LOCUS       NZ_JAHTIK010000001     10393 bp    DNA     linear   CON 01-NOV-2023
DEFINITION  Kitasatospora sp. DSM 101779 AL-KSAMP_AS10_SC01, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHTIK010000001
VERSION     NZ_JAHTIK010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Kitasatospora sp. DSM 101779
  ORGANISM  Kitasatospora sp. DSM 101779
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Kitasatospora.
REFERENCE   1  (bases 1 to 8354403)
  AUTHORS   Samborskyy,M., Nasim,S., Bilyk,O. and Luzhetskyy,A.
  TITLE     Kitasatospora nimphea DSM101779 genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8354403)
  AUTHORS   Samborskyy,M., Nasim,S., Bilyk,O. and Luzhetskyy,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2021) DNA Sequencing Facility, Explogen LLC, 20
            Zelena Street, Lviv 79004, Ukraine
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-JAN-2021
            Assembly Method                   :: Newbler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 05:30:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,790
            CDSs (total)                      :: 7,686
            Genes (coding)                    :: 7,384
            CDSs (with protein)               :: 7,384
            Genes (RNA)                       :: 104
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 302
            CDSs (without protein)            :: 302
            Pseudo Genes (ambiguous residues) :: 0 of 302
            Pseudo Genes (frameshifted)       :: 70 of 302
            Pseudo Genes (incomplete)         :: 265 of 302
            Pseudo Genes (internal stop)      :: 17 of 302
            Pseudo Genes (multiple problems)  :: 46 of 302
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:18:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6273155
            Orig. end                         :: 6283548
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHTIK010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="23"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5393)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(426..917)
                     /locus_tag="KSNIM_RS28535"
                     /old_locus_tag="KSNIM_28230"
     CDS             complement(426..917)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSNIM_RS28535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSNIM_28230"
                     /product="hypothetical protein"
                     /protein_id="WP_263105984.1"
                     /transl_table=11
                     /translation="MSVPASATGEPGRSVRAGGRPAVVLWALLVAGLMTLLPCAGTAKA
                     VSGHFPAAPPVSAATGPHAADAVPAAHGAGHAVAGWSDEGGPHVWCSADGDHPGPGKGC
                     TSHPFRGLEAQLPNAPPQPVAVALPQLVPAPALPAAVPLDTLAGPPAAPDLHVLQVHRS
                     "
     misc_feature    complement(973..992)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.37"
                     /tool="antismash"
     misc_feature    994..1010
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     misc_feature    1143..1157
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 15.7"
                     /tool="antismash"
     gene            1225..3135
                     /locus_tag="KSNIM_RS28540"
