LOCUS       NZ_CP109848            20237 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Paenibacillus polymyxa strain K16 chromosome.
ACCESSION   NZ_CP109848
VERSION     NZ_CP109848.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 5978518)
  AUTHORS   Manfredini,A., Vitali,F., Sas,L., Trzcinski,P. and Canfora,L.
  TITLE     Genome sequence of the Paenibacillus polymyxa K16 strain isolated
            from soil of tomato roots
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5978518)
  AUTHORS   Manfredini,A., Vitali,F., Sas,L., Trzcinski,P. and Canfora,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2022) Research Centre for Agriculture and
            Environment, CREA - Council for Agricultural Research and Analysis
            of the Agricultural Economy, Via della Navicella 2/4, Rome 00184,
            Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: SPAdes v. 3.14.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 270.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 06:01:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,334
            CDSs (total)                      :: 5,275
            Genes (coding)                    :: 5,199
            CDSs (with protein)               :: 5,199
            Genes (RNA)                       :: 59
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 52 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 22 of 76
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:36:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5607402
            Orig. end                         :: 5627639
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109848.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Strain stored in SYMBIOBANK
            culture collection of the National Institute of Horticultural
            Research in Skierniewice (PL).
FEATURES             Location/Qualifiers
     region          1..20237
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="proteusin"
                     /region_number="16"
                     /rules="(PoyD or TIGR03793)"
                     /tool="antismash"
     cand_cluster    1..20237
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PoyD or TIGR03793)"
                     /kind="single"
                     /product="proteusin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20237
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10237](+)"
                     /cutoff="20000"
                     /detection_rule="(PoyD or TIGR03793)"
                     /neighbourhood="10000"
                     /product="proteusin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10237
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PoyD or TIGR03793)"
                     /neighbourhood="10000"
                     /product="proteusin"
                     /protocluster_number="1"
     gene            complement(893..1357)
                     /locus_tag="OF859_RS25020"
     CDS             complement(893..1357)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370208.1"
                     /locus_tag="OF859_RS25020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="copper amine oxidase N-terminal domain-containing
                     protein"
                     /protein_id="WP_053324795.1"
                     /transl_table=11
                     /translation="MLKKAVNTLLFSLIFFLTVDQANAALPKYLYNLVIKVNNVEVTSD
                     ANPYIERATNTTYVPIRFVSENLGETVNWNREKQEVTIRSEDGKTVRLTIGSKVAYVNE
                     QQKILPSAPVMPVSPNRVMVPLRFVSEVMGATVDSSKKDETLYVNITKAN"
     misc_feature    1394..1412
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 24.33"
                     /tool="antismash"
     gene            complement(1696..1935)
                     /locus_tag="OF859_RS25025"
     CDS             complement(1696..1935)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="OF859_RS25025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_053324794.1"
                     /transl_table=11
                     /translation="MSEFYYYVEEKKIALSILLMTISIPSLAANAQGQEPSSNEFNQYI
                     KDAIEQEIQVNEGDDNGYKVFYYAGKSDEPYINY"
     misc_feature    complement(2076..2092)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.87"
                     /tool="antismash"
     misc_feature    complement(2077..2090)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.47"
                     /tool="antismash"
     gene            complement(2204..2389)
                     /locus_tag="OF859_RS25030"
     CDS             complement(2204..2389)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280170.1"
                     /locus_tag="OF859_RS25030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="WP_025723187.1"
                     /transl_table=11
                     /translation="MNRYETVTLIQQMEHARQHLHDLYEEYGFGHACVLEQSMLLDELI
                     NQYNRMFQTKKLPHYV"
     misc_feature    2406..2420
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.01"
                     /tool="antismash"
     gene            complement(2612..3997)
                     /locus_tag="OF859_RS25035"
     CDS             complement(2612..3997)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369730.1"
                     /locus_tag="OF859_RS25035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_103744461.1"
                     /transl_table=11
                     /translation="MEITPTIQAEIQTYLKRKGLTMTKFGHTIDLNVGTVSSIVTGNRS
                     ISVHQLDSITAGMNLPPDYFYERYIEECIEESPLNWKKISPFLYRCIELGRLNCLQRVV
                     ILLLDNPNYPPSLFEVAENVYKDGYNEAAAYLYKKVAESEKQQHSERLAVCQYRLFQIN
                     IGQDRAVNLQAAIEFAPFVDRLDEIEQLDALKDLANVYRSLSMWDMVYEFSSQMGQMGK
