LOCUS       NZ_CP107567            10963 bp    DNA     linear   CON 18-NOV-2023
DEFINITION  Streptomyces peucetius strain NA0869 chromosome, complete genome.
ACCESSION   NZ_CP107567
VERSION     NZ_CP107567.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces peucetius
  ORGANISM  Streptomyces peucetius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8050611)
  AUTHORS   Zhao,E. and Ge,H.
  TITLE     Cytochrome P450 Catalyzes Benzene Ring Formation in the Biosynthesis
            of Trialkyl-Substituted Aromatic Polyketides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8050611)
  AUTHORS   Zhao,E. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) School of Life Sciences, Nanjing University,
            No. 163 of Xianlin Avenue, Qixia District, Nanjing, Jiangsu 210033,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: unicycler(0.4.8) v. v. 3
            Assembly Name                     :: NJU_NA0869
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/18/2023 06:49:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,216
            CDSs (total)                      :: 7,126
            Genes (coding)                    :: 6,825
            CDSs (with protein)               :: 6,825
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 144 of 303
            Pseudo Genes (incomplete)         :: 227 of 303
            Pseudo Genes (internal stop)      :: 22 of 303
            Pseudo Genes (multiple problems)  :: 85 of 303
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:23:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3257947
            Orig. end                         :: 3268910
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107567.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from IFB-NA0869, Nanjing
            University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10963
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="9"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10963
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10963
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5963](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5963)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(75..1310)
                     /locus_tag="OGH68_RS14755"
                     /old_locus_tag="OGH68_14755"
     CDS             complement(75..1310)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037816.3"
                     /locus_tag="OGH68_RS14755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14755"
                     /product="RNase A-like domain-containing protein"
                     /protein_id="WP_264244291.1"
                     /transl_table=11
                     /translation="MSVEETAKDVLMKMGMWWPDANSGSLRAAAGHWRDFADAVDDVCR
                     ISNGKAASLIRNNKGEAIDAFEVFWSRYHRECAQGWLDDLAAVARQMAKALEEFADAVD
                     DAIQQLWTEIGIAAATIAAGIGLAVFTFGLSTAASAAAATTIIELAAGLGVTVSTTVAN
                     IAAITLTGVVFGSIESVTVELAVAQPLKISTGLQDGFNLSQAQDAAAYGAVFGGALGAG
                     AGTVRAASDAGGFVKMFQGIPINVRGPELAYPNGHLLLRESDYNAWPRSKRRGQLNPPQ
                     HRADIAGDEGKNGSHTIDRHVDVTTHYLRDRLRNNPGMGVASRYVDEASAQKFTDAAMM
                     GHQKEITAWLAGNKKKFDFSAHFDEFTGVSMTRHNFRNGLPVEWVNGVQVVLKRDPSAE
                     SGYRILTSYPIN"
     gene            complement(1307..1666)
                     /locus_tag="OGH68_RS14760"
                     /old_locus_tag="OGH68_14760"
     CDS             complement(1307..1666)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737953.1"