                     /old_locus_tag="KSNIM_28235"
     CDS             1225..3135
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025053.3"
                     /locus_tag="KSNIM_RS28540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSNIM_28235"
                     /product="protein phosphatase 2C domain-containing protein"
                     /protein_id="WP_263105985.1"
                     /transl_table=11
                     /translation="MSNQGASPHDRPEDGWWQAVYAGPAGTLPDAPGAAGGEGSVDDWF
                     DSAAGMIGPQRTGTPAGPAPEAAPVPEAGAPTHESAPAGAAPKDAPAPSRPHWAPPVKP
                     AQDPAAEPAPRPVPAPTPSRPHWAPPAAAEREPAVPPAARPEPAAAPPRPSWVPLARTG
                     PDPAAAAEPGVDEPGVDEPSVDDWFDMAAGVIGGPRPAPAVAEEQAPSAPEPVDDAEPE
                     PTLTLGTVRRRVPEPAPAAPGPVDGAEPEPTLTLGTVRRPGPEPDTTVDVRPAGRPAAP
                     VTGPTVILRKLASPDADRPESTTSAPARPAQVEPAPAEAAPAQPAAAQPRPAPRRIPLE
                     PPVPHVGERPPTYSPEPTALPASDPAGLGAIVPDTVLDGAQYGPAILRAASVRGDSARF
                     RGEPRRDALLVTRFGEGEDGLVLAVVASPARSADAATGSAAAAEAARQLAAAIGRSRVE
                     LAADLRAGARDRLRYGLQRLTVGAAARLRPAGDPPVEPGDAESGALHCLLVPLDGEAGH
                     RAAFGTGPGGLYLLRSGHWIDAYAARLLHHPDGEPQPAAAAAAPRPFRFRMVPATTGDI
                     LLLCTAGLAGPIADEPAVARFLSNHWAHPHPPGSVDFLRQVQVRAKGYADDRTAAALWT
                     E"
     gene            complement(3164..3790)
                     /locus_tag="KSNIM_RS28545"
                     /old_locus_tag="KSNIM_28240"
     CDS             complement(3164..3790)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135110.1"
                     /locus_tag="KSNIM_RS28545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSNIM_28240"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_263105986.1"
                     /transl_table=11
                     /translation="MAEPRARRAARPREEVFATARAAIAEHGLDKLTMAGLGRELQMSA
                     GHLLYYFGSKDQLLLETLRWSEEQLGGRRRAALARQELTVRQRLAAYAELYLPEGPGDP
                     RWILWVEVWGRSPASAEIRQGQLEIEAPWQADLVALIEEGVASGAFRRPPADGEHGTAG
                     EPHDRARQIRAVLDGFAVPLAVGLPGADRADALAQVASVTAALLD"
     gene            complement(3857..4018)
                     /locus_tag="KSNIM_RS28550"
                     /old_locus_tag="KSNIM_28245"
     CDS             complement(3857..4018)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSNIM_RS28550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSNIM_28245"
                     /product="hypothetical protein"
                     /protein_id="WP_263105987.1"
                     /transl_table=11
                     /translation="MTTHWLITFTDELMRLTDDLDQEQAAEFVRKVYEAGRAAGRQEEA
                     EGQEHSGW"
     gene            complement(4121..4948)
                     /locus_tag="KSNIM_RS28555"
                     /old_locus_tag="KSNIM_28250"
     CDS             complement(4121..4948)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038020.1"
                     /locus_tag="KSNIM_RS28555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSNIM_28250"
                     /product="tyrosinase family protein"
                     /protein_id="WP_263105988.1"
                     /transl_table=11
