LOCUS       NZ_CP109848            20644 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Paenibacillus polymyxa strain K16 chromosome.
ACCESSION   NZ_CP109848
VERSION     NZ_CP109848.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 5978518)
  AUTHORS   Manfredini,A., Vitali,F., Sas,L., Trzcinski,P. and Canfora,L.
  TITLE     Genome sequence of the Paenibacillus polymyxa K16 strain isolated
            from soil of tomato roots
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5978518)
  AUTHORS   Manfredini,A., Vitali,F., Sas,L., Trzcinski,P. and Canfora,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2022) Research Centre for Agriculture and
            Environment, CREA - Council for Agricultural Research and Analysis
            of the Agricultural Economy, Via della Navicella 2/4, Rome 00184,
            Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: SPAdes v. 3.14.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 270.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 06:01:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,334
            CDSs (total)                      :: 5,275
            Genes (coding)                    :: 5,199
            CDSs (with protein)               :: 5,199
            Genes (RNA)                       :: 59
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 52 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 22 of 76
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:36:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4659424
            Orig. end                         :: 4680068
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109848.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Strain stored in SYMBIOBANK
            culture collection of the National Institute of Horticultural
            Research in Skierniewice (PL).
FEATURES             Location/Qualifiers
     region          1..20644
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="11"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20644
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20644
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10644](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10644)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    complement(513..522)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 17.75"
                     /tool="antismash"
     gene            complement(743..1732)
                     /locus_tag="OF859_RS20790"
     CDS             complement(743..1732)
                     /EC_number="2.3.1.180"
                     /GO_function="GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017427907.1"
                     /locus_tag="OF859_RS20790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="WP_023988162.1"
                     /sec_met_domain="fabH (E-value: 4.6e-137, bitscore: 454.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKLRPVGVIGTGKYVPEKILTNKDLEAIVETSDEWIVSRTGIQE
                     RHIAAPEQATSDLAYEAAIKALESAGMTAQDLDLIIVATVTPDMAFPSTACILQDKLGA
                     KGAAAFDLSAACSGFVYGLATATSFIKTGIYNNALIIGADCLSRITDYTDRNTCVLFGD
                     GAGAVVIGEVPEGRGFQSFDLGAEGAGGSLLKLEAGGSRLPASADTLENKQHYIYMNGR
                     EVFKFAVRVMGTATVDVLEKAGLSKDDIDLFVPHQANVRIIQSAMQRLDLPEEKVVVNV
                     HKYANTSAASIPLALVEAAEEGRMKEGDRVLMVGFGGGLTWGASVLVW"
     gene            complement(1741..2733)
                     /gene="plsX"
                     /locus_tag="OF859_RS20795"
     CDS             complement(1741..2733)
                     /EC_number="2.3.1.274"
                     /GO_function="GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="plsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017427906.1"
                     /locus_tag="OF859_RS20795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="WP_023988161.1"
                     /transl_table=11
                     /translation="MRIAIDAMGGDNAPEATVEGALSAAAEWKDTDITLVGDLERIESV
                     LNGRALPTNLSIRHASEMIDAQDEPVKAVRRKKDASMVVAGRMVREGEADAMISAGNTG
                     ALMTTGLLVVGRMEGIERPALAPMIPTIDDQGILALDLGANMDAKPEHLAQYALMGSLY
                     RQKVHGVSSPRVGLLNVGTEAGKGNELTKLAYPLIEQLPVNFVGNVESRDVLTGNCDVL
                     VCDGFAGNIMLKSLEGTAGAIFSLLKEQFTKSLKTKLAAALIMPELRSLKGKLDYKEHG
                     GAPLLGLSGLVLKSHGSSDGVAVKNAVRQARTALQNRLVESISKEISRK"
     gene            complement(2717..3313)
                     /gene="fapR"
                     /locus_tag="OF859_RS20800"
     CDS             complement(2717..3313)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="fapR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429980.1"
                     /locus_tag="OF859_RS20800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transcription factor FapR"
                     /protein_id="WP_007429980.1"
                     /transl_table=11
                     /translation="MPKRQRQQRLTQLIDDNPFVTDQELTRQLKVSIQTIRLDRMELGI
                     PELRERLKLMAERSYDQVRSLPLHEVIGDIVDLQLDRSGISIFEIKEEHIFSRTGIARG
                     HYVFAQANSLAVAVINDEIALTASADIRFVRPVHLGEKCIAKAYVRSNPEQKGKAKVEV
                     FAYVGEEMVFQGNFVIYRSTEEEYSEGGSQHADRH"
     misc_feature    3478..3496
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 23.59"
                     /tool="antismash"
     gene            complement(3596..3769)
                     /gene="rpmF"
                     /locus_tag="OF859_RS20805"
     CDS             complement(3596..3769)
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370634.1"
                     /locus_tag="OF859_RS20805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="50S ribosomal protein L32"
                     /protein_id="WP_007429979.1"
                     /transl_table=11