                     /locus_tag="OGH68_RS14760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14760"
                     /product="hypothetical protein"
                     /protein_id="WP_264244292.1"
                     /transl_table=11
                     /translation="MVETPKQILDIKTADLKASAPTFQEQSAALQLAAKTLAEKLGELG
                     TPWGDDEQGSQFADTYLPHVKQIKQSTGILIDGLASIHLAMTDMADGHIENEALIAAMF
                     ARVKADGGAHDGSAK"
     misc_feature    1622..1636
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    complement(1626..1644)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.6"
                     /tool="antismash"
     gene            complement(1828..2282)
                     /locus_tag="OGH68_RS14765"
                     /old_locus_tag="OGH68_14765"
                     /pseudo=""
     CDS             complement(1828..2282)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056397.1"
                     /locus_tag="OGH68_RS14765"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14765"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTESTANPATTSATPSATPSLAPRLLPWPSPEGKPCLLVADDHGS
                     RLSRLADEMEAVHLPRASTCSTTPATYSTNSCPPTAKCVTPESGSPSA"
     gene            2311..2574
                     /locus_tag="OGH68_RS14770"
                     /old_locus_tag="OGH68_14770"
     CDS             2311..2574
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002530.1"
                     /locus_tag="OGH68_RS14770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14770"
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_264244295.1"
                     /transl_table=11
                     /translation="MSDLPRYEYVKLADAIAAEIASGKLPLGAALPGERAMTELYSVSI
                     GTVRRAITELRKRELVAALPAKGTYVIATPAPDSSNSETDAD"
     gene            complement(2706..4085)
                     /gene="pglX"
                     /locus_tag="OGH68_RS14775"
                     /old_locus_tag="OGH68_14775"
     CDS             complement(2706..4085)
                     /EC_number="2.1.1.72"
                     /codon_start=1
                     /gene="pglX"
                     /inference="COORDINATES: protein motif:HMM:NF033451.1"
                     /locus_tag="OGH68_RS14775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14775"
                     /product="BREX-2 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="WP_319020208.1"
                     /transl_table=11
                     /translation="MLNSSAACFWLKQVSQGKGGSGLGRGVQDEVWEERYEFTGTKLQD
                     FPLSVTMPLAVACERDSLAQRLFELEPSAMAGRAIATRHGLAGASDAQGRTRSRMIALQ
                     EELDWTVYGAYGLLTPKEVAQTTLPGDPADLSDADVPQLQLGQRAFEIALKRKVDAGDA
                     ETAWFERHGSTDTAVAELPADWPEAYQKVVQARLDIIESNADIRLLERPEYKRRWSIEL
                     WEKREAAALRAWLLEAAEREDLWFEERDEYTSPRTLTVFQLADALRHDENVQAVAALYA
                     ANHLDNREASLATVLKHVIESEHVPYLAALRYKESGLRKRDEWESVWEQQREEDRTGER
                     LDIKVPPKYSGADFLKHSYWSNRGKLDVPKERFISYPGASPDSDPSLLLGWAAWNHRDQ
                     GEALVNLINDRASIDGWELEDPRFVPLLASLREVMPWVRQWYAPTTRSGVTIPRGSSRP
                     R"
     misc_feature    4094..4105
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    4106..4120
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.52"
                     /tool="antismash"
     gene            complement(4109..4870)
                     /locus_tag="OGH68_RS14780"
                     /old_locus_tag="OGH68_14780"
