LOCUS       NZ_CP106798            22680 bp    DNA     linear   CON 01-NOV-2023
DEFINITION  Streptomyces sp. HUAS 15-9 chromosome, complete genome.
ACCESSION   NZ_CP106798
VERSION     NZ_CP106798.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. HUAS 15-9
  ORGANISM  Streptomyces sp. HUAS 15-9
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9258818)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 169.8x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 03:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,774
            CDSs (total)                      :: 8,679
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 504
            CDSs (without protein)            :: 504
            Pseudo Genes (ambiguous residues) :: 0 of 504
            Pseudo Genes (frameshifted)       :: 260 of 504
            Pseudo Genes (incomplete)         :: 328 of 504
            Pseudo Genes (internal stop)      :: 29 of 504
            Pseudo Genes (multiple problems)  :: 107 of 504
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:51:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4438395
            Orig. end                         :: 4461075
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106798.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22680
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /product="darobactin"
                     /region_number="13"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /rules="((cds(PF04055 and (TIGR04085 or SPASM)) and
                     darobactin_precursor) or darobactin_rSAM)"
                     /tool="antismash"
     cand_cluster    1..22680
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /detection_rules="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /kind="chemical_hybrid"
                     /product="redox-cofactor"
                     /product="darobactin"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..22680
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12680](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12680
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     protocluster    282..21511
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10281:11511](+)"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /neighbourhood="10000"
                     /product="darobactin"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      10282..11511
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /neighbourhood="10000"
                     /product="darobactin"
                     /protocluster_number="2"
     misc_feature    963..977
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.89"
                     /tool="antismash"
     gene            1033..2307
                     /locus_tag="N8I87_RS20540"
                     /old_locus_tag="N8I87_20540"
     CDS             1033..2307
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671067.1"
                     /locus_tag="N8I87_RS20540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20540"
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="WP_263210637.1"
                     /sec_met_domain="Fe-ADH (E-value: 1e-116, bitscore: 388.7,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLMTGAVTTGGTDPSADWSRDERQGELLGPQETSSDAVRRSARL
                     AKFHVPEVVFGPGSLTELGHCALRLGGRRPFLVTDPGVLEAGWTDEAVGHLRRVGLRPV
                     VWHDVTPNPKDHEVQAAFERYAQSGCDVIVGVGGGSVIDAAKGVAILSGNGGHILDYEG
                     VDQVVQPIPPTVMVPSTSGTGADVSQFVIITDTTQQIKISIVSRTLVPDISVIDPRLLT
                     TMPDWLNAATGLDALTHAVEAFVSRAHNPLTDIHALHAVELITGNLVRTQVQPKNFGAR
                     LAMAQASLEAGMAFTNAILGATHAMSHQVGGLLDAPHGVVNGVLLPHVIRFNAAAWPER
                     FVRLGAAAGLPTDGVPPYEVAERLADLVRALADDLGVPKSLASLGVAEHDVPVLARTTL
                     RDACMATNPRDVDVRDVERLFREAL"
     gene            2318..3688
                     /locus_tag="N8I87_RS20545"
                     /old_locus_tag="N8I87_20545"
     CDS             2318..3688
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019350.3"
                     /locus_tag="N8I87_RS20545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20545"
                     /product="histidine kinase"
                     /protein_id="WP_263210638.1"
                     /transl_table=11
                     /translation="MSVPDRYPQTADLGVLTGLRSGKRSYYPAYVRSAERLERTVEALD
                     GISRALVRTAEGPRALVEAVVRTAAEHLQARWLLLAVADGALRAARPRFLLYADDLLID
                     NESRVPAEVREHLALLRSHPWEAGTPDRGRDWVRAPMTLDDEPVGGIAAAPGPGVEVAD
                     TDLAILRVLANQAAVALHHTFLFHAATTLRGRAEELSEAAERQARDLAARSAELAETQA
                     RLLEALQRQALDDERRRIARELHDSVSQYVLSAGMTVEVCRAELAALGGPAADIAERLA
                     GAKDLNRQAVERLRAAIYALQHTSQEPTGPLPVMLERLSTVHLTTDLDVRVQVTGSPVP
                     LPPEADQSILRLTGEALFNSATHGKAGRALVRLRYLPEAVVLTVSDDGCGDPAQLRRAL
                     RLSRAADLGGRHRGLANMVTRAEEFGGRLSIRRSGMGGVLLRVGIPLPVKHCPGGSR"
     gene            3685..4350
                     /locus_tag="N8I87_RS20550"
                     /old_locus_tag="N8I87_20550"
     CDS             3685..4350
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493196.1"
                     /locus_tag="N8I87_RS20550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20550"
                     /product="response regulator transcription factor"
                     /protein_id="WP_263210639.1"
                     /transl_table=11
                     /translation="MTTQQSAAAPTQAQRIVLVDDHAIVRHGLRSILERESDLEVVGEA
                     STAAEAAAVVERTDPSIVLLDLKLSTAADTEGLDLCARLTQRYPDLAVLVLTTFLDDSL
                     VVQAIQHGARGYVVKDVDTTELLRSIRAVARNESAFDSHAAAAMVRSMRVQPDRPKFTD
                     RELKVLRLLAHGLSNREIGAELYISETTVKFHVRNIMRKTEAGSRAEVVYEATKMGVI"
     gene            complement(4370..5296)
                     /gene="mftM"
                     /locus_tag="N8I87_RS20555"
                     /old_locus_tag="N8I87_20555"
     CDS             complement(4370..5296)
                     /codon_start=1
                     /gene="mftM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671064.1"
                     /locus_tag="N8I87_RS20555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20555"
                     /product="mycofactocin oligosaccharide methyltransferase
                     MftM"
                     /protein_id="WP_263210641.1"
                     /transl_table=11
                     /translation="MSLPSLRSAIAARRIDPLAPCPSGAYADHLVRVIRDSPRPDDATP
                     RPIVRTEHFTLRRRGRRVELGHSLRPEHLDNDLAGLLAEELFAPGWLSGSDIFERVFTG
                     VVRSCVDGALPAWTTFYDNTLARIRESWRAPKPADHSSIAGFAPVYRRTLDLVAPGTVL
                     DMGSCFGFLPLLMAEQQGHTVIASDLSRGTMRLLAAVAGARGLALDTLACDAAQVPLPD
                     RSVDTVTVIHVLEHLDPGHGDTVVREALRLAGQRVVVAVPYEDEPTAAYGHVRCFDPAG
                     LTALGRRTGHRFSVTSHHGGWLTLETS"
     gene            complement(5293..6816)
                     /locus_tag="N8I87_RS20560"
                     /old_locus_tag="N8I87_20560"
     CDS             complement(5293..6816)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671063.1"
                     /locus_tag="N8I87_RS20560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20560"
                     /product="VWA domain-containing protein"
                     /protein_id="WP_263210642.1"
                     /transl_table=11
                     /translation="MHRFVRVLRLFGLRVSVSEAMDAMRAAAQPGVLGRRETLREALRL
                     TLVKDRRDDELFDELFAAFFSLRPVHQDSDGHGHSHEHDDLTDTGELESYTVSDEPSET
                     PQQGHSHGKPADIRDYFDQQDLAQRYNLHQEANKLDLAAMTEEIVLSKDGAADATRGPS
                     VQLETERLHGAGIPGQLAAAAGHPIDTTLTVAQQDALLGWLADDTSDDPDDPGPGALRR
                     GPAGVIDNLPELLRRHLDRLAELQSVAVESAQLAERARAEAVDEHERTQLEEALRRVGR
                     SLHGALTSRKRATPRGRVHPARTMRRNMRYDGVPFRPVTVTRAEDRPRLVILTDVSLSV
                     RATARFTLHLVHGLQSLFGQVRSFAFVDEPTEITELFTEHPLERALGLVFDGLPAGGLL
                     DVDANSDYGRTFELLLAEHAPALNRRTTLLVLGDGRGNGNPSGLAAFEEITRRVRETIW
                     LTPEPRFSWGLGSCDLPLYAEYCARVQVVADLAGLERTARRMATEVTGR"
     gene            complement(6885..8069)
                     /locus_tag="N8I87_RS20565"
                     /old_locus_tag="N8I87_20565"
     CDS             complement(6885..8069)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671062.1"
                     /locus_tag="N8I87_RS20565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20565"
                     /product="MoxR family ATPase"
                     /protein_id="WP_263210643.1"
                     /transl_table=11
                     /translation="MPATPARPAEFASVEDITERFAAHGYIADRRLATTVFLLTRLDKP
                     VLLEGPAGVGKTELAKALAAATGRRLLRLQCYEGQDESRALYEWDYGKQLLYTQMLRDK
                     IGDLTADAPDLASAVQRIEEQDSVFFSDRFLAARPLLEAVRGEEPAVLLIDEVDRADEA
                     LEAVLLECLAEYQVSVPEIGTYTAKVPPYVVLTSNNTRDLSAALKRRCLHLFLDYPDAE
                     RELEILRAKDTGLSEATARHLVEVVRGLRELQLRKNPSISETVDWARTLAVLGVEELDP
                     EVLADTANVVLKYERDLGRALDVLPRLVDPNRTLPEPGHRHDGHGHHHHNGHHHDHDHE
                     HEVDGRAVRAAMDQQGRHDDGYYGAPGPGPTRRTTPGAGQGARAFAAIARRRAL"
     gene            complement(8177..9469)
                     /gene="mdo"
                     /locus_tag="N8I87_RS20570"
                     /old_locus_tag="N8I87_20570"
     CDS             complement(8177..9469)
                     /EC_number="1.1.99.37"
                     /EC_number="1.2.98.1"
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0046170 - methanol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mdo"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662116.1"
                     /locus_tag="N8I87_RS20570"
                     /note="This methanol dehydrogenase is considered a
                     nicotinoprotein, since its NADP cofactor remains is not
                     dissociable, but instead remains permanently bound. A
                     member of this family has been shown to act as a
                     formaldehyde dismutase, able to convert two molecules of
                     formaldehyde (plus one water molecule) into one of methanol
                     and one of formate, with no net change in its redox state.
