LOCUS       NZ_CP106798            10216 bp    DNA     linear   CON 01-NOV-2023
DEFINITION  Streptomyces sp. HUAS 15-9 chromosome, complete genome.
ACCESSION   NZ_CP106798
VERSION     NZ_CP106798.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. HUAS 15-9
  ORGANISM  Streptomyces sp. HUAS 15-9
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9258818)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 169.8x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 03:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,774
            CDSs (total)                      :: 8,679
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 504
            CDSs (without protein)            :: 504
            Pseudo Genes (ambiguous residues) :: 0 of 504
            Pseudo Genes (frameshifted)       :: 260 of 504
            Pseudo Genes (incomplete)         :: 328 of 504
            Pseudo Genes (internal stop)      :: 29 of 504
            Pseudo Genes (multiple problems)  :: 107 of 504
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:51:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7965729
            Orig. end                         :: 7975945
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106798.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="25"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    1046..1064
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.36"
                     /tool="antismash"
     gene            complement(1868..4090)
                     /locus_tag="N8I87_RS36285"
                     /old_locus_tag="N8I87_36285"
     CDS             complement(1868..4090)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="N8I87_RS36285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_36285"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_263215091.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.7e-38, bitscore:
                     128.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAQQTRGRRRAAPAGRPVPKGRGRTVRTPTVLQMEAVECGAAS
                     LAMVLGHHGRHVPLEELRIACGVSRDGSRASNLLKAARGYGLTAKGMQMDLAALAEVTA
                     PAILFWEFNHYVVYDGMGRRFGRRGVHINDPAKGRRFVPFEEFDGSFTGVVLVMEPGEG
                     FAKGGRRPGVLGAMPARLRGTAGTMPAAVLASLLLVAVGAAVPALSRTYIDMFLIGGQT
                     SLLGVLFASMGTCVLLTLVLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFFAQRS
                     PADLVQRLQSNDQVAETLARDLAAAGVDAIVVVLYAVLLYAYDPQLTLVGIGVALLNVV
                     AMRLVVRLRATRTAKLRADTARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHATTLE
                     EQQRLGAPSAWLGVVAPTLATLNSALILWIGGMRAVEGHISVGLLVAFQALVTRFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFQADPLYARPGGGDSTRRLHGHVELENISFGYSP
                     LDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYAPWEGVIRIDGQRLEDIPR
                     GALASSVSFVDQDVFLFEGSVRDNVALWDPSIPDDAVVEALRDAALYDVVVRRPGGIHS
                     KVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRRRGCACV
                     VIAHRLSTVRDSDEIVVLQHGTIVERGRHDELVARGGAYAALVRER"
