LOCUS       NZ_CP107567            11437 bp    DNA     linear   CON 18-NOV-2023
DEFINITION  Streptomyces peucetius strain NA0869 chromosome, complete genome.
ACCESSION   NZ_CP107567
VERSION     NZ_CP107567.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces peucetius
  ORGANISM  Streptomyces peucetius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8050611)
  AUTHORS   Zhao,E. and Ge,H.
  TITLE     Cytochrome P450 Catalyzes Benzene Ring Formation in the Biosynthesis
            of Trialkyl-Substituted Aromatic Polyketides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8050611)
  AUTHORS   Zhao,E. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) School of Life Sciences, Nanjing University,
            No. 163 of Xianlin Avenue, Qixia District, Nanjing, Jiangsu 210033,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: unicycler(0.4.8) v. v. 3
            Assembly Name                     :: NJU_NA0869
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/18/2023 06:49:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,216
            CDSs (total)                      :: 7,126
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 144 of 303
            Pseudo Genes (incomplete)         :: 227 of 303
            Pseudo Genes (internal stop)      :: 22 of 303
            Pseudo Genes (multiple problems)  :: 85 of 303
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:23:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6441254
            Orig. end                         :: 6452691
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107567.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from IFB-NA0869, Nanjing
            University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11437
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11437
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11437
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6437](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6437)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            754..2184
                     /gene="hemG"
                     /locus_tag="OGH68_RS29180"
                     /old_locus_tag="OGH68_29180"
     CDS             754..2184
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965516.1"
                     /locus_tag="OGH68_RS29180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29180"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_264248062.1"
                     /sec_met_domain="DAO (E-value: 1.3e-08, bitscore: 33.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVDTPAPHVVVVGGGIAGLAAAHRLLASGVRVTLLEATDRVGGK
                     LRSGEIEGVRVDLGAESMLARRTEGTGLARAVGLGDRLEPPATATSAVWTRDALRPMPK
                     GHVMGVPGDASALAGLLSPEGLARIEHEKDLPPTGIGDDVAVGAYVAERLGREVVDRLV
                     EPLLGGVYAGDAYRISMRAAVPQLFEAARSHGSLLDGVRDIQARAAAGQQTGGPVFMGL
                     DGGIGTLPGAVADAVRAQGGDIRTETPVLGLARAGAGWRVRTDRETLAADAVVLATPAW
                     SASALLAGESPAAAAELGHVEYASMALVTMAFRRADVTGMPDGSGFLVPPVDGRTIKAS
                     TFSSNKWGWVARGAGDLFVLRTSVGRYGEEQALEREDSELVDVSLADLGAAVGLTAAPV
                     AFEVTRWTAGLPQYPVGHLARVARIRDEIAKVPGLRVCGAVYDGVGIPACIASGQRAAD
                     EILATPTLARGTDGEAGQ"
     gene            2189..2902
                     /gene="hemQ"
