LOCUS       NZ_CP102865            24068 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Paenibacillus polymyxa strain S3 chromosome, complete genome.
ACCESSION   NZ_CP102865
VERSION     NZ_CP102865.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 5740272)
  AUTHORS   Yang,S., Jin,D., Li,H., Jiang,L., Cui,J., Huang,W., Rang,J., Li,Y.
            and Xia,L.
  TITLE     Screening of new Paenibacillus polymyxa S3 and its disease
            resistance of grass carp (Ctenopharyngodon idellus)
  JOURNAL   J Fish Dis 46 (1), 17-29 (2023)
   PUBMED   36097971
REFERENCE   2  (bases 1 to 5740272)
  AUTHORS   Yang,S., Jin,D., Li,H., Jiang,L., Cui,J., Huang,W., Rang,J. and
            Xia,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2022) State Key Laboratory of Developmental
            Biology of Freshwater Fish, College of Life Science, Hunan Normal
            University, Yuelu, Changsha, Hunan 410006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-JUL-2021
            Assembly Method                   :: SOAPdenovo v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164.83x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:01:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,183
            CDSs (total)                      :: 5,026
            Genes (coding)                    :: 4,956
            CDSs (with protein)               :: 4,956
            Genes (RNA)                       :: 157
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 111
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 48 of 70
            Pseudo Genes (internal stop)      :: 10 of 70
            Pseudo Genes (multiple problems)  :: 17 of 70
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:25:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4280620
            Orig. end                         :: 4304688
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Hunan Normal University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..24068
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="13"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..24068
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..24068
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:14068](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..14068
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            242..1162
                     /locus_tag="NWS42_RS19220"
     CDS             242..1162
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821230.1"
                     /locus_tag="NWS42_RS19220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_102998578.1"
                     /transl_table=11
                     /translation="MQQATGQVPVIRMEGVSKIISSKAIVDNLTLEVPAGQVFGFLGPN
                     GAGKTTTIRMMVGLISISKGDIHICGDSINTDFEKAVSHVGAIVENPEMYKFLTGYQNL
                     QHFARMSPGVTQERIDEAIRLVGLGNRIHDKVKTYSLGMRQRLGVAQAILHRPKLLVLD
                     EPTNGLDPQGIRELRDYLRQLTRTEGITVFVSSHLLSEMELMCDRVAIIQSGRLVDIKQ
                     LKNTEGEVQTAEIAFEVNDAEQAYTLAGAGRVEGNQLLLHLEREQIAEMNSLLAANGVK
                     VYAIHPVARTLEEQFLEVTGGGSIG"
     gene            1155..1937
                     /locus_tag="NWS42_RS19225"
     CDS             1155..1937
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369846.1"
                     /locus_tag="NWS42_RS19225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease subunit"
                     /protein_id="WP_102998577.1"
                     /transl_table=11
                     /translation="MADFFKLVHNENLKIYLRVRTWIMLGLLAVITAVIPMLISLVSDQ
                     VSVWDGIALTTLFTFFLNTTFTVIVAADSVAGEFTWGTIKLLLIRPWTRSKILLSKYIS
                     VILFSLLGTIVLVLMVTLSSWLMLSRDAPAGSVPLFSDPISYVTLNFLYSYIALFVTMA
                     FAFMLSAVFRSSALAIGLSLFMMFTKTVYNSIGLFSTDRFEWTKYVLFTHMDLKKYLDM
                     PPGTVSSNLTFSLAVLAAYYVVFIAIAWVVFVKRDVST"
     misc_feature    2111..2127
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 22.86"
                     /tool="antismash"
     gene            2198..3283
                     /locus_tag="NWS42_RS19230"
     CDS             2198..3283
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426473.1"
                     /locus_tag="NWS42_RS19230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AI-2E family transporter"
                     /protein_id="WP_025364270.1"
                     /transl_table=11
                     /translation="MLQNNRFFRLTSGIVMLLLIIYLGTKVSFIFSPLVSLVSLLVVPL
                     MLSFFLYYLLRPIVDVMERRKIKRSISILLIYLVIGVLLFLFSWGVWPTLRDQVTNLFE
                     NAPKLIKSLVDQLSQWRHNQSLRQVLPPGEDPLSQLSDTLNEAFSTLSDYSVKLVSFVS
                     YFFIVLATFPILLYYMLKEGSKFGPKMLNFFPKKYHKDALEVMEDIDSALSSFIVSRVL
                     INVALGLMMFLGFLIIGLPYALLLAVISIFLNFIPYVGAVAASIPVVIIGFLESPSMAL
                     WSLVVVIVAQQIQDNWLSPIVFGKQLDIHPLTVVILLLVGGDLLGILGIILVIPLYMCG
                     KIVIRKVYHLFLERRVEDIME"
     gene            complement(3323..3787)
                     /locus_tag="NWS42_RS19235"
     CDS             complement(3323..3787)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369848.1"
                     /locus_tag="NWS42_RS19235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="WP_164964703.1"
                     /transl_table=11
                     /translation="MFKESKKKLSTPATTDTLLANSTSFEGTIEAEANIRIDGHFQGDI
                     YSTRTVVIGESAVVRSDIIARDVILAGKVFGSITTEGRLTITSTGELYGNTATPTLVIS
                     EGGVLNGTSQMNQTMDAESPAAIDKSEDENQSSFSQNIEDSQASLQPEAG"
     gene            complement(3833..4864)
                     /locus_tag="NWS42_RS19240"
     CDS             complement(3833..4864)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426474.1"
                     /locus_tag="NWS42_RS19240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="M23 family metallopeptidase"
                     /protein_id="WP_164964704.1"
                     /transl_table=11
                     /translation="MKKGSLNRRMTLLVIRDAQQPPKQLQCSAAAVILVPTLVIASIST
                     LVIGLQIRSSHIISQMETNLAVQSLQMEVTVADKDAAIGRLRQEVMELTNQATSIRERL
                     QRVTELEQQMQQFIRKHGNSSSASSNKSTITPLSWDASGHTGGELIAVHENTALLARQA
                     MDDFQEIEALLDTVERTVTRSIQQANAVQQNLERQQAAQLLQTRRLALAEQGRPSIWPV
                     GSRRMTSSFGYRSDPFTGKSAFHSGVDIAGQSGDPVYAAGAGTVLEAASSGARGKCIII
                     QHPDGLQSWYMHLSGMQVAPGDRVHKGQTIGLLGSTGRSTGPHLHFQIVKHNQPVDPLL
                     YVQ"
     gene            4987..6417
                     /gene="cls"
                     /locus_tag="NWS42_RS19245"
     CDS             4987..6417
                     /GO_function="GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cls"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686424.1"
                     /locus_tag="NWS42_RS19245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cardiolipin synthase"
                     /protein_id="WP_019686424.1"