                     IQYNLVHNSKRKGTEPRKKLSRPLFVYIVYSELLCANACDAKGDHEQALEHIRGYADLS
                     WVKEKDPDSLYWKKQYQQWAKINTYVNRLMSGDISVLPDYVEHMAAEKEILAELLNVLE
                     VANRYNVDVDHILQRFEPQIAAHQEPSSSDMYTQQVLPGEYIRFWYKMAKYNLNKGRYP
                     YGFKCLLNAWEKAESINKGILSHNCAMLFERFKAYAVPETRDQLQSLWEKIDQKDGFSF
                     RNN"
     gene            4196..4642
                     /locus_tag="OF859_RS25040"
     CDS             4196..4642
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280167.1"
                     /locus_tag="OF859_RS25040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_264454084.1"
                     /transl_table=11
                     /translation="MKNTGMTRPLDTLGRIVIPKELRTSMGIQAGESLEFYLDVETGLL
                     SMWKYIGVCCHLCYSVQDLSYFRGSFLCKKCINDLKGNIGVSQIPVPVVKEPYKEKRAH
                     RSPSQHLVEKLRRLMREHPAAKQGEYAEWLGVSQGRISQLKKLL"
     gene            4857..5315
                     /locus_tag="OF859_RS25045"
     CDS             4857..5315
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016819479.1"
                     /locus_tag="OF859_RS25045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_071559092.1"
                     /transl_table=11
                     /translation="MTTTRKKKKSSQEQSASVCTCINLRRSSMAVTGLYDRYLAPSGLN
                     ISQFSLLKHLTALGPASVSELALEMRLDRTTIVRNLKALEHSGYVEDTSAEGSRNRCLT
                     LTESGRTVYNDAAELWEEAQRFLQESLGIGQLQILNTLLSKIENLDGY"
     gene            5575..6453
                     /locus_tag="OF859_RS25050"
     CDS             5575..6453
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369171.1"
                     /locus_tag="OF859_RS25050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(Fe-S)-binding protein"
                     /protein_id="WP_081365945.1"
                     /transl_table=11
                     /translation="MVESVPDMRENIAAEMKTYVSEDKGNVCEELQSPYYEEPIIQFAA
                     ANDPLFEEYKRVVGPDHATPQEAFERSFGTGSFDGGTVISVVLPISETIRKANRAQKER
                     ASREWALLRTFGDEYFVQSARRHLAGYLTGLGYRAVAPLDTDWYSIYGATGGPISNWSE
                     RHIAYAAGLGTFSINDGFITEKGIAIRLLSVVTDLKVPPDARASKVTGHTGNCLLCSKG
                     ICGVCITRCPVQAISKEGGHDKIACMKFVYGQASRDFAVLNGGEAKSGAGCGLCQTKVP
                     CESRNPMRTAR"
     gene            complement(6510..7022)
                     /locus_tag="OF859_RS25055"
     CDS             complement(6510..7022)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429111.1"
                     /locus_tag="OF859_RS25055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_025723192.1"
                     /transl_table=11
                     /translation="MKKQWKLTAAILYGGVMMYGTAGAAGASGTEPSNSLQTLTPNDSI
                     QGAARQWNAEPQDDHGHGHVHRHRHGKRPGGLNHHLNEKVAKLLGITPVQLEKELGQGK
                     SLAEVAKSKGIQEDQLIDKLKNEMTVDLKRLVNRKGPITFDRKSGASNEAQLKHEAGTP
                     STSTNEN"
     misc_feature    complement(7216..7234)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.12"
                     /tool="antismash"
     misc_feature    complement(7287..7298)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.09"
                     /tool="antismash"
     gene            <7414..7656
                     /locus_tag="OF859_RS25060"
                     /pseudo=""
     CDS             <7414..7656
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010347573.1"
                     /locus_tag="OF859_RS25060"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="SKRTSKTISISEHFKKWEDMDKMYEVLLTVEGYQSSRSANVTSNT
                     ITIGGNNGTRNQEIELVVTADDGTWDDYIDYLEID"
     gene            7739..8812
                     /locus_tag="OF859_RS25065"
     CDS             7739..8812
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369725.1"
                     /locus_tag="OF859_RS25065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_071559093.1"
                     /transl_table=11
                     /translation="MFIAKAGKWTAIMSITLSLFTGSLVISSNQALAADCSSGYVALTF
                     DDGPNPSNTKALLSALKQNGLRATLFNLGQNVQNNPSLVRDQQAAGMWIGNHSWSHPHM
                     TQLNTSQMSSEITRTQQTIQSITGTVPKLFRPPYGETNATLKSIETQNGLTEVLWNVDS
                     QDWNGASTAQIVAAAGKLKNGDVILMHDQYQTTLQAIPQIAQNLKNRNLCSGMISPSNG
                     QAVAPDGGNGETPPNKGTKVEAENMTKGGQYTSNISSPFNGVALYSNNDSVKFTQNFTS
                     GTHSFSLRGASNNSNMAKVDLKIGGQTKGTFYFGGSSPAVYTLNNVSHGTGNQEIELVV
                     TADDGTWDAYIDYLEII"
     misc_feature    complement(8816..8825)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    8881..8895
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.42"
                     /tool="antismash"
     misc_feature    complement(8906..8918)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 21.58"
                     /tool="antismash"
     gene            8990..9925
                     /locus_tag="OF859_RS25070"
     CDS             8990..9925
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429108.1"
                     /locus_tag="OF859_RS25070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_023987330.1"
                     /transl_table=11
                     /translation="MEWYFAYKDELEKVFRQAEERTSTFPAPFQQEGLDYLARFNPLRE
                     DSTRNYICYLLPFWLQELTGLPPETCRKLSLGNVFVMAHYLIQDDVMDTAADNRKTQLA
                     LSQLFYTECLSVYRDLFPATSPFWSYARTYIDEWAVSVISEGTEDYFQGERNKVALKAS
                     PLKMAGTGALLLAGRADLIQIITDMTDLALLVLQMSDDWADWSEDLVEHSYNCLLSHIS
                     AEQKTAYCEGLGPKQIQEAIYVRGALANYVNIADQAVQQLETLEPSIHGLRSFAHSIAA
                     ELGEVAAEIEGGRSHLRRGGLDYWLSKNMK"
     gene            10001..10237