     CDS             complement(4109..4870)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006124137.1"
                     /locus_tag="OGH68_RS14780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14780"
                     /product="transposase family protein"
                     /protein_id="WP_264242147.1"
                     /transl_table=11
                     /translation="MVPHRAMLDVPHEVVEHVSWLIYARRCELNSRWRRLGCFRQALLV
                     LVHLRKNETLAQVAAGFGVSTATAGRYVSETVDILAERAPSLHEALRELPPEGFVILDG
                     TLIATDRIAADEPYYSMKHRRHGMNVQVVAAPDGTPLWFSRALPGRTHDLTAARAHGVI
                     QACLSRQLLVLADRAYRGAGATVRTPYYGRDLPEKYAQFNRDHARLRAPGERAFARLKQ
                     WRILRKARCSTNRIGRTVAAVHAIEIAWSSG"
     gene            complement(5001..5963)
                     /locus_tag="OGH68_RS14785"
                     /old_locus_tag="OGH68_14785"
     CDS             complement(5001..5963)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="OGH68_RS14785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14785"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_264244299.1"
                     /sec_met_domain="AfsA (E-value: 6.5e-20, bitscore: 69.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDPPRALLAPQYAMDTADLRFVRTAPRHLVGRSAVAEVLITDWR
                     QLGPDTFRLGAQWPRGHTFYPPIAGAWHDPLLAAETLRQASVLVGQTYYGVPAGRRPLM
                     DALDIEVVPGALLLGPHPAEIRLEVSGADVGRTHGTLTGMTLEVELMRGDEFVGAGRLA
                     LRVPSADGREHRDRTRSGDGLPPRPEPVPAAIVGRLDERDVVLGVPSSAAARRGHNWTL
                     RIDPGHPVLFDRPADHVPGMMLLEAARQATQAVCAPYRVLPLELRSAFHGFVELDIPCH
                     IAATRLPSQDAGEDAVVRVTGSQDGKLLFDSLVVAGLCD"
     gene            complement(6202..6678)
                     /locus_tag="OGH68_RS14790"
                     /old_locus_tag="OGH68_14790"
     CDS             complement(6202..6678)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1160: flavin reductase domain protein FMN-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378329.1"
                     /locus_tag="OGH68_RS14790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14790"
                     /product="flavin reductase family protein"
                     /protein_id="WP_264244300.1"
                     /transl_table=11
                     /translation="MLEQRSFRDILGRFTTGVVLITAQTEQGPVGMAVNSFTSVSLDPP
                     LVALCAAETSTTWPAIRETGSFAVTILGDRHGDLCRLFSTKGAERFTGREWSTTRDGHP
                     IPADGLGWLDCRITTIHQAGDHELVVAEATEGAVTEQIGPLVFHAGRLTELATV"
     gene            complement(6769..7878)
                     /locus_tag="OGH68_RS14795"
                     /old_locus_tag="OGH68_14795"
     CDS             complement(6769..7878)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1251: luciferase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378325.1"
                     /locus_tag="OGH68_RS14795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14795"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_264244301.1"
                     /transl_table=11
                     /translation="MHSRIGLFLSPVHETGQDPHLAIRRNLDLVEQADELNFDEAWFGE
                     HHTLGWGLVGAPETLIAAASQRTRQIKLAHGVVPLSGHHPFHVASRAVHLDHLTRGRYI
                     LGVGPGVPFDAKMFGLEQPVQRRRLEEALPTVLELVNGDERITQQTDWFDLRDAKLQLP
                     RFSPDGIEVAMATSGTSQSSPRLIGQYGLSMTSFALPFALMTPGAPAHINLAHQWKFAE
                     ESAAEHGRTLDRDKWRIALPVHVAETREEAYADVREGYDRWLFEYFGKAAGRDVISPDV
                     PREKALEARVEAGGALVGSVDDVVEGIRRLHEITGGFGTLLVYVADWTSWEKTNRSMEL
