LOCUS       NZ_CP102865            20531 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Paenibacillus polymyxa strain S3 chromosome, complete genome.
ACCESSION   NZ_CP102865
VERSION     NZ_CP102865.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 5740272)
  AUTHORS   Yang,S., Jin,D., Li,H., Jiang,L., Cui,J., Huang,W., Rang,J., Li,Y.
            and Xia,L.
  TITLE     Screening of new Paenibacillus polymyxa S3 and its disease
            resistance of grass carp (Ctenopharyngodon idellus)
  JOURNAL   J Fish Dis 46 (1), 17-29 (2023)
   PUBMED   36097971
REFERENCE   2  (bases 1 to 5740272)
  AUTHORS   Yang,S., Jin,D., Li,H., Jiang,L., Cui,J., Huang,W., Rang,J. and
            Xia,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) State Key Laboratory of Developmental
            Biology of Freshwater Fish, College of Life Science, Hunan Normal
            University, Yuelu, Changsha, Hunan 410006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-JUL-2021
            Assembly Method                   :: SOAPdenovo v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164.83x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:01:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,183
            CDSs (total)                      :: 5,026
            Genes (coding)                    :: 4,956
            CDSs (with protein)               :: 4,956
            Genes (RNA)                       :: 157
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 111
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 48 of 70
            Pseudo Genes (internal stop)      :: 10 of 70
            Pseudo Genes (multiple problems)  :: 17 of 70
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:25:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4356381
            Orig. end                         :: 4376912
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Hunan Normal University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20531
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="14"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20531
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20531
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10531](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10531
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            116..1036
                     /locus_tag="NWS42_RS19720"
     CDS             116..1036
                     /GO_function="GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752601.1"
                     /locus_tag="NWS42_RS19720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidoglycan recognition family protein"
                     /protein_id="WP_263560041.1"
                     /transl_table=11
                     /translation="MFNPNKYTIERRYINKRHNVRPGTRLTSGNPVFLVAHDTGNPGAS
                     ADNHYTYFNGLVDRSASAQTFIDDKKILEIIPTGTGPDPAEKAWHVLYNVTTDNELYGD
                     DANDIALGVELCFGGKIDTLEAYKRFAWYLAYCCNKWGINPRTHIPSHKQLDPARKRDV
                     DQALATIGKTLKELVYDVETELKGASVAPAALDFTPLPVYIAQSLIDNYVSPAWFASQK
                     AADQEGKTHFHNLANNLRAAAGLNEKNLPLAGAVKLFKSNAQEIIFRWLQPAWFKAKQV
                     GDKEQMQHYNNLANYLRSAAGLPTQ"
     gene            1052..1603
                     /locus_tag="NWS42_RS19725"
     CDS             1052..1603
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014599907.1"
                     /locus_tag="NWS42_RS19725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phage holin, LLH family"
                     /protein_id="WP_263560042.1"
                     /transl_table=11
                     /translation="MQNVIETIQPAISTIVTAIVGVLVTFALAGINTLKNKANNWLDAR
                     TTAAQREVIHKVAGEAFALAQTTFKEAGGIRKMQEALQYASLRLTEQGIVVSPTELQAA
                     IEKAYLEYKAKTKAVLATEAQPNEEAAQVAAKEAVSGLAAKLNDFLAQATAEVSLGQVQ
                     TAPELIPAPTESEQAPVTTE"
     gene            complement(1951..2988)
                     /locus_tag="NWS42_RS19730"
     CDS             complement(1951..2988)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NWS42_RS19730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_263560043.1"
                     /transl_table=11
                     /translation="MEGLMRFIKENEIPIVMIPEDRRYWLVRTQSGEFYDEFFHDGFIG
                     IGWNEFSDIEKFLNTEKDEMTDLISITYKPDEGEKPPQPGRIYGQIYRFLSELNVNDIV
                     MIPSSNSSHISFGIIESEPYIEEVSETGLDMGLCPFIKRRDVNWIKTVQRDRLDPYLYR
                     MMQAHQAISNANDYANYIDRTLHSFYLKGDKAHLVVDVKQEKQIPAVDMISYVSTLLDI
                     VPLIENPEKPEETFKKEDVDLKLNVQSPGLMEFISQSPYVMMILGMVVVGLVGGSFKFK
                     NSNGKKESSNEVELQSEGLFEKVFKFIKHKDEHKLKEIKQQHSIYKDKLKAELPKELTE
                     KLDQS"
     misc_feature    2948..2962
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.5"
                     /tool="antismash"
     gene            3172..3639
                     /locus_tag="NWS42_RS19735"
     CDS             3172..3639
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686521.1"
                     /locus_tag="NWS42_RS19735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NucA/NucB deoxyribonuclease domain-containing
                     protein"
                     /protein_id="WP_263560044.1"
                     /transl_table=11
                     /translation="MRKTCITICLLGALIVFGGFINTLSHAEWGDKPRVQTSAPVSATT
                     VSGQSVRLEFPSDRYPETAKHIKEAISSGKSPVCTIDREGADHNRELSLKGVPTKKGKD
                     RDEWPMAMCSEGGEGADIKYISPKDNRGAGSWVGHKLDDYADGTKVEFIVK"
     gene            complement(3759..4211)
                     /locus_tag="NWS42_RS19740"
     CDS             complement(3759..4211)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019687441.1"
                     /locus_tag="NWS42_RS19740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_263560045.1"
