LOCUS       NZ_CP106798            10405 bp    DNA     linear   CON 01-NOV-2023
DEFINITION  Streptomyces sp. HUAS 15-9 chromosome, complete genome.
ACCESSION   NZ_CP106798
VERSION     NZ_CP106798.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. HUAS 15-9
  ORGANISM  Streptomyces sp. HUAS 15-9
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9258818)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 169.8x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 03:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,774
            CDSs (total)                      :: 8,679
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 504
            CDSs (without protein)            :: 504
            Pseudo Genes (ambiguous residues) :: 0 of 504
            Pseudo Genes (frameshifted)       :: 260 of 504
            Pseudo Genes (incomplete)         :: 328 of 504
            Pseudo Genes (internal stop)      :: 29 of 504
            Pseudo Genes (multiple problems)  :: 107 of 504
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:51:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2069162
            Orig. end                         :: 2079567
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106798.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(100..903)
                     /locus_tag="N8I87_RS09450"
                     /old_locus_tag="N8I87_09450"
     CDS             complement(100..903)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381142.1"
                     /locus_tag="N8I87_RS09450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09450"
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="WP_317633452.1"
                     /transl_table=11
                     /translation="MSIRGTAEIQVRDLHKSFGDNQVLKGIDLEIAQGEVVCVIGPSGS
                     GKSTLLRCVNLLEEPTKGQVFVGGTEVTDPDVDIDAVRRRIGMVFQQFNLFPHLSVTEN
                     LTLPQRRVLRRDKATAARIAAENLARVGLTEKAEAYPSSLSGGQQQRVAIARSLAMGPE
                     VMLFDEPTSALDPELVGDVLAVMRMLADEGMTMMVVTHEMTFAREVADRVVFMDGGVIV
                     EDGPPAQVIGSPQHERTRHFLSRLLDPAMAEVEEETSDQVGKSEG"
     gene            complement(900..1733)
                     /locus_tag="N8I87_RS09455"
                     /old_locus_tag="N8I87_09455"
     CDS             complement(900..1733)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661368.1"
                     /locus_tag="N8I87_RS09455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09455"
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_263207296.1"
                     /transl_table=11
                     /translation="MTDTDAALQPKKKGLTRRQKRSLSRGVQYTVFAAAVIALAVTADW
                     GRLRNQFAQPDIAKQMFPDVITLALKNTVLYTVSGFVLAMALGLVVALMRLSSVGPYRW
                     VASVFIEVFRGLPALLIFVFIGVAVPLAFPGTEIPGGTYGKAAIGLGLIGAAYMAETFR
                     AGIQAVPKGQMEAARSLGFSPARAMVSIIIPQAFRIVLPPLTNEFIMLFKDSSLVLLLG
                     VTLEERELSKFGRDLASTTANSTPILVAGLCYLLVTIPLGFVARRLEANAQEAVK"
     gene            complement(1738..2592)
                     /locus_tag="N8I87_RS09460"
                     /old_locus_tag="N8I87_09460"
     CDS             complement(1738..2592)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="N8I87_RS09460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09460"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_263207298.1"
                     /transl_table=11
                     /translation="MNTLFGRRARVLAATTATVGLVLVAGCSSSDNGGGAKKTAVGGVE
                     LAKAGQLTTCTHLPYPPFQSEIDGKVQGFDVSLIDLVAKDLGVKQTILDTPFENFKTGA
                     FLNSGQCDVAAAGMTITAERKKNVDFSDPYFDATQAVLVDKKSGLTSLADVKAKGKKLG
                     AQAQTTGEDYAKSKGFDPVSFESSDAVLNGLRTGQVQAVVIDYPVVQGWLKDKANADAF
                     KVVDNLNTGEQYGFTVKKGSTKLLAAINKALAGAKSDGTYQKLYEKWIGPYPASASASP
                     SAS"
     misc_feature    complement(2947..2961)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.46"
                     /tool="antismash"
     misc_feature    complement(2991..3007)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.87"
                     /tool="antismash"
     misc_feature    3043..3058
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3179..3712
                     /gene="ectA"
                     /locus_tag="N8I87_RS09465"
                     /old_locus_tag="N8I87_09465"
     CDS             3179..3712
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671932.1"
                     /locus_tag="N8I87_RS09465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09465"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_263207300.1"
                     /transl_table=11
                     /translation="MTAAQADLLIDRPEVTDGGSLWRIAKDSGTLDLNSSYSYLLWCRD
                     FAGTSAVARGADGKPVGFVTGYVRPERPHTLLVWQVAVDAAHRGRGIAAALLDGLTARI
                     GAERPIISVETTITPGNTASERLFTSYADRHGAGLTREVLFDAGLFPDGAHEPEILYRI
                     GPLTPHIAPRSDQP"
     gene            3709..4980
                     /gene="ectB"
                     /locus_tag="N8I87_RS09470"
                     /old_locus_tag="N8I87_09470"
     CDS             3709..4980
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 3.6e-73.