                     /transl_table=11
                     /translation="MLWLLLVLIIFIFQTGTILLFEFRKPSKAVAWLFILFCFPLIGFV
                     VYYFVAQDYQKRKVVRKAGSQLFREFREHLWAQTKVIENVEQMHNPHFKHQERLFNQLI
                     RMSENPLTGCNRTRVLTNGEETFDAMLKAMEQAQHHIHVEFYIFRADEIGKKFQEVMIR
                     KAREGVKVRFIVDGVGSYHLPYSFIRTCNEAGVEFHYFLPPFFATLDRRINYRNHRKIV
                     VVDGTIGFVGGINVGDDYLGKYPKVGFWRDTHLQIEGDGVYFLQNVFLSDWKLASGERL
                     MDVNLFPPHTCTGDEEVQILSSGPDQVWDTIQELCFGALTVAKKRIWITTPYFIPDPGI
                     YEALKLAAVSGIDVKIIIPYQSDSKLVHLASLSYVQELLEAGVEFYQYRKGFIHAKVVI
                     VDDLLGSVGTANMDMRSFFYNFELTAVLFATSALERLSADFVEDISNSSKIDLNVFRRR
                     PRSQKIAEILTRMLSPLL"
     misc_feature    5007..5020
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 21.61"
                     /tool="antismash"
     gene            6612..7556
                     /locus_tag="NWS42_RS19250"
     CDS             6612..7556
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280287.1"
                     /locus_tag="NWS42_RS19250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YitT family protein"
                     /protein_id="WP_013369852.1"
                     /transl_table=11
                     /translation="MSVVSKEVQNLSEITDVTGELTKISKIPANPKNKVKRLLQRAAMI
                     VVGAALMAVGLEIFLVPNGVIDGGVTGISIMASKITGYPLGIFLTLLNLPFLLIGYKQI
                     GKTFALSTLFGIVVMSIGTALLHNVSALTPGEPLLGAIFGGVILGVGVGLVIRSGGSLD
                     GTEIVAILVSEKTPFSVGEIVLFVNIFILGSAGFVFGWPNALYSMIAYYIAMKMIDITI
                     EGLDQSKSVWIISDKYRDIGDALTDRLGRGVTYLEGEGGFTGESKKVIFVVITRLEEAK
                     LKSIVEDWDPHAFIAIGNIHDVKGGRFKKKGIH"
     gene            complement(7625..8527)
                     /gene="ligD"
                     /locus_tag="NWS42_RS19255"
     CDS             complement(7625..8527)
                     /EC_number="6.5.1.1"
                     /codon_start=1
                     /gene="ligD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369853.1"
                     /locus_tag="NWS42_RS19255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="WP_164964705.1"
                     /transl_table=11
                     /translation="MGRAVKGTITIEGHEIAITNPDKPLWPEAGVTKALYLRKLAVLAP
                     YLLKYCHQRLLTVIRWPHGIHGDFFYQKNAPHPRPDYIETALHDGIEYIVLGKLPQLLW
                     LGNQAALEFHPSLHRTGSQLPCEWMIDLDPSREVEPRIMEAASIVGEVLASLGLDSVPK
                     TSGATGVQIIVPIRTGITFDELRSIGLLVGQFVTEKHPHLFTLERLKKDRGTAIYFDYL
                     QHYQGKTLAAPYTPRARPGATVSTPLTWEEVRQDVSPLDYHLLNIEERLNQVGDLIDKV
                     PPQPVEDLLKHMRFKTHPT"
     misc_feature    complement(8676..8689)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 21.22"
                     /tool="antismash"
     misc_feature    complement(8676..8692)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 21.89"
                     /tool="antismash"
     gene            8740..10026
                     /locus_tag="NWS42_RS19260"
     CDS             8740..10026
                     /EC_number="2.8.2.-"
                     /GO_function="GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426477.1"
                     /locus_tag="NWS42_RS19260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sulfotransferase"
                     /protein_id="WP_164964706.1"
                     /transl_table=11
                     /translation="MIEKQGGKLVFLLSVPRSGSSLLTAILQNHSRLFATQEMWFLLSL
                     YDLPQSHARPYGGTGILRQFFKGMVPSDVLEQAARSYALEIYNGLLQGTTADMLIDKSP
                     RYYTVLEFVDRLFPAARRVWLIRNPLSIVASFKKVNQLRNGNFHLLKELGSPHFNMKMT
                     DITVGLFRYAHYFATPHPLAYPLMYEQLVANPRMEIEKLCHFLEIHYEPGMERYGNFAD
                     TPQSNLFYSMGAGDPFVMEHEQAHQNSVHSWQHLLSTMEIELYCRSIGARLFRQLGYGE
                     ALEQAEQMTGVHFEDDPDVELLSRRTHQFLQQSGFEWKTAYRMLEEQDADNKEFMTNAT
                     CHAAPHNPARMDMAQIVSDRRIQSLENRLAHSFEERNRLIAQLQSYQRPSQGFRSHLLA
                     RKFGRWASEVLSSIGKEKGGERECNCRNL"
     gene            10001..11926
                     /locus_tag="NWS42_RS19265"
     CDS             10001..11926
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369855.1"
                     /locus_tag="NWS42_RS19265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_263559983.1"
                     /sec_met_domain="GATase_7 (E-value: 1.2e-19, bitscore:
                     68.6, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 4.7e-25, bitscore:
                     87.2, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAIAGIYRFGHESVYAEEGEKMMQTLQKYPADRVCAWCEGSIFL
                     GCHAQHVTPESVHERLPFYDELRDLAITADAIIDNRSELFERLAVEQGRRIDITDSELI
                     LLAYDKWGVDAAGYLIGDFAFVIWDAKKHRLYGARDMTGNRTLYTYQHDGGFAFSTVVA
                     PLLVLSSLRKELHEPWLAEFLSIRAMQESVDIGTTAYKHINQLPPAHWFTMEEGKQSLC
                     KYACLDKVEPLRLKTGGEYIEAFREVFSQAVTARLRTHRAVGAALSGGLDSGAVASFAA
                     PTLHLQHKPLHAYSYVPVQDFTDWTPPTLLANERSYIQSTARYVGNIHENYMDFEGKSP
                     FSEIDIWLELMEAPYKYFENSFWIRGFYEKAQEHNVGVLLTGARGNFTVSWGPALDYYA
                     RQFRRLHWLQSWQGVRRYSKLTGSRMSHLLPVMLKKATSLDARTWWGRGKQSPIPSLIH
                     PEFAKRMNVEDIPVLSGTGWRKNADQTRKEKFSNLAIANKNGVMATKLSLRYGLWERDP
                     TGDSRVIRFCLSVPFEQYVQNGRDRALIRKAMRDHLPDDVRLNQRVRGVQPADWLHRML
                     PCWDTFMGELQVMCHDLRAAEYLNMDYIKTAMAKYRHPRPDQASDPNIRLFMHSLIVYR
                     FLCGLN"
     gene            11980..12108
                     /locus_tag="NWS42_RS19270"
     CDS             11980..12108
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369856.1"
                     /locus_tag="NWS42_RS19270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="paeninodin family lasso peptide"
                     /protein_id="WP_013369856.1"
                     /transl_table=11
                     /translation="MSKKEWQEPTIEVLDINQTMAGKGWKQIDWVSDHDADLYNPS"
     gene            12237..13292
                     /locus_tag="NWS42_RS19275"
     CDS             12237..13292
                     /EC_number="2.7.1.24"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821222.1"
                     /locus_tag="NWS42_RS19275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dephospho-CoA kinase"
                     /protein_id="WP_263559984.1"
                     /transl_table=11
                     /translation="MSPIAHDRSAHNSWVKYTAFGLRIASELTLPELIPAEPGLVEDVV
                     IRQVDLSAWRDQLEQANFVMQGERFMFLIPDTGIYAIRGGKEIEVSMLPGADPDTVRLF
                     VLGTCMGVLLLQKRVLPIHGSAVVIGGRVYAFVGESGTGKSTLAAAFRQAGYQMVSDDV
                     IAINVTASAAIVYPAYPQQKLGLESLLQLEALRENKYVRQRKHVLSLTDGNPVRAQYQD
                     LRRIAGELNKYAVPAVDEFYNDPLPLGGVFELVSDSPNRELMREGEPVVVTEQPLNKLE
                     CLHTLLQHTYRRAIIPRMGLGEWYFDTAARMARKVEGWRLLRDSSFFTASEVVQRVLEL
                     IHKEEKSYGSH"
     gene            13279..13581