                     /transl_table=11
                     /translation="MKFKNTAIILTGLLALTAVAPLAASATPFMGVELPKGHGGIVIQS
                     TGQEVHEVLGGTSPTMEFTVKKGYGHVKIKVKNTGNNTISWSLQGNGREYLNKTVGKGD
                     TLTWTSLNSYPNGMPSGEYVIQFRAGGNKVEGEAWGTTGQYPSDIN"
     gene            4455..5870
                     /locus_tag="NWS42_RS19745"
     CDS             4455..5870
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016822007.1"
                     /locus_tag="NWS42_RS19745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_263560046.1"
                     /transl_table=11
                     /translation="MKHTPTIRAELDSYLEQKGLSLAQFGQLAGMNRGIISAIVSGNKS
                     MSVNQLDLITEAMGLPEGEFYDLFIENYIIDHPPNMRRIEPFLFRCAELGKLDAIRRVL
                     GAIMDNLLYSPKLFEIAEELLSQGRNEAALLLYEGVAETERYQHSERLAVCQYRIFTIQ
                     VGDDQTQNLHAAAILEAFVERLNEIDQLDALKDLANLYRSLNKWSKVDEMARKMREKAE
                     IQYTIKHQQKKRECDELAKKLSRPLFVYITYADLLCASVCEAQGDYQQALEYTYAYTNL
                     DWVLETDEDTLYWLNLYKDWAQCNSYIYKLLSGDTSVLPDYVEYIAKSKVDKEMVTKIL
                     NVLLAANQYQLDIDDILRRFETIINSFTQKMQPSSDMYTSQVIPEQYARFGYELAYYYL
                     YRGSYGDGFKHLMYAMVQSHIINNETYFINCMGLFVKFRTHAVPEFKEEFLNLIEKVWL
                     NNVEKNGSSDRRS"
     gene            complement(6009..6872)
                     /locus_tag="NWS42_RS19750"
     CDS             complement(6009..6872)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014599900.1"
                     /locus_tag="NWS42_RS19750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA ligase family protein"
                     /protein_id="WP_263560047.1"
                     /transl_table=11
                     /translation="MFISPMLLETAAAPFSDSRYIFEPKIDGHRLIYSQQNGIVRLFTR
                     HNNDCTRQYPEIASVTFPHDIVLDGEIACVDPTTGVSDFESVMSRFQARKTDKITKLVA
                     ELPATFVVFDVLSFKGEDLRSLPLMERKSILASLTLTSPSFGVVPYIEGAGEALFEQIE
                     ARGMEGVVAKRKESTYVSRRSTSWQKVINWSYADVYITGYRKEEFGWLAGVPTSNGDKI
                     RPAGIIEFGASPTHKKAFYGVSKALITGEDREYVYVEPRIRARVKMRNWTKAGLLRSPV
                     FTEFIV"
     gene            complement(6978..7181)
                     /locus_tag="NWS42_RS19755"
     CDS             complement(6978..7181)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018673201.1"
                     /locus_tag="NWS42_RS19755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_263560048.1"
                     /transl_table=11
                     /translation="MKYGPRKPNLNKRIAARTSLKRQIAHRAGIKMPRGWGWLRNPRKA
                     AYNKVYNRSTFDIFKLLSKLFK"
     gene            complement(7192..7518)
                     /locus_tag="NWS42_RS19760"
     CDS             complement(7192..7518)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674958.1"
                     /locus_tag="NWS42_RS19760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="WP_254628520.1"
                     /transl_table=11
                     /translation="MALPYTIQIRPMNDESGHYFFATVAELDGCQSDGDTFAEAYESVR
                     EAMEGYLSVKLEYGDPIPEPPGEDQFSGKFNVRVPKSLHRQLSERAAAENVSLNQYCLY
                     KLSK"
     gene            complement(7603..7830)
                     /locus_tag="NWS42_RS19765"
     CDS             complement(7603..7830)
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674959.1"
                     /locus_tag="NWS42_RS19765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_174820713.1"
                     /transl_table=11
                     /translation="MARIDKIIEKMKNRPNGIRFEEAEKVLEHYGYIKARHDGTSHCQF
                     RNDKGDLITIKKKNPLKAGYVKDVLGRIGE"
     gene            7954..8226
                     /locus_tag="NWS42_RS19770"
     CDS             7954..8226
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007428550.1"
                     /locus_tag="NWS42_RS19770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_263560049.1"
                     /transl_table=11
                     /translation="MLPDIERKLLRILFNFFRQKLRMPTMLELEVKTGRSVEDIRTGLL
                     ALEQDNYITWEDQSDTRHIVIIEGWERGQSRPMSAGDASRYFTEY"
     gene            complement(8266..8775)
                     /locus_tag="NWS42_RS19775"
     CDS             complement(8266..8775)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635025.1"
                     /locus_tag="NWS42_RS19775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Ltp family lipoprotein"
                     /protein_id="WP_263560050.1"
                     /transl_table=11
                     /translation="MKKPFYKKWWFWVIIVVLVIGALGSKNNDPAKTATTDSKTTDTKA
                     VSTTANVEKKEEPKVKTEAKEESVPREHKAALQKAMEYAETMNMSKAGIYDQLTSEYGE
                     HFDKKAARYAIENIVFDWKGNALKKAQSYAETMNMSDSAIYDQLISEHGEKFTKEEAKY
                     AIANLK"
     misc_feature    complement(8773..8784)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    8884..8897
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 22.07"
                     /tool="antismash"
     gene            8937..9272
                     /locus_tag="NWS42_RS19780"
     CDS             8937..9272
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686529.1"
                     /locus_tag="NWS42_RS19780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YolD-like family protein"
                     /protein_id="WP_263560051.1"
                     /transl_table=11
                     /translation="MSKKLEGNGLWESSRIIIPEHKEAYLKLMKDRQRRGKPELDDQEV
                     QLIEQALIDSYNTRTAVTVTVFSPFDDTDMTGVVTSINTARREVKLFRGEDDYSWIKLE
                     DIISVNA"
     gene            10001..10531
                     /locus_tag="NWS42_RS19785"
     CDS             10001..10531
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /locus_tag="NWS42_RS19785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_263560052.1"