                     Score: 238.4. Matches aSDomain:
                     nrpspksdomains_N8I87_RS09470_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="N8I87_RS09470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09470"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_263207302.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-77, bitscore:
                     260.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSLMYDEDGHRY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLMRDGVTHGLDMSTTAKRSFLQTFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALADLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFRPELDIWEPGEHNGTFRGNNPAFVTATAALETYWTDGSAMEKQTRARGEQIEQAL
                     VSVTEENLADVKEYRGRGLVWGMEFHDKARADRVARRAFELGLLIETSGPEGEVVKLLP
                     ALTVTPEELDEGLSVLARSVRETI"
     aSDomain        3793..4806
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N8I87_RS09470_Aminotran_3.1"
                     /evalue="3.60E-73"
                     /label="N8I87_RS09470_Aminotran_3.1"
                     /locus_tag="N8I87_RS09470"
                     /protein_end="366"
                     /protein_start="28"
                     /score="238.4"
                     /tool="antismash"
                     /translation="FDRAQGSLMYDEDGHRYLDFFAGAGSLNYGHNNPVLKRALIDYLM
                     RDGVTHGLDMSTTAKRSFLQTFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALADLCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFRPELDIWEPGEHNGTFRGNNPAFV
                     TATAALETYWTDGSAMEKQTRARGEQIEQALVSVTEENLADVKEYRGRGLVWGMEFH"
     gene            5001..5405
                     /locus_tag="N8I87_RS09475"
                     /old_locus_tag="N8I87_09475"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="N8I87_RS09475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09475"
                     /product="ectoine synthase"
                     /protein_id="WP_263207304.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.8e-53, bitscore:
                     178.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRSASGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHVEAVVCVEGAAELTDHETGRTYSITPGTMYLLDGHERHTLRVQEDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5411..6295
                     /gene="thpD"
                     /locus_tag="N8I87_RS09480"
                     /old_locus_tag="N8I87_09480"
     CDS             5411..6295
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498585.1"
                     /locus_tag="N8I87_RS09480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09480"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_263207305.1"
                     /sec_met_domain="PhyH (E-value: 2.1e-38, bitscore: 131.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVTDLYPSRGAAEVSVPRQDPVVWGAPDTPGPVPTADLQAYERD
                     GFLAIDQLITDDEVAVYRRELERLVADPAIRADERSVVEPKSKEIRSVFEVHRISEVFA
                     SLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRTV
                     SVSIALTENLDTNGGLMIMPGSHRTFLGCAGATPEDNYKKSLQMQDAGTPSDEALTAMA
                     GEYGIRLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVDNAAVEPFAAPVRR
                     PEFIGARDFTPVR"
     gene            complement(6351..7412)
                     /locus_tag="N8I87_RS09485"
                     /old_locus_tag="N8I87_09485"
     CDS             complement(6351..7412)
                     /NRPS_PKS="Domain: Aminotran_5 (20-347). E-value: 8.7e-25.