                     /locus_tag="NWS42_RS19280"
     CDS             13279..13581
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369858.1"
                     /locus_tag="NWS42_RS19280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_016821221.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 2.3e-24,
                     bitscore: 83.5, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.9e-16, bitscore: 58.3,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAINPAKDHYRITYGEEVYVSDMDGEKVMMSIHTGKYYNLGFTG
                     GRIWELAESSPSLGDIVTVLTNEYEVDEEQCRQQVHTFVAELEREGLLRLLRELD"
     aSDomain        13327..13560
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_NWS42_RS19280_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="1.30E-26"
                     /identifier="RREFam002.1"
                     /label="NWS42_RS19280"
                     /locus_tag="NWS42_RS19280"
                     /protein_end="94"
                     /protein_start="16"
                     /score="83.5"
                     /tool="antismash"
                     /translation="EEVYVSDMDGEKVMMSIHTGKYYNLGFTGGRIWELAESSPSLGDI
                     VTVLTNEYEVDEEQCRQQVHTFVAELEREGLLR"
     gene            13583..14068
                     /locus_tag="NWS42_RS19285"
     CDS             13583..14068
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821220.1"
                     /locus_tag="NWS42_RS19285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_263559985.1"
                     /sec_met_domain="PF13471 (E-value: 8.7e-24, bitscore: 82.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIQRLLLLSPEVRRLLPEAWLELGCARFRQRRSFGDYSEAWGIC
                     MEETSWQCEIGQEKSIRHISQAVDVASRYTPWRSSCLVQAAAAMTLLVRRKLDCTLYLG
                     TAKNAAGQLAAHAWVRSGPIYVTGEREMGAFTVVGIFGRRAGTATNTRTADKGSSLF"
     gene            14112..15638
                     /locus_tag="NWS42_RS19290"
     CDS             14112..15638
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426482.1"
                     /locus_tag="NWS42_RS19290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_263559986.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 1.1e-84, bitscore:
                     282.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTKSILELANWNEDQEFTLLLSLLRKDVNLWQDTTTALQLQEMD
                     WSRLLRLTEHHRVVPTVYLQLKTLNHPFIPAELLRNLQAQYQRNTLRMLQLQAEAERLT
                     RLLTAHGIRVLMLKGPALAQQLYGDVSLRTSKDIDLLIAPDDMDEAEHCMKEAGYASKS
                     GEVRVLGSWKWKDHHASFLHPQKRVEIELHWRLHPDGGREPSFDELWDCRQMGKAAGVR
                     ASQMAPATSADNRNVMTSCMYTLGSEHQFLYLSAHGARHGWFRLRWLVDIDRLAVRAVD
                     WGALLPLMRRYGGLPAGGQAWALAAALLGTPIPEPMRPISATRQAHRLALSALAFMQAS
                     VPAPYPVANGAGTGRLARSELLPSVALTESMNTAPSDGGISANGTSGEAVLYAVSSAAG
                     ISKDASSILGDPVNTVNTAVSSLAKPVGAATVASSPAIAGATTTAFTPLPVSRAYLLSL
                     KEPRHRLWYLISRLFPSTRDSVVFPLPRQLHFLYIPLRPFLWLKRRFSKH"
     gene            15659..17518
                     /locus_tag="NWS42_RS19295"
     CDS             15659..17518
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821218.1"
                     /locus_tag="NWS42_RS19295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_263561026.1"
                     /transl_table=11
                     /translation="MTHLRLYMTKLYSVNGGALFLNIGCMMIVSLLEGVGIYMLVPMLA
                     AIGVFGGHSEMPFLSDGFDYFLPYIPKSWLLPGMLMFFVMLLTGQAWLQRYQSILNSRI
                     QQRFMRSLRIDVYTSFMKAQWAFYLRQRKSDFSHVMTTELARVSQGTNLLLQLTTAIMF
                     TLIQIALAFWLSPSLTGLVFLSGLALFVVFRRSIRNAKQLGDRTTELSQVYFAGITDHM
                     NGMKDIKSNLLEAANIHWFRSLTQRMEDNTTQFVRQNATTQFFHRIAAAVFVVSVLFVS
                     LQWLHVTPEKLIVLILIFSRLWPRFALIQASLEYIMSMISAFESVTKVQRECEAFSDPS
                     LIGLTSSKAHVPFDSSVYHLPSISARQPLSLRNGIECSHLDYCYEGAAEPALRHINLFI
                     PARGMTAIVGKSGAGKSTLVDVLMGFLRPSEGQIKIDGHVLDDELLPRWRQSFGYVSQD
                     PFLFHATVRENLKLADKDASEQQLWEALHFAAADNFVSRLPEGLDTVIGDRGIRLSGGE
                     RQRLVLARAILHHPPVLILDEATSALDGEHEAAIQAALERMKGQITLIVIAHRLSTIRH
                     ADQIVVLDQGEVIQQGNYHQLVEEERGAFSQLLSYQSTVSAAP"
     gene            complement(17538..18452)
                     /locus_tag="NWS42_RS19300"
     CDS             complement(17538..18452)
                     /GO_function="GO:0045027 - DNA end binding [Evidence IEA]"
                     /GO_process="GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309197.1"
                     /locus_tag="NWS42_RS19300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Ku protein"
                     /protein_id="WP_263559987.1"
                     /transl_table=11
                     /translation="MHTVWKGAISFGLVHVPVKMFSATEDKDISMRYIHKECGSPLSYV
                     RKCPVCDKEVEWEEIGKGYEYEKGKFVMFDKEELEQVSGQADKNIVILDFVDLQEIDPI
                     YFQKTYYLSPDQAGSNAYKLLMNAMKDTGKIGIAQISIRSKSSLAAIRVLDECLAVETM
                     FYPDEIRPIAQVPNLPGQEEVKEKELTMAKMLIEQLSTPFDAAKYTDQYRERLLDLIQH
                     KVAGEEVRIAPTQPQTNVVDLMAALQASIEAVKPVVTDPGPAAKKRPARKPAAVTAQQA
                     VAGDALTPTPVKKKRSPAKRKET"
     gene            18662..18901
                     /locus_tag="NWS42_RS19305"
     CDS             18662..18901
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369863.1"
                     /locus_tag="NWS42_RS19305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_013369863.1"
                     /transl_table=11
                     /translation="MPRKPYPQQISHRVGQSHIAFRGPAGGEQDALPPEFALSHGTPPP
                     FPVAVAHVDMEAVVSTSPSHSFPSLDVERIIDED"
     misc_feature    18918..18931
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.34"
                     /tool="antismash"
     gene            18952..19131
                     /locus_tag="NWS42_RS19310"
     CDS             18952..19131
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309199.1"
                     /locus_tag="NWS42_RS19310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="H-type small acid-soluble spore protein"
                     /protein_id="WP_013369864.1"
                     /transl_table=11
                     /translation="MNVERAKAIYDSSETIAVQLDGKPIWIEHVDEANGMATVQIGSRP
                     GDTQTVRVDRLEEQ"
     gene            complement(19264..19920)
                     /locus_tag="NWS42_RS19315"
     CDS             complement(19264..19920)
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013369865.1"
                     /locus_tag="NWS42_RS19315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="WP_013369865.1"
                     /transl_table=11
                     /translation="MNNRELIIRRIAQEFKDGEVVNLGIGLPTMAVDYIPENVNIMLQA
                     ENGIVGVGPKAAPGKERLDCIDSGGNYVTTVKGACFIDSALSFSIIRGGHVDVTVLGAL
                     EVDEKGNLANWMIPGKKVPGMGGAMDLVVGAKRVIVAMEHVNKNGAPKILKQCKLPLTA