                     /sec_met_domain="AgrB (E-value: 1.6e-17, bitscore: 61.7,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLERLSRRIALGIKRADPEGPGSVEVLTYIIGIKLNWFSGLVLTT
                     LFGWMLGHMVGALVSFFSFVALRKFSGGLHFKSLTVCAVFSAALFASIPLVDLGHGGTL
                     LLTAITALVVLCVAPNNFEEINPSKIDPYLKWISLTIVLTNFIIQSPIIALSFAAQAIL
                     LLPLPILKGGERA"
     gene            10656..11372
                     /locus_tag="NWS42_RS19790"
     CDS             10656..11372
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /locus_tag="NWS42_RS19790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="WP_263560053.1"
                     /transl_table=11
                     /translation="MDNREIFAPEIMSDGSYGDRFSFFEGDLIAIENWKPKSNYEIPFF
                     FTTSGNYTVPTTLGEFSNGFPDFISLDSGNLVNLKNVIAHETADYGGKVFFGGSQTYTS
                     VNKTNSVFLGDLIDAAKKRPDEQRFILGETKTSVGLYPAKDVCFFDMWDPKKNYHVPRF
                     HNQNEYIAIILTFKKCKKVFPYLFPATPSHLINLSKVSGYDEQSYGTEITFKGTDYTCP
                     ISKSKLDMLKKILDIN"
     misc_feature    complement(11440..11455)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.98"
                     /tool="antismash"
     misc_feature    11538..11551
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.98"
                     /tool="antismash"
     gene            11720..11968
                     /locus_tag="NWS42_RS19795"
     CDS             11720..11968
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552281.1"
                     /locus_tag="NWS42_RS19795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_263560054.1"
                     /transl_table=11
                     /translation="MIQVKRVTTIMEACERSEDLLHKRLAVILNSLHDDYNVGVDIIEE
                     IEDLYIVKGDIYCRTGYQQGLKDALKPRGDVNTLTDC"
     gene            complement(12382..13578)
                     /locus_tag="NWS42_RS19800"
     CDS             complement(12382..13578)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369643.1"
                     /locus_tag="NWS42_RS19800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_263560055.1"
                     /transl_table=11
                     /translation="MTKRNNLLIFILTIGVFGILNTEMGVIGILPDIAEHFQVSISKAG
                     WLVSIFALGVAIAGPTMPLLFSGIDRKMVMLLVLGVFLMGNIVSIFTSSFTVLLITRVI
                     PAFFHPIYCSLAFTVAADSVSKEEAPKAVSKVFIGVSAGMVVGVPIVSFLNNAVSFEMA
                     MAFFAIVNALVFIATLLFVPSMPVKEVLTYGEQLSVLKKLNTWISIAAVIFINSAIFSI
                     YSYFAEYLQTVTKMSSNTISLMLFIYGGANIIGNILAGKLLVKSAIKSVVTFPFVLGAV
                     YIILFFTGQFTIPMAIITLIWGILAGIGGNINQYWITSAAPKAPDFANGLFLSSCNIGT
                     TIGAAVGGLFISEMGTQYVILVGLLSLILTTVFILLRNYMCSNTNGQAPIFIDTEKSP"
     gene            complement(13658..14014)
                     /locus_tag="NWS42_RS19805"
     CDS             complement(13658..14014)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NWS42_RS19805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_263560056.1"
                     /transl_table=11
                     /translation="MSVGEGVHDFTPGDRVVYVESPGAYAEEHIVPAHFAVRLPDAIDF
                     EAAAAMMLKGLTAQYLLRRTFRVEAGHTVLVHAAAGGVGSILTEQINLIPVLSLKIKLD
                     CYHNGHDESVRCYL"
     gene            complement(14011..14223)
                     /locus_tag="NWS42_RS19810"
     CDS             complement(14011..14223)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /locus_tag="NWS42_RS19810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alcohol dehydrogenase catalytic domain-containing
                     protein"
                     /protein_id="WP_263560057.1"
                     /transl_table=11
                     /translation="MKVILVRNHGGPEVLNYKDVEIRQPGPGEILARNRAVGVNYTDVY
                     MRTGSLDLESTILPFIPGKEGAGEV"
     gene            complement(14269..15225)
                     /locus_tag="NWS42_RS19815"
     CDS             complement(14269..15225)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394434.1"
                     /locus_tag="NWS42_RS19815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_327196933.1"
                     /transl_table=11
                     /translation="MLSSMSNYIFELSDNVTRKSVSYNNRFGIKIAADLYLPKNFNESK
                     KHKAVIIGAPYGGVKEQGSGIYAQNMAERGFVALAFDPSFNGYSSGEPRHLSSPDLFVE
                     DFSAAVDYVGTRSFVDRNQIGVIGMCGSGGFAISAAQVDRRIKAVATVSMYDISRAQRN
                     GFKDSLTEEDRNRILDEIAEQRYAEFEGNTPVLTQRGAAIGFDEHTDPIGREFGEFYST
                     PRGYHHNSLTQFTVTSSMSFMNFPLLTYIKSISPRPILFIIGEHAHSRYFSEDAYELAA
                     DPKELYIVLNAGHVDLYDKTNLIPLDKLESFFNENLK"
     gene            15373..16281
                     /locus_tag="NWS42_RS19820"
     CDS             15373..16281
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019390986.1"
                     /locus_tag="NWS42_RS19820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_263560059.1"
                     /transl_table=11
                     /translation="MEFRVLRYFLTVAREGSITGAADFLHVTQPTLSRQLKDLEQELGK
                     KLFIRSSHSIVLTDEGMLLRKRAEEIVDMVDKLEAEFRSMEETISGDIYIGGGETDAMK
                     QIARVVKDLQLSYPNIRYHLFSGNEDDVTERLDKGLLDFGILIQPADLSKYNYINIPAK
                     DVWGVVMRKDSPLAFKDTIQAVDLLNVPLICSRQAMQQTLSKNEFVHWFGEDFDKLNVV
                     TTFNLAYNAAIMVEEGIGYAITLDKIVNTSSDSNLCFRPLEPRLESGLNIVWKKHQVFS
                     DAADMFLNEIQAKFSDYLSDV"
     misc_feature    complement(16773..16787)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.47"
                     /tool="antismash"
     gene            16924..17547
                     /locus_tag="NWS42_RS19825"
     CDS             16924..17547
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686535.1"
                     /locus_tag="NWS42_RS19825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE family translocator"
