIGRLSHQDSDRQMRTSVEPTHWQLELAQADMEMAADERERALSDICCLV
                     RRHEIRLRELISHLLQDGDGSQRSSTVEKSDSINTKASPVEDIHCSLLAHVAFASKILR
                     IPENRTMAEAINDH"
     misc_feature    17736..17751
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.02"
                     /tool="antismash"
     misc_feature    17736..17754
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.73"
                     /tool="antismash"
     misc_feature    complement(17739..17754)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.19"
                     /tool="antismash"
     misc_feature    17865..17879
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.36"
                     /tool="antismash"
     gene            17883..19079
                     /locus_tag="NUH86_RS22355"
                     /old_locus_tag="NUH86_22360"
     CDS             17883..19079
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /locus_tag="NUH86_RS22355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NUH86_22360"
                     /product="EAL domain-containing protein"
                     /protein_id="WP_267253131.1"
                     /transl_table=11
                     /translation="MSFGLAARKEAFERLRLEMHRIARRPFVTELESAGVIEAVIEWEA
                     GEGRPDHLIHRLACNMLSHPIVSESGCFYVVPEIGGAALDREHGDIDSAKAGAYRQLDS
                     SSVKIQQPWHLFRYREDMSRAAELLQKIDRGEAMLVWQAIVSPADRAPLLYFEGLLRVS
                     GEDGALFSCEANIRAAERVGLAPELDRRLVSRALDQLETDDDVRMGVNISARSASLHRY
                     GRDAGWRELRSRLEREPELARRLVIEITETADFASLDEAAEFVSFMQHLGCQVAIDDFG
                     AGRGSIIQYGALCADIIKIDGSFVQQAGQNEKGHATFRHLVGLARALAPQVVVEGVETA
                     DQAELARGIGIDWFQGYHFGRPSPTRALGVSADARAIMALRNFQDRFRAFSKAGGALA"
     misc_feature    complement(19340..19353)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.7"
                     /tool="antismash"
     misc_feature    complement(19341..19354)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.45"
                     /tool="antismash"
     misc_feature    complement(19342..19355)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.83"
                     /tool="antismash"
     misc_feature    20535..20550
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.11"
                     /tool="antismash"
ORIGIN
        1 tgtgaacgct gttggcgatg aaacggcgga tcgcgcgaac gtcgttctgg aggcccgcaa
       61 agcccagaag catctccagc cgggcgaagg catcgcgcgg cgtattgccg tgcagggtcg
      121 ccatcgagcc gtcatgaccc gtcgacatag cctgcagcat ctcgaccgct tcgacgcccc
      181 ggacctctcc caggatgatg cgatcaggac gcatgcgcag cgcgttgcgg accagttcgc
      241 gcgcgctcac ctcgcggctg ccgtcatgat tgggcgcccg cgtttccagg cggacgacat
      301 gctcctgccg cagctgcagc tcggctgcgt cctcgatcgt gatcagccgc tcatgatcgt
      361 cgatgaaggt cgagagcatg ttgagaagcg tcgacttgcc cgcgccggtg ccgccgcaga
      421 tgagaatgtt cagccgggac cgcaccgctg ctgcaagata ggccgccatg tcggcgctca
      481 tggcctgccg ttcgacgaaa tcctcgatcg tgcgcgcctg caggcggaac ttgcggatcg
      541 acaggagcgg gccgtcaagc gcaatggggg ggatgacgat gttgacacgc gagccatcgg
      601 gcagccgggc atcgacatag ggcgatgcct catcgacgcg tcggccaata ggcccgacga
      661 ttcgctggat gatgttcatg acatgggcgt catcgcgaaa gcgggtttcc accttttcaa
      721 gaaggcctgc gcgttcggcg taaatggcgt tgggtccgtt gacgataatg tcatcgatgg
      781 tcggatcgcg cagcagtggc gcaagcgggc caaggcccac gacctcgtct tcgacatcct
      841 cgatcagcag caggcgctcg gctgaattga gcgcgagctg cccgcgccgg cttctgctgg
      901 tgatgacgag ttcgatttct tcgcgcaggg cgcggcgttc gagcgattcg gccgtattgc
      961 cgcgcgcttc gagctgctcg atgacctgtt ggcaggtctg ggccttggcg gcctggtagc
