LOCUS       NZ_CP107567            10975 bp    DNA     linear   CON 18-NOV-2023
DEFINITION  Streptomyces peucetius strain NA0869 chromosome, complete genome.
ACCESSION   NZ_CP107567
VERSION     NZ_CP107567.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces peucetius
  ORGANISM  Streptomyces peucetius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8050611)
  AUTHORS   Zhao,E. and Ge,H.
  TITLE     Cytochrome P450 Catalyzes Benzene Ring Formation in the Biosynthesis
            of Trialkyl-Substituted Aromatic Polyketides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8050611)
  AUTHORS   Zhao,E. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) School of Life Sciences, Nanjing University,
            No. 163 of Xianlin Avenue, Qixia District, Nanjing, Jiangsu 210033,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: unicycler(0.4.8) v. v. 3
            Assembly Name                     :: NJU_NA0869
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/18/2023 06:49:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,216
            CDSs (total)                      :: 7,126
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 144 of 303
            Pseudo Genes (incomplete)         :: 227 of 303
            Pseudo Genes (internal stop)      :: 22 of 303
            Pseudo Genes (multiple problems)  :: 85 of 303
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:23:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3279359
            Orig. end                         :: 3290334
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107567.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from IFB-NA0869, Nanjing
            University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10975
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="10"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10975
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10975
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5975](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5975
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    20..34
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
     misc_feature    437..451
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     misc_feature    complement(553..568)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.19"
                     /tool="antismash"
     misc_feature    666..680
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.33"
                     /tool="antismash"
     gene            complement(831..1628)
                     /locus_tag="OGH68_RS14865"
                     /old_locus_tag="OGH68_14865"
     CDS             complement(831..1628)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134922.1"
                     /locus_tag="OGH68_RS14865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14865"
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="WP_264250089.1"
                     /transl_table=11
                     /translation="MRAALVQIEVRPDEPADARRKRVADLVRAHDGADLVVLPELWPVG
                     AFAFDAFAEHAEPLDGPTAAAMSSAASDAGVWLHAGSVVERGPDGTLHNTSLLFAPDGR
                     LHRTYRKIHRFGFDKGEAALMARGDEIVTAPLDGLGVTVGLATCYDLRFPEQFRLLVDA
                     GAEFLVVPAGWPAARRAHWSLLARARAVESQAYVLACGTAGTHAGIEQAGHSVVVDPWG
                     EVVAEAGPGEETLLVDLDPGLVAGTRADFPALRDRVLGLPLPR"
     gene            complement(1677..2438)
                     /locus_tag="OGH68_RS14870"