                     /protein_id="WP_263560060.1"
                     /transl_table=11
                     /translation="MELQTILAFLGAAIVLTIMPGPDNVFTLTQSIAKGRNAGIFTTLG
                     LCTGLLVHIFAATFGLAAIIYQSSVAFAVIKYAGAAYLLFLAYKSFRAKDMSFELKSGD
                     TLNYKSLYKKGVIMNVLNPKISLFFLAFLPQFVNQESGNVSIQMLVLGVVFLIQTLVLF
                     ILISIFAGKVGSFLRRNPSFSKKINYIQGSLFTLIGLKIAFTQK"
     gene            17798..18523
                     /locus_tag="NWS42_RS19830"
     CDS             17798..18523
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686536.1"
                     /locus_tag="NWS42_RS19830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="collagen-like protein"
                     /protein_id="WP_327196934.1"
                     /transl_table=11
                     /translation="MFRHYCWQKKMHRRKFRRCSVKKFKVLPKVIVKGIQGPQGPQGPQ
                     GPQGLPGPQGVPGAQGQQGLQGFQGIPGPAGPVGATGPAGAIGPAGPAGPAGATGPAGP
                     AGVGISDFLSVFRADPGTGTDTVPGNSPITFTGVLANVGGAFTFTPPSPTITINETGSY
                     FISFSIHNQGNADFNLTVNGTPITPLPFSGNGGGPIAAEVIITVTTVPTTIQLVNANAT
                     PAQLHNNINTTMTILKLTP"
     gene            18616..18861
                     /locus_tag="NWS42_RS19835"
     CDS             18616..18861
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022133.4"
                     /locus_tag="NWS42_RS19835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phage terminase small subunit-related protein"
                     /protein_id="WP_263560062.1"
                     /transl_table=11
                     /translation="MEMPRERIPERDKSRQMWLESSGAMRLKDIAAALSVPEKSGSQRE
                     VYEPLVGDFDYFLLSKKHYLQKSYFQKILKIVRVAH"
     gene            18947..19570
                     /locus_tag="NWS42_RS19840"
     CDS             18947..19570
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821192.1"
                     /locus_tag="NWS42_RS19840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE family translocator"
                     /protein_id="WP_263560063.1"
                     /transl_table=11
                     /translation="MNDILAFTILLIFIVMSPGIDFALITKRTLTDGKKDGFKIALGLT
                     SGALVHTLAAALGLSIILMKSAVAFEVVKYAGAVYLIYMGLSSFIKKGKSNLVQEKQEN
                     SRKSAFTQGLVSNAFNPKVAIFFLTFLPQFVLPGYNPSLQFLLMGSLYALLSIIWFTTI
                     VLLLSYIRRWLMSPKVQNMIDKVTGLVLIGFGLNMIFKVQKTGS"
     gene            <19635..19709
                     /locus_tag="NWS42_RS19845"
                     /pseudo=""
     CDS             <19635..19709
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022133.4"
                     /locus_tag="NWS42_RS19845"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="phage terminase small subunit-related protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="ERHKAKQMWLERYGHMKLKDIAAS"
     misc_feature    complement(19777..19791)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.96"
                     /tool="antismash"
     misc_feature    complement(20469..20483)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.18"
                     /tool="antismash"
ORIGIN
        1 tgttaaagag cgaaccacga ataacaaaca gtggtttcag ttcctcacgt ttgtgttggg
       61 tgggaccata atcgctatcg ttacggcggt atttgtaaaa gggggattgt gataattgtt
      121 taatccaaat aaatatacga tagaacggcg ctacataaac aagcgccata acgtaagacc
      181 gggtactcgc ctaacaagcg ggaatccggt ctttttagtt gcacacgaca cgggtaatcc
      241 aggagcgtca gcggataacc attacaccta tttcaatggg ctggtagatc ggtctgcttc
      301 agcacaaacg ttcatagacg acaaaaagat tcttgaaatt atcccgactg gtaccggacc
      361 cgatccagca gaaaaggcat ggcacgtctt atacaacgta accacggata acgaattgta
      421 cggtgacgat gcgaacgata tagctttggg cgtggaattg tgctttggcg gcaagattga
      481 cacgctggag gcttacaaac gctttgcatg gtatctggcc tactgctgca acaaatgggg
      541 gattaaccca cgtactcaca tcccatcgca caagcaactg gacccggccc gtaagaggga
      601 cgtagaccaa gcccttgcga caatcggcaa gacgctcaag gaattggtct atgacgtgga
      661 aacggagctt aaaggcgctt ctgtggctcc tgctgcgctg gacttcacgc cgttgccagt
      721 ttatattgct cagtcgctta tagacaacta tgtatctcca gcttggtttg ccagccagaa
      781 ggcagcggat caggaaggca agacgcattt tcacaaccta gccaataacc ttcgggcggc
      841 ggccggactt aatgaaaaga atctgccact tgctggagct gtgaagttgt ttaaatccaa
      901 tgcacaagag attatcttcc ggtggcttca acctgcttgg tttaaggcta aacaggtagg
      961 cgataaggaa caaatgcaac attataacaa tctggccaac tatctgcgtt ctgctgcagg
     1021 tttgccaaca caataagagg gggatttata aatgcaaaac gtaatcgaaa caattcaacc
     1081 agctattagt accattgtta cagccattgt aggcgtactg gtgacctttg ctttggcagg
     1141 gattaacaca cttaagaata aggctaataa ttggctagat gcacgtacaa cagcggcaca
     1201 gcgagaggta atccataaag tagcagggga agcttttgca cttgctcaga ctacgtttaa
     1261 agaagctggc gggatccgca agatgcaaga ggctttacag tatgcatcct tgaggcttac
     1321 agaacagggc attgtggtat cgcctacgga gttgcaagcc gcgattgaga aagcatacct
     1381 ggagtataag gccaaaacaa aagcggtact cgctactgaa gcacagccaa atgaggaggc
     1441 agcacaagta gcagctaagg aagctgtatc aggtctggct gcaaagttaa atgatttctt
     1501 ggcacaggct acagcggagg tatcattagg tcaggttcag actgcacctg agcttattcc
     1561 ggcaccaaca gaatctgaac aagctcctgt tactacggag taagaccaat acagctacac
     1621 tagatacaca ttagaagccg atcagactta aagtttttta ttgaatatta ctcttttgga
     1681 gtgcataatg ttattatcaa aatgaaacgg aggtaccgaa ttatgagttt aaataagtat
     1741 gaaattcttc atcatatctc aaaattaggt accttcatgt ttctctatgg tttaatatat
     1801 gtaattcagg atttgatttt tggaaagttc caaatactta atgattatct tataaaggga
     1861 ctgcccataa aatttgtaat gttctctttt gtgagtatat ttattatcgc aaaatttatt
     1921 aaaataggtt ccccatacaa gaaaactcga tcatgactga tcgagttttt ctgttagttc
     1981 ttttggtagt tctgctttca atttatcttt atatatgcta tgttgctgct taatctcttt
     2041 cagtttgtgt tcatctttat gtttgatgaa cttgaatact ttttcgaaca agccttcact