                     Score: 79.3. Matches aSDomain:
                     nrpspksdomains_N8I87_RS09485_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671936.1"
                     /locus_tag="N8I87_RS09485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09485"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_263207307.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2e-18, bitscore:
                     65.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METTETFESLVRAEFTPKSTYLNTASNSLLPARTVAALHEAALLR
                     AEGRPLTPLFEDVEACRAAYARLAGVPVTRVAAGASVAAHSGVIAASLPAGAEVLTVED
                     DFTSVLNPFHVRGDLKVRTVPLERVAESVRPDTALVAVSAAQSADGRIADLPALREAAR
                     EHGARTYVDFSQSAGWLPMDADAYDFSAAVSFKWLLGPHGAAFLVVPEDFGGLSPVLAG
                     WVAGEHPWDSCYGPVAELAHSARRFDLSHALFTFAGLRRSLDLIEELGVAAVHAHDVAL
                     ADRFRAGLATLGHAPVPAPGSAIVSVPGLGERQPGLSRAGIELSNRAGNLRASFHLYNT
                     PADVDRLLDVLSD"
     aSDomain        complement(6372..7352)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N8I87_RS09485_Aminotran_5.1"
                     /evalue="8.70E-25"
                     /label="N8I87_RS09485_Aminotran_5.1"
                     /locus_tag="N8I87_RS09485"
                     /protein_end="347"
                     /protein_start="20"
                     /score="79.3"
                     /tool="antismash"
                     /translation="YLNTASNSLLPARTVAALHEAALLRAEGRPLTPLFEDVEACRAAY
                     ARLAGVPVTRVAAGASVAAHSGVIAASLPAGAEVLTVEDDFTSVLNPFHVRGDLKVRTV
                     PLERVAESVRPDTALVAVSAAQSADGRIADLPALREAAREHGARTYVDFSQSAGWLPMD
                     ADAYDFSAAVSFKWLLGPHGAAFLVVPEDFGGLSPVLAGWVAGEHPWDSCYGPVAELAH
                     SARRFDLSHALFTFAGLRRSLDLIEELGVAAVHAHDVALADRFRAGLATLGHAPVPAPG
                     SAIVSVPGLGERQPGLSRAGIELSNRAGNLRASFHLYNTPADVDRL"
     gene            complement(7509..8225)
                     /locus_tag="N8I87_RS09490"
                     /old_locus_tag="N8I87_09490"
     CDS             complement(7509..8225)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140791.1"
                     /locus_tag="N8I87_RS09490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09490"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_263207309.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDEVEVVHRSFELDP
                     GRAKGDVQPVLRMLAKKYGMSEAQAQAGEDNLGAQAAAEGLDYRTRDRDHGNTFDMHRL
                     LHFAKEQGKQDVLIQILYRANFAEERSVFSEGDERLVALAAEAGLDADAARRVLADPAA
                     YADEVRADEREAAELGANGVPFFVLDRKYGVSGAQPAEVFTQALSQAWGARSPLKLVDE
                     GGAEACGPDGCAVPQH"
     misc_feature    8275..8286
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(8301..8807)
                     /locus_tag="N8I87_RS09495"
                     /old_locus_tag="N8I87_09495"
     CDS             complement(8301..8807)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002240.1"