                     /old_locus_tag="OGH68_14870"
     CDS             complement(1677..2438)
                     /NRPS_PKS="Domain: PKS_KR (6-183). E-value: 4.6e-13. Score:
                     41.4. Matches aSDomain:
                     nrpspksdomains_OGH68_RS14870_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539482.1"
                     /locus_tag="OGH68_RS14870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14870"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_264244332.1"
                     /sec_met_domain="adh_short (E-value: 3.3e-33, bitscore:
                     113.9, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRRHALVTGASRGIGAAVAERLAAAGHVVAVHCRADVAAAKAVA
                     AALPGTGHTVVTGDIGDPDQARAVLDTAVDELGAVDVLVNNAGIYTEQPIATTSYADWR
                     ADWQRVVDVNLLGTANLTWCLVDHLLRRVQGPQGARIINVGSRGAHRGEPEAPAYGASK
                     AAVHALTQSLAQSLGPRGIAVTAVAPGFVLTDLTEPMLDGPTTEAVRAQSPFGRIAEPA
                     DIADAVAWLASPGAEWASGAVLDLNGASHLR"
     aSDomain        complement(1890..2420)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_OGH68_RS14870_PKS_KR.1"
                     /evalue="4.60E-13"
                     /label="OGH68_RS14870_PKS_KR.1"
                     /locus_tag="OGH68_RS14870"
                     /protein_end="183"
                     /protein_start="6"
                     /score="41.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="LVTGASRGIGAAVAERLAAAGHVVAVHCRADVAAAKAVAAALPGT
                     GHTVVTGDIGDPDQARAVLDTAVDELGAVDVLVNNAGIYTEQPIATTSYADWRADWQRV
                     VDVNLLGTANLTWCLVDHLLRRVQGPQGARIINVGSRGAHRGEPEAPAYGASKAAVHAL
                     TQSLAQSLGPRGIA"
     CDS_motif       complement(2355..2420)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_OGH68_RS14870_0001"
                     /evalue="1.30E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="OGH68_RS14870"
                     /protein_end="28"
                     /protein_start="6"
                     /score="14.6"
                     /tool="antismash"
                     /translation="LVTGASRGIGAAVAERLAAAGH"
     gene            complement(2453..3088)
                     /locus_tag="OGH68_RS14875"
                     /old_locus_tag="OGH68_14875"
     CDS             complement(2453..3088)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.3"
                     /locus_tag="OGH68_RS14875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14875"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_264244334.1"
                     /transl_table=11
                     /translation="MRQTRAEHTRHALITAAATEFDQQGYAGTSLAAVTKAAGVTMGAL
                     TFHFRTKADLAVAVCAEGETVTREALDRMIAELAPDLRSVVELCLETIRLLGAEVSVRA
                     AARLAQERATAHERWHSVWTAALRDLLKRIRAADGLKGDAEDLELLAVYLMAGAEAFIR
                     AGHPAGSVQKQLGCLWTLTLDGFPGAPRQPLCAPDRGPSTRTCRDLLT"
     gene            complement(3180..4232)
                     /locus_tag="OGH68_RS14880"
                     /old_locus_tag="OGH68_14880"
     CDS             complement(3180..4232)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (7-168). E-value: 5e-11.
                     Score: 34.1. Matches aSDomain:
                     nrpspksdomains_OGH68_RS14880_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133276.1"
                     /locus_tag="OGH68_RS14880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14880"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_264244336.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5.5e-11, bitscore:
                     40.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRTRVVVTGGTGFVGRAVLRDLLRDGASGRGVSVRAAVRTVRPG
                     TEDVPDLERVTAELTDPRSVRGLLDGADVLVHLASRVVGSEEECTAVNVDGTRAVMAEA
                     RRHSVGRIVHLSTAAVYGAGPHRGIPVDGVTPDPVSPASRTRLAGERVALDAGAVVLRP
                     GLVLGAGDQWVVPALAEFLQRVPAAWDGGRGQASLVDVGDLARLIGTLARSPDPLPHGV
                     HHASHPRPVRNRDLLACLAELDVLPAGSGDLPWAECLRRLRRTPGRLSERQFTLVAGDH
                     WYLSEEIWRLTGCPEGPGPLARLADAAPWYRAHLAGRPGARPRTVPHGEQDAKAVPDMG
                     RPDACRSGRG"
     aSDomain        complement(3729..4211)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_OGH68_RS14880_NAD_binding_4.1"
                     /evalue="5.00E-11"
                     /label="OGH68_RS14880_NAD_binding_4.1"
                     /locus_tag="OGH68_RS14880"
                     /protein_end="168"
                     /protein_start="7"
                     /score="34.1"
                     /tool="antismash"
                     /translation="VTGGTGFVGRAVLRDLLRDGASGRGVSVRAAVRTVRPGTEDVPDL
                     ERVTAELTDPRSVRGLLDGADVLVHLASRVVGSEEECTAVNVDGTRAVMAEARRHSVGR
                     IVHLSTAAVYGAGPHRGIPVDGVTPDPVSPASRTRLAGERVALDAGAVVLRPGLVLG"
     CDS_motif       complement(4149..4214)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_OGH68_RS14880_0001"
                     /evalue="6.10E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="OGH68_RS14880"
                     /protein_end="28"
                     /protein_start="6"
                     /score="12.5"
                     /tool="antismash"
                     /translation="VVTGGTGFVGRAVLRDLLRDGA"
     gene            complement(4229..4864)
                     /locus_tag="OGH68_RS14885"
                     /old_locus_tag="OGH68_14885"
     CDS             complement(4229..4864)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264896.1"
                     /locus_tag="OGH68_RS14885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14885"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_264244339.1"
                     /transl_table=11
                     /translation="MARPRQERAERTREALLRAAAETFDESGYAGAGLNRILARAGVTT
                     GAMYFHFKSKEDLARAVIVEQAAELDFPRDQSGLQQLLDMCQYLAVEMQNNVLFRAGVR
                     LAVEQNEAELLDYSIYDWWADQFREQLVKAREKGQLRPDVDEVAFSQVLVGAYTGTQIM
                     ARLSSGRVDLPERISTMWRCLLPAIAPPEVIAGLNLDPRPAGEETDAS"
     gene            5001..5975
                     /locus_tag="OGH68_RS14890"
                     /old_locus_tag="OGH68_14890"
     CDS             5001..5975
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010644108.1"
                     /locus_tag="OGH68_RS14890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14890"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_264244341.1"
                     /sec_met_domain="AfsA (E-value: 5.2e-22, bitscore: 76.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVTQGHETITLLPVSQEYTHKTNTGEVLLRSWQPVADDTFTVTA
                     EWPDTHSFYAPRNGLHDPLQLSETIRQTLPLLSHGAYGVPFGHQLLWQDFSWEIDREAS
                     RSDGSPAELELRIRCFDVTYRKDRASAMSLNIEAERSGRHLATARTRFTIQDPTVYRRL
                     RGRYADKAAANAGAVPLPPPAAMSPFGRDSFADVVLASTDLPHRWQLRADTAHPILFDH
                     PVDHAPGMLLLEAARQAALATGHPEQRVVLGMASVFSRYAELDSPCWITTEPIAAGDEQ
                     RRGEWSAQGDRTANRVRVSAEQNDREIFSSEVTLAPLHPAPLA"