                     LARYVTPRITGSADRPQEAVDWAIASRAK"
     gene            complement(8120..9376)
                     /locus_tag="OGH68_RS14800"
                     /old_locus_tag="OGH68_14800"
     CDS             complement(8120..9376)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412411.1"
                     /locus_tag="OGH68_RS14800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14800"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_264244304.1"
                     /sec_met_domain="RimK (E-value: 3.8e-05, bitscore: 22.1,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 8.8e-10, bitscore:
                     37.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSARKPLAVVLDGYSAGNFYPAAFAAQGADVIHVQSTAELIPTM
                     QAPDLSAYGENIVCTDEDKLVEILREHDPLCVVAGQESSVRLADRLSERLGLPTNGSAL
                     SPARRDKYEMIEALRRAGVRCAAQFKSGDPDALTAWAKEQGRYPVVIKPISSAASDGVF
                     ICESAGEVRTAAETVLASQDIFGTSNSEILVQSYLKGAEYIVDTVSCDGERYACGVWQY
                     EKTLLPSGKNIYNRDILVDPDSELVTALVSYVDEVLAALGVRWGPAHAEVIMTDEGPAL
                     VEIGTRLNGNINAPFHDVCLGHNQADLAAQAYAQPESFRALYGGRVYRKLQPAVVYNTP
                     TELDGIVASVDETVVAEIRGLESVFFAGVKVRPGSPIRPTVDLLTSSLRVFLTAADEER
                     LAADYDRVRTLKDAVYQLA"
     gene            complement(9373..10710)
                     /locus_tag="OGH68_RS14805"
                     /old_locus_tag="OGH68_14805"
     CDS             complement(9373..10710)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (88-435). E-value:
                     4.2e-23. Score: 74.0. Matches aSDomain:
                     nrpspksdomains_OGH68_RS14805_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378342.1"
                     /locus_tag="OGH68_RS14805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OGH68_14805"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_264244306.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.5e-21, bitscore:
                     74.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVTPVQEQPPLQRTPLLRTSLHAGIVDPEMDSMRLLSETAMKFP
                     EALSFSSGAPYDGNHDLANLSHYVERYLRHLRDQGVPEQRITRLLFQYGPVNGFIQDEV
                     ARMLRHDEGIEVSAESIMITHGFQEATLVALRGLFASPDDVLLSVSPAYVGIRGAARML
                     DIPVKGIVEGPNGLEPEAVAAAARAVRATGKRPRALYLVPDFSNPSGTVVPLEARQRLL
                     EVAADEGLTILEDNPYGLFARDGEKMPTLKSLDTRGDVIYLGSFAKSAFPGARLGYLVA
                     DQEVTGEDGSRRTLAQELSKAKAMFSVGSSSLSQAAIGGILVENDYDLTSATRELAAVY
                     QERLAASLDALAEHFPPERYAEHQVRWNVPRGGFFLTVEVGFEADLAAMERSARDFGVS
                     WAPMSMFYLDGGGDRALRLGFSNLTPAAIREGIARLARFIESEAGA"
     aSDomain        complement(9406..10446)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_OGH68_RS14805_Aminotran_1_2.1"
                     /evalue="4.20E-23"
                     /label="OGH68_RS14805_Aminotran_1_2.1"
                     /locus_tag="OGH68_RS14805"
                     /protein_end="435"
                     /protein_start="88"
                     /score="74.0"
                     /tool="antismash"
                     /translation="LLFQYGPVNGFIQDEVARMLRHDEGIEVSAESIMITHGFQEATLV
                     ALRGLFASPDDVLLSVSPAYVGIRGAARMLDIPVKGIVEGPNGLEPEAVAAAARAVRAT
                     GKRPRALYLVPDFSNPSGTVVPLEARQRLLEVAADEGLTILEDNPYGLFARDGEKMPTL
                     KSLDTRGDVIYLGSFAKSAFPGARLGYLVADQEVTGEDGSRRTLAQELSKAKAMFSVGS
                     SSLSQAAIGGILVENDYDLTSATRELAAVYQERLAASLDALAEHFPPERYAEHQVRWNV