                     More recently, it was shown in Mycobacterium smegmatis that
                     this enzyme is critical to ethanol utilization, for which
                     the biosynthesis of the cofactor-like electron carrier
                     mycofactocin is also required.; This methanol dehydrogenase
                     is considered a nicotinoprotein, since its NADP cofactor
                     remains is not dissociable, but instead remains permanently
                     bound. A member of this family has been shown to act as a
                     formaldehyde dismutase, able to convert two molecules of
                     formaldehyde (plus one water molecule) into one of methanol
                     and one of formate, with no net change in its redox state.
                     More recently, it was shown in Mycobacterium smegmatis that
                     this enzyme is critical to ethanol utilization, for which
                     the biosynthesis of the cofactor-like electron carrier
                     mycofactocin is also required; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="N8I87_20570"
                     /product="NDMA-dependent methanol dehydrogenase"
                     /protein_id="WP_263210645.1"
                     /sec_met_domain="Fe-ADH (E-value: 3.3e-107, bitscore:
                     357.4, seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQVEELLKPFPIKEFHPFPRALMGPGAHELIGPEAKKLGFRRTLV
                     MTTGLRGSDTVHKIVESLKYHGLEVVVYDKVESNPKDYNVMDAVALYQENKCDSFVSIG
                     GGSSHDACKGARISVAHDGRNVNDFEGFNKSENPKNPPHIAVSTTAGTGSETSWAYVIT
                     DTTTDPDNPHKYVAFDDASVASLAIDDPTLYYDCPLDYTAQCGFDVLAHASEPYVSRLN
                     FQPSLGNALHAIRLTARHLRQAVWNGTDLAGREGMMYAQYIAAQAFNSGGLGIIHSISH
                     AVSACYDTHHGLNNAIALPRVWAFNMPAQYERFADIAEAMGVDTHGMTKVQAADAALAA
                     GIRLLRDVGIPEKFTDVQQDTYSKSRLGQGPTKYYEQAKVIKGDAADVDRITNHVLGDA
                     CTPGNAKECTFDTVRPVVDHCMNGDLDELLG"
     misc_feature    complement(9432..9445)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            9864..9986
                     /gene="mftA"
                     /locus_tag="N8I87_RS20575"
                     /old_locus_tag="N8I87_20575"
     CDS             9864..9986
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671060.1"
                     /locus_tag="N8I87_RS20575"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis.;
                     Mycofactocin is a small molecule electron carrier derived
                     from the final two amino acids, Val-Tyr, of MftA, the
                     mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20575"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_263210646.1"
                     /transl_table=11
                     /translation="MSAVQDDTLVAEQDDTVNADDVIEEILVEEVSIDGMCGVY"
     gene            10001..10285
                     /gene="mftB"
                     /locus_tag="N8I87_RS20580"
                     /old_locus_tag="N8I87_20580"
     CDS             10001..10285
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068047.1"
                     /locus_tag="N8I87_RS20580"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA.; MftB, a small
                     protein, is a peptide chaperone that assists the radical
                     SAM enzyme MftC in performing two modifications to the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     peptide, MftA. MftB's role is analogous to the role of PqqD
                     in the biosynthesis of PQQ, a cofactor that derives
                     entirely from a Tyr and a Glu in the precursor PqqA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20580"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_263210647.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 9e-27,
                     bitscore: 92.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSPFDADRPYRLNPCVALRPEPFGALAYHFGNRRLSFLKAPELV
                     ELVRELGRHASAREALAGVPEARRDTFRRALASLAAADMIIPTGAEPQP"
     aSDomain        10022..10255
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_N8I87_RS20580_Mycofactocin_RRE.1"
                     /evalue="3.00E-29"
                     /identifier="RREFam016.1"
                     /label="N8I87_RS20580"
                     /locus_tag="N8I87_RS20580"
                     /protein_end="85"
                     /protein_start="7"
                     /score="92.1"
                     /tool="antismash"
                     /translation="DRPYRLNPCVALRPEPFGALAYHFGNRRLSFLKAPELVELVRELG
                     RHASAREALAGVPEARRDTFRRALASLAAADMI"
     gene            10282..11511
                     /gene="mftC"
                     /locus_tag="N8I87_RS20585"
                     /old_locus_tag="N8I87_20585"
     CDS             10282..11511
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: darobactin_rSAM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671058.1"
                     /locus_tag="N8I87_RS20585"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="N8I87_20585"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_263210648.1"
                     /sec_met_domain="PTHR43273 (E-value: 1e-37, bitscore:
                     128.9, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 5.9e-26, bitscore: 90.7,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1.9e-07, bitscore: 29.7,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.1e-13, bitscore:
                     50.2, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 7.2e-05, bitscore: 22.1,
                     seeds: 166, tool: rule-based-clusters)"
                     /sec_met_domain="darobactin_rSAM (E-value: 1.0, bitscore:
                     1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVTQEPSAPVPALVEQFKGGLHAPICMTWEWTYACNLSCVHCLS
                     SSGRRDPAELSTDEIKSVLDELRRMQVFYVNVGGGEPTVRPDFWELLDYAIAHQVGVKF
                     STNGLRLTPDRARRLAATDYVDVQISLDGATREINDAVRGRGSYDMAVRAMENLAAAGF
                     RDFKISVVMTRQNIPQLDDFASLADRYGAQLRITRLRPSGRGADVWDELHPTAQQQREL
                     YTWLVGHGEKVLTGDSFFHLNALGSQPLPGLNLCGAGRVVCLIDPVGDVYACPFAIHDA
                     FKAGNVRAPGGFERVWRDSALFTDLRRPASGGACASCPAYDACQGGCMAAKFFTGLPLD
                     GPDPECVMGHGATALTATERSALPPQGNDHSHRTVPLGMPAVRTRRPDRFCDESPLAGA
                     DAVHHTKGTS"
     gene            11508..12680
                     /gene="mftD"
                     /locus_tag="N8I87_RS20590"
                     /old_locus_tag="N8I87_20590"
     CDS             11508..12680
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658591.1"
                     /locus_tag="N8I87_RS20590"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="N8I87_20590"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_263210650.1"
                     /sec_met_domain="TIGR03966 (E-value: 2.1e-212, bitscore:
                     703.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKWIETVAEAQRLAKKRLPKSVYSALLAGSEKGVSYDDNTTAFG
                     QLGFAPHVAGLSAKRDLATTVMGQQIALPVIISPTGVQAVHPDGEVAVARAAASRGTAI