                     VNVVKTIVTEKGVIDVIPGKGLMLKEIAPGLSVEDVQMATEAELMVSPHLKTIAV"
     gene            complement(19913..20581)
                     /locus_tag="NWS42_RS19320"
     CDS             complement(19913..20581)
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426488.1"
                     /locus_tag="NWS42_RS19320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CoA transferase subunit A"
                     /protein_id="WP_263559988.1"
                     /transl_table=11
                     /translation="MNKKVNSAEEAVKKIKDGDTVMVGGFMQGGIPEELVRALVDSNSA
                     DNLTIISNDTGTPELSIYRLVKSGRVKRIFASYIGSNPETGRLLMTKEAEVQLFPQGTL
                     AEKIRAGGAGLGGILTPVGVGTIVEEGKKKIKIDEREYLLELPLKADVALIRAHKADEA
                     GNLVINGSSRNFNLVMATAAEYVVAQTDEYVKAGEIDPNHINVPGIFVDAIVKVGGDNE
                     "
     gene            complement(20619..21359)
                     /locus_tag="NWS42_RS19325"
     CDS             complement(20619..21359)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686439.1"
                     /locus_tag="NWS42_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetoacetate decarboxylase"
                     /protein_id="WP_263559989.1"
                     /transl_table=11
                     /translation="MNQKDVLSLQSMPAASGSYGRPPCRFINREFFIITYESDPAAIRQ
                     AVPEPLQPDGSNTVSYEWIKMPDSSGLGSYEESGIVIPCTFQGEPCNFVAQMYLDNAPA
                     ILGGREIWGFPKKWAKPRLVVEDTETLTGTLHYNNVLVAMGTTPFKYNVLDENETAKAI
                     AKTQVNLKLIPDVDGTPKIAQLVAYNLENITVKGAWSGPARLSLIPHVNAPVADLPVKT
                     YVGGKHFIADLTLPYGRVLYDYLQ"
     gene            complement(21413..22597)
                     /locus_tag="NWS42_RS19330"
     CDS             complement(21413..22597)
                     /EC_number="2.3.1.9"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124: acetyl-CoA acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429859.1"
                     /locus_tag="NWS42_RS19330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="WP_167440842.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.9e-05, bitscore:
                     21.5, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKVAIVSPLRTAVGSFNKALAPLSAPELGATVMTACLQQSKLEP
                     SLIDQIFMGNVLQAGNGQNPARQAALKAGIPIDVPESTINTVCGSGLHAVALAYDSILA
                     EQGNIVLAGGMESMSNAPYLLKNARNGYKLGNGELVDSLVADGLTCPINHYHMGITAEN
                     VAEKYGISRLEQDAFAYESQIKAVEAKNNKVFEEQIVPVMIKNKKETTYFTEDEHIRGN
                     TTKEKLSQLKPAFKENGTVTAGNASGINDGAAAMLVVSEDKCKEHALKPLAYIKGYSLV
                     GVDPAYMGMGPVKAISSLLKKQGMSIDDIDLFEINEAFAAQALAVQKELGVSPEKVNVN
                     GGAIAIGHPVGASGARVLVTLVHEMVRRSSRYGVVSLCIGTGMGIAMLVENALI"
     misc_feature    complement(22589..22602)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.1"
                     /tool="antismash"
     misc_feature    22908..22922
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.83"
                     /tool="antismash"
     misc_feature    22915..22929
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.67"
                     /tool="antismash"
     gene            complement(23258..23788)
                     /locus_tag="NWS42_RS19335"
     CDS             complement(23258..23788)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027: isochorismatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429259.1"
                     /locus_tag="NWS42_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cysteine hydrolase family protein"
                     /protein_id="WP_263559990.1"
                     /transl_table=11
                     /translation="MGQTVLLLIDVQQAMFMYDEKLYREQEVVAHLQLLLTEARATGTP
                     VIFVQNTDEADEDFRENSVGWAIADVVRPLPHERIVRKSSWDSFYQTELRDVLQELGAE
                     QLIIAGMQTEFCLDTTCRSAYSLGYQNNVLVSDAHSTFDSKVLSGEQIVAHHNAVLDNR
                     FARLLTTADVHFS"
     misc_feature    complement(23811..23828)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.23"
                     /tool="antismash"
ORIGIN
        1 attacggtca ctcagcacta caagacccga ttcggtactt ttgatatcgt acagccttgt
       61 tatgtgacac aggaagatga ggagtggaga gtgctttgga tatacaagga gaaggaacag
      121 acgtgataag gtgctcccaa aggtgtttct gacaggataa atatggtaca atacttccaa
      181 aaccggaaat gtacagtaaa atggatgaca gcttcactcc gggtggaagg agtttcatag
      241 catgcagcaa gcgacagggc aggttcccgt catacggatg gagggtgtga gtaaaatcat
      301 ttcctcaaaa gcgatcgtgg acaatctgac actggaggtt ccggcaggac aggtatttgg
      361 tttcctcgga ccgaacggtg caggaaaaac gaccaccatt cgtatgatgg ttggcctgat
      421 ttcgatcagc aagggtgata ttcatatatg cggagacagc atcaataccg atttcgaaaa
      481 ggctgtttcc catgtcggtg cgattgttga gaacccggag atgtacaagt tcttgacagg
      541 ctatcaaaat ttgcagcatt ttgcgcgcat gtcgcctggg gtaacccaag aacgtataga
      601 tgaagcgatt cgtctggtgg gactcggaaa ccgaattcat gataaagtca aaacctactc
      661 gctcgggatg cgtcagcgac tcggggtagc tcaggcgatt ttgcatcgac cgaagctgct
      721 cgtgctggat gagccgacca atggcttgga cccgcagggt attcgtgaac tgcgggatta
      781 tttgcgtcaa ttgacccgta ccgaaggaat taccgtgttt gtatcaagcc atttgttgtc
      841 cgaaatggag ctgatgtgcg atcgcgtggc cattattcag agcgggcgat tggttgatat
      901 taagcagttg aagaatacag agggagaagt gcagacggcg gaaattgctt ttgaagtcaa
      961 tgatgctgag caagcttata cgcttgctgg agcagggcgc gtcgaaggca atcaactact
     1021 gcttcacctg gagcgggaac agattgcaga gatgaacagt ctgctggctg caaatggagt
     1081 caaagtgtat gcgattcacc cggttgcccg tacgttggaa gagcaatttt tggaagtgac
     1141 gggaggcggg tccattggct gatttcttca agctggtaca taatgaaaat cttaaaatct
     1201 acttgcgtgt gcgcacatgg atcatgctgg ggctgctggc ggtcatcacc gctgtgattc
     1261 cgatgctgat ttctctagtt tctgatcagg ttagtgtgtg ggatggcatc gcattgacaa
     1321 cgttgtttac atttttcctc aatacgacgt ttacagtcat tgtagctgct gattcggtgg
     1381 ctggcgaatt tacatgggga acgattaagt tgctgctcat tcgcccttgg actcgcagca
     1441 aaattctgtt gtccaagtat atatcggtga ttttgttcag cttgcttgga acaatcgttt
     1501 tggtcttgat ggttacgctg tcctcttggc tgatgttatc tagggacgca ccagcgggct
     1561 ctgttccgct gttcagtgac ccgatatctt acgtaacgct gaactttttg tatagctata
     1621 tcgccttatt cgtgaccatg gcttttgcat ttatgttatc cgcggtgttt cgttcgagcg
     1681 ccttggcgat tggtttgtcc ttgtttatga tgttcaccaa aacggtgtat aactctattg
     1741 gcttgttcag cacagatcgt tttgaatgga ccaaatacgt actctttacg catatggatc
     1801 tgaaaaaata tctagatatg ccgccgggta cagtaagttc caatctaacc ttttcgctcg
     1861 ccgtgctggc cgcttattat gtggttttta tcgcgattgc ctgggtggtt tttgtaaaac
     1921 gcgatgtctc cacctaaaca cgtatctctt agcatgtgtg attgaataac acgaggattt
     1981 tggcaaaatg ctgagatcac aaaaaggaag tccaatgcct agccatttgg acttcctttt
     2041 ttgctatgtt ggcactgttt tattcagtat ctatgtcatt tttgctacat attctgtaag
     2101 tgtgggtata ttgtaatcta ttttcagcaa gaatcaatca atagggtcgg tttgcgttta
     2161 aagaagtaga gagaaacaga acaggggggc gcatgatatg ctgcaaaata atcgtttttt
     2221 tcgtctgact tcaggaatcg tcatgctatt gctcatcatt tacttgggca ccaaagtaag