                     PRGGFFLTVEVGFEADLAAMERSARDFGVSWAPMSMFYLDGGGDRALRLGFSNLTPAAI
                     REGIARL"
ORIGIN
        1 ggaacgcagc aggttccgca gctccttgaa gcggtcgctg tacgacgtca ttgatgtctc
       61 aactggtttc tgtgttagtt aatgggatat gaggtgagga tccgataccc tgattcggcc
      121 gacgggtcgc gcttcaggac cacttgtacg ccgttgaccc attctaccgg caatccgttc
      181 cggaagttgt gccgggtcat gctcacgccc gtgaactcgt cgaagtgcgc cgaaaaatca
      241 aacttcttct tgtttccggc gagccaggcg gtgatctcct tttggtgacc catcatggcg
      301 gcgtccgtga acttttgcgc ggatgcttcg tccacatatc gggacgccac gcccatgccg
      361 ggattatttc gcagccggtc ccgcaggtag tgcgtcgtga cgtccacatg acggtctatg
      421 gtgtgcgaac cgttcttccc ctcgtccccc gcgatgtccg ccctgtgctg cggtggattg
      481 agctgccctc ttcgcttcga gcgcggccat gcgttgtaat cactctctcg tagcagcagg
      541 tgcccgttgg ggtaggccag ttcggggccc cgcacgttga tcgggattcc ctggaacatc
      601 ttgacgaacc cgccggcgtc cgatgcggct cgtacggttc cggctcccgc gccgagcgcg
      661 ccgccgaaga cggctccgta ggcggcggcg tcctgcgcct gggagagatt gaagccgtcc
      721 tgcaggccgg ttgagatctt caagggctga gcgaccgcta gctcgaccgt cacggactcg
      781 atgctgccga agacgactcc tgtgagcgtg atcgctgcga tattggccac agtcgtcgat
      841 acggtgactc cgagccctgc tgcgagttcg atgatggtgg ttgcggcggc agccgaagcg
      901 gcagtggaga gaccgaaggt gaagaccgcc agcccgatgc ccgctgcgat ggtggctgcc
      961 gcgatcccga tctctgtcca aagctgctgg atcgcgtcgt cgactgcgtc tgcgaactcc
     1021 tccagcgcct ttgccatctg acgtgccact gccgccaggt cgtccagcca gccctgggcg
     1081 cactccctgt ggtaccggct ccagaagacc tcgaaggcgt cgatcgcctc gcccttgttg
     1141 ttgcggatga gggatgcagc cttgccgttg gatattcggc acacgtcatc gacggcgtca
     1201 gcgaagtcac gccagtgccc tgccgccgcg cgcagactgc ccgagtttgc gtccggccac
     1261 cacatcccca tcttcatgag gacgtccttg gcggtctcct cgacgctcac ttggcgctgc
     1321 cgtcgtgcgc cccgccgtca gccttgaccc ttgcgaacat ggcggcgatc agcgcctcgt
     1381 tctcgatgtg cccgtctgcc atgtccgtca ttgccaggtg aatgctggcc agaccgtcga
     1441 tcaggatgcc ggtcgactgt ttgatctgtt tcacgtgtgg caggtaggtg tctgcgaact
     1501 gcgatccctg ctcgtcatcg ccccagggtg tgccgagctc accgagcttt tccgccagag
     1561 tcttggcggc aagctgcagc gccgcgctct gttcctggaa tgttggagcc gacgccttca
     1621 gatcagctgt cttgatgtcg agtatctgct tcggtgtctc aaccaccgca agtctccccc
     1681 gtgttgtagt gcaggcctga tgacggtagc tcatcgacat gtgcacacca caaggaggtg
     1741 atcggtcaaa ggatgccgtt gaggggttcc acgtcgtcgt gtggcctcgg tacttgatca
     1801 cgcaactacg ccgaagccct gcacgtgtta gcggacggcg acggcgcgac gtgaggcagg
     1861 cgttctccag tcggcgtgtg aagtgccggc atcgggatca cctggcacag tgccggtctt
     1921 gagagtgtcg gttgcgtcgt cgaagtcgtc ggggccggaa aggcgcaagc cgcgggactt
     1981 cgccacgcgc agggcgtcgc tcaggcactc ggcgagcctg attccggcgt aacgcacttc
     2041 gccgtaggtg gacatgaatt cgtcgagtac gtcgcgggcg tggtcgagca cgtcgaggcc
     2101 cgtggcaagt ggacggcctc catctcgtcg gccagccgtg acaagcggct gccgtggtcg
     2161 tcggcgacga gaaggcaggg cttgccttcc ggcgagggcc acggcagcag ccggggtgcc
     2221 agggaggggg tggcggaggg cgtcgcggaa gtggttgctg ggtttgccgt cgactcggtc
     2281 atgccgctgc cccctgcgcg ccgactacag atgagcgatc tcccgcgcta cgagtacgta
     2341 aagcttgccg acgccatcgc tgccgagatc gcatccggca agctgccgct aggtgcagcc
     2401 ctgccaggcg agcgagccat gacggagctt tacagcgtga gcatcggaac cgtgcgccgc
     2461 gcgatcacgg aactacggaa gcgggaactc gtcgccgccc tgcctgcgaa ggggacatac
     2521 gtcatcgcga caccggcgcc cgactcgtca aactcggaaa cggacgccga ctgacgcgtc
     2581 agcgaagatg gcattgcggg cagccctcac aatctattcg gccttcctcc ggccgccctt
     2641 ggccctcacc ggacgccagg cgatcaggtc tgcctcagag agcgagcggt cggcgcaccc
