LOCUS       NZ_CP106799            10945 bp    DNA     linear   CON 01-NOV-2023
DEFINITION  Streptomyces sp. HUAS 15-9 plasmid punmamed2, complete sequence.
ACCESSION   NZ_CP106799
VERSION     NZ_CP106799.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. HUAS 15-9
  ORGANISM  Streptomyces sp. HUAS 15-9
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 249478)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 169.8x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 03:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,774
            CDSs (total)                      :: 8,679
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 504
            CDSs (without protein)            :: 504
            Pseudo Genes (ambiguous residues) :: 0 of 504
            Pseudo Genes (frameshifted)       :: 260 of 504
            Pseudo Genes (incomplete)         :: 328 of 504
            Pseudo Genes (internal stop)      :: 29 of 504
            Pseudo Genes (multiple problems)  :: 107 of 504
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:51:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 187126
            Orig. end                         :: 198071
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106799.1.
            Bacteria and source DNA available from Mo Ping.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5945
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(1060..1725)
                     /locus_tag="N8I87_RS43125"
                     /old_locus_tag="N8I87_43125"
     CDS             complement(1060..1725)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047020.1"
                     /locus_tag="N8I87_RS43125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_43125"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_263217309.1"
                     /transl_table=11
                     /translation="MAQQERAVRTRRAVIEAAATVFAERGYTAATIAEILERAGVTKGA
                     LYFHFDSKEALARGVIGAQIENDYWVPRELKLQEWVDIGMTLAHQIPLDVILLAGIRLS
                     GDMQGRDLFGSAWPAWIGLSTKLLTEAKERGEVLPHVVPQETAECFLGSWLGTQMFSEA
                     NSNWADLSERVSVLYNHMLPAIATPAALIRLDTAPDRGARVIKEAERVRQAALAASEA"
     misc_feature    1738..1751
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.89"
                     /tool="antismash"
     gene            complement(1905..2678)
                     /locus_tag="N8I87_RS43130"
                     /old_locus_tag="N8I87_43130"
     CDS             complement(1905..2678)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (12-171). E-value: 1.3e-14.
                     Score: 46.4. Matches aSDomain:
                     nrpspksdomains_N8I87_RS43130_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999105.1"
                     /locus_tag="N8I87_RS43130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_43130"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_263217311.1"
                     /sec_met_domain="adh_short (E-value: 4.6e-37, bitscore:
                     121.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.1e-30, bitscore:
                     101.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGAGTAPLAGKVVLITGASSGIGAAAGRVFARQGAVVVLTARRE