                     /translation="MAVRKNQATLTAKEKRDFVDALLQLKRLGRYDAYVSTHNRFIVSD
                     TDNGERTGHRSPSFLPWHRKFLLQFEQDLQSVNPAVTLPYWDWTADRTATAGLWADDLL
                     GGNGRSGDNRVTTGPFAGAGGQWTITVRPDSRDYLRRSLGTGVAQLPTRAEVESVLAVQ
                     TYDAAPWNSSSAGFRNQLEGWRGVNLHNRVHVWVGGLMGTGMSPNDPVFWLHHCFIDKL
                     WAEWQRRHPQQGYLPTAATTNVVSLNTAMKPWNDTTPADMLDHTRYYTYDSAF"
     gene            complement(5001..5393)
                     /locus_tag="KSNIM_RS28560"
                     /old_locus_tag="KSNIM_28255"
     CDS             complement(5001..5393)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005952.1"
                     /locus_tag="KSNIM_RS28560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSNIM_28255"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_263105989.1"
                     /sec_met_domain="melC (E-value: 1.2e-45, bitscore: 153.9,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAGHHAELTRRRMLQGAGVALTAAVGTAVLGLAGPTGTPAAAA
                     GPGGHDTFDEVYRGRRIQGRPAASGHHHHGADYTVQVDGEELHVMRNADGTWISVVNHY
                     QPHQTPLALARAAVVELQGATLVPIA"
     misc_feature    5411..5424
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.49"
                     /tool="antismash"
     gene            complement(5626..6417)
                     /locus_tag="KSNIM_RS28565"
                     /old_locus_tag="KSNIM_28260"
     CDS             complement(5626..6417)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361039.1"
                     /locus_tag="KSNIM_RS28565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSNIM_28260"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_263105990.1"
                     /transl_table=11
                     /translation="MSQDSSPPPYLRVAERLRQRIQSGDLAPGDLLPSLSEIKEETGYS
                     GTVGQRAYAVLVDEGLVVARPGRGHFVRSQEERPLLVRRPRVAAGSGSPTEALLTAQGV
                     RASWDSESTTARADARIAERLGIAPGDPVMHTAYVYRADGEPVQLADSWEPMAVTGGTL
                     VVLPEAGPYAGIGVADRMALIGIEVGTPVERVTARGASRAEAALLGSTPAAPVLAVERT
                     YYDQAAGRAVETADIVLLGSRWAAEYGTRPTTADGPTGTQG"
     misc_feature    complement(6436..6451)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     misc_feature    complement(6476..6492)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.58"
                     /tool="antismash"
     gene            6514..6756
                     /locus_tag="KSNIM_RS28570"
                     /old_locus_tag="KSNIM_28265"
     CDS             6514..6756
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSNIM_RS28570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSNIM_28265"
                     /product="hypothetical protein"
                     /protein_id="WP_263105991.1"
                     /transl_table=11
                     /translation="MQVSVRANVLREALERATGEVATTTGKAPGAVRITVTIPERCTDE
                     QWAAVLQVLRSVARWGASDGAGRTVVWAEVQEDPC"
     gene            6750..6983
                     /locus_tag="KSNIM_RS28575"
                     /old_locus_tag="KSNIM_28270"
     CDS             6750..6983
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KSNIM_RS28575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KSNIM_28270"
                     /product="hypothetical protein"