                     /locus_tag="OF859_RS25075"
     CDS             10001..10237
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     proteusin: TIGR03793"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429107.1"
                     /locus_tag="OF859_RS25075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="WP_013309068.1"
                     /sec_met_domain="TIGR03793 (E-value: 9e-19, bitscore: 65.3,
                     seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATEVLQTQVIQKAWEDASFREKLMTDPKSAIRDVLGVVIPDHIQ
                     IKTLEETPDQFFLVIPPDPSGVLAASQKPRSMW"
     gene            complement(10337..12226)
                     /locus_tag="OF859_RS25080"
     CDS             complement(10337..12226)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686330.1"
                     /locus_tag="OF859_RS25080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_023987329.1"
                     /transl_table=11
                     /translation="MRHVPKAVATLLAVIVLLLSLGHSAYAAKMTPERDYKIPSWDMRW
                     GAVDEDGSLDEVKDRSKIWMHIEDDTQLLRQPGNIGSAWIRIKLPSLNDETPAVLFENI
                     YGRHITLYKDGTNFYESYRGYNYENNRILIPLKPEDSGKTLYIWTESSKNRLGIIGNVM
                     TGDYRDLLGTLVRMDVLDIVLGSTFIFIALVLLICSFFLFRPSMAMWLSLSAIVLSIGL
                     MIVTYSPFLYTFYRGYGKLYLQLFDVALFILLPSLAYFFEQNINSIILIRKFRKVLTAY
                     SLFCFTMMIVNLGAQERLNDIYYFFSVVILGILLVALMILLVFASVRMALKGDHEAIIL
                     STGFAFFAVISIGELSWFFILDGHYNMFLWKWGVFGFVLALIAILGRRLAEKHKQVVRY
                     SRELEMFNNELQRSEKMEIISDLAASVAHEVRNPLQVTRGFLQLMSKQEDGKNKDYLHI
                     ALEELDRASAIITDFLTFAKPEFDQTKTLTILDEFKHIEGIISPMANLQGGKISLDIPQ
                     EIKVRGNSSKFKQAFINIIKNSIEALRDQGHIHIWAYVDNGEAVIHIRDNGEGMDAQTL
                     SRLGEPYFSNKTKGTGLGMMVTFRIIETMNGKITFTSAKGVGTEATIRFPVAG"
     gene            complement(12359..12646)
                     /locus_tag="OF859_RS25085"
     CDS             complement(12359..12646)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369721.1"
                     /locus_tag="OF859_RS25085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_023987328.1"
                     /transl_table=11
                     /translation="MEWYMFGPMISRIRVGQKASTPGFSRTLIRRPEGLYWTDGGQAGK
                     IVEIRDYLFSDIWTIYEDEDCEPWLGLREQKERREQEMIINQYEDFTKNE"
     gene            12830..13090
                     /locus_tag="OF859_RS25090"
     CDS             12830..13090
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369720.1"
                     /locus_tag="OF859_RS25090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_069079713.1"
                     /transl_table=11
                     /translation="MVNRDDRTTELHDNDFEPSLQNAETNWENLTEGPDTPLEDVPDAD
                     ELTPNSPVDPSAPAEFLHGTDLLNGTDGDEEEQLDSDDMLD"
     misc_feature    complement(12865..12879)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.27"
                     /tool="antismash"
     gene            complement(13451..14122)
                     /locus_tag="OF859_RS25095"
     CDS             complement(13451..14122)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595536.1"
                     /locus_tag="OF859_RS25095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028543187.1"
                     /transl_table=11
                     /translation="MEYHRITHIEDPLFASMHQLMQEVFPPEEVLEYSLWKEPLEDPGI
                     RVFVAVQDDKVVGATEYRYYEDFNVAMTDFTIIGQPGLGIGRFLWTRRQNDLHALAQAS
                     GQQLQGMFAEIYDPYRGNEHPFGGVKPMDPYVRREVLSHLGYKRLDLDYVHPSWNNDGV
                     AVEGLNLGFMPEDDSVEEVSAKLVAAFIRRYYTVLPNKPAVWNEMVDGLEAKSTVALLP
                     L"
     gene            complement(14147..14818)
                     /locus_tag="OF859_RS25100"
     CDS             complement(14147..14818)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280151.1"
                     /locus_tag="OF859_RS25100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_023987324.1"
                     /transl_table=11
                     /translation="MYRKEWFIFDGDRPLRAVIRNYSTADYEGLLRIQQESFPPPYPQE
                     LLWSREQIASQTEHFADGALCVEIEGELAGSVTSLLMHYNPEHPQHTWEEVADDGYIRT
                     HCEDGKALYVIDLAVRPAYRRLGLGKWLIFSLYHLVIERRLDRLLGAGRMPSYQHHADQ
                     LSAEAYVDAVVSGELKDPVLTFLLRCGRVPVCILPNYLDDEQSRNYAALMEWRNPFHTH
                     H"
     gene            complement(14835..15695)
                     /locus_tag="OF859_RS25105"
     CDS             complement(14835..15695)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280150.1"
                     /locus_tag="OF859_RS25105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="WP_028543188.1"
                     /transl_table=11
                     /translation="MSKIRVSAVQYHLHTIDSFEQFALQSEHYIKTAEEFGAEFVLFPE
                     FFTTQLMSIGDSEGKALTINYLPDFTDRYLELFTSFARRTGMHIIGGTHVVRRNERLYN
                     TAHLFYPDGRVATQDKIHITPTEVHEWNMAPGEGLNVFETSKGTIAMLTCYDIEFPEIV
                     RMAKAKGADIIFCPSCTDDRHGFYRVRYTSHARAVENQVYVVLTGTVGALPTVDFMRAN
                     YGQAAVITPNDIPFPPRGIMVEGELNNDMIVTADLDLQLLYDVRERGSVTTWRDRRTDL
                     YTDWS"
     misc_binding    15857..16128
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
     gene            16219..17301
                     /locus_tag="OF859_RS25110"
     CDS             16219..17301
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369718.1"
                     /locus_tag="OF859_RS25110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="WP_013309063.1"
                     /transl_table=11
                     /translation="MSTNENSLELLREQLDATNLQLLELLSQRAELAGQLGKLKEAQGV