     2281 ctttatattc agtcctcttg tatccttggt cagcttgctg gttgtaccgt tgatgctatc
     2341 ttttttcctc tattatttat tgagaccgat tgtagatgtc atggaacgga gaaaaatcaa
     2401 acgatcgatt tccatattgc tgatttattt ggttattggc gtgttactgt tcctcttctc
     2461 atggggagtg tggcctacgc tccgtgatca ggtaaccaat ttatttgaga atgcgccgaa
     2521 gcttattaaa agtctggttg atcaattgag ccaatggcgg cataatcaat ccttgagaca
     2581 agtactgcct ccgggtgaag acccgctgtc acaactgtca gatacgttaa acgaagcttt
     2641 ttcaacgctg tcagattact cggtaaagct ggtatcgttc gtatcctact tttttatcgt
     2701 attggctacc tttcccatat tgctctatta catgttgaag gaagggagca agtttggtcc
     2761 caaaatgctt aacttctttc ctaaaaagta tcataaggac gcgctggaag ttatggagga
     2821 tattgatagt gcgctcagca gctttatcgt cagtcgagtg ctgatcaatg tcgcgttggg
     2881 cttgatgatg ttccttggat ttttgattat tggtttgcct tatgccttgc tgcttgccgt
     2941 tatttcgatt ttccttaatt ttattcccta tgtgggggca gtagctgctt ctatacctgt
     3001 ggttatcatt ggatttctcg aatcgccttc catggcgctc tggtcgctgg tggtggtgat
     3061 cgttgcacag caaattcagg ataattggct gtcgccgatt gtctttggca agcagttgga
     3121 cattcatccg ttgactgtcg tcattttatt gctagttggt ggcgacctgc tcgggatctt
     3181 gggtatcatt ttggttattc cattatatat gtgcggcaaa atcgtcatac gcaaggtgta
     3241 ccatttattt ttggaacgca gagtggagga tattatggag tgattggagg atgtactgga
     3301 tatataggag tataagccgg ccctatcccg cttcaggttg tagggaggct tggctgtcct
     3361 ctatgttttg gctgaaactg gattgatttt catcctctga tttatctata gcagctggcg
     3421 actctgcatc catagtttgg ttcatttgac ttgtgccgtt cagtacgccg ccttcagaaa
     3481 tgaccaatgt aggggtcgcc gtatttccgt acagttcgcc tgtcgatgta atggtcagcc
     3541 gtccttctgt ggtgatgctg ccgaatacct tgccggcaag aatgacgtcc cgtgcaataa
     3601 tgtccgaacg tacaactgct gattctccaa tgacgacagt gcgagtgctg tagatatcgc
     3661 cctgaaaatg accgtcaatg cgaatgttgg cctctgcctc aatcgtacct tcaaagctgg
     3721 tgctgttcgc gagcagtgta tccgtcgtcg ccggggtgga gagctttttt ttggattcct
     3781 tgaacatgat cgttttcctc caatccttta ttgtcggtat tttgcaaaac ccttattgta
     3841 catacagcaa tggatcaaca ggttggttat gcttgacgat ctgaaaatga agatgaggtc
     3901 ctgtgcttct cccggtactt cccagcaggc caatggtctg tcctttgtga acacggtccc
     3961 ccggagccac ctgcataccg cttaggtgca tataccagct ttggagtccg tcaggatgct
     4021 gaatgatgat gcacttgccg cgagctccgc tggaagcggc ctccagcaca gtgcctgccc
     4081 ctgctgcata gacaggatca ccggattgac cggcaatgtc cacgcctgag tgaaaagcgg
     4141 atttccctgt aaatgggtcg ctgcgatagc cgaaactgga ggtcatgcgc cgcgaaccaa
     4201 caggccaaat ggagggcctg ccttgttcag caagtgccag tcttcgggtc tggagcagct
     4261 gcgcggcttg ttggcgctcc aggttctgct gtacggcgtt tgcctgttga atagagcgcg
     4321 taaccgtgcg ctccactgtg tccagcagag cttcgatttc ctgaaaatca tccatggcct
     4381 ggcgggctag aagagctgta ttttcatgca ctgcgatcaa ttcaccgccg gtatggccgg
     4441 aagcatccca ggagaggggg gtgatggttg atttattgct gcttgcgctg gaggaatttc
     4501 catgtttacg aatgaattgc tgcatttgct gttccagctc tgttacacgc tggagacgtt
     4561 cgcgaatact ggtggcttga ttcgtcagct ccatgacttc ctgccgcagt cgcccaatgg
     4621 ctgcgtcttt gtctgctacc gtgacctcca tctgcaaact ctggacagca agattggttt
     4681 ccatttggga aataatatgg gaagaacgga tttgcagccc gatcacgagc gtagagattg
     4741 atgcgatcac aagggtcggg acgagaatga cagcggctgc ggagcattgt agctgcttgg
     4801 gcggttgctg tgcatcccga atgacaagca gggtcatccg gcgatttagg cttccttttt
     4861 tcataatcgc tcctttccgg acatcgctcc tatagtgctt ggttactatc tattcagctt
     4921 aagaggcaaa catgtcaaaa aaattggagc tttgtataaa acgtagatat ataaggaggc
     4981 atagacatgc tgtggttgct gttggtttta attattttta tttttcagac cgggacgatt
     5041 ctgctctttg aatttcgcaa gccgtccaaa gcggtagcct ggctctttat tttgttttgc
     5101 ttcccgctta ttggttttgt ggtgtactat tttgttgctc aggattacca gaagcgtaaa
     5161 gtggttcgta aagcaggatc gcagctattt cgtgaattcc gtgaacatct gtgggcgcaa
     5221 accaaggtga ttgaaaatgt agagcaaatg cacaatcccc atttcaagca tcaggagcgt
     5281 ctgtttaacc agttgattcg tatgtcggaa aacccgttga ctggctgcaa ccgtacacgt
     5341 gtgctgacga acggggaaga gactttcgac gccatgttga aagcgatgga acaggcacaa
     5401 catcatatcc atgtggagtt ttacatattt cgggcagacg aaatcgggaa aaaatttcag
     5461 gaggtcatga tccgtaaggc acgcgaggga gttaaggtac gattcattgt ggacggcgta
     5521 gggagctacc atttacccta ttcatttatt cgcacctgta atgaggctgg ggtcgaattt
     5581 cattattttc taccgccgtt tttcgccacg ctggaccgcc ggattaacta tcgcaatcac
     5641 cggaaaatcg tggtggtgga tggcacgatc ggatttgtgg ggggaatcaa tgtaggcgac
     5701 gattatctcg gaaagtaccc caaagtggga ttttggaggg atacgcattt gcagattgaa
     5761 ggagatggcg tttattttct gcaaaatgtg tttcttagcg attggaagct ggcttcggga
     5821 gagaggctaa tggacgtgaa tttatttcct ccacatacct gcacagggga tgaggaagta
     5881 cagattttga gcagtgggcc cgatcaggta tgggatacga ttcaggaatt gtgttttgga
     5941 gcgcttacgg ttgccaaaaa gcggatttgg ataacgacgc cgtattttat tcccgatccg
     6001 ggaatttatg aggcactgaa gctggcggcc gtcagcggca tagatgtgaa aattattatt
     6061 ccctaccaat cagattcgaa actggtccac ctggcgtcac tgtcctatgt gcaagaactg
     6121 ctggaggcag gagtggagtt ctaccaatat cgaaaaggat ttattcacgc taaagtggtg
     6181 attgtggacg atttgctcgg ctcggttggg accgccaata tggacatgcg tagttttttc
     6241 tataattttg agttgaccgc tgtacttttt gcaacttcgg cgttggaacg gctttcggca
     6301 gattttgtag aggacatttc caactcttcc aaaatagatt tgaacgtgtt ccgcagacgc
     6361 cctcgatctc aaaaaatagc tgaaattcta actcgcatgc tctctcccct cctttagagg
     6421 cggttttcgc cgtttttatc aatataatcc cgctttattg tgatttatgt gctctttgct
     6481 aacttttttg cgcaaaatca cagttttatg atatactaat cacataacta agcgccgtaa
     6541 aggagagggg actgcggaga gcagcccctt tttcggtcca atataccata ctgtaacggg
     6601 gggatttata tatgagtgta gtgagcaagg aagtccaaaa tctgtctgag atcacagatg
     6661 taaccggaga gttgaccaag atcagcaaaa ttccagctaa tccaaagaac aaagtaaaac
     6721 gcttattgca gcgtgcagcc atgatcgtgg ttggtgcagc gctgatggcc gtgggattgg
     6781 aaatattcct ggttcctaac ggcgttattg acggcggcgt tacaggtatt tccattatgg
     6841 cctccaagat tactggttat ccgctcggta ttttcctgac cctgttgaac cttccatttt
     6901 tacttatcgg ctataaacaa atcggtaaaa ccttcgcttt atccacatta ttcggcatcg
     6961 tcgttatgtc catcggaact gcgttacttc acaatgtaag tgcattgacg cctggggaac
     7021 cgctgcttgg cgctatcttc gggggtgtca ttctcggcgt gggagtgggt cttgtgattc
     7081 ggtccggcgg ctcgctggat gggacagaga tcgtggctat tctcgtcagc gaaaaaactc
     7141 ctttttcggt cggtgagatc gtattgttcg ttaatatttt cattctggga agtgcgggtt
     7201 ttgtattcgg ttggccgaat gccctgtatt ccatgattgc ttattatatt gctatgaaaa
     7261 tgattgatat tacaattgag ggtctggatc agtccaaatc ggtctggatt attagtgata
     7321 aataccgtga tatcggagat gctctgacgg atcgtcttgg acggggtgta acttacttgg
     7381 aaggtgaagg tggctttaca ggcgagagca aaaaagtgat tttcgtcgtc attacccgac
     7441 tggaggaagc gaaactcaag agcatcgttg aggattggga cccgcacgcc ttcattgcaa
     7501 ttggtaatat tcacgatgtg aagggcggac gttttaagaa aaaaggaata cactagcgca
     7561 aggggacaat ttctcagcct atggctgcgg ggttcgtccc cttttttgtg cttcaaagca
     7621 tggatcaggt tgggtgtgtt ttgaagcgca tatgtttgag gaggtcctct acgggctggg
     7681 gaggcacctt gtcgatcaag tcgcctacct ggtttaaccg ttcctcaata tttagcaaat