                     /translation="MAVPQRRTSKTRRDKRRTHFKLAVPGMVKCSECGELKLAHHVCKV
                     CGTYKAREIISQ"
     misc_feature    3954..3968
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.9"
                     /tool="antismash"
     gene            complement(3957..4475)
                     /locus_tag="OF859_RS20810"
     CDS             complement(3957..4475)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370633.1"
                     /locus_tag="OF859_RS20810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF177 domain-containing protein"
                     /protein_id="WP_023988160.1"
                     /transl_table=11
                     /translation="MQLFFRKVATSDGPFPIRESLNVSELVDDRADVTAVSPLETDLVA
                     VSLGQGLMSVEGTLTAGLDMLCSRCLSPMHEDLKIEFRERFKQTSSSVEEEDEDGDFIA
                     VTEDSFDIAPYCEELFVLHVPFAPLCSEDCQGIAPKTGQNWSIGSDDSNDGDTEKIDPR
                     LAGLKDFFK"
     misc_feature    4648..4661
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.76"
                     /tool="antismash"
     gene            4831..6066
                     /locus_tag="OF859_RS20815"
     CDS             4831..6066
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686975.1"
                     /locus_tag="OF859_RS20815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase"
                     /protein_id="WP_264453895.1"
                     /transl_table=11
                     /translation="MKTLGMIVEYNPLHNGHVHHWEESLRITGAQRTVAVMSGPFLQRG
                     EPAITDKWSRTEMALAMGVDLVLELPVAYAVQPAEWFARGAVALLHATGIVDALCFGSE
                     SGDIAPLRALARVLAAESAELKAGIAQRLSSGASYPAAYAGAAAALAAEGEDGAALAAL
                     MQQPNNTLGLHYLIALERLGSTIKPFTVARTGSGYHEAAAPTDGVIASATAIRRLLLNG
                     GPEAASPYIPAATLEILQREWQAERAPLHWEAFSQPLLNALITRSPQQLAELHEVTEGL
                     EHRLRQSLYTLPQPSVEALLAAIKTRRYTRTKLQRMCTHILLNHAKTDMTPAELAKGPG
                     YIRVLGFNRAGRELLARMKQTATLPVWVKLSAGSHPQLDWDTAAAAIYAAGMPRPAIRD
                     MYADYLRPPIMV"
     gene            complement(6056..7087)
                     /locus_tag="OF859_RS20820"
     CDS             complement(6056..7087)
                     /EC_number="3.4.21.-"
                     /GO_function="GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309848.1"
                     /locus_tag="OF859_RS20820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S16 family serine protease"
                     /protein_id="WP_023988158.1"
                     /transl_table=11
                     /translation="MKRINRKRAFFTTGYVLMLAAMVYVLVYMPTPYLIYGPGGANEIK
                     PMVRVQEGDSIERGTFMMTTVSARYANVIMLGLSKLDRNSEIQRKEDRLHGRSEDEYAA
                     EQVWYMGDSQSSAMEAAYTRAHVPYRIVPDYVYVFKVPGSNSVFEPGDEILELNGVRVT
                     DNRSIRKALEDEKPGTMAKVKLKRDGKLLTVQAPLTTIKDSETGKQRSGFGVSIATVQK
                     VQPKDGRKTIHFTSTDVGGPSAGLMFTMEIYNQLTPGDLSKGYRAAGTGTIDKDGQVGA
                     IGGAKYKIVAADRQDADLFFVPADNYKEAKAKAEQIGTRMKLVPVRKLGDALDYMEKLP
                     IKP"
     gene            7285..8568
                     /locus_tag="OF859_RS20825"
     CDS             7285..8568
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686973.1"
                     /locus_tag="OF859_RS20825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside recognition protein"
                     /protein_id="WP_023988157.1"
                     /transl_table=11
                     /translation="MQKEAHSAPYWTTVLLGLGAILLVLAVVASPEKVFQASTQGLKLW
                     WSIIFPAMLPFLMLSEMLIAFGLVHGLGVLLEPFMRFWFRLPGRSGWVLALGLTAGFPA
                     AAEAVRQWAQQEDMTAFQLRRLTAIAHFCNPITILLVIGTGLLHNAAAGVMLLAVHWIS
                     GLLAGWITARLPVPSKELQKRETPAALRRTSVSRTSPSLIRSAWEATRSARERDGRSFG
                     KLLGETVSHAVQTLMVTGGFIIFFSVLIRLLSIYTGQGSLSFIWPAWMEIHLGSYEISR
                     LPFDLRTQAALISAVLGWGGLCGWLQITAVTRPSDKGITFTLSRVLHGIIAFGLTLATW
                     VPLSRIFSNTTPAYVSRSSVSSFAESVNESATHPVMAGASGLLTQIEGTSMYPWQLTVL
                     SIALLGTIIVTLLAFSFMTSWWSRHDSR"
     gene            complement(8531..9052)
                     /gene="coaD"
                     /locus_tag="OF859_RS20830"
     CDS             complement(8531..9052)
                     /EC_number="2.7.7.3"
                     /GO_function="GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="coaD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CTP_transf_like"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429971.1"
                     /locus_tag="OF859_RS20830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="WP_013309846.1"
                     /sec_met_domain="CTP_transf_like (E-value: 1e-22, bitscore:
                     79.1, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEHKPRIAVYPGTFDPVTMGHQDIIQRAARQFDLLIVAVLNNIS
                     KNPLFSLEERMELLRTVTRDIPNVEVDSFRDLTANYVRQKGAQVIVRGIRSVTDFEYEL
                     QLASTNHKLNPDAETIFMMTNPAYSYLSSSMVKEIAHFDGKVVDLVAPEVEDALRAKVN
                     ANRGGSTMKS"
     gene            complement(9045..9686)
                     /gene="rsmD"
                     /locus_tag="OF859_RS20835"
     CDS             complement(9045..9686)
                     /EC_number="2.1.1.171"
                     /GO_function="GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309845.1"
                     /locus_tag="OF859_RS20835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="WP_023988156.1"
                     /transl_table=11
                     /translation="MRVVSGSAKGRPLKAVPGTGTRPTTDKVKEALFSMIGPYFDGGVA
                     LDLFAGSGGLGIEALSRGMDKAVFIDMESKSIDVIKENLRKTGLEGQAEVFRNDAGRAL
                     KVLAKRGALFDAVFLDPPYRLKHGDELMSRMAELNLLRSDAIIVLEYESGHEYPESFGP
                     FEQVRKAVYGETALSIYHFAADVLSDVVEHTEAEGVKFGISESDGEDHHD"