                     DRLRDLVAQLRGKGAEASYHAMDVRRGEQVERAVRHTIERYGRLDAAFNNAGVGVEKVP
                     LHLLEDEAYELVMETNVRGVWNCMRHEITAMVQGGGGAIVNTSSVGGLVASSTAAPYIA
                     SKHAVIGLTKAAAVEYAAHRIRVNTIAPGLTRSEMVDEWFAVSPAAAEKAYRSAPQGRA
                     AEPEEVAETAAWLCSDRASFVTGATLPVDGGRTAW"
     aSDomain        complement(2166..2642)
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N8I87_RS43130_PKS_KR.1"
                     /evalue="1.30E-14"
                     /label="N8I87_RS43130_PKS_KR.1"
                     /locus_tag="N8I87_RS43130"
                     /protein_end="171"
                     /protein_start="12"
                     /score="46.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVLITGASSGIGAAAGRVFARQGAVVVLTARREDRLRDLVAQLRG
                     KGAEASYHAMDVRRGEQVERAVRHTIERYGRLDAAFNNAGVGVEKVPLHLLEDEAYELV
                     METNVRGVWNCMRHEITAMVQGGGGAIVNTSSVGGLVASSTAAPYIASKHAVIGL"
     CDS_motif       complement(2547..2642)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N8I87_RS43130_0001"
                     /evalue="2.20E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="N8I87_RS43130"
                     /protein_end="44"
                     /protein_start="12"
                     /score="10.7"
                     /tool="antismash"
                     /translation="VVLITGASSGIGAAAGRVFARQGAVVVLTARR"
     gene            complement(2675..3427)
                     /locus_tag="N8I87_RS43135"
                     /old_locus_tag="N8I87_43135"
     CDS             complement(2675..3427)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018554472.1"
                     /locus_tag="N8I87_RS43135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_43135"
                     /product="HAD family phosphatase"
                     /protein_id="WP_263217313.1"
                     /transl_table=11
                     /translation="MSHPALSTLRLAAVNIDGVLLNDTFSPVMHRLVAAQGVEYTPELE
                     RALLSRPQLEAAQVFVEVTGTSATPLEVLGAYFAERERYLREQPVRLLDGAVALLERLR
                     ALGLDLVCYGGLDESHFQRHLGPWAAYFSEPGYICTNAFRPGIREITRDFFSLAPHQAL
                     FIDDAASVAEKARELDVPFIGHPSPFAHGFQQELMRRAGVRYLVRELAAIDEAMLRALD
                     AEAAGRTVWRHGEPTPPQPAAEAPGARS"
     gene            complement(3464..4699)
                     /locus_tag="N8I87_RS43140"
                     /old_locus_tag="N8I87_43140"
     CDS             complement(3464..4699)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211053.1"
                     /locus_tag="N8I87_RS43140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_43140"
                     /product="cytochrome P450"
                     /protein_id="WP_263217315.1"
                     /sec_met_domain="p450 (E-value: 3.9e-27, bitscore: 89.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQNPSVPVVLDPSGQDLHGEAARLRRLGAVVQVALPGGVHAWSVN
                     RGDLIKQVLAEPRVSKDAYRHWPAWINGEVDDSWPLAMWVSVRSMLTAYGEEHTRLRKL
                     VAGAFTARRTVDLRPRIAEITDELLTRLEGLPSAQPVDIRKEFAYELPIRVISETLGLD
                     ARERDDLHRVTDVLFTTTTTPETAQKNQAELYGLMNDLVGRKRVHPGTDLTSALITMRD
                     EDGEGLTEKELVDTLLLIIGAGHETTVNLLDHAICALLTHPEQLRLVREGTVTWEAVVH