                     /locus_tag="OGH68_RS29185"
                     /old_locus_tag="OGH68_29185"
     CDS             2189..2902
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962204.1"
                     /locus_tag="OGH68_RS29185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29185"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_264248064.1"
                     /transl_table=11
                     /translation="MSAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRSGYAEE
                     VQELFDQLAAKDVTVRGTYDVSGLRADADVMIWWHAETADELQDAYNRFRRTRLGRTLE
                     PVWSNMALHRPAEFNKSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKDVADLVAGLA"
     gene            complement(2987..4588)
                     /locus_tag="OGH68_RS29190"
                     /old_locus_tag="OGH68_29190"
     CDS             complement(2987..4588)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (103-477). E-value:
                     6.4e-22. Score: 70.3. Matches aSDomain:
                     nrpspksdomains_OGH68_RS29190_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380016.1"
                     /locus_tag="OGH68_RS29190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29190"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_264248066.1"
                     /sec_met_domain="PF00561 (E-value: 3.1e-26, bitscore: 91.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTSAKYGAVAGLSSLVLTSVTVAPADGTTTTTAESRGTVVAAER
                     AAAAGIDFGRCPAVEKLPDSVRCGTVKVPLDYADPSGRQIELTVSRVPASGRKSERQGA
                     LVYNPGGPGASSMTFPLAAELPEWKRIAEAYDIVGYAPRGVGRSAPLSCQDPEDFLKAP
                     TQAPTHPSLSYKKERIAQARAYADGCAKRNGEALRHYTSLNNARDLDVLRAALGEQKLT
                     FVGASYGTYFGALYATMFPSHVRRMVFDSAVNPDPKQIWYRNNLDQSEAFESRWADFRA
                     WVARHHETYGLGTTAEEVAHSYEQARARLAQEPAGGTVGPGQLHAAFLGAGYYDDYWPL
                     RATALSEYLKGNPEPLIAQAAPDRKRAKAAENGNAVYTAVECNDAPWPEDWLTWNVDNS
                     LLAQRAPFETWDNVWMNLPCAFWSAPRQRPLEVATAPGELPPTLILAAERDAATPYTGA
                     LELQRRLAGSVLITEEDAGTHGIGGGRNTCVNDHLEDYLLRGETPVRRASCAPHPEPDP
                     VSLDRRAEQRKLPHTV"
     aSDomain        complement(3158..4279)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_OGH68_RS29190_Abhydrolase_1.1"
                     /evalue="6.40E-22"
                     /label="OGH68_RS29190_Abhydrolase_1.1"
                     /locus_tag="OGH68_RS29190"
                     /protein_end="477"
                     /protein_start="103"
                     /score="70.3"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASSMTFPLAAELPEWKRIAEAYDIVGYAPRGVGRS
                     APLSCQDPEDFLKAPTQAPTHPSLSYKKERIAQARAYADGCAKRNGEALRHYTSLNNAR
                     DLDVLRAALGEQKLTFVGASYGTYFGALYATMFPSHVRRMVFDSAVNPDPKQIWYRNNL
                     DQSEAFESRWADFRAWVARHHETYGLGTTAEEVAHSYEQARARLAQEPAGGTVGPGQLH
                     AAFLGAGYYDDYWPLRATALSEYLKGNPEPLIAQAAPDRKRAKAAENGNAVYTAVECND
                     APWPEDWLTWNVDNSLLAQRAPFETWDNVWMNLPCAFWSAPRQRPLEVATAPGELPPTL
                     ILAAERDAATPYTGALELQRRLAGSVLITEEDAG"
     misc_feature    4663..4683
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.71"
                     /tool="antismash"
     gene            complement(5001..6437)
                     /locus_tag="OGH68_RS29195"