     misc_feature    complement(9935..9948)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.4"
                     /tool="antismash"
     gene            complement(10001..10123)
                     /locus_tag="OF859_RS20840"
     CDS             complement(10001..10123)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370626.1"
                     /locus_tag="OF859_RS20840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_023988155.1"
                     /sec_met_domain="TIGR04223 (E-value: 1.4e-08, bitscore:
                     32.9, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMRKMVYGLATSLSMFATFAVSVASYVYVYQGDTPEELLK"
     gene            complement(10129..10644)
                     /locus_tag="OF859_RS20845"
     CDS             complement(10129..10644)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016819262.1"
                     /locus_tag="OF859_RS20845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_023988154.1"
                     /sec_met_domain="AgrB (E-value: 1.6e-17, bitscore: 61.7,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEAMAFKLANQIKRTVPDHPASVAVLKYALALILNASMIIVLTL
                     FASIFTGRMVEAVMILVAFALLRQISGGIHLKTGMACVLVTSAAFTYISCMALNSWWTN
                     LFTGIALILVLCFAPSRIEKQSRIPVQFYPLLRLLSSLLVCTNFVIGSSVVAIAFLVQS
                     LTLIRGRR"
     gene            complement(10637..11269)
                     /locus_tag="OF859_RS20850"
     CDS             complement(10637..11269)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370624.1"
                     /locus_tag="OF859_RS20850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_023988153.1"
                     /transl_table=11
                     /translation="MLLKPIGFLLFSTLEGVAVFALILSIFKVKMTPYLWQAIFVNLIM
                     NLQSYMLREEFSFSYLVPVVNMLLFIFLLATVVKIPIVWSGIMTVTGYFAYAVVQSVFL
                     KVMFGNLPVSELQDGSLKGYLLQVVSAAVGLLISLMLYRKGIGFSPNFKKLKFKEEYGI
                     VITLIILSLISTSIVLYYNEVWLNMIFFALVSGFFIYYAIGKEYRDD"
     gene            complement(11441..12559)
                     /locus_tag="OF859_RS20855"
     CDS             complement(11441..12559)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1088: acyltransferase 3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425759.1"
                     /locus_tag="OF859_RS20855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyltransferase"
                     /protein_id="WP_023988152.1"
                     /transl_table=11
                     /translation="MPEAKGKMEDDKLQFVDTLRALAIIGVLLVHVSQHVDGLNGWLQK
                     GLSIGAKGVALFYMASAFTLFLSLSRRSGDKRERTSAYLLRRFFRIAPLYYVMLGIYLV
                     VNGTGPRFWLGDQEGVTAANIAAHFLFLNGLNPYWINSIIGVEWSIAVECMFYLFIPLL
                     FKLIRSIRHATWFVVMALVVSFGLNAVFAQYPWINDHSLWGHYLYLWFPNQLPVFGLGI
                     LLFFIWKDERHWKSIDRVSGGWLLVSVLFAFFGGMTDYLIGIGLLLGAYALYHWQPIWL
                     LNRVWSWIGRLSYSMYLTHMLVLGLIVQVKLPFAPLVSLTLMFIMTLLFTIGLSWFTYS
                     WIEQPGIRWGKKLISRMKSVHKQGDSVKESVA"
     misc_feature    complement(12763..12775)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 20.68"
                     /tool="antismash"
     gene            complement(12872..14689)
                     /locus_tag="OF859_RS20860"
     CDS             complement(12872..14689)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1038: phenylalanine-specific permease"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281028.1"
                     /locus_tag="OF859_RS20860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="APC family permease"
                     /protein_id="WP_023988151.1"
                     /transl_table=11
                     /translation="MLSKVKRLLIGRPRKSTALEDEKLNKLKALAILSSDALSSVAYGT
                     EQILLVLITAGFAALWYSIPISIAVLGLLIILILSYRQTIFSYPGGGGAYIVAQDNLGK
                     SPSLIAGGSLLVDYILTVAVSSSAGTDAITSAFPSLHDHRIAIALIMIIFLTIMNLRGV
                     TESASVLAVPIYLFVVAIFVLIISGIIHYVAGGAHAAAPQFGATVSNVSLFLLLKAFSS
                     GCSALTGVEAVSNAIPNFRKPAAKNAATTLMMMGLILGCMFIGISLLAYWYGVRPNPHE
                     TVISQIANATFGRGVMYYIIQGVTALILFLAANTAYSAFPLLAFMLAKDKYMPHMFMVR
                     GDRLGYSNGILFLSIFSALLVIVFGGNTENLIPLYAVGVFIPFTLSQLGMMIRWIKLKP
                     PGWIVKLAINTVGMLTTLSITLIFIFTKFSQVWVIFIFLPLVLYFFMKINRHYKNTAEQ
                     LRIDITKDKPMVKGNTIIIPVAGITRVVMNTISYAKTLSDNVVAVYVGVDDEAIQKMEQ
                     KWEEWDVGIRLVVLRSRYRSIINPLRKFIDTVEWKKADEDHITVLIPQFITKHWWENIL
                     HNQTSLLMRAYLINYKDVIVTTVPFHLNK"
     gene            complement(14883..15719)
                     /locus_tag="OF859_RS20865"
     CDS             complement(14883..15719)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686969.1"
                     /locus_tag="OF859_RS20865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_076294885.1"
                     /transl_table=11
                     /translation="MILTPHHLRFFLTTREHSLPLFIESIGFNSRQEDVSRPEGYPCFH
                     WIQTVSGEGMFTFKGGTFRLGEQSGVLLLPGESHAYRRATKVWRTLYITFDGPIAAAVL
                     TALGLKHTRGYHWDPDSELHSFGETMLHSMVSERDLSGLDASANMYRFLTLLRKYGQHS
                     SMPSLSHNVERLTPVLAFMEQNYASPDVGLGDMAVMMNVSSRHLNTLFKQAFGVTSYAY
                     LIVIRLRKAKEMMTEYPQLTVKEISERVGFRDASHFVATFRRAEGITPERFKLLYT"
     misc_feature    15826..15839
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 21.57"
                     /tool="antismash"
     gene            15858..17885
                     /locus_tag="OF859_RS20870"
     CDS             15858..17885
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686968.1"
                     /locus_tag="OF859_RS20870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-galactosidase"
                     /protein_id="WP_264453896.1"