     1021 tgtcgctcat gcgctgggcg gccgagggcg cgatggtcgt gggacgcgcg gttcccgggg
     1081 ttcccgcgac gggagcggtt tcttcggtga gaaggctcat ggccggcgct ccagcttccg
     1141 caggacgccg cggcgcaact gctggatggc gccgagcgca ccctttgcct gatgaggccg
     1201 agcgccacag gcccggcgaa gggcctgctg ataggcgccc ccgtccgggg cgatcccgag
     1261 cggctgtccc gcgttgaggg cattgcggct ttgcgcgcga tcgaccggca ggggcatcat
     1321 ggatcccgtg cccagcgcat cggccatgcg gcgcccgtcg agcagcactg ttggctgatg
     1381 atcccagaca agcagccgca tccgctcgag cgtggcgtga tcggggctga tgcggtcgag
     1441 gagacgacgc gcggcctcga gttcgcggat cgactgggcg cagacgatgt cgacctggtc
     1501 ggcaagaagc gccagttcgc gcagcaggcc gccgtgacga aggctccccg cgcaaaagac
     1561 gacttcgctc gagcaggccc gcagcagccg gacgagcgcc gcgatatcgg taggggcgat
     1621 gcccgcgggt tccgctccgg tgccgccatc gaaggtaagc agtccaagac cgctggccgc
     1681 gtggcgggcg agcgcgctgg aaagcaagct tgcatcgaga cgctcgatgt ccgcgatggc
     1741 agaagccagg ccataggtca cgtcgaggtc gagataggcc gcagcggcgc tggtcgggag
     1801 cgagcagtcg atcagcaggc tgggctggcg gtccgcgctc cggatgagcg ccatgtcgct
     1861 cgccaccatg gcggttgctt cctgatcgac ccccaggacc agcgtgagcc gtcccccggt
     1921 gctttgctgg gcgatcgtcg cgttgagcag gcgggacaga acattggcga tctccgcccc
     1981 ttcagcgtcg cgcgcgatca catccttggc gcccgccctg acggcgtgca gtatctgttc
     2041 gctgtcctgt tcgtcggtcg caagcacgat cgagcggccc gccgcctgat ccgcaacggc
     2101 gctgatcgat gcggcgaggg accggtcgga cgggagcgcc gagcggtcca tcacgatgac
     2161 gccggggcgg gtgagaggct cggcgtcgag cgactggacc atcctgacat caacgcgttg
     2221 ccccatggca tcgccaatgc gctgggccag catccgatcg atggagagca gcaggatttc
     2281 ggccatgtca gcgcctcacc acgccgaagg gggcggcatg ttgttcaacg ccatctgctc
     2341 cagtgaccat gtcggcgagg cccggatcga ccgacgccgt ggcgcttccc ggcagcggcg
     2401 gcgcatcctt ggcatcgaca ggcccaacca ggcgcggcgt ggcgacgatg atgagttcct
     2461 gccgttcctt ctgggtctgc tggcgcttga agaaggcgcc gagtatcggc aggtcgccga
     2521 gcagcggtgt tttctccttg gagacgttgc tgctgctgta gctcaggcct gcaatgacga
     2581 agctctggcc gctgcccagt tccaccgtgg tctcggccga tcgccgtcgg atgccgggaa
     2641 cgacatagcc ctgaagctgg accccgttat tatagtcgag ctcgctcacc tctggtgcga
     2701 tcttcaggac gatgcgatcc ttggaaagca caaagggggc caccgataga cgaacgccaa
     2761 attccttgta ctggatggaa atggtgctgc cggctccgcc cgacgattgc ggaacgggaa
     2821 ccgggaattc gccgcccgca aggaagcttg cctgttcgcc cgagcgcacg agcagcgtgg
     2881 gctgcgccag aagctgggaa aggccattgg aggagagcgc gctcaatacg gcgccaatgc
     2941 cccgattggg cgcggaaagg aaaagattga acgcgttctg gatgggcgct gtggattcga
     3001 gcgacagatt gggcgacatg gagtagctgc tcaacgtgct gggcgagacg atcgcgccct
     3061 gcagatcgcc gctcagcttg gagaagttga aaccaagggc cttgagggtc gacgcggaaa
     3121 tggccgcaaa ctggacgtcg accgcgacca cctgctcgcc gcgggtcgag aaactggcag
     3181 gaagagcctt gagatctccg ccagtcgaac cggcagctgc gaccacgctt acaggcgttt
     3241 gggcgatcag gcggccgttg attccataga cattgaccgt cacctcgcca ggaccaatgc
     3301 ccgtgatgcg gagcgacttt gcggacgggg cggcgaccga cacgacgcct tccttctcga
     3361 tcgcgatgcg gccgatcggc gatggatagg agaaggtccg ctgttgccgg acagagatcg
     3421 tttccccgtc ttgcgcccaa gcggaattgc tggccagcag gggagcggta agggccatca
     3481 gcccggcgat acgccagttg ttcatcgcgc agtgctcccg gaatagatga cctcgcggcg
     3541 gtctcccacg agcagttcga tggcgtgcga cggctgccgc gcagcgcgtt tgggctgcgc
     3601 ggcagcgcgc ggtggcggtg ctgtcgcgtc gcgctcgccc ggtgcggcga gcgagggcct
     3661 ggaggtcatc gcggccacgg cgaccttctg atcatcgatg ggattgcgaa gcgaaagata
     3721 gaggcctccc gtgctcttgg cgagcgacag ggtcgagacc tgttcgggcg tgagggcaag