     2701 tcggttcagc gcggtctgga actcccgcgc gggattgtca ccccactcct cgtcgtaggc
     2761 gcgtaccact ggcgcaccca gggcatgacc tcgcgcaggc tggcgagcag cggtacgaag
     2821 cgggggtctt ccagctccca cccgtcgatc gaagcacggt cgttgatgag gttcaccaac
     2881 gcctcaccct gatcacggtg gttccaggcg gcccagccga gcaggaggga cggatcactg
     2941 tccggggagg cccccgggta ggagatgaac cgttccttgg gcacgtccag cttgccgcgg
     3001 ttcgaccagt acgagtgttt gaggaagtcc gcaccgctgt acttcggggg gaccttgatg
     3061 tcgagccgtt cgccggtacg gtcctcctcg cgctgctgct cccacaccga ctcccactcg
     3121 tcgcgcttac gcaggccgga ctccttgtag cggagagccg cgaggtacgg gacgtgctcg
     3181 ctctcgatga cgtgcttgag caccgtcgcg agcgaggcct cacggttgtc gaggtggttg
     3241 gcggcgtaca gcgcggcgac cgcctggacg ttctcgtcgt ggcggagagc atcggcgagc
     3301 tggaacacgg tgagtgtgcg gggcgaggtg tactcgtcgc gctcctcgaa ccacaggtcc
     3361 tcacgctcgg cggcttcgag cagccatgcc cgcagggccg ctgcctcgcg cttctcccaa
     3421 agctcgatgg accagcggcg cttgtactcg gggcgctcca gcagacggat gtccgcgttg
     3481 gactcgatga tgtcgagcct cgcctgcaca accttctggt acgcctcggg ccagtcggcg
     3541 gggagctcgg cgacggccgt gtcggtcgac ccgtggcgct cgaaccaggc ggtctcggca
     3601 tcacctgcgt cgacctttcg ctttagcgcg atttcgaaag cccgttgccc caactggagc
     3661 tggggcacgt cggcatcgct cagatcggcg gggtcaccag ggagcgtggt ctgcgcaacc
     3721 tccttgggtg tcaggagccc gtacgcgcca tacacggtcc agtccagctc ctcctggagc
     3781 gcgatcattc gcgaacgggt tcggccctgt gcgtcggagg cgcctgcaag gccatgacgt
     3841 gttgcgatcg ctctgccagc catagcggag ggttcgagtt caaacaatct ctgtgcaagc
     3901 gaatcccgtt cacaggccac agcaagcggc atggttaccg aaagggggaa atcctgaagc
     3961 ttcgtgccag tgaactcata gcgctcttcc catacctcgt cctgtacacc gcgccccagg
     4021 ccacttcccc cctttccctg actcacttgc ttcagccaaa aacaagccgc tgaagaattg
     4081 agcaccccga tcagtcagtg aacgcttctc atcctgagct ccaggcgatc tcgatggcgt
     4141 gaacggcggc gaccgtgcgg ccgatgcggt tggtgctgca gcgggctttg cggaggatcc
     4201 gccattgctt gaggcgcgca aaggcacgtt cgcccggcgc ccgcagccgg gcgtggtccc
     4261 ggttgaactg ggcgtacttc tcgggaaggt ctcggccgta gtagggggtg cggacggtgg
     4321 ctccggctcc gcggtaggcc cggtcggcaa ggacgaggag ctgacgggac agacaggctt
     4381 ggatcacacc gtgtgcgcgg gctgcggtca ggtcgtgcgt gcggccgggc aacgctcgcg
     4441 agaaccacag cggtgtcccg tccggcgcgg ccacgacctg cacgttcatc ccgtgacggc
     4501 ggtgcttcat cgagtagtac ggctcgtccg ccgcgatgcg gtcggtggcg atcagggtgc
     4561 cgtcgaggat gacgaacccc tccggcggca actcgcgcag ggcctcgtgc aggctcgggg
     4621 cgcgctcggc gaggatgtcg accgtttcgc tcacgtagcg gccggcggtc gcggtggaga
     4681 cgccgaaccc cgctgcgacc tgggccagag tctcgttctt gcgcaggtgc accaggacaa
     4741 gcagggcctg ccggaagcag cccagcctgc gccagcggga gttcagctca caccttcggg
     4801 cgtagatgag ccaggaaacg tgctccacga cctcgtgcgg gacgtcgagc atggcacgat
     4861 gcgggaccaa cagggctcct gtgtcgagac gttggcgagt gagatcacca gccgaaacga
     4921 ccaggagccc tgccgcgtta cggcccccga ccgccagccg tcatcacccg cgaaccacag
     4981 gatgagaagc gttcagtcac tcagtcacag aggcccgcca ccaccaggct gtcgaagagc
     5041 agcttgccgt cctgcgaacc ggtcacccgc accacggcgt cctcgccggc gtcctgggaa
     5101 gggagcctgg tcgctgcgat gtggcacggg atgtcgagtt ccacgaatcc gtggaaggcg
     5161 ctgcgcagtt cgagcggcag gacgcggtac ggggcgcaca cggcctgggt cgcctgccgg
     5221 gccgcctcca gcagcatcat gcccgggacg tggtcggcgg ggcgatcaaa gagcaccgga
     5281 tggccggggt cgatccgcag ggtccagttg tggccacggc gcgcggcggc cgaggacggg
     5341 acgcccagga cgacgtcacg ctcgtcgagc cggcccacga tcgcggccgg gaccggctcc
     5401 ggccggggcg gcagcccgtc cccggaacgg gtccggtccc ggtgctcccg gccgtcggcg