                     /protein_id="WP_263105992.1"
                     /transl_table=11
                     /translation="MLKPNPDPDRDSADSADSAGPRESREALPEPDEVPRYRRCGRCAR
                     CHGGGPGAPPTGDCWSDYLDAVTEVLTRAAGG"
     gene            complement(7082..8119)
                     /locus_tag="KSNIM_RS28580"
                     /old_locus_tag="KSNIM_28275"
     CDS             complement(7082..8119)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554520.1"
                     /locus_tag="KSNIM_RS28580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSNIM_28275"
                     /product="agmatine deiminase family protein"
                     /protein_id="WP_263105993.1"
                     /transl_table=11
                     /translation="MTTPAELGFRMPAEWHPHERTWMAFPTTNPTFDSPEHLHAGREAW
                     AAVANTIVRYEPVTLVVNTGETEEARKYVAPEVEIVERPLDDAWMRDIGPTFLVDGKGG
                     VAAADWIFNGWGAQSWARWDNDQHIAEHITGLAGVQRFASRLINEGGGIHVDGEGTVLV
                     TDTVQLGEGRNSDWTREEVEAELHAFLGTTKAIWLPRGLTRDYDEFGTRGHIDIVASFV
                     RPGVVVAHVQPDPAHPDHEVCRELVAILRASTDAQGRPLEVIELPAPTVLLDEEGEPVD
                     YSYINHYVANGAVVLCAFDDPRDQEAAAILAEAYPGRTVELVDAREIFANGGGIHCITQ
                     QQPQG"
     gene            complement(8154..9878)
                     /locus_tag="KSNIM_RS28585"
                     /old_locus_tag="KSNIM_28280"
     CDS             complement(8154..9878)
                     /EC_number="3.5.1.5"
                     /GO_function="GO:0009039 - urease activity [Evidence IEA]"
                     /GO_process="GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018570054.1"
                     /locus_tag="KSNIM_RS28585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KSNIM_28280"
                     /product="urease subunit alpha"
                     /protein_id="WP_263105995.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.1e-44, bitscore:
                     151.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAISPHDYISVHGARAGDRIRLGDSGLIVRVESDSQEPGNEFLA
                     GFGKTARDGLHLKATAIRETCDVVISNVVVIDAVQGIRKTSIGIRDGRIVSVGRAGNPD
                     TMDGVDVVVSTGTTIVSGEGMIATAGAIDTHVHLLSPRIMEASLASGVTTIIGQEFGPV
                     WGVGVNSPWALRHAFNAFDAWPVNIGFLGRGSSSDAAPLIEALAEGGASGFKVHEDMGA
                     HTRALDTALRVAEEYDVQVALHTDGLNECLSVEDTIKVLEGRTIHAFHIEGCGGGHVPN
                     VLKMAGVENVIGSSTNPTLPFGRDALGEHFGMIVSAHDLKVDLPGDAAMARDRIRAGTM
                     GAEDVLHDLGVIGITSSDAQGMGRAGETVRRTFAMAGKMKAELGPMDGEGSYGTTAGGD
                     DNERVLRYIAKLTINPAIAHGLSHEVGSLEVGKLADIVLWRADHFGAKPQLVLKAGFPA
                     YGVVGDPNASTDRCEPLVLGPQFGAHGATAADISVAFVSQAAAEASYLDVAGDQLPTRR
                     RRVGVRGTRGIGPKDMVRNARTGHVQVEAATGLVSLDGEPLRSDPADSVSLSRLYFL"
     misc_feature    9865..9876
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.68"
                     /tool="antismash"
ORIGIN
        1 gcgacaggtt gtcccaggtc tgcacgccct ggatcggggc cttggcggca gcctccttcg
       61 cgtccttgtc gtgcttggcg tcgagggcta tccaggtccc ggcgccgatc atgccggcca
      121 gcagcacgcc ggccaggccg aagatgacga tcttgttgcg gcggtcgcgc ttctgctcgg
      181 cggcacgcag ctccgcgatg cgggcccggc ggtcggcctg ccgctcgttc gggctctgcc