                     /transl_table=11
                     /translation="MISSKLPKVFYGGDYNPEQWDEATHQEDLRMFRQAGIDIATVNVF
                     SWAKIQSDEITYHFEWLDQLIDSLYESDIFVCLATSTAAHPAWMATRYPDVLRVDADGR
                     KRKFGGRHNSCPNSPTYRKYAEQMADRIAERYKNHPAILVWHVSNEYGGDCYCDNCAAA
                     FRVWLKQKYGSLEELNRVWNTSFWGHTFYDWDEIVPPNNLSEHWGENHSCFQGISLDYA
                     RFNSDSMLDCYLLEYNAIKKHVPDAVVTTNLMGFFKQLDYFKWAKYMDIVSWDSYPGAN
                     TPISYTAMAHDLMRGLKDGQPFMLMEQTPSQQNWQAYNSLKRPGVMRLWSYQAVAHGAD
                     TVMFFQLRRSIGACEKFHGAVIEHAGHENTRVFREVAQLGSELGALGNRLLDATVNAKV
                     AILFDWDNWWALEKSSGPSVALKYMDQVHKYYTPLFRRSVQVDLVSVDADFQKYDLIIA
                     PVLYMVKPGTAEKLEQFTEHGGTFVTTFFSGIVNENDLVTTGGYPGKLRNLLGIWVEEI
                     DALLPESHNRIVLKEKVGVLQGEYKCGLLCDLLHSEGAEVLAEYGDDFYQGMPVLTRNR
                     FGQGEAWYVASDPEEAFLDGLLGYICEQKGIPSLLRAPAGVEATVRTKDGKSYLFVLNH
                     NAQTQQYHLDDQKGNELISGKYLEGEISIEGYGFHVLELS"
     gene            17955..19181
                     /locus_tag="OF859_RS20875"
     CDS             17955..19181
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016819257.1"
                     /locus_tag="OF859_RS20875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_023988148.1"
                     /transl_table=11
                     /translation="MKRLLWIGSLSYFLIGLAHVVVGSLLPVLLEHYGRNYTDGGSLIF
                     AQFSGFLAGVLLSPWLARSFGKRRSLVFALLLLCAAEVLYSLLPPWGWLYAVGATAGFG
                     FGMVETLIGTIIISGITQGTGAAMSRLEVFFGIGALAMPAIASQLIALGWWRLAFPIIS
                     MFAAITIIAWLRGSFGTLDAVLDQQEHQGDNHTRSTNSPKSPTVEASKGSPIGNWKLLA
                     LFIVFFFIYVGTEMSLANFLPSMFIERLGLTQAEAALSVTCFWLAMATGRLFTGYIADR
                     YGYGVFVALSSLAATVLLCVFPLIKGTEGAFILIVLLGLGMSGIFSIALVFASKMMPGT
                     EESTTSLLIGAGGVGGALLPLWLGNSMDRGGAVSSAWLLAGFACILCLLGGILYALYVR
                     KKRLQTASS"
     gene            19392..20027
                     /locus_tag="OF859_RS20880"
     CDS             19392..20027
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309838.1"
                     /locus_tag="OF859_RS20880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_023988147.1"
                     /transl_table=11
                     /translation="MGHPELKRKKGRKKNVFVRAWFDKLEKKFEEKPPKRKVRSKRKGV
                     GKDIIVKKQLVSVNINHSALGPEGDPGTIGDLAQFGYIYNVKPQVVPIEGDVILDTNGI
                     LTPGIAHVPGTTQIAVSDAGKYEVHFSVSGVEPNQFAIFINGALAVGTVYGSGAGTQQN
                     TGQAILALACGDVLTLRNHSSTTAVTLQTQAGGTQASINASVLIKKLR"
     misc_feature    20452..20465
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.49"
                     /tool="antismash"
ORIGIN
        1 ttacagcctc caacttgccg gagagcttgg tcgctgcttc cttcatcaat gacgagtgga
       61 aaggaccgct aacctccaga gtaatagcgc gcttgcctcc tgcttccttc acacgttcag
      121 cgacagcagc tacgccctcc ttcgtaccag atacaacaat ttgtccagga caattgatgt
      181 tggctagttc gaccagttga ccagacttcg taatatcacg gcacaatgct gccaatgctt
      241 cacgatcagc tcccaacaca gcagccatcc cgccttgccc atcaggaatg gcctgctcca
      301 tgaactcgcc acgtgtacga acgatttcaa ccgcatcctc aaatgcgagg acaccggaag
      361 cgactagcgc gctgtattct cctagactgt gtccagccac ataatcggga tgaatgccca
      421 ttttttcctt gaacgcttca tacaacgcaa tgctcgccgt taacagtgca ggctgcgtat
      481 tggatgtttg cttcaaagct gtgtccggcc cttcaaaaat cagtgtgctt agtgcgaacc
      541 ctaaccgttc gtccgcttgg cgaaatactt ctgttgcagc tggaactgct tcataagcat
      601 ctttagccat acctacagct tgtgaaccct gaccgggaaa tataaatgcc gttttgccca
      661 tcgtgtatgc acctctctgt atccaatgct tgtcctgtaa aacactacac aaattcaata
      721 ttatatcaaa aattccattt tatcaccaga caagaaccga cgctccccag gtcaagccac
      781 cgccaaagcc caccattaat acacggtcgc cttccttcat gcggccttcc tcagctgctt
      841 ccacaagagc gagagggatt gatgctgccg atgtattcgc atatttatga acattaacaa
      901 ctaccttttc ctctggtaaa tccagacgtt gcatcgccga ttggatgatg cgaacattgg
      961 cttgatgcgg aacgaacaga tcaatatcat ctttggacag ccctgctttt tccaacacat
     1021 cgaccgttgc cgttcccata acacgaaccg cgaacttgaa cacttcgcgt ccgttcatgt
     1081 aaatatagtg ctgtttattt tccagtgtgt ctgcactcgc aggcaaacgc gagcctcctg
     1141 cttccaactt gagtaagctg cctccggcac cctcagcccc gagatcaaag gattggaatc
     1201 ctctaccttc aggcacttcg ccaatcacta cagctcctgc accatctccg aatagaacac
     1261 aggtattgcg gtcggtataa tctgtaatcc gagaaagaca gtctgcccca atgattaatg
     1321 cattattata gatacctgtc ttaatgaagc tcgttgccgt agccagtccg tatacaaaac
     1381 ccgagcatgc cgcagacaaa tcgaaagctg ctgcgccttt ggctcccaat ttatcctgaa
     1441 gaatgcaggc agttgacgga aacgccatat ccggtgtaac cgtagctaca ataattaaat
     1501 ccagatcctg tgccgtcata cccgcggact ccaacgcctt aattgctgct tcataagcta
     1561 aatcagatgt agcctgctct ggagccgcaa tatggcgctc ctgaatccct gtacggctga
     1621 caatccattc atcacttgtt tctacgattg cttccaaatc tttattggtc agaattttct
     1681 caggtacgta tttccctgtt ccgataacac caaccggccg taatttattc atatcgtcac
     1741 tcacttcctg ctgatttcct tggatatact ttccaccaac cggttttgca aggccgtacg
     1801 agcctgcctt accgcatttt tgacggcaac gccgtcagac gaaccgtgac ttttcaggac
     1861 gagtccactc aaacccagca aaggagcccc gccatgctct ttataatcca atttaccttt
     1921 cagactgcga agctcaggca taatcaaggc tgccgcaagc ttggtcttca aggatttggt
     1981 gaattgctct tttaagagag aaaatatggc tccggctgta ccctctagcg attttagcat
     2041 gatatttcca gcaaatccat cgcacaccag tacatcgcaa ttcccggtta acacatctct
     2101 cgattcaaca ttacccacaa aattgacagg taactgctca ataagtggat atgccaactt
     2161 cgtcagttca ttgcccttcc cagcttcggt ccccacattt agcagaccca cccttggaga
     2221 agacacaccg tgaactttct gacgatacaa gctccccatc agtgcatact gggcaagatg
     2281 ctcaggcttg gcatccatat tggctcccaa gtccagcgct agaataccct gatcatctat
     2341 cgttggtatc atcggcgcga gagccggacg ttcaatgccc tccattcttc caaccaccag