                     GLSSFASKPLPDVVAANPQTFFQMYWMGSKDEMQRRIEYARQAGAKGLVVTLDWSFSYG
                     RDWGSPKIPERLNLKAMAQFAPETLARPRWLWDFVKSRSLPDLSTPNLAEPGKDAPTFF
                     GAYGAWLQTPPPSWDDVRWLREQWDGPFLLKGVCRVDDALRAVDAGVTAISVSNHGGNN
                     LDATPAPIRALPAVADAVGDQVELLLDGGIRRGSDVVKALALGARAVMIGRAYLWGLAA
                     GGQTGVENVLDILSRGIDSALLGLGRSSVHELTPDDLVIPPGFTRTLGAV"
     gene            12682..13455
                     /gene="mftE"
                     /locus_tag="N8I87_RS20595"
                     /old_locus_tag="N8I87_20595"
     CDS             12682..13455
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671056.1"
                     /locus_tag="N8I87_RS20595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20595"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_263210652.1"
                     /transl_table=11
                     /translation="MAAGPARLTDLAWPDLMARRPLVLLPLGSCEQHGPHLPLDTDTAV
                     AAAVAERAAARLGKELDVLVAPAQPYGASGEHEGFPGTVSIGHDALRLLLTETGRSLLR
                     WAGRLLVVNGHGGNMVSLADAVTGLRGESRDAAWWPCVPSGPAADTDAHAGRTETSLML
                     RLRPAAVRGERAVAGPTDPVHLLMDRLVAESVRGVSPSGVLGDPAGARASEGERLLDRM
                     ADRLAADIRAWRVTGQGRLGLPDRQPVTARTGDGR"
     gene            13452..14237
                     /locus_tag="N8I87_RS20600"
                     /old_locus_tag="N8I87_20600"
     CDS             13452..14237
                     /NRPS_PKS="Domain: PKS_KR (5-176). E-value: 2.4e-14. Score:
                     45.6. Matches aSDomain:
                     nrpspksdomains_N8I87_RS20600_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671055.1"
                     /locus_tag="N8I87_RS20600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20600"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_263210653.1"
                     /transl_table=11
                     /translation="MTGRRVAVVTGAARGIGAATVRRLAREGWAVVAVDRCEDDPEVPY
                     PLADPEQLDRLTHEFPLVRTARADVRDAAALRAAVEQAEREFGGVDAAVAAAAVMLGGG
                     PVWEHTDAQWRTLLDVDVMGVANLARAAVPALLRGPQPRTGRFVALASAAAHRGLWGLG
                     AYCAAKHAVVGLVRGLACDLRDTGVNAVAVSPGSTRTDMLRATADLYELPEVDKLAEQQ
                     LSGRPLEPEEVAAVVAWACSADSFAVTGSVLHADGGFTT"
     aSDomain        13467..13979
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N8I87_RS20600_PKS_KR.1"
                     /evalue="2.40E-14"
                     /label="N8I87_RS20600_PKS_KR.1"
                     /locus_tag="N8I87_RS20600"
                     /protein_end="176"
                     /protein_start="5"
                     /score="45.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAVVTGAARGIGAATVRRLAREGWAVVAVDRCEDDPEVPYPLADP
                     EQLDRLTHEFPLVRTARADVRDAAALRAAVEQAEREFGGVDAAVAAAAVMLGGGPVWEH
                     TDAQWRTLLDVDVMGVANLARAAVPALLRGPQPRTGRFVALASAAAHRGLWGLGAYCAA
                     KHAVVGLV"
     CDS_motif       13473..13535
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N8I87_RS20600_0001"
                     /evalue="3.60E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="N8I87_RS20600"
                     /protein_end="28"
                     /protein_start="7"
                     /score="13.2"
                     /tool="antismash"
                     /translation="VVTGAARGIGAATVRRLAREG"
     CDS_motif       13890..13955
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N8I87_RS20600_0002"
                     /evalue="1.10E-03"
                     /label="PKSI-KR_m4"
                     /locus_tag="N8I87_RS20600"
                     /protein_end="168"
                     /protein_start="146"
                     /score="11.5"
                     /tool="antismash"
                     /translation="FVALASAAAHRGLWGLGAYCAA"
     gene            14560..16164
                     /gene="mftF"
                     /locus_tag="N8I87_RS20605"
                     /old_locus_tag="N8I87_20605"
     CDS             14560..16164
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671054.1"
                     /locus_tag="N8I87_RS20605"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20605"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_263210655.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.5e-22,
                     bitscore: 78.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRFLRDADPSRSVGRAGALLFAGHVGSPPRSEPLPSPPKDRLPD
                     GFAVELAADTHRSRDGHLMLGGSPPRLLRLGAGAVRLLEDGGFGVADAASAALARRLLD
                     AGVAHPRPPLPPVRDTTLAIPIRDRADQLDALLAALRADPPTSTLPVLVVDDGSRDPDA
                     LARVARRHGARLLVHPRNLGPAAARNTALRHAETAYVAFCDSDVVPEPGWLAPLLAHFG
                     DPAVALVAPRIVALPVPAPRRLDRYEQVRSPLDMGSREGPVVPVSMLGYVPSATIVVRR
                     TAVGAGFAPGMRVGEDVDLCMRLHEAGWRLRYVPDVRVGHRHRTDLRRWLAQRAGYGTG
                     AADLALRHPGRVPPLYAAPWSLAACALLLRGRPVPAAAALTLTALTAVKVARRMPDADQ
                     PARAGALLSLAALGGTAEQLLRCATRHHWPLAVAAAAVSRRARYGLAVAALAEGLADHH
                     RSGSSLGPLTHTAIRRLDDLAYGWGVWQGALRRRTAAPLLPRIALPLRGKAAKHPWEPR
                     SLAPTSHQPATATVETW"
     gene            16251..18344
                     /gene="acs"
                     /locus_tag="N8I87_RS20610"
                     /old_locus_tag="N8I87_20610"
     CDS             16251..18344
                     /EC_number="6.2.1.1"
                     /GO_function="GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (90-529). E-value: 4.9e-100.
                     Score: 326.9. Matches aSDomain:
                     nrpspksdomains_N8I87_RS20610_AMP-binding.1"
                     /codon_start=1
                     /gene="acs"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473275.1"
                     /locus_tag="N8I87_RS20610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20610"
                     /product="acetate--CoA ligase"
                     /protein_id="WP_263216564.1"
                     /sec_met_domain="AMP-binding (E-value: 3e-111, bitscore:
                     370.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPRSELRPAPGSPPAGGGTSHALEAVGEAEAVSGLYEEAATDRP
                     AFWARQAEHLHWETGWSAVLDWTDAPFARWFTGGRLNVAYNCVDRHVEAGHGDQVAIHW
                     EGEPGDTRTITYAELRREVCKAANALLSLGLSAGDRVAIQLPMIPEAVFAMLACARLGL
                     PHSVVFGGFSPAALQSRIEDAEARLLITSDGQYRRGRAEPMKAHTDEATRHAPSVEHVL
                     VVRRTGTDVPWTEGRDLWWHDVVDAQPDEHRPESFDAEHPLFILYTSGTTGKPKGILHT
                     SGGYLTQAAYTHRTAFGLDPDTDVYWCTADIGWITGHSYIVYGPLANRATSVMYEGTPN
                     TPHEGRHFEIIERYGVSVYYTAPTLIRTFMKWGEQLPARYDLSSIRVLGSVGEPINPEA
                     WRWYRTHIGGHRAPVVDTWWQTETGAVMIAPLPGAAPAKPGSAQQPVPGISAKVVDDQG
                     EEVGRGESGYLVLDEPWPAMLRGIWGDDERYRETYWARFAGQGYYFAGDGARYDEDGDI
                     WLLGRVDDVMNVSGHRISTAEVESALVSHPAVAEAAVVGAADATTGQAIVAFVILRGDT
                     VRAEGSEGSEGSEGSEGSDGSEGSEGSEGSEGSEGSEGGKGSEGDITPALREHVAQEIG
                     PIAKPRQIIVVPELPKTRSGKIMRRLLRDIAENREVGDVTTLADASVMDLINAGLTSRR
                     PSA"
     aSDomain        16521..17837