     misc_feature    complement(5008..5021)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.68"
                     /tool="antismash"
     gene            complement(6770..7129)
                     /locus_tag="OGH68_RS14895"
                     /old_locus_tag="OGH68_14895"
     CDS             complement(6770..7129)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563909.1"
                     /locus_tag="OGH68_RS14895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14895"
                     /product="DUF805 domain-containing protein"
                     /protein_id="WP_264244344.1"
                     /transl_table=11
                     /translation="MHWYIDVLKKYAAFSGRARRQEYWMFTLISAIIYIALMVIDLSLL
                     GTGVIAGIYELAVLLPSLAVGVRRLHDTGRSGWWLLIALVPLVGAIVLLVFLASDGKPE
                     ENEHGPNPKLAAAAA"
     misc_feature    complement(7211..7226)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.16"
                     /tool="antismash"
     misc_feature    7213..7224
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.75"
                     /tool="antismash"
     gene            complement(7697..8368)
                     /locus_tag="OGH68_RS14900"
                     /old_locus_tag="OGH68_14900"
     CDS             complement(7697..8368)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055223.1"
                     /locus_tag="OGH68_RS14900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14900"
                     /product="YdcF family protein"
                     /protein_id="WP_264244346.1"
                     /transl_table=11
                     /translation="MTLSDAVMKDAQHLWDYQLMGHAPRPCSVVIGLGSHDLGVADVTA
                     DLYRQGMAPVVVFTGATSRTTRERMPRGEAEHYRERAIELGVPDDAILVEPRARNTGEN
                     IRFSRALLEEQGVPVSSVLLVSKPYEERRAYATARKLWPEVEFVSASAPMPLADYVRSI
                     GDARLVVDMLVGALQRLLIYPAQGFMISQDVPQDVQDAYERLQAQGFTSRLIPDATGRS
                     E"
     gene            complement(8365..9156)
                     /locus_tag="OGH68_RS14905"
                     /old_locus_tag="OGH68_14905"
     CDS             complement(8365..9156)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892546.1"
                     /locus_tag="OGH68_RS14905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14905"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_264244348.1"
                     /transl_table=11
                     /translation="MAPKWRELADRLAEEIRSGERPPGQQLPHIRELVEKGEGSKSTVH
                     AAYKALEAEGLVTSSRGHGTVVREQAPLKRLGIGRYDKAKWRDGDEVAFIADRVASGRA
                     FRRNEQTQTVSRVPAPPKVAEALGVPADSEVYARARLVKEGEQPTHSLTSYYRPEHVEG
                     TRLVDPTPGPAGRGGGYRVLYDAGYEIDHMREALFARVPTAEEAKLLQLPPGEPVMELH
                     RTTYTADGTVVEFAVGIHAASRFAWEYDFKVPDSAQPKEGS"
     gene            9306..9644
                     /locus_tag="OGH68_RS14910"
                     /old_locus_tag="OGH68_14910"
     CDS             9306..9644
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892246.1"
                     /locus_tag="OGH68_RS14910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14910"
                     /product="hypothetical protein"
                     /protein_id="WP_264244349.1"
                     /transl_table=11
                     /translation="MSSFKIDTSTAVVFVAVEPKAKVINKQTGEIAVDRETGAKMMTVG
                     LTIADEGEANLYTVSIPETGVPEGITLGMPVKVVGLKARDWENTFNGEKRFGIAFRAVA
                     LVVPSVKG"
ORIGIN