     gene            complement(4087..4893)
                     /locus_tag="N8I87_RS36290"
                     /old_locus_tag="N8I87_36290"
     CDS             complement(4087..4893)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="N8I87_RS36290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_36290"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_263215092.1"
                     /transl_table=11
                     /translation="MQFRQQALAKLQSPEELDLPVRLARPQGWLALCVAVVVMAAASVW
                     AVTGSVASTVSAQAILTHGQGSYILQSPVAGQVTAVLARQGERLPAGSPVLKVRTSQGD
                     SVVRTVAAGRVTALAATIGQIIRTGANVAAVEKVAHSSDPLYATVYVPAENAASVPADA
                     TVDLTVSSVSAQRYGVLRGHVKSVDRTAQSAQSIAAFLGDSQLGEQFTGKGRPVAVLVR
                     LDRSPSTRSGYRWSSASGPPYRLDSMTLATASIRLADQRPVDWLLP"
     gene            complement(5001..5216)
                     /locus_tag="N8I87_RS36295"
                     /old_locus_tag="N8I87_36295"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="N8I87_RS36295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_36295"
                     /product="type A2 lantipeptide"
                     /protein_id="WP_263215093.1"
                     /sec_met_domain="strepbact (E-value: 9.9e-35, bitscore:
                     117.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEISDAELDNVSGGLSVNALNTVTGAVNGIAPVSGL
                     ADTAVGTVEGVTGLNTAPVAGLVAGL"
     misc_feature    complement(5484..5497)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.96"
                     /tool="antismash"
     gene            complement(5725..6138)
                     /locus_tag="N8I87_RS36300"
                     /old_locus_tag="N8I87_36300"
     CDS             complement(5725..6138)
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988891.1"
                     /locus_tag="N8I87_RS36300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_36300"
                     /product="PaaI family thioesterase"
                     /protein_id="WP_263215094.1"
                     /transl_table=11
                     /translation="MTMTTAEADKILSANFAPWVLELGLSVQALDERRAVLRLPWSDRL
                     SREGGGMSGQALMAAADTATVIAVSAARGGFVPMTTVQQSTSFQRAVAGSDVLIEAVLS
                     KLGRRMAFADITMTDEGSGQIAARASTVYALLD"
     misc_feature    6129..6149
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.43"
                     /tool="antismash"
     misc_feature    6218..6229
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            6339..7802
                     /locus_tag="N8I87_RS36305"
                     /old_locus_tag="N8I87_36305"
     CDS             6339..7802
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057832.1"
                     /locus_tag="N8I87_RS36305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_36305"
                     /product="S1 family peptidase"
                     /protein_id="WP_263215095.1"
                     /transl_table=11
                     /translation="MPKRKAAIAVGGVAALGAAALLLPNAMASQDGGSADAAGAPRTMK