                     ETLRSQAPVANILLRFAVEDLDLDGTVIRAGEPILLALAAAGRDPGAHGRDADTFDATR
                     PTRHEHFSFGHGVHHCLGRPLAMSEATIALPALFERFPHLELAVEPRELGLFESFISSG
                     HTELPVLLSPRS"
     misc_feature    4753..4762
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.17"
                     /tool="antismash"
     misc_feature    4790..4804
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     misc_feature    4846..4862
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.74"
                     /tool="antismash"
     gene            5001..5945
                     /locus_tag="N8I87_RS43145"
                     /old_locus_tag="N8I87_43145"
     CDS             5001..5945
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569591.1"
                     /locus_tag="N8I87_RS43145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_43145"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_263217317.1"
                     /sec_met_domain="AfsA (E-value: 7.2e-24, bitscore: 77.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLPTSQSTVELPALPRLTTTVPREYVHRASLAEVFLTGCTAQDD
                     LRFSLTGQWPRAHTLFTSPDGSSHDPLLTAETFRQAGMFLAHAELGVPLHHRFVLGDIN
                     YTTALQNLRIALSPTDFDLEARCTEVTWRRGMASWLQLELSIRRQGNVVAHGSGSFCVI
                     QQGVYKRLRAARKPWSGPAFDIQADRAPLPPATVGRGSTADVVLTATDSPTSWLLTPRL
                     DHPILFDHADDHLPGMVLLEGARQAASALVAPRTMTPASASSSFRRYVELDRPCWIDIT
                     RVSPESGGMMTVEVTGRQDDETVFTSTITGPVG"
     gene            complement(6372..6773)
                     /locus_tag="N8I87_RS43150"
                     /old_locus_tag="N8I87_43150"
     CDS             complement(6372..6773)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668731.1"
                     /locus_tag="N8I87_RS43150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_43150"
                     /product="DUF6009 family protein"
                     /protein_id="WP_263217319.1"
                     /transl_table=11
                     /translation="MSALIGEDEIIHEVDFVWLEDITELDYVRQSLDRLPTRKGRPAYH
                     RDGRMVGYALLGPSAKPSRSSGTFRRRVFWLLPHDRDAVPDGLYATGAPAEAVDPRTLA
                     PGKKGRKTERSEGGPTSPEVREGGLTLPL"
     gene            complement(6770..8281)
                     /locus_tag="N8I87_RS43155"
                     /old_locus_tag="N8I87_43155"
     CDS             complement(6770..8281)
                     /GO_function="GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158409.1"
                     /locus_tag="N8I87_RS43155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_43155"
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="WP_263217320.1"
                     /transl_table=11
                     /translation="MTPDDNALFDAQAVAAQILAQPGAQLPRQAVEPRHGTGGDGEATA
                     DGLLPDTLSDRGNAKLFVKLYSNDYRHVPGLGWYRWDSTRWQIDEDDTVMWAAGDLAES
                     LATDDPRGAHSASALQQHRRRALSTSGMNAMLTQARSAPGMVLNAARLDADPYALCTPA
                     GIVDLRTGLIRTPDPDKDFHSRSTTAGPQAMPTPRWDRFLTDTFGDGPEGAEMIDFLHL
                     LLGYSITGDVGGQVMPFLFGSGKNGKSVLLDVLMKLVGDYADAAPPGFLMARPYEGHPT
                     DLAELHGRRVIVCSEVKPGDRFDEARVKLLTGGDRIKARRMRQDFFSFQPTHKLWLLGN