      241 ggttgcccgg gttgccggag gtctgcttgg acggcttgga ctgcctggag gcggaaccca
      301 tgagtgtgtc cttgtctccg cggtgcgcac gggcacggcg gtagggcgag agggggaggg
      361 ggcggcggag ccgtcggccc gtccggtgca caggggcacg ggtcacggcc gacggtgggt
      421 tccgctcagg acctgtggac ctgcagcacg tggaggtcgg gcgcagcggg cggcccggcc
      481 agcgtgtcga gcgggacggc ggcgggcagc gccggcgccg gaaccagctg cggcagggcg
      541 accgccaccg gctgcggggg agcgttcggc aactgcgcct ccagcccgcg gaacgggtgg
      601 ctggtgcagc ccttccccgg accaggatga tcgccgtcgg cggagcacca gacgtgcgga
      661 ccgccctcgt cggaccaccc ggcgacggca tgtccggcgc cgtgggcggc cgggacggcg
      721 tcggcggcgt gcggaccggt tgcggccgag actggagggg cggccgggaa gtgccctgac
      781 accgccttcg ccgtcccggc gcacggcaac agcgtcatca gaccggcgac gagcagcgcc
      841 cacagcacca cggccgggcg accgcccgcg cggacggacc gccccggctc gccggtcgcg
      901 gatgctggca cgctcatgcg cacatattaa tcagccgaag gggtgaagcg gtccccctct
      961 tggcccaccc gacccgggcc ggaaggcccc tcctgcgcat ttcaggggcc caaaaaggac
     1021 gctccggaca tccgcccccg acccgtccgc gccccctccg gacggatggc cgaatccgag
     1081 cggatcacac cgtcgctgcg cccggcgggc ggccgggtgt ccccgaaagc cggacaccgt
     1141 cgttctcctt cggaagaatg accaaccgag tccggcacac cggcccgctc ggcagacggc
     1201 agacacggca cgaggagccg ccacgtgagc aaccagggcg catccccgca cgaccggccc
     1261 gaggacggct ggtggcaggc ggtctacgcg ggcccggcgg gcaccctgcc ggatgctccc
     1321 ggggccgccg ggggcgaggg cagcgtcgac gactggttcg actccgcggc cggcatgatc
     1381 ggcccccagc gcaccggcac ccccgccggc ccggctccgg aggccgcccc ggtacctgag
     1441 gcgggcgcac cgacacacga gtcggcacca gcgggcgccg caccgaagga cgcaccggcc
     1501 ccgtcccgcc cgcactgggc gccaccggtg aagccggcgc aggacccggc ggcggagccc
     1561 gcgccgcggc cggtgccggc accgacgccg tcccgcccgc actgggcacc gccggcggcc
     1621 gcggaacggg agcccgccgt accgcccgcc gcccggccgg agcccgcagc ggccccgccc
     1681 cgcccgagct gggtacccct cgcccgtacc ggcccggatc ccgctgccgc agcggagccc
     1741 ggcgtcgacg agcccggcgt cgacgagccc agtgtcgacg actggttcga catggccgcc
     1801 ggggtgatcg gcggcccgcg ccccgccccg gccgtcgccg aggagcaggc cccgtccgct
     1861 cccgagcccg tcgacgacgc cgagcccgag ccgacgctca cgctcggaac ggtccgccgc
     1921 cgcgtgccgg agcccgcccc cgcggcgccc gggcccgtcg acggcgccga gcccgagccg
     1981 acgctcacgc tgggtaccgt ccggcggccc gggccggagc cggacaccac cgtcgacgtg
     2041 cgtcccgccg gacggcccgc cgcccccgtg accggtccga cggtgatcct gcgcaagctc
     2101 gcgtcccccg acgcggaccg gcccgagtcg acgacgtccg ccccggcccg acccgcccag
     2161 gtggagcccg ctccggccga agccgccccg gcgcagcccg ccgcggccca gccccgtccc
     2221 gccccgcgcc ggatcccgct ggaaccgccg gtcccgcacg tgggcgagcg gcccccgacg
     2281 tacagcccgg agcccaccgc gctgccggcc tccgatccgg ccgggctggg tgcgatcgtt
     2341 ccggacaccg tgctcgacgg cgcccagtac ggcccggcga tactgcgggc ggcctccgtc
     2401 cgcggcgact ccgcccggtt ccgcggcgaa ccgcgccgcg acgccctgct ggtgacccgg
     2461 ttcggcgagg gcgaggacgg cctggtgctc gcggtggtcg cctcgcccgc ccgctcggcg
     2521 gacgccgcca ccggctcggc cgccgccgcg gaggccgccc ggcagctcgc cgccgcgatc
     2581 ggccgcagcc gggtcgaact cgccgcggac ctgcgcgccg gggcccgcga ccggctccgc
     2641 tacggcctgc agcggctcac cgtcggtgcg gccgcccgcc tccggccggc cggcgacccg
     2701 ccggtggagc ccggcgacgc ggagtccggc gcgctgcact gcctgctcgt cccgctggac