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N8I87_RS20610_AMP-binding.1"
                     /evalue="4.90E-100"
                     /label="N8I87_RS20610_AMP-binding.1"
                     /locus_tag="N8I87_RS20610"
                     /protein_end="529"
                     /protein_start="90"
                     /score="326.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="HVEAGHGDQVAIHWEGEPGDTRTITYAELRREVCKAANALLSLGL
                     SAGDRVAIQLPMIPEAVFAMLACARLGLPHSVVFGGFSPAALQSRIEDAEARLLITSDG
                     QYRRGRAEPMKAHTDEATRHAPSVEHVLVVRRTGTDVPWTEGRDLWWHDVVDAQPDEHR
                     PESFDAEHPLFILYTSGTTGKPKGILHTSGGYLTQAAYTHRTAFGLDPDTDVYWCTADI
                     GWITGHSYIVYGPLANRATSVMYEGTPNTPHEGRHFEIIERYGVSVYYTAPTLIRTFMK
                     WGEQLPARYDLSSIRVLGSVGEPINPEAWRWYRTHIGGHRAPVVDTWWQTETGAVMIAP
                     LPGAAPAKPGSAQQPVPGISAKVVDDQGEEVGRGESGYLVLDEPWPAMLRGIWGDDERY
                     RETYWARFAGQGYYFAGDGARYDEDGDIWLLGRVDDVMNV"
     CDS_motif       17040..17093
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N8I87_RS20610_0001"
                     /evalue="4.80E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="N8I87_RS20610"
                     /protein_end="281"
                     /protein_start="263"
                     /score="22.2"
                     /tool="antismash"
                     /translation="FILYTSGTTGKPKGILHT"
     CDS_motif       17808..17870
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N8I87_RS20610_0002"
                     /evalue="2.20E-05"
                     /label="NRPS-A_a8"
                     /locus_tag="N8I87_RS20610"
                     /protein_end="540"
                     /protein_start="519"
                     /score="17.1"
                     /tool="antismash"
                     /translation="LGRVDDVMNVSGHRISTAEVE"
     misc_feature    complement(18377..18395)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.73"
                     /tool="antismash"
     gene            18378..18938
                     /locus_tag="N8I87_RS20615"
                     /old_locus_tag="N8I87_20615"
     CDS             18378..18938
                     /EC_number="2.7.4.3"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA]"
                     /GO_process="GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473933.1"
                     /locus_tag="N8I87_RS20615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20615"
                     /product="adenylate kinase"
                     /protein_id="WP_263210656.1"
                     /transl_table=11
                     /translation="MRLVLIGPPGAGKGTQATLLGRRFGIPHISTGDLFRENVSHGTPL
                     GQEARRYLDAGELVPDHVTGAMVRRRLEEPDARRGFLLDGFPRTVEQAHALTTILARTG
                     AVLDAVVEFDAPEHIVVERLRGRGRSDDTEEVIRHRQQVYRRQTAPLLAHYTDIVLTVH
                     AVGTVEEITDRALEALADLPGAA"
     gene            18993..19970
                     /locus_tag="N8I87_RS20620"
                     /old_locus_tag="N8I87_20620"
     CDS             18993..19970
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998790.1"
                     /locus_tag="N8I87_RS20620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20620"
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="WP_263210657.1"
                     /transl_table=11
                     /translation="MRVSVVDAGSNTVRLVVADAEGGVPLPVHTVKWRLRLSERVEPDG
                     TIGEEAVAQLVKAVAAAGGAAEQWRAPGPLAFATTVVRNAPNRQEVLDRVGSETGIRMC
                     TLPGETEARLTFLAARRWMGWQAGPLALLDIGGGSLEVAFGRGRLPDFAASLPLGADRL
                     TREFLAAQDPPAQAELKALRRRVRHELRDVAARVRWEGPRTAAVTSRTFQQLARLCGTA
                     PGRHGPFIQRDMLRSDLRQAIGALAALSAAERAQLPGISAPRAAQSLAGALVGHTAMKL
                     MGLHRVAVCPWAIREGVLLRYIEDGADWWADATSEIGTDAGCST"
     misc_feature    19536..19538
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(20050..20742)
                     /locus_tag="N8I87_RS20625"
                     /old_locus_tag="N8I87_20625"
     CDS             complement(20050..20742)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020119476.1"
                     /locus_tag="N8I87_RS20625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20625"
                     /product="DUF5324 family protein"
                     /protein_id="WP_263210659.1"
                     /transl_table=11
                     /translation="MTRIDSVRAATGSAKDSVLHAAEVVAPYADTAKERASYYAQEARV
                     RLAPVVSQAAEQARVQYDAHLAPRLEQALAHVPPKVDQAAQDAAVLTRKAARQAADYSR
                     PRIEQAIAAAGPMRDEATARGVAALAALRGQVSPEEIQKLVRRHERREKAGRVAKVLVV
                     LGTVAAGAFVAWKWWDKQTNPDWLVEPPAATEVPESGRLSSVDGSLDPEVAAKEAEEQA
                     AKDDKHRH"
     misc_feature    complement(20950..20965)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.64"
                     /tool="antismash"
     gene            21076..21609
                     /locus_tag="N8I87_RS20630"
                     /old_locus_tag="N8I87_20630"
     CDS             21076..21609
                     /EC_number="5.2.1.8"
                     /GO_function="GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /GO_process="GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009996872.1"
                     /locus_tag="N8I87_RS20630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20630"
                     /product="peptidylprolyl isomerase"
                     /protein_id="WP_263210661.1"
                     /transl_table=11
                     /translation="MAEQLYATLKTNHGDIEIRLFPNHAPKTVRNFVELAQGEREWTHP
                     ETGVKSTDRLYDGTVFHRVISGFMIQGGDPLGTGIGGPGYEFEDEFHPDLSFNKPYLLA
                     MANAGPATNGSQFFITVGPTIWLNRKHTIFGEVTDPAGQKVVDAIANVPTTRPNDRPVN
                     DVVIESVVIEKREG"
     gene            21748..22635
                     /locus_tag="N8I87_RS20635"
                     /old_locus_tag="N8I87_20635"
     CDS             21748..22635
                     /EC_number="3.4.21.-"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002231.1"
                     /locus_tag="N8I87_RS20635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_20635"
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="WP_263210662.1"
                     /transl_table=11
                     /translation="MDQAAGSAQDAQRVPVCYRHPDRETGVHCTRCERPICPECMVSAS
                     VGFQCPECVRTGSGTGHSPAASRPRTVAGGTVTADPRLVTKVLIGLNLAFFLVQSAVGD
                     SFTDRFDLIGQAYVPLLGSVQGIAHGQSYRLLTAMFLHGSYIHILFNMLSLWWIGGPLE
                     AALGRARYIALYFVSGLAGSALSYLLAAPNQPSLGASGAIFGLFGATAVLMRRLNYDMR
                     PVIALLVINLIFTFSWSGIAWQAHIGGLVAGLVCGYAMVHAPRRHRNLIQYGACVLFLV
                     AVVVLTLLRTAQLT"
ORIGIN
        1 cgctccggtg agaagccgtc cggttcgagg gcgagcaggg tcaggatctc cagctgccgg
       61 gggcgcagcg cgaggggccg ctcctcgcgg acggcctggt cggtccccag gcaggtcaga
      121 cgcaccgggc ccgggttgtc gcgggtccgg cggggcggct cggtgcgcag ccgggcctcg
      181 atgacggaca ccagcgagcg caccgtggac atggccagcg ggttcgagcg gtcccaggtg
      241 gtggacatgt ccaggacgcc caggacgcgt ccgtccgggc cgtggacggg ggcgcagtag