        1 agcccggccg ccgccgcggc gatctcgtcg gccatgcggc cggcggccgg gtgcgccacc
       61 agccagtcct tgtgcccggt gaggaacgcc gtcatggcgg tgacggtgcg gcgcggcggg
      121 gtggtggacg gccgctcctc cgccaccagc cgggcccacg aggagaggcg ggccaccgcg
      181 tcgtggcggg agtccacggc cttggtgctc accggcaggg tgttctccct gctgccggtg
      241 acgcgcgggg cgagcccgcc ttcggcgggc gtcaggtact gctcgctctc gcggtgcagt
      301 gcgggcagcg ccgccaggtc gcgcaggaac gcgcggaggc aggacccgca gagccgggcg
      361 cccggctctg cgcggcggct cggcaggcca ccttcctggg cggccctgcg gcaacgggtc
      421 gatgtgcact gcgaggggat gcggtcggcc acggtctcat cacctttccg ggaggcaggc
      481 gggatgtccg agcggagtcg gacgacgggt ctgaggaggg gtctcacgac ggacccgagg
      541 acgggtccga ggtcgaacag gaggtcgccg cacggagatc gcacaactcg gcggcaacaa
      601 aaaagcgccg cacgaggcga cgcgacggca gttcggcggc tcctggaaaa gggcagactg
      661 cctcgcgaag aggcgttaca cagctgcatg gctcaccgca aggccttccc ggtggtcagt
      721 tttccctgac tgctcatcaa gtcacggtcg cgcaactgcg gttgactgcc cgcaagcaca
      781 gcaccgcccg cccgtgggac acgggcgggc ggcagtgaca gcacggcgaa ctaccggggc
      841 agcggcaggc cgaggacccg atcgcgcagg gcggggaagt cggcgcgggt gccggcgacg
      901 agcccggggt cgagatccac gagcagtgtc tcctcccccg gccccgcctc ggccaccacc
      961 tcgccccacg ggtcgacgac cacgctgtgc ccggcctgtt cgatcccggc gtgcgtaccg
     1021 gcggtgccac aggccagtac gtacgcctgc gactccaccg cacgtgcccg ggccagcagc
     1081 gaccagtggg cgcgccgtgc cgccggccag ccggccggga cgacgaggaa ctcggcgccg
     1141 gcgtcgacca gcagccggaa ctgctcgggg aagcgcaggt cgtagcaggt ggccaggccg
     1201 accgtgacac cgaggccgtc cagcggcgcg gtgacgatct cgtcgccccg tgccatcagc
     1261 gccgcctccc ccttgtcgaa gccgaaccgg tggatcttgc ggtaggtccg gtgcagccgc
     1321 ccgtccgggg cgaagaggag cgaggtgttg tggagcgtgc cgtccgggcc gcgttcgacg
     1381 acggatccgg cgtgcagcca gacgcccgcg tccgacgccg cggagctcat cgcggcagcc
     1441 gtcggaccgt cgagcggctc ggcgtgctcc gcgaaggcgt cgaaggcgaa ggccccgacc
     1501 ggccagagct cgggcagtac gacgaggtcg gcgccgtcgt gtgcccgtac gagatcggcg
     1561 acccgtttcc ggcgtgcgtc ggccggctcg tcgggccgta cctcgatctg gacgagggcg
     1621 gcgcgcagag tggtgttgtc catgggccgg ggccttcctt tcacggggcc ttcctttcag
     1681 cgcaggtggg aggcgccgtt gaggtcgagc acggcgccgc tcgcccattc ggccccgggg
     1741 gacgccagcc acgccacggc atcggcgatg tcggcgggtt cggcgatgcg cccgaacggg
     1801 ctctgcgccc ggacggcttc ggtggtggga ccgtcgagca tgggctcggt caggtccgtg
     1861 aggacgaatc ccggtgcgac ggcggtcacc gcgatcccgc gcgggccgag cgactgggcg
     1921 agggactgtg tcagggcgtg gacggctgcc ttgctcgcgc cgtacgcggg ggcctcgggc
     1981 tcgccccggt gggcgccgcg cgagccgacg ttgatgattc gcgcgccctg cgggccctgg
     2041 acacggcgca ggaggtggtc gaccaggcac caggtgaggt tggcggtgcc gaggaggttg
     2101 acgtcgacga cccgctgcca gtcggcccgc cagtcggcgt aggacgtggt ggcgatgggc
     2161 tgttcggtgt agatgcccgc gttgttgacc agtacgtcca cggcaccgag ttcgtcgacg
     2221 gcggtgtcga ggacggcccg ggcctggtcc ggatcgccga tgtcgccggt caccacggtg
     2281 tgaccggtgc cgggcagggc cgcggccacg gcctttgcgg cggcgacatc ggcacggcag
     2341 tgcacggcca ccacatgccc ggcggcggcc agccgctccg cgacggcggc tcctataccg
     2401 cgcgatgcgc cggtgacgag cgcgtggcgt cgaatcatgt gcggtgtctc cttcaggtca
     2461 gcaggtctcg gcacgtccgt gtggacggcc cgcggtcggg tgcgcacagg ggctgccggg
     2521 gagcgccggg aaacccgtcg agcgtcaggg tccacagaca gccgagctgt ttctgtacgc
     2581 tgccggccgg gtgaccggcc cggatgaacg cctcggcccc ggccatcaga tacacggcga
     2641 gcagttcgag gtcctcggca tcgcccttca ggccgtccgc ggcgcggatc cgcttcagca
     2701 ggtcccgcag tgcggcggtc cacaccgagt gccagcgttc gtgtgccgtc gcccgttcct