                     HRPEVGTGGFAFWRRMRLIPFERVVSDDRKIDNLADMLVTEEGPGILNWLILGARRYLR
                     GEKDLAGPERVRIATTAYAETEDHTGRFLSEECCIGPDLRAEQAQLYAAYKTWCQNEGA
                     PAISSRAFAARVREVVGLASPKEMILSNQRKYYPGIGLVADEETA"
     gene            complement(8278..9389)
                     /locus_tag="N8I87_RS43160"
                     /old_locus_tag="N8I87_43160"
                     /pseudo=""
     CDS             complement(8278..9389)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742102.1"
                     /locus_tag="N8I87_RS43160"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_43160"
                     /product="bifunctional DNA primase/polymerase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPAELRVPRSDSRQPSPEPRDAARSLATARWCVGRGWPVHPLAPG
                     RKTPAGNCEACREPGHTHRDCGCPAAGRWCHGFHAATLDYARVEQWWGANPRFGVGIAC
                     GPASLVVVDIDAHAQELPGRDRLLPGIEISAGVDLTGLANGFHTMGVLAALRGEQSPAD
                     DENTLRVRTPSGGLHVWYRAQGGQRWQCSTGSGGGRSLAWQVDVRAHGGYIVAPGTVTA
                     AGVYEPVGPVREPAPLPGWLARELERTGHLPPAHVPVPRPVPPRARQAVLAAGEGTRPR
                     GGP"
     gene            complement(10008..10883)
                     /locus_tag="N8I87_RS43165"
                     /old_locus_tag="N8I87_43165"
     CDS             complement(10008..10883)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N8I87_RS43165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="N8I87_43165"
                     /product="hypothetical protein"
                     /protein_id="WP_263217321.1"
                     /transl_table=11
                     /translation="MSEPMEDLAPQPGRTGVGSRPTGPTALLKTVIAQASVIAALMFYL
                     GAMYSSQYYGYFHLSIYSLDFGFAPLVLQSLKLLRSPVLVAVVLVLIAATAPWYVMRRP
                     LSGQPAQRALAALAPVARVYPVVLAAGLVLLVFWSRIQPYEWAAPLTLAVGFLLGLVAH
                     AEGSRPGGAGRWGLLFFAAAVCLFWALTETALQLGEKDARVRASRVQSWPSVMLLSTRP
                     LSLPPPGVQEEKFARKDLRMPYRYTGLRLLIERGGRYYVVPVDWKARTDPVYVIHESDD
                     LWVGLGPGVQ"
ORIGIN
        1 gacttgaccg gcttgaccga gatcttctgc ggcgaggccg ccatgcgcgg caggatgaac
       61 gaccagaacc gggccacccg ttccctcgac agccactcgc cgtccacctc gccgagcgcc
      121 tcgaagccca cggtcgccgc gacgacggcc gccgccgcgt cctcgggcgc gacgtcggtc
      181 gccagctccc cggcgtcctg cgcctggacg acgagatcgt ggacccaggc gcaccaccac
      241 tgcggcaccc ccgccccgct gttgcgcgac gggtcggtgc ccagcttgaa gcccgccctc
      301 acgacggggt cggtggccac ggcggcgagc agccggcagg tggcgttcac gagtgactgc
      361 aacagggtgt cggccgagga cagacagcgt tcggccatct tctcgatgga gtccgccgcg
      421 gccccctcca cctccctggc cagcgcgtcc ttgctgggga agtggaagtg cagggcgccg
      481 gtgctcactc ccgcgcgctt gctgatcacg ggcagggtgg ccagcgcgta cccctcgtcg
      541 gcgaagacct cggccgccgc gaggatgagg gcctggcgcg tacgggccgc gcgcacctgt
      601 ttgaccatcg gtctcctcct ggggactgga gcggaacacg gagtgcgtca gtccgcttcc
      661 ctcaggggct tgcggcgttc cgtttcagga cattagcaaa ccgcatgatt ggtaggtttg
      721 ccagggtgga gaaacgcaac cctaatgcgg gcctgatggt cacacaccgt cagacaccct
      781 gctcgtattg tgtggagccg tcgatgatgg cgtatgcggg cggaccaagg ctcgcctcac
      841 aacgggctca caaaagagca tacgaggtct gctattgcgt cgccgtgagc ccgagggcac