      301 cagacccagc cgtgcagtgc cgtcaccagg tgctcggcgg agaagatgga gctggggcgg
      361 ccggtgcgca gggcgaggga gagggcgttg gtgcccatgg cctgctcgtc ccaccgtccg
      421 ccgggcgcga agttgacgcg ctcggcgcgg cggcgcatgg tccggccgcc gcaggtccac
      481 aggatggtcc cggactcgtc ggtgaccgcg gtgacgaatc cggcgtcgtc ggcgatgccg
      541 cgcagctcgt cggcgaggtc gtcgaccggc cggcgcagcg gggagtcggt ccagcggtga
      601 tgcaccgtgt cgccgtcgct gacgggggcg ctgtcccggc cggggtccac gctgccgagc
      661 gagcgcgccc aggactcggt cacctcgtgg cgcaacgcgt cggcgccgcc gtccgccgcg
      721 ggtccggtgg tcttcagccg cggcaccagg cggaaccact caccctccag cgtggccttg
      781 cggcgctgga ggtcactgcg ctgtgacatc tgcatttccc ctgccgtcga acggggacat
      841 tcggctattg tccgcgagaa cttcgtcggc gcacctgacc tgtcgggccg gtttccctgt
      901 ccgatcggac aggtgccgta cccggaccgg ggtgcctagc ttggtcttcg gtccgtagaa
      961 agttttatcc gtcaatcagg cggtaaaatc aaggttgcgt ccgcgttgcg ggccttgctc
     1021 atgggaggtc gcatgacgct catgaccggc gccgtgacga ccggtggcac cgatccgagc
     1081 gccgactgga gccgtgacga gcgccagggc gagctgctgg ggccgcagga gacgagcagt
     1141 gacgccgtcc gcaggtcggc ccggctggcc aagttccacg tgcccgaggt cgtgttcggg
     1201 cctggctcgc tgaccgagct gggacactgt gcgctgcgcc tcggcggccg tcgcccgttc
     1261 ctggtcaccg acccgggagt gctggaggcg ggctggacgg acgaggcggt cggccatctc
     1321 aggcgggtcg gcctgcgtcc cgtcgtctgg cacgacgtga cccccaatcc caaggaccac
     1381 gaggtccagg ccgccttcga gaggtacgcg cagagcggct gcgacgtgat cgtcggggtg
     1441 ggcggcggct cggtcatcga cgccgccaag ggcgtggcga tcctgtccgg caacggcggg
     1501 cacattctcg actacgaggg tgtggaccag gtcgtacagc ccatcccgcc caccgtgatg
     1561 gtgccgtcca cgtcgggcac cggggcggac gtctcgcagt tcgtcatcat caccgacacc
     1621 acccagcaga tcaagatcag catcgtcagc cgcaccctgg tcccggacat ctcggtgatc
     1681 gacccacgcc tgctgaccac catgccggac tggctgaacg ccgcgaccgg gctggacgcc
     1741 ctgacccacg ccgtcgaggc gttcgtgtcc cgggcgcaca acccgctgac cgacatccac
     1801 gcgctgcacg cggtggagct gatcaccggc aacctggtac ggacccaggt gcagccgaag
     1861 aacttcggtg cccggctggc catggcccag gcctccctgg aggcgggcat ggccttcacc
     1921 aacgcgatcc tcggtgcgac gcacgccatg agccaccagg tcggcggact gctggacgcg
     1981 ccgcacggcg tggtcaacgg tgtcctgctg ccgcacgtca tccggttcaa cgccgcggcg
     2041 tggccggagc ggttcgtccg gctcggcgcg gcggccggcc tgcccaccga cggcgttccg
     2101 ccgtacgagg tggccgagcg gctcgcggac ctggtgcgcg ccctcgcgga cgacctcggc
     2161 gtcccgaaga gcctggcgag cctgggggtc gcggaacacg acgtgcccgt gctggcccgc
     2221 accacgctga gggacgcctg catggccacc aaccccaggg acgtcgacgt gcgggacgtc
     2281 gagcggctgt tccgcgaagc cctgtgaggc gatcccggtg agcgtccccg accggtaccc
     2341 gcagaccgcc gacctgggcg tcctcaccgg gctgcggtcc ggcaaacggt cgtactaccc
     2401 ggcctacgtg cgctccgccg agcggctgga gcggacagtg gaggcactcg acgggatctc
     2461 ccgcgccctg gtgcgcaccg ccgagggccc gcgtgccctg gtcgaggcgg tcgtccggac
     2521 ggcggccgag catctgcagg cccgctggct gctgctggcc gtggcggacg gagcgctgcg
     2581 cgcggcccgg ccccgcttcc tgctgtacgc cgacgacctg ctgatcgaca acgagagcag
     2641 ggtgcccgcc gaggtgcgtg agcatctggc gctgctgcgc agtcacccct gggaggccgg
     2701 cacgccggac cggggccggg actgggtgcg cgcgccgatg accctggacg acgagccggt
     2761 cggcgggatc gcggcggcgc ccggccccgg tgtcgaggtg gccgacaccg acctggcgat
     2821 cctgcgggtc ctggccaacc aggccgccgt ggcgctgcac cacaccttcc tcttccacgc
     2881 ggcgaccacc ctgcgcggcc gggccgagga actgtccgag gcggccgagc ggcaggcccg
     2941 cgacctggcc gcgcgcagcg ccgagctggc cgagacgcag gcacggctgc tggaggcgtt
     3001 gcagcggcag gcactggacg acgaacggcg ccgtatcgcg cgcgaactgc acgacagcgt
     3061 ctcccagtac gtgctcagcg cgggcatgac cgtcgaggtg tgccgggcgg agctggcggc
     3121 gctgggcggg ccggccgcgg acatcgccga acggctggcc ggcgccaagg acctcaaccg
     3181 gcaggccgtg gagcggctgc gggccgcgat ctacgccctt cagcacacgt cgcaggagcc
     3241 cacggggccg ctgccggtga tgctggagcg gctgtccacg gtgcatctca ccacggacct
     3301 cgacgtacgg gtccaggtga cgggcagccc ggtgccgctg ccgcccgagg ccgaccagtc
     3361 gatcctccgg ctgaccggcg aggcgctgtt caactccgcc acgcacggca aggcgggccg
     3421 ggcactggtc cggctgcggt atctgcccga ggccgtcgtg ctgacggtgt ccgacgacgg
     3481 ctgcggcgat ccggcccagc tgcgccgcgc gctgcggctg tccagggccg ccgatctggg
     3541 cggccgacac cgcggcctgg cgaacatggt cacccgggcg gaggagttcg gcggccggct
     3601 gtccatccgc cggtcgggca tgggaggcgt cctgctgcgg gtcggcatcc cgctgccggt
     3661 gaagcactgt ccaggaggca gcagatgacc actcagcagt cggccgcggc gcccacgcag
     3721 gcccagcgga tcgtcctggt cgacgaccac gcgatcgtcc ggcacggcct caggtcgatc
     3781 ctggaacgcg agagcgacct ggaggtggtg ggcgaggcga gcacggccgc cgaagccgcc
     3841 gccgtcgtgg agcggaccga cccgtcgatc gtgctgctcg acctgaagct gtccacggcg
     3901 gccgacaccg agggcttgga cctgtgcgcc cggctgaccc agcggtaccc ggatctggcg
     3961 gtgctggtgc tcaccacctt cctcgacgac tccctggtgg tgcaggcgat ccagcatggc
     4021 gcgcgcggtt acgtcgtcaa ggacgtcgac accaccgagc tgctgcgctc catccgcgcg
     4081 gtcgcccgca acgagagcgc cttcgactcg cacgccgccg cggcgatggt gcggtcgatg
     4141 cgcgtgcagc ccgaccggcc gaagttcacc gaccgcgagc tcaaggtgct ccggctgctc
     4201 gcccacgggc tgagcaaccg ggagatcggc gccgagctgt acatctcgga gaccacggtg
     4261 aagttccacg tccgcaacat catgcggaag acggaggccg gaagccgggc cgaagtcgtg
     4321 tacgaggcga ccaagatggg cgtgatctga gaacgggaga cgagcgcggt cagctcgttt
     4381 cgagggtcag ccagccgccg tggtgcgaag tgacggagaa acggtggccg gtgcggcggc
     4441 ccagtgcggt gagcccggcc gggtcgaaac accgcacatg cccgtaggcg gcggtgggtt
     4501 cgtcctcgta cggcacggcc accaccaccc gctgcccggc cagcctcagc gcctcccgta
     4561 cgacggtgtc gccgtgcccg ggatcgaggt gctccaggac gtggatgacc gtgacggtgt
     4621 ccaccgagcg gtccggcagg gggacctgtg ccgcgtcgca ggccagggtg tcgagggcga
     4681 ggccgcgtgc ccccgccacg gcggccagca ggcgcatggt gccgcgggag agatccgacg
     4741 cgatcacagt gtgcccctgc tgctctgcca tcagcagggg caggaagccg aagcaggagc
     4801 ccatgtccag caccgtgccc ggggccacca ggtccagggt gcggcggtag acgggggcga
     4861 acccggcgat cgacgagtga tcggccggct tcggtgcccg ccaggactcc cggatgcggg
     4921 ccagtgtgtt gtcgtagaac gtggtccagg cgggcagcgc tccgtccacg caggagcgca
     4981 ccaccccggt gaagacccgc tcgaagatgt cgctgccgga cagccagccg ggggcgaaca
     5041 gctcctcggc gagcaggccg gccagatcgt tgtccaggtg ctcggggcgc agcgagtgac
     5101 ccagctcgac ccggcggccc cggcggcgca gggtgaagtg ctcggtgcgg acgatgggcc
     5161 gtggtgtggc gtcgtcgggg cgagggctgt cgcggatcac gcggaccagg tggtcggcgt
     5221 acgcgccgga cgggcacggc gccagggggt cgatgcggcg cgcggcgatc gcgctgcgca
     5281 ggctgggcag gctcatcgtc cggtcacctc cgtggccatg cggcgggcgg tgcgttcgag
     5341 accggcgagg tcggcgacca cctggacgcg ggcgcagtac tcggcgtaga gcggcaggtc
     5401 gcaggagccg aggccccagg agaaccgggg ttccggggtc agccagatcg tctcgcgcac
     5461 cctgcgggtg atctcctcga aggcggccag cccggacggg ttgccgttgc cgcgtccgtc
     5521 gcccaggacc agcagggtgg tacggcggtt cagcgcgggg gcgtgttcgg cgagcagcag
     5581 ctcgaacgtc cggccgtagt cggagttcgc gtccacgtcg agcaggccgc cggccgggag
     5641 gccgtcgaag accaggccca gcgcccgctc cagggggtgc tcggtgaaca gctcggtgat
     5701 ctcggtcggt tcgtccacga aggcgaagga gcggacctgt ccgaacaggg actgcagccc
     5761 gtgcaccagg tgcagggtga agcgggcggt ggcccgcacg gacagggaga catcggtgag
     5821 gatcaccagg cgggggcggt cctcggcgcg ggtgacggtg accgggcgga acggcacccc
