LOCUS       NZ_CP106798            11878 bp    DNA     linear   CON 01-NOV-2023
DEFINITION  Streptomyces sp. HUAS 15-9 chromosome, complete genome.
ACCESSION   NZ_CP106798
VERSION     NZ_CP106798.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. HUAS 15-9
  ORGANISM  Streptomyces sp. HUAS 15-9
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9258818)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 169.8x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 03:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,774
            CDSs (total)                      :: 8,679
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 504
            CDSs (without protein)            :: 504
            Pseudo Genes (ambiguous residues) :: 0 of 504
            Pseudo Genes (frameshifted)       :: 260 of 504
            Pseudo Genes (incomplete)         :: 328 of 504
            Pseudo Genes (internal stop)      :: 29 of 504
            Pseudo Genes (multiple problems)  :: 107 of 504
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:51:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6798721
            Orig. end                         :: 6810599
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106798.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11878
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11878
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11878
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6878](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6878
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            800..2269
                     /locus_tag="N8I87_RS31095"
                     /old_locus_tag="N8I87_31095"
     CDS             800..2269
                     /GO_function="GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033815.1"
                     /locus_tag="N8I87_RS31095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_31095"
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="WP_263213685.1"
                     /transl_table=11
                     /translation="MFLTIGTTGTPERPATDLGFLLHKHPEKAQVFSTSYGTAHVLYPE
                     ADAERCTAALLLEVDAVALVRRGKGKGRGGAPDAALAQYVNDRPYAASSLLAVALSSVF
                     SSAMRGVCAARPERATQPLPLRVEVPALPARGGPELVRRLFEPLGWTVSVEPVALDTAF
                     PQWGDSRYVRLVLESEALTLAEALRHLYVLLPVLDDAKHYWVASDEVDKLLRAGEGWLP
                     GHPEQKLITSRYLSRRWSLTRQAMERLELVRLAEADDSDVEEIDNAVEEDSEAEEKPTP
                     LAVQRRDAIIAALGESGAARVLDLGCGQGQLVQALLKDPRFTEIVGVDVSMRALTIAGR
                     RLKLDRMGERQASRVRLQQGSLAYTDSRLKGYDAAVLSEVIEHLDLPRLPALEYAVFGA
                     ARPRTVLVTTPNVEYNVRWESLPAGHVRHGDHRFEWTREEFRTWAGVVAERHGYDVEFR
                     PVGPDDPEVGPPTQMAAFSIRTTNEKEAKAA"
     gene            2266..4806
                     /locus_tag="N8I87_RS31100"
                     /old_locus_tag="N8I87_31100"
     CDS             2266..4806
                     /GO_function="GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /GO_function="GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA]"
                     /GO_process="GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122488.1"