     2101 ttgtaactcc acttcatttg aactttcttt ttttccattt gagttcttaa atttaaacga
     2161 gccacccaca agccctacta caaccatccc caagatcatc atcacataag gagactgaga
     2221 tataaattcc atcaatcctg gtgattgtac atttaatttt aagtctacat cttctttctt
     2281 gaaagtttcc tctggttttt ctgggttttc aattaaaggt actatatcaa gtaaagtact
     2341 tacgtatgat atcatgtcga cggcgggtat ttgtttttcc tgttttacat caacaactaa
     2401 gtgagctttg tcgcctttaa ggtagaaaga atgtaatgtc cgatcaatat aattcgcata
     2461 gtcattggcg ttgcttatag cttgatgagc ctgcatcata cggtaaagat acggatcgag
     2521 cctatctctc tgaacggttt tgatccaatt cacatcacgt cttttaatga atggacaaag
     2581 ccccatatct aaacctgttt cactgacttc ctcaatgtaa ggctctgact caatgatacc
     2641 gaaactaata tgtgatgagt tacttgaagg aatcattacg atgtcgttaa cgtttaactc
     2701 agacaagaaa cgataaatct ggccatatat tcttccaggt tggggaggct tctccccttc
     2761 gtctggttta tatgtaatgc taattaaatc ggtcatctcg tctttctcag tgtttaagaa
     2821 tttctcaata tcagaaaatt cattccagcc tatcccaata aatccatcat ggaaaaactc
     2881 atcatagaat tcaccgcttt gcgttcttac cagccaatat ctcctatctt caggtatcat
     2941 tacaatggga atttcatttt cttttataaa acgcattaat ccttccaaaa agatcccccc
     3001 taaaaatgtc gataaataga gtttaaatga attatgagaa accatcaaca atgttatgtg
     3061 aagatttgaa aagttaggat agatgtagta atatacaaac aaatccgaaa gcatcggtga
     3121 caccctactg gctaagctgg tggggtgtat ttacgtttag ggggagacgt tatgcgtaaa
     3181 acatgtatta caatttgctt gcttggggcg ttaatagtat ttggtggatt tataaataca
     3241 ctgtcccacg cagaatgggg cgataagccc cgtgtacaaa cctcagctcc tgtatcagct
     3301 acaacagtgt ctgggcaatc cgtgcgttta gaattcccgt cagaccgtta tcccgaaaca
     3361 gcaaagcaca ttaaagaggc tatatcatcc ggaaagtcac cagtatgcac catagaccgt
     3421 gagggggcag accataaccg tgaactatct ctgaaaggtg tacccactaa aaagggtaaa
     3481 gaccgcgatg aatggcctat ggctatgtgc tccgaaggtg gagagggtgc agacattaag
     3541 tacataagtc caaaggataa tcgtggagct ggatcgtggg taggtcacaa gctggacgac
     3601 tacgctgacg gaacaaaagt tgaatttatc gttaaatagt gaaaagccca gctaatccac
     3661 taaggttagc tgggcttttg ctagacacta agtatggcaa cattgtattc ataaagagtc
     3721 tcggatatta atccagatac tcccctgctt gagattggtt agttaatgtc tgatgggtac
     3781 tgaccagtag taccccaagc ttcgccttca actttgttac cgccagctcg gaattgaatt
     3841 acgtattccc ctgatggcat cccgttaggg tatgaattaa gactagtcca cgttaatgta
     3901 tcacctttgc caacagtttt attaaggtat tctctcccgt taccctgtaa gctccacgaa
     3961 attgtgttgt ttcctgtatt cttcacttta atctttacat gtccataacc ttttttgaca
     4021 gtaaactcca tcgtcggact tgtcccgcca agaacctcat gtacttcttg accagttgac
     4081 tggataacta tacctccatg cccctttggt aattcaaccc ccataaatgg tgtggcggaa
     4141 gcagccagcg gagctactgc cgtaagagcc aatagacctg taagaatgat tgctgtgttt
     4201 ttaaacttca tcaaaaccgt cctcccgaat ggtataattt gtactactga ccaatcatag
     4261 cacataaaaa ggaataaaat gtacttttat attaacaaaa tggtaaaaaa atattaatat
     4321 tcgaaaatgc atatttcatt tgaatattgc atgacaaata tttcaaaact tattgacaac
     4381 tgttaaatta tgaacaacaa tagattgcaa taatataaaa gttttggaat ttctgtatga
     4441 agggtggata tgacatgaag catacaccta cgattcgagc agaattagat agttatctag
     4501 aacaaaaggg tttgagctta gcgcaatttg gacaacttgc aggcatgaat aggggaataa
     4561 taagtgctat tgtgtcaggg aataagtcta tgtcagttaa ccaacttgac ttaatcactg
     4621 aggctatggg tttaccagag ggagagtttt acgatctgtt tatagaaaac tacattattg
     4681 atcatcctcc aaatatgagg cgaatcgagc cgtttttgtt tcgctgtgcg gagttgggca
     4741 agttggatgc aatccgtcga gtactgggag ccatcatgga taatctgcta tattcgccca
     4801 agctatttga aatcgcagaa gaattattgt cgcagggacg aaatgaggct gctttgctgc
     4861 tttatgaggg tgtagctgaa acagagagat atcaacattc tgaacgcttg gcagtctgcc
     4921 aatatcgtat attcacgatt caagttggag acgatcaaac ccaaaatctc cacgctgctg
     4981 cgatacttga agcttttgtt gagcgattga atgaaataga ccaacttgac gcattgaagg
     5041 acttagcgaa tttgtatcgt tcattgaata agtggagcaa ggtcgatgaa atggcaagaa
     5101 aaatgagaga aaaagcggaa atccaatata caattaaaca tcaacagaag aaacgagagt
     5161 gtgacgaact tgctaagaag ttaagtcgtc ccttgtttgt gtacatcaca tatgccgatt
     5221 tactatgtgc aagtgtctgc gaagcccaag gcgattatca acaagctcta gaatatacat
     5281 atgcctacac taatttagat tgggtattgg agacagacga agatacttta tattggctaa
     5341 atctttataa ggattgggcg caatgtaata gttacattta taaacttttg tccggtgata
     5401 caagtgtgct tccagattat gttgaataca ttgccaaatc aaaagtagat aaagaaatgg
     5461 taactaagat attgaacgta ctgcttgcag ctaaccagta tcaactagat atagacgata
     5521 tacttagacg ttttgaaact ataattaatt cgtttactca gaagatgcag ccatcatccg
     5581 acatgtatac tagtcaagtt ataccagaac aatatgcacg atttggatac gagttagctt
     5641 attattattt atatagagga tcatacggtg atggctttaa acatttgatg tatgcaatgg
     5701 tacagtcaca tataataaat aacgaaactt attttataaa ctgcatgggg ctattcgtga
     5761 agtttagaac acatgcagtt cctgagttca aagaagagtt tttaaatctt attgaaaagg
     5821 tgtggttaaa caatgttgag aaaaatggtt cttctgatcg tcgcagctag ttttttattt
     5881 attgtgagtg ggcctaatca tgcgtatgac cagcacccta ttcagcttaa tgctaccgtt
     5941 ggaggttcct aagcttagat aaacaaaaaa agctctgcta acctcgcggt cttgcagagc
     6001 ttttcttgct atacaataaa ctcagtgaac accggactcc gcagcagccc agccttagtc
     6061 cagttacgca tctttacacg agcccttata cgtggttcca cataaacata ctcgcgatcc
     6121 tccccagtta tcagcgcctt agacacacca taaaacgcct ttttatgagt tgggctggcg
     6181 ccaaactcga taataccagc tggacgtatc ttatcaccat ttgacgttgg aacacctgcg
     6241 agccatccga attcctcttt gcgataaccc gtaatgtaca catcagcata ggaccagtta
     6301 atcaccttct gccaagacgt tgatcgacgg ctgacatacg tactctcttt acgcttggcg
     6361 accacgcctt ccatgccgcg cgcttctatc tgctcgaaca acgcctcccc ggcgccttca
     6421 atatacggaa caaccccgaa actagggcta gtaagcgtta gactagcgag tatggacttg
     6481 cgttccataa gcggaaggct ccgcaagtcc tcgcctttaa agctcaagac atcgaagaca
     6541 acgaaagtag ccggtaattc agctacgagc ttagtaatct tgtccgtttt cctcgcctgg
     6601 aagcgggaca taacggattc aaaatccgat acacccgttg tcggatcaac gcacgcaatc
     6661 tcaccgtcca acacaatgtc atgcgggaat gtaacgctag ctatctccgg gtattggcgt