                     /old_locus_tag="OGH68_29195"
     CDS             complement(5001..6437)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452208.1"
                     /locus_tag="OGH68_RS29195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29195"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_264248067.1"
                     /sec_met_domain="DUF692 (E-value: 9.8e-91, bitscore: 302.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPQIAETVERLPGLDWVEVVAENVCPGHLPPALLRL
                     RSRGVRVVPHGVSLGLGGADRPSPRRLADLAERAEALDAPLVTEHIAFVRAGGALTASP
                     VLEAGHLLPVPRTWDALEVLCENVRIAQDSLPVPLALENIAALISWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNRGENPSAALDELPVEAIAYVHVAGGIERDGVWHDTHA
                     HPVTQPVLDVLSALRARVDPPGVLLERDDDFPAGAELERELATIRSTLTAAGGALTAAR
                     CVSPTPPLPETGLLPPRPAAWGDPQPGCTAAPPEFEARGSGGRAPGSGRHEVGNRPAAG
                     APVPTRQRLALAQTALLSALVAGTPAPQGFDGRRLRVQSRALAAKRADVVAKIAPELPE
                     ILGTGYRPAFLAYAGNRPMHAGYRRDALEFAEHLLSTGGVADAEARRELTLWWRERAAS
                     RPPGRATRLVRAARAALAGR"
     gene            complement(6584..7372)
                     /locus_tag="OGH68_RS29200"
                     /old_locus_tag="OGH68_29200"
     CDS             complement(6584..7372)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005310492.1"
                     /locus_tag="OGH68_RS29200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29200"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_264248070.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVVTVGALAFPVWSYADRSGTGQDNLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPTEAIKAAGDHLVVGHTDLDERAR
                     NVAAQLGVELPNQPTEQQQGWLRELSAAQGEEYERKFAQILRTAHGKVFALIAEVRHTT
                     RNTLIRQLATDANQTVLDHITMLEQTGRVDFDQIANGAAATATASPSGPPPPDGNLPPS
                     PPAVTPTGDQSFTSRPTPQPGAPTAINTDRPAPQDPVPAG"
     misc_feature    complement(7365..7381)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.66"
                     /tool="antismash"
     gene            7731..8459
                     /locus_tag="OGH68_RS29205"
                     /old_locus_tag="OGH68_29205"
     CDS             7731..8459
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719993.1"
                     /locus_tag="OGH68_RS29205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29205"
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="WP_264248072.1"
                     /transl_table=11
                     /translation="MVTSGASDTDENTGARSATKAAARQLLAKLGAGGLSLDAVARSSG
                     LAVTDVEAVFPHRDDLLTALVIDAYNESGAAMEAADQAASDARASAGARLLAATRALRQ
                     WSFDNPAEFALIYGSPIPDYHAPQDTVPPASRTPAVLAGIVRSGLEAGELTPPRRAVPG
                     PPLLRPEAVELFGGLPEAPFSDIIERGIVLWSNLIGLLVFQVFSRTHDSVRDETAFFDY
                     AIAVAAESVGLVVPPGETSD"
     gene            8530..9684
                     /locus_tag="OGH68_RS29210"
                     /old_locus_tag="OGH68_29210"
     CDS             8530..9684
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536534.1"
                     /locus_tag="OGH68_RS29210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29210"
                     /product="MAB_1171c family putative transporter"
                     /protein_id="WP_264248075.1"
                     /transl_table=11