                     /locus_tag="N8I87_RS09495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09495"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_263207311.1"
                     /transl_table=11
                     /translation="MIREHRTVESLVIRTAVRAEAQVIADLHFRARSTYYPDGVPQSDT
                     DWTAAWRSSIERPDGHVLCAVAHGAIVGIASFRTPEDIPAETVKLFQFHIDPDHWRSGL
                     GTALHRACVEEWRADGKRTAVLDVHVDNARAQAFYARQGWAPDPDNPPAEGDHHLFLRF
                     SVSGE"
     gene            complement(8851..9459)
                     /locus_tag="N8I87_RS09500"
                     /old_locus_tag="N8I87_09500"
     CDS             complement(8851..9459)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="N8I87_RS09500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N8I87_09500"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_263207312.1"
                     /transl_table=11
                     /translation="MDLELPELPFLLRTYGPDGHWSYEDGILSGWAGPRQDRFVPPTDE
                     GLDPASDAPRLLGAPEGEFQLIARVTVGFGAAFDAGVLYAHVGDRAWAKLCLEYSPDVP
                     TVCTVVTQGHSDDANAFTVDGSSVWLRISRTGRVFAFHASRDGVRWTFVRLFTLGGEKE
                     TDAALVGFMTQSPLGEGCVVTYDHIEFRPGWPDDLRDGS"
ORIGIN
        1 acgacccaca gctcgtcacg gacctcgtcg gggccgacga ggatccgccc cttcacgtgc
       61 agcaccgcgt gatcgctcat gtaccgcacc ttaggacagc tacccctcgg acttccccac
      121 ctggtccgag gtctcctcct ccacctcggc catcgccggg tcgagcaggc gggagaggaa
      181 gtgccgggtg cgctcgtgct gcgggctgcc gatgacctgg gcgggggggc cgtcctcgac
      241 gatcacgccg ccgtccatga agacgacccg gtcggcgacc tcacgggcga aggtcatctc
      301 atgggtgacg accatcatcg tcatgccctc gtcggcgagc atgcgcatga cggcgagaac
      361 gtcccccacc agctcggggt cgagcgccga ggtcggctcg tcgaacagca tcacctcggg
      421 tcccatcgcg agcgagcggg cgatggcgac gcgctgctgc tggccgccgg agagggagga
      481 cgggtaggcc tccgccttct cggtgaggcc gacccgcgcc aggttctcgg cggcgatcct
      541 cgcggccgtc gccttgtcgc gccggagcac ccggcgctgc ggcagggtga ggttctcggt
      601 caccgacaga tgcgggaaca ggttgaactg ctggaacacc atgccgatac ggcggcgtac
      661 ggcgtcgatg tcgacgtcgg ggtcggtgac ctcggtgccg ccgacgaaga cctggccctt
      721 ggtgggttcc tccagcaggt tgacgcatcg caggagcgtc gacttgccgg agccggacgg
      781 gccgatgacg cagaccacct caccctgggc gatctccagg tcgatgccct tgagcacctg
      841 gttgtcgccg aaggacttgt gcaggtcacg gacctggatc tcagcggtgc cgcggatact
      901 cacttgaccg cctcctgggc attggcctcc aagcggcgcg cgacgaagcc gagcgggatc
      961 gtgacgagca gatagcacag gcccgcgacc aggatcggcg tggagttggc ggtcgtgctg
     1021 gccaggtcac ggccgaactt cgacagttcg cgctcctcca gggtcacccc gagcagcagg
     1081 actagggagg agtccttgaa gagcatgatg aactcgttgg tgagcggcgg gaggacgatc
     1141 cggaacgcct gcgggatgat gatcgagacc atcgcgcggg cgggcgagaa gcccagcgag
     1201 cgggccgcct ccatctgccc cttgggcacc gcctggatgc cggcgcggaa cgtctccgcc