     3781 ggtgacggtg cgggcgggag gctgcggcgt gctctgctgg ccgtcggggc cggtcttctg
     3841 gtttgtcccg accacaagct cgccgacagc gaggacctga accatctgga ggagcgtttc
     3901 ggcgcggctt cggcccatcc cgcgtgcgcc gctgatggcg tcggcgcccg gatagatcac
     3961 ctggacatcg acgcggtctc ctggccttac cagcccggcc accgcgattt cggcggtggt
     4021 gtcgatgctg accgcgcgca tgccgatggg cacccggata gccagcttcg tctcagcgcc
     4081 cactgcgtcg cgcgccacaa gcatgttggc tgcgatgtca cgcgtggcga ccttgccgat
     4141 cacttcgggg gctgtcgcgg catagggata gcgctgcgga tcgcctggcc tgttgcggac
     4201 catgtccgcg gtgattgtct ctccggtcct gatgggccgc acggccgaga gaaatacagg
     4261 cgacggcgcc gttgccgcag cttggcggga cggactggca gcctgtgccg gagacggcgg
     4321 tgtgcgcgtc agctccctga tgccgaggac cagaaaacag gccgcaacca tcagcccgac
     4381 gaaaaccgtc actctagccc gcattcccag gcgcattcgg cccccctttg cgcatacgca
     4441 aaaatccgcc cgacgggcat gaatattacg aatcaataga acggtcgccg ttcagcacgg
     4501 ttaagtaacc gttcagcgca gaatcaggga atggagatgg aaccattttg attgtaacgc
     4561 cggaattgcg caaaaatgac ggtcattcca tgggatttgc gtatatagaa agcatgattg
     4621 cgtccggatg ctatatgacc gtttcccaat ccttgacgtt cgggacatga aaatgggtat
     4681 tggggagcat tcatgccgtt cctgttgcaa gctggggctt ttcaaggtca cggcttggac
     4741 atccctctaa acctgagcat tgaaggccat ttttctgaaa agcgggcttg atatcctcct
     4801 gatgatcgat gacggggcgg cggctgtcga actcgcggaa ttcctgcgcc ttctgggcca
     4861 tagcgcaacg gcgtgggaag cgtggaccga agaccgttgc tttgatctgg tcatggtgga
     4921 taccgctgac cagggagatt gggagacgca tcttgcgatc gccgctgcaa gaggcgtgtc
     4981 atgggccatc cttctggtgg atgacggaac cgatatccct gccgtgagga acgttggcgg
     5041 ccttcgcgtc atcggaacgc tgatgaagcc gttgacgccg gttcgcgtcg ctgcctttct
     5101 ccggaattac gggaatggga gcgatggttc tgtgggccag cgggccgggg acggcccggt
     5161 tcgaatgccc ggacggttca tattccggac gaagcatgcg ctgagggggg atgctgttgt
     5221 cggctatgaa gcagtggcat ggcaggaggg cgcaggcgcc tcgccggatc atgacagctt
     5281 tgcagggacc atctgggcgg cagaggcggc ccttggcctt gccggtcgcc ttgccagggc
     5341 gcgagcgaag gtgccggttg ccttttcctg ctcggcggcc attttcgccg acgcgcaatt
     5401 tgttcgcgaa atgcagctgc tcacgagata tgcgcgggtg gatcccggtg gccttcttgt
     5461 ggacgtcgcg ctcgccacaa gcacgcttcg tgccttggac ctgagcgagg cggcggagcg
     5521 ctgcgcggca gctggattcc atgtcgcgct gggcaggcgc ggtgaagccg ctgagagttg
     5581 ggagccgtgg accatgcttt cgctgagcga gatcaggctg gatggctgcg gcgccgccct
     5641 gccgcctggg gaggaacgcg cgctacagcg tgaaatcgtg gcgttgtgcc agcagcgggg
     5701 catcaggacg acaatcggac ggatcgattc ggaggcggac ctgcgggatg ctcgcgacac
     5761 gggcgccgat cagggattgg gagcctattg gggcgcgccg ctgcgcgatg tgatcttgtg
     5821 agggatgagc tgcgaacatg gggcgcaccg cgcggcgtct atctgcccgt cctgaccttt
     5881 tccgttgttg gcattgcggg cacggcgcag ttcctccctg ccctgacgcc gctcggggcg
     5941 gcatgcgcgg gagccttgat ggcgcttcgc cgccggcttt ggccgctgat cggtattgcg
     6001 tgccttggtt tactcatgat catgggcctg gtcgctcgcg cgccaggcca tcactggttc
     6061 cttgtggcca tgctcctgat gggggcactc ctgccctaca ggaggatgcc tgcggccgtg
     6121 ttcctgccgc ggcccgtgtg ggcgatctgc cttgggggcg ccatggcatc catttgcctg
     6181 tcggtcagcg tggggccgga ccatgccgca gccctgatcg ctctgggcaa cgtccttgct
     6241 ggggccttgg gcgctgcatt ggtgagacag cttgcattgt ccaatggcgg cttccccgta
     6301 gccagggaca tgagcgttct tgacgtctcg cgagatctcc ttctggggcg ggtgaccagc
     6361 ggcatgcttc atgacctggc gcaaccgctc aacgtcatct ccatggctaa cggcaatctg
     6421 ggctatatca tggaaagtct ggagatgccg cctgcgcagc gagcgcaact tgacgaaaga
     6481 ttgaaccgga tcgccgcgca aacggagcat gcggcctcca ttctcaatct gttccgctgg
     6541 tttggccgcg accgggaagg ggcgatgctg aacgtcaaga gcgcgctgca acaggcggtg