                     PDFDPVREQQMLDKLIAANRGPFDDATIRQLFKNIFKASLNYQKEEHKKHLIVSRKNQP
                     DSTVIKVKDTLVGGKASIMVAGPCSVESYQQTREVGAALKEAGVPILRGGAFKPRTSPY
                     DFQGLGVEGLQILKRVGDEFGLATISEIVDPRHLEEAIQYIDIIQIGARNMHNFELLKA
                     AGETRTPVLLKRGLAATMEEFLHAAEYIVSRGNTQVMLIERGIRTYEKWTRNTLDISAV
                     PILKKESHLPVLVDVTHSTGRKDILAPCAKAALAAGADGIMVEVHPNPAVALSDAQQQL
                     NIPEFHNFLSEVKESGLFKA"
     misc_feature    complement(16221..16236)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 22.59"
                     /tool="antismash"
     misc_feature    complement(17498..17507)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            17538..19160
                     /locus_tag="OF859_RS25115"
     CDS             17538..19160
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429103.1"
                     /locus_tag="OF859_RS25115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1593 domain-containing protein"
                     /protein_id="WP_071559094.1"
                     /transl_table=11
                     /translation="MRERKGLSIFLLAMICILIISGCSSKAPAVVKDESAAPTEKQTEN
                     KVENKATKARTVITTDGEVDDMNSVIRFLLYSNEMDLSGIVLTSSVYHYAGDEKAGIKP
                     FRWTGTQWVTDMIDAYGEIYPNLIKHAEGYPKPEYIKSVTKIGNISNKGEMDKETEGSN
                     FLKTLFLDDDKRDLYVQTWGGTNTTARALKSIEDEYKNTDQWETIRKKVSDKLVLYIIL
                     DQDDSYNNYIAKNWPDIRIINDQSNFWHFAYAWKMHTEEVNSKLHGEWNFKNIKDGHGK
                     LLNMYALMGDGNIIKGELDEEQRGSEAYLKKNPQYDKYDFISEGDSPSFFYLIDNGLRS
                     MENPTYGGWGGRFGVVNDKLFRNTVLDYDVHTKKFEAEYSLMRWFDDIQNDFAARADWA
                     IASDYKDANHNPTLTIKEGLDLTASPGEKITLHAEGSDPDGNQLTYKWWRYFEADTYED
                     SKVKPAQVKPELLGEMQLGLHREVAKGEKVNTIDLQGSDTNAVSFTVPADAKSGNTLHI
                     IAEVQDNGKHPLKHYQRVIVTVK"
     gene            complement(19247..20107)
                     /locus_tag="OF859_RS25120"
     CDS             complement(19247..20107)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369717.1"
                     /locus_tag="OF859_RS25120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TrmB family transcriptional regulator"
                     /protein_id="WP_028543189.1"
                     /transl_table=11
                     /translation="MKPKPNILEILRDLNFSEYEAKAYVTLLESSPLSGYAVSLNSGVP
                     RSKIYEVLSGMVSRGDIMVSQENTPLYVPLPPHELIAQRKRKAEQIFNVAQESLEQYTA
                     SFQNRENIWNISGYEAIINRINEGVKGAKHRILLEIWKEDAEVFRDALEQVAQQGIEVI
                     IVAYGELNFDFATVYRHDMSEEITSEIGGRWIVLSVDDREVVAGILSLGDDSRAAWTLH
                     PGLVMPITEVIIHDIYIMEILYEFREQLEAKFGPNLIHLRNKFAMGPNGKGYYVPLTEK
                     SIKRV"
ORIGIN
        1 tatgctttgc tgttacaatg gtcttactat gacatgtctg catggagcac tgggttacca
       61 tcattttggc agactctgct ggattatcgc acctacaact ttgtcatgta ctttttctat
      121 tttatgctgg gcgcagtatg tgcttacatg acagacacct ggcgtggact ggccaagcag
      181 acgttgccgt ggaatgtatt tgtctttatt ggtttgttca tgctcatggg tcacacgatg
      241 ctgatccagt cgggcgatac gatcaatctt aatatttcca cctatttgaa gccaagcacc
      301 tttgttctaa tcgtgtcgca gttgctttta ttgtacggac tgctgctcca tttgcaaaaa
      361 gatacgcgat ccgagccgtt tcgccgtatg ctgaactgga tcggacgtta ttcttttgga
      421 ggatatctgg cccatgcgct cgtactggcc ttcatatcct actacacccg cccgcttgca
      481 ctgggcgatc atcattttac agctacgctc attacgttta tcgtcgtggc aagtgtttct
      541 ctgggcatca gctggctctt tgcccatctg cctggcggca actggattgt tggctctaaa
      601 ggcagacagc ggttgaactg gcactcgttt cgtttccagt tcctttcctc caaacgtgct
      661 tccaaaagca cgcaggagct tggctaatag aaaaaagatg ctacagcact aaaaaaggac
      721 actcccgatt cattccggga atgtcctgct ttaaattaaa ttaaaaacaa ttatcatatt
      781 acctaatgct tttatacaac aaggattcca gactaatgcg tttaccattt gatattattg
      841 aggcctcaac tagaaacatc ctatctatca actcaataag aagacggaat gattaattag
      901 ccttggtgat atttacgtac agagtctcat ctttcttact actgtctaca gtagcaccca
      961 taacttcgga aacaaatctg agtggcacca tgacacggtt aggagatacc ggcattacag
     1021 gagcgctagg cagtattttc tgctgttcgt tcacataagc aactttgctt ccaattgtca
     1081 agcgcactgt cttaccatct tccgaacgga ttgtaacctc ttgcttttct ctgttccagt
     1141 ttacagtttc acctagattt tctgatacaa atctaatggg aacataagta gtattcgtcg
     1201 ctctttcaat ataaggatta gcatcggatg taacctcaac attgttgact ttaatcacaa
     1261 gattatacaa gtatttagga agagctgcat tggcctgatc tactgtcaaa aagaagatta
     1321 aggaaaaaag taatgtgtta actgcttttt ttaacataac gcatcaatcc ttttctaatc
     1381 gaattacatt cattgtttgt aaaaagtata cttctctaca tgatttgaat ccttgacata
     1441 atcaattcgt ccaccatgat tccaaacatc atcactcaaa ttggtaacat tttcggggta
     1501 cttggttcca gcactattat taattaggaa tctcatattg ttccaaatag ctggcgagtt
     1561 aaatccgtat gaaaaggatg tattagggga accagcatta ttcatggcaa tagcagtaac
     1621 tcttttaacc gatctaccag tcgtacccaa accattaaat ttgactacat agatattaga
     1681 atagttaatt tgtctctaat agttaatgta aggttcatcc gatttgccag cataataaaa
     1741 tactttgtaa ccattgtcat ctccctcatt cacttgaatt tcttgctcaa tagcatcttt
     1801 aatgtactgg ttaaattcat tcgacgaagg ttcttgtcct tgagcatttg cggccaagct
     1861 cggaatggaa atggtcatga gtagaataga aagagcaatc ttcttttctt caacataata
     1921 ataaaactca ctcatgtata cctgccataa aagtctctta atatgtgtat attatcctca
     1981 aactgcaatg ctcgcttatt gagctctgct ttttttattg ccttagtatg ccgtcccctc
     2041 aaaaaagtga ttaacatttt atgacatgaa tatgtatttt attttaaaac aagtcattat
     2101 atagaataat aataaacatt acgcgggaaa agtcacagtt ttgcataaca aagaagggag
     2161 aggatgtact gaacataaca tcgtcgttgt taatgatcta gccctaaaca tagtgaggca
     2221 gcttcttggt ttgaaacata cggttgtact gattaatcag ttcatccaaa agcatagact
     2281 gctcaagtac acaagcatgt ccgaagccat actcttcgta aagatcatgc aaatgttgtc