     1261 atgtaggcgg ctccgatcag accgagcccg attgcggcct tgccgtaggt gccgcccggg
     1321 atctccgtgc cggggaaggc cagcggcacg gccacgccga tgaagacgaa gatcagcagt
     1381 gcgggcaggc cgcggaagac ctcgatgaag acgctcgcga cccagcggta cgggcccacg
     1441 gacgacagcc gcatcagcgc gacgaccagc ccgagggcca tggcgagcac gaagccggac
     1501 accgtgtaga gcacggtgtt cttcagcgcc agcgtgatga cgtcggggaa catctgcttg
     1561 gcgatgtccg gctgcgcgaa ctggttccgc agccgccccc agtccgccgt gaccgcgagg
     1621 gcgatcacgg cggcggcgaa gacggtgtac tggacgccac gcgacaggct gcgcttctgg
     1681 cgccgggtaa gacccttttt cttcggctgg agtgctgcgt ctgtgtcggt catggggtca
     1741 ggacgcggag ggcgaggcgg acgcggacgc ggggtacggg ccgatccact tctcgtacag
     1801 cttctggtac gtgccgtcgg acttggcgcc ggcgagcgcc ttgttgatcg cggcgaggag
     1861 cttggtgctg cccttcttca ccgtgaagcc gtactgctca ccggtgttga ggttgtcgac
     1921 caccttgaag gcgtccgcgt tggccttgtc cttcagccag ccctggacga ccgggtagtc
     1981 gatgacgacc gcctggacct ggccggtgcg caggccgttg aggaccgcgt cggaggactc
     2041 gaaggagacc gggtcgaagc ccttgctctt cgcgtagtcc tcgccggtgg tctgcgcctg
     2101 ggcgccgagc ttcttgccct tggccttgac gtcggcgagc gaggtgaggc cgctcttctt
     2161 gtcgacgagg acggcctggg tggcgtcgaa gtacgggtcg gagaagtcga cgttcttctt
     2221 gcgctcggcc gtgatggtca tgcccgcggc ggccacgtcg cactggccgg agttcaggaa
     2281 cgcgccggtc ttgaagttct cgaacggcgt gtcgaggatc gtctgcttca ccccgaggtc
     2341 cttggcgacg aggtcgatca gtgacacgtc gaagccctgc accttgccgt cgatctccga
     2401 ctggaagggc gggtagggca ggtgggtgca ggtggtgagc tgcccggcct tggcgagctc
     2461 gaccccgccg acggccgtct tcttggcgcc gccgccgttg tcgctcgacg agcagccggc
     2521 cacgagcacg agcccgaccg tcgcggtggt tgccgccagg acgcgggccc ggcgcccgaa
     2581 gagcgtgttc acgaggggtg acctcctgtg ggggagcttt ggattccggt tgcaggagct
     2641 ccgattataa ggagatgttt gggtctctca aaccaaaccc atggcagcgg ggtcacacgg
     2701 cctgcgagtc gacggccgaa ggggcgtgat ctcggccgat cacccggaga tgccggcgct
     2761 ggagcggtgc ctcgagtgga tcgaggcaga gggggtggac gcggtgagcg tccggcgccg
     2821 ggccgccgcg ccggccacgc gggtgcgggc ggtgggtctg ggcggaggtc tggcgccgta
     2881 cgctgcgggg cacccggacg tcctgtggga gcgaatcgac ggagtcaccg gggccggaat
     2941 tcagggaaat ttcggaatca cttttcgtcg atagctttcc cgagttcttc tgcccgcttt
     3001 ttgcggagct aaacaagggt gatttcatcc gggtttcaac cctgtgacac actccacata
     3061 gccagtgtat ttgtcccgtt ttatgcggtg caaatcgcca catttcttca gtcagacccc
     3121 tcccggccac acccccactc cgtgcatttt tgaatttgcc tcgctaaatt caagccgcat
     3181 gactgccgca caagcagacc tgctcatcga ccgaccggag gtgacggacg ggggttccct
     3241 ctggcgtatc gccaaggact ccgggaccct cgacctgaac tcctcgtaca gctatctgct
     3301 gtggtgccgc gacttcgccg gaacatcggc ggtcgcgcgc ggggcggacg ggaagcccgt
     3361 cggattcgtc accggctacg tacggccgga gcgcccccac accctgctcg tctggcaggt
     3421 ggcggtcgac gcggcccacc gggggcgcgg catcgccgcc gcgctgctcg acggactgac
     3481 cgcgcggatc ggcgccgagc gcccgatcat ctccgtcgag acgaccatca cacccggcaa
     3541 caccgcctcc gagcgcctgt tcacctcgta cgccgaccgg cacggcgccg gtctcacccg