     5881 gtcgtagcgc atgttgcggc gcatggtgcg ggccggatgc acgcgcccgc gtggcgtggc
     5941 gcgcttccgg ctggtcaggg cgccgtgcag ggagcggccg acgcggcgca acgcctcctc
     6001 cagctgggtg cgttcgtgct cgtcgacggc ttccgcgcgg gcacgttcgg cgagctgagc
     6061 cgactccacg gccaccgact ggagttcggc gaggcggtcc aggtggcgcc tgagcagttc
     6121 gggcaggttg tcgatgacgc ctgcgggccc tcgccggagc gcgccgggtc cggggtcgtc
     6181 ggggtcgtcg gaggtgtcgt cggcgagcca gccgagcagg gcgtcctgct gggccacggt
     6241 cagcgtcgtg tcgatcgggt gtccggccgc cgccgcgagc tgcccgggga tgccggcgcc
     6301 gtgcagccgc tccgtctcca actggacgga cgggccgcgt gtggcgtcgg cggcgccgtc
     6361 cttggacagc acgatctcct cggtcatcgc ggccaggtcg agcttgttgg cctcctggtg
     6421 caggttgtag cgctgggcca ggtcctgctg gtcgaagtag tcgcggatgt cggccggctt
     6481 gccgtgactg tggccctgct gcggggtctc ggacggctcg tcggagacgg tgtacgactc
     6541 cagttcgccg gtgtcggtca gatcgtcgtg ctcatggctg tgaccgtgcc cgtcggagtc
     6601 ctggtggacc gggcggagcg agaagaacgc ggcgaacagc tcgtcgaaca gctcgtcgtc
     6661 gcggcggtcc ttcaccagcg tcaggcgcag ggcctcgcgc agtgtctcgc gcctgccgag
     6721 cacgcccggc tgggcggccg cgcgcatggc gtccatggcc tccgacacgg acacgcgcag
     6781 cccgaacagc cgtagaacgc ggacgaatcg gtgcaccccg gcttccattg cggcctcccg
     6841 tgtcctgttg gtgctgttgg tcctgttggt tctgtcgtcc tgggtcagag agcgcggcgg
     6901 cgggcgatgg cggcgaaggc gcgggcgccc tggccggcgc cgggtgtggt gcggcgcgtc
     6961 gggccggggc cgggtgcgcc gtagtagccg tcgtcgtggc ggccctgctg gtccatggcc
     7021 gcccggaccg cccgcccgtc cacctcgtgc tcgtggtcgt ggtcgtggtg gtgcccgttg
     7081 tgatggtggt ggccgtgccc gtcgtgccga tggcccggct cgggcagggt gcggttgggg
     7141 tcgacgaggc ggggcaggac gtccagggca cggcccaggt cgcgttcgta cttgaggacc
     7201 acgttggcgg tgtcggcgag cacctcggga tccagttcct cgacgcccag cacggcgagg
     7261 gtgcgggccc agtcgaccgt ctcggagatg ctggggttct tgcgcagttg cagctcgcgc
     7321 aggccccgta ccacctcgac cagatgacgg gcggtggcct cggacagccc ggtgtccttg
     7381 gcgcgcagga tctccagctc ccgctcggcg tccgggtagt cgaggaagag atgcagacag
     7441 cggcgcttga gcgcggccga caggtcacgg gtgttgttcg aggtcaggac gacgtacggc
     7501 gggaccttcg cggtgtacgt gccgatctcg gggaccgaca cctggtactc ggccaggcac
     7561 tccaggagta cggcctccag cgcctcgtcg gcccggtcga cctcgtcgat cagcagcacc
     7621 gccggctcct cgccgcggac cgcctccagc agcgggcgcg cggcgaggaa ccggtcggag
     7681 aagaacacgc tgtcctgttc ctcgatccgc tggacggccg aggccagatc gggcgcgtcg
     7741 gcggtcaggt cgccgatctt gtcccggagc atctgggtgt acagcagctg tttgccgtag
     7801 tcccactcgt acagggcgcg gctctcgtcc tggccctcgt agcactgcag ccgcagcagg
     7861 cggcgtccgg tggcggcggc cagcgccttg gccagctcgg tcttgccgac gccggccggg
     7921 ccctccagga gtaccggctt gtcgagtctg gtcagcagga acacggtcgt ggcgagccgg
     7981 cggtcggcga tgtagccgtg ggcggcgaag cgttcggtga tgtcctcgac cgaggcgaac
     8041 tcggcggggc gggcgggtgt ggcgggcacg ggtcctccat gaggtgctgt tcggccgggg
     8101 agggcgacgg ggcgggggac gacgagggtg gtccgtcccc cgccccggtc cggaagggct
     8161 cggtcgtggc gggtgttcag ccgagcagct cgtccaggtc gccgttcatg cagtggtcga
     8221 cgaccggccg gacggtgtcg aacgtgcact ccttggcgtt gcccggggtg caggcgtcgc
     8281 cgaggacgtg gttcgtgatg cggtcgacat cggcggcgtc gcccttgatc accttggcct
     8341 gctcgtagta cttggtcggg ccctggccca ggcggctctt ggagtaggtg tcctgctgga
     8401 cgtccgtgaa cttctccggg atgcccacgt cacgcagcag ccggatgccc gcggcgagcg
     8461 cggcgtcggc ggcctggacc ttggtcatgc cgtgggtgtc cacgcccatc gcctcggcga
     8521 tgtccgcgaa ccgctcgtac tgcgccggca tgttgaacgc ccacacgcgg ggcagcgcga
     8581 tggcgttgtt cagcccgtgg tgggtgtcgt agcacgcgct gaccgcgtgc gagatggagt
     8641 ggatgatgcc caggccacca gagttgaacg cctgcgccgc gatgtactgc gcgtacatca
     8701 tgccctcgcg cccggccagg tccgtgccgt tccacaccgc ctgccgcaga tgccgggccg
     8761 tgagccggat ggcgtgcagc gcgttgccca gcgagggctg gaagttgagc cgggagacgt
     8821 agggctcgga ggcgtgcgcg aggacgtcga aaccacactg cgcggtgtag tcgagcgggc
     8881 agtcgtagta cagggtcggg tcgtcgatgg cgagcgaggc caccgaggcg tcgtcgaagg
     8941 cgacgtactt gtgcggattg tccgggtcgg tggtggtgtc ggtgatgacg tacgcccagg
     9001 aggtctccga gcccgtgccg gccgtcgtcg agaccgcgat gtgcggcggg ttcttcgggt
     9061 tctcggactt gttgaagccc tcgaagtcgt tgacgttgcg gccgtcgtgg gcgacggaga
     9121 tacgggcgcc cttgcacgcg tcgtgcgacg agccgccgcc gatcgacacg aacgagtcgc
     9181 acttgttctc ctggtacagc gccacggcgt ccatgacgtt gtagtccttg gggttcgact
     9241 cgaccttgtc gtacaccacg acctcgaggc cgtggtactt cagcgactcg acgatcttgt
     9301 gcacggtgtc gctgccgcgg agacccgtgg tcatcaccag ggtgcgcctg aacccgagct
     9361 tcttggcctc cgggccgatc agctcgtggg ctccggggcc catcaacgcc cgcgggaagg
     9421 gatggaattc cttgatcggg aacggcttga gaagttcttc tacctgcatg atcgcgactg
     9481 tcctctccgc gcggtggagc cgcgcgtgct gcgtcgactg ctcccagggc ccgccggcgg
     9541 gcagaggtcc gggtggagca cggacgtgcc gaacgaatgc ggcgtgcatc accctccggg
     9601 accgcgtggc ggtgcgatac cggccgctcg gacggcaggc gggcacggac ggaggggatg
     9661 tgccctgtcc gatcggacgg ggtgagcgcc gctgacctgc ggcgcaacgg gcacacctgt
     9721 ccgaccggac agggtgaggg cggccgaaag cagggcgaga cgcgaaggcg accggcctag
     9781 cgtcgagtgg tgtccgggcc gcgtgccacg ccacgcggcc cggctcccct cactttcccg
     9841 cccctcacac caggaggact gccatgagtg ccgtacagga cgacaccctc gtcgccgagc
     9901 aggacgacac cgtcaacgcc gacgacgtca tcgaggagat cctcgtcgag gaggtctcca
     9961 tcgacggcat gtgcggcgtc tactaggccg gccggtcgtc atgccgtccc ccttcgacgc
    10021 ggaccggccc taccggctga acccctgcgt cgcgctgcgc cccgagccgt tcggggcgct
    10081 cgcctaccac ttcggcaacc gtcggctgtc gttcctgaag gcgcccgaac tggtcgagct
    10141 ggtacgggag ctgggccggc acgccagcgc ccgggaggcg ctcgccggtg tgcccgaagc
    10201 gcgccgggac accttccggc gcgcgctggc ctcgctggcc gccgccgaca tgatcatccc
    10261 caccggagcg gagccgcagc cgtgactgtc acccaagaac cgtccgcccc ggtccccgcc
    10321 ctcgtcgagc agttcaaggg cggcctgcac gcccccatct gcatgacctg ggaatggacg
    10381 tacgcgtgca acctctcctg tgtgcactgc ctgtcctcct ccggacggcg cgacccggcg
    10441 gagctgtcca cggacgagat caagtccgtg ctcgacgagc tccgacgcat gcaggtcttc
    10501 tacgtcaacg tcggcggcgg cgagcccacc gtccgccccg acttctggga actgctggac
    10561 tacgcgatcg cgcaccaggt gggggtgaag ttctccacca acgggctgcg gctgaccccg
    10621 gaccgggccc gccgcctcgc ggccaccgac tacgtcgacg tccagatctc cctcgacggc
    10681 gccacccgcg agatcaacga cgcggtgcgc ggccggggtt cgtacgacat ggccgtgcgg
    10741 gcgatggaga acctcgccgc ggccggcttc cgggacttca agatctccgt ggtgatgacc
    10801 cgtcagaaca tcccgcaact ggacgacttc gcgtccctgg ccgaccggta cggcgcccag
    10861 ctgcgcatca cccggctgcg gccgtccggg cggggcgccg acgtctggga cgaactgcac
    10921 cccaccgcac agcagcagcg cgagctgtac acctggctcg tcggccacgg cgagaaggtg
    10981 ctcaccggtg actccttctt ccacctcaac gcgctcggct cgcagccgct gcccggactg
    11041 aacctgtgcg gagccggacg cgtggtctgc ctgatcgacc cggtgggtga cgtgtacgcc
    11101 tgcccgttcg ccatccacga cgccttcaag gcgggcaacg tgcgtgcgcc gggcgggttc
    11161 gaacgggtgt ggcgcgactc cgcgctcttc accgacctgc gccggcccgc ctccggcggc
    11221 gcctgcgcga gctgtccggc gtacgacgcc tgccagggcg gctgcatggc cgcgaagttc
    11281 ttcacgggcc tgccgctgga cggcccggac ccggagtgcg tcatgggcca cggcgccacc
    11341 gccctcaccg ccaccgaacg ctccgcgctt ccgccccagg gcaacgacca ctcccaccgc
    11401 accgtgcccc tgggcatgcc cgccgtccgg acccggcggc cggaccgctt ctgcgacgag
    11461 agcccgctgg ccggcgccga cgccgtacac cacacgaagg gaacgtcatg agcaagtgga