     2341 tggcgtgttc catctgttga attaacgtta ccgtttcata cctgttcatc ctgcatacct
     2401 cctaatgaat ctcattttaa ggagggatgt acaaatacgt catgatcttt tttgaaccaa
     2461 aaaattacaa aataatacaa aaatagacca gcaataagct ggtccatagt agttacactc
     2521 gaattaagca ccgccgatga ttccagtggt agaaatcgtt ccatgacctt gattcggacc
     2581 ttggattggt actgcgagta ccaataagaa gctagttatt gcgaaaagaa aaaccatctt
     2641 tttggtcaat tttctcccat agactttgca actgatctcg ggtttcagga acggcatacg
     2701 ctttaaatcg ttcaaataac attgcacaat tatgcgaaag tattccttta ttaatggatt
     2761 cggccttttc ccatgcattt aggagacatt tgaaaccata tggatatcta cctttattaa
     2821 gattgtattt agccatcttg taccagaacc gaatgtattc tcccggtaaa acttgttgtg
     2881 tgtacatgtc agaagatgac ggttcctgat gagctgcaat ttggggttca aagcgctgta
     2941 agatatggtc cacatctaca ttgtatcgat tagctacttc aaggacgttc agtagctcgg
     3001 ccaaaatctc tttttcggca gccatatgtt ctacgtaatc ggggagaaca ctaatatccc
     3061 cggacataag cctgttgacg tacgtattaa ttttcgccca ttgttgatat tgcttcttcc
     3121 aataaagtga atcaggatcc ttctccttca cccaacttag atcggcataa cctcgaatat
     3181 gttctaatgc ctgttcatga tcaccttttg catcgcaagc attagcacac agcaactcgg
     3241 agtaaactat gtatacaaac agtggcctgc ttagcttttt tcgtggctct gttccttttc
     3301 gcttagagtt atgaaccaaa ttatattgga tctttcccat ttgacccatt tgagatgaaa
     3361 attcatacac catgtcccac atacttaatg acctatacac gttcgctaaa tcttttaatg
     3421 catctaattg ctctatctcg tctaaacgat caacaaaagg ggcaaattca atggcagcct
     3481 gaagatttac agctctatct tgtccaatat tgatttggaa caaacgatac tggcagaccg
     3541 ccaatcgctc tgaatgctgc tgcttctcac tttcagccac ctttttgtaa aggtaagccg
     3601 ctgcttcatt ataaccgtct ttgtatacat tttcggcgac ttcaaagagc gatggcggat
     3661 aattagggtt atccaataac agaattacaa ctcgctgcaa gcaattcagt cgtcctagct
     3721 caatgcatcg gtataggaaa gggctgattt ttttccagtt gagcggagat tcttcaatac
     3781 attcttcaat ataacgctcg taaaaataat ccggtggtaa attcattccc gcagtaatac
     3841 tatccagttg gtgaacagaa atagatcggt taccggtcac aatgctacta actgtaccta
     3901 cattcaaatc aatcgtgtga ccaaacttgg tcatcgttaa gccttttcgt ttcaaatagg
     3961 tttgaatttc tgcctgtatc gtaggtgtga tttccatgga taaaccaccc tttcagcatt
     4021 ttcatagaat ttcaatttca ataaacaata tagtttggaa actaatagta taattattga
     4081 ttttccttca cgataatacc atcttttgga acaaacataa atgcttttac tttattgatt
     4141 tttatatttt tattaggaga tcattggatg ttggacaatg gaaggagaag ctcatttgaa
     4201 aaatacaggt atgacacgtc ccttggatac gttaggccgg attgttatcc ctaaggagtt
     4261 gcgaacctct atgggtattc aagctggtga gtctttagaa ttttatttag atgttgaaac
     4321 aggattattg agtatgtgga agtatattgg agtctgctgt cacttatgtt attcggttca
     4381 ggatttaagc tatttcaggg gttcattttt atgtaaaaaa tgcataaacg atctgaaggg
     4441 aaacataggt gtaagtcaaa tacctgttcc tgttgttaag gaaccataca aagaaaaacg
     4501 agcacatcgg tcgccttcac aacatttggt tgaaaagcta agaagactta tgcgtgagca
     4561 tccagcagcc aagcaaggtg aatatgctga atggcttggc gtatcccaag gccgtatttc
     4621 ccagcttaaa aagctattat aggtaccgca cgactcatat tcttataatg ctaaataacc
     4681 agtcgttggt tgattttatg cgactggtta tttaaacttt tcattattag gaaggactac
     4741 aggagataaa tcatgataga ccgacagagc tgactgctgt gacaaatccc tctgacaaaa
     4801 cctgttattt ttgagatagt gtagatacac gaaacagtac aaagaatggg gaacttatga
     4861 caacgacaag gaagaaaaag aaatcttcgc aggagcaatc agcctcagtc tgtacctgca
     4921 ttaatctgcg ccgttcctcg atggctgtta cgggactgta tgaccggtat ctggccccga
     4981 gcggactcaa tattagccaa ttctcgctgc tcaaacactt aactgcgctg ggaccggcaa
     5041 gtgtaagcga attggcgctg gagatgcggc tcgaccggac aacgattgtg cgcaatttga
     5101 aagcgttgga gcatagcgga tatgtggagg atacatctgc tgagggcagt cgcaaccgct
     5161 gccttactct gacggagagt ggaagaacgg tatacaacga cgcagcagag ctttgggagg
     5221 aggcgcaacg ttttttgcag gagtcattgg gaatcggaca gttacagata ctcaacacac
     5281 tcttatccaa gattgaaaat ttggatggat attagacggc tcctcatcca ggtggagttg
     5341 tgttcaattc gtgtatatac acgaaaaaga tcgaaatgtt tattgcggta tcaaggctag
     5401 agatgtcttt aaaatgctat tatagcgatc gcattgcatt tgatcaccac tgcgggtcat
     5461 cggagaagta cttccagctg tagtaagagg tcagtgatat accttagccc gccacaagac
     5521 ttctgaaggg aagctttact gtgaacaata accacggaga gggatgtgga taggatggtt
     5581 gaatccgtac cggatatgag agaaaacatc gcagccgaga tgaaaaccta tgtgagcgaa
     5641 gacaaaggca atgtttgtga agaactgcag agtccttatt acgaggagcc gattatccaa
     5701 tttgccgcgg ctaatgatcc tttgtttgag gagtacaaaa gagtggtggg gcccgatcac
     5761 gccacgccgc aggaagcatt tgaacgctcc tttggaacgg ggagttttga tggaggcacc
     5821 gttatcagcg ttgtgctgcc gatcagcgag acgatccgta aggctaatcg tgcgcaaaaa
     5881 gaaagagcct caagggaatg ggcgctgctg cggacctttg gcgatgaata ttttgtacag
     5941 tctgcaaggc ggcatttggc gggatactta acaggccttg gttaccgtgc agttgcaccg
     6001 ctggatacgg attggtacag catctatggt gccacagggg ggccgatctc caactggtcg
     6061 gagcgccaca ttgcatatgc tgcgggtctt ggcaccttca gtatcaacga cggcttcatc
     6121 accgaaaaag gaattgccat ccgcctgctg tccgtggtga ccgatctcaa ggtgccgcct
     6181 gatgccagag catcaaaagt cacaggccat accggaaact gcctgctgtg tagcaagggc
     6241 atctgcggtg tgtgcattac ccgctgccct gtgcaagcca tcagcaagga aggcggacat
     6301 gacaagattg cctgcatgaa atttgtgtac ggccaagcat cacgggactt tgcggttcta
     6361 aacggcggcg aggccaaatc aggtgccggc tgcggactgt gccagaccaa ggtgccctgc
     6421 gagagcagga atccgatgcg gaccgcccgg tgaatatgga aaccttgagg accgccattc
     6481 tcaaggtttt ttagtgcaaa aagatacatt tagttttcgt tagtcgaggt tgagggcgtc
     6541 cctgcctcat gttttaattg agcttcattc gaagccccgg acttacgatc gaatgtaatc
     6601 gggcctttac ggttcacaag ccgtttcaaa tccacggtca tttcattctt caatttatca
     6661 atgagctggt cttcttggat gcctttgctt ttagcaacct cggccaatga cttcccttgt
     6721 cccagttctt tctctaattg gacaggagtg atgcccagca gcttggctac tttctcgttg
     6781 agatggtgat tcaagccgcc aggccgtttg ccgtgtctat gtctgtgaac atgcccatgc