                     ANDASDLAAQLQKALGDAFAGSYYDSGKQQLVVNVVSGDNNLIVQAKKAGAVVRQVENS
                     TSELKAAAQTLKTKATIPGTSWAVDPRTNKVLVTADSTVTGAKWNRLESTVQGLGSGMA
                     TIKKSAGTFKTFVSGGDAIFSQVQGGNVRCSLGFNVKASDGSPAFLTAGHCGVAAKDWS
                     DSQNGQPIATVDQATFPGQGDFSLVKYNDATTQAPSEVNVGNGQAPVQIKQAVDATVGT
                     TVFRMGSTTGLHDGQVTGLDATVNFQSETDPNGVDTVTGLIQTNVCAEAGDSGGSLFTQ
                     DGGAVGLTSGGSGDCTSGGETFFQPVTAALQATGATLGDAGAGAGAGAGDQSGAGGDAV
                     GGGDQSGATADPSATADPSATADPSATATNPAGTGDQSGAGAGDQSGAGAGDQSGAGAG
                     DQSGTGDATGTGHQPGTGAGDGSAVNQGH"
     gene            complement(7960..10101)
                     /locus_tag="N8I87_RS36310"
                     /old_locus_tag="N8I87_36310"
     CDS             complement(7960..10101)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994900.1"
                     /locus_tag="N8I87_RS36310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_36310"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_263215096.1"
                     /transl_table=11
                     /translation="MSDGQKPAQAPEATGTRVGRPLLSLSLATMMDEVHAHSGAVYLLA
                     NDEPVLEMAVVAGLPRAFAAPWERVGLSAPIPVAEAVRGRRLVWVGGEDDMARRYPRIA
                     VVLPYPFALAALPVATAAKAYGAVFVTWPGSHPPELSDREREHLTAACDRLAVRLERAA
                     QDNRPIPREPDLLASTVTGVAGTLGTVEAARMVARLPYGLVSLDLHGQVGFANAAAAEL
                     LDTPAGELLGTHLWASVPWLNDPMYEDRYRAALISQHVTSFVALRPPGDWLSFRLYPST
                     TGLSVRISRARAVAEMGRAAPQTGAGRSRLVTISQVLSLAGALTEAVGVQDVVQLVADE
                     IVPSVGSQAVVLLGSQGGRLHVLGHRGYANPHHVERFDGIALSERMPGTHVLTTGVPAF
                     FDTRQQLDRLYPGLTGLPGGFAAWAYLPLIASGRPVGTCVLAYTEPHVFPADERAVLTS
                     LGGLIAQALARALLYDAKNQLAHGLQEALLPRSLMPPPGIEAAARYLPATQGMEIGGDF
                     YDVVPTRPFAAAVIGDVQGHNVTAAGLMGQIRTGVRAFATGGQAPQEVMASTNRLLVDL
                     GADLFASCLYLRLDPVNERAVMARAGHPPPLLRRPDGRVRVLDLAGGPLLGIDAEATYP
                     TTEVDLATGSVLVLYTDGLVESPGVDIEDALADMGDLLNTIGERPLEQLADELVAHSVA
                     ARERADDVAVLLLRALGPA"
     misc_feature    complement(10133..10148)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 23.65"
                     /tool="antismash"
ORIGIN
        1 gttcttgata cggcccacct tctgctggag ctcacggctg cgcgcgaact cgcccgccca
       61 ggcggcgtac gcatagttct cggccgccgc cacccgcagc ttcggcagcc cgcgcagcgt
      121 ctggaaggcc tggttgttca gcttgttgcc gagcaccacg agccgtcgct gccaacggac
      181 ctgccacagc ccgagcccca ggaacacggc ggcgatgacc gtgagcatgc cgatcgcggc
      241 gagcgccatg ggcacgctgt accagaacag cagggccagg ttcatcgcgc cgacggtcac
      301 cgactgggcg accaccgggc cgactcccgc aagcagtcgc cgtatcgcgc tgatccccat
      361 ggccgcgctc gccagctcgc ccgtggaccg ctgggtgaag aacttcgtcg gcagacgcaa
      421 cagccggtcc cataccgccg gttggagcgt cgcctcgata cggccctcca gacgcaggat
      481 ggtcaggttc tccagcagca tgaacgcggc cgccaccgcg ccgctgacca tcacggccag
      541 acaggcctgc acgatcagcc cctgctgagc cttcggtacg tactcgccga gcaccttgcc