     2401 caaccctgtg gtcattagcg cgcctgtatt acctgcggat atcatggcat ccgcttcgcc
     2461 ttctctgacc attcgaccag ctacgaccat cgaggcatcc tttttacggc gcactgcctt
     2521 tacaggctcg tcctgcgcat ctatcatttc ggatgcatgg cgaatgctga ggttcgtcgg
     2581 caacgcccta ccgttcagaa ccgattcaat tcgttccagg tcaccaacca gcgtaatgtc
     2641 cgtatccttc cattccgctg ccgcagatag cgcgccctct actgtcgcct caggagcatt
     2701 atctcccccc atggcatcaa tggcgatccg catgctgact tcctccttcg ctatactctt
     2761 cctccgtgga gcgataaatg acaaagttgc cttggaacac catttcttct cctacgtagg
     2821 caaagacttc cactttagcc ttcccttttt gttctggatt tgagcgtaca tatgccttgg
     2881 caatgcactt ctcacctaaa tgaaccgggc ggacaaagcg tatatccgct gatgcagtca
     2941 atgctatctc atcattaatc acggcaacgg ccaatgaatt ggcctgagca aatacataat
     3001 gcccccgcgc aatgccagta cgcgaaaaaa tatgttcctc tttaatctca aaaatcgaaa
     3061 taccgcttcg atccaactgt agatcaacga tatccccgat cacttcatgc aaaggcaagg
     3121 aacgaacctg gtcgtaggag cgctcagcca tcagctttag ccgttcccgc aattctggga
     3181 ttcccagttc catccggtcc agccgaatag tctgaatact taccttcagc tgtcttgtca
     3241 attcctgatc cgttacgaac ggattatcat caattaattg agtcagccgc tgctgtctct
     3301 gtctcttcgg caaacgttcg atgtcagtga cgcctccttt ccctacaata aactgtcgtt
     3361 cagacgacag ttcgggttac ttcacttcac tttctaacgg cgaaacttca agccgatttg
     3421 tcttcttgta tgtataaagg aacctcttcg gcccttcttt cttagaacct ggtactaaaa
     3481 cagtatatag catgctgacg taaaaaggaa gcatgcagac aaaaaaaagt agcgcctcac
     3541 tgaagtgaag cgctactctt cctgcgaaga ttccaaagaa tccgcttatc ggacattatt
     3601 gagagatgat ctctctagct ttgtacgttc cgcaaacttt gcacacatgg tgagcaagtt
     3661 tcaattcgcc gcattcggag catttcacca tacctggtac tgccagttta aagtgagtac
     3721 gacgtttgtc gcgacgagtt ttggatgttc tccgttgagg tactgccatt attcccacct
     3781 ccttaaccag atcacccccg catagcgggt atgacttctt atctcattca agactgaaag
     3841 aaaatgccag aagcaattat cttacagccg gtgaatcaag cctgcaacat ccacgcatcg
     3901 gttcagtaac tctccggcac aagcggatga tgatttttca gaattgaact caattctcat
     3961 ttaaaaaaat ccttaagccc tgccagacgc ggatcaatct tttccgtatc gccatcgtta
     4021 ctgtcatcgg agccaatact ccagttttgt ccggtctttg gtgcgattcc ctgacaatcc
     4081 tcactgcaca aaggcgcaaa cggcacatgc agtacaaaca gttcttcaca atacggtgca
     4141 atgtcgaagg agtcctccgt cactgcgata aaatcgccat cttcatcctc ctcttcaaca
     4201 ctcgaagagg tttgcttgaa ccgttcacgg aattcaatct ttaaatcctc gtgcatcggg
     4261 cttaaacagc gtgaacacag catgtccaga cctgcggtca acgtgccttc cacgctcatc
     4321 aagccttgac ccagactaac ggcgaccaag tcggtctcca gcggggaaac ggccgtaaca
     4381 tcagcacgat catccaccaa ttcactcaca ttaagagatt cacggatagg aaaaggaccg
     4441 tcacttgttg ctactttgcg aaaaaatagt tgcatcacta tcactccaaa caaacaaaaa
     4501 ttattatacc gactgatcga gtcgtttgtc aacacctttc actttacacc actcacaaat
     4561 ccaccggttt tccaatggtt cgacaagttt tatgcagaag caaaaatgca ttgtgctaaa
     4621 catgtaccga tgagtattcg tacccccgcc actcttcgtg tacaataatg cgagtataaa
     4681 gctttttcgt ccaggtctca atggtcttgt tcatgccgac gatgaaaaac agtatagctg
     4741 ccctgaaaac aatcattcac gggtacttaa ccattattat caaaatagtt acccaataag
     4801 ttactttaag aaaggagccg acatacggtc ttgaaaacac tcggtatgat tgtagagtac
     4861 aatcctctgc ataacggaca tgtccatcac tgggaggagt ctcttcgcat cactggtgca
     4921 cagcgcacag tggctgtgat gagtggccct ttccttcagc ggggtgaacc cgcgattaca
     4981 gacaaatggt cccgcaccga aatggcactc gccatgggtg tggacctggt tctggagctg
     5041 cctgtggcct atgccgtcca gcctgcagaa tggtttgccc gcggagcggt cgccctgctc
     5101 catgcgacgg gcatcgtcga tgcgctctgc ttcggtagcg agagcggaga catcgccccg
     5161 ctgcgcgcgc tggcccgcgt gctggcggca gagtcggccg agctcaaggc cggcatcgcg
     5221 cagcgcctaa gcagcggcgc gagctatccc gctgcctacg cgggagccgc agccgcgctg
     5281 gcggccgaag gagaggacgg cgctgcgctc gccgccctca tgcagcagcc caacaatacg
     5341 ttggggctgc attacttgat tgcgctggaa aggctgggca gcacaatcaa gcccttcacg
     5401 gtagcccgca cgggatcggg ctaccatgaa gccgcggccc ctacggacgg agtcatcgcc
     5461 agcgccacgg ccatccgccg tctgctgctg aacggcgggc ctgaggccgc gtcgccgtac
     5521 atacctgcgg cgacactgga gattttgcag cgcgagtggc aagccgaacg cgcgccgctg
     5581 cattgggaag cgttcagcca gccgctgctg aacgctctga tcacacgtag cccgcagcag
     5641 ctggcggagc tgcacgaggt caccgaaggg ctggagcatc gtctgcggca atcgctgtac
     5701 acgttgcccc agccttctgt ggaggccctg ctcgccgcga tcaagacaag gcgctatact
     5761 cgtacgaagc ttcagcggat gtgtacacat attttgctga atcatgccaa aaccgatatg
     5821 actccggctg agctggcgaa aggtccgggc tatatccgcg tgctcggctt taacagggct
     5881 ggacgcgagc tgctggcacg tatgaagcag acagccaccc ttccggtatg ggtcaagctg
     5941 tctgccggat cacatcctca gctggactgg gatacagccg cagccgccat atacgcagct
     6001 gggatgccac gtcccgccat ccgcgacatg tatgctgatt acttgcgacc gcccattatg
     6061 gtttgatggg cagcttttcc atataatcca gcgcatcgcc aagcttgcga accggaacaa
     6121 gcttcatccg ggttccaatt tgctcagctt tggccttcgc ctctttataa ttatctgcag
     6181 ggacgaaaaa caaatccgca tcctgacggt cagcagcaac tattttatac tttgcaccac
     6241 caatcgctcc aacctgaccg tccttatcaa tagtacccgt accggctgca cgatatccct
     6301 tactcagatc acctggtgtc agttggttat agatttccat ggtaaacatc aaccctgccg
     6361 aaggaccgcc aacatccgta gatgtaaaat gaatcgtttt acgtccatcc ttcggctgta
     6421 ccttctgtac cgtggcaata ctgacgccaa atccagaacg ttgcttgccc gtctcactgt
     6481 ccttgatggt cgttaaggga gcttgcacgg tcagcaactt tccatcacgc tttagcttca
     6541 ctttagccat tgttcccggt ttttcgtcct ccaaagcctt ccgaatggaa cgattatcgg
     6601 tcaccctcac tccgttcagc tccaatattt catctccagg ctcgaacacg ctgttcgagc
     6661 ccgggacctt aaatacatac acataatctg gcacaatccg ataggggaca tgggcacggg
     6721 tgtaggcagc ctccattgcg gaagattggg aatcccccat ataccatacc tgttctgccg
     6781 catattcgtc ttcacttctt ccatgcagac gatcctcttt gcgttgaatc tccgaattgc
     6841 ggtccaactt ggacaggccc aacatgataa cattggcata acgcgccgat accgtcgtca
     6901 tcataaaagt cccccgttcg atgctgtcac cctcctggac tctaaccatc ggtttgatct