      901 ggcccgcccg acccggtcga cccgtcgaac ggattccgca tcgccaccgg gaaccttcac
      961 gcgcgacgca gcaaaccaga tgcgaggttt gttgatatgc cgaaaccccc gcgaacacgt
     1021 ggggttcccc ggtccgcggg cgcgaaaacc cgcgctcggt caggcctcgg aagccgcgag
     1081 cgcggcctgc cggacacgct cggcttcctt gatgacccgg gcgcctctgt cgggcgccgt
     1141 gtccagccgg atcagtgccg cgggtgtggc gatggccggc agcatgtggt tgtacaggac
     1201 ggagacccgt tcgctgaggt ccgcccagtt ggagttcgcc tcggagaaca tctgcgtccc
     1261 cagccaggac ccgaggaagc attcggccgt ctcctggggg acgacgtggg gcagaacctc
     1321 gccacgctcc ttggcctcgg tcagcagctt ggtcgacagc ccgatccagg ccggccacgc
     1381 actgccgaac aggtcacgcc cctgcatgtc gccggagagg cgtatgcccg ccagcaggat
     1441 gacgtccagg gggatctgat gggccagcgt catgccgatg tccacccact cctggagctt
     1501 cagctcgcgc ggcacccagt agtcgttctc gatctgggct ccgatcacac cgcgcgccag
     1561 ggcctccttg gagtcgaagt ggaagtacag ggcgcccttc gtcactccgg cccgttcgag
     1621 gatctccgcg atggtcgccg ccgtgtaccc gcgttcggcg aagacggtgg ctgcggcctc
     1681 gataacagca cgacgggtcc gtaccgcacg ctcttgctgt gccacgggac cctcctccgt
     1741 tgaaaacaaa accgaacacg aggtacgtta ccccacttcg accgcgctta cccgggagtg
     1801 gacgggaagg aatccgatga tggccgggag tcgtcgggtg acgcgtgtga cgggcggggc
     1861 ccctggcgca gggcagccgc cgcccgtcac tcgcctcccc gtggtcacca ggcggtgcgg
     1921 cccccatcca ccggcagtgt cgcgccggtg acgaagctcg ccctgtcact gcacagccac
     1981 gcggcggtct ccgccacttc ctccggctcc gcggcccgcc cctgtggcgc ggaccgatag
     2041 gccttctcgg ccgccgcggg cgacacggcg aaccactcgt cgaccatctc cgagcgggtc
     2101 aggccggggg cgatggtgtt gacccggatc cggtgggccg cgtactccac ggccgcggcc
     2161 ttggtgagcc cgatcaccgc gtgcttggag gcgatgtagg gggcggccgt ggaggaggcg
     2221 accagcccgc ccacactgct ggtgttcacg atcgctcctc ccccgccctg caccatggcc
     2281 gtgatctcgt gacgcatgca gttccacacg ccgcgcacgt tcgtctccat gaccagttcg
     2341 tacgcctcgt cctccaggag gtgcagcggc accttctcca ccccgacgcc ggcgttgttg
     2401 aacgcggcgt ccaggcggcc gtagcgctcg atggtgtgcc gtacggcccg ctcgacctgt
     2461 tcgccgcggc gcacgtccat ggcgtgatag ctcgcctcgg cacccttgcc gcgcagttgg
     2521 gcgacaaggt ccctgagccg gtcctcgcgc cgggccgtca gcacgacaac ggctccctgc
     2581 cgcgcgaaca cccgccccgc cgcggctccg atgccactgc tggcgccggt gatgaggacg
     2641 accttgcccg ccagcggtgc ggtgcccgcc ccggtcatga ccgcgccccc ggcgcctcgg
     2701 cggccggctg cggcggggtc ggttcgccgt gccgccacac ggtgcgcccg gcggcctcgg
     2761 cgtcgagggc tcgcagcatc gcctcgtcga tcgcggccaa ctcccgcacc aggtaccgga
     2821 ctccggcccg ccgcatgagc tcttgctgga atccgtgcgc gaaggggctg gggtgtccga
     2881 tgaagggcac gtccaattcc ctggccttct cggcgacgga ggccgcgtcg tcgatgaaga
     2941 gcgcctggtg gggggcgagg gagaagaagt cgcgggtgat ctcgcggatg ccgggccgga
     3001 aggcgttggt gcagatgtag cccggctcgc tgaagtacgc ggcccacggc ccgagatgac
     3061 gctggaagtg actctcgtcc agtccgccgt agcacacgag gtccaggccg agcgcacgca
     3121 gccgctccag cagggcgacc gctccgtcga gcagccgtac gggctgctca cgaaggtagc
     3181 gctcgcgttc cgcgaagtag gcgccaagca cctccagcgg agtggcgctg gtgccggtga
     3241 cctccacgaa cacctgggcg gcctcgagtt gcggccggga gagcagggcc cgctcgagtt
     3301 caggcgtgta ctcgactccc tgggcggcga cgagccggtg catgaccggg ctgaaggtgt
     3361 cgttcagcag gacgccgtcg atgttgacgg cggccagccg gagcgtgctc agtgcggggt