      601 ggtggcgatc ggcaccagcg caccgatcgc caccgtcacc agaccggcga gcagcagacg
      661 cagcacgtca ccgcgcgtgc cccgcatgct gaaccgcagc agcccgagcg gaccgagcct
      721 ccgctcgggc agcgggcggt agaacatcac cgcgcgcggt tcgaactcct ccgcgttggc
      781 cttctcgaca ggggtctcgc gaccggtcgc cggatggacg gccacatagc cgccccgccg
      841 ccacagcagc gcgaccggcg ccccggacag ggcccggtgc ccgaccagcg ggccgatgtc
      901 gtcccgccac cagcggccgt ccaggcgtac ggccctggtg cgcacccggg atgcgagcgc
      961 cacccgctcg accgggtcga gccggtcgct ctcggtgccg ctctgcgcgg gttcggcgag
     1021 cgtgatcccg gcggcccggg cgaccagctt gcagacggtg tacgtggcgt cggcgtcggc
     1081 ggccgtcgtg cgtctcccag gcgccttgcc gatcgacgcg agcagcgtgc ggtcggcctg
     1141 ggcgcggacc gcctcacccg ccttgatccc ggccgccgtg cgcgtctcgt gggtgtgctc
     1201 cagctgctcg atccaccggt ccagggtggt gagcagccgg tactgctggt cgaccatgct
     1261 ctgccacacg gccgggtcca tcagcaggtc ggctgcggcc tccgcaccgt acagcgaccc
     1321 gtactggacg ctgcccggcg gcacctgcat ccagaacacg tcgtcgtccc ccacgctcga
     1381 atgaactcgc gcgggggcgc ccccatcgtc ggccgcgcgt tcggtcgtca tcggtgcctg
     1441 gaagaggatg gacaggccgc ggccgacgcc cagcgcgagg gcgtactcca gcgggctcga
     1501 cgtcgggggc acgtactggg ggttgccgta ctcgtcgtac gaccaggtct gggtgggcgc
     1561 gggctggtac agctcgcgca gcccgatgcg gtgcaccacg cagtcgcgca acgggcgtgc
     1621 gaccagggtg tgctgcggac cggcgaccgg gccgagcagc agcgagcccg cctccagacg
     1681 gcccaggtgg tgccagtggc cctgctgtcc ggcgtccacc gcgaacaggt ccagggcgcc
     1741 cgccgcgacc agccacagca cctgcgggcc ctcgaggtcg aggcggttga agccgctgcg
     1801 gtcgaggggc gtgcccagcg agccgagcgc gcccaggacg aggtcgcctt cgtgcacgga
     1861 ggtcatctca ccgctccctg accagcgcgg catatgctcc gccgcgcgcc accagctcgt
     1921 cgtgccggcc gcgttccacg atcgtgccgt gctggaggac gacgatctcg tcgctgtcgc
     1981 gcacggtgct cagccggtgg gcgatcacca cacaggcaca gccgcgccgg cgcagattgt
     2041 ccatgacgac cagctcggtc tccgcgtcca gcgcgctggt cacctcgtcc agcaccagga
     2101 tgctgggccg gcggaccaac gcccgtgcga tctccagacg ttgacgctga ccgccggaga
     2161 agttccggcc gtcctgctcg accttgctgt ggataccgcc gggccgccgc acgacgacgt
     2221 cgtacagggc cgcgtcccgc agcgcctcca ccaccgcgtc gtccgggatc gacgggtccc
     2281 acagcgccac gttgtcgcgg accgagccct cgaagaggaa cacgtcctgg tcgacgaagg
     2341 acaccgagga cgcgagcgcg ccccgcggaa tgtcctccag ccgctgcccg tcgatgcgga
     2401 tcaccccctc ccagggcgcg tacaggcccg agatcagccg ggagaccgtc gacttgccgc
     2461 tgcccgagcc gccgaccagc gccacctgct gcccgggtcc gacggtcagg tcgaagccgg
     2521 tgagcagggg cttgtccagg gggctgtagc cgaaggagat gttctccagc tcgacatggc
     2581 cgtgcagccg acgcgtggag tcaccgccgc cggggcgggc gtagagcggg tccgcctgga
     2641 agttctccac gtccttcaga cgggccacgt cggccgcgaa gtcctggatg cggcccgcca
     2701 cgccgttcag ccgggtgagc ggcgccgtga agcgggtgac aagtgcctgg aaggcgacca
     2761 gcagaccgac cgagatatgg ccctccaccg cccgcatacc gccgatccag aggatcagcg
     2821 cgctgttcag ggtggccagc gtgggcgcga ccacgccgag ccaggcgctg ggcgccccga
     2881 gccgctgctg ctcctccagg gtggtggcgt gctgtcccgc ccacttgcgg aaatagccgt
     2941 cctcgccgcc tgtcgccttc atcgtctcga tcaactggag cccggtgtag gccgtgttgg