                     /locus_tag="N8I87_RS31100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_31100"
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="WP_263213687.1"
                     /transl_table=11
                     /translation="MTETRKARVLPVTDLSLVVLVGASGSGKSTFARRHFKPTEIISSD
                     FCRGLVSDDENDQSATTDAFDVLHYIAGKRLAAGRRTVVDATSVQQDSRRQLIELARKY
                     DVLPIAIVLDTPEEVCAERNAARTDRADMPRRVIQRHIRELRRSIRHLEREGFRKVHIL
                     RGVEEADGATVVTERRFNDLTHLTGPFDVIGDIHGCASELESLLGKLGYSDGVHPEGRT
                     AVFVGDLVDRGPDTPGVLRRVMSMVASGNALCVPGNHENKYGRYLKGRKVQHTHGLAET
                     IEQMEGESEEFTAQVKEFLDGLVSHYVLDGGRLVVCHAGLPERYHGRTSGRVRSHALYG
                     DTTGETDEFGLPVRYPWAEDYRGRAAVVYGHTPVPEATWLNNTICLDTGAVFGGKLTAL
                     RWPERQLVDVPAERVWYEPAKPLRSEAPGGHDGRPLDLADVHGRRVVETRHAGRVAVRE
                     ENAAAALEVMSRFAIDPRLLPYLPPTMAPTATSHMEGYLEHPAEAFAQYAQDGVARVVC
                     EEKHMGSRAVALVCRDAEAARKKFGVDGPTGSLYTRTGRPFFDDEAVTEEILGRVRSAI
                     GEAGLWDELDTDWLLLDAELMPWSLKASGLLRSQYAAVGAASGAVFPGALAALEGAAAR
                     GVDVTGLLSRQRERAADAAAFTDAYRRYCWTTDGLDGVRLAPFQILAVQDRSLAALPHD
                     EQLALIDRLVEHDGTGLLQNTRRLYVDTGDPESVRAGVDWWLEMTGRGGEGMVVKPVGA
                     VVRSDEGRLVQPGIKCRGREYLRIIYGPEYTRPDNLDRLRKRFLNHKRSLAIREYALGL
                     EALDRLAEGEPLWRVHEAVFGVLALESEPVDPRL"
     misc_feature    4866..4881
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.79"
                     /tool="antismash"
     misc_feature    complement(4867..4880)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.06"
                     /tool="antismash"
     gene            5001..6878
                     /locus_tag="N8I87_RS31105"
                     /old_locus_tag="N8I87_31105"
     CDS             5001..6878
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675582.1"
                     /locus_tag="N8I87_RS31105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_31105"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_263213689.1"
                     /sec_met_domain="TIGR03975 (E-value: 5.3e-167, bitscore:
                     555.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVHLVTMPWHPIDLPSLQVGLLHRLVRRARPADEVTEFHGSLRW
                     AEFLLEHSGGRLRPGDYVAVGSDSIFHGLGDWVFSGVLYDDPDWGSARLRAYAADRDVF
                     IDTALEMRPYAAGFIDECAAEVLAAEPDVVGFTTTFMQNVSSLALARELKRRRPELTVV
                     FGGSNCDGPMGHALHRNHRFVDHVVRGEGEYALPALLRHVDAGTPPVDVPGLCWWDGDM
                     SRANTETRRTVAPADIPSPDYDQWQAALEPSPVLEYVHPKLVVEGARGCWWGEKHHCTF
                     CGLNGSSMAFRAKPGERLWEEIDRLVRRHRILDIVTVDNIIDMAYFRDFLPRVVDSGWD
                     LRLHYEVKSNLTPDQLRLLGRSGTVHIQPGIESLGSRVLDLMDKGVSGARNIRTLRECE
                     NHSLTCSWNLLYGFPGESAEDYAHVVDQLPALVHLQPPSGAHRIQLERFSPHFTDRALG
                     FGKRRPAAMYQHVYDLPEDELADLVYLFDTEDAGIGGETEHRLKAAVAQWRAGHHTSRL
                     LFEETEGDEGGGADALLVHDRRHGRPSATHRFTGWAAAALRRLEDGRTESALHRLLTAD
                     GHSVSPEALREWLQHTFALGLLFRDGRTYVSLPTWDETVRVPDGAGVAG"
     misc_feature    5586..5588
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6875..7417
                     /locus_tag="N8I87_RS31110"
                     /old_locus_tag="N8I87_31110"