     6601 gccgccacgc gctcgaatgt tcgccatggc ggtgtcggag tgcgcctggg cggcgatgcg
     6661 ctcgattatc cccttgccgg gcggcatggc ctgctggaga tgatgacggt cgcggcgctg
     6721 ttgagttcat ttggaacatt cgtgctggcc gatggaagca agatcaaggg tctcgtccat
     6781 gtccatgccg aacttagccc gtcattcatc gtgattgcta tctggtgcga ggatgagacc
     6841 gcaaggcctt gccctgccgg tccgatcgac cagacaacct tatggctgct cgagcagatt
     6901 gcgcgcgcgg gcgacggcga tttccgccgc gcgccggaaa agcatggcct cgctcagttc
     6961 acgatccggc tggcgcgcga cgacatctga tcggcatggc ccgttgcggg tagctcgatc
     7021 gcgagcagcg cgccttcgtg caaggagggg cgaaggctca cattgccgcc cgcgcgaaga
     7081 atgatctcgc gcgtgatgaa gagcccgagc cctgtccctt catccgcttt ggtggagaag
     7141 aaggcgttga aggcggcctc ggcatggcgg gggcttacgc ccgcgccatt gtccgctatg
     7201 atacaggaaa caaggccgtc gcaagtcgct acggaacatg tgatgcgtcc tgttccggtc
     7261 catccttcgc gccgcctgct gacgatgctg tcagcggcgt tttggagagc gttgacgaaa
     7321 acctgctcca gctcgaccgg cgacatgatg gtgcagtgcc ttccatcttc tatcgagaaa
     7381 tcgagttcga tcgcgcgctc ttccagcagg ggagccagaa acgcgcagga tcggcgcaat
     7441 gtttctgcca cgtccagttc ggcttccgag tttgcgctgg tgcggccata actgaggatc
     7501 tgctcgatga tcgccgtggc gcggatcacc tgctcggcga tgcgctccgc ccgctccgcg
     7561 atctggcctg tgcttctgtc ggacctgtcc gctctggcga gcatgaggcg ctggctctct
     7621 gccgccaccg agattgtaaa cagcggttgg cgcagttcgt gggcgatggc catgatgcgc
     7681 gcgccaaggt cggcatggcg cgcccgctgc gccagcgcca tggaatagtg gcgctgatcg
     7741 ccgcggtgga ggaggatgcc ggattcccat cgccattttc ccgtccggca gctcatggcg
     7801 agtagcattg gctcaaaatc cgggcctgtc ctggcgcgga atgtgaggat cgtgtcgccg
     7861 cggcccacgc tttcgatcac tcggctcgcg tcggcgggat gaatctgcag gctgcctggg
     7921 tgtgaggcag cgtcttcgca aaaaacgagc cttcccgcgt gcgcatcgaa gcaaatgccc
     7981 gaaattgatc ggaaggcggg cgtcccggag gcgtccacca aggcgccgcg attttcctca
     8041 taagccaccg atccgctatc ctgtgcgacc gcgttccctg cggcattctc tccgggcagg
     8101 agcgaggcgg gcgtaacctc tgctcttgcc tccagcgcgc gcgcgagctg gcggctgtac
     8161 agctggagca agagggccag gatcaaaaat gccgggaggg tcagacgctc gccgatgaag
     8221 gcgaaggtga gggcaagctt gtcatcgacc gatctcgcca gggcgccgac cattatgtcg
     8281 aggatgacaa tggtgaggat gttgatgatg gcggcaagag cagcgatcgg cgcgagatag
     8341 gccgtctccc cgtcaggcga aatgcgcttg tcttgcaagg ccgcagcgac agccagatcc
     8401 gccacggccg ctcccagtgc gaaaccagcc gcccaacgca gcgcctcttc gagtggcgcc
     8461 cagcctgcga cgaggatgac tggaaaaatc aggcagcacg ccaggagggt cgcatcggcg
     8521 gattggcgaa cccatttacg caatccgtgg gccaccagaa aaacgcaggc cgcggccagg
     8581 gcctggaggc cgcctgccca ttgcatgtgc gcgagcgcaa aggccgcgaa gatcagcagc
     8641 atccgcggca gcgcccagtg gcgtttgagg agccaggcca ggaaaaacac cagcgtagcg
     8701 gcgggcgccc ggccgaatat gggtgtcagg ctcagcgttg caatgagaat gactgccaga
     8761 catatggacg cgcggcgtcc aggaagagcc gcgaggttgc caccagttaa atgctgaaac
     8821 cgctcaagca ttcgaagcgt ccggggcagc cagcattcgc tgcaaaatct cgatgaacgc
     8881 gcgtggttgc accggctttg agagcagatg atattctttg tgcgcgaggg tgacctccgg
     8941 ccgcatggaa tctccggtca tgaacacgaa accgtaggca cgggtggata ggtgcggatg
     9001 tcgggcaata agctcgagga tttccaatcc cgattctctt gcaagcctga catcgcaagc
     9061 aatcgccctg agtaagggcg cgctttcgag cgccgccatg gcggccggca aggatccggc
     9121 cccaatggca gggatatcaa gaagcgaaag catggtgaca agctcatcga cgatctccgg
     9181 cacgtcatcg atgatgagca ccaatgcttc gtgcggcagg gtctcattca tattccgatc
     9241 ctttgcgaag gccgaaccct ccgtgccatc gtctagggca aagtgacgcg cgtttacaat
     9301 cacatctccc cgtcattgcg ggttgccgta gcgtctcgac gcaatggaag agggcggtcg
     9361 cgcgcgcaga ttgccgcgcc cgggaaggga aagcctggca gacgaaccat gctctaaaag