                     /translation="MNTTRTLLFVISALSSYAALGYRLSQVRRSWRDSAYRALVVTLVF
                     QCLTFTMGAVAMGGDSFLGVGNLAILLMHLSAVAFCVSAQIILLRWATADEVAIRRTRY
                     WVITGIALNVLLAALFLLADGPGRPASDFDGTDRPLVLTYLLFFVVSQAVPCVTIFRQC
                     GPYARMTDNASLRQALRLLSVAAVILFLYCTARAVNILTGAAGIDIGVWRVAASVFSAV
                     GIVVLSLSLTLPSWGPSAAKLLAWARSYRSYRALYPLWRDLYEASPDIVLEPPGTSVSD
                     LDYRLHRRVVEIRDGWRDLRPYIDRSTTNGAARPGSSASEEARQAFTEAAQIRQALHAK
                     RTGTVPDDNADAGDFEDRDTDNFTAEVAWLTQVSVAYSKLGDAS"
     gene            10012..10455
                     /locus_tag="OGH68_RS29215"
                     /old_locus_tag="OGH68_29215"
     CDS             10012..10455
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144017.1"
                     /locus_tag="OGH68_RS29215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29215"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_264248077.1"
                     /transl_table=11
                     /translation="MSETEDRPLLAVRLDDLFKTVRPKGRHWTNAEVAEELKRAHPELK
                     VGGVYLSQLRTGKRSNPSPDLLAALARFFGVSVAYFFDEEVAQSVLGEVAAIEALRQAG
                     VRSVAMRAAGMKKENLQAITAIMDQYRHMQGLPPVADPPSEAE"
     gene            10452..11033
                     /locus_tag="OGH68_RS29220"
                     /old_locus_tag="OGH68_29220"
     CDS             10452..11033
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144016.1"
                     /locus_tag="OGH68_RS29220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_29220"
                     /product="toxin"
                     /protein_id="WP_264248078.1"
                     /transl_table=11
                     /translation="MRGTRKERSAEQDRHSQLKRLRRTGAQRLAELELPEVADVAELCR
                     HLGEIRDRPITLVPMQMPASHPCGMWVAARDEDLVFYDANTTSAHQEHIILHELGHIIC
                     CHRGAGLLDEATARLLFPNLDPDLVRDMLLRATYDDVQEQEAEIIAYLLSQRVGGTGRQ
                     PGDAPPAEEAAGRADSGKNAMLSRIERTLI"
     misc_feature    complement(11223..11243)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.63"
                     /tool="antismash"
ORIGIN
        1 tccaggtcgg cctgacggct gctcagctcg ccttccagag tgacgacgtc ggtcagcttc
       61 gtggcccgct ccatcagctc ccgcaccctg gccacgctcg cccgctgcgt cgcgatccgg
      121 ctctccacgt ccacgacctg gtcggtgacg tccttggcgt ccgccttgcg ggccaacagc
      181 ttgccggcgc ccgccagttc gctcaggacg gagtcgtacc gctcctgggg gacgcgcagc
      241 acgacccggg aggtgacatg ggtgtcgtcg atccgttccg tggtctcgtt ctccacgtgg
      301 ccgccggcgt tcagggccgt cgtacgggcg gcggcgagcg ccttcgtggc gtccttgacc
      361 tccacctcca gcgtggcggt ccggatgacg tgcgcctgca ccgccgcggc gggcttcgcg
      421 gggcggcggt cggcgccgcc ctccgccggc tgcccggcgg cctcgtccgc cgcgtaaccg
      481 gagcccgccg ccccgtccct ctcctgctgc gcggcgggct tcgccgcgct ccgcgcgctg
      541 tcgccggagt cgcccgcgcc gcaccccgcg agggcgagcg aggcggtgag aagcagcacc
      601 gcggatgtac gtcgtgaacc cataccggcc ccccggggga tgtgtggcga caagtcgcag
      661 gttcgacgta cgacccgcct ccgcgggttg ccggaacaag gtcccgaacc ggtcacggtc
      721 aggactcgga acggcgtttg agagagttgg accatgaacg tggacacacc tgcgccccat
      781 gtggtggtcg tcgggggcgg catcgccggt ctcgccgccg cgcaccggct gctcgcctcc
      841 ggcgtgcgcg tcaccctcct cgaggccacc gaccgggtcg gcggcaagct tcggtcgggg
      901 gagatcgagg gcgtacgcgt ggacctcggc gccgagtcga tgctcgcccg ccgcaccgag