     2761 gggcgagccg cgccgcggca cgcacactga cctccgcgcc cagcaaccgg atggtctcca
     2821 ggcagagttc gacgaccgac cggagatcgg gagcgagctc cgcgatcatc cggtccagtg
     2881 cttcgcgggt gacggtctcg ccttcggcgc agaccgccac cgccagatcc gccttggtac
     2941 ggaagtgaaa ggtcagtgct cccatcgtga ccccggcagc cttcgtgacc gcggccagcg
     3001 aggtcccggc gtacccctgc tggtcgaact cggtcgcggc tgcggtgatc agagcatgac
     3061 gtgtgtgctc ggcccttgtc tgtctcacca cggctcaccc tcggtatgca actgggctgg
     3121 tccggagggg ccgtcgtact gcagacctgg gccgcgcacc acaaccagtg gtcgggaaac
     3181 tacccccggc cggaccggca ggcgtccggg cggcccatat ccgggacggc tttagcatct
     3241 tgctcaccat gcgggacggt ccgcggacgc gcgccgggcc gtccggcgag gtgtgcgcgg
     3301 taccaggggg cggcgtcggc cagccgcgcc agcggccccg ggccttccgg gcagcccgtc
     3361 agccgccaga tctcctcgct gaggtaccag tggtcccctg ccacgagcgt gaactgacgt
     3421 tcgctcagcc gtcccggtgt cctgcgcagt ctgcgcaggc attccgccca cggcaggtct
     3481 ccgctgcccg ccggcagcac gtccagttcc gccaggcacg ccagcaggtc ccggttgcgt
     3541 acgggccggg gatgactggc gtggtgcacc ccgtgcggca gggggtccgg cgagcgtgcc
     3601 agggtcccga tcagccgggc caggtctccg acgtcgacga gcgacgcctg tccgcgaccg
     3661 ccgtcccagg cggccgggac gcgctggagg aactcggcca gcgccgggac cacccactgg
     3721 tccccggcgc cgaggaccaa cccgggccgc agcacgacgg cgcccgcgtc cagggcgacc
     3781 cgctcccccg ccagccttgt tctgctggcg ggggagaccg gatccggagt gaccccgtcg
     3841 acgggaatgc cgcgatgggg tccggcgccg tacaccgcgg cggtggacag gtggacgatc
     3901 cgtccgacgc tgtggcgccg ggcctccgcc atgaccgccc gggtgccgtc gacgttcacg
     3961 gccgtgcatt cctcttcgct gccgacgacg cgggaggcga gatggacgag gacgtcggcg
     4021 ccgtccagca gacccctcac cgagcggggg tccgtcagct ccgccgtcac ccgttccagg
     4081 tccggtacgt cctccgtgcc ggggcgcacg gtccggaccg cggcccggac cgacacgccg
     4141 cggccgctcg cgccgtcgcg cagcaggtcc cgcaggaccg cccggccgac gaacccggta
     4201 ccgcccgtca ccaccacccg cgtccggttc acgaggcgtc ggtctcctca ccggccgggc
     4261 gggggtccag gttcagcccg gcgatgacct cgggcggcgc tatggcgggc agcagacaac
     4321 gccacatcgt gctgatccgt tccgggaggt cgacacggcc cgaggagagc cgggccatga
     4381 tctgcgttcc ggtgtaggcg ccgacgagga cctgggagaa ggcgacttcg tccacgtcgg
     4441 gccgcaactg ccccttctcg cgggccttga ccagctgctc gcggaactgg tccgcccacc
     4501 agtcgtagat cgagtagtcg agcagctccg cctcgttctg ctccaccgcc agccggacac
     4561 cggcgcggaa aagcacgttg ttctgcatct cgacggccag gtactgacac atgtccagca
     4621 gttgctgcag accggactgg tcacgcggga agtcgagctc ggcggcctgc tccacgatga
     4681 ccgccctggc caggtcctcc ttcgacttga agtgaaagta catggcgcct gtggtgacgc
     4741 ctgcgcgcgc aaggatccgg ttcagacccg ccccggcgta gccgctttcg tcgaacgtct
     4801 ccgccgctgc gcgcaggagg gcttcgcgcg tgcgttccgc gcgctcctgt cgtggtcgcg
     4861 ccatgggctg cctccgggaa tctctcgaca aacaaaaaga acgacctcta tgttattcga
     4921 gcgacctcgc ttcgtcacac cgagggcccg cctctcggtc ccgcacgtca aaaccgcatt
     4981 ccattggggg agccatgcag ttgagcgtca cacagggtca cgaaaccatc actctcctgc
     5041 ccgtctcgca ggagtacacg cacaagacga acaccgggga agtactcctg cggagctggc
     5101 agccggtcgc cgacgacacc ttcaccgtca ccgccgaatg gcccgacacc cacagtttct
     5161 acgccccccg caacggcctc cacgacccgc tccagctcag cgagaccatc cgccagacgc
     5221 tgccgctgct ctcccacggc gcctacgggg tgcccttcgg ccaccagctg ctgtggcagg
     5281 acttcagctg ggagatcgac cgtgaggcgt cacgctccga cggctctccc gccgaactgg
     5341 agctgcgcat acggtgcttc gacgtcacct accgcaagga ccgggcgtcc gcgatgtccc
     5401 tgaacatcga ggccgaacgg tcgggccgtc atctggccac cgcccgcacc cggttcacca
     5461 tccaggaccc gacggtctac cgccggctgc gcggacggta cgccgacaag gccgccgcca