     CDS             6875..7417
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019061125.1"
                     /locus_tag="N8I87_RS31110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_31110"
                     /product="DUF5825 family protein"
                     /protein_id="WP_263213690.1"
                     /transl_table=11
                     /translation="MTGLPTAVPHRVTGRRVEVTEPLRLGAGGQRTAVAVQFLRECQSL
                     GLRVGWTAADRETPYDIGLLRHLSPPAQVSDEPGELREWRAGYAYGMLYHRRGPDFVAV
                     MDRREPGTAVRLTLDHPDLLSAFHTLLNPTPLDELDPGPREAAGLLAAERLALVTDGWA
                     VALPPRIGRWPVPCTGI"
     gene            7421..8704
                     /locus_tag="N8I87_RS31115"
                     /old_locus_tag="N8I87_31115"
     CDS             7421..8704
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019061126.1"
                     /locus_tag="N8I87_RS31115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_31115"
                     /product="MFS transporter"
                     /protein_id="WP_263213692.1"
                     /transl_table=11
                     /translation="MAAATAEARPLPLRRQRAFVLLCGEQIAGSVGRQVTALAVALLAV
                     TDLGTGPFGAAAVLALTYLPGALLSPFAGVLVDRVRLRRLLVVVTAAQALVVGSVPLVN
                     TLGGVGRPQLYGVAVVSGALTFTLSVALQTALPRVVAPDRLLSANSALTGARTAGLIGG
                     PALGGVLVGILGPAPALLAGSAAYAVEALLLLALPAALNDPAEPRGAQSPASLLRTGLE
                     VLRGEPRLRLMVLAGAGLNLGSGASGALLVFYATRDLALSAWQLGISYAVHSAATLAGV
                     GVAGRFARTAGLWSATRICALLAGGAVFLIPAASLGPAFELLLGYQACYGLAVTVWAIS
                     MTTLQQLVTPDGMRGRVAGFVQAALLGTVPVGALAGGALAAGIGNVPVLLGSAVVALLA
                     AASLWTPGVSRPAAVAARTPEPGSSAPR"
     gene            complement(8643..9542)
                     /locus_tag="N8I87_RS31120"
                     /old_locus_tag="N8I87_31120"
     CDS             complement(8643..9542)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385308.1"
                     /locus_tag="N8I87_RS31120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_31120"
                     /product="hypothetical protein"
                     /protein_id="WP_263213694.1"
                     /transl_table=11
                     /translation="MSLAPRAVLVHRTTEYEELVARHGTHGQAAFFLASRGRDIDEVAE
                     RHHRTRRALAEVASAVPLTWRQSRVERADLDRFLFAPEDVVVVVGQDGLVANAAKYLAG
                     QPVLGIDSDPGRNPGVLVRHRPADAAALLPAALARGTGADELTMVEAVADDTQRLVALN
                     EIYLGAAGHQTARYRLGLDGDGGVVEAQASSGVLVGTGTGATGWLRSVWQERASRLALP
                     SPGEDRLLWFVREAWPSPATGTSLVAGELAAAQALSVTVESDRLIAFGDGIESDALELT
                     WGQTIRVRVCGQRLRLVG"
     gene            complement(9539..10720)
                     /locus_tag="N8I87_RS31125"
                     /old_locus_tag="N8I87_31125"
     CDS             complement(9539..10720)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013000128.1"
                     /locus_tag="N8I87_RS31125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_31125"
                     /product="SPFH domain-containing protein"
                     /protein_id="WP_263213695.1"
                     /transl_table=11