      961 ggcaccggcc tggcccgcgc cgtcggcctc ggcgaccgcc tcgaaccgcc ggcgaccgcg
     1021 acctccgcgg tgtggacccg cgacgccctg cggcccatgc cgaagggcca tgtgatgggc
     1081 gtcccgggcg acgcgtcggc gctggccggg ctgctgtcgc ccgaggggct cgcccgcatc
     1141 gaacacgaga aggacctccc gccgaccggg atcggcgacg acgtggccgt cggcgcgtac
     1201 gtcgcggagc gcctcggccg ggaggtcgtc gaccggctcg tggagccgct gctcggcggg
     1261 gtgtacgccg gggacgccta ccgcatctcg atgcgcgcgg ccgtgcccca gctgttcgag
     1321 gctgccaggt cccacggctc gctgctcgac ggcgtgcggg acatccaggc cagggccgcc
     1381 gccgggcagc agaccggcgg gccggtcttc atgggcctcg acggcggcat cggcaccctg
     1441 ccgggcgccg tcgccgacgc cgtgcgcgcg cagggcggcg acatccgcac cgaaacgccc
     1501 gtcctcgggc tcgcccgcgc aggcgccggc tggcgggtcc gcacggaccg ggagacgctc
     1561 gccgccgacg ccgtggtgct cgcgacgccc gcctggtccg cgtccgcgct cctcgccggc
     1621 gagtcaccgg ccgcggcggc cgagctgggg cacgtcgagt acgcctcgat ggcgctcgtg
     1681 accatggcgt tccggcgcgc cgacgtgacc gggatgccgg acggcagcgg tttcctcgtc
     1741 ccgcccgtcg acggccgcac catcaaggcg tccacgttct cgtcgaacaa atggggctgg
     1801 gtcgcccggg gcgcgggcga cctgttcgtg ctgcgcacct ccgtcggccg ctacggcgag
     1861 gagcaggccc tcgaacgcga ggacagcgag ctcgtcgatg tctccctggc ggacctcgga
     1921 gcggcggtcg gactcaccgc cgctccggtg gcctttgagg tcacccggtg gacggccggt
     1981 ctgccccagt acccggtcgg gcacctcgcc cgggtcgccc gcatccgtga cgagatcgcc
     2041 aaggtgccgg gcctgcgggt ctgtggcgcc gtgtacgacg gtgtcggcat ccccgcctgc
     2101 atcgcgagcg ggcagcgtgc cgccgacgag attctcgcca cgccgaccct ggcgcggggc
     2161 acggacggcg aggcgggaca atagccgtat gagtgcgcca gaaaagatcc cgaacgcagg
     2221 caagaaggcc aaggacctca acgaggtcat ccggtacacg ctgtggtccg tcttcaagct
     2281 gcgcgatgtc ctgccggagg accggtccgg ctacgccgag gaggtccagg agctgttcga
     2341 ccagctcgcc gccaaggacg tcaccgtgcg cggcacgtac gacgtctccg gcctgcgcgc
     2401 cgacgccgac gtgatgatct ggtggcacgc ggagaccgcg gacgaactgc aggacgcgta
     2461 caaccgcttc cgccgcaccc gcctcggccg cacactcgag ccggtctggt cgaacatggc
     2521 gctgcaccgc cccgccgagt tcaacaagtc gcacatcccg gcgttcctgg ccgacgagac
     2581 gccccgcaac tacgtctccg tctacccctt cgtgcgctcc tacgactggt acctgctgcc
     2641 cgacgaggac cgccgccgga tgctcgccga ccacggcaag atggcccgcg gctacccgga
     2701 cgtgcgcgcc aacacggtcg cgtcgttctc actcggcgac tacgagtgga tcctggcctt
     2761 cgaggcggac gagctgtacc gcatcgtcga cctgatgcgt cacctgcgtg cctccgaggc
     2821 ccggatgcac gtccgcgaag aggttccgtt ctacaccggc cgccgcaagg atgtggcgga
     2881 cctggtggcc gggctcgcgt agcgtctcct cggcccctca agaacctgac ggccgggttt
     2941 ccgcaccacc ggtgcggata cccagccgtc catgtgaccc ggaagatcag acggtgtgcg
     3001 gcagcttccg ctgctccgcc cgccggtcca gcgacaccgg gtccggctcc gggtgcggcg
     3061 cgcatgatgc gcgccgcacc ggcgtctcac cccgcagcag gtagtcctcc agatggtcgt
     3121 tgacgcaggt gttgcggccg ccgccgatac cgtgcgtgcc cgcgtcctcc tccgtgatca
     3181 gcacggaacc cgccagccgc cgctgcagct ccagcgcccc cgtgtacggg gtggccgcgt
     3241 cccgttccgc ggccaggatc agcgtgggcg gcagctctcc cggcgcggtg gcgacctcca
     3301 gcggccgctg ccgcggcgcg gaccagaacg cgcacggcag gttcatccac acgttgtccc
     3361 acgtctcgaa cggcgcgcgc tgcgccagca gcgagttgtc gacgttccag gtgagccagt
     3421 cctcaggcca cggggcgtcg ttgcactcga ccgccgtgta gacggcgttg ccgttctccg
     3481 cggccttcgc cctcttccgg tcgggcgccg cctgggcgat cagcggctcc gggttgccct
     3541 tcagatactc cgacagcgcg gtggcgcgca gcggccagta gtcgtcgtag tagccggctc