     7741 ggtaatcaag tggtgacaca tcttgccgta cctcttccca ggttagcgga gtcgagaccg
     7801 tagcaccggg acgtgcccga ggagtataag gagcggctag ggtttttccc tgataatgct
     7861 gcaagtaatc aaaatagatg gctgttccac gatccttctt taaccgttcc agtgtgaaca
     7921 gatgcgggtg cttttccgtc acaaattgtc caaccaacag tccgatgctg cgtagctcgt
     7981 caaaggtgat gcctgtacgg atcgggacga taatttgtac acctgtggcc ccggaggttt
     8041 tagggacaga atccaaccca agcgaagcca atacttcgcc tacaatggag gcggcctcca
     8101 taatacgtgg ctcgacttct ctggatggat ccagatcaat catccattca cagggcagtt
     8161 ggcttcctgt tctgtgcaga gagggatgaa attccaacgc agcttgatta ccgagccaga
     8221 gtagttgggg cagcttgcca agcacgatat actcaatgcc atcatgcaga gcggtttcaa
     8281 tatagtctgg ccgaggatgt ggggcatttt tctgataaaa aaaatctcca tggataccat
     8341 gtggccaacg gatgacagtc aatagacgct gatgacaata tttgagcagg tagggggcta
     8401 ataccgcaag cttgcgaaga tatagcgcct tagttacacc tgcttcgggc cacagcggtt
     8461 tatccgggtt tgtgattgca atttcatgac cttcaatcgt aatcgtacct ttaacagctc
     8521 gtcccatagt gctatgctcc tttggttaac atgcagcaag atcattgttt acagacaact
     8581 ggcagcattt ctacatctac ataaggttta acatgagcgt gcagcacaga atttatccat
     8641 caaagggatt gacaagatac attatgtatc ataaattata ataaaaatac gatatgtatc
     8701 tattaaaacg tcttgagcag aggtagagga ggagtgtgct tgattgaaaa gcagggcgga
     8761 aagctagtat ttctgttgag tgtccctcga agtggaagct cgctactaac tgccattttg
     8821 caaaaccatt cccgtctgtt tgccacgcag gaaatgtggt ttctgctcag tttatatgat
     8881 cttcctcagt cacatgcacg gccatatgga ggaacaggaa tactccggca attttttaaa
     8941 ggtatggtcc cttcagatgt attggagcag gccgctcgtt cgtatgcact tgaaatatat
     9001 aatggcctgc ttcaagggac gaccgctgat atgcttatcg acaaatcacc gcgttactat
     9061 acagtgcttg aatttgtaga ccgtttgttc ccggctgccc gtcgagtgtg gcttatccgc
     9121 aatcctttgt ccatcgtggc ctcgtttaaa aaggtcaatc agctccgaaa tgggaacttt
     9181 cacctgctga aggaactggg aagtccgcat tttaatatga aaatgacaga tattacggtt
     9241 ggtttgttcc ggtacgctca ttattttgcc acacctcatc cacttgcata cccgctgatg
     9301 tatgaacagc tcgtagccaa tccaaggatg gagattgaga agctgtgtca ttttctagag
     9361 atccattatg agccggggat ggaaaggtac gggaacttcg cggatacgcc ccaatccaat
     9421 cttttttata gcatgggagc gggtgatccg tttgttatgg agcatgaaca ggcgcatcag
     9481 aattcggtac atagttggca acatctgttg agtacaatgg aaattgaact gtattgcaga
     9541 agtattggag ctcgcttgtt taggcagctt ggttacggag aagcgctgga acaggctgag
     9601 cagatgactg gagtccactt tgaggatgac ccggatgtag agctgttgag tcggcgtaca
     9661 caccagttcc tccaacaaag tggctttgaa tggaagacag cttatcggat gctggaagaa
     9721 caggatgcag ataacaaaga attcatgaca aatgcaacct gtcatgctgc acctcacaac
     9781 ccggcaagga tggatatggc acagatcgtg agtgatcgac gaattcaatc actggaaaac
     9841 agactagcac atagctttga ggaacggaac cggctgatcg cgcagctcca aagctatcag
     9901 cgcccaagcc aaggattccg gtcccattta ttggcccgta agttcgggcg ctgggcgtct
     9961 gaagtattat caagtatcgg aaaagaaaag gggggagaac gtgagtgcaa ttgccggaat
    10021 ctatagattt gggcatgagt ctgtatatgc tgaagaaggc gaaaaaatga tgcaaactct
    10081 gcaaaaatat cccgctgacc gcgtgtgcgc ttggtgtgaa ggttccattt ttttgggatg
    10141 tcatgcccag catgtgaccc cagaatctgt tcatgagcgt cttccctttt atgatgagct
    10201 aagagatttg gccatcacgg ccgatgcgat cattgacaac cgttctgagc tgtttgaacg
    10261 actagcagtt gagcaggggc ggcggataga tatcacggac agcgagctga ttttactggc
    10321 atacgacaaa tggggtgttg atgcagcagg ttatctgata ggtgattttg cctttgtcat
    10381 ttgggatgct aagaaacatc ggttatatgg tgctagagat atgacaggca atcgtacgct
    10441 gtatacctac caacatgacg gaggatttgc gtttagcacg gtagttgctc cgctattggt
    10501 tctgtcctct ctgcgaaaag aactgcatga accatggctg gccgaattct tatctatccg
    10561 tgcaatgcag gaatccgtcg atatcgggac aaccgcctac aagcatatca accagctacc
    10621 tcctgcacat tggttcacta tggaagaggg aaagcaatcg ctctgtaaat atgcatgtct
    10681 ggataaggta gagccgttgc ggctcaaaac aggcggcgaa tacatagaag ctttccgcga
    10741 ggtattctcc caggcggtaa ctgcccgatt acggacgcat cgcgctgtag gggctgccct
    10801 gagtggggga ttggattccg gagcggttgc cagctttgcc gcgccaacgc tgcacttaca
    10861 acacaagccg ctacatgcct acagctatgt acctgttcaa gattttacag attggacacc
    10921 acctacattg ctggcaaatg aacgcagcta tattcagtcc acggctcgct atgtgggtaa
    10981 tattcatgaa aattatatgg attttgaggg gaaaagcccg ttttctgaaa ttgatatttg
    11041 gctggagttg atggaggctc cctacaaata ttttgaaaac tccttctgga ttagaggttt
    11101 ttatgagaaa gcacaggaac ataatgtcgg agttctgttg actggggcac gcggcaactt
    11161 taccgtttcg tgggggcctg cactggacta ttatgcccgt cagtttcgcc gtttgcattg
    11221 gctgcaatct tggcagggag tgaggcggta tagcaagctt acaggcagtc gtatgtctca
    11281 tttactgccc gtgatgttga aaaaagccac ctcacttgac gctcgcacat ggtggggacg
    11341 tggcaagcaa agtccgattc cttcactgat tcacccggag tttgccaagc ggatgaatgt
    11401 agaagatatt cccgtattga gtggaactgg ctggaggaaa aatgccgatc agacaagaaa
    11461 agagaagttt tcaaatttgg cgattgccaa caaaaatggt gtcatggcga ccaaactttc
    11521 actccgttat ggcctgtggg aacgtgatcc gaccggggac agccgtgtca ttcgcttttg
    11581 cttatcggta ccttttgaac aatatgtaca aaatggccgg gaccgtgccc tcattcgtaa
    11641 agctatgcgt gatcatctcc ctgatgatgt tcggttgaac cagcgagtac gcggcgtaca
    11701 gcctgcggat tggctacatc gtatgcttcc ttgctgggac acgtttatgg gagagctgca
    11761 agtgatgtgt catgatttac gagctgccga atatctcaat atggactaca ttaagacggc
    11821 tatggcgaaa taccgacatc cccgtccgga ccaggcatcc gatccgaata tacgactatt
    11881 catgcacagc ctgatcgtgt accgttttct ctgtggtttg aactgatttg aatctaaccg
    11941 atcagacggc gtgccattcg tgggagggga ggtgatgaga tgagtaaaaa agaatggcag
    12001 gaaccgacga ttgaggtgct ggatattaac cagactatgg cgggtaaagg ctggaaacag
    12061 attgattggg tgtcagacca tgatgccgat ctgtataatc cgagctaatg gaagataatt
    12121 atacatgagc cgatgttggg ctgagatcca gatagcttac tgatcacgag ccgtctttat
    12181 ttcattcggg ttaggttgga ccggaagaaa taaagacgga attttaagaa cagggagtga
    12241 gtcctatcgc gcatgacagg agtgcccata acagttgggt gaaatataca gcctttggac
    12301 tcaggattgc cagtgagctg actctgcctg agctgatccc agcagaaccg ggattggtag
    12361 aggatgtggt catccggcag gttgatttgt cagcatggag ggaccaactg gagcaggcca
    12421 attttgtaat gcagggtgaa cgctttatgt tcctaatacc ggacacgggc atttatgcca
    12481 tcaggggagg caaggaaatc gaggtaagta tgttgcctgg ggctgatcct gacaccgtgc
    12541 ggttgtttgt actgggcaca tgcatgggtg ttctcctgtt gcaaaaacgg gttttaccta
    12601 ttcatggaag tgcggtcgtc atcggaggtc gggtctatgc atttgtaggt gaatccggca
    12661 cgggaaaatc gacgctggct gctgccttta gacaggctgg ctatcagatg gtcagtgatg
    12721 atgtgattgc tataaacgtg acagcttccg cagccatcgt atatccggca tatccgcagc
    12781 agaagctggg actggaaagc ttgcttcaat tggaggcatt acgggagaac aagtatgtac
    12841 ggcaaagaaa gcatgttctt tccctaactg acggtaatcc tgtaagggca caataccaag
    12901 atttgcgtag gattgcaggt gagctcaata aatatgccgt gcctgctgtg gacgaatttt
    12961 ataacgatcc gcttcccctg ggcggtgtat tcgagcttgt gagcgattct cccaaccgtg
    13021 agttgatgcg tgaaggggag ccagtggtgg ttacggaaca gcccttgaac aaactggagt
    13081 gcttgcatac attactacag catacatatc gtagggcgat cattccgcga atgggtttgg
    13141 gtgaatggta ttttgataca gcggcccgga tggctcggaa agtggaaggc tggcggctgc