     9421 ttatgggatt ctcgatatgc tgagcgagaa gtcggatccg gagctgcggc tggcacgatg
     9481 tgcataagat cggcaaggaa cgggcatagt gctggcaata atgggcgagc tggggtgaat
     9541 cgggtgtgct gaccggccat cagctgcggc tcgtttatgc aaggttaagg ttgaccatgg
     9601 ctatcctggg ttaatttcct cttgggaccg ttttcgcctt gtcctctcga gccggttggc
     9661 gatagggggc gggaggctat ctcgagcaaa gaatttctgt tgaagatcaa ggctgtcgga
     9721 attgtatcgg gaccaggacg acctcttaac catgtgccgg acacgtgcgg gttggacgcg
     9781 acgaggccgg tttgaaggat gaagaagcaa ttggaaggcg ggctattcga gcggacggtg
     9841 cgctggttct tttgccttca agacgaacga cagatgggcg tgaccgaccg cggagcgctg
     9901 attttctcat gaattttagc gagtgaggcg gatgggaagt gctaatcgcc ctattgggct
     9961 atcttccggt ggcagcagtg cagaaattgc tgcgccacga ttggtcaaca tcgtctgcga
    10021 cagaaaggct ctgcctgccg cctgcgccaa ctgctggcaa agagatagcg cttcgtgcga
    10081 tctcgttccg gtcgatgcga taaccgagct tcccttcgac ccagatgagt atgacgacaa
    10141 attcgccgaa tatctggtgg tacgcgatcc cctgacgaat agacgcatgg gatctttgcg
    10201 gttgctgcgg acggatcggc ctcacatatt gggttctgta tttccagacc tgtgtgacac
    10261 gcctgcgccc agcggacttt ccattcggga ggtaacgcag ctgtgtatct cgccaggcct
    10321 gcgcctgcgc gagcggcgga tggtcagccg tcaattggcg agcgcgcttg tggaatatgg
    10381 gctgttaacg ggtatcacgg cctatacgac ggtgatggaa ttggtttggt cccggcagct
    10441 catgtccatg ggctgggatt gtgagccgct gggattgggg cgcccggtgg gggacacatt
    10501 gctgggtgcg ttccaggttc atatcgacgc cagcacggtc ggcggcttga gagcggcggg
    10561 ctgttatgtc ccgggtaaat tggagatcgt cgaaagggac aatcaggcga gcctgtttga
    10621 ctcgctggcg gcctgaatca aggctggcag cgtggctcat tggatcggat ccgatgagcg
    10681 gcaccaaaag gaaaagaaca ggattttggg ctaaagcgcc acaggctcgg gcgcccagca
    10741 agggccgtcg aatttcctgg ttcttgcggt ggctgtgggc ctctcagaac gctggctctg
    10801 ggaggccctt gggatcggaa cgaaaatcct tgcgcctaga ctttcgccgc acgccctgat
    10861 ttacggcggg aagacgtagc agggggcttg ggagcatcct tttccaccgg cgagggcagc
    10921 ttggttgcgg tccgcttccc ctttggctcg gcccgaaggc ccaggccgat cttgtgggcg
    10981 agttcccgtc gctgattcgt gtagctcggc gcagccatgg gatagtcggc gggcagtccc
    11041 catttggcgc gatattcctc cggggtcatc tgatatcggg ccctgaggta acgcttgagc
    11101 gtcttcagct tcttgccgtc ttcgaggcaa acaatatagt cctccttgat ggaggaacgg
    11161 ataggaacgg caggatcacg cttcgcggtt ttctcttctt ccggagattc cagcttggac
    11221 aaggccgaat gaacactttt gatcatttcg cacaggtctc caacggccat ggggttgttg
    11281 gaaacatttg ccgacacgat attagcagtc agtgatacga gagagccttt ttcgtcatcg
    11341 atacttacca ttttgcgccc caacttcttc gcagtcgaaa tatagaatca acgaatgcaa
    11401 gataacgatg ctgaccccat cttgcaaacc atttattaac atataattaa gcacagagat
    11461 atatatctaa cgacatgctc gccgccgcga tccgcacaga tatttgaaga ctttttaatg
    11521 ggttgcaaga ggggcgtaaa ctaaagggtg tttgcgccgt ctcgatagcc gcacgatgca
    11581 tcgcggggct gcccgttaat tccttcgaat cgaattcttg catcttgcag ctttcgctcc
    11641 atccccatcg ctgtccgcaa cgcctatgct gcgggtggac aagcggaagc gacaatggca
    11701 agtcctggta aagaatcgat tgatcgatgg ccggtgtgtc ggaggtcaga aagctcttca
    11761 aatgcgagac aggcgcgatt ctggcggcgt cggggcggaa cctggataca tgttgatttg
    11821 cggttcgggg cagccgaaat cagagttggc atgcattgat ccgccgtgat gctgcaggag
    11881 ttctcggcgg gacggccaag gcggacatgg cttggcggag atcgcggctg acatgctttg
    11941 caggatcgaa gggccggttt cggataggca taacctcgtt ggcgtggatt gcttgtgaga
    12001 cggaatatcg ctgcatcttt tgccaggccc ttttgctggg tcggcgtagc cggggcaggg
    12061 gcgggcatct ggctttttga cgtaccggcg ccttcgacct tgtccatcat gcttttgcag
    12121 cttctcgcat tttcgtttct gacccttgtc acatgggtcg cctatgggga cgcgggacta
    12181 ttgcgcgttg gcgagaggct cgtcctgttt cgtgcggttc tggagttggg ccaatcggtt