     2761 ggcgaggccg ggcaccgggc cgcgttcggc accggccccg gcgggctcta cctgctgcgt
     2821 tccggccact ggatcgacgc ctacgcggcc cggctgctgc accaccccga cggggagccg
     2881 cagccggcgg ccgccgccgc ggcgccccgg ccgttccggt tccggatggt gcccgcgacg
     2941 accggtgaca tcctgctgct ctgcacggcg ggcctggccg gcccgatcgc ggacgagccc
     3001 gcggtggcgc gtttcctctc caaccactgg gcccacccgc acccgccggg cagtgtcgac
     3061 ttcctgcgcc aggtccaggt gcgggccaag ggctacgccg acgaccgcac cgccgccgcg
     3121 ctctggacgg agtagcggcg gcccgccgcc ccgtgaccgc cgctcagtcg agcagggcgg
     3181 cggtcacgga ggcgacctgg gccagggcat cggcgcggtc ggcccccggc aggccgacgg
     3241 ccagcgggac ggcgaagccg tcgagcacgg cgcggatctg ccgtgcccgg tcgtgcggtt
     3301 cccccgccgt gccgtgctcg ccgtcggcgg gcggacggcg gaaggcgccg gaggcgacgc
     3361 cctcctcgat cagcgcgacg aggtcggcct gccagggagc ctcgatctcc agctggccct
     3421 gccggatctc ggcgctggcg ggcgagcggc cccagacctc gacccagagg atccaccggg
     3481 gatcgccggg cccctcgggc aggtacagct cggcgtaggc ggcgagccgc tgccggacgg
     3541 tgagctcctg ccgggccagg gcggcgcgcc gccgcccgcc gagctgctcc tcgctccagc
     3601 ggagggtctc cagcagcagc tggtccttgc tgccgaagta gtagaggagg tggccggcgc
     3661 tcatctgcag ctcgcggccg agaccggcca tggtgagttt gtcgagtccg tgctcggcga
     3721 tcgcggcccg ggcggtcgcg aagacctcct cgcggggccg tgcggcccgg cgcgcccgcg
     3781 gctccgccat gtcctgctcc tcctcgtcgt ccggccccac tttagggctt cgccaccggg
     3841 ccgtcggcga gcggtctcac cagccgctgt gctcctggcc ctcggcctcc tcctgtcggc
     3901 cggcggcgcg gccggcctcg tacaccttgc gcacgaactc ggcagcctgc tcctggtcga
     3961 ggtcgtcggt gaggcgcatc agctcgtcgg tgaaggtgat cagccagtgt gtggtcatgc
     4021 cctcatgaca ccgtgcggga cggcgcgcag cccgccggac accgtccggc gggctgcgcg
     4081 tcctgcacaa tcactcgtcc ggccggggcc ggagcggcgg tcagaacgcc gagtcgtagg
     4141 tgtagtaccg ggtgtggtcg agcatgtcgg ccggggtggt gtcgttccac ggcttcatcg
     4201 cggtgttcag cgagaccacg ttggtggtgg cggcggtcgg caggtagccc tgctgggggt
     4261 ggcggcgctg ccactcggcc cacagcttgt cgatgaagca gtggtgcagc cagaacaccg
     4321 ggtcgttggg ggacatgccg gtgcccatca gaccgccgac ccagacgtgc acccggttgt
     4381 gcaggttgac gccgcgccag ccctccagct ggttgcggaa gcctgcggag gagctgttcc
     4441 acggggcggc gtcgtaggtc tggacggcga ggacggactc cacctcggcc cgggtgggca
     4501 gttgggcgac gccggtgccg agcgagcggc gcaggtagtc gcggctgtcg gggcggacgg
     4561 tgatcgtcca ctggccgccg gcgccggcga acgggccggt ggtgacgcgg ttgtcgccgg
     4621 agcggccgtt gccgccgagc aggtcgtcgg cccacaggcc ggcggtggcg gtgcggtcgg
     4681 ccgtccagtc ccagtagggc agggtgaccg cggggttgac ggactgcagg tcctgctcga
     4741 actggagcag gaacttgcgg tgccagggca ggaacgacgg cgagcggtgg cccgtccgct
     4801 cgccgttgtc ggtgtcgctg acgatgaagc ggttgtgggt gctgacgtag gcgtcgtagc
     4861 ggccgagccg cttgagctgg agcagggcgt cgacgaagtc gcgcttctcc ttggccgtga
     4921 gggtcgcctg gttcttgcgg accgccatgg tgtcctcctg gaggtgctgg tgggtgggtg
     4981 cgggggaggg gcgggcccgg tcaggcgatc gggacgaggg tggcgccctg cagctcgacg
     5041 acggcggcgc gggcgagcgc gagcggggtc tggtgcggct ggtagtggtt gacgacgctg
     5101 atccaggtcc cgtcggcgtt gcgcatgacg tgcagttcct cgccgtcgac ctgcacggtg
     5161 tagtcggcgc cgtggtggtg gtggccggat gcggcggggc ggccctggat gcggcggccg
     5221 cggtagacct cgtcgaaggt gtcgtggccg ccgggcccgg ccgcggcggc cggggttccg
     5281 gtcggtccgg cgaggccgag gacggcggtg ccgacggcgg cggtgagggc gacgccggcg