     6721 gtgcagtcat tgttatggcg cgtgaataag cgtacaatgc cgttctgttg cgaatatatt
     6781 aagcggtggc cgtctatttt cggttcgaag atatagcgcg aatccgaaaa gggggcagcc
     6841 gctgtttcaa gtaacattgg agaaataaac aaatagacca cctcagatta attataacgc
     6901 tcatttatgc agtagtgagg cggtaagtat tggagaggaa aagccctgct aaccttaatt
     6961 ggtcggcaga gcttttttta tttgaacaat tttgaaagca gcttgaatat atcaaacgtt
     7021 gaccgattgt acaccttatt gtatgccgcc ttacgtgggt tacggagcca tccccagccc
     7081 cgtggcatct tgatgcctgc acggtgcgca atctgccgtt tgagactggt acgagctgct
     7141 atacgcttgt ttaggttggg cttacgtggg ccatatttca ttctctgtcc ctcatttgct
     7201 tagtttgtat aagcaatact ggttcaggga aacattttcg gcggctgctc tttctgatag
     7261 ttggcggtgt agactcttgg gaacacgaac gttgaacttg ccgctaaatt ggtcttctcc
     7321 tggtggctct ggaataggat cgccgtactc aagtttcaca gacaagtaac cttccatagc
     7381 ctctctaacg ctctcgtatg cttctgcaaa ggtgtctccg tcactttggc aaccgtctaa
     7441 ctctgctaca gtagcgaaga agtaatgtcc gctttcatcg ttcattggtc taatctgtat
     7501 agtgtacgga agcgccatgt aatacgctaa atctttatgc tgattattat ttgccattat
     7561 atatcggcta ggatatattg aggattaagg aaaggggagc ggtcactccc caatccgacc
     7621 cagtacgtct ttgacgtacc ccgcctttaa tgggtttttc tttttgatgg tgattaggtc
     7681 tcctttatca tttcggaatt ggcaatgtga cgttccatcg tgtcttgcct ttatgtatcc
     7741 gtaatgttct agtacctttt cagcttcctc aaatcgaatc ccgttagggc ggtttttcat
     7801 tttttcgatg attttatcta tcctagccat gtctcacctc ctgtatatat agtaccatat
     7861 atagtaccat tatgcaaggg gttaatggaa aaatattctg agttttacga gaacgtgcgt
     7921 ttgtatataa tgataacaaa ggagtggatc gctttgttac cagacatcga acgtaaacta
     7981 ttgcggatat tgttcaattt ttttcgtcaa aaacttagaa tgccgacaat gctggaattg
     8041 gaggtcaaga caggtaggag tgtagaggac atcagaaccg ggctgctggc tttggaacaa
     8101 gataactata ttacttggga agaccaatca gatacgcggc atatcgtcat tatagaaggc
     8161 tgggaacgtg gacaaagcag acctatgtcg gcaggggatg ccagtagata cttcacggaa
     8221 tattaaaacc ccggattgct ccggggctgc tattgtccat ttttgttatt ttaaattagc
     8281 tattgcgtat tttgcttctt ccttagtgaa tttttccccg tgttctgaaa tcaattgatc
     8341 gtaaatagcc gaatctgaca tattcatagt ttctgcatat gattgtgcct ttttcaatgc
     8401 gtttcctttc caatcaaaca caatgttttc aatagcatat cgagcggctt ttttatcgaa
     8461 atgctcaccg tattcagaag tcaattgatc gtaaatgcct gctttcgaca tgttcatagt
     8521 ttctgcgtat tccatcgctt tttgcaatgc ggctttgtgt tccctaggga cactttcctc
     8581 ttttgcctcg gttttaactt taggttcttc tttcttttcc acatttgctg tagttgacac
     8641 tgctttagta tctgttgttt tagaatcggt tgtcgcagtc ttcgctgggt cattgttctt
     8701 cgaaccaagg gcacctatga ctaatacaac gataataacc caaaaccacc acttcttata
     8761 aaacggcttc ttcaaaacgt tttcccccta attaaccaat attattataa aaatagtata
     8821 tatgacctag gaatattttt ctagtacgaa agttttatag atagcaaata aaatatcttg
     8881 ttaggaacaa atgttcgtaa tataatcgtt acataataca tcaggaggct acatacatgt
     8941 ctaaaaaatt agaaggtaac gggttatggg aaagcagtcg gattatcata ccagaacata
     9001 aagaggctta tctgaagctc atgaaggacc gacaacggag gggaaagcct gagctagacg
     9061 accaagaggt acaattgatt gagcaggctt taatagactc ttacaataca cgtacagccg
     9121 tcacggtgac cgtatttagc ccgtttgacg atacggacat gactggtgta gttacgtcaa
     9181 ttaacaccgc tagacgcgaa gtaaaactgt ttcgcggtga ggatgattat agttggatta
     9241 agctagaaga tattatttcg gtaaatgcgt aaaatacctt gattacacaa tacaccgcta
     9301 tataattaag gtaacttaaa tatagggtgg tgtatggtgt taagtaatgc ggtttatata
     9361 ttacttggta ttttggatgc aatagccgtt atggtgttta gtcttaaaat atatatgctt
     9421 ccagtagcat catataaaaa aagaattatt ggttttgggg tattaatcgc tataatttct
     9481 tttttcatgc gtgtggtttt cgacattcct aagtgggatt tacctttaca atatatactg
     9541 tttatattgt tttttagata cgggatgtct attaagacgc atctagcggc atttatcctc
     9601 ggagctggaa ttagtgcata tgcgattgtg caagtcggcg tctactatct ttataacttt
     9661 ctgggtattt tgcagaccgg ggtgctacag caaaacacaa cttatcaggt ctacatctta
     9721 caaataacat atatccttat ctgttttgct atatcgttta tccttagaat ttattatacc
     9781 ggtttctcgt ttatcgttac tccgccgcac gattttatat ggaaagaaaa ttatttatcc
     9841 gaagaaaata ggagggtatc agttagtgct cttttatcct ctgtcaccat cttcatagca
     9901 gaggttctat tataccagtc gagtcctttt gttcttttgc ttatgtctat attcaccttc
     9961 ggtttctcct atattttgtc aagaaaggag gacagggaag atgctagaaa ggctatcgag
    10021 gcgtatcgcg ctaggaataa aaagagctga tccagagggg ccaggaagcg ttgaggtgtt
    10081 aacctacata ataggtataa agcttaattg gttttctggt cttgtgctta cgacgttatt
    10141 tggatggatg ctaggacaca tggtaggtgc actcgtatct tttttttcat ttgttgcact
    10201 taggaagttt tctggaggat tgcattttaa atctttaacg gtttgtgctg tgttttcagc
    10261 tgcgcttttt gctagtattc ccttagtgga tttaggacat ggtggcacgc tactacttac
    10321 agccataaca gcgttagtcg tgctatgcgt ggcgccaaat aacttcgaag aaattaatcc
    10381 ttctaaaata gatccatatc taaaatggat ttcccttact attgtactca ctaattttat
    10441 tattcaatct cctataattg ctctttcttt cgcggctcag gctattctac tcctaccgct
    10501 accgattctg aagggaggtg aaagagcatg aaaaagacta ttgcgcgtct tgtgtccaaa
    10561 cagttagtaa aagatgctaa caaagctgct gtaagccgca aaacaatcat cggagcttta
    10621 ccgctgccga aagaactaaa gaaatgattt gatctatgga caacagagag atttttgcac
    10681 ctgagattat gtcggatggt agttacggag atagattttc tttttttgag ggcgatctaa
    10741 tagccatcga aaactggaag ccaaaatcca actatgaaat accgtttttt ttcacaacca
    10801 gcggtaatta tacagttcca actacactcg gggaattctc aaacggattt cccgatttta
    10861 ttagcttgga ttcaggtaat ttggttaatc taaaaaatgt tatcgcacac gaaacagcag
    10921 actatggcgg caaagttttt tttggtggtt cccaaactta tactagcgtt aataaaacta
    10981 attctgtatt tttgggtgac cttattgatg cagctaaaaa gcgacctgat gaacagcggt
    11041 ttattttagg cgaaaccaaa acttcggtcg gcctttatcc tgcgaaagat gtttgctttt
    11101 ttgatatgtg ggaccccaaa aaaaattatc atgttcctcg ttttcataac caaaatgaat
    11161 acatcgcaat cattttgacc tttaagaagt gtaagaaggt ttttccttac ctgtttcccg
    11221 ctacaccaag tcaccttatt aatttgtcta aagtatcagg atatgatgag cagagttatg
    11281 gaacggaaat tacatttaag ggcactgact acacttgccc tatatctaaa agcaaacttg