     5461 gagggcaccc gcaacgccag ccggccggcg ccgacgaact cgtcgccgcg catcagctcc
     5521 acctccagcg tcatgccggt cagcgtgccg tgcgtgcgtc cgacgtccgc gccggagacc
     5581 tccagccgta tctcggccgg atgcgggccc agcagcagag ctcccggcac gacctcgatg
     5641 tccagcgcgt ccatgagggg ccgccggccc gccggtacgc cgtagtaggt ctggccgacc
     5701 agcacggacg cctgccgcag tgtctctgcc gcgagcagcg ggtcgtgcca tgcgccggct
     5761 atcggcgggt agaaggtgtg cccgcgtggc cactgggcac cgagacggaa ggtgtcggga
     5821 cccagctgcc gccagtcggt gatcagcact tcggcgacgg cgctgcggcc gacgaggtgg
     5881 cggggggccg tcctgacaaa gcggagatca gccgtgtcca tggcgtactg cggtgccagg
     5941 agggcgcgcg ggggatcggt cacggacacg caggattgcc tttctcaatg gtcggcggtg
     6001 cggagtgcgt ttcccatggg gcggactgcg agagccggac gcgtcgcggc acgaacgggc
     6061 gggcggtgca cggccctggg gcttcggcga ccggtgagga ggacgggccg cggaggggac
     6121 ggtcggccgg ccggggaaga gggggcgggc cgcgtaggag gtggcggggc ggcccgcccg
     6181 tcgagccccc gccggccggc atcagaccgt cgccagctcc gtgagacggc cggcgtggaa
     6241 gaccagtggc ccgatctgct cggtgaccgc gccctccgtc gcctccgcga cgacgagttc
     6301 gtggtccccg gcctggtgga tcgtcgtgat acggcagtcc agccagccca gcccgtccgc
     6361 cgggatgggg tgcccgtccc gcgtggtgga ccattcccgg ccggtgaacc gctcggcccc
     6421 cttggtgctg aacagccggc acaggtcccc gtgccggtcg ccgaggatgg tgaccgcgaa
     6481 ggagcccgtc tcacggatgg ccggccatgt cgtcgacgtc tccgccgcgc acagggcgac
     6541 cagcggcggg tccagggaca ccgaggtgaa ggagttgacg gccatgccca cggggccctg
     6601 ctcggtctgc gcggtgatca gcacgactcc cgtggtgaag cggccgagta tgtctctgaa
     6661 cgagcgctgc tccagcatct gttcttccgc tcaagtgaga agtgggtgaa ggtcggttcg
     6721 ccgcgggtac cggccggacc ggcgggaccg gccggcaccc gcaccggact acttggcgcg
     6781 actggcgatc gcccagtcga cggcctcctg cgggcggtcg gcggagccgg tgatacgggg
     6841 cgtcacgtac cgcgcgagca gctccatgct ccggttcgtc ttctcccagg acgtccagtc
     6901 cgccacgtac accaggagcg tgccgaaccc gccggtgatc tcgtggagcc gccggatgcc
     6961 ctccaccacg tcgtcgaccg acccgacgag ggcgccgccg gcctcgacgc gggcttccag
     7021 tgccttctcg cggggcacgt cgggactgat gacgtcccgg ccggccgcct tgccgaagta
     7081 ctcgaacagc cagcggtcgt agccctcacg cacatcggcg taggcctctt cgcgggtttc
     7141 tgccacatgc accggcaggg cgatgcgcca cttgtcccgg tcgagggtcc ggccgtgctc
     7201 ggcggcggac tcctcggcga acttccactg gtgcgccagg ttgatgtggg cgggcgcgcc
     7261 cggcgtcatc agcgcgaacg gcagcgcgaa cgaggtcatg ctcaggccgt actggccgat
     7321 cagccgcgga ctggactgcg aggtgcccga ggtggccatg gcgacctcga tgccgtccgg
     7381 gctgaagcgg ggcaactgga gcttggcgtc gcgcagatcg aaccagtccg tctgctgcgt
     7441 gatccgctcg tcgccgttga ccagttccag cacggtcggc agcgcctcct cgagccgccg
     7501 gcgctgcacg ggctgctcca ggccgaacat cttcgcgtcg aacggcaccc ccggaccgac
     7561 gcccaggatg tagcggccgc gggtcaggtg gtcgaggtgg acggcgcggc tcgcgacgtg
     7621 gaaggggtgg tgaccggaga gcggcacgac accgtgggcc agcttgatct gccgggtgcg
     7681 ctgggaggcc gcggcgatca gggtctcggg ggctccgacg agcccccagc cgagggtgtg
     7741 gtgctcgccg aaccacgcct cgtcgaagtt gagttcgtcg gcctgctcga cgaggtcgag
     7801 gttcctgcgt atcgcgaggt gcgggtcctg gccggtctcg tggacggggg acaggaagag
     7861 accgatgcgg ctgtgcacag caattctcct gggatgtgga agggcgggag ttcgaaggcg
     7921 tgcggaagca cggggaggcg tgcgaaccac ggagagacgt gcggaggcac ggaagacgtg
     7981 tggaggcacg gtgaagcgtg cgaaggcacg gaagacgtgc ggaggcacgg gaaggcggag
     8041 gccggggggt gtacggaggg ccgtcacggg cgccgcccgg ggctcgggcc gcggcgtgcg
     8101 gggcgcgccg gcggccgggt cacgcgagct ggtagaccgc gtccttcagg gtccggacac
     8161 ggtcgtagtc ggcggcgagc cgctcctcgt cggccgccgt gaggaacacc cgcagggaac