                     /translation="MADITRRLGWRHLRGAPTAHIRHHRSGKLAHDGPGLSFWYRSLTA
                     ALSEVPVDDRELAMTFHARTSDFQDVAVQATVTYRIGDPAVAAARLDFSIDPDTGVWRG
                     APLEQLGTLLTETAQQHALAVLARTPLSSALADGVSAVRERIEEGLGNEPRLPAIGLEV
                     VAVRVMALRPEPEVERALRTPAREQIQQEADRATYERRAVAVERERTIAENELASQIEL
                     ARREEQLVEQRGTNTRREAEEHAAADAVRAQAEAARTVRLAEAEAARSVKLAEAEADRS
                     VRLADAEARRTGLLAEAEAARTARLAEAEAARSTRLARAEAEGAREVGEARAQAQAAWL
                     RVHADAGVDGVATLQALTATRLAENLPRIDSVTVSPDVLTGLLAKLGGTGTEE"
     misc_feature    10735..10755
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: medium, score: 29.81"
                     /tool="antismash"
     misc_feature    complement(10769..10789)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: medium, score: 27.43"
                     /tool="antismash"
     misc_feature    11444..11458
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 21.24"
                     /tool="antismash"
ORIGIN
        1 ccggcgtgct cgccatccag gtcgcccagg tcgaccagat gtccggcggc cgggtcgaac
       61 tcggcctggg cgcgggctgg ttcgagcagg agcacaaggc gtacggcatc cccttcccga
      121 aggagaagtt cgcccgcctg gaggagcagc tggcgatcgt caccggtctg tgggcgaccg
      181 aggccggcaa gaccttcgac ttccacggca cgcactacga cctcaccgac tcgcccgcac
      241 tgcccaagcc cgcccaggcc aaggtcccgg tcctgatcgg cggacacggc gccacccgca
      301 ctccgcgcct ggccgcgcag tacgccgacg agttcaacat gccgttcgcc tcgatcgagg
      361 acagcgagcg gcagttcggg cgggtgcgtg ccgccgcgga ggccgagggt cgcagggccg
      421 acgacctgac ctactccaac gccctcgtcg tctgcgtcgg ccgcgacgac caggaggtcg
      481 cccgccgggc cgccgcgatc ggccgcgagg tcgacgagct gaagaccaac ggtctggccg
      541 gctccccggc cgaggtggtc gacaagatcg gccggtacgc cgcgatcggc tcgcagcgca
      601 tgtacctcca ggtcctcgac ctcacggacc tggaccacct ggagctgatc tcctcccagg
      661 tccagtcgca gctgtcctag gcacaacggg cgcgggccta cgacgatcgg ccgtcgaggg
      721 catcacgacg gtcggtcgtc ggtccgctac cgtatgaaaa ccgctggtca acggcgccct
      781 ggccagggag actcagggca tgttcctgac catcggcacc accggcaccc cggagcgccc
      841 cgccaccgac ctcggctttc tgctgcacaa gcatcccgag aaggcgcagg tgttctccac
      901 ctcctacggc acggcgcacg tcctctaccc agaggcggac gcggaacgct gcacggcggc
      961 gctcctgctg gaggtggatg ccgtggcgct ggtgcggcgg ggcaagggca agggccgggg
     1021 cggagccccc gacgcggcgc tcgcgcagta tgtcaacgac cgcccgtacg ccgcctcctc
     1081 gctgctcgcc gtggcgctga gcagcgtctt ctccagcgcg atgcgcggcg tgtgcgccgc
     1141 ccgcccggag cgcgccacgc agccgctgcc gctgcgcgtg gaggtgcccg cgctgcccgc
     1201 ccgcggcggc cccgagctcg tacgacgcct cttcgagccg ctcggctgga cggtgagcgt
     1261 cgaaccggtg gccctggaca ccgcgttccc gcagtggggc gactcgcgct atgtgcggct
     1321 cgtcctggag tcggaggcgc tgaccctcgc cgaggcgctg cggcacctgt atgtgctgct
     1381 gccggtcctc gacgacgcca agcactactg ggtcgcctcc gacgaggtcg acaagctgct
     1441 gcgcgccgga gagggctggc tgcccggcca cccggagcag aagctcatca ccagccgcta
     1501 tctctcccgc cgctggtcgc tcacccggca ggcgatggag cgcctggagc tggtacgcct
     1561 cgcggaggcg gacgacagcg acgtcgagga gatcgacaac gccgtcgagg aggacagcga
     1621 ggcggaggag aagccgaccc cgctcgccgt gcagcgtcgg gacgcgatca tcgccgcgct
     1681 cggcgagtcc ggtgccgccc gggtcctcga cctcggctgc ggacagggcc agttggtgca