     5341 ccctggagca tccggcggcg ggtcagctcg gcgtggtgac cggcttcggg catgggtgat
     5401 tcccccctgg tggagcggat ctgacgacgc gtcggcggcg gcacgtgctt gtggcatatg
     5461 cgccgtcgcg cggtccccat gctggcaagg cgggccgcgg ccgcggaacc gtccatcggc
     5521 gggggccggt cgacagaccg aaatccggac acgggtgaac gctcaacgaa caccgtgggc
     5581 cgcgcccgac ggagggctga cggcgcgtcg gcccgggccg ggcgatcagc cctgcgtgcc
     5641 ggtgggcccg tccgcggtgg tgggcctggt gccgtactcg gcggcccagc ggctgccgag
     5701 cagcacgatg tcggcggtct ccactgcgcg gccggcggcc tggtcgtagt aggtgcgctc
     5761 gacggccagg acgggggccg cgggcgtgct gccgagcagg gcggcctcgg cgcgcgaggc
     5821 gccgcgggcg gtgacccgct cgaccggggt gccgacctcg atcccgatca gcgccatccg
     5881 gtcggcgacg ccgatgcccg cgtaggggcc ggcctccggc aggaccacca gggtgccgcc
     5941 ggtgacggcc atcggctccc aggagtcggc cagttggacc ggctcgccgt cggcccggta
     6001 gacgtacgcg gtgtgcatca ccgggtcgcc gggggcgatg ccgaggcgtt ccgcgatccg
     6061 cgcgtcggcc cgggcggtgg tcgactcgct gtcccaactc gcccggacgc cctgggcggt
     6121 gagcagtgcc tcggtcggcg atccggagcc ggcggcgacc cgtgggcggc gcaccagcag
     6181 ggggcgttcc tcctgcgagc ggacgaagtg gccgcggccc ggccgcgcga cgaccaggcc
     6241 ctcgtcgacg agcacggcgt acgcgcgctg gccgacggtg ccggagtacc cggtctcctc
     6301 cttgatctcg gagagggagg gcaggaggtc accgggcgcg aggtcgccgg actggatgcg
     6361 ctggcggagc cgctcggcca cccggaggta cgggggcggc gagctgtcct gactcatcct
     6421 gtgtcctctc actgagtgac ggcgaggtga cagagtatcc gtccggccac tagtctatta
     6481 gtacagttcc agtctgttac tagggaaggg gcggtgcagg tcagcgtgcg tgcgaacgtc
     6541 ctgcgcgaag ccctcgaacg ggccaccggc gaggtcgcga cgaccaccgg gaaagcccct
     6601 ggcgctgtcc gcatcaccgt gacgatcccg gagcggtgca ccgacgagca gtgggccgcc
     6661 gtgctgcagg tcctgagatc agtggcgcgc tggggcgcca gtgacggcgc cggtcgcacc
     6721 gtggtctggg ccgaggtcca ggaggacccg tgctgaagcc gaaccccgat cccgaccgcg
     6781 actccgccga ctccgcggat tccgccggtc cgcgcgagtc ccgcgaagcc ctgcccgagc
     6841 cggacgaggt gccgcgctac cggcgctgcg ggcggtgcgc ccgctgccac ggcggcggcc
     6901 cgggtgctcc gccgaccggc gactgctggt ccgactacct ggacgccgtc accgaggtgc
     6961 tcacccgcgc cgccggcggc tgaatccgac cgggcggggc cgtcccggtg cagcaacgcc
     7021 ggggcgggcg cggcacctcg cggtaccgcg ccggccccgg cgtgcgacag ccgaccctgc
     7081 gtcagccctg cggctgctgc tgggtgatgc agtggatacc gccgccgttg gcgaagatct
     7141 cccgggcgtc gaccagctcc accgtgcggc ccgggtaggc ctcggcgagg atcgcggccg
     7201 cctcctggtc gcgcgggtcg tcgaaggcgc agagcaccac ggcgccgttc gccacgtagt
     7261 ggttgatgta cgagtagtcg accggctcgc cctcctcgtc cagcagcacc gtgggcgcgg
     7321 gcagttcgat cacctcgagc ggccggccct gcgcgtcggt ggaggcgcgc aggatcgcca
     7381 ccagctcccg gcagacctcg tggtccgggt gggccgggtc gggctgcacg tgtgccacga
     7441 ccacgccggg gcggacgaag gaggcgacga tgtcgatgtg cccgcgggtg ccgaactcgt
     7501 cgtagtcgcg ggtgagtccg cgcggcagcc agatcgcctt cgtggtgccg aggaaggcgt
     7561 gcagctcggc ctcgacctcc tcgcgggtcc agtcggagtt gcggccctcg cccagctgca
     7621 cggtgtcggt gaccaggacg gtgccctcgc cgtcgacgtg gatgccgccg ccctcgttga
     7681 tcagccggga ggcgaagcgc tgcacaccgg cgagcccggt gatgtgctcg gcgatgtgct
     7741 ggtcgttgtc ccagcgggcc caggactgcg cgccccagcc gttgaagatc cagtccgcgg
     7801 cggccacccc gcccttgccg tcgaccagga aggtcgggcc gatgtcgcgc atccacgcgt