     3601 cgaggtgctg ttcgacgcgg gcctgttccc tgacggcgcc cacgagcccg agatcctgta
     3661 ccgcatcggg ccgttgaccc cccacatcgc accgaggagc gatcaaccgt gaccatcacc
     3721 cagcccgacc tcagcgtctt cgagaccctg gagtccgagg tacgcagcta ctgccgcggc
     3781 tggcccaccg tcttcgaccg tgcgcagggc agcctgatgt acgacgagga cggacaccgg
     3841 tacctcgact tcttcgccgg agcgggctcg ctcaactacg gccacaacaa ccccgtcctg
     3901 aaacgggcgt tgatcgacta cctgatgcgc gacggggtca cccacgggct cgacatgtcc
     3961 accacggcca agcggtcctt cctgcagacc ttccaggacc tggtgctgcg gccgcgcgac
     4021 ctgccgtaca aggtcatgtt cccggggccg accggcacca acgccgtgga gtccgcgctg
     4081 aagctggccc ggaaggtgaa gggccgcgag gcgatcgtgt cgttcaccaa cgccttccac
     4141 ggcatgtccc tcggctcgct cgccgtgacc ggcaacgcct tcaagcgggc cggtgccggc
     4201 ataccgctgg tgcacgggac gcccatgccg ttcgacaact acttcgacgg ccaggtcccc
     4261 gacttcctgt ggttcgagcg gctgctggag gaccaggggt ccgggctcaa caagccggcc
     4321 gccgtgatcg tggagaccgt gcagggcgag ggcggcatca acgtcgcgcg tccggagtgg
     4381 ctgcgcgccc tggccgacct gtgcgagcgg caggacatgc tgctgatcgt cgacgacatc
     4441 cagatgggat gcggccgcac cggtgccttc ttctccttcg aggaggccgg gatcgtgccc
     4501 gacatcgtca ccgtgtcgaa gtccatcagc ggttacgggc tgcccatgtc gctctgcctg
     4561 ttccggcccg agctggacat ctgggagccg ggcgagcaca acggcacctt ccgcggcaac
     4621 aaccccgcgt tcgtcaccgc caccgcggcc ctggagacgt actggaccga cgggtccgcc
     4681 atggagaagc agacccgggc ccgcggcgag cagatcgagc aggcgctcgt ctccgtcacc
     4741 gaggagaacc tcgccgatgt gaaggagtac cggggccggg ggctcgtgtg gggcatggag
     4801 ttccacgaca aggcacgcgc cgaccgggtc gcgcggcgtg ccttcgagct cgggctgctc
     4861 atcgagacgt ccggcccgga gggcgaggtc gtcaagctgc tgccggccct caccgtcacc
     4921 cccgaggaac tggacgaggg actgagcgtc ctcgcccggt ccgttcgcga aaccatctag
     4981 caaggaggct gtgcaccacc gtgatcgtcc gttcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcacgtgag gtcggcgtcc ggcacctggg agagcaagcg catcgtcctc gccaaggaac
     5101 gggtcggctt ctccctccac gagaccgttc tgtacgcggg gacggagacg tcgatgtggt
     5161 acgcgaacca cgtcgaggcc gtcgtgtgtg tcgagggcgc ggccgagctc accgaccacg
     5221 agaccgggag gacgtactcc atcactcccg ggaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct ccgtgtccag gaggacttcc gctgcatctg cgtgttcaac ccgcccgtga
     5341 cgggacggga ggaccacgac gagaacggcg tttaccccct gctcaccgaa ccggaggagg
     5401 tgtgagttcc atgaccgtca ccgatctcta tcccagccgc ggcgccgcag aggtgtccgt
     5461 cccgcgtcag gacccggtcg tctggggcgc ccccgacacc cccggcccgg ttccgacggc
     5521 cgacctgcag gcgtacgagc gcgacggctt cctcgcgatc gaccagctga tcaccgatga
     5581 cgaggtcgcc gtctaccggc gtgagctgga gcggctcgtc gccgacccgg cgatccgcgc
     5641 cgacgaacgc tccgtcgtcg aaccgaagtc gaaggagatc cgctccgtct tcgaggtgca
     5701 ccggatcagt gaggtgttcg cctcgctcgt gagggacgag cgggtcgtcg ggcgggcccg
     5761 gcagatcctg ggctcggacg tgtacgtcca ccagtcccgg atcaacgtca agcccgggtt