     6841 ccgtggtcgt cctgtggttc agcattccat tggcgggctg ccccttggat agagtcgttg
     6901 ggagtgagag tttgcaacga attactcggt tccgtcccgg aagctccagc tgcacccgca
     6961 gtgccataca tcatcactcc tccatacaga atagcagcgg tcaatttcca ttgttttttc
     7021 atgttgcaga cccttctttc atccttttta aaataatatt ttcttttccc gctattttta
     7081 ttcatatttt cgcctgttaa gtaaccataa gacgggaaaa agaaattatg aacattgcta
     7141 tcacaattac acccaacctt cgatctacac gcgctccttg ggaaccctat cctgtagagt
     7201 gagcaagaat gaataactat actttctaca atataacttg aaatttttca agttatatat
     7261 taaggaaaat atgataaaat caaattgtaa acgtttacaa tttgattttt ggaaagacat
     7321 gttgcatcaa gctactacag aatgcactgc atcatagtga ggctcaggag gaaagtccaa
     7381 tgaaacaaaa aaataaggtt atttttatgg acatccaaac gcacgagcaa aaccatatcg
     7441 atcagcgagc acttcaaaaa gtgggaagac atggacaaga tgtatgaagt tttacttact
     7501 gtagagggat accaaagcag tcggagtgcc aatgtaacta gtaatacaat tacgattggc
     7561 ggcaataatg gcaccagaaa ccaagagatc gagctcgttg taacagccga tgacgggaca
     7621 tgggatgatt atattgacta tttggagatc gactaaaatt tcaaatctag taaagagttg
     7681 aatcggttcg gagtatcgga acatccatac tatattaaaa tagctaggag gtagatccat
     7741 gtttatagct aaagcaggaa aatggacggc gattatgtca atcaccttgt ctttattcac
     7801 aggctcgctg gtgatctcga gcaaccaggc gcttgcggct gattgctcga gcggttatgt
     7861 agcgttaacc ttcgacgatg gtcccaatcc ctctaacacc aaagcgctgc ttagtgcttt
     7921 gaagcaaaac ggtttacggg cgacgctctt taatcttgga caaaacgtgc agaataatcc
     7981 ttcgcttgta cgtgaccagc aagcagcagg catgtggatc ggtaaccatt catggtcaca
     8041 tccgcatatg acacaactga acacatcgca gatgtcatcg gaaattacac gcacgcagca
     8101 aacgatacag tccattactg gcacggtacc aaagttattc cgtcctccgt atggcgagac
     8161 caacgcaacc ctcaaatcca ttgagaccca aaacggtctt accgaagtat tgtggaacgt
     8221 tgactcccaa gactggaacg gcgcctccac cgcacagatc gtagctgctg cgggcaaact
     8281 taaaaacggc gatgtgatcc tgatgcatga tcagtaccag actacactcc aagctattcc
     8341 acaaattgca cagaacctca aaaaccgtaa cctttgctct ggtatgatct caccaagcaa
     8401 tggccaggcg gtagcgcctg atggcggaaa cggcgaaacg ccgccaaaca aaggcacaaa
     8461 agtggaagca gagaatatga ccaaaggcgg tcagtacact tcaaatatca gctcgccgtt
     8521 caacggagtg gctttatact ccaacaatga ttcagtgaaa ttcacacaga attttaccag
     8581 tggcacccat agtttttcac tccgtggtgc ttcaaataat tccaacatgg ccaaagttga
     8641 cttgaaaatt ggtggacaaa cgaagggtac cttctatttc ggcgggagca gtcctgcggt
     8701 ctatacttta aacaatgtca gccatggaac cggaaatcaa gagattgagc ttgttgtaac
     8761 agccgatgat gggacatggg atgcttatat tgactattta gagatcatct aattttaaaa
     8821 aaacaaaatc ccggcgggat cttcccagcc gggatttttg tcgatatcct ttttacaaca
     8881 ttctcaatgt aaaaataaca aaatcttgaa aattgcaatc tgttgtataa tggcggttaa
     8941 caactagaaa tcgctataaa aatattcata agagatgcag gtgatagcga tggaatggta
     9001 ttttgcatac aaagacgaat tggaaaaagt atttcgtcaa gcggaggaac ggacgtccac
     9061 gtttccagct ccctttcagc aagagggttt ggactatctg gcgcgtttta atccgttgag
     9121 ggaagacagc acgcgtaatt acatatgcta tttgcttcca ttttggctgc aagaactcac
     9181 tggactcccc cccgaaacat gccgcaagct gtcacttggc aacgtgttcg tcatggctca
     9241 ctatttaatt caagatgatg tgatggacac agcagcagac aaccgtaaaa cgcaactcgc
     9301 gttaagccag ctattctata ccgagtgtct aagcgtatat cgggaccttt ttccggcaac
     9361 ctctcctttt tggtcatatg cccgaacgta tattgatgaa tgggccgtta gtgtgatatc
     9421 cgaaggaaca gaggactatt ttcaagggga aagaaataag gtcgcgctga aagcaagccc
     9481 attaaaaatg gccggaacgg gtgctttact gcttgcaggc cgtgccgatc tgatccagat
     9541 cattacagat atgacggact tggcgctact cgtgcttcaa atgtcggatg attgggcgga
     9601 ttggtcagaa gatttggtcg aacatagcta taactgtctt ttgtcccata tcagtgccga
     9661 gcagaagacg gcttactgtg aaggattggg acccaaacag attcaggaag cgatttatgt
     9721 acgcggtgcg ctcgccaact atgtgaacat tgccgatcaa gcagtccaac agttagagac
     9781 cctggagcct tctatccacg gcctacgtag ctttgcacat tctatcgcag cggagctggg
     9841 cgaagtggcg gcggaaattg aaggcggacg ctcgcatttg agaagaggcg gacttgatta
     9901 ctggctctcc aaaaatatga aataatgcta gatttttccg agtctttcgt ggtatactga
     9961 ctaggtaaat ataaccaatt gaatgagagg gtgatttttg atggctactg aagttcttca
    10021 aacacaagtc attcaaaaag cttgggagga tgccagtttc agagagaaac tgatgacaga
    10081 tcccaaatct gcaattcgtg atgtgttagg agtcgtgatc cccgatcata tccaaatcaa
    10141 aacgttagag gaaacgcctg atcagttttt tctggtaatt cctccagacc cctccggtgt
    10201 actcgctgca tcccagaaac ctcgctcgat gtggtaaatt ggttgttaat gcttatttaa
    10261 tctatattcc ttaatctcat gtgggtgggc tcagcaagct tccctccgtt ttctccgaat
    10321 gcaaaaaagc cggatattat ccggctacgg ggaagcggat ggtcgcttcc gttcccaccc
    10381 cttttgcact tgtaaaagtt atttttccat tcatcgtttc aataatccga aaagtcacca
    10441 tcatccccaa acccgtgcct tttgtcttat tcgagaaata aggctcacct agtctggata
    10501 aagtctgagc atccatccct tcaccattat cccgaatatg aatgacagct tctccgttgt
    10561 ccacataagc ccatatatga atatgaccct ggtctcgcag cgcttcaatg ctgtttttga
    10621 taatattgat gaacgcctgc ttgaacttgg aggaattccc gcgtactttg atttcctggg
    10681 gaatatccag tgaaattttt ccaccttgca aattagccat tggactgatg attccttcaa
    10741 tatgtttgaa ttcgtccaat atggtcaacg ttttggtttg gtcaaactct ggcttggcaa
    10801 aagtcaaaaa atcagtaata atcgcggacg ctcgatccag ctcctccaga gcgatatgta
    10861 gatagtcttt gtttttacca tcctcctgct tgctcatcaa ctgaagaaat ccacgtgtaa
    10921 cctgaagggg attacgtacc tcatgagcta cagatgcagc cagatcgcta ataatctcca
    10981 ttttctccga acgttgaagc tcattattga acatctccag ttctctggaa taccgaacca
    11041 cctgcttgtg cttttctgcc agtcttctgc ccaaaatcgc gatcaacgcc agtacaaaac
    11101 cgaacacgcc ccatttccac aaaaacatat tgtagtgtcc gtctagaatg aagaaccagc
    11161 tcagttcccc gatagagatg actgcaaaaa atgcaaagcc tgtcgataaa atgatcgctt
    11221 cgtgatcacc cttaagtgcc atgcgaaccg aagcaaatac cagcagtatc attaaggcga