     3001 tgagccgtgc ggtgtccgcc cgcagcttcg ccgtacgggt cgcgcgcagc cgtacgacga
     3061 ggcgcatcgc cacgacgttg agcagcgcca caccgatgcc gacgagagtg agctgcgggt
     3121 cgtaggcgta gagcagcacc gcgtagagca cgaccacgat cgcgtccaca cccgccgccg
     3181 cgaggtcacg ggccagggtc tcggccacct ggtcgttgga ctggagccgc tgcaccaggt
     3241 cggcggggct gcgctgggcg aagaacgtca ccggcagccg cagcagatgc cgcaggaagc
     3301 gggcgctgga gagggtggag gagatgatgc ggccgtgcag caggttggcc tgctggagcc
     3361 aggtcagcac cagcgtgagc agcacgcagg tgcccatcga cgcgaacagc acacccagca
     3421 gcgaggtctg gccgccgatc aggaacatgt cgatataggt acggctgagc gccggcaccg
     3481 ccgcgccgac cgcgaccagc agcaggctcg ccagcaccgc ggcgggcatg gtgcccgcag
     3541 tgccgcgcag ccgggccggc atcgcgccga ggacgccagg cctgcgcccg cccttcgcga
     3601 agccttcccc cggctccatg accaggacga ccccggtgaa gctcccgtcg aactcctcga
     3661 agggcacgaa acggcggccc ttggcggggt cgttgatatg gacgccgcgc cgtccgaagc
     3721 ggcgccccat gccgtcgtag acgacgtagt ggttgaactc ccagaagagg atggccggtg
     3781 ccgtcacctc cgcgagggcg gccaggtcca tctgcatgcc cttggcggtc aggccgtaac
     3841 cgcgcgccgc cttcagcaga ttgctggcgc gcgagccgtc acgggagaca ccgcaggcga
     3901 tgcgcagctc ctccagcggg acatggcggc cgtggtgccc gagcaccatg gcgagggagg
     3961 cggcaccgca ctccaccgcc tccatctgga gcacggtggg cgtacggacg gtccgcccgc
     4021 ggcccttggg gaccgggcgc ccggccggtg cggcgcgccg gcggccgcgg gtctgctgag
     4081 cggtcgtcac gggagcagcc aatcgacggg tcgctggtcg gccagccgga tcgaggccgt
     4141 ggccagggtc atggagtcga gccggtacgg cgggccgctc gcggacgacc accggtagcc
     4201 gctcctcgtc gagggcgacc ggtccagcct caccaggacg gccaccgggc ggcccttccc
     4261 ggtgaactgc tcgcccagct ggctgtcccc gaggaacgcg gcgatcgact gggcggactg
     4321 ggcggtgcgg tccaccgact tcacatggcc gcgcagcacg ccgtaccgct gcgcggacac
     4381 cgaggacacg gtgaggtcga ccgtggcgtc ggcggggacg gaggccgcgt tctcggcggg
     4441 gacgtacacc gtcgcgtaca gcgggtccga ggaatgggcg accttctcca ccgcggccac
     4501 gttcgcgccg gtcctgatga tctgcccgat ggtggcggcg agtgcggtga cgcggcccgc
     4561 ggcgaccgtg cggacgaccg agtcgccctg gctcgtacgg accttcagga cgggcgagcc
     4621 tgcgggcagc cgctcaccct gccgggcgag gacggcggtg acctggccgg cgacggggct
     4681 ctgcaggatg taactgccct gcccgtgcgt gaggatggcc tgcgcgctca ccgtggaggc
     4741 gaccgagccg gtcaccgccc agacggaggc ggccgccatc acgacgacgg cgacgcagag
     4801 cgccagccag ccctgcgggc gggcgagacg caccggaagg tcgagctcct ccggcgactg
     4861 gagcttggcg agggcctgtt gacggaactg cacggaactt ccctcacctg tgggagaggc
     4921 tgcgccacga catggttctg ggcatccgag agtcccggag ccgggatacg gctccgggac
     4981 tcaacggcag cgaggtgcga tcagagaccg gcgaccaggc cggcgaccgg ggcggtgttc
     5041 agaccggtga caccctcgac ggtgccgacg gccgtgtccg ccaggccgga aaccggggca
     5101 atgccgttca cggcaccggt gacggtgttg agggcgttca cggaaaggcc gccggagacg
     5161 ttgtccagct cggcgtccga gatctcgacg gtctcgacct ggggggtgga gttcatgatg
     5221 gaacttccct tcatacgaat atttcacaag ggggagagcg gccccctccg agaacagatg
     5281 gacggccgcg accgcgggcg ccgggcaccc tttcgggaag cccttcgcgg ctcccgcgac
     5341 gcgatggatc aaagcacgca cgcggctcgc gcagccaatc aaccaggcgt ctcacctggg