     5821 cggggccagc gggttctact ggcactcgga cttcgagacc tggcacgccg aggacggtct
     5881 gccgaacatg cggacggtgt ccgtgtcgat cgcgctgacc gagaacctcg acaccaacgg
     5941 cgggctcatg atcatgccgg gctcgcaccg gacgttcctc gggtgcgcgg gagccacgcc
     6001 cgaggacaac tacaagaagt cgctgcagat gcaggacgcg ggcacgccgt ccgacgaggc
     6061 gctgaccgcc atggcgggcg agtacggcat ccggctcttc accggccggg ccggctccgc
     6121 cacctggttc gactgcaact gcatgcacgg gtccggcgac aacataacgc cgttcccgcg
     6181 cagcaacgtc ttcatcgtgt tcaacagcgt ggacaacgcg gcggtcgagc ccttcgcggc
     6241 tccggtacgg cggccggagt tcatcggggc gcgggacttc accccggtgc gctgagctgc
     6301 cgcctgcgct gagctactgc caggccgccg aggccgcccg cgccagaggg tcagtccgac
     6361 aggacgtcca gcaggcggtc gacgtcggcg ggcgtgttgt acaggtggaa cgaggcgcgc
     6421 aggttgcccg cgcggttgga gagttcgatg ccggcgcggc tcagtccggg ctggcgctcc
     6481 ccgagtccgg ggaccgagac gatcgccgag ccgggggcgg gcacgggcgc gtggccgagg
     6541 gtcgcgagtc cggcccggaa ccggtcggcg agggccacgt cgtgggcgtg gacggcggct
     6601 acgccgagtt cctcgatcag gtcgagcgag cggcgcaggc ccgcgaaggt gaacagcgcg
     6661 tggctgaggt cgaagcgccg ggcggagtgg gcgagttcgg cgaccgggcc gtagcagctg
     6721 tcccacgggt gctcgcccgc gacccagccc gcgagcaccg ggctcagccc gccgaagtcc
     6781 tcggggacca cgaggaaggc cgcgccgtgc gggccgagca gccacttgaa ggagaccgcg
     6841 gcggagaagt cgtaggcgtc cgcgtccatc gggagccagc ctgcggactg cgagaagtcg
     6901 acgtaggtgc gtgccccgtg ctcgcgggcg gcctcgcgca gggcgggcag gtcggcgatg
     6961 cgtccgtcgg ccgactgcgc ggcgctgacc gcgaccagcg cggtgtccgg gcggacggac
     7021 tcggcgacgc gctccagcgg caccgtgcgg accttgaggt cgccgcggac gtggaacggg
     7081 ttcaggacgg aggtgaagtc gtcctcgacg gtgaggactt cggcgccggc gggcagcgag
     7141 gcggcgatca ccccactgtg cgcggcgacc gaggcgcccg ccgcgacccg ggtcaccggg
     7201 actccggcga gccgggcgta ggcggcccgg caggcctcca cgtcctcgaa cagcggggtc
     7261 agcggacggc cctcggcccg cagcagcgcc gcctcgtgca gggcggcgac ggtacgggcc
     7321 gggagcaggc tgttgctcgc ggtgttgaga tacgtgctct tcggggtgaa ctcggcgcgg
     7381 acgaggctct cgaacgtctc cgtggtctcc atggatccac tctgcggccg ctgaattcat
     7441 aagtccattg cgaatctata cgtggatcca cttaggaact cttatacgtc cgcgccgacc
     7501 tgcggttttc agtgctgagg caccgcacac ccgtccgggc cgcaggcctc ggcgccgccc
     7561 tcgtcgacga gcttcagcgg agaacgcgca ccccacgcct ggctgagcgc ctgcgtgaac
     7621 acctcggcgg gctgggcccc cgagactccg tacttccggt cgaggacgaa gaacggcacc
     7681 ccgttcgcgc cgagctcggc ggcctcgcgc tcgtcggcgc ggacctcgtc ggcgtacgcg
     7741 gccgggtcgg cgagcaccct gcgggcggcg tcggcgtcca ggcccgcctc ggcggcgagt
     7801 gccacgagcc gctcgtcgcc ctcgctgaag acggagcgct cctcggcgaa gttcgcccgg
     7861 tacaggatct ggatgagcac gtcctgcttg ccctgctcct tggcgaagtg cagcaggcgg
     7921 tgcatgtcga aggtgttgcc gtggtcgcgg tcccgggtgc ggtagtccag gccctcggcg
     7981 gcggcctgcg cgcccaggtt gtcctcgcct gcctgggcct gcgcctcgct catgccgtac
     8041 ttcttggcga gcatcctcag caccggctgg acgtcaccct tggcgcggcc cgggtccagt