    11281 cgagtaatat acccaggatg actacactaa aaaaataata tatatcgttc aaccgctcct
    11341 gtgctcctaa attaacaatc atcatggtaa agcagaacaa agagtaggcg gttaacacct
    11401 ttctgaattt gcgaatcagt ataatcgaat tgatattttg ctcaaaaaag taggctaatg
    11461 aaggtagcag aataaatagt gccacatcaa aaagttgtaa gtacagcttg ccatagccgc
    11521 gatagaatgt atacaagaat ggtgaatacg ttacgatcat cagaccaata gacaatacaa
    11581 tcgcacttag ggacagccac atggccatac tcggacggaa cagaaaaaac gagcagatca
    11641 gtaataccag ggcgataaag ataaacgtac tgcccagtac aatatctaaa acatccattc
    11701 tgaccaaggt tcccaataaa tcacgataat caccggtcat cacattgccg ataatgccca
    11761 gtctattctt actgctttcc gtccatatgt ataatgtttt accgctgtct tccggcttca
    11821 gcggaatcag aatacgatta ttctcataat tgtacccacg gtaggattcg taaaaattgg
    11881 tcccatcttt atacagcgta atatgcctgc cataaatatt ttcaaaaagt accgctggcg
    11941 tttcatcatt taacgaaggt aacttaatac gaatccaggc ggagcctata ttaccgggct
    12001 gcctaagcag ttgtgtatca tcttcaatat gcatccagat cttgctgcga tcctttacct
    12061 catctaaaga gccatcctcg tctaccgctc cccatctcat atcccaggag ggtattttat
    12121 aatccctttc cggggtcatt ttggccgcat aggcggagtg gcccagcgac aacagcaaca
    12181 caatgaccgc tagcagggtg gcgacagctt tgggcacatg cctcatgtta agcacctcaa
    12241 agttacatag taggatttac aagtttgtat acgttttaac attttttacg tcctgaaatt
    12301 atattattct atacgatgat gtgcatacct tcttcataag aagggtatca tgttcttttt
    12361 actcattttt tgtaaaatct tcatattgat taatgatcat ttcctgttcg cgccgctctt
    12421 tctgctctct aagccccaac catggttcac aatcttcatc ttcatagatc gtccaaatat
    12481 cggaaaataa ataatcgcgt atttctacta tttttcccgc ttgtccgcca tctgtccagt
    12541 ataagccttc cgggcgccga atgagggtgc gggaaaatcc cggggtagat gccttttgac
    12601 ctactcggat acgactaatc atcggaccaa acatgtacca ttccaattca tatccctccc
    12661 tatcttcttt atcatatcac agcagttacc aaaaaaacgc taattgagaa tgggcatatc
    12721 cctattcgca tgattttaac ttcctacaga cacactaact caaggtttag ccgttcttct
    12781 gtttgggtaa ggtcggaata agcaacaaaa agtaaaggag ctgagcaaca tggtcaatcg
    12841 tgatgatcga actactgagc tacatgacaa tgattttgag ccttctctgc aaaatgcaga
    12901 aaccaactgg gagaatctta cggaagggcc ggatactcct ctggaggacg tcccagatgc
    12961 cgatgaactg acaccgaaca gtcctgtcga tccttctgct cctgctgaat tcctgcatgg
    13021 taccgatttg ctgaacggta cggatggaga cgaagaagag caactggaca gcgacgatat
    13081 gctggattaa ccacttcatt ttatcgggtg aaagaaaacg gttacccatt ctgctgtgcg
    13141 ggtaatgaag tgtataacag aaaggagttg actgatctcc tatgtataaa gatccgatgt
    13201 ctcaggccga tatggagtat aagcgctaca aaaccttgaa tcctgtggaa gcagacgaac
    13261 gtaggaccgc ggacaagaca actacagatg atatttcggc cgaagataac ccacgaggag
    13321 tcgcatctgc gaagcccgaa cccagggatc aaggaaaatg aaagggtcaa ttagagaatg
    13381 caaaaagggt gcaacctaag ccacgcagat gatacatggc ttgagtgcac cctttttgta
    13441 cttcatatgt ttacaatggc agcaatgcta ctgtcgattt agcttccaat ccatctacca
    13501 tttcgttcca gacagcaggc ttgttcggca atactgtata gtaccgacgt atgaaagctg
    13561 ccaccaattt ggcgctcact tcttccacgc tatcatcctc aggcataaag cctaaattca
    13621 gcccttctac tgctacacca tcgttattcc aggacggatg tacgtagtcc agatccaaac
    13681 gcttatagcc cagatgagat agcacctcac gtcgcacgta cgggtccatt ggcttgacac
    13741 caccaaaggg atgctcgttg ccacggtaag ggtcatagat ttcagcaaac atcccctgta
    13801 actgctgtcc gctggcctga gcaagcgcgt gcaagtcatt ctggcgtcgt gtccaaagaa
    13861 aacgtccaat gcccagtccc ggttgtccga tgatcgtaaa atctgtcata gctacattaa
    13921 agtcctcata ataacgatac tctgttgcac caactacctt atcatcctgt accgcaacaa
    13981 agacacgaat acccgggtct tccagcggtt ccttccacag actgtactcc agcacttcct
    14041 ctggtggaaa tacttcctgc attagctgat gcatgctcgc aaacaagggg tcttcaatat
    14101 gagtaatgcg atgatattcc ataggatagg acacgctcct ctacaattag tgatgggtat
    14161 gaaacggatt tcgccattcc atgagggcag cataattacg cgattgttca tcatccaaat
    14221 aattcggcag gatacaaaca ggcacccgtc cgcaacgaag cagaaaagtt aataccgggt
    14281 ccttcagctc accagacact acggcatcca catacgcttc tgcgctcaat tggtcggcat
    14341 gatgctgata actaggcata cgccccgcgc ccagcaaacg atccagtctc cgttcaatca
    14401 caagatgata taaggaaaaa atcagccatt tgcccagacc caatctgcgg taagcaggac
    14461 gtaccgccaa atcaatcaca tacagcgcct tgccgtcctc acagtgcgta cgaatataac
    14521 cgtcatctgc tacttcctcc catgtatgct ggggatgttc ggggttataa tgcatcagta
    14581 ggctggttac cgaacccgcc agttccccct caatttccac acacagcgct ccatctgcaa
    14641 aatgctctgt ctggctagca atctgctccc ggctccataa cagctcctgc ggataaggtg
    14701 gggggaagct ctcctgctga atacgcagca atccctcata atcggctgta gaataattac
    14761 gaatcaccgc ccgtagcggc cgatcgccgt caaagatgaa ccattctttg cgatacatgg
    14821 cttatttcct ttcattagct ccagtctgtg tataaatctg ttctacggtc acgccaggtt
    14881 gtaaccgagc cccgctcgcg cacatcgtac aacagctgca aatcgagatc tgctgtcaca
    14941 atcatatcgt tgttcagttc accctccacc ataatgccgc gtggcggaaa aggaatgtca
    15001 ttgggtgtaa tgactgcggc ttgtccataa ttggcacgca taaaatcaac cgtaggcaaa
    15061 gcgccgacag ttcccgttaa aacgacatac acctgatttt caaccgctct ggcgtggctt
    15121 gtatatcgta cccggtaaaa tccgtgacga tcgtccgtac aggaaggaca gaaaataata
    15181 tctgctccct tggccttcgc catgcgaacg atttcgggaa attcaatatc atagcaggtc
    15241 agcatcgcta tggtgccctt ggacgtctca aacacattca gcccctcacc gggagccata
    15301 ttccattcat gcacctctgt cggtgtaatg tgaattttgt cctgagtggc cacacgcccg
    15361 tccgggtaaa acaaatgtgc cgtattatat aaacgttcgt ttctcctaac tacatgagtg
    15421 ccaccgataa tatgcatccc ggttcgacgt gcgaacgaag tgaataattc aaggtaccgg
    15481 tccgtaaaat ccggcaggta gttgatcgtc agagctttgc cttcactatc accaatcgac
    15541 ataagctggg tcgtaaaaaa ctcgggaaac agaacaaatt ccgcgccaaa ttcctccgct
    15601 gttttgatat aatgctccga ttgcagggca aattgctcga atgagtcaat cgtatgcaga
    15661 tgatattgaa ccgccgatac tctgattttg ctcataattc ctcctcggac atcacgtctt
    15721 ttagattgca agggggtccc gttgctgttc acattataac cgattcaaaa cggtgcgaaa