     3601 cgaggaaggc cgcgtgcagc tggccggggc cgaccgtgcc gccggcgggc tcctgggcga
     3661 gccgggcgcg cgcctgttcg tagctgtgcg ccacctcctc ggccgtcgta cccaggccgt
     3721 acgtctcgtg gtgacgggcc acccaggcgc ggaagtcggc ccacctgctc tcgaatgcct
     3781 ccgactggtc caggttgttg cggtaccaga tctgcttcgg gtccgggttg accgcggagt
     3841 cgaagaccat acggcgcacg tgcgacggga acatcgtcgc gtacagggcg ccgaagtacg
     3901 tcccgtacga ggcgcccacg aaggtcagct tctgctcgcc gagcgccgcc cgcaggacat
     3961 ccaggtcgcg ggcgttgttc agcgacgtgt agtggcgcag cgcctcgccg ttgcgcttcg
     4021 cgcagccgtc cgcgtacgcc cgcgcctgtg cgatgcgctc cttcttgtac gagagggacg
     4081 ggtgggtcgg ggcctgtgtg ggcgccttga ggaagtcctc cgggtcctgg cacgacagcg
     4141 gcgccgaccg gccgacaccg cggggcgcgt agcccacgat gtcgtacgcc tcggcgatcc
     4201 gcttccactc gggcagctcg gccgccagcg ggaaggtcat gctggaggcg ccggggccgc
     4261 cggggttgta gacgagtgcg ccctgccgct cggacttcct accgctcgcg ggaacgcggc
     4321 tgacggtcag ttcaatctga cgccccgacg ggtcggcgta gtcgagcggg accttgacgg
     4381 tgccgcagcg gaccgaatcg ggcagcttct cgacggcggg gcagcggccg aagtcgatgc
     4441 cggccgcggc ggcgcgctcg gcggccacca cggtgccgcg ggactcggcg gtggtggtgg
     4501 tcgttccgtc tgcgggggca acggtgaccg aggtcaggac caaggaactg aggcccgcca
     4561 cggccccgta cttcgcggat gttcgcatcg cgttcccttc gtgcgcgggc ggccgggcga
     4621 cggccacggg acgaaaggga tcttcggcag gcgcggttca cgaagtaaag caccggctcg
     4681 gcggtttcgg ggtgattgac cccgatgccg gtcgctcatg acccgccgcc gcggaagccg
     4741 gagccggagc cgggggacga gccggcggcc ggcccccccg ggccgccggc cgcagtacgc
     4801 acgagccccc gggccccggc gagcgcagcg tggacgaccc tgggtagggc cgtccgcgag
     4861 gaaggccgcc tcgcggacgt cgcgcacgtg cgccgcggtg gagcgggcgg cgggccgggg
     4921 cggacctcga cgatccgcaa ggacggacgg gacgccgacg gtgagaggca cggcgtcgca
     4981 cgcgagcagc gtcgccccgc tcacctcccc gcgagcgcgg cgcgtgccgc cctcaccagc
     5041 cgggtcgccc ggcccggcgg ccgtgaggct gcccgctccc gccaccagag cgtcagctcg
     5101 cggcgggcct cggcgtccgc gacgcccccg gtcgacagca ggtgctcggc gaactcgagg
     5161 gcgtcgcgcc ggtagccggc gtgcatggga cggttcccgg cgtacgcgag gaacgccgga
     5221 cggtagcccg tgccgaggat ctccggcagc tcgggcgcga tcttcgcgac gacgtccgcc
     5281 cgcttggcgg cgagcgcgcg gctctgtacg cgcaggcggc gtccgtcgaa gccctggggg
     5341 gcgggggtgc ccgcgacgag cgcggacagc agcgcggtct gcgcgagcgc gaggcgctgg
     5401 cgggtgggta cgggtgcgcc tgcggcgggc ctgttcccca cctcgtgcct tcccgacccg
     5461 ggagctctgc ccccggaccc ccgcgcctca aactccggcg gggctgcagt gcagcccggc
     5521 tgggggtccc cccacgccgc cgggcgtggg gggaggaggc cggtttcggg aaggggcggg
     5581 gtgggggaga cgcatcgcgc agcggtcagg gcaccacccg ccgccgtcag cgtcgaccgg
     5641 atcgtggcca gttcacgctc cagctccgcc ccggccggaa aatcgtcgtc gcgctccagc
     5701 agcacccccg gcgggtcaac cctcgcgcgg agggccgaca acacgtccag caccggctgt
     5761 gtcaccgggt gcgcgtgcgt gtcgtgccag acgccgtcgc gttcgatgcc gcccgccaca
     5821 tggacgtagg cgatcgcctc gaccgggagt tcgtccagcg cggccgacgg gttctccccg
     5881 cggttgacgt ggttggtgtg caggttggcc acgtcgatca gcagccgtac gccggtccgt
     5941 tcgacgagtt ccgcgaggaa ctggccctcg gtgagctcct cgtccggcca ggagatcaac
     6001 gccgcgatgt tttccagcgc cagcggcacc ggcagcgagt cctgtgcgat gcgcacgttc
     6061 tcgcacagca cctccagcgc gtcccaggtg cgggggacgg gcagcagatg gcccgcctcc
     6121 agcacggggg atgcggtcag tgcgcctcct gcccgtacga acgcgatgtg ttcggtgacc