    11521 tcgagacggt cgccgaggcc cagaggctgg cgaagaagcg gctgccgaag tccgtctaca
    11581 gcgcgctgct cgccggctcc gagaagggcg tctcctacga cgacaacacc accgccttcg
    11641 gtcagctggg cttcgccccg cacgtggccg gactgtcggc caagcgcgac ctggcgacga
    11701 ccgtcatggg acagcagatc gccctcccgg tgatcatctc cccgaccggc gtgcaggccg
    11761 tacacccgga cggagaggtg gccgtcgccc gcgccgccgc gtcgcgcggc accgcgatcg
    11821 ggctcagctc cttcgccagc aagccgctgc ccgacgtcgt ggcggccaat ccgcagacgt
    11881 tcttccagat gtactggatg ggctccaagg acgagatgca gcgccggatc gagtacgccc
    11941 gccaggccgg tgccaagggg ctcgtcgtca ccctcgactg gtccttctcc tacggccgcg
    12001 actggggcag cccgaagatc cccgagcggc tgaacctcaa ggccatggcg cagttcgcac
    12061 cggagacgct ggcccgcccg cgctggctgt gggacttcgt gaagtcccgc agcctgccgg
    12121 acctgtcgac cccgaacctc gccgagcccg gcaaggacgc gcccacgttc ttcggcgcct
    12181 acggcgcctg gctgcagacc ccgccgccct cctgggacga cgtgcgctgg ctgcgcgagc
    12241 agtgggacgg gccgttcctg ctcaagggcg tctgccgggt ggacgacgcc ctgcgcgcgg
    12301 tcgacgcggg cgtcaccgcg atctccgtgt ccaaccacgg cggcaacaac ctcgacgcca
    12361 ccccggcacc gatccgcgcc ctgcccgccg tcgcggacgc ggtcggcgac caggtcgaac
    12421 tgctgctgga cggcggcatc cggcgcggca gcgacgtggt caaggcgctg gccctgggcg
    12481 ccagggccgt gatgatcggc cgcgcctatc tgtggggcct ggcggcgggc ggccagaccg
    12541 gcgtggagaa cgtcctcgac atcctcagcc gcggcatcga ctccgccctg ctcggcctcg
    12601 gacgctcgtc cgtccatgaa ctgacccccg acgacctggt catcccgccc gggttcaccc
    12661 gcaccctggg cgcggtctga gatggccgcg ggccccgcgc ggctcacgga tctggcctgg
    12721 ccggacctca tggcccgccg ccccctggtg ctgctgccgc tgggctcctg cgagcagcac
    12781 ggcccacacc tccccctcga caccgacacg gcggtggccg ccgccgtcgc cgaacgggcc
    12841 gccgcccggc tcggcaaaga gctggacgtg ctggtcgcgc ccgcccagcc ctacggggcc
    12901 agcggtgagc acgagggctt ccccggcacg gtgtccatcg gccacgacgc gctgcgcctg
    12961 ctgctcaccg agaccggccg ctccctgctg cgctgggcgg ggcgcctgct ggtggtcaac
    13021 ggacacggtg gaaacatggt gagcctggcc gacgcggtga ccggtctgcg cggcgagagc
    13081 cgggacgcgg cctggtggcc gtgtgtgccg tcggggccgg cggcggacac ggacgcccat
    13141 gccggccgca ccgagacctc gctgatgctg cgcctgcggc cggccgccgt ccgtggcgag
    13201 cgggccgtgg ccggacccac cgaccccgtc catctgctga tggaccgcct ggtcgccgag
    13261 tccgttcgcg gggtcagccc ctccggcgtc ctcggagacc cggccggagc tcgcgcgagc
    13321 gagggcgaac ggctcctcga ccgcatggcg gaccgcctcg ccgccgacat ccgcgcctgg
    13381 cgggtcaccg gacagggcag gctcggcctg cccgaccggc agccggtcac cgcccggacg
    13441 ggggacgggc ggtgaccggc cgccgggtcg ccgtcgtcac cggcgccgca cggggcatcg
    13501 gcgccgcgac cgtacgccga ctggcccgcg agggctgggc cgtcgtcgcc gtcgaccggt
    13561 gcgaggacga ccccgaggtg ccctacccgc tcgccgaccc ggaacaactg gaccgcctga
    13621 cccatgagtt ccccctggtg cgcacggcca gggcggacgt ccgcgacgcg gccgcgctgc
    13681 gagccgccgt ggagcaggcc gagcgcgagt tcggcggggt cgacgccgcc gtggccgccg
    13741 cggcggtcat gctcgggggc ggccccgtgt gggagcacac cgacgcgcag tggcggacgc
    13801 tgctcgacgt ggacgtgatg ggcgtggcca acctcgcccg tgccgccgta cccgcgctgc
    13861 tgcgcgggcc gcagccccgc accggccggt tcgtcgccct cgcctcggcc gccgcgcacc
    13921 gcgggttgtg gggcctgggc gcctactgcg cggccaagca cgccgtggtc ggcctggtcc
    13981 gcggcctcgc ctgcgacctg cgcgacaccg gggtcaacgc ggtcgccgtg tcaccgggtt
    14041 ccacgcgcac ggacatgctg cgtgccacgg ccgacctcta cgaactgccc gaggtcgaca
    14101 agctcgccga gcagcagctg tccgggcggc ccctggagcc ggaggaggtg gccgcggtgg
    14161 tggcctgggc ctgctcggcc gactccttcg ccgtcaccgg ctccgtactc cacgcggacg
    14221 gagggttcac gacttgactt ctcccccagc tgaagcaggg ggattcaacc ctcacgggtt
    14281 gacctctctg cttcatcgac agttgccgac ccgaattaac ggcaagggtt ggagtgccaa
    14341 ccatcggtct tacaccagct ccacagatct gacatcccgc cagcccggcg gtaggcccag
    14401 ccagtttggc tttcgccgac cgggcgtgcc caagcctatc accgcggacc gtgggcgggc
    14461 ggtgattcga cctggcatcg attcaccgtt ccctgccctg ctccacagga gtttcattcc
    14521 tctcccggct caagccggga gtttcctcgg aggtatccga tgacacggtt cctgcgggac
    14581 gcggacccgt cccggtccgt cgggcgtgcg ggcgccttgc tgttcgccgg gcacgtgggg
    14641 tcgcccccgc ggtccgagcc gctgccgtcc ccgccgaagg accggctccc cgacgggttc
    14701 gcggtcgaac tcgccgccga cacccaccgc tcccgcgacg gccacctgat gctcggcggc
    14761 tccccgccga ggctgctgcg tctcggggcc ggtgccgtgc ggctgctgga ggacggtggg
    14821 ttcggggtgg cggacgccgc gagcgccgcg ctcgcccgcc ggctcctgga cgcgggcgtc
    14881 gcccaccccc gccccccact gcccccggtc cgcgacacca ccctggccat cccgatccgc
    14941 gaccgggccg accaactcga cgcactgctg gccgccctgc gcgccgaccc gcccacctcc
    15001 accctgcccg tcctggtcgt ggacgacggc tcccgggatc cggacgccct cgcccgcgtg
    15061 gcccggcggc acggcgcccg gctgctcgtg cacccgcgca acctcggccc ggccgccgca
    15121 cgcaacaccg cactgcgcca cgccgagacg gcgtacgtgg cgttctgcga ctccgacgtc
    15181 gtacccgaac cgggctggct cgcccccctg ctggcccact tcggcgaccc ggcggtggct
    15241 ctcgtcgcgc cccggatcgt cgccctgccg gtgcccgcgc cacgccggct cgaccgctac
    15301 gagcaggtcc ggtcgccgct ggacatgggc tcgcgggagg ggccggtcgt gccggtgtcg
    15361 atgctcggct acgtcccgag cgcgaccatc gtggtccgcc ggaccgccgt cggcgccggg
    15421 ttcgcccccg gcatgcgggt cggggaggac gtggacctgt gcatgcggct gcacgaggcg
    15481 ggctggcggc tgcgctacgt ccccgacgtg cgcgtgggcc accggcaccg caccgacctg
    15541 cgccgctggc tcgcccagcg ggccggatac ggcaccggtg ccgccgacct ggccctgcgc
    15601 caccccggcc gggtgccgcc gctgtacgcc gccccctggt cgctcgccgc ctgcgccctg
    15661 ctgctgcgcg gcaggccggt acccgcggcc gccgcgctga cgctgaccgc gctgaccgcc
    15721 gtgaaggtgg cccggcgcat gccggacgcc gaccagcccg cccgggccgg cgccctgctg
    15781 tcgctggccg cgctcggcgg taccgccgaa caactgctgc gctgtgccac ccggcaccac
    15841 tggcccctcg ccgtggcagc cgccgctgtc agccgccggg cacgctacgg cctcgccgtc
    15901 gccgctctcg ccgaggggct ggccgaccac caccgttccg gctcctcgct gggcccgctc
    15961 acgcacacgg cgatccgccg tctggacgac ctggcctacg gctggggcgt gtggcagggc
    16021 gcactgcgcc gccgtaccgc cgcgccgctg ctgccccgca tcgccctgcc cctgcgcggc
    16081 aaggccgcga agcacccctg ggagccccgc tccctcgcgc caacctcgca ccaacccgcc
    16141 acggccaccg tggagacctg gtgacggacg ggtcacccgg cctcccgcga tccgcccgtc
    16201 accccacccg tacccctgga ggaaccagca tgacgcctgg aggaaccaac atgacgccgc
    16261 gctccgaact ccgccccgca cccggcagcc cgcccgccgg tgggggcacc tcccacgccc
    16321 tggaggcagt gggggaggcc gaagcggtgt ccgggctgta cgaggaggcg gccaccgacc
    16381 gcccggcgtt ctgggcgcgg caggcggaac acctgcactg ggagaccggc tggtccgccg
    16441 tcctggactg gacggacgcg ccgttcgccc gctggttcac cggcgggagg ctgaacgtcg
    16501 cgtacaactg cgtcgaccgc cacgtcgagg ccggtcacgg cgaccaggtc gccatccact
    16561 gggagggcga gccgggcgac acccggacca tcacctacgc cgagctgagg cgcgaggtgt
    16621 gcaaggcggc caacgccctg ctctcgctgg gcctttcggc cggggaccgg gtcgccatcc
    16681 agctgccgat gatccccgag gcggtgttcg ccatgctggc gtgcgcccgg ctcgggctgc
    16741 cgcacagtgt cgtcttcggc gggttctcgc cggccgccct ccagtcacgg atcgaggacg
    16801 ccgaggcccg cctcctgatc acgtcggacg gccagtaccg ccggggccgg gccgagccga
    16861 tgaaggcgca caccgacgag gccacccggc acgcaccgag cgtcgaacac gtcctggtgg
    16921 tccggcgcac cggaaccgac gtgccctgga ccgagggccg cgacctgtgg tggcacgacg
    16981 tggtcgacgc ccagccggac gagcaccggc cggagtcctt cgacgccgag cacccgctgt
    17041 tcatcctcta cacctccggt acgaccggaa agccgaaggg catcctgcac acctccggcg