    11341 atatgcttaa aaaaatatta gatattaact aagcccgcct ttggtagggc ttttatcata
    11401 catataggga aaagtcaaga taaattcacc ctaggatggc ccaactggca ttcgacatca
    11461 tacgacattt ttccccttta cgtcataatg tgatgttggt ataaaaaggt taagagattc
    11521 aagaactcaa aataacagga acgaaagttc ttattactag aaaggatgtg aaaaaacgcc
    11581 tcccatagga gaggcgctta atgtgggttg gcatccctgc caggatagcc aagttagatc
    11641 ccacggaaaa taagtcacag tgagtataca tgaaaaatga ttgtcagtaa atgcatcgga
    11701 agtcgggaga gtgaatggga tgatacaggt aaaaagagta actactatta tggaggcttg
    11761 cgagcgtagt gaggacttgt tacataaaag gcttgcagtg atcctgaaca gtttgcatga
    11821 tgattacaac gtcggtgtgg atattattga ggagattgaa gatttataca ttgtaaaagg
    11881 ggatatttat tgtagaacag gttaccaaca gggattaaag gatgctttaa aaccaagagg
    11941 ggatgttaac acgttaaccg attgttaacg caatacatta ttttcggttc ttattagttc
    12001 ataaataaca caacaaaaac cctttgtatt catatatttt cataaataac acagggggaa
    12061 gcggaatggg cggcatgatg taattagggc ctcaaaaccc tgtaataccg ctatttaaag
    12121 ggctcccgag agggagccct tttctataaa atcacataaa aatcacattc tctgcaatga
    12181 gtcagattta gagcatgtct gcgaagtaat ttttaatctt ctcattggca tttttcttca
    12241 ttttctccgc aacgtgtgta tagactttca atgtagtttt tgcgtcatca tgaccaactc
    12301 gcttcatgac cgttttgaga tcaacttggg aattcgccgg aagcattgct gcaactatac
    12361 tttccggaag gagcttatag cctaggggct tttttcagtg tctataaaaa taggggcctg
    12421 accatttgtg ttactacaca tgtagtttct tagtaaaata aacaccgtgg tcaatatcaa
    12481 cgataataac cctactagta ttacatattg cgtacccatt tctgatataa ataaccctcc
    12541 tacagctgca ccaattgtgg tacctatatt acaggatgat aaaaataagc cattagcgaa
    12601 atcgggtgct ttaggtgctg cagacgtaat ccaatattga ttaatgttac ctcctattcc
    12661 agctaatatt ccccagatta aagtgataat cgccataggt atggtaaact gtccagtaaa
    12721 aaataatatg atgtaaacag cccccaatac aaagggaaaa gttactacag attttatcgc
    12781 acttttcacg agtaatttcc ctgctaaaat gtttccaatt atattggccc caccgtagat
    12841 gaataacatt aaactaatag tattcgaaga catcttcgta actgtttgaa gatactcggc
    12901 aaaataacta tagattgaaa atatagccga atttatgaaa atgacagctg caatggaaat
    12961 ccaagtattt aatttcttta atacagataa ttgttctccg taagtaagaa cttccttaac
    13021 aggcatagac ggcacaaata gtaacgtagc aataaatact aaagcattta caatagcgaa
    13081 aaatgccatt gccatttcaa acgaaacagc attattaaga aaacttacga tcggtacacc
    13141 gactaccata cccgcagata caccaataaa tactttagag actgctttag gagcttcttc
    13201 tttgctaacc gagtcagcag ctactgtaaa agccaatgaa caataaatag gatgaaaaaa
    13261 ggctggtatt acacgagtaa ttaatagaac agtaaagcta gatgtgaata tggagacaat
    13321 gttccccatc aagaaaacac ccagtacaag caacataacc atcttgcggt ctatacccga
    13381 aaacaataac ggcattgttg gtccagctat tgcaacacca agggcaaaaa tgctcaccag
    13441 ccatcctgct tttgatatgc tgacctgaaa gtgttcagct atgtcaggta atattccaat
    13501 aacacccatt tcagtgttta agatgccgaa aactcctatg gtcaatatga aaattaataa
    13561 attatttcgt ttagtcaaaa aaacaactcc tacccttaat aaattgctcc gaaagaaaaa
    13621 ttctcccgac tcctcagttt gttcaataaa cgtgacttta caagtaacaa cggacactct
    13681 catcgtgtcc gttgtggtag caatctagtt taattttgag tgataggacc ggaatgagat
    13741 ttatttgctc agttagaatg ctcccaactc ccccagctgc tgcgtggacg agaaccgtgt
    13801 gccctgcctc cactcggaag gttcgtctta acaaatattg cgcggttagt ccctttaaca
    13861 tcatcgctgc agcagcttca aagtctatgg cgtcgggaag acgcacagcg aaatgagcgg
    13921 gcacaatgtg ctcttccgca taagcaccgg gcgactcaac atagacaacc cgatctcctg
    13981 gtgtgaaatc atgcacacct tcccccacac tcaaacctca cccgcaccct ctttaccagg
    14041 tatgaatgga agaatagtgg attctaaatc aagggaaccc gtacgcatat agacatcagt
    14101 gtaattgacg ccaactgcgc gattgcgcgc gaggatctcg ccaggaccgg gctgacggat
    14161 ctcaacgtct ttatagttaa gcacttccgg tccgccgtgg tttcgaacaa ggatcacttt
    14221 catgtcaggt tacccttctg ttagacctta tagcttttga agcgtttctt acttcaaatt
    14281 ctcgttaaaa aatgattcca atttatcgag tggaatcaaa ttcgttttgt cgtacaaatc
    14341 aacgtgtccg gcatttagga cgatgtacag ttcttttggg tctgctgcca attcataagc
    14401 atcttcacta aagtatcgtg agtgagcatg ttcgcctatg ataaacaaaa ttggccttgg
    14461 cgaaatcgat ttaatataag taagcaaagg gaagttcata aaggacatgc tgcttgtcac
    14521 ggtaaattga gtaagtgagt tatggtggta cccacgaggt gtagaataaa actcgccaaa
    14581 ctcacgaccg ataggatctg tatgttcgtc aaatccaatc gctgctccgc gctgggtcaa
    14641 tactggcgtg ttgccttcaa actctgcgta gcgttgctcg gcaatctcat cgagtattct
    14701 attacggtct tcttcggtta acgagtcttt aaagccgttg cgttgcgcgc gggatatatc
    14761 gtacatgcta accgtggcaa ccgctttaat gcgtctatcc acctgcgctg cgctgatcgc
    14821 aaaaccaccg ctgccacaca taccgattac accaatttga ttccgatcta caaatgaacg
    14881 cgttcctaca taatcaacag ccgcgctgaa atcttctacg aacagatcag gcgaagaaag
    14941 gtgccgtggc tctccgctgc tgtacccatt gaacgatggg tcaaatgcaa gagccacaaa
    15001 accacgctcc gccatgtttt gagcatagat accggagcct tgctctttta caccgccata
    15061 gggcgcacct ataataactg ctttgtgctt ctttgactca ttaaagtttt ttggcaagta
    15121 aagatcagcc gcaattttga ttccaaacct gttgttatat gatactgatt ttcttgttac
    15181 gttatcgctt aactcaaaaa tatagtttga catactagat aacatccttt cttaaatgtg
    15241 tgttttcacc cttcgttatt tattctattt atgcctgtag catataacaa atacttatat
    15301 ctcatattgt gatatgctta ttaggcattt ctaaaatagg tatactaaaa cataagaatg
    15361 gaggagtatt agatggaatt tagagttttg cgatattttc ttactgttgc aagagaagga
    15421 agtattacgg gtgctgctga ttttttgcat gttacgcagc cgaccttgtc cagacaatta
    15481 aaagaccttg aacaagagtt agggaaaaag ctgtttattc gtagtagtca cagtatcgtt
    15541 cttacagatg aaggaatgct cctgcgaaag agagcagaag agattgttga tatggtcgat
    15601 aaattagagg cagaattccg ttccatggaa gaaacaataa gtggtgatat atacattggc
    15661 ggtggggaaa cagatgctat gaaacagatt gcacgcgtag taaaagattt acagttaagt
    15721 tatccaaata tacggtatca cctctttagt ggaaacgaag acgatgtgac tgaacggctt
    15781 gataagggct tgcttgactt tggtattttg attcaaccag ctgatttatc aaaatacaat
    15841 tatatcaata ttccggccaa agatgtttgg ggcgttgtta tgagaaaaga cagtcctctt
    15901 gctttcaaag ataccattca agcagtggat ttattaaatg ttccgctaat ttgttcacga