     6181 agcggggcgt cgagtgcttc cgccctctcg gccagatccg ccagccgccg cggcgagggc
     6241 cggtccgcgc cgccgagacc gagggagacg ccgtgcggga cgacgcggac gccgcgtgag
     6301 cgcagccgga gcagcgcggg cggcagatgg ccggggcaga cgttctccgc gacgacctcc
     6361 acccagtcga gtcccggcag ccgctccacc gtttccgcga tctgaggccg ccagccgatc
     6421 ccgatcccga gttccatggt ccccctcctc cgctcacgcc cgacgtggga cggtgctggg
     6481 gtcatggccc cgtcacggcg aggtgaatcc ggaggagggg acgttcagag gttcatttga
     6541 ggttccggcc cgcccgaaaa gcaggccgcc gggaccgtgc ccgtcagccc gccgggacgg
     6601 ggtcctgggg cgccgggcgg tcggtgttga tcgcggtggg cgcacccggc tgcggggtgg
     6661 gacgggacgt gaacgactgg tcgcccgtcg gtgtgaccgc aggcggcgac gggggcaggt
     6721 tgccgtccgg cggcggcggg ccgctcgggc tggccgtcgc ggtcgcggcg gcaccgttgg
     6781 cgatctggtc gaagtcgacg cggccggtct gctccagcat ggtgatgtgg tcgagcaccg
     6841 tctggttggc gtccgtggcg agctgccgga tcagcgtgtt gcgggtggtg tgcctgacct
     6901 cggcgatcag cgcgaacacc ttgccgtgcg cggtccgcag gatctgcgcg aacttgcgct
     6961 cgtactcctc gccctgtgcc gcggacagct cccgcagcca gccctgctgc tgctcggtgg
     7021 gctggttggg cagctcgacg ccgagttgcg cggcgacgtt gcgcgcccgc tcgtccaggt
     7081 ccgtgtggcc gacgacgagg tggtcgccgg ctgccttgat ggcctccgtg ggcgcgcgtt
     7141 cgatcgcctg ctgcccggcg ggcagctccc acagtccggc cagccgtacc ttgaccaaga
     7201 agtcgcggtc ggtggccgac agcggccccc actgggttgc cacgctcgag gcgttgagat
     7261 tgtcctgacc ggtgccggaa cggtcggcgt aggaccacac ggggaaggcg agtgcgccga
     7321 ccgtgacgac cagggccgcg atgatgagtg ctgttccgtt gatgcgccgc aacaatgtgc
     7381 ctccagagcc gatgcgttac acggcaacca atcgccctgc tgaacggccg gtcagcccgc
     7441 cggcgacagc cggttcggct cgccggcccg gccgccggtc gaacttagcc ggtcaaacta
     7501 ctgggcgata ccggggagta cgcaaccggg gcccggaacg ttcaacacgg cggatcgtgg
     7561 tacccctacc cgccggtgga ccgtcaaccg cagccggatg gcgaccgttc cgtcgaccgg
     7621 agggcaccgt ttccacggtc gtacggtcga gtgtccggat cacagcgcag ggccgaggct
     7681 ctcgggcgcc tgcctcacgg tcgttgtgcg tcggcaggta cggtctggtc atggttactt
     7741 ccggagcaag cgacaccgat gagaacaccg gggcgcggtc ggccaccaag gccgcggcgc
     7801 ggcaactgct cgcgaaactg ggggccgggg gactgtccct ggacgccgtt gcccggagca
     7861 gcggtcttgc cgtcaccgat gtggaagccg tcttccctca tcgggacgac ctgctgaccg
     7921 cgctggtcat cgacgcctac aacgagtccg gtgccgcgat ggaggcggcc gatcaggccg
     7981 cctcggacgc ccgggcatcg gcgggcgcac ggctcctcgc ggctactcgc gccctgcggc
     8041 agtggtcctt cgacaacccc gccgagttcg cactcatcta cggctcgccc attccggatt
     8101 accacgcccc gcaggacacg gtcccgcccg cctcgcgcac ccccgcggtc cttgccggca
     8161 ttgtgcgttc gggtctggag gccggggaac tcacgccacc gcggcgggcg gtgcccggac
     8221 ctccgttgct gcggcccgag gccgtggagc tcttcggcgg cctgcccgag gcaccattct
     8281 cggacatcat cgagcgcggc atcgtgctgt ggagcaatct gatcgggctc ctggtcttcc
     8341 aggtcttcag ccgtacgcac gacagcgtca gggacgagac cgcgttcttc gactacgcca
     8401 tcgccgtggc ggccgagagc gtcggactcg tggttccccc gggcgagacc tccgactgat
     8461 ccatccaccg ttgaactgcc tttcgctgaa ccgtcttacg cgtgcgcggc tcctgcaggg
     8521 aatcaggctg tgaacaccac tagaaccctc ctgttcgtca tctcggcgct ctcctcgtac
     8581 gccgcgctcg gctaccggct gtcccaagtc cggcgcagct ggcgggacag cgcctaccgc
     8641 gcactggtcg tcacgctggt gttccagtgc ctcacgttca cgatgggggc ggtggccatg
     8701 ggaggcgaca gcttcctggg cgtcggcaac ctcgccatcc tgctgatgca cctgtcggcg
     8761 gtcgccttct gcgtcagcgc gcaaatcatc ctgctgcgct gggccaccgc cgacgaggtg