    12241 tcgatcgttc ctgggacctg cgatctgttg gattggtgcc gcgattgcag tgcaatcgtt
    12301 gcggtgttga gtgtgatagg gctggcgcgc aaagcctggt taggattggt agcgattgat
    12361 gaggaagggt cggcggctcg aaaggcgatt tccgcgtccg gctgcgagat tatcgacatg
    12421 aaggcttgga agatgcgggc gcgggtcgtc ggcccggcct cgaatgtgcg taatcaccgc
    12481 cgctgaaaga tgtgcaaact gccgggccag actatggcgt cggcggaggc tgcctgaccc
    12541 ggatgatgcc tgctcaggcg ccatcgccct gactggaaat cgcggcgtct gcttgtcgca
    12601 agcgaacgag ctgtcatctt acgagccgga aacgccgact ggatgtcgac ctgttggcca
    12661 caccgggcag attgcctgca gactgtcaga ggatgggccg agttgagcct catgtggacg
    12721 cgccaatgag cgatcatcgg gtcttgccac ggactggatg acagacggcg gcagcgtgta
    12781 gataagaccc tcatgaagaa tgatttcgat ttccgcagga tgctgggagc tccgcccaga
    12841 cggcggcatg ccaaagtcag gccctttcgt cgccgccgga gcaagcaggg cgccaggagc
    12901 cgcggggtac tttctttggt cgcaccagcg attatcgcgg gccttgccat aggcctggtt
    12961 gtcactcaac tcgaaggcag gctgcctgac tggtctttga ccggtcatga cccgacgtca
    13021 gaggtggggt gcgtcagccc acaggttctg gacggcgaca cattacggtg cggcgcgagg
    13081 cgcattcgcc tatcctcgat cgatgcgccg gagctgccgg gccattgccg gcccggccgc
    13141 gcttgcacgc ccggggaccc ctatgcaagc acaggcaatc tccgatcgct ggtggtggga
    13201 caggcggttt cctgccgcca gattgacacc gaccattatg ggcggacggt cgcacgatgc
    13261 gccgtgcagg ggcgggattt gtcctgtgcg caggtgcagg gcggcttcgc tgtcgagcgc
    13321 tatggcgccc ttctttgcgg gccgtgacat tgagcgggga aggcatggtt gagcccgcct
    13381 caggcacggc cttgttgcca tcttattgca gtgatcggca agcggcttgc catgagaagg
    13441 gcggttcatg cggtgccgca tcgatgcttc gtgcagcagt acgccctgaa aagttcgttc
    13501 agcgcgcttg gaaataacaa tcggcaagga tcggacgagc attcgcgacg ggagattagg
    13561 ggggttgcat tagatgcaaa tagctcgcat tagcggcgca tctgtttcca atccatgtcc
    13621 gaagggggtt attgcatgtc cttgtcgcgt ctccgcgcat cgctggcgtc cgccgctcca
    13681 acctatcttg cgctgagctg ttttggcctt gcagcatcgc cagccatggc ccaggattcc
    13741 ggcgcccagg aagcgcccgc cgcccggctt ggcggcgtca ccgtcactga gacggcgctc
    13801 gacgagcaag gcatcaaggt cgatcgcgtt tcgtcaccca aatttaccca gcctttgcag
    13861 gatacaccgc agaccattca ggtgatcagc aaggagctgt tcaaccagca gggcgcgacg
    13921 acgctgacgg aagccctgcg caacagccct ggcgtgggca ctttctatgc cggcgaaaat
    13981 ggaaacacga cgaccgggga cgcgatccgc atgcgtggct tcgacacctc gaacagcatc
    14041 tttgttgacg gtgtgcgtga cctgggctcg atctcccgcg atatcttcaa caccgagcag
    14101 gtcgaggtga cgaaaggtcc tgccgggacc gacaatggcc gtaccgcgcc aaccggcgcc
    14161 atcaacatgg tcagcaagcg ggcttcccag gaggcgttgc tggcgggcgc cgtttccgtt
    14221 ggcgttgacg gccagacgcg agcgaccgcc gatatcaacc agccgctcga ggcgttgccc
    14281 aacgcagcct ttcgcctgaa cgcggtctgg caggacagcg atgttgctgg ccgtgatcat
    14341 gtgaacaaca agcgactggg gctggcgtcg tcgctggggc tggggcttgg aacagagacc
    14401 cgcgccttcc tcaatttcct ttatgtcgat caggacaata tcccggacgg caatgtcccc
    14461 acgatcggcg tcaaaggctg gcagccgcag gcggggctgg agccgctggt cggacatccg
    14521 gttgattcga cgaattttta cggcacgcgg cgcgaccatg acaatgtcac ggcgaaaatg
    14581 gcgtcactga tcctcgaaca tgatttttcc gatagcgtga aactgacgaa catcgcccgc
    14641 tggggcgaaa cggagcagga ttatctgctc acggccttca tgtcgacggg agccaatatc
    14701 acggctggcg atcctgcgga cctgtccacc tatgtgatga cccgcggcaa tcccacgatc
    14761 agggacgcaa ggaaccgcat cctgaccgac cagctcaatc tgcgtgcgga ttttgcgacc
    14821 ggttctgtgg agcacagcct gagcgtcggc gtggagatca cgggtgaaaa gcaggtgatc
    14881 tacggcaaca gcacgacggg cgcgctgcct gtcgccaatc tctacaatcc caactggaac
    14941 gacacgagcg catttggcta tgcgcgcaat ggcgctcttt cgcggggcaa gaccaacacg