     3421 gggacacggg gagacctccc tgtggtcgcg ggacgggcag cgctcatgaa cggggtgaca
     3481 gcagcaccgg aagttccgtg tggccgctgg atatgaagga ctcgaacagc cccagctcgc
     3541 gaggctcgac ggccagttcg aggtgcggga accgctcgaa gagggcgggc agcgcgatcg
     3601 tcgcctcgct catggccagc gggcggccca ggcagtggtg gacgccgtgg ccgaaggaga
     3661 agtgctcgtg gcgcgtgggg cgggtggcgt cgaaggtgtc cgcgtcgcgt ccgtgtgcgc
     3721 cggggtcgcg gccggcggcg gcgagcgcga gcaggatcgg ctcccccgcg cggatcacgg
     3781 tgccgtcgag gtcgaggtcc tcgacggcga accggagcag gatgttggcc accggtgcct
     3841 gggagcgcag cgtctcgtgg accaccgctt cccaggtgac ggtcccctcc cgcaccagcc
     3901 ggagctgctc gggatgggtg agcagggcgc agatggcgtg atccaggagg ttgacggtcg
     3961 tctcgtgccc cgccccgatg atcagcagga gggtgtccac gagttccttc tccgtcagtc
     4021 cctccccgtc ctcgtcgcgc atggtgatca gggcgctggt gagatcggtg cccgggtgga
     4081 cgcgcttgcg cccgacgagg tcgttcatca gcccgtacag ctcggcctgg ttcttctggg
     4141 ccgtctccgg agtcgtggtg gtggtgaaca gcacgtccgt gacccggtgg aggtcgtcgc
     4201 gctcacgggc gtccaggccg agcgtctcgg agatcacccg gatgggcagc tcgtaggcga
     4261 actccttgcg gatgtcgacg ggttgggccg acggcagccc ttccagccgg gtcagcagct
     4321 catcggtgat ctcggctatg cgtggtctga ggtccaccgt gcgccgggcg gtgaaggcgc
     4381 cggcgaccag cttgcgcagc ctggtgtgct cctcgccgta ggcggtgagc atgctgcgca
     4441 ccgacaccca catggccagc ggccacgagt cgtcgacctc gccgttgatc cacgcgggcc
     4501 agtgacggta ggcgtccttg gacacccgcg gctcggccag cacctgcttg atcaggtcgc
     4561 cccggttcac cgaccaggcg tgcacgcctc ccggcagggc gacttggacg acggcgccga
     4621 gtcgcctcag ccgggccgcc tcgccgtgaa ggtcctggcc ggaggggtcg agcaccacgg
     4681 gaacggacgg attctgcacc tgctgcctcc aaaacgacga tcgagcataa gggtaaagaa
     4741 aaccgctggt aaggtttttt tactgaccgc tcgcatagat caaggaacgg tcaggactgt
     4801 cgatttgttc ttcaatgcac cgaacgtcgc tttgatcgat cgaagttgat acaggatcaa
     4861 accgacagaa acggaatcta tggttcaagc gttcttcgga cacttgaaca atcctttacc
     4921 tggctggcga tagaagaaaa ccggtaggtc cgtatcttat tagctgtcgt cgaacaaagc
     4981 ttcaagacgg ggggtctccg atgggtctgc ctaccagcca gagcacagtc gaactgcccg
     5041 cactgcctcg actcaccacg acggtcccca gggaatacgt ccaccgcgcg agcctcgcgg
     5101 aggtcttcct cacgggctgc acggcccagg acgacctgag gttctccctc acgggtcagt
     5161 ggccccgggc gcacacactc ttcaccagcc ccgacgggag cagccacgat cctctgctca
     5221 ccgcagagac gtttcgccag gccggcatgt tcctcgccca cgcggagctc ggcgtgccgc
     5281 tccaccaccg cttcgtgctg ggcgacatca actacacgac ggccctgcag aacctgcgga
     5341 tcgcgctcag cccgacggac ttcgacctcg aggccaggtg cacggaggtc acctggcgcc
     5401 ggggcatggc cagttggctg cagctggagc tgtccatccg gcgccagggc aacgtggtgg
     5461 cacacggctc cggcagcttc tgcgtgatcc agcagggcgt ctacaagcgg ctgcgcgcgg
     5521 cccgcaagcc gtggtcgggc cccgccttcg acatccaggc ggaccgcgcc ccgctcccac
     5581 cggcgaccgt gggccgcggc tccactgccg atgtggtgct gacagccacc gactcgccca
     5641 ccagctggct gctcacgccg cgcctcgacc acccgatcct cttcgaccac gccgacgacc
     5701 atctgcccgg catggtcctg ctggagggcg cacggcaggc ggcctccgcc ctggtggcgc
     5761 cccggacgat gactccggcc agcgcgtcca gctcgttccg ccgctacgtc gaactcgacc
     5821 ggccctgctg gatcgacatc acgcgggtgt cccccgagag cggcggaatg atgaccgtgg
     5881 aggtcaccgg ccgtcaggac gacgagacgg tgttcacctc gacgatcacc ggaccggtcg