     6961 cgtttgcccc acctggtcca tagatcagat aaggggttgg catatagacc agcacataga
     7021 ccattgcagc cagcattaag acataaccgg ttgtaaaaaa agctcttttc cggtttattc
     7081 gtttcattgc ttctccccct tacagcctaa cttctcctat cttacccaag tgcctctacg
     7141 ccacctgttc tcaagccttc cgaaaaagca tacattgttt acgacaacgt atacgaacaa
     7201 cctatcccaa gacaacgaac gtgaataaac gctggagaca gcatccgact ctccttagta
     7261 tatttatgag aggtgaattc tttgatgcaa aaggaagccc attccgctcc ttattggaca
     7321 accgtactgc ttggccttgg agccatttta cttgtactgg ctgttgtcgc ctcaccagaa
     7381 aaagtatttc aagcctctac tcaaggcctt aagctatggt ggagtattat tttcccggcc
     7441 atgttgcctt ttttgatgct gtccgaaatg ctcatcgcct ttggtctagt ccatggacta
     7501 ggtgtcctgc tggagccgtt tatgcgattc tggttccgtc tacccgggcg aagcggctgg
     7561 gtgttggccc tcggcctgac cgcaggattc ccggctgctg cggaagcagt gcgccaatgg
     7621 gcacaacagg aggatatgac cgcgtttcag cttcgacgtc taaccgctat cgctcatttc
     7681 tgcaatccga ttaccatcct gctcgtcatc gggacagggc tactgcacaa tgctgccgca
     7741 ggtgtgatgt tacttgccgt tcactggatt tccgggctgc tggcaggctg gatcacagcc
     7801 agactacctg taccgtccaa agaattgcaa aaacgggaaa cgccagctgc cctccggcgc
     7861 acctcagttt cccgtacttc tccctccctg atccgatccg catgggaagc tacccgctct
     7921 gccagagaac gggacggccg cagtttcgga aaactgttgg gcgaaaccgt atcccatgct
     7981 gtacagacgc tgatggtgac aggtggattc attattttct tttccgtact gattcgactg
     8041 ctgtccatat atacaggcca aggatcgctt tctttcatat ggcctgcctg gatggaaatc
     8101 catctgggtt cctatgaaat cagccgtctg ccctttgatc tccgtacaca agcagccctg
     8161 atcagtgctg tgttgggctg gggtggcctg tgcggctggc ttcaaattac cgccgtcacc
     8221 aggccttccg acaagggcat caccttcacc ctgtcacgag tgctccatgg gatcattgct
     8281 tttggactta ccttggcgac atgggtcccg cttagccgca tcttcagcaa tacaactccc
     8341 gcttacgtca gtcgcagttc tgtgtcttct tttgcggaaa gcgtaaatga atccgccacg
     8401 catcctgtca tggccggagc cagcggctta ttaacacaaa ttgaaggaac atctatgtac
     8461 ccctggcaac taaccgtact gtccattgca ctgttaggta caattatagt taccctgctg
     8521 gccttttcct ttatgacttc atggtggagc cgccacgatt cgcgttgacc ttcgcacgca
     8581 gtgcgtcttc cacctccggt gccaccagat ctacaacctt gccatcaaaa tgcgcaattt
     8641 ccttcaccat actggaactc agatatgaat aagctggatt ggtcatcata aagatcgttt
     8701 ccgcatccgg gttcagctta tgatttgtag aggctaattg tagctcatat tcaaaatcag
     8761 tcaccgaccg aattcctctg acaataacct gggccccttt ttgacgtaca taattagctg
     8821 tcagatcacg gaagctgtcc acttcaacgt tcgggatatc gcgagttacc gttcgcagta
     8881 actccattcg ttcctcaagt gaaaacagcg gatttttact aatattgtta agaaccgcga
     8941 caatcaatag atcaaactgt ctggctgctc tttgaataat atcctgatgc cccatcgtca
     9001 cgggatcaaa agttcccgga tatacagcaa tacgtggttt gtgctcaatc atgatggtcc
     9061 tccccgtctg attcgctgat gccgaatttg accccttcgg cttctgtatg ttctacgaca
     9121 tccgacaaaa catcggctgc aaaatgataa atagataacg ccgtttcacc ataaactgct
     9181 tttctgacct gttcgaacgg cccgaagctt tccggatatt catgtcccga ttcatactcc
     9241 agcacgatga ttgcatctga acgaagcaag ttcaactcag ccatacggct cattaattca
     9301 tctccgtgtt tgagacgata gggtgggtcc agaaaaacag catcaaaaag agctccacgt
     9361 tttgccagca ccttgagcgc tcgtccggca tcattgcgga acacttcagc ctgtccttcc
     9421 aatccagttt ttcgcaaatt ttctttaatg acatcaatgc ttttggattc catatcaata
     9481 aaaacagcct tgtccattcc ccggctaagc gcctctatac ccaggccacc actgccagca
     9541 aacagatcaa gtgcgactcc cccatcaaaa taaggaccta tcatgctaaa tagcgcttcc
     9601 ttgaccttat ccgtggtcgg ccgcgtccct gtgccgggaa ctgcctttaa cggccttcct
     9661 ttggcactac ccgataccac tctcacgctg aaactcacct attttctata tgtgttcata
     9721 accatataag attatgacgc tatcgtacca catgcaccgc cgtttgaaaa acttaggatg
     9781 aggcaggtat aaaaaaccat tccctgggca tccttaggat atcggcattc aaccaatgtc
     9841 gtagacaacc gcggagaaga cttacgtttc cccataagct cttcgtccgg tttctcctct
     9901 cccattcggg cgctctttaa tggagcgcct gatttttttg cataaaaaga agctgcttaa
     9961 tagcagctct atgtatcatt tttctatttc ggcattttgt ttatttcagc agttcctcgg
    10021 gtgtatcccc ttgataaaca tacacgtaac tcgccactga tacagcaaat gttgcaaaca
    10081 tagacaacga tgtagctaat ccatatacca ttttcctcat cattccactc acctccttcc
    10141 ccggatcaat gttaggctct ggaccaaaaa agcaatagcc actacagagg aaccgattac
    10201 aaaattagta catacaagca aggaggataa cagtcgcaag aggggataaa attgtactgg
    10261 aatgcgcgac tgcttttcaa ttctggacgg ggcaaaacat aaaacgagaa tgagagctat
    10321 tccggtaaac aggtttgtcc accaactatt taaggccatg caggatatgt aagtaaaagc
    10381 tgctgaggtc accaacacac atgccatgcc cgttttcaaa tgaatgcctc ctgatatttg
    10441 gcgcagcagg gcgaaagcga cgagaatcat tacagcctct accatccgcc ccgtgaagat
    10501 tgaggcgaat agtgttaaca cgatgatcat ggacgcattc agaatgagag caagtgcgta
    10561 tttaagtacc gcgacagagg ccggatgatc aggcacagta cgtttgatct ggttagcaag
    10621 tttgaaagcc atcgcttcaa tcatcccggt attcctttcc gattgcataa tagatgaaaa
    10681 atcccgaaac cagtgcaaaa aatatcatat ttaaccatac ctcgttatag tagagcacaa
    10741 tagaagttga aatgagcgac aggataatca gcgtgatgac aatgccatat tcctctttga
    10801 actttagctt cttgaaatta ggggaaaagc cgatcccttt tcgatacaac atcaatgaaa
    10861 taagcagtcc tactgccgcg cttaccactt gtagcagata gcccttcaag gacccatcct
    10921 gcagctcaga aaccggaagg ttgccgaaca tgacttttag gaataccgac tgaacaacgg
    10981 catatgcaaa ataacctgta accgtcataa taccggacca cacaatcgga atcttcacga
    11041 ccgtagcaag taaaaaaata aaaagcagca tatttacgac aggcaccaaa tacgaaaagg
    11101 aaaactcctc tcggagcata taactttgca aattcataat caggttaaca aaaatggcct
    11161 gccatagata aggggtcatt ttaactttaa aaatactcaa aatcagcgca aaaacagcta
    11221 ctccctcaag cgtggagaaa agcaaaaagc cgattggctt caaaagcatg cgtgcatctc
    11281 ctttacaatt ccaacaaacc tcatcggtgt attcaaaaac ttggatgtac atcgaatata
    11341 aagggctggg agctactttt caagaggtct tttcaattat ttcatagatt tattcaagca
    11401 cttcaaccaa atgttatcgg atgctgtttc aagttgcagc ttaggcaaca ctttctttga
    11461 ccgaatctcc ctgcttgtgc acagacttca tacgtgagat aagctttttc ccccaacgga