    15781 actgaacctg ataatcaggg cttgccttct gcacctactt tgtttaaaat aaggtcaaat
    15841 cttttgaata ccatgaacaa agacagtgac ggagacaagt aaacgtctaa cggattccca
    15901 cagaaaggta cgaattgctg agagcgtacc gcaatcccgc gttgaaattc actcccgagt
    15961 cgagccttga accgacagaa ggattcattc ctttgtccgc agtagaggtt tccggaagcc
    16021 cccgttacgg gcatcaagct cagaagttac tgccgtcgac tgaacgacgt tgacgattcg
    16081 tatgtatact tctgtaatta gggtggtacc acggcattcg tcccttgcga cgagtgcctt
    16141 tttgtgttat tttctgcaag aaaagtctat gggctgacag aaaaaacaaa tttaaattcg
    16201 ggaaggatgt agtactgtat gtctacaaat gaaaattctt tggagctgct gcgtgaacag
    16261 ctcgatgcta caaatttgca attgctggag ttgctgtccc aacgcgctga actggcggga
    16321 caacttggaa aattgaagga agctcaaggg gtacctgact ttgatccggt acgtgagcaa
    16381 caaatgctgg acaagctgat cgcggctaac cgtgggcctt ttgacgatgc tacgattcgc
    16441 cagctgttca aaaatatttt caaggcttct ttgaactatc aaaaagaaga acacaaaaaa
    16501 catctgatcg tcagccgcaa gaatcaaccg gatagcacag ttatcaaggt taaagatacg
    16561 ctggtgggcg gcaaagcctc cattatggtc gcaggtccct gctcggtcga aagctaccag
    16621 caaactcgcg aagtaggtgc ggctctcaag gaagccggtg tgccgattct gcgtggtggt
    16681 gcgttcaaac cacgtacgtc cccgtatgat ttccaagggc tgggcgtgga aggactgcaa
    16741 attctcaaac gtgtaggcga cgagttcggg ctggctacaa tcagtgaaat cgtcgatcca
    16801 agacatttgg aggaagcgat ccaatacatc gacattattc aaattggtgc gcgcaacatg
    16861 cacaactttg aattgctcaa agctgcgggt gaaaccagaa ctccggttct gctcaaacgc
    16921 ggactggctg ccacaatgga agagttcctt catgctgctg aatacatcgt ttcccgtggt
    16981 aacacgcaag tcatgctgat tgaacgcggt atccgtacgt acgaaaaatg gacgcgtaac
    17041 acgctggata tttccgctgt tccgattttg aaaaaggaaa gccatctgcc tgtattggtg
    17101 gacgtgactc actccacagg acgtaaggac atcctggctc cttgcgccaa agctgccttg
    17161 gctgctggag ccgacggtat catggtggaa gtccatccaa acccagcagt cgctttgtcc
    17221 gatgctcagc aacagctgaa cattcctgag ttccacaact tcctgtctga agtcaaagaa
    17281 tccggtttat tcaaagcgta aaactaattt tatgccggga ttcataaacc gctttcattt
    17341 tctcggacaa tgatgtaaaa ttgaatcagg attgtgactg atcatgcagg caaaacctaa
    17401 atgtatgtat gctcgacttt tgttgaggca tatatatgtt taggtttttt ttatgcctaa
    17461 atcttgaata aaaagaatac ctacatggtt ataatcctaa aaaaacatca taggaacgtt
    17521 cagagagggg aacaaaaatg agagaaagaa aagggttaag cattttttta ttagccatga
    17581 tttgtatttt gatcatcagt ggctgtagta gtaaggctcc cgccgttgtg aaggatgaaa
    17641 gcgctgctcc aacagaaaaa caaacggaaa ataaagtaga aaataaagca accaaggcca
    17701 gaacggtaat cactaccgat ggagaagtag atgatatgaa ctctgtcatt cgctttcttc
    17761 tttactccaa tgaaatggat ttgtcgggga ttgtactgac tagctctgta tatcattatg
    17821 caggtgatga aaaggctggc atcaagccat ttagatggac cggaacacaa tgggtaacgg
    17881 acatgattga tgcctacgga gagatttatc ccaacttaat caaacatgcc gagggatatc
    17941 caaagcctga atatatcaag agcgttacca aaataggtaa catttcaaat aaaggtgaaa
    18001 tggacaaaga aacggaaggc tccaatttcc ttaaaacact tttcctggac gatgacaaaa
    18061 gagatttgta cgtacaaact tggggtggaa ccaataccac ggccagagct ttaaagtcca
    18121 ttgaagacga atataaaaat acagatcaat gggaaacgat tcgaaaaaaa gtaagtgata
    18181 aactcgtact gtacatcatt ctggaccagg acgatagcta caataattac atcgctaaaa
    18241 attggcctga catcagaatt attaatgatc aatctaactt ctggcatttc gcttatgctt
    18301 ggaaaatgca cacagaagaa gtaaacagca agttgcacgg ggaatggaac ttcaaaaata
    18361 ttaaagatgg tcatggcaag ttgctcaata tgtatgcctt gatgggcgat ggcaatatca
    18421 tcaaaggcga gttggatgaa gaacaaagag gcagtgaagc ctacttaaag aaaaatccac
    18481 aatacgacaa gtatgacttt atttctgaag gtgattctcc atccttcttc tatttaattg
    18541 ataacggatt gcggagtatg gaaaacccaa cctatggtgg ctggggaggt cgtttcggtg
    18601 tcgttaacga taaattattt agaaataccg tgttagatta tgatgtgcat acgaaaaaat
    18661 tcgaagcaga gtattctctg atgcgttggt ttgatgatat tcaaaatgac tttgctgctc
    18721 gtgcagattg ggctattgca tccgactaca aagatgccaa ccacaatcca acgttgacca
    18781 ttaaagaagg attagaccta accgctagtc caggggagaa aataaccctg catgcagaag
    18841 gttcagatcc cgacggtaat caattgacct ataaatggtg gagatatttt gaagcagaca
    18901 cgtatgaaga ttctaaagta aaaccagcac aagttaaacc tgaattgctg ggggaaatgc
    18961 agcttgggct tcatcgagaa gtagctaaag gcgagaaagt aaacacgatt gacttgcaag
    19021 gtagtgatac gaatgcagtt agtttcacag ttcctgcaga tgctaagtca gggaatacgc
    19081 ttcatatcat tgcagaagta caggacaatg gaaaacatcc tttaaagcat tatcaacgtg
    19141 tgattgtaac cgtaaaataa ttgaacacaa cacccaataa aattaggctc atggaataac
    19201 actccatggg cctgtttatt tttgcacatc ggacctcaga caatctctat acacgtttaa
    19261 tgcttttttc agtcaaagga acataatatc ctttgccatt cggacccata gcaaatttat
    19321 tgcgaagatg aattagatta gggccgaatt tcgcctctaa ttgttcacgg aactcatata
    19381 ggatttccat gatataaata tcatgaataa tcacttctgt aatcggcatc accagacccg
    19441 gatgcaacgt ccatgcagcg cggctgtcat ctccaagtga aagtatgcca gctacgacct
    19501 ccctgtcgtc tacgctaagt acaatccacc gaccaccaat ttcagacgta atctcctcac
    19561 tcatatcatg gcggtaaaca gtagcgaaat caaaattaag ctccccatac gctacaatga
    19621 tcacttcaat tccttgttga gcgacttgct ctaaggcatc tcggaacacc tcggcatcct
    19681 ctttccaaat ttccaataaa attcggtgtt ttgctccctt taccccttca tttatgcgat
    19741 tgataatggc ttcatacccc gaaatattcc aaatattctc tctattttga aatgatgctg
    19801 tgtactgctc caaactctct tgcgccacat tgaaaatctg ctctgctttg cgttttctct
    19861 gggcaatcaa ttcatgtggc gggagcggca catacagagg cgtattttct tgactgacca
    19921 tgatatcccc acggctgacc attccagaca aaacctcgta aattttggat ctcggtaccc
    19981 cggaattcaa tgaaacggcg tatcccgaaa gtggagagct ttccaataag gtcacataag
    20041 ccttagcttc atattcacta aaattcaaat cccgtagaat ttctaatata tttggttttg
    20101 gtttcatgac gtcttctcct caattcgtgt tcatccttac tatatcactt ccccttgaca
    20161 aaaactgata tacacactat ctttttagta acgtttttat agtagtcact ttagtagcta
    20221 ctatcatcat ttcacag
//