     5941 gatgagggtg acagccacgg catcgtcaca cccactcccc cgcctacgag acgtgcggcc
     6001 tgcgttgagt gacgtgcagc ggaccgaaag tccgctgcac gtgcacatta gcgtgcagcg
     6061 gtcccgtccc atagaggatt aaaaccggaa ggtctgttgt taacgcggca gtcgccaggg
     6121 cgagttgtac cgagacaggc aatcgccgac cgggctcgac cgcggccacg cgcacggacc
     6181 gacccccggc ggtacgtgcg cgtggccgac tccccgcacg ggagggcgta ccggatgcgc
     6241 cgcgacagcg gccgtcgtac ggaccccgcc cgcaccgcga ccgccgaccg aaccccaccc
     6301 cgcgaccgcc cacgacgcct attacggcgt aatagcccgg ccggggtacg actctgtcgg
     6361 caaacgccgc cctacagcgg cagcgtcagc ccgccctccc gtacctccgg tgaggtcggt
     6421 ccgccctccg aacgctccgt cttgcgcccc ttcttgcccg gtgccagcgt gcgcgggtcc
     6481 acggcctcgg ccggcgcccc ggtcgcgtac agcccgtcgg gcacggcgtc ccggtcgtgc
     6541 ggcagcagcc agaacacccg gcgccggaag gtgcctgagg accgcgacgg cttggcgctg
     6601 ggaccgagca gggcgtagcc gaccatacgt ccgtcgcggt ggtaggcggg cctgcccttg
     6661 cgggtgggca accggtccag actctggcgt acgtagtcca gttccgtgat gtcctcgagc
     6721 cagacgaagt cgacctcgtg gatgatctcg tcctctccta tgagggcgct catgctgtct
     6781 cctcgtcggc gaccagtccg atgcccgggt agtacttgcg ctggttggac aggatcatct
     6841 ccttcggaga cgccagcccc accacctctc ggacccgcgc cgcgaaggcc cgggacgata
     6901 tcgccggagc cccctcattt tgacaccacg tcttgtaggc cgcgtagagc tgggcctgtt
     6961 ccgctctcag atccggcccg atacagcact cctccgacag gaaccgcccg gtgtggtcct
     7021 cggtctccgc gtacgcggtg gtggcgatgc gcacccgctc cggaccggcg aggtccttct
     7081 cgcccctcag atagcggcgg gcgcccagga tcagccagtt gaggatgccc ggcccttcct
     7141 cggtgaccag catgtccgcc aggttgtcga tcttgcggtc gtcggacacg acccgctcga
     7201 acgggatcag ccgcatccgg cgccagaagg cgaagccgcc ggtgcccacc tcgggccggt
     7261 ggttgccgag cagccagagc ttgtgggtgg gctggaagct gaagaagtcc tgccgcatcc
     7321 gccgcgcctt gatccggtcg ccgccggtca gcagcttcac ccgcgcctcg tcgaaccggt
     7381 cccccggctt gacctcgctg cacacgatga cccgccgccc gtgcagctcg gccaggtcgg
     7441 tcggatgccc ctcgtagggc cgggccatca ggaacccggg cggcgcggcg tccgcgtagt
     7501 cgccgacgag cttcatcagc acgtccagga gcaccgactt gccgttcttg ccggagccga
     7561 acaggaacgg catcacctgg ccgccgacgt ccccggtgat ggagtagccc agcagcagat
     7621 gcaggaagtc gatcatttcc gcgccctcgg gcccgtcgcc gaaggtgtcg gtgaggaacc
     7681 ggtcccagcg cggggtcggc atcgcctgcg ggcccgcggt ggtggagcgg gagtggaagt
     7741 ccttgtccgg gtcgggcgta cggatcagac cggtgcgcag gtcgacgatg ccggccgggg
     7801 tgcacagcgc gtaggggtcg gcgtccagcc gagcggcgtt gagcaccatg cccggcgcgg
     7861 acctggcctg ggtgagcatc gcgttcatgc cactggtgct cagcgcgcgc cggcggtgct
     7921 gctgcagcgc cgaggcgctg tgggcgccgc gcgggtcgtc ggtcgccagg gactcggcca
     7981 ggtcccccgc ggcccacatc accgtgtcgt cctcgtcgat ctgccagcgg gtgctgtccc
     8041 agcggtacca gccgaggccg ggcacgtgcc ggtagtcgtt ggagtacagc ttgacgaaca
     8101 gtttggcgtt gccacggtcg ctgagggtgt ccggcagcag cccgtcagca gtggcctccc
     8161 cgtccccgcc cgtgccgtgc cggggctcga cggcctgccg gggcagctgg gcgccgggct
     8221 gggcgaggat ctgcgcggcg accgcctggg cgtcgaagag cgcgttgtcg tcgggggtca
     8281 cgcgcggcct cccggggaca acggacgctg ccggccggcg ctcagaccgg aacggatgat
     8341 ggcgcggtag cgccgctgct gccccggccg ggcatgctct gcggcctcgt ccagcacccg
     8401 ctcggcctcc tcctcggtca agtgcccggc ggcgaccagt cccccggcgg tgtaggcggc