     8101 tcgaaggacc ggtgcaccac ctcgacctcg tcgcggtgcg ggaaggccgc cagcgccttc
     8161 tcgaagcggg ccttgcccac gtagcaccag gggcaggcga tgtcgctcca gatctcgacg
     8221 cgcatgtctt cggctctctc caggtcgtac gggcgcggag gctctctccg gccagtacat
     8281 gaacgttcaa gcagagaggt tcattccccg gacacggaga aacgcaggaa gaggtggtga
     8341 tccccttcgg cgggcgggtt gtccgggtcc ggggcccagc cctggcgggc gtagaaggcc
     8401 tgggcgcggg cgttgtccac gtgcacgtcg agcacggcgg tccgcttgcc gtcggcgcgc
     8461 cactcctcca cgcatgcccg gtgcagggcc gtgccgaggc cggagcgcca gtggtcgggg
     8521 tcgatgtgga actggaacag cttgaccgtc tccgcgggga tgtcctcggg ggtgcggaag
     8581 gaggcgatac cgacgatggc gccgtgcgcg acggcacaca gcacatggcc gtccggccgc
     8641 tcgatggaac tccgccaggc ggcggtccag tccgtgtcgg actgcgggac gccgtccggg
     8701 tagtaggtcg agcgtgcccg gaagtgcagg tcggcgatga cctgggcctc ggcgcgcacg
     8761 gctgtcctga tcaccaggga ttcgacggtc cgatgctctc tgatcatgca gcggaagacg
     8821 taccgggagg cggtccggtt ccgccgcggc tcagctcccg tcgcgcaggt cgtccggcca
     8881 gccgggcctg aactcgatgt ggtcgtacgt caccacgcag ccctccccca gcggcgactg
     8941 ggtcatgaag ccgaccagcg ccgcgtccgt ctccttctcc ccgcccagcg tgaagagccg
     9001 cacgaaggtc cagcgcacgc cgtcgcggga ggcgtggaag gcgaagacac ggcccgtgcg
     9061 gctgatccgc agccagaccg aactgccgtc cacggtgaag gcgttggcgt cgtcggagtg
     9121 tccctgggtg acgaccgtgc agacggtggg gacatccggg gagtactcca ggcagagctt
     9181 ggcccaggcc cggtcgccca cgtgggcgta gagcacaccc gcgtcgaacg cggcgccgaa
     9241 cccgacggtg acccgggcga tcagctggaa ctccccctcc ggcgccccga gcagccgggg
     9301 cgcgtcggag gcggggtcca gcccctcgtc ggtgggcggc acgaaccggt cctgccgggg
     9361 cccggcccag ccgctgagga tgccgtcctc gtaggaccag tgaccgtcgg ggccgtaagt
     9421 acgcagaagg aagggaagtt cgggaagttc caggtccatg agcagagtct ctcagctccc
     9481 gatctccgtc accgaccccc cgtacgggtt cggcgttccg gatgaccgcc gaagtgccgg
     9541 ggccgacgga cttcgaggtc gaggtcgtcg ccgggtacgg tccctcggtg ctgggccggt
     9601 gccactgcga cacccgtcgg ccagccgctc ttcgtcggcc cctccgccgc gaggcgcagg
     9661 tcagcgttcc agacggccgt tgaaacggcg cggcaggccc agggggttgt cgtcgcggag
     9721 ttccaacggg agcagggcct caggggcgtt ctggtagacg accgggcgca gccagcgctc
     9781 gatggccgta ccgccgacgg acgtcgacgt ggacgtcgtc gccgggtacg ggccgccgtg
     9841 gtgctgggcc ggtgccaccg cgacacccgt cggccagccg ttcaccagaa cccggccggc
     9901 cagcggggtc agctccgcca agagctccgc gccgcggccc tcgcccgcgg cctccttagt
     9961 cgagaggtgc acggtcgcgg tgaggttgcc ggggagccgg gacagtacgg cggtcgcctc
    10021 gtcctggtcg tcgtagcgga ccaccacggt caccggtccg aagcactcct ccaggagcag
    10081 gtcgtacgct ccctcctcgg ccagtcggct cgccggaacc gtcaggaagc cggggctgac
    10141 cgtgtgttcg ctgcccgcgc cgggggtcac cggggtctcc acgccggaca gcgcggcccg
    10201 ctcggcgacg ccggcgacga agttgtcccg catgcggtgg tcgagcagga cccccgcctc
    10261 ggtgtcgctg accgcgtccg tgagcgactt gagcagaccg tcgcccccgg cgccggacgg
    10321 caccagcacc aggcccggct tcacacagaa ctgccccacg cccagcgtca tcgacccggc
    10381 gagaccggcg ccgatggcct cggcg
//