     5521 acgcgggcgc cgtcccgctt ccgccgccgg ccgcgatgag ccccttcggg cgcgactcgt
     5581 tcgcggacgt cgtgctcgcc tccaccgacc tgccccaccg ctggcagctg cgggccgaca
     5641 ccgcccaccc catactcttc gaccaccccg tcgaccacgc tcccggcatg ctgctgctgg
     5701 aggcggcccg gcaggcggcg ctggccaccg gccaccccga acagcgggtc gtgctcggca
     5761 tggcctccgt gttcagccgc tacgccgaac tcgactcccc ctgctggatc accacggagc
     5821 cgatcgcggc cggggatgaa cagcgtcgcg gggagtggag cgcacagggt gaccgcaccg
     5881 cgaaccgggt gcgtgtctcc gcggagcaga acgaccggga gatcttctcc tccgaggtga
     5941 cgctcgctcc gctgcacccg gcgccgctcg cctgacggac cctgcggggg cgctcgtgcc
     6001 ggccggcacg agcgctggcc caccggcggc gggtacggac gccggccggt tcctgcgccg
     6061 gtgccacgac cgtcgtgcgg tcaggccgag ttgccgcgtc cggctgccgc acaacaagag
     6121 agagcccctg ccggacgaat ccggcagagg ctctcagctg cggtggacct gtggggattt
     6181 gaaccccaga ccccctcgat gcgaactatc ggcggggcgg tcgctgaggt tcgctgtggt
     6241 ggtcgagcgg gttactgaga ccgctggctg gtcggccgtg gtcgccgtgg ttgctgtact
     6301 tcactgctgt acagcaacca tcgctagggc cagagcaact tatcgaggat gatcgccgcc
     6361 actgcagaga agaaccacgg cttcctgacg ccccggacaa cagcatcacg gtccagttcc
     6421 ctgatgagca ccaccaaggc gaacatcagg agtgcgcccg gtagcaggcc aacgacgggt
     6481 tcggtatgcc gagatggtaa ccaggtgctg cggcgtcacg tcctactcgt cgcatgctaa
     6541 gtgcaggcag ccaggccaca tccaccgcag gtgaactgcc ccctgacagg caactcggca
     6601 ggcgccgttc tcgtgaggct tggccgccag cgaccgctgt tccctgctgt tcccgaccgg
     6661 acgggcgcgg gaagggcgcg gtctccttcc gatccgtagc tctagcgaga acggtcagca
     6721 acggtggccc gtcagcctcc cgggaccggc aaccgtgagc gggcgaatgt caggcggccg
     6781 ctgcggccag cttcgggttc gggccgtgct cgttctcctc cggcttgccg tcgctggcca
     6841 ggaagaccag caggacgata gcgcccacca gcgggaccag ggcgatgagc agccaccagc
     6901 cggagcgtcc ggtgtcgtgg aggcggcgga cgccgacggc gaggctgggg agcaggacgg
     6961 cgagctcgta gatcccggcg atgacgccgg tgccgaggag gctgaggtcg atgaccatca
     7021 gcgcgatata gatgatcgcg ctgatgagcg tgaacatcca gtattcctgg cggcgggcgc
     7081 ggccgctgaa tgcggcgtac ttcttgagga cgtcgatgta ccagtgcatg ggtcccccca
     7141 gggacgagcg gtggaggccg gtccgaaaag aacaagccag cgcggaatgt agatggcttg
     7201 atgcacaccg gtcaaaaagt tatcgggcag cgaccgatcg gtgatcacgg ttggctcgat
     7261 aagccctgct ttcacgccag atgcgactca aggggctgac cacgttgtgc cattccatgt
     7321 cgccaacata tgagcagctt gggctttgcg gcagcctctg cgtagacgtc aacagaggtc
     7381 ggtgtagcgg ctttgggcgg gagctgccat ggggcgagag atcggggccc gtaagcttcc
     7441 cgcgactcgg gcagtctctg gacctgcccg caatagcccg atgtgggccc cgatcttcga
     7501 ggctcgcccc atggtggctg cctaaagccg tggagccgac cgccccagcc ctgccggcct
     7561 ttctctgacg gcttggtgtc cgcgctcggt gcgccgccgc ccggccgggg cagcccggcg
     7621 aagacagggg ctgccccggg ccgggggcag cggcgcgccg cgcttgcgcg gcgccttgaa
     7681 cccgtagaga aggtttctac tcactccgcc cagtggcatc ggggatgagc cgactggtga
     7741 acccctgggc ctgcaagcgc tcgtaggcat cctggacgtc ctgtggaacg tcctggctga
     7801 tcatgaagcc ctgggccgga tagatcagta ggcgctgaag cgctccgacg agcatgtcaa
     7861 cgacgagtcg ggcatcaccg atcgagcgga cgtagtcggc caggggcatg ggtgctgacg
     7921 cgctgacgaa ctcgacttct ggccacagct tgcgcgccgt ggcgtaggcc cgacgttcct
     7981 cgtacggctt gctcaccagc agtacggacg acacggggac gccttgctcc tccagtagtg
     8041 cgcgtgagaa gcggatgttc tccccggtgt tccgggcccg tggctccacg aggatggcgt
     8101 cgtcgggaac gccaagctcg atggcacgct cccggtagtg ctcggcttcc ccgcggggca
     8161 tccgctcgcg agtcgttcga ctggtggctc ccgtgaagac gacaacaggc gccatgccct