     1741 ggcgctgctc aaggacccgc ggttcaccga gatcgtcggc gtcgatgtgt cgatgcgggc
     1801 gctcaccatc gccggacggc ggctgaagct ggaccggatg ggagagcggc aggcctcgcg
     1861 cgtcaggctc cagcaggggt cgctcgccta cacagacagc cggctcaagg gctacgacgc
     1921 cgccgtgctc agcgaggtca tcgagcacct cgacctgccc cggctgcccg ccctggaata
     1981 cgcggtgttc ggcgccgccc ggccgcgcac ggtcctcgtg acgaccccga acgtcgagta
     2041 caacgtccgc tgggagtccc tcccggccgg gcacgtccgg cacggcgacc accgcttcga
     2101 gtggacccgc gaggagttcc gcacctgggc cggggtggtg gccgaacggc acggctacga
     2161 cgtcgagttc cggcccgtcg gacccgacga ccccgaggtg ggcccgccca cgcagatggc
     2221 cgcgttcagc atcaggacca ccaacgagaa ggaggcgaag gcggcatgac agagacacgc
     2281 aaggcacggg tgctccccgt caccgacctc tccctcgtgg tgctcgtcgg cgcctccggc
     2341 tcgggcaagt ccaccttcgc ccgccgccac ttcaagccca ccgagatcat ctcctcggac
     2401 ttctgccgcg gtctggtctc cgacgacgag aacgaccaga gcgcgacgac ggacgccttc
     2461 gacgtcctgc actacatcgc cggcaagcgg ctggccgccg gccgccgtac cgtcgtcgac
     2521 gccaccagcg tgcagcagga cagccggcgt cagctgatcg aactggcgag gaagtacgac
     2581 gtgctgccca tcgccatcgt cctcgacacc cccgaggagg tgtgcgccga gcgcaacgcg
     2641 gcgcgcaccg accgggcgga catgccgcgc cgggtgatcc agcggcacat ccgtgaactc
     2701 cgccgctcca tcagacactt ggagcgcgag ggcttccgca aggtgcacat cctgcgcggg
     2761 gtcgaggagg ccgacggcgc caccgtcgtc accgagaggc gcttcaacga cctgacccac
     2821 ctcaccggcc ccttcgacgt catcggcgac atccacggct gcgcctcgga actggagtcg
     2881 ctgctcggca agttgggcta cagcgacggc gtgcaccccg agggccgtac ggccgtcttc
     2941 gtcggcgacc tcgtcgaccg cggcccggac acccccggcg tgctgcgccg cgtgatgtcc
     3001 atggtcgcgt cgggcaacgc gctgtgcgtg cccggcaacc acgagaacaa gtacggccgt
     3061 tacctcaagg gccgcaaggt gcagcacacc cacgggctcg ccgagaccat cgagcagatg
     3121 gagggtgaga gcgaggagtt cacggcacag gtcaaggagt tcctcgacgg cctcgtcagc
     3181 cactacgtcc tcgacggcgg ccggctcgtt gtctgccacg cgggtctgcc ggagcggtac
     3241 cacgggcgga cgtccggccg ggtgcgctcg cacgccctgt acggcgacac gacgggggag
     3301 acggacgagt tcgggctgcc ggtgcgctac ccgtgggccg aggactaccg gggccgggcc
     3361 gccgtggtct acggccacac accggtgccg gaggccacgt ggctcaacaa caccatctgc
     3421 ctggacaccg gtgccgtctt cggcggcaag ctcaccgcgc tgcgctggcc ggagcggcag
     3481 ctggtcgacg taccggccga gcgggtctgg tacgagccgg ccaagccgct gcgcagcgag
     3541 gcgcccggcg ggcacgacgg gcggccgctg gacctggcgg acgtgcacgg ccgaagggtc
     3601 gtggagacac ggcacgcggg ccgggtcgcg gtccgcgagg agaacgcggc ggcggccctg
     3661 gaggtcatga gccgcttcgc gatcgacccg cgcctgctgc cgtacctccc gccgaccatg
     3721 gcgccgacgg ccacgagtca catggagggc tacctggagc acccggcgga ggccttcgcg
     3781 cagtacgcgc aggacggcgt cgcccgggtc gtgtgcgagg agaagcacat gggctcacgg
     3841 gccgtggccc tggtgtgccg ggacgccgag gcggcacgca agaagttcgg cgtggacggt
     3901 cccaccgggt cgctctacac ccgtaccggc cgtcccttct tcgacgacga ggccgtgacc
     3961 gaggagatcc tcggacgcgt ccgctcggcg atcggcgagg cgggcctctg ggacgaactc
     4021 gacacggact ggctgctgct cgacgccgag ctgatgccgt ggtcgctgaa ggcgtccgga
     4081 ctgctgcgtt cgcagtacgc ggccgtgggt gccgcctccg gcgcggtgtt ccctggcgcg
     4141 ctggccgctc tggagggcgc ggcggcacgc ggtgtggacg tgaccgggct gctctcccgg
     4201 cagcgcgagc gggccgccga cgcggccgcg ttcacggacg cgtaccgccg ctactgctgg