     5401 aacttgacct tcgcagggac ccatcggtga gggcgcgcgc acggcttgtc ggcgactcct
     5461 tcacatggcg cacggcatcg gcgcagacaa acgcttgccc cggggcgggc aatccggcac
     5521 tccgcgccga aggcgcgagg aggggcacgc gattgtgcag aaccgccgga cccggtgact
     5581 tggttgagta ttcaatgtgc agattccctg aagcagggat tccggtccgg gtccggaacg
     5641 gaccgtgtcc ggacggttgc cgaccgtgtc gattcggagg tcccagaggt cgccgaccgt
     5701 gtcagcgcgg cagcgcaggc cgtgtcagtc cagcagtgcg tagaccgtgc tcgcccgcgc
     5761 ggcgatctgc ccggagccct cgtcggtcat cgtgatgtcc gcgaacgcca tccgccggcc
     5821 cagcttgctc agcaccgctt cgatcaggac atccgaaccg gccaccgccc gctggaacga
     5881 cgtcgactgc tgcaccgtcg tcatcgggac gaagccaccg cgcgcggccg acacggcgat
     5941 caccgtcgcg gtgtcggcgg cggccatcag ggcctgtccc gacatccctc cgccctcccg
     6001 ggaaagccga tccgaccagg gcagacgcag caccgcacgc ctctcgtcca atgcttggac
     6061 cgacagcccg agttcaagga cccacggggc gaagttggcg gagaggatct tgtcggcttc
     6121 ggcggtcgtc atcgtcatat gggcgattgt tcccgaccgt gccccgcgac acgcgcggcg
     6181 tggccgattc accgtcacgc aaagcaagtt ggcaaacgga attgaacgcc gcgctcgacc
     6241 cgtccgtacc tacagccatc caggcgcccc ccgatcaact gccggacatc ggcagagacc
     6301 ccgtcccttg gaggtcgaga agtttgagtc acaagcgaat gccgaagcgc aaggccgcga
     6361 tagcggtggg cggtgtcgcg gcgctcggag cggcggccct tctgctgccg aacgcgatgg
     6421 cgtcccagga cggcggctcg gcggatgccg ccggcgctcc caggaccatg aaggccaatg
     6481 acgcctcgga cctggccgcg cagctccaga aggcgctcgg tgacgccttc gccggctcct
     6541 actacgactc cggcaagcag cagctcgtcg tcaacgtggt cagcggcgac aacaatctca
     6601 tcgtccaggc caagaaggcg ggcgcggtcg tccgccaggt ggagaacagc acctccgaac
     6661 tcaaggccgc cgcccagacc ctgaagacca aggcgaccat cccgggcacc tcctgggccg
     6721 tcgaccccag gaccaacaag gtcctggtca ccgccgacag caccgtcacg ggcgcaaagt
     6781 ggaacagact ggagtcgacg gtccagggcc tcggctcggg catggcgacc atcaagaagt
     6841 ccgccggcac gttcaagacc ttcgtgtcgg gcggcgacgc catcttctcc caggtgcagg
     6901 gcggcaacgt ccgctgctcc ctcggcttca acgtcaaggc gtccgacggc agccccgcct
     6961 tcctgacggc cggtcactgc ggtgtcgcgg ccaaggactg gtccgactcc cagaacgggc
     7021 agcccatcgc caccgtggac caggccacct tccccggcca gggcgacttc tcgctcgtca
     7081 agtacaacga cgccaccacc caggcgccga gcgaggtcaa cgtcggcaac ggccaggcac
     7141 ccgtccagat caagcaggcc gtggacgcca ccgtcggcac gacggtcttc cggatgggct
     7201 ccaccaccgg tctgcacgac ggccaggtga ccggcctcga cgccaccgtc aacttccaga
     7261 gcgagaccga ccccaacggc gtcgacacgg tcaccggact catccagacc aacgtctgcg
     7321 ccgaggccgg cgacagcggc ggctccctgt tcacccagga cggcggcgcg gtcggcctga
     7381 cctccggtgg cagcggcgac tgcaccagcg gcggcgagac cttcttccag ccggtgaccg
     7441 ccgccctgca ggcgaccggc gcgacgctcg gcgacgccgg tgcgggtgcc ggtgcgggtg
     7501 ccggggacca gtccggtgcc ggcggtgacg cggtgggcgg cggcgaccag tccggcgcca
     7561 cggccgaccc gtccgccacg gctgacccgt ccgccacggc cgacccgtcc gcgacggcga
     7621 ccaacccggc gggtacgggc gaccagtccg gtgcgggtgc gggcgaccag tccggtgcgg
     7681 gtgcgggcga ccagtccggt gcgggtgcgg gcgaccagtc cggcaccggt gacgcgaccg
     7741 gtacgggcca ccagccgggt accggcgcgg gcgacggctc ggccgtcaac cagggccact