    11521 tgccgggctg ttcaatccac gagtaggtaa accaggatag tccgatggtg aatagcaacg
    11581 tcatgataaa cataagtgtg aggctgacta ggggagcaaa aggtagttta acctgtacga
    11641 ttagtcccag aaccagcata tgcgtcaaat acatgctgta actaagacgg ccgatccatg
    11701 accacacccg attaagcagc cagatcggct gccagtgata caaagcatac gctcccagca
    11761 gcaacccgat acctatgaga taatcggtca tacctccaaa aaatgcaaat agcacagaaa
    11821 ccagcagcca acctccactg acccggtcta tacttttcca atgccgctcg tctttccaaa
    11881 tgaaaaacaa caatatacct agtccaaaga caggcaattg attcggaaac cataaataca
    11941 gatagtgccc ccataaagag tgatcattaa tccatggata ttgagcaaat accgcattta
    12001 gcccaaagct taccacaagt gccatcacaa caaaccaggt cgcatggcgg attgatcgga
    12061 tcagcttaaa taataggggg ataaacagat aaaacataca ttccaccgca atggaccatt
    12121 ccacaccaat aatgctgttg atccagtacg gattcagacc attcaaaaac aggaaatgag
    12181 ctgcgatatt ggcggcagtg actccctcct gatctccaag ccaaaatcgt ggacctgtcc
    12241 cgttcacaac caaatagatt cctaacatca cgtaatacaa aggagcaatt cgaaaaaaac
    12301 gacgcaacag gtaagctgaa gttctttccc tcttgtcacc tgaacgtctg ctcaaggaca
    12361 gaaatagtgt aaaagcgctg gccatataaa ataaagcgac tcctttagct cctatactca
    12421 agcctttttg caaccatccg tttaatccgt ctacatgctg gctgacatgc acaagtaaaa
    12481 ctccaataat agccagcgcc ctcagcgtat ctacaaattg cagcttgtca tcttccattt
    12541 tccccttggc ttcgggcatc gtatccctcc ataacagttt cgaatgatac atttcgtatc
    12601 tataatacac aaaatgtatc accaaagtaa atatctccgt tatgcaaaaa aacaccctcc
    12661 gaccgcaaag aaatgcggcc aaaagatgtt cattttgtcc ttgctatagt cagctcacgc
    12721 aaatgagtat gggctaattg gcagtgactt tatttcagga ttttgcaaaa ttcgattcca
    12781 gtcgctccga agtgcaagat atagacgaac taaagcattt cgatctatat cctgttcatc
    12841 ggaagtcgct tatgggattt ttgcaaaatc ctcatttgtt taaatgaaaa gggacggtcg
    12901 ttacgatcac gtctttgtaa ttaatcaaat aagctctcat cagtaagctg gtctgattgt
    12961 gtagaatatt ttcccaccag tgcttggtga tgaattgtgg aatgagaaca gtgatatggt
    13021 cctcgtctgc ttttttccac tctactgtat cgatgaactt gcggagcgga ttaatgatgc
    13081 tgcgataacg ggatctcagc actactaaac gtatgcccac gtcccactct tcccacttct
    13141 gttccatttt ttgaatcgcc tcatcatcca cacccacata tacggcaacc acattatccg
    13201 acaaggtttt ggcatagctg atcgtattca taacgactcg cgtaatacct gccaccggaa
    13261 taataatcgt attgcctttc accataggtt tatccttggt aatatcaatg cgcagttgtt
    13321 cagccgtatt tttgtaatgc ctgttgatct tcataaagaa atacagaacc aaaggcagaa
    13381 agataaatat tacccacacc tggctaaatt tggtgaaaat aaagatgagc gtaatcgaca
    13441 gcgtggtcag catcccaacc gtattaatag caagtttgac gatccagcct ggcggtttga
    13501 gcttgatcca gcgaatcatc atgccaagtt gcgagagcgt gaacggaata aataccccta
    13561 cggcataaag cgggatcaga ttttctgtgt ttccgccaaa tacgataacc agcaatgccg
    13621 agaagatact cagaaacagg ataccattag agtatccaag ccgatctcca cgaaccatga
    13681 acatgtgcgg catgtatttg tccttcgcaa gcataaaagc aagcaatgga aaggccgaat
    13741 aggctgtatt cgccgccaaa aacaagatga gcgccgttac gccctgaatg atgtaataca
    13801 tcacaccgcg cccaaaagtg gcgttcgcaa tttgcgaaat gaccgtttca tgcggattag
    13861 gacgaacacc gtaccaataa gccagcaaac taattccgat aaacatacac ccgagaatca
    13921 aacccatcat cattagtgta gttgctgcat ttttggcagc tggcttgcgg aagttcggaa
    13981 tggcattcga gacagcctct accccagtta acgccgagca acccgagctg aacgccttca
    14041 gtagcaggaa caggctgacg ttggataccg tagctccaaa ttgcggtgct gcagcatgag
    14101 ctccccctgc cacgtaatga atgataccgc taataatcaa aacaaaaata gcaacaacaa
    14161 ataagtaaat cggtacagcc agcaccgagg cagattctgt caccccgcgc aagttcataa
    14221 tagtcaaaaa gataatcata atcagagcaa tggcgatccg atgatcatgt aacgacggga
    14281 acgcagatgt aatggcatct gtacctgctg atgagctaac tgctaccgtc agaatataat
    14341 cgaccagcaa agaaccacct gcaatcagac tcggactctt tcccaaattg tcctgtgcaa
    14401 caatgtacgc tccgccacca ccaggatagg aaaatatggt ctgtctatag gacaggatca
    14461 gaatgatcag caaacccaat accgcaatgg atataggaat ggaataccat agggcagcga
    14521 agccagctgt aattaaaacc agcagaattt gctccgttcc gtaagctaca gaggacaagg
    14581 catctgagga cagaatggcc agtgccttga gcttattcag tttctcatct tcaagggccg
    14641 tcgactttcg cggacgaccg attaataatc ttttgacttt actcagcatt tcgttgactc
    14701 cttctttgga tgagggtttt gcaaaagaac tatggatctt atgtttatta tgtcacctgc
    14761 acatcagttt gatgaaacaa atgaactatt attttatact ccctcatcct gaaaaaaaca
    14821 ataaggaatg ctccctaaaa agagcattcc cgagtaagaa agagtatttc gataaaatac
    14881 ccttatgtat acagtaattt gaacctttcc ggggtaattc cttcagctcg gcggaaagtt
    14941 gccacaaaat gactggcatc tcggaatcct actcgctccg aaatctcctt tactgttaat
    15001 tgcggatatt ccgtcatcat ttccttggcc ttccgcagcc gaattacaat cagataagca
    15061 taagaagtca caccaaacgc ttgcttaaat aatgtattta aatgacggga gctgacattc
    15121 atcataactg ccatatcacc taggcccaca tctggactgg cataattttg ctccataaag
    15181 gccagtacag gggttaaacg ttccacatta tgggataggg aaggcatcga gctatgttgt
    15241 ccatatttac gcagtagagt cagaaaacga tacatattgg ccgaagcatc taatccagac
    15301 aaatcacgtt cactaaccat ggaatggagc atagtttccc caaagctgtg cagctcgcta
    15361 tccgggtccc aatgataccc acgcgtatgc ttcaatccca gtgcagtcaa tacagcagcg
    15421 gcgatggggc cgtcaaatgt aatgtataac gttcgccaaa ccttggtcgc ccgtctgtat
    15481 gcatgggatt ccccgggcag cagcagaacc ccggactgct ctcccagacg gaaggtcccc
    15541 cctttaaatg taaacatccc ttcaccagac accgtctgaa tccagtgaaa gcacggataa
    15601 ccctctggac gggacacatc ctcctgcctg ctgttaaatc cgatactttc aataaaaagg
    15661 ggtaaagagt gctcacgcgt agtcaggaaa aatcttaaat gatggggagt taaaatcata
    15721 aaaagcacct ccggcgtatg cgggtatata ttcttcatgt agatccatat tgttatatac
    15781 acgttcattt tattatattt aaaagcagtt atttgtatta taagatagaa ttattcttat
    15841 atacagaggt gattggcatg ataagcagca aacttcctaa agtattttac ggtggagatt
    15901 ataatcctga gcaatgggat gaggccacgc atcaggaaga tttacggatg ttcaggcaag
    15961 cagggattga tatcgccacg gtcaacgtat tctcatgggc caaaattcag tccgacgaaa
    16021 tcacatatca ttttgaatgg ctggatcaat tgattgacag tctttatgaa agcgatatct