     8221 ggcggtacag atcggcagtc acgtcggcta cgcctaggtc gtgacttccc aggccgatga
     8281 cgacggagca cggccgcggg gcgtgtccca tgagctggta gtcccacagg tgttgggcat
     8341 ccttcatgac cgcgtccgac agggtcatga gccctccttc ggctgcgccg agtccggcac
     8401 cttgaagtcg tactcccacg cgaaccgcga ggctgcgtgg atgcctacgg cgaactcgac
     8461 cacggtcccg tccgccgtgt aagtagtccg gtggagctcc atgaccggct cacctggtgg
     8521 cagttgcagg agcttggcct cctcggccgt gggcacgcgc gcgaacagtg cttctcgcat
     8581 gtggtcgatc tcgtacccgg cgtcatagag caccctgtag ccaccgcccc tgccggccgg
     8641 tccgggcgtc gggtccacca gacgcgtccc ctcgacatgc tccggccggt agtagctggt
     8701 cagggagtgg gtcggctgct caccctcctt caccaggcgg gcacgggcat agacctcgga
     8761 atctgctggg acaccgagag cttcggcaac cttgggcggt gccgggacgc gactgactgt
     8821 ctgggtctgc tcgttgcggc ggaaggcacg ccccgaagcc acgcggtcgg caatgaacgc
     8881 gacctcgtca ccatcgcgcc acttggcctt gtcgtagcgt ccgatgccga gacgcttcag
     8941 cggcgcctgc tcgcgcacca ccgtgccgtg tccgcgtgaa gacgtgacga gaccttcagc
     9001 ctccagagcc ttataggcgg catgcaccgt cgacttggag ccctcaccct tctccaccag
     9061 ttcgcggata tggggaagct gctggccagg cggacgctcg ccgctcctga tctcctcagc
     9121 cagccggtcc gccagctctc gccacttcgg tgccacgggg cctccttcgc ctacaccgcc
     9181 agtgaagcac agtcctagga cattggcagt cctgggactg cgctgcattc ttaggtcagg
     9241 cgctcgcagt cctaggacag tgaaggggtc gtgtggcctc gtgcgcgttg actcaaggag
     9301 tggctatgtc gtccttcaag atcgacactt cgaccgctgt cgtgttcgtc gccgtggagc
     9361 cgaaggcgaa ggtcatcaac aagcagaccg gtgagatcgc ggtcgaccgg gagaccggcg
     9421 cgaagatgat gacggtcggc ctgaccatcg cggacgaggg tgaggcgaac ctctacaccg
     9481 tctcgatccc ggagaccggt gtcccggagg ggatcacgct ggggatgccg gtcaaggtcg
     9541 tcggcctcaa ggcgcgagac tgggagaaca ccttcaacgg tgagaagcgg ttcggcatcg
     9601 ccttccgcgc ggtcgcgctc gttgtcccgt ccgtcaaggg ctgagcggca tgtcgaccac
     9661 gacgctcctc gtcttaagtc tggtggtggt cgccgcggct ctggtgctgc ggcggctgcg
     9721 cccggcctgg tactggctga gcatcggcgt cacgctcgcc acgctccgcc tggtggtccg
     9781 atacgcctcg gtcatggacg cgtgcgggct gaccgttccg ccgtcccggc tgcggctcac
     9841 cctcgcccgg ctcaccaatc ggccggcacc cgcgtcccgg ccgccgcgca tcctcagctt
     9901 ccgcccgacc cgcaccggcc tggtactgcg ggtcaagctt cggcccggtc aggacgcgtt
     9961 cgacttcgcg gcctccacgg accggctgcg gcactcgttc gccatgcacg gcatcacgtc
    10021 ccgtgaggtc aggtcgggtg tggtcgagct gcgcatgacc ggctacgacg tactggcacg
    10081 ggtgcacatg cccgccaagg tcgagcggga aggactgcgc atcccggtcg cgctgcggga
    10141 ggacggagca gtccactacc gcgattatcg ggaggtgccg cacgcgctga acctcggcgc
    10201 gacgaagtcg ggcaagtctg tctaccaacg ccacctggtc aaggaactcg cctcccggca
    10261 tgtcgctctg gtcggcatcg actgcaagca aggggtggaa ctcgccccgc tggctcgccg
    10321 gttctccgcg ctggccgaca acccggacga tgcggccgac ctgctggaag ctctcgtgca
    10381 gcgcatgggc gacacgtatc aggtgatccg gcgcgagcag cgcatcagcg cggaaacccc
    10441 ggataaggag atcaccgccg acctgtgggg cctgccggag aacctgcggc cggtgccggt
    10501 cgtgctcctg gtggacgagg tggccgaact ggcgctgttc tccaacacag cggagaagaa
    10561 gcgccgcgag cggatcatca ccgcacttgt gcgcctcgtg cagctcggcc gcgctgccgg
    10621 tatctacgtg gagatctgcg gtcagcgctt cggcgccgaa ctcggggacg gcatcaccat
    10681 gctccgcgcc cagctcaccg gccgcctctc gcaccgggtc aacgatgaag cctcggccaa
    10741 gatggccttc ggcgacatct cccctgacgc cgtgctggcc acgacacaga tcccggtgga
    10801 gcgtcccggc ctggcggtgg ccggtgactc ctcgggtggc tgggtccgca tccgtccgcc
    10861 gttcacgacg atgcgccaag cggtgaacgc ctgcaccgcc aacgctcacc gcaccccggt
    10921 cctcgagggc atggaggctt tccggccggt cctccctgac ctcgcgccgg tggag
//