     7801 gaaccagggc cggtcgagcc tggaccggct gaagacgcag atcggctgaa cccagaccca
     7861 ctgatcggcc tcaccagcgg ttccggccgg cggtggacgg tccggctctc cggcgggagg
     7921 gccggaccgc gtcgtcgtac gaggcgggtc cgccgcggct caggcgggcc ccagcgcccg
     7981 cagcagcagc accgccacgt cgtcggcccg ctcccgtgcc gccacgctgt gcgcgacgag
     8041 ttcgtcggcc agttgctcca gcggccgttc cccgatcgtg ttcagcaggt cacccatgtc
     8101 ggccagggcg tcctcgatgt ccacgccggg cgattcgacg aggccgtcgg tgtacaggac
     8161 gaggaccgag cccgtggcga ggtcgacctc cgtcgtcggg tacgtcgcct ccgcgtcgat
     8221 accgagcagc ggccccccgg ccaggtccag gacgcgcacc cgcccgtccg gccgtctcag
     8281 cagcggtggc ggatgccccg cacgggccat cacggcacgc tcgttgaccg gatcgagccg
     8341 cagatacaga cagctcgcga acagatcggc gccgaggtcg acgagcagcc ggttggtgct
     8401 ggccatcacc tcctgcggcg cctggccgcc ggtggcgaac gcgcgtaccc cggtgcggat
     8461 ctgccccatc agcccggccg ccgtcacgtt gtgtccctgg acgtccccga tcaccgccgc
     8521 cgcgaacggc cgggtcggca ccacgtcgta gaagtcgccg ccgatctcca tgccctgagt
     8581 ggccggcaga tagcgcgcgg ccgcctcgat gcccggcggc ggcatcaagg agcgcggcag
     8641 cagggcctcc tgcagaccgt gcgccaactg gttcttggcg tcgtacagca gtgcccgcgc
     8701 cagggcctgc gcgatcagcc cgcccaggct tgtcagaacg gcccgctcgt ccgctgggaa
     8761 gacatgcggc tccgtgtagg cgagcacaca ggtccccacc ggccgccctg acgcgatcag
     8821 cggcagatag gcccaggccg cgaagccgcc cggcagcccg gtcagccccg gatacaggcg
     8881 gtccagctgc tgccgggtgt cgaagaacgc cggcaccccc gtggtcagga cgtgggtgcc
     8941 cggcatccgc tcggacagcg caataccgtc gaaccgctcg acatggtgcg ggttcgcata
     9001 cccgcggtgc cccagcacgt gcagccgccc gccctgcgac ccgagcagca ccacggcctg
     9061 actacccacg gacgggacga tctcgtccgc gaccagctgc accacgtcct gcacgcccac
     9121 cgcctcggtc agcgctccgg ccaggctcag cacctgggag atggtgacca gacgggaccg
     9181 tcccgcaccg gtctgcggag cggcccgccc catctccgcc accgcccgtg ccctgctgat
     9241 gcgcacgctc agcccggtcg tgctcgggta gagccggaac gacagccagt cgcccggcgg
     9301 gcgcagcgcc acgaacgacg tcacgtgctg gctgatcagg gcggcccggt accggtcctc
     9361 gtacatcggg tcgttcagcc agggcaccga ggcccacaga tgggtgccga ggagctcacc
     9421 ggcgggtgtg tcgagcagct cggccgccgc cgcgttggcg aacccgacct ggccgtgcag
     9481 atcgagcgac accagcccgt acggcagccg tgccaccatc cgcgcggcct cgaccgtgcc
     9541 cagggtgccc gcgacccccg tcacggtcga cgccagcaga tcgggctcgc gcgggatcgg
     9601 ccggttgtcc tgcgccgccc gctccaggcg caccgcgagc cggtcacagg ccgcggtcag
     9661 atgctcacgc tcccggtcgg acagttccgg cggatgcgaa ccgggccagg tcacgaagac
     9721 cgcgccgtac gccttggccg cggtggccac gggcagggcg gccagcgcga agggataggg
     9781 caggaccacc gcgatacggg ggtagcgccg ggccatgtcg tcctcgccgc ccacccagac
     9841 cagccgccgg ccgcggaccg cctccgcgac cgggatcggc gcgctcagcc ccacccgctc
     9901 ccacggcgcc gcgaacgccc ggggcagccc agccaccacc gccatctcca gtaccggctc
     9961 gtcgttcgcc agcaggtaca ccgccccgga gtgggcgtgg acctcgtcca tcatcgtggc
    10021 cagcgacaag gagagcagcg gtcggccgac ccgcgtaccg gtggcctccg gcgcctgcgc
    10081 gggcttctgc ccgtcggaca cgccgaccac ctcctcgtac ggccgcgcgt ctcacccccc
    10141 aggggtcaag gctctctcct ggcggcgcgc ggcgcacggc gagcgggccg cgccggaccc
    10201 ccgtgagctc cacgga
//