    16081 tcgtttgtct agctactagc acggcagcgc accctgcttg gatggctacc cgctaccccg
    16141 acgttctccg ggtagacgct gacggacgga agcggaaatt cggtggccgc cacaactcct
    16201 gtccgaacag tccaacgtat cgaaaatacg cggagcagat ggctgatcga attgcagaac
    16261 gatacaaaaa ccatcccgcg atcctcgtgt ggcacgtgtc caatgagtac ggcggggatt
    16321 gctattgtga taactgcgcg gccgcattcc gggtgtggct caagcaaaaa tacggatcac
    16381 tggaagagct aaaccgggtg tggaatactt ccttctgggg tcacaccttc tatgattggg
    16441 atgaaattgt accgccaaat aatctgagcg agcattgggg agaaaatcac tcctgctttc
    16501 agggcatatc cctcgattat gcgcgcttca attcagacag tatgctggat tgctacctat
    16561 tggaatataa cgcgattaaa aaacatgttc ccgatgccgt ggtaacgacc aatctgatgg
    16621 gtttttttaa gcaactggac tattttaaat gggctaagta catggatatt gtatcttggg
    16681 acagctatcc cggggccaat actccgataa gctatacggc gatggcgcac gatctgatgc
    16741 gcggattgaa ggatgggcaa ccctttatgc tgatggagca aacaccgagc cagcagaact
    16801 ggcaggcgta taattcgctt aaacggcccg gcgttatgcg gttatggagc tatcaggcag
    16861 tcgcgcatgg cgcggacacg gtcatgttct tccagcttcg ccgctcgatc ggcgcttgtg
    16921 agaaattcca cggggccgtc attgagcatg ccgggcatga gaacacacgt gtatttcgcg
    16981 aggtggcaca gcttggcagt gagcttggcg cattaggcaa caggctactt gacgcgaccg
    17041 ttaatgccaa agtagccatt ttattcgatt gggataattg gtgggctttg gaaaaatcaa
    17101 gtggcccttc tgttgccttg aaatatatgg atcaggtaca caagtattat actcccctct
    17161 tccggcggag tgtacaggta gacctggttt ctgttgatgc agatttccag aaatatgatc
    17221 tcattatcgc gcctgtacta tacatggtca agccgggtac cgccgagaag ctggaacagt
    17281 tcacagagca tggcggcacc ttcgtaacga cctttttcag tggcattgta aatgaaaacg
    17341 atctggtcac aaccggagga tatcctggta aactacgtaa cttgctcggg atctgggtgg
    17401 aagaaattga cgcccttctg ccggaatcac acaatcgcat tgtattgaaa gaaaaggtgg
    17461 gcgtattaca aggtgaatat aaatgcggtc tgctctgcga cttgttgcat agcgaaggcg
    17521 ccgaggtgct ggcagaatac ggcgatgatt tctatcaggg catgccagtg ctgacacgca
    17581 atcgtttcgg tcaaggtgag gcatggtacg ttgcttcaga ccctgaagaa gctttccttg
    17641 atggcctgct gggctacatt tgtgagcaaa aagggattcc atccctgctg cgcgctcctg
    17701 cgggtgtaga agccactgta cggaccaaag atggaaaatc atatctattc gttctgaatc
    17761 ataacgccca gacgcagcaa tatcatctgg atgatcagaa agggaatgag ctgatcagcg
    17821 gtaaatacct ggaaggagaa atttctattg aaggctacgg ctttcatgta ctggagctat
    17881 cataatcact cagatttgac tcatgcagtt tagcttgccg catgagtcaa atctaggtat
    17941 caaaggagtt catcatgaaa cgactgctat ggatcggcag cttatcttat tttttaatcg
    18001 gcctggcaca cgtggttgta ggctccctac tgcctgtgtt gttagagcat tacggtagaa
    18061 actatacgga tggcggaagt ctaatcttcg ctcaattttc cggttttctg gctggagtcc
    18121 tgctgtcgcc gtggcttgcc cggagtttcg gcaaacgccg aagtctggta ttcgccctgc
    18181 tcctgctatg cgcggcggaa gtgctgtact cattgctgcc gccttggggt tggctatatg
    18241 ccgtcggggc aacagccggg tttggcttcg gtatggtaga aacactcatt ggcactatta
    18301 ttatcagcgg tattactcaa ggaacgggcg cggctatgag ccgtctggaa gtcttttttg
    18361 ggattggcgc tttggctatg cccgctatcg ccagccagct cattgcactc ggttggtggc
    18421 gattggcctt tcccatcatt tccatgtttg cagccatcac cattatagct tggctgcgcg
    18481 ggtccttcgg cacgctggac gctgtattag accaacagga acaccaaggt gacaaccata
    18541 cgcgttccac taactcaccc aaatccccca cggtggaggc ttctaagggt agccctatag
    18601 gcaattggaa gctacttgca ctatttatcg tctttttctt tatctatgta gggacagaga
    18661 tgagcctggc taatttcctg ccctccatgt tcatcgaacg gctgggtctc acacaagccg
    18721 aagcagcact tagtgtcacc tgcttctggt tagcaatggc taccggccgg ttattcacag
    18781 gctacattgc cgatcgatac ggctacggtg tctttgtcgc cctgagctca ctggcagcga
    18841 ccgtgctgct gtgcgtgttc ccgctgatca aagggactga aggcgccttt atccttattg
    18901 tactgctggg gctgggcatg tccggcatct tctccatcgc acttgttttt gccagcaaaa
    18961 tgatgccggg tacagaagaa tctaccacta gcctgcttat cggcgcaggt ggagttgggg
    19021 gagctctgct gcctttatgg ctcggaaata gcatggaccg tggtggagca gtctcatcag
    19081 cgtggttgct ggcaggcttc gcctgtatcc tgtgcctgct tgggggcatc ttgtacgccc
    19141 tatatgtgcg caaaaaacgg cttcaaaccg cttcatcatg agactcgtct ctactggcgc
    19201 ctttgtctgt tccttggttg tcatccaagt gcacattcaa aggtgctttt ttacatacta
    19261 gacgaatgca ccttgtacag cagcccgttt ttagtcaaaa tagatcgttt cctatgaatc
    19321 acaggacaga tatccatgcc tcctctagat gaatggagta gtatacaaca aaccatatgg
    19381 aggtgtttgt attgggtcat cctgaattaa aacgaaaaaa aggccgtaaa aaaaatgttt
    19441 ttgtccgtgc ctggtttgat aagttagaaa aaaagtttga ggagaagccc ccaaagagaa
    19501 aagtccgctc gaaacggaaa ggggttggaa aagatatcat cgtcaaaaag caacttgtaa
    19561 gtgtgaatat taatcatagc gctttagggc ctgaagggga tcctggaacc ataggtgatt
    19621 tagcgcagtt cggttatatt tataatgtga agccccaagt tgttccgatt gaaggggatg
    19681 tcattttgga tacgaacggt atacttacac ctggaattgc tcatgttcca ggaactacac
    19741 agattgctgt atccgatgct ggaaaatatg aagttcactt ttcggtgtcc ggtgtggaac
    19801 ctaaccaatt cgctatcttt attaatggcg cactggctgt gggaactgta tacggctcag
    19861 gagccgggac acaacagaac acagggcaag ctatcctcgc tctggcatgc ggtgacgtcc
    19921 ttactctgcg aaaccatagc tctactactg ctgttaccct gcaaacacaa gccggaggaa
    19981 ctcaagccag tataaacgct tctgtcctca ttaaaaaatt gagataggct tgaactcccg
    20041 caaagtctca gtgtactaaa aagggtttat ccatacagcg aggataaacc ctgaatattt
    20101 ttaaaaggcc ataatgatcc ctttgtcatt ggcatacatt gaactaatcc ctctggtgga
    20161 cacaatatga atatctttaa attgatagcg tttcttcagc gcattcatta atttttccgc
    20221 cagcccagga ttattgcagt gagtaatgac gatgctttct ccttcggtag cccggccact
    20281 tttttcaata gtatctgcca gcttttcgat aatttgattc tgaccgcgtg cttgagtaaa
    20341 aaatgcgata ttcccgtcac catccgagcc taaaataggc ctgatatgca agagtgaaag
    20401 tatctttcca gtgattttat tcattcgtcc gttcttaacc aaattatcca gattttctaa
    20461 aacaaagtac gttttcatct tgctgataaa gctctccaag gaagaaataa tttcgctttt
    20521 atgatagccc tcgtcgatta gcttgcgtag ttttagagct aacaagagct gtccagccga
    20581 agcgcttttc gaatcaaata catgaacatc cgccccctct tcttccgcca tatcttttcc
    20641 tacc
//