    15001 caggcatttt atgcgttcga caccgcgaaa ttctttgatg gccagctgtt ggtgacgggc
    15061 ggggtgcgcg tcgatcacta caagaccagc tacaccagca cggcgctgtg caacaacggc
    15121 acgggccgcg gagccgtccc ctgcggcggt cagccgatcg attccatcgt cacgacggcc
    15181 gatctgagcg cgagcgacac gctcttcaac tggaagctgg gcgcggtctt caagccggca
    15241 gaaccgctta gcctctacgt gaactatgct ctctcgcagc agccgccggg cggcgacaat
    15301 ttcacgctcg cgacgggcga tagcgccaac aatcccaatc tcgatccgca aaaggccaag
    15361 accatcgagg ccggcgtcaa atggtccgcg ctgaacggca tgttggctgt caacgccgcg
    15421 gtgttccaga ccaaggtcct caacgagatc aacgggtccg atccttccga cgtgcagagc
    15481 gggtcgaagc gggtgagggg tgtggaactc tcggtggtcg gcaatatcac cgaggactgg
    15541 acgatatcgg ccggttatag ccatcagaag accaaggtta cgaacggctc agcaatcgcg
    15601 gtcgacggca gctataatct gacctacgcg cccgacgaca gcttcacgtc ctggaccacc
    15661 tatcgcatgc ccttcgggct ggaggtcggc ggcggcgtcc gttatgtcag cggcctgcat
    15721 cgcggcaccg atggcgcggt gggcactccg gagcggaccg aaggatatac ggttgtggac
    15781 gcgatcttgt cctatgcgct gacggacaac gtcaagctgc gcgtgaacgc ctataacctg
    15841 ttcgacaagg agtatgtcgt ggcaatcaac aagagcgggt accgctacac gcctggtcag
    15901 gcgcagacct tcctgttcag cgccgacttc cgtttctgat tcctgtaggg caaaagacaa
    15961 tggcggggga tatcccccgc catttcttga tgtatcggac gcttccatgt tgctgcacat
    16021 tcccgatgtt ctgacaggtg aggaagtggc ggaatttcgt cgcaggctgg atgcgagcga
    16081 ttggacagac ggtctcgaga cggttggccc gcagggggcc agggtcaagc ataaccagca
    16141 gctgcctgac gcatcgccgc tgagggctga acttggccag gcggtacttg ccgcgttcag
    16201 gcggaccccg cttttcttcg ccgcggcctt gccgcgcaag atcctgccgc cgcgcttcaa
    16261 ccgctatgcc ggtggcgggg aatatggttt tcacatcgac ggcgccgtga tgagcctcgg
    16321 cgaggaagag caattgcgct cggatatctc ctgcaccctg tttctgagcg atcccgatga
    16381 ctatgatggc ggccggctga tcatcagcga cacttatggc gagcatagcg tggcgcttcc
    16441 cgcgggcgat gcgatcatct atccttcgag cagtcttcat cgcgttgagc ctgtaacccg
    16501 cggtgaacgg ctcgcctcct tcctctggat acagagtctc atacgcgatg atcggcagcg
    16561 gcgcatgctg ttcgaactcg acacatcaat ccagaagctg accctcgatg gcgccgacga
    16621 ggatgcagtg ctgcagctta ccagtgtcta tcacaatctg ctgcgcctct ggtccgaaac
    16681 ctgaacctgc ggtccggcgg cgcgttccga ttaacggaca ggaagaatgg ctgacgcgcc
    16741 gtgcgccgcc aactcagccc cgcgtcacca gccatcccag gcccaggaag gcaaggccaa
    16801 ggagcacagg tccactccag cgcgaaaccc gctgcaccag tctttcacac ctctcggcga
    16861 ggtccggcga atggtccggg gcataatcgt ccgcccggag caacgcatgg gcgggatggc
    16921 gcctgctttg gggcctgtgc gagcctgagc attcttcatc cgcaggaaga tgtgggggca
    16981 ttggccatcc atgttcgcgg tagtcgggca tcaagatccc tgttctcctg gcaaaaggct
    17041 tgggccgtat gtgtggatgg gagcggcatc atgttgagcc agggcaggcg gagaccggtc
    17101 cccctgcttg aggcgtgcag gtcggcgaca cgcatgaacg accattcatc atggtgagag
    17161 ctgccccgga actgtcccaa tcctcaccag catcaggtga cgcgtcaatg gtcattgatt
    17221 gcctcagcca tcgtcctgtt ttccgggatc cgtaaaatct ttgacgcaaa agcgacatgc
    17281 gccagtaacg agcagtgaat gtcttctaca ggacttgcct tggtattgat cgagtcgctt
    17341 ttttccacag tcgaggatct ttgagagcca tcgccatcct gcaggagatg actgatcaat
    17401 tcccgcaacc tgatctcatg tctgcgaacc aggcagcaaa tgtcgctcaa agcccgttcc
    17461 cgttcatctg ccgccatctc catgtcggcc tgcgccagtt ccagctgcca atgtgtgggt
    17521 tccacagaag tcctcatttg tctgtcactg tcctgatgag acagccggcc gatccacggc
    17581 ggagctctca ggagtgctgc gtggcaggcc tgcggatact cgacgcggtc catgagcggg
    17641 cctttcgtcg catatcctgc catcggatgc atggcaatgc aaaacactat caaatagtag
    17701 acaaggaatg gtcgaggtgt aacgtagatc agagacatgc gaatccttat caatgttgat
    17761 gggtgctgga gggatctgag gtgggagcac tggatatttc gccgatctgc cctgatgaca