    13201 tgagggatag ctccttcttt acggcgtccg aggttgtaca acgcgtactt gaactcattc
    13261 ataaggagga aaagagttat ggcagccatt aatccagcta aggatcatta tcgcatcacg
    13321 tatggtgaag aagtctatgt aagtgatatg gacggagaaa aagtgatgat gagtattcat
    13381 actggaaagt attataattt aggcttcaca ggaggacgga tctgggaact ggctgaatcc
    13441 agtccttcac ttggagatat tgtaactgtc ctgacgaatg aatatgaagt ggacgaggaa
    13501 cagtgcagac agcaggtgca tacctttgta gctgaattgg agcgggaagg attgctgagg
    13561 ctcctgcggg agttggacta acatgcggat acaacggctt cttctcttgt ctccagaggt
    13621 caggagattg ttgccggaag cctggctgga gctgggatgc gcgagatttc gccagcgtcg
    13681 ttcttttgga gattactcag aggcttgggg aatatgtatg gaagaaactt cttggcagtg
    13741 cgagatcggg caggagaagt ctatccgtca catttcacaa gctgtggatg tagccagccg
    13801 ttatactcct tggcgcagta gctgtcttgt acaagccgct gccgctatga cgttgctggt
    13861 acgcagaaaa ctggactgta cactgtatct gggcacagcc aaaaatgctg cgggacaatt
    13921 ggcagcacat gcatgggtac ggagtgggcc gatctatgtt acgggagaac gagagatggg
    13981 agcgtttacc gtggtgggaa tattcgggag acgtgctggg acagctacaa atacgaggac
    14041 agcggataaa ggcagttctc tgttttagtg tgagaaaaaa gagtgttcaa agaatcgaag
    14101 gagaagaatc catgactacc aagtctattt tggaacttgc gaattggaat gaggatcaag
    14161 aattcacgtt attactctct ctacttcgta aagacgtcaa tctatggcag gacacgacga
    14221 ctgctctcca gctgcaggag atggattggt cccgcttgct gcgtctgaca gaacaccatc
    14281 gtgtggtacc tacagtgtat ctccagctga aaacgttgaa tcacccgttc atccccgcag
    14341 aattgctgcg aaaccttcag gcacaatatc aacgaaacac gctacgcatg ctccagcttc
    14401 aagcagaagc cgagcgtctg actaggctgc tcacagctca tggaatccgt gtgctgatgc
    14461 tgaagggtcc tgctctggcg cagcaattat acggggatgt ttccctgcgt acctctaaag
    14521 acattgattt acttattgcc ccggatgaca tggatgaagc ggagcattgt atgaaggaag
    14581 ccggatatgc gtccaaaagc ggagaagtgc gcgtccttgg tagctggaag tggaaggatc
    14641 atcacgcctc cttcctacat ccgcaaaaga gggtagaaat cgagttgcac tggcggcttc
    14701 acccggatgg agggcgggag ccctcctttg acgagctttg ggactgtcgg caaatgggga
    14761 aggccgcagg agttcgagcc agtcagatgg caccggcgac ttctgccgac aacaggaatg
    14821 tgatgacatc ctgtatgtat acgcttggca gcgagcatca atttctatac ttgagcgcac
    14881 acggagcgcg tcacggctgg ttcaggctgc gctggttagt agacatcgac aggcttgcgg
    14941 tacgcgctgt cgattggggg gcgctactgc cgctaatgcg ccgttacggg ggcctgcctg
    15001 cgggcgggca ggcttgggca ctggccgctg cgttgttggg tacgccgatc ccggagccga
    15061 tgcgtccgat atcggctacc cggcaggcgc acaggcttgc gctgagcgcg ctggccttta
    15121 tgcaggccag cgtccctgcg ccgtacccgg ttgccaatgg ggcgggtaca ggcagactcg
    15181 ctcggtcaga gctattgccc agcgtagctt tgaccgagtc aatgaatacc gccccttcgg
    15241 atggcggtat ttcagctaac ggaacctcgg gcgaagctgt tctgtacgcc gtcagtagtg
    15301 ctgctggcat atcgaaagat gccagcagca tccttggtga cccggtgaat acagtgaaca
    15361 ctgctgtaag tagccttgcc aagcctgtag gtgctgctac agtcgcctcc agtccagcca
    15421 ttgctggggc tacaacaaca gcgtttactc ctctgccggt tagccgcgct tacttgcttt
    15481 ccttaaagga gccaagacac agactatggt atctcatcag ccgtttgttt ccttctacga
    15541 gggattcagt cgtttttcca ctgcctcgcc agctgcattt tttgtatatt cctttacgtc
    15601 catttctttg gctgaaacgg cgattttcaa agcactaacc cgataaggag tgtacatgat
    15661 gactcacctg cgattatata tgaccaagct gtatagcgtg aacggcgggg cgttatttct
    15721 taatattgga tgtatgatga tcgtcagcct actggagggt gtcggcattt atatgcttgt
    15781 tcctatgctg gcggccatcg gcgtatttgg cggtcattca gagatgccat ttctgtcgga
    15841 tggatttgat tattttttgc cgtacattcc taaaagctgg ctgttgccgg ggatgctaat
    15901 gtttttcgtc atgctgctta ccgggcaggc ttggcttcag cgttaccagt ccattcttaa
    15961 ctcacggatt cagcagcgtt ttatgcgttc ccttcgcata gacgtataca cgtcctttat
    16021 gaaagcgcag tgggcttttt atttgcgtca gcgtaaatct gattttagcc acgtgatgac
    16081 gaccgagttg gcgcgtgtca gtcagggaac gaatttgctc cttcaactga ccacagccat
    16141 catgtttacg ctaatccaga ttgcgctggc tttttggttg tccccttctt taaccgggct
    16201 ggtgtttttg agtggcttgg cgctgtttgt cgtgttccgt cgctccatcc gaaatgccaa
    16261 acaattaggt gatcgtacca ctgaactgtc acaggtttat tttgccggaa ttacggatca
    16321 tatgaacggg atgaaagata ttaaaagcaa tctgctggaa gcggcgaata tccattggtt
    16381 tcgctctctg acacagcgta tggaggataa tacgactcag tttgttcggc aaaatgcgac
    16441 cacgcagttt tttcatcgca tagcggcagc tgtttttgtc gttagtgtcc tatttgtgtc
    16501 gctccagtgg ctgcatgtta cacctgaaaa actgattgtg ctcatcctta tattttcccg
    16561 cttatggccg aggtttgccc ttattcaagc gagcctggaa tacatcatgt ctatgatctc
    16621 tgcttttgag agtgtaacca aagtgcaacg agaatgcgag gctttctcag acccctcgtt
    16681 gatcgggctt acatcttcta aggctcatgt cccattcgat tcatcggtat atcatctacc
    16741 atctatttct gcacggcaac ccctatccct tcgtaatgga atagaatgca gccatttgga
    16801 ttattgctac gagggtgcgg cagaacctgc actacgtcat attaatttgt tcatacccgc
    16861 ccggggaatg accgccattg taggcaaatc aggagcaggc aaaagcacct tggttgatgt
    16921 cctgatgggt tttttacgcc cttctgaggg acagattaaa atcgatggtc atgtgctgga
    16981 tgacgaattg ctgccgcggt ggcggcagtc gtttggttac gtgtcacagg acccatttct
    17041 gttccatgcg accgtccgcg aaaatttgaa gctggctgat aaggatgcca gtgaacagca
    17101 gctatgggag gcgctacatt ttgcagccgc agacaacttt gtatccagac tgcctgaagg
    17161 gctggacacg gtcattggtg atcgcgggat ccgtctttcc gggggagagc gtcaacggct
    17221 ggtattagcg cgggccattt tgcatcatcc tcctgtactc attttggatg aagcgacaag
    17281 tgcgctggat ggtgagcatg aggctgcaat tcaggcggca ttggaacgga tgaaaggtca
    17341 aataacgttg atcgtgatcg ctcatcgttt gtcaacgatt agacatgcag atcaaattgt
    17401 tgttctggat caaggcgagg tcatacagca ggggaactac caccaactgg tagaagagga
    17461 gcggggggcg ttcagtcagc tactttcgta tcaaagcact gtcagcgccg ccccttagtc
    17521 agactttcct gtaaagctca cgtttcttta cgtttagcag ggctcctttt ctttttgact
    17581 ggagtcggag tgagggcgtc gcctgccaca gcctgctgtg ctgtcacggc tgctggttta
    17641 cgcgcaggac gttttttcgc agcaggacca ggatcggtaa cgacaggctt cactgcctca
    17701 atactggctt ggagggcagc cattaaatca accacattag tctgaggctg tgttggggca
    17761 attcgcacct cttcgccagc tactttatgc tggataagat ccagcagacg ctcgcggtac
    17821 tgatccgtat actttgctgc atcaaaaggg gtagatagct gctcaatcaa catttttgcc
    17881 atggtcagct ctttctcttt aacctcctcc tgtccaggta agttgggaac ttgagcaatg
    17941 ggacggattt cgtccggata aaacatggtc tccaccgcca agcattcatc cagcacgcga
    18001 atggctgcta gactactttt tgagcggatg gagatttggg caattccgat tttcccggta
    18061 tccttcattg cattcatgag tagcttatac gcattcgagc cagcctgatc cggggacaaa
    18121 taatacgttt tctgaaagta gatcggatca atttcctgta aatccacaaa gtccaaaatt
    18181 acgatatttt tatcggcttg tccgctaact tgctccagtt cttccttgtc gaacatgaca
    18241 aatttgcctt tttcatattc gtaccctttt ccaatttcct cccattcaac ctccttatca
    18301 caaacagggc atttccgtac gtagcttaat gggctcccac actccttgtg aatataacgc
    18361 atggaaatgt ccttgtcctc agttgctgag aacattttaa ctggcacatg aaccagcccg
    18421 aagctgatcg cgcctttcca gaccgtatgc ataccgccag tcctcctttt tttaatccgc
    18481 atatttcata tttcgattcc gggcacccgg gctgtgagca tttcattttt cacttagtat
    18541 gcttcgtaca aaagattgta tgagatgcaa atgaaggctc gaattgcttt cacttgcgca