    17101 gctacctcac ccaggccgcg tacacccacc gcaccgcctt cggcctcgac ccggacaccg
    17161 acgtctactg gtgcaccgcc gacatcggct ggatcaccgg gcactcctac atcgtgtacg
    17221 gaccgctcgc caaccgcgcc acctcggtga tgtacgaggg cacgcccaac accccgcacg
    17281 aggggcggca cttcgagatc atcgagaggt acggggtatc ggtctactac accgccccca
    17341 ccctcatccg caccttcatg aagtggggcg agcagctccc ggcccgctac gacctctcgt
    17401 cgatccgcgt gctcggcagt gtcggcgagc cgatcaaccc ggaggcatgg cgctggtacc
    17461 gcacgcacat cggtggccac cgcgcgccgg tcgtggacac ctggtggcag accgagaccg
    17521 gcgcggtcat gatcgcaccc ctgcccggag cggcccccgc caagccgggc tccgcgcagc
    17581 agccggtgcc gggcatctcg gccaaggtgg tcgacgacca gggcgaggag gtcggccggg
    17641 gcgagagcgg ctacctggtg ctcgacgagc cctggccggc catgctgcgc ggcatctggg
    17701 gcgacgacga gcgctaccgc gagacctact gggcccggtt cgccggacag ggctactact
    17761 tcgccggcga cggcgcccgc tacgacgagg acggcgacat ctggctgctg ggccgggtcg
    17821 acgacgtcat gaacgtctcc ggtcatcgca tctccaccgc cgaggtcgag tcggcgctcg
    17881 tctcgcaccc cgcggtcgcc gaggccgcgg tggtgggggc ggcggacgcg acgacggggc
    17941 aggcgatcgt cgcgttcgtg atcctgcgcg gtgacacggt ccgggccgaa gggtccgaag
    18001 ggtccgaggg gtccgagggg tccgaggggt ccgacgggtc tgaagggtct gaagggtctg
    18061 aagggtctga agggtctgaa gggtctgaag ggggtaaggg gtccgaggga gacatcaccc
    18121 cggccctgcg cgagcacgtg gcccaggaga tcggccccat tgccaagccc cgccagatca
    18181 tcgtggtccc cgagctgccc aagacccggt ccgggaagat catgcggcgg ctgctgcgcg
    18241 acatcgccga gaaccgcgag gtcggcgacg tcacgacgct cgccgacgcc tcagtgatgg
    18301 acctcatcaa cgccgggctc acgagccgcc ggccctccgc ctgaaccgac ctgatcactg
    18361 gaaggcgcaa ccccctgatg cgactcgtcc tcatcggccc tcccggagcg ggcaagggca
    18421 cccaggccac cctgctcggc cgccggttcg gcatcccgca catctccacc ggcgacctgt
    18481 tccgggagaa cgtcagccac gggacaccac tcgggcagga ggcccggcgc tacctggacg
    18541 cgggcgaact cgtgcccgac cacgtcaccg gcgcgatggt ccgccgtcgc ctggaggaac
    18601 cggacgcccg gcgcggcttc ctcctcgacg gttttccccg caccgtcgag caggcgcacg
    18661 ccctcaccac catcctggcc aggaccggag cggtcctgga cgccgtcgtc gagttcgacg
    18721 cccccgagca catcgtggtg gaacgcctgc gcggccgtgg ccggtccgac gacaccgagg
    18781 aggtcatccg gcaccgccag caggtctacc ggcggcagac ggcaccgctc ctcgcccatt
    18841 acacggacat cgtgctgacc gtgcacgccg tcggaaccgt cgaggagatc accgaccggg
    18901 cactggaggc gctcgcggac ctgcccggag cggcgtgagc ggttcccctt cgtggtgggg
    18961 tcacggccgc tgagctgggt acgcgaaggg tcatgcgggt gagcgtggtg gacgcgggat
    19021 ccaatacggt ccgactggtg gtggccgacg ccgagggcgg tgttccgctg ccggtccaca
    19081 ccgtcaaatg gcgactgcgc ctgtccgaac gggtcgagcc cgacgggacg atcggcgagg
    19141 aggcggtggc gcagctcgtg aaggccgtcg ccgcggcggg cggcgcggcc gagcagtggc
    19201 gggcgccggg cccgttggcg ttcgcgacca cggtcgtgcg caacgccccc aaccgtcagg
    19261 aggtactgga ccgggtgggg tcggagacgg gcatccggat gtgcaccctg ccgggggaga
    19321 cggaggcacg gctgacgttc ctggcggccc gccgctggat gggctggcag gccggccccc
    19381 tcgcgctcct cgacatcggg ggcggctcgc tggaggtggc tttcgggcgc ggccgcctgc
    19441 ccgacttcgc cgcctccctc ccgctcggcg cggaccggct gacccgggag ttcctcgccg
    19501 cccaggaccc gcccgcccag gccgagttga aggcgttacg gcggagggtg cgccatgagc
    19561 tgcgggacgt cgcggcgcgg gtccgctggg agggcccgcg caccgccgcc gtcacctccc
    19621 gcaccttcca gcagctggcc cggctgtgcg gtaccgcccc cggacgccat ggccccttca
    19681 tccagcgcga catgctccgt agcgacctgc gccaggccat cggcgcgctc gccgccctct
    19741 ccgcggccga acgcgcccag ctccccggga tctccgcgcc ccgcgccgcc cagagcctgg
    19801 ccggagccct cgtcgggcac accgccatga aactgatggg cctgcacagg gtcgccgtct
    19861 gcccctgggc catccgtgag ggagtcctgc tccgctatat cgaggacggc gccgactggt
    19921 gggcggacgc caccagcgag atcggcaccg atgccggatg ttccacgtga aacatcgccg
    19981 tggggcacaa gaccctgagg tcctccgccc cacggcgacg ttcgcgacga cagatgtcgg
    20041 cggcgcagct cagtggcggt gcttgtcgtc cttcgccgcc tgctcctcgg cctccttggc
    20101 cgccacctcg gggtcgagac tgccgtccac cgacgacagg cggccggact cgggcacctc
    20161 cgtggccgcc ggtggttcca ccagccagtc ggggttggtc tgcttgtccc accacttcca
    20221 ggcgacgaag gcaccggccg ccacggtacc cagcacgacc agcaccttgg cgacacggcc
    20281 ggccttctcc cgccgctcgt gcctgcggac cagtttctgg atctcctccg gtgagacctg
    20341 accgcgcagc gcggccagcg cggcgacgcc ccgcgcggtg gcctcgtcac gcatcggccc
    20401 cgccgccgcg atggcctgct cgatcctcgg ccgcgaatag tcggcggcct gccgggcagc
    20461 cttgcgggtg aggacggccg cgtcctgcgc ggcctggtca accttcggcg gcacatgcgc
    20521 aagggcctgt tccaggcgcg gtgcgagatg ggcgtcgtac tggacgcggg cctgctcggc
    20581 ggcctgggac accacgggcg caagccgtac gcgcgcctcc tgcgcgtagt acgaggcacg
    20641 ctccttggcc gtgtcggcgt agggcgccac cacttccgcg gcgtgcagca cgctgtcctt
    20701 cgccgagccg gtcgcggcgc gcacgctgtc aatgcgggtc acggcttcct cctcctcggt
    20761 ggcgtacggt atttcgactt tccacacttt tacggatcat gcctgtcaag catctgcgcg
    20821 gcacgcgagg gagggcatac gggtgctgat gcactcgatc aagcgcaagc gagaatgaat
    20881 gacgaattct ggacaacagg agcttgtcgt cgacaatgcc acggatcgcc gccacgcgct
    20941 cccgcccaga ggggactcga cctgttttcc gcaagcgatt cgtgcggatg agcgcgcatc
    21001 gacctcggcc gggcgacgcg atggcccggc cgtgcaagga tcgggggatc acaggaagac
    21061 aacggaaggc agatcgtggc cgagcagctc tacgccaccc tcaagaccaa ccacggcgac
    21121 atcgagatcc ggctgttccc gaaccacgcg ccgaagaccg tcaggaactt cgtcgagctc
    21181 gcccagggtg agcgcgagtg gacccacccc gagaccggtg tgaagagcac ggaccggctc
    21241 tacgacggca cggtcttcca ccgggtgatc agcggcttca tgatccaggg cggtgacccg
    21301 ctcggcaccg gcatcggcgg tcccgggtac gagttcgagg acgagttcca cccggacctc
    21361 tccttcaaca agccctatct gctcgccatg gccaacgccg gcccggccac caacggctcg
    21421 cagttcttca tcaccgtcgg cccgaccatc tggctgaacc gcaagcacac catcttcggc
    21481 gaggtcaccg acccggccgg ccagaaggtc gtcgacgcca tcgccaacgt gcccaccacg
    21541 cgtcccaacg accggccggt caacgacgtc gtcatcgagt cggtcgtcat cgagaagcgc
    21601 gagggctgag ccgggtgtcg ctcggaccat cccggcctcg cggggcctgg gcggctcacc
    21661 ggtttctccc ggagggaacc aaacgccccg ctcgtccgta aggatgagcg gggcggtctt
    21721 attgcccacc acgaccgagg ggatctcatg gaccaggctg caggcagcgc gcaggacgcc
    21781 cagcgcgtgc ccgtgtgcta tcggcacccg gaccgcgaga ccggcgtcca ctgcacccga
    21841 tgcgagcgtc cgatctgccc cgagtgcatg gtcagcgcgt ccgtgggctt ccagtgcccc
    21901 gaatgcgtcc gcaccggctc gggcacgggg cactccccgg ccgcgtcccg gccccggacg
    21961 gtcgcgggag gcacggtcac cgccgaccca cggctggtca ccaaggtgct gatcggcctc
    22021 aatctggcct tcttcctggt ccagagtgcc gtgggcgaca gcttcaccga tcgcttcgac
    22081 ctgatcggcc aggcctacgt cccgcttctc ggctcggtcc agggcattgc gcacggccag
    22141 tcgtaccggc tgctgaccgc gatgttcctg cacggcagct atatccacat cctgttcaac
    22201 atgctcagcc tgtggtggat cggcggcccc ctggaagcgg ccctcggccg ggcccggtac
    22261 atcgcgctgt acttcgtctc ggggctggcc ggcagcgccc tcagctatct gctggccgca
    22321 cccaaccagc cctcgctggg cgcctccgga gcgatcttcg gcctcttcgg tgcgaccgcc
    22381 gtcctgatgc ggcggctcaa ctacgacatg cgcccggtca tcgccctgct ggtgatcaac
    22441 ctcatcttca cgttcagctg gagcggcatc gcctggcagg cccacatcgg cggcctggtc
    22501 gcgggcctcg tgtgcggcta cgccatggtc cacgctccac gtcggcaccg gaacctgatc
    22561 cagtacggcg cctgtgtgct gttcctggtc gcggtcgtgg tcctgacgct gctcaggacc
    22621 gcacagctca cctgataccg aaccgttgtc cacagcgtgt ggcggatctt gtgcacgctg
//