     8461 ccggttgagt ttctccgaga agcccgcccc ttcggacacg gcggcacagg cggccacttc
     8521 ggccagtacg gcgctcaggg cccgccccgc ggccgcgtgc cctccccggc ggccagcacg
     8581 gcctgccggg cccgtggcgg caccgggcgc ggcacgggca cgtgcgcggg cggcagatgc
     8641 ccggtgcgct ccagctcgcg ggccagccag cccggcagcg gcgcgggctc ccgcaccggc
     8701 ccgacaggct cgtacacccc tgccgcggtc acggtgcccg gggcgacgat gtacccgccg
     8761 tgcgcccgta cgtcgacctg ccaggccagc gaacggcccc cgcccgaccc ggtggagcac
     8821 tgccagcgct gtccgccctg ggcccggtac cagacgtgca ggcccccgga gggcgtgcgc
     8881 acgcgcaggg tgttctcgtc gtccgcgggg ctttgctcgc cacgcagagc ggccagcaca
     8941 cccatcgtat ggaagccgtt ggcgagcccg gtcagatcga caccggcgga gatctcgatg
     9001 cccggcagca gccggtcccg cccgggcagt tcctgggcgt gcgcgtcgat gtccacgacg
     9061 accagactcg cgggcccgca ggcgattccc acgccgaaac gcgggttggc cccccaccac
     9121 tgctcgaccc tggcgtagtc gagggtggcg gcgtggaacc cgtggcacca gcgcccggcg
     9181 gccgggcagc cgcagtcccg gtgggtgtgc ccgggctcgc ggcaggcctc gcagttgccc
     9241 gcgggcgtct tgcgccccgg cgccagcggg tggacgggcc agccccggcc gacgcaccag
     9301 cgtgccgttg ccagcgatct ggcggcatcg cgcggctcgg gagacggctg ccttgagtcg
     9361 ctgcgcggaa cccgcagctc agcaggcatc cacatccccc atcagcgact gaagcgactc
     9421 aacgactgag acagattagc gaaccacgca gacagaaggg gacacaggga aaacaaaaaa
     9481 ccctgcgtgt gtcgccgagt cgctccagcg actgaacgca aatcctccag cgactcaagc
     9541 cacttcggtc gctgtctcag tcgctcaacg aagtcgcagg tcaaagggta cccacacccc
     9601 aaaacagcga ctgaagcgac tcaaggtccc tatatatgac gtacacacgc gcacacgcgc
     9661 acgcgcgtta gccttcatat acccggacct tcggtcgctt cagtcgctgt ttcacccatg
     9721 aatccccgcc tgaccaggcc ttttccttca gcgaccgaag gcgactcaag cgactctcag
     9781 tcgctcctct tcagtcgctg tccacgcccc caccacatga acccccgatt cgggcagcga
     9841 ctgaacgcac gcagcgactc aagcgaccct gcagcgaacc tggacccacc acccctcccc
     9901 caccaccggg accccgcacc caacctggtc ccacagtcgc ttcggtcgct tcagtagctg
     9961 cccccgcccg ggctctggtc ctgcttctgg tcctgcccag gctctggtca ctggacgccc
    10021 ggtccgagcc cgacccagag gtcgtcgctc tcgtggatga cgtagaccgg atcggtcctg
    10081 gccttccagt cgacgggaac gacgtagtag cggccgccgc gttcgatcag gagccgcagt
    10141 cccgtatagc ggtacggcat ccgcaggtcc ttgcgagcaa acttctcctc ctggaccccg
    10201 ggcgggggca ggctcagcgg ccgggtgctg aggagcatca cgctgggcca gctctgcaca
    10261 cgactggcgc ggaccctggc gtccttctcc ccgagttgca acgcggtctc ggtgagcgcc
    10321 cagaacaggc agacggcggc ggcgaagaag agcaggcccc aacggcccgc tccccctggc
    10381 cggctgccct cggcatgtgc gaccaggccc agcaggaatc cgaccgcgag cgtgagcggg
    10441 gcggcccatt cgtacggctg gatccgtgac cagaagacca gcagcaccag cccggccgcc
    10501 aggaccaccg ggtagacccg tgctaccgga gccagggcag cgagagcccg ttgagcaggc
    10561 tgcccggaca gcggacgccg catgacgtac cagggagcgg tcgccgcgat caggacgagg
    10621 acgacagcca cgagaaccgg agaccgcagc agcttcaggc tctggagcac cagcggggcg
    10681 aagccgaagt cgagggagta gatcgagaga tggaagtagc cgtagtactg actgctatac
    10741 atcgccccca gatagaacat cagcgcggcg atgacggatg cctgcgcgat gacggtcttg
    10801 aggagtgccg tggggcccgt cggccggctc ccgacacctg tccgtccggg ctggggcgcc
    10861 aggtcctcca tgggctcact cacgtgccct cagggggaga cacttcggga gcgatgggag
    10921 ccgcgggaga cggcggcgtg gcgga
//