     4261 accacggacg gcctggacgg ggtgcgtctc gccccgttcc agatcctggc cgtacaggac
     4321 cgcagcctgg ccgcgctgcc gcacgacgag cagctcgcgc tgatcgaccg gctcgtcgag
     4381 cacgacggca ccgggctgct gcagaacacc cggcggctgt acgtcgacac cggcgacccc
     4441 gagtcggtgc gggcgggtgt cgactggtgg ctggagatga ccggccgggg cggcgagggc
     4501 atggtcgtca agccggtcgg cgcggtggtg cgcagtgacg agggccgtct ggtccagccg
     4561 ggcatcaagt gccggggacg tgagtacctg cggatcatct acggacccga gtacacccgt
     4621 ccggacaacc tggaccggct gcgcaagcgg ttcctcaacc acaagcggtc actcgcgatc
     4681 cgcgagtacg cgctggggct ggaggccctg gaccggctgg cggagggcga gccgctgtgg
     4741 cgggtgcacg aggccgtgtt cggggtgctg gccctggagt cggagccggt ggacccgcgc
     4801 ttgtgaaccg tcccacccgc ccttgtgcct cacacgtatg tgtccatatg gtgaaacgcc
     4861 ttgcgacgta cgtgtgttcg gaacaccgga gggagtgagg gacatggaga tcaagaaggt
     4921 ggtcgtccgc gaggaccgga gcaccagccg ccggccggcg atcctggaga agcggccgac
     4981 ccgctgaccg aaggggcggt atgcgcgttc acctggtgac gatgccctgg catccgatcg
     5041 acctcccctc gctgcaagtg ggcctgctgc accgactggt gcggcgggcc cgcccggccg
     5101 acgaggtgac cgagttccac ggctccctgc gctgggccga gttcctcctg gagcactccg
     5161 gaggacggct gcgtccgggc gactatgtgg cggtcggcag cgactcgatc ttccacggcc
     5221 tcggcgactg ggtgttctcg ggcgtcctgt acgacgaccc cgactggggc tcggcccggc
     5281 tgcgcgccta cgccgcggac cgggacgtct tcatcgacac cgccctcgag atgcggccgt
     5341 acgcggccgg attcatcgac gagtgcgccg ccgaggtgct cgcggccgag cccgacgtgg
     5401 tcgggttcac caccacgttc atgcagaacg tgtcctccct ggcgctggcc cgtgagctca
     5461 agcgccgccg cccggagctg accgtcgtct tcggcggcag caactgcgac ggccccatgg
     5521 gccacgccct gcaccgcaac caccggttcg tggaccatgt ggtgcgcggc gagggggagt
     5581 acgccttacc ggcgctcctg cggcatgtgg acgcgggcac cccgcccgtg gacgtccccg
     5641 gcctgtgctg gtgggacggg gacatgtccc gggccaacac cgagacccgg cgcacggtgg
     5701 cgcccgccga cataccctca cccgactacg accagtggca ggccgccctg gagccctcgc
     5761 cggtgctgga gtacgtccac cccaaactgg tggtcgaggg ggcccggggc tgctggtggg
     5821 gcgagaagca ccactgcacc ttctgcgggc tcaacggctc gtccatggcc tttcgcgcca
     5881 agccgggcga gcggctgtgg gaggagatcg accgcctcgt acgacggcac cgcatcctcg
     5941 acatcgtcac cgtcgacaac atcatcgaca tggcctactt ccgggacttc ctcccgcgtg
     6001 tcgtcgacag cggctgggac ctgcggctgc actacgaggt caagtccaac ctcacccccg
     6061 accagctccg cctcctcggc cgctcgggca ccgtgcacat ccagcccggc atcgagagcc
     6121 tgggcagccg cgtgctcgac ctcatggaca agggcgtgag cggcgcccgc aacatccgca
     6181 ccctccggga gtgcgagaac cactcgctca cctgctcctg gaacctcctg tacggcttcc
     6241 ccggcgagag tgccgaggac tacgcccatg tcgtcgacca gctcccggcg ctcgtccatc
     6301 tgcagccgcc cagcggcgcc caccgcatcc agctggagcg gttcagcccg cacttcaccg
     6361 accgggccct gggcttcggc aagcggcgcc cggccgcgat gtaccagcac gtctacgacc
     6421 tgcccgagga cgaactggcc gacctcgtct acctgttcga cacggaggac gcgggcatcg
     6481 ggggggagac ggagcaccgg ctcaaggcgg ccgtggcgca gtggcgcgcc ggacatcaca
     6541 cctcacggct gctgttcgag gaaaccgagg gagacgaagg agggggagcg gacgccctcc
     6601 tggtgcacga ccggcgccac ggccggccgt cggccaccca ccgcttcacc ggctgggcgg
     6661 cggccgcgct gcgccgcctg gaggacgggc gcaccgagtc cgcgctgcac cggctgctga
     6721 cggcggacgg ccactccgtc agccccgaag ccctgcgtga atggctccaa cacaccttcg
     6781 ccctaggcct gttgttccgt gacggccgga cctatgtctc cctgcccacc tgggacgaga