    18601 tggtttcggt ttttttggtg aatgttaagc catatccgat tcaaaaggag gtatacaact
    18661 gatgcctaga aaaccctatc ctcagcagat cagtcatcgt gtggggcaaa gtcacattgc
    18721 ctttcgaggg cctgctggag gcgaacagga tgcattgccg cccgagtttg ctctttcaca
    18781 tgggacgccg ccgccttttc ccgtagctgt tgctcatgtg gatatggagg ctgtagtttc
    18841 aacatcgcct tctcatagct ttccttcatt ggatgtggaa cggattatag acgaagacta
    18901 ggtgacctat attttgaatt ttgcacaaaa aaagagtagg aggagaacag catgaacgtg
    18961 gaacgagcaa aagccattta tgattcatcc gaaacgattg ccgtccaatt ggatggcaaa
    19021 ccgatttgga ttgagcatgt agatgaagcg aatggtatgg caacggtaca gatcggcagc
    19081 agacctggtg atacacaaac cgttcgtgta gatcgactgg aagaacaata gaagagaggc
    19141 tccaacctaa aaaggctcgc aattgctcaa tgagcaatta gcgaacctta ttttatataa
    19201 tgaatgcgat tgtgaagaca agttaaacgc cagacaagtt aatttgaagg tggaactgat
    19261 tttttacact gcaatggtct tcaaatgtgg agaaaccatc aactcagcct ctgtcgccat
    19321 ttgaacatct tccaccgaca aacctggagc aatttccttg agcatcagcc ctttacccgg
    19381 aataacatca atgacacctt tttcggttac aatggtcttt accacattta cagcggttag
    19441 gggtagcttg cattgtttca gtattttcgg cgccccattt ttgttcacat gctccatggc
    19501 tacaatcact ctcttggctc caaccaccaa atccatggcc ccacccatgc cgggcacctt
    19561 tttcccgggg atcatccagt ttgcaaggtt gcctttttca tcgacctcca gggcacccaa
    19621 aacggtgacg tccacatgcc ctccacgtat gatggaaaat gaaagcgcac tgtcaataaa
    19681 gcaggctcct ttcacggttg taacgtagtt tccgcctgaa tcgatacaat cgagtctttc
    19741 ttttcccgga gctgctttgg ggccaacgcc gacgattccg ttctccgcct gcagcataat
    19801 attaacgttt tcggggatgt agtccaccgc catggtaggc aatccaatac caagattaac
    19861 cacttcgcca tctttgaatt cctgggctat tcttctaata atcaattctc tattattcat
    19921 tatctccacc cacctttaca atcgcgtcga caaatattcc gggaacgttg atatgattgg
    19981 ggtcgatttc ccccgctttc acgtattcgt ctgtctgagc aactacatac tcggctgcgg
    20041 tagccatcac caaattgaag ttgcgtgaag agccgttgat gacaaggttc cctgcttcat
    20101 cagctttatg ggctctgata agcgccacat cggctttgag cggtagttcg agcagatatt
    20161 ctctttcgtc aattttgatt ttcttttttc cttcctctac aatggtgcct acacctacgg
    20221 gggttaaaat accacccaaa ccagctccgc ctgcgcgtat tttttcagcg agcgtccctt
    20281 gcgggaagag ttgaacctct gcttccttcg tcatcaggag tcttcccgtt tctggattgg
    20341 agccgatata ggatgcaaaa atccgcttga ctctgccact tttcacaagt ctataaatag
    20401 ataattccgg tgtgcctgtg tcattggaaa tgatcgtaag attatcagcg gaatttgagt
    20461 cgacaagcgc acgcaccagc tcctccggaa tgcctccttg cataaaacct ccaaccatca
    20521 ccgtgtctcc gtctttaatt ttcttaactg cttcttcagc ggagtttact tttttattca
    20581 tggttccgtc tccttatcta tttttaggaa agcaaacttt attggagata atcgtatagg
    20641 actcttccat atggaagcgt taaatcggca ataaaatgtt ttccgccaac ataagtttta
    20701 accggcaaat ctgccacagg agcattgaca tgggggatca agcttaatct ggcaggccct
    20761 gaccaggctc cttttacagt aatattttca agattatatg ccactagctg agcaatttta
    20821 ggagtgccgt ccacatcagg aattaatttt aaattaactt gcgtttttgc gatggcttta
    20881 gccgtttcgt tttcatcaag cacgttatat ttgaaagggg tggttcccat agctaccagg
    20941 acgttattgt aatgcaatgt acccgtcagc gtctctgtat cctcaacgac caggcgaggc
    21001 ttcgcccatt ttttcggaaa tccccagatt tcacgtcccc ccagaatagc aggtgcatta
    21061 tccaaataca tttgggcaac aaaattacac ggttctccct gaaaagtaca cggaatgaca
    21121 ataccgcttt cctcatagct tcccaaaccg gatgaatcgg gcattttgat ccattcatag
    21181 gagaccgtat tacttccatc tggctgaagt ggttccggga ccgcctgtct tatcgcagcg
    21241 ggatcggatt cataggtaat aatgaagaat tcccggttga tgaagcggca tggcgggcgt
    21301 ccataactgc cgctggccgc aggcatggat tgaagtgaaa gtacatcttt ttgattcata
    21361 gcgatctccc ttccttttta agaggtattt caacaatcat tgaacacgaa ctttaaatga
    21421 gtgcattttc aaccagcatt gctattccca ttcccgttcc gatgcataat gagactacgc
    21481 cgtaacgtga agatctgcgc accatttcat gaactaacgt aaccaatacc ctagcaccgc
    21541 ttgcaccaac aggatgaccg attgcgatcg cgccgccatt gacattgacc ttttccggac
    21601 tgactcccag ctctttttgc accgctagtg cttgtgcagc gaacgcttca ttaatttcaa
    21661 agagatcaat gtcatcaatc gacattccct gtttcttcag cagtgacgaa atggctttta
    21721 ccggtcccat tcccatatat gcagggtcaa caccaacaag cgaataaccc ttgatatatg
    21781 ccaacggctt taacgcatgt tccttacatt tgtcttccga cacgactagc atagccgccg
    21841 caccatcatt gatgcccgaa gcgtttcccg cagtaaccgt tccgttctct ttaaacgcag
    21901 gttttaactg agaaagtttt tctttcgtcg tatttcctct tatgtgttcg tcctctgtga
    21961 aataagtagt ttcttttttg tttttgatca tgaccggaac aatttgctct tcaaaaacct
    22021 tgttgttttt tgcttcgaca gcctttatct ggctttcata agcaaatgca tcttgctcaa
    22081 gtcttgaaat tccatatttt tcggcaacat tttcggcggt aattcccata tggtaatggt
    22141 tgatcgggca tgttaggcca tcggccacca aggagtctac caattcaccg ttaccgagct
    22201 tgtagccgtt tctagcattt tttagcagat aaggggcgtt cgacatgctc tccatgccac
    22261 cggccaagac gatgtttcct tgttctgcca atatggaatc gtaagctaac gcgacggcat
    22321 gaagccctga accgcaaacc gtgtttatcg ttgattcggg tacatcaatg gggataccgg
    22381 cttttagtgc cgcctgtctg gcaggatttt gaccattgcc cgcttgaaga acatttccca
    22441 taaaaatttg atcaatcaaa gaaggctcta atttactttg ctgaaggcat gctgtcatca
    22501 cggttgctcc cagttcagga gcgctcaggg gggcaagagc tttattaaat gaaccgaccg
    22561 ctgttctcaa cgggcttact atagctactt ttttcataaa aactcacatt cctttcgctg
    22621 tgttcgagga cacaatacat ccatgactgc acttccattc gtaattccgg tcctaaccta
    22681 cgaaacgtag aaactgcaag atacatttac atgcgggcat atcatggtgc ctaagacagt
    22741 gagtatgacg gtccgatcgc cttctttcct ttctggaaat taaaaccagg attatcatag
    22801 ttctaataca gattttgctc aatgttcaaa tttatacata gatccaaata tagagccata
    22861 tcttccacaa taaataggcc atcctaatgt tttctgttaa tttcttgatt tttctgaaaa
    22921 tcgtgatcaa aaattgaatt ttatgttaaa tatgaaattt caaatcaacc tgtgaaaata
    22981 atttcttagc aaatttcatt tagatcatcg caggggagta aatcccttta tatgtaaatt
    23041 tcgtaactgg cttatatcat ctaaaaaagc taggcaagtt gccaatcaaa atgaatacat
    23101 ttcgaattcg gataaacata aatccatata agcctgagcc acctatagca ttgacgttat
    23161 aacgtcagtg ttttttattt gctcgcgtga acaaaaaaaa gaagccgacc tgcttgaaag
    23221 caatcggctt ggaacgtaaa aaattcactt ctacatttca cgaaaaatgt acatctgccg
    23281 ttgtgagcaa acgtgcaaaa cgattatcta gcacggcatt gtgatgagct acgatttgtt
    23341 cgccgctcag taccttgcta tcaaatgtac tatgcgcatc cgatacgagc acgttgttct
    23401 ggtatcccag actataggcg cttcggcaag tcgtgtcgag acaaaattca gtctgcatcc
    23461 cggcaataat cagctgttcc gcccccaatt cctggagcac atcacgcaat tcagtttggt
    23521 agaagctgtc ccaggatgat tttcggacaa ttcgttcatg aggaagagga cgcaccacgt
    23581 cggcgatggc ccagcctaca ctgttttccc ggaaatcctc atccgcttca tcggtattct
    23641 gcacgaaaat tactggtgtg ccggtagctc tggcttcggt caaaagcaac tgcaaatgcg
    23701 ctacaacttc ctgctcacga tacagcttct catcatacat gaacattgcc tgctgaacat
    23761 caatcaagag taaaacggtt tgtcccatca gtttgattcc ctccctatgt gtaacactta
    23821 acttacaata gcatgtcttc atgccttgcg gcgacataag gtgaactata taataaagaa
    23881 caagttggaa cggtccgatt cggacgaaag gtgattaaaa gatatgacaa tttcgcagga
    23941 acagtttgta ttccgttctg tctcagaggc acagaccggg acattagcag gctttttggc
    24001 agcccaagtg gttcccggaa ccgtgattgt gctggatgga gatctgggag cgggcaagac
    24061 tgcttttt
//