     7861 cgtcgagcgg ccgttccacg atctccacct cgggggcgac gtacttgcgc gcctcctcgg
     7921 tctcgccggt gttgacgacc agcgtcaccg gctcgtaccg gacgatggtg ttggcgacgg
     7981 cggcccaggc ctcgcggccg gcgtgcaggt gttcggggct gtcgaaggtg gggttggtgg
     8041 tcgggaaggc catccaggtg cgctcgtgcg ggtgccattc ggcgggcatc cggaatccga
     8101 gctcggcggg agtggtcatg agggtgcgtt cctaagcggt ggtggggtgg gggtcagagg
     8161 aagtagaggc ggctgagcga gacgctgtcg gcggggtcgg agcgcagcgg ttcgccgtcc
     8221 agcgagacca ggccggtggc ggcctccacc tggacgtggc cggtccgggc gttgcgcacc
     8281 atgtccttcg gaccgatccc gcgggtgccc cggacaccca cccggcgcct gcgggtgggc
     8341 agttggtcac cggcgacgtc caggtaggag gcctcggcgg cggcctgcga gacgaaggcg
     8401 accgagatgt ccgcggcggt ggcgccgtgc gcgccgaact gcggcccgag gacgagcggt
     8461 tcgcagcggt cggtggacgc gttggggtcg ccgaccacgc cgtacgcggg gaagcccgcc
     8521 ttgaggacca gttgcggctt ggcaccgaag tggtcggccc gccacagcac gatgtcggcg
     8581 agcttgccga cctccagcga gcccacctcg tgggagaggc cgtgggcgat ggccgggttg
     8641 atggtgagtt tggcgatgta gcgcagcacg cgctcgttgt cgtcgccgcc ggcggtggtg
     8701 ccgtacgagc cctcgccgtc catcgggccg agttcggcct tcatcttgcc ggccatggcg
     8761 aaggtgcggc gcaccgtctc gccggcccgg cccatgccct gggcgtcgga ggaggtgatg
     8821 ccgatgaccc cgaggtcgtg cagcacgtcc tcggcgccca tcgtgcccgc ccggatccgg
     8881 tcgcgggcca tcgcggcgtc gcccggcagg tcgaccttga ggtcgtgcgc ggagacgatc
     8941 atgccgaagt gctcgccgag ggcgtcccgg ccgaagggca gggtggggtt ggtggaggag
     9001 ccgatgacgt tctccacacc ggccatcttc agcacgttgg ggacgtgccc gccgccgcag
     9061 ccctcgatgt ggaaggcgtg gatggtgcgg ccctccagca ccttgatggt gtcctcgacg
     9121 gagaggcact cgttcaggcc gtcggtgtgc agggcgacct ggacgtcgta ctcctcggcg
     9181 acccgcagtg cggtgtccag ggcgcgggtg tgcgcgccca tgtcctcgtg caccttgaag
     9241 ccggaggcgc cgccttcggc gagcgcctcg atcagcgggg ctgcgtcgga ggaggagccg
     9301 cggccgagga agccgatgtt gaccggccag gcgtcgaagg cgttgaaggc gtgccgcagc
     9361 gcccaggggg agttgacgcc gacgccccag accgggccga actcctggcc gatgatcgtg
     9421 gtgacgcctg aggccagcga ggcctccatg atccgcggcg agagcaggtg gacgtgggtg
     9481 tcgatggcgc cggcggtggc gatcatgccc tcgccggaga cgatcgtggt gccggtggag
     9541 acgacgacgt ccacaccgtc catggtgtcc gggttgccgg cccgtccgac ggagacgatc
     9601 cggccgtccc ggatgccgat cgaggtcttg cggatgccct ggaccgcgtc gatgaccacg
     9661 acgttgctga tcaccacgtc gcaggtctcg cggatcgcgg tggccttgag gtgcaggccg
     9721 tcccgcgcgg tcttgccgaa gccggcgagg aactcgttgc cgggctcctg cgagtcggac
     9781 tcgacccgga cgatcagccc gctgtcgccg agccggatcc ggtcgcccgc ccgtgcgccg
     9841 tgcacggaga tgtagtcatg gggggaaatc gctgtcatga ctcgacctcc gcgtcgtcga
     9901 agccggtgag gtagccggtg gcgcgcgcct tctccagtgc ggcctccttg gcgccgggcg
     9961 cgtccagcgg cccgtcgacc agcccggcga atccgatcgc gacccgctcg ccgccgatcg
    10021 ggacgaggcc gacctcgacg gtggccccgg ggtcgaaccg caccgaggac ccggccggca
    10081 cggccaggtg cgtgccgtag gcggccgcgc ggtcgaaggc gagacgcggg ttggcctcga
    10141 agaagtggaa gtgcgaggtg accgagatcg gcaccgccgc ggtgttgtgg acgggcagca
    10201 cgaccgtctc ctcgacgggg tcgtagccgt gccccgggcc caccagggcc gcgccgggcg
    10261 cctcgtcgcc gagtgagccg gaaccgcgga acgggtcgtt gaccacggcg agccgggtgc
    10321 cgtcgtcgaa gaccgcctcg acctgcacca cggtgaccac gtcgggcacg ccgggcagga
    10381 cgtcgtcggc gga
//