    17821 gcggcccgct tccgtcagcg ttcctcctgg tcgcatcgat agccaatctg caggaaattg
    17881 agttgagttt cgggcttgcg gcgcggaagg aagctttcga gcgtctccgg ttggagatgc
    17941 accggattgc acgccgaccc ttcgtcacgg aactcgagtc ggcaggcgtc atcgaggctg
    18001 tcatcgaatg ggaggcaggg gaggggcgcc ccgaccatct gatccatcgc cttgcctgca
    18061 atatgctgtc gcatccgatc gtctcggaga gcggatgctt ctatgtcgtg ccggaaatcg
    18121 gcggcgcggc gctggaccgg gaacatggcg acatcgattc cgccaaggcc ggtgcctatc
    18181 ggcagctgga cagcagttct gtcaagatcc aacagccctg gcacctgttt cgctatcgcg
    18241 aagatatgag ccgggcagcg gagctgcttc agaaaatcga ccgtggcgaa gcgatgcttg
    18301 tctggcaggc gatcgtttcc cctgcagaca gggcgccctt gctgtatttc gagggattgc
    18361 tgcgcgtctc gggcgaggat ggggcgctgt tctcctgcga ggccaatatc agggctgccg
    18421 aacgcgtggg tctcgcccct gagcttgatc gccggctggt atctcgcgct cttgaccagc
    18481 tcgagacgga cgacgacgtc cgcatggggg tgaacatttc cgcgaggagc gcctcactcc
    18541 accgttatgg cagggatgcc ggctggcgtg agcttagatc acgtctggag cgcgagcccg
    18601 agctggcccg ccgcctggtg atcgagatca ccgagacggc cgacttcgcc tcccttgatg
    18661 aggccgctga atttgtatcg ttcatgcaac atctgggctg ccaggtggcc attgatgact
    18721 ttggcgccgg tcggggatcg atcatccaat atggagcgct atgcgctgat atcatcaaga
    18781 tcgatggcag cttcgtccag caggcgggcc aaaacgagaa gggccatgcg accttccggc
    18841 atcttgtcgg actggcgcgg gcgctggcgc cgcaggtcgt ggtggagggc gttgaaacgg
    18901 cggatcaggc agaattggcg cgcggaatcg ggatcgactg gttccagggc tatcatttcg
    18961 gacgcccctc gccgacgcgc gcgctcggcg tttcggccga tgcgcgagcc atcatggcgc
    19021 tgagaaattt ccaggatcgg tttcgcgcct tctcgaaggc cggcggtgcc ctggcatgat
    19081 gcgccgggtc atcctggaga agcggcggat atggcgggcc ttaggctgcg ctgagcgatt
    19141 gcccgcgcgc cccgcaagat cggaaggatg aagaccgcaa acccaatggc cagccaggcc
    19201 acgcgcccgc cacatgggac gtgttcgtgg tccttgctca tgacgaaagg atgagcctcg
    19261 atggcccggg ctatggcagg cccccttggc gatcttgcga cgggatgagc ctggactgct
    19321 tggaaaagca tcaggaacgc tttgtttgaa aaacagaaaa tgacagaaga aaaacggagg
    19381 gtggcatgac cgaacggcaa ttggggccaa taagaaacat aggtagatca tgcagaacac
    19441 caacacgcaa tgcggcgacc ttggagatga tggagactca ttaaacgctg taatgctggc
    19501 ggagcgatgg tcacaggccg ccgccgcaat ggccaggctg gcggccgcgc gggatctggg
    19561 ggatatcgtg gcaatcctgc ggcaatcggc ccgcgccgcg gcgaatgccg atggcattgc
    19621 gatcgtgctg cgtgagggcg atgcatgtca ttatcttgcg gaagatgcga tggagccgct
    19681 gtggcaaggt cagcgctttc cgcaagatac gtgcgtttcg ggctgggcga tgcagaacag
    19741 gacatccgcc gccatcgccg atatcaccct tgatccgcgc gtgccgcagg atgcctaccg
    19801 gacgaccttc gtcaagagca tggcgatggt tcccatcggg gatggcgagc ctgtcgcggc
    19861 cctgggcgct tattgggctt caatcggcca tcccgatgcc catgaaatgg ccgttctgga
    19921 ggcgctggcg caggcagcct cgacagcgct ggcgcatggg cgcctggtcg ctgaaatggc
    19981 agagctcaat cgcgatcttg aagcacgcat cgaggagcgg accaaggaac tggaagctgc
    20041 gcatcaaagc ctcatgcaga ctcagaagct cgagctgatg gggcaattga ccgggaatgt
    20101 cgcccacgat ttcaataacc tgctttcacc gatcatgacg agcctggatc ttatcctcca
    20161 gacagggtct cccggacagg ctgccctcgc gccggcccag gtggcgatgg aagctgtcga
    20221 acgcgcccgt gcgcttgtcc agaggctgct ggcattcgcg cggcggcgcc cgctcgcgca
    20281 tgccgtcatg gacatcaggt cgctcctcaa gggaatgcag tcgctgctcg agagcaccgt
    20341 tggcggacgg atccagctcc atattgctgc accgcccggc ctgccccatg tcgcgggtga
    20401 ccggcagcaa ctggaaatcg cgatcctcaa ccttgtggtc aacgcgcgcg acgccatgcc
    20461 cgatggtggc gttctcgcca tctccgcaag ctatccatcg gccccggaag gccatttcgc
    20521 caaggatgac ggctttgtcc ggctggtcgt agccgatcag ggcgagggca tggacgaggc
    20581 gaccaggacg atggcgctcg agcccttctt cacgaccaag ggcgacggct cgggca
//