    15961 caggctatgc aacagacatt gtctaaaaat gaatttgtgc attggtttgg tgaagatttt
    16021 gataaattaa acgtcgtgac aacattcaat cttgcatata atgctgctat tatggttgaa
    16081 gagggcattg ggtatgccat aacccttgat aaaatagtga atacatctag tgatagtaac
    16141 ctttgtttta gaccgttaga gccaaggctt gaatctggat taaatattgt ttggaaaaaa
    16201 catcaggttt tttccgatgc tgctgatatg tttttaaatg aaattcaggc taaattttca
    16261 gattatttat cagatgtata ggtcaagctc gtaaacgttt tataaattat tgaagataat
    16321 cactttctcc gaagatgcca ctaatcgggt attgtattat cgtgcaagtt cgttgcgtta
    16381 acctgccgtt cagaacaaca aaatggcagt agatcagaga ttccgctgcc cttttgttgt
    16441 tgctgaatca gcaactatcg tctattcatt tacttattcc agacatctca ttcactccac
    16501 agagaatcga gcaaataagg ccaccaggct gcgtccacct tttcttgaac tatcctcccc
    16561 gttagctaaa tgtcgccgtt cagtcaacaa gctttcagca ggcctccgta agttggagta
    16621 tattaaattc tcagaaatat cattgttgct tagtatgcct caataacaat agaaggaaat
    16681 tgttgatgag accgatatgt ggtgaataat tcgttatttg aatcacaaaa tattcacaat
    16741 ctcggcgaat atagatgaat ataggaaaat ggaattttct gaatagagaa caaaacatgt
    16801 tgctcaagca gcttacagca gagtttctgt aattcggcag tttacattca atcatttgct
    16861 ctaatagctt ctatacatat tgtggtgtag tatatatgca aattatccaa agggggaagt
    16921 tgtgtggagc ttcaaactat attagctttt ctgggagcag caattgttct caccattatg
    16981 ccgggtcccg acaacgtgtt cactctgaca cagagcatag caaaaggtag gaatgcaggg
    17041 atatttacta ctttaggcct ttgcacggga ttgttggttc atatttttgc agcaaccttc
    17101 ggcctcgcag caataattta tcaatcttcc gtggcatttg cagtaatcaa atacgctgga
    17161 gcagcttatc tattattttt ggcgtataag tcatttagag caaaagatat gagttttgaa
    17221 ctcaagagtg gtgatacctt gaattacaaa tcattgtata aaaaaggtgt catcatgaat
    17281 gtgttgaatc ccaaaatatc actttttttc ttggcttttc ttcctcagtt cgtaaaccaa
    17341 gaaagcggta atgtttctat tcaaatgcta gttcttggag tggtattttt aatacaaact
    17401 ttagtgcttt ttattttgat aagtattttt gctggaaaag taggctcctt tctacggaga
    17461 aatccatcct tttctaagaa aatcaattat attcaaggct cgttatttac acttatcgga
    17521 ttgaaaatcg catttaccca aaaataatag aaggtagcac tttttaaact aaccattaag
    17581 gactagctgc tgtgctagtc ctttttttgt ttgaaaagca tacaacacac gccgagaaca
    17641 tggtgggtgg tcacagaggt ttaagatcat gaccaacgag ttggagcata cggacagacc
    17701 tatgcctatc ttttcctaga tgaagtcaac agcccaatct ttttggttaa ttgcacaata
    17761 agctaatttg actagacttt ggaggtgacc attaattatg tttagacatt attgttggca
    17821 aaagaaaatg catcggagaa aatttagaag atgttcagtt aaaaaattca aagttttgcc
    17881 gaaggtcatt gttaaaggaa ttcaaggacc tcaaggacct caaggaccgc agggacccca
    17941 gggactgcca ggtccacagg gagttccagg tgctcaaggt cagcaagggc tacaaggttt
    18001 ccaaggaatt cctggccctg cagggccagt aggagcaacg ggaccggcag gagcaatagg
    18061 accagcagga ccagcaggac cagcaggagc aacggggcca gcagggcctg ctggcgtcgg
    18121 gatatcagac ttcttaagtg ttttcagagc agatcctggt acggggactg acacagttcc
    18181 gggtaactca cccattacct ttactggggt tttagcgaat gttggaggtg catttacttt
    18241 cactcctcca tctccaacga taacaattaa cgagacgggg agctacttca tctcattttc
    18301 tatccacaac caaggtaatg cggacttcaa tctcactgtt aacggaactc ctataacacc
    18361 cttacctttc tcaggtaatg gtgggggacc tattgcagct gaggttataa taaccgttac
    18421 aacagttcca actacaatac aactggtaaa tgccaatgca actcctgctc agttgcacaa
    18481 taatattaat acaactatga caatattaaa gttgacgcct taatattatt gcttagtagt
    18541 agcttattca gtaaaaggtt taaggtttat gtcttttatc cggtcagttt gtctaattat
    18601 tcagagacct gagatatgga aatgcctaga gagagaatcc ctgaacggga caaatccaga
    18661 cagatgtggt tggagagcag cggggcgatg cgacttaagg acatcgccgc tgctctttct
    18721 gttcctgaaa agtctggttc gcaaagggaa gtctatgaac cgctggtagg agactttgat
    18781 tattttctgt tatcaaaaaa acattattta caaaaatctt attttcagaa gattcttaaa
    18841 atcgtacgag tggcgcatta gcttcaaaag gatgaatgtt aggtattgaa atatagctga
    18901 taacgcgtta gtattgtgta tctgaaattt catctggagg cactttatga atgatatatt
    18961 ggcatttacg atactgctca tctttattgt catgtcacca gggattgatt ttgcattaat
    19021 aacgaaaaga actctcactg atggcaagaa ggatggtttc aaaattgcac taggtttaac
    19081 tagcggagct ctcgttcata cattggcagc cgcattgggg ctttctatca tattaatgaa
    19141 gtcggctgta gcatttgagg tagtcaaata tgcaggggca gtttacttga tatatatggg
    19201 gctgtcttcc tttataaaaa aaggaaagag taatttggtt caagagaagc aagaaaattc
    19261 tagaaaatca gcgtttacac aaggattggt ttccaatgca ttcaatccga aggttgctat
    19321 tttttttctc acgttcttac cgcaatttgt tctaccgggc tataatccca gtttgcagtt
    19381 tttactgatg ggctctcttt atgctctact ttcgattata tggtttacta caatcgtatt
    19441 attgttaagt tacataagaa gatggttaat gtcacctaaa gttcaaaata tgatcgataa
    19501 ggtcacggga ctcgtgctga ttggttttgg tttaaatatg atcttcaaag ttcaaaaaac
    19561 aggatcataa ataaaggaac gggctatgga agctcgttct tttttgttag gggagacgac
    19621 cagaaagaga taccgagcgg cataaggcaa aacagatgtg gctggagaga tacgggcata
    19681 tgaagctaaa ggacatcgcc gctagttgag ggttaaacgc cttaagtgaa agcgttgaca
    19741 taaatttcaa gcctctatat acttagctta gttaattgaa aatgaatagc actgggattg
    19801 ttactgttac gcaggcaaaa cctaaggaac caaatgctaa gatccaagga tcttatgtat
    19861 tttgttatct taggtttttt tgtttataga gacaggaagg ttaaatatgg cggtttcggc
    19921 aacctgttga agccgtttct tctattatga tgttcacatc tgagaggagc gacaaacttg
    19981 gaatacaaat atgagctagt ccaggcagac aatcattttc cattaaaaat tatagctcat
    20041 tcctccaatg aaccagcata catacctagg cattggcatg acagcatcga aatatcctat
    20101 gtactctcag gaagaatcga caatatctat atagatggca tgaattatag ctccaaagaa
    20161 ggagatattg tgctcattaa ttcgaatgct attcattcct tttctgtggc taagggccaa
    20221 ggtagacagg ctataactgt tctgattcct tctggattta ttcgagaaaa tataaaggat
    20281 agtaatccca ttttatttga ttgtatatcc attttcgaaa ccaacgagca gagattacag
    20341 cattttgagg agctgcgtga tctgttaaac acattatttg tggcttacct caaccagcac
    20401 aatgaccctt tagtacatat tcacttaacg tctctatctt atagattggt gtacctttta
    20461 ttaatgcatt ttaaaattga caacgaatcg ctccctggct ttaacacgaa taaatatttg
    20521 gagcggctga c
//