     6841 cggtccgtgt gcccgacggg gcaggggtcg ccggatgacc gggctgccca ccgccgtccc
     6901 gcaccgggtc accggccgcc gggtcgaggt gaccgagccg cttcgcctgg gcgcgggcgg
     6961 gcaacgcact gcggtggcag tgcagttcct gcgcgagtgc cagagcctcg ggctccgggt
     7021 gggctggacg gcggccgaca gggagacgcc gtacgacatc gggctgctgc gccatctgtc
     7081 cccgcccgct caagtgtccg atgagccagg tgaattgaga gagtggcggg cggggtatgc
     7141 gtacgggatg ctctaccacc gccgtggccc ggacttcgtc gccgtcatgg accgcaggga
     7201 gcccggcacc gccgtacggc tcaccctcga ccaccccgat ctgctctccg ccttccacac
     7261 cctgctgaac cccacgccgc tcgacgaact ggaccccggc ccgcgcgagg ccgcgggact
     7321 gctggccgca gagcggctgg ccctggtcac cgacggatgg gcggtggccc tgccgccacg
     7381 tatcggccgc tggcccgtgc cctgcacggg gatctgaggg gtggccgcgg caaccgccga
     7441 ggcccggccg ctcccgctgc ggcggcagcg ggccttcgtg ctgctgtgcg gcgagcagat
     7501 cgccggctcc gtcggacgcc aggtcaccgc cctcgcggtg gcgctgctcg cggtgaccga
     7561 tctgggcacg gggccgttcg gggcggccgc ggtgctcgcg ctgacctatc tgccgggggc
     7621 cctgctcagt ccgttcgccg gagtgctcgt cgaccgggtg cggctgcgcc ggctgctcgt
     7681 cgtggtcacc gccgcacagg cactggtcgt gggctcggtc ccgctcgtga acaccctggg
     7741 cggagtcggc cgtccacagc tgtacggcgt cgccgtcgtc tccggcgcgc tgaccttcac
     7801 cctctcggtc gccctgcaga cggctctgcc ccgggtcgtc gcgccggacc ggctgctgtc
     7861 cgccaactcc gcgctcaccg gggcccgtac ggccggactg atcggcggcc cggcactcgg
     7921 cggtgtgctg gtcggaatcc tcggccccgc cccggccctg ctggccggca gtgcggccta
     7981 cgccgtcgag gcgttgctcc tgctggccct gcccgcggca ctgaacgacc cggccgaacc
     8041 gaggggcgcg cagtcgccgg cctccctgct ccgcacggga cttgaggtgc tgcgcggtga
     8101 accgaggctg cgcctgatgg tcctcgcggg cgccgggctc aacctcggca gcggtgcgtc
     8161 gggcgcgctc ctcgtgttct acgcgacccg tgatctcgcc ctttccgcct ggcagttggg
     8221 catttcgtac gccgtgcaca gcgcggccac gctggcgggc gtgggtgtcg ccggccgctt
     8281 cgcccggacg gcggggctgt ggtcggccac caggatctgc gccctgctgg cgggcggcgc
     8341 ggtctttctg atcccggcgg cctcgctggg ccccgcgttt gagctgctgc tcggctacca
     8401 ggcctgttac gggctcgccg tcaccgtctg ggcgatctcc atgaccacgc tgcagcagct
     8461 cgtcacaccg gacggcatgc gcggccgtgt cgccgggttc gtacaggctg ccctcctcgg
     8521 gacggtgccg gtcggagcgc tggcgggcgg cgcgctggcc gccgggatcg gcaacgtgcc
     8581 cgtgctcctc gggtcggcgg tggtggcgct gctggcggcg gcgagcctgt ggacgccggg
     8641 cgtcagccga ccagccgcag tcgctgcccg cacacccgaa cccggatcgt ctgcccccag
     8701 gtgagctcca gcgcgtcgct ctcgatcccg tcgccgaagg cgatcagccg atcggactcg
     8761 acggtgaccg acagggcctg ggcggccgcg agttcgcccg ccaccaggga cgttccggtg
     8821 gccggtgacg gccaggcctc ccgtacgaac cacagcagcc ggtcctcgcc gggggagggg
     8881 agcgcgagcc ggctggcccg ctcctgccac accgaccgga gccacccggt ggccccggtc
     8941 ccggttccca ccagcacccc ggacgaggcc tgggcctcga cgacaccccc gtcgccgtcg
     9001 aggcccaggc ggtaccgggc ggtctgatgt ccggccgccc ccagatagat ctcgttgagc
     9061 gcgaccaggc gctgtgtgtc gtccgcgacg gcctcgacca tggtgagctc gtcggcgccg
     9121 gtgccccggg ccaaagcggc cggcagcagc gccgccgcgt ccgcgggccg gtgccgtacg
     9181 agcacaccgg ggttgcggcc cggatcggag tcgatgccca gcaccggctg gcccgcgagg
     9241 tacttggcgg cgttcgccac cagcccgtcc tggcccacca ccacgaccac gtcctcgggc
     9301 gcgaacagga aacggtccag gtcggcccgc tccacccggc tctgccgcca ggtcagcggg