     8221 tcgtcagcag gtcgaccgtg ggacggatcg gcgagcccgg ccgtaccttc acgccggcga
     8281 agaagacgct ctccagcccg cggatctccg ccacgaccgt ctcgtcgacc gacgccacga
     8341 tcccgtcgag ctcggtgggc gtgttgtaga cgacggcggg ctggagcttg cggtagacgc
     8401 ggccgccgta cagcgcgcgg aagctctccg gctgcgcgta agcctgcgcc gcgaggtcgg
     8461 cctggttgtg gccgaggcac acgtcgtgga agggggcgtt gatgtttccg ttgaggcggg
     8521 tgccgatctc gacgagggcc gggccctcgt ccgtcatgat cacctcggcg tgcgccggac
     8581 cccaccgtac gccgagcgcc gccagcacct cgtcgacgta cgagacgagc gcggtcacga
     8641 gctcgctgtc cgggtcgacg aggatgtccc ggttgtagat gttcttgccg gagggcagca
     8701 gcgtcttctc gtactgccac actccgcagg cgtaccgctc gccgtcgcag ctcacggtgt
     8761 cgacgatgta ctcggcgccc ttgagatagg actgtacgag gatctcgctg ttggaggtcc
     8821 cgaagatgtc ctgggacgcg aggacggtct cggccgcggt gcggacctcc ccggcgctct
     8881 cgcagatgaa caccccgtcg gacgcggccg agctgatcgg cttgatgacc acgggatagc
     8941 ggccctgctc ctttgcccag gccgtcagcg cgtccgggtc gccgctcttg aactgcgcgg
     9001 cgcaccgtac gccggcccgg cgcagtgcct cgatcatctc gtacttgtcc cggcgggccg
     9061 gggagagcgc ggaaccgttg gtcggcagcc ccagccgctc gctcagccgg tcggcgagcc
     9121 ggacggagga ctcctggccc gcgacgacgc agagcgggtc gtgctcgcgc aggatctcga
     9181 cgagcttgtc ctcgtcggtg cagacgatgt tctcgccgta cgccgacagg tcgggtgcct
     9241 gcatcgtggg aatgagttcg gcggtgctct ggacgtggat cacgtcggcg ccctgtgcgg
     9301 cgaacgcggc ggggtagaag ttgcctgccg aatagccgtc gaggaccacc gcgaggggct
     9361 tgcgggcgga gttcatgcgc cggcctccga ctcgatgaag cgggcgaggc gcgcgatgcc
     9421 ctcgcggatg gcggcggggg tgaggttgct gaagccgagc cgcagcgcgc ggtcgccgcc
     9481 gccgtcgagg tagaacatgc tcatcggcgc ccagctgacg ccgaagtcgc gcgccgagcg
     9541 ctccatggcg gcgaggtccg cctcgaagcc gacctcgacg gtgaggaaga agccgccccg
     9601 gggcacgttc cagcgcacct ggtgctcggc gtaccgctcg ggcgggaagt gctcggcgag
     9661 cgcgtcgagg gatgccgcga gccgctcctg gtagacggcg gcaagctcgc gggtggcgga
     9721 ggtcaggtcg tagtcgttct ccacgaggat tccgccgatc gcggcctggg agagcgagga
     9781 ggagccgacc gagaacatgg ccttggcctt ggacagttcc tgggcgagcg tgcgccggga
     9841 gccgtcctcg ccggtcacct cctggtcggc gaccaggtag ccgagacggg cgccggggaa
     9901 ggccgacttg gcgaacgacc cgaggtagat cacatcaccg cgggtgtcca gcgacttgag
     9961 cgtcggcatc ttctcgccgt cccgggcgaa cagcccgtac gggttgtcct ccaggatcgt
    10021 caggccctcg tccgccgcca cttcgagcag ccgctgccgg gcttccaggg ggacgaccgt
    10081 gcccgacggg ttggagaagt ccgggaccag gtacagggcg cgcggacgct tgccggtggc
    10141 ccgtacggcg cgggcggcgg cggccacggc ctccggctcc aggccgttcg ggccctcgac
    10201 gatgcccttc accgggatgt ccagcatcct cgccgcgccg cggatgccca cgtacgccgg
    10261 cgagacggac agcaggacgt cgtcgggcga ggcgaacagg ccgcgcaggg cgacgagggt
    10321 cgcctcctgg aagccatggg tgatcatgat cgactcggcg gagacctcga tgccctcgtc
    10381 gtggcggagc atccgggcga cctcgtcctg gatgaagccg ttgacgggtc cgtactggaa
    10441 gagcagccgg gtgatgcgct gttcggggac gccctggtcg cgcaggtgcc tgaggtagcg
    10501 ctcgacgtag tgcgagaggt tcgccaggtc gtggttgccg tcgtagggag cgcccgagga
    10561 gaacgacagg gcctcgggga acttcatcgc ggtctcgctc agcaaccgca tcgagtccat
    10621 ctccgggtcg acgatgccgg cgtgcagcga ggtccggagc agtggtgtcc gctggagcgg
    10681 tggctgctcc tggacgggtg tgacggtcat gccggttgtt ccttccgacg tgtggggtcc
    10741 gggcgggggc ggaggcggag gcggaggcga acggtcccgg ggtgcggccc gggaagacgg
    10801 agcggtgcgc gtccggtcgg gcggggcggc gagcgtcccg tcaggcggcg ggccggtcca
    10861 gtgcggcgat ccagctgccc ttgcccggcg tccactgccg gacgcgggtc tcggcgacga
    10921 ggccgccccg gacatagggc tccgcgtcca gcagcttctg cag
//