     8821 gcgatacgca ggacccgcta ctgggtgatc accggcatcg ccctgaacgt actccttgcg
     8881 gctctgttcc ttctcgccga cggtcccggc cggccggctt cggacttcga cggcacggac
     8941 cggccgctgg tcctcaccta cctgctgttc ttcgtcgtgt cccaggcggt tccgtgcgtc
     9001 accatcttcc gccagtgcgg cccctacgcc cgtatgaccg acaacgcctc gctgcgccag
     9061 gccctgcggc tgctgtccgt cgccgcggtg atcctcttcc tctactgcac ggccagggcg
     9121 gtcaacatcc tcaccggtgc ggcagggatc gacatcggag tctggcgggt cgccgcttcc
     9181 gtcttcagtg cggtgggcat cgtcgttctc tctctcagcc tgaccctgcc ctcgtggggc
     9241 ccttcggcgg ccaagctcct ggcatgggcg cgtagttacc ggtcgtaccg ggcgctgtac
     9301 ccgctctggc gggacctgta cgaggcctcc ccggacatcg tcctggagcc gcccgggact
     9361 tcggtctccg acctcgacta ccgcctgcac cggcgggtcg tcgaaatacg tgacggatgg
     9421 cgggacctgc gcccctatat cgaccgcagc accaccaacg gtgctgcccg accgggctcg
     9481 agtgcgagcg aggaggcccg tcaggcgttc accgaagcag cgcagatcag acaggcgctg
     9541 cacgccaagc gaaccggcac cgtgcccgac gacaacgcgg acgccggcga cttcgaagac
     9601 cgcgatacgg acaacttcac cgcggaagta gcctggctca cccaagtctc ggtcgcctac
     9661 agtaagctcg gcgacgcgag ttgagagctc ccgcccggtc cgcatccccc gtcggaccgg
     9721 gcgggcttgt tcccccgatt tcccccgttc ccccgttccc cgtttctcct cggccggggc
     9781 gggcgcgcct ccaccccggc cggcctggcc caccacgttc ccgagagccg gaccgacatc
     9841 gccgaccggc cggccgccaa cttgaccacc cccgtaagcc aagttagctg acagctatcg
     9901 gcggttccca acgttggcgg actctcggtc aattgtcaac tgctcgctaa tctgcgaaac
     9961 ttggaaatag ccgagggcta tgccgaggag gtgacgggag ggagattcac catgtccgag
    10021 accgaggacc ggccgctgct tgcggtgcgt ctggacgacc tcttcaagac ggttcgcccg
    10081 aagggccggc actggaccaa tgccgaggtg gcggaggagc tgaagcgggc acaccccgaa
    10141 ctcaaggtcg gtggtgtgta tctgtcgcag ttgcggacgg gcaagcgctc caacccttcc
    10201 cccgacctac tggctgccct ggcccgcttc ttcggtgtgt cggttgccta cttcttcgac
    10261 gaagaggtcg cccagtcggt gctcggcgag gtcgccgcca tcgaggcact gcggcaggcc
    10321 ggggtccgtt ccgtggccat gcgcgcggcc gggatgaaga aggagaacct ccaggccatc
    10381 acggccatca tggatcagta ccggcacatg cagggtcttc cgcccgtcgc cgaccccccg
    10441 tcggaggcgg agtgagaggt acgaggaagg aacggtcggc cgaacaggac cgccacagtc
    10501 agctcaagag gctccgcagg accggcgcgc agcgactcgc cgaactcgag ctgccggagg
    10561 tggccgatgt ggccgagctg tgccgccatc tcggagagat ccgcgaccgc ccgatcacgt
    10621 tggtcccgat gcagatgccc gcgtcgcatc cgtgcgggat gtgggtcgcc gcacgcgacg
    10681 aggacctcgt cttctacgac gccaacacga ccagcgcgca tcaggagcac atcatcttgc
    10741 atgagctggg ccacatcatc tgctgtcatc ggggcgcggg cctgctggac gaggcgaccg
    10801 cccggctcct cttccccaac ctcgaccccg acctcgtacg cgacatgctc ctgcgcgcga
    10861 cctacgacga cgttcaggaa caggaggcgg agatcatcgc ctatctgctc tcccaacgag
    10921 tgggcggcac cgggcgacag cccggcgacg ctccccctgc cgaggaagcg gcgggccgcg
    10981 cggactccgg aaagaacgcc atgctcagcc ggatcgaacg caccctgatc tgacgtgagt
    11041 caattactcc gtggcggatg ccacctcctc ggccaggatc gacacggtcc tgcggatgtc
    11101 gtcctcgccg tgttcgctgg tgacgaagaa ccgcagccgc gcgaggccct cctccacggc
    11161 gggatggaag atcggatctg cgatgacgcc ccggtcgaaa agccggtccg cgacacgcaa
    11221 ggtcttcgtc gagctgccga gaaggcacgg aacgatcggt gtgtgggcac tggatcccgt
    11281 cggcagaccg gccgaggcgg caaggccgag gaagagctcc gcgttgcgcc gcagagcacg
    11341 cacccggtgc ggttccgcgg cgatcagctc ggcggcggcc agcgccgcgg ccgcgttggc
    11401 cggggtcagg ccgacgctgt agacgaagcc cggcagt
//