     9361 accgccgagg cgacctcggc cagcgcgcgg cgcgtgcggt ggtggcgttc ggcgacctcg
     9421 tcgatgtcac ggccgcggga ggccaggaag aacgcggcct ggccgtgcgt gccgtgccgg
     9481 gccaccaact cctcgtactc cgtggtgcga tggaccagca cggcacgcgg ggcgagactc
     9541 attcctccgt cccggtaccg ccgagcttcg ccagcagccc ggtgagcacg tccggcgaca
     9601 cggtcacgct gtcgatccga ggcaggttct ccgccagccg ggtggcggtc agggcctgca
     9661 gcgtcgcgac accgtcgact ccggcgtccg cgtgcacccg cagccacgcg gcctgcgcct
     9721 gtgcccgcgc ctcacccacc tcgcgcgccc cctcggcctc ggcgcgggcg agtcgtgtgg
     9781 atcgggccgc ctccgcctcc gccagccggg ccgtccgcgc ggcctccgcc tcggcgagca
     9841 gcccggttcg ccgcgcctcg gcgtcggcca gtcgcaccga ccggtccgcc tccgcctccg
     9901 cgagcttcac cgaccgggcc gcctcggcct cggcgagccg gaccgtccgg gccgcctccg
     9961 cctgggcgcg caccgcgtcg gccgccgcgt gctcctcggc ctcgcggcgc gtgttcgtgc
    10021 cgcgctgctc gaccaactgc tcctcgcggc gggcgagttc gatctggctg gccagctcgt
    10081 tctcggcgat cgtccgctcc cgctcgacgg ccaccgcccg ccgctcgtag gtggcccggt
    10141 cggcctcctg ctggatctgc tcacgagccg gggtgcgcag cgcccgctcc acctcgggct
    10201 cgggccgcag cgccatcacc cggacggcga cgacctcgag gccgatggcc ggcagccggg
    10261 gctcattgcc cagcccctcc tcgatccgct cgcgcaccgc gctcaccccg tcggccagcg
    10321 ccgacgacag cggcgtacgg gcgagcacgg ccagcgcgtg ctgctgcgcc gtctcggtga
    10381 gcagcgtgcc cagctgctcc aggggcgcgc cccgccacac gcccgtgtcg gggtcgatcg
    10441 agaagtccag acgggcggcc gcgacggccg gatcaccgat ccggtaggtg accgtcgcct
    10501 gcacggccac gtcctggaag tcggacgtgc gggcgtggaa ggtcatcgcc agttcacggt
    10561 cgtcgaccgg cacctccgag agcgcggcgg tcagcgagcg gtaccagaag ctgagccccg
    10621 ggccgtcgtg cgcgagcttc cccgaccggt ggtgtctgat gtgcgccgtc ggggcgccgc
    10681 gcagatggcg ccagccgagg cgcctggtga tgtcggccat gggaccccct cctattttcg
    10741 tcatatagac gataaacgga ggaggtgctt gtcgtcaacg ggacgaaaaa caagaagatt
    10801 ccgatacggt cgagcagagg gtgaatgcgg tcctcaatgg tcaggatgga gacatgggat
    10861 tccacgtcga ctccgaggcc gggcggctgc gccgcgtcat cctgcaccgg ccggacctcg
    10921 agctcaaaag gctcaccccc agcaacaagg acgcacttct cttcgacgac gtgctgtggg
    10981 tgcgccgggc gcgcgccgag catgacgggt tcgccgacgt gctgcgcgac cgcggcgtca
    11041 ccgtccatct cttcggtgac ctgctcgccg agaccctgga gatccccgag gccaggacgc
    11101 tcgtcctgga ccgggtcttc gacgagaagg agtacggccc cctcgccacc gaccacctcc
    11161 gggcggcctt cgagaccctg cccgcgcgcg acctggccgc ggccctggtc ggcggcatga
    11221 ccaagcggga gttcctggac gcgcacgagg agccgacctc cgtccgcttc catgtgatgg
    11281 acctggacga cttcctcctc ggcccgctcc cgaaccacct cttcacccgg gacacctctg
    11341 cctggatcta cgacggggtc gccatcaacg ccatgcgcct gcccgcccgg cagcgcgaga
    11401 ccgtgcactt cgaggcgatc taccggcacc atccgcagtt ccgggacgaa gcgttccgtg
    11461 tctggtcggc gggccaggcc gactacccgt ccaccatcga gggcggcgac gtcctggtga
    11521 tcggcaacgg cgccgtcctg atcggcatga gcgagcgcac caccccgcag gccgtggaga
    11581 tgctcgcgca caagctgttc gcggcgggct cggcgcgcac gatcgtcgcg ctcgacatgc
    11641 ccaagcggcg cgccttcatg cacctcgaca ccgtgatgac gatggtcgac ggcgacacct
    11701 tcacccagta cgcggggctc ggcatgctcc gctcgtacac catcgaaccc ggcgccgtgg
    11761 acaaggagct caaggtcacc gaccacccgc cggagcacat gcaccgggcg atcgccgcct
    11821 ccctcgggct gagcgagatc cgcgtcctga ccgccaccca ggacgtgcac gcggccga
//
