LOCUS       NZ_CP104900            10840 bp    DNA     circular CON 13-OCT-2024
DEFINITION  Shewanella seohaensis strain KCTC 23556 chromosome, complete genome.
ACCESSION   NZ_CP104900
VERSION     NZ_CP104900.1
KEYWORDS    RefSeq.
SOURCE      Shewanella seohaensis
  ORGANISM  Shewanella seohaensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
            Shewanellaceae; Shewanella.
REFERENCE   1  (bases 1 to 4751888)
  AUTHORS   Liu,G.-H.
  TITLE     Taxonomy and metabolic features of ten novel Shewanella species
            tbased on genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4751888)
  AUTHORS   Liu,G.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) Center for Microbial Electrochemistry,
            Fujian Agriculture and Forestry University, shangxiadian road,
            fuzhou 350002, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025449215.1-RS_2024_10_11
            Annotation Date                   :: 10/11/2024 19:44:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,230
            CDSs (total)                      :: 4,090
            Genes (coding)                    :: 3,472
            CDSs (with protein)               :: 3,472
            Genes (RNA)                       :: 140
            rRNAs                             :: 10, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 618
            CDSs (without protein)            :: 618
            Pseudo Genes (ambiguous residues) :: 0 of 618
            Pseudo Genes (frameshifted)       :: 605 of 618
            Pseudo Genes (incomplete)         :: 21 of 618
            Pseudo Genes (internal stop)      :: 5 of 618
            Pseudo Genes (multiple problems)  :: 12 of 618
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:10:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2467982
            Orig. end                         :: 2478822
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104900.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Fujian Academy of
            Agricultural Sciences.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10840
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10840
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10840
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5840](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5840
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            891..2611
                     /gene="ushA"
                     /locus_tag="N7V09_RS11060"
                     /old_locus_tag="N7V09_11060"
                     /pseudo=""
     CDS             891..2611
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ushA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006086184.1"
                     /locus_tag="N7V09_RS11060"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11060"
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTNKLIKGLIATAVLAALAGCNSDDDNTKVPTTCAEAGSACKTFT
                     VLHTNDNHGRFWENSDGEYGMAARKALVDQIRAEVSKNGGQTLLLSGGDINTGVPESDL
                     QDAIPDFTGMNKIGYDAMAVGNHEFDNPLSVLDMQRRLAEFPMLAANIYRKNAEGGQGE
                     RYFEPYKVFDINGLKVAVIGLTTEDTAKIGNPEFISDLIFTDPKVEVAKVIKEIKDAKA
                     ADVIFATTHMGHYADGQNGSNAPGDVAMARALNKGDLQVVIGGHSQNPVCMEPGTENKS
                     YADFKPGDDCAPDQQNGTWIMQAHEWGKYVGRADFEYFNGELHLASYKLIPRTCVNWTK
                     LARKPLSWLALRLSQMLS"
     gene            complement(2705..3064)
                     /locus_tag="N7V09_RS11065"
                     /old_locus_tag="N7V09_11065"
     CDS             complement(2705..3064)
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006082091.1"
                     /locus_tag="N7V09_RS11065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11065"
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="WP_248966957.1"
                     /transl_table=11
                     /translation="MSTTHIRQELSELEANLRKEIGAHPQFIEILGAHYANLSLGELID
                     RLAQSHLAEHPLFCRLNRLDAAFCQLDLGLYGLCSDCETEIEAERLTQDPTEQRCSSCA
                     EHYQHEHRHELRLSH"
     misc_feature    complement(3117..3131)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.79"
                     /tool="antismash"
     misc_feature    3171..3184
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.19"
                     /tool="antismash"
     gene            complement(3228..4175)
                     /locus_tag="N7V09_RS11070"
                     /old_locus_tag="N7V09_11070"
     CDS             complement(3228..4175)
                     /GO_function="GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_process="GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623022.1"
                     /locus_tag="N7V09_RS11070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11070"
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="WP_248966958.1"
                     /transl_table=11
                     /translation="MRVILAPMEGVVDDLMRDILSSINPYDLLVTEFVRVVDQLLPEKV
                     FLKLCPELLNGGFTPSGTPVRVQLLGQEPNCMAENAMRAIELGSHGVDANFGCPAKMVN
                     RSNGGAVLLQYPNTIHDIVRAMRQAVPAEHPVTAKIRLGYEDKSLFMENALAVYEAGAT
                     ELAIHARSKVDGYKPPAYWEYITEVRERLPIPVIANGEIWNREDAERCMAVTGCDSIMI
                     GRGAISLPNLADTIKTGAAPYSWADTLGLMLSYTQRELSGRKSDYYPARIKQWFSYLNR
                     QYPEADTLFRELRIYKTTEEIVRVLEEAQQRLGE"
     misc_feature    4336..4345
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            4435..4866
                     /locus_tag="N7V09_RS11075"
                     /old_locus_tag="N7V09_11075"
     CDS             4435..4866
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012089495.1"
                     /locus_tag="N7V09_RS11075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11075"
                     /product="hypothetical protein"
                     /protein_id="WP_086904622.1"
                     /transl_table=11
                     /translation="MKSVKQTAVAVALGTVVVGSAFAVNAQTNPFGFETMEAGYQIVGS
                     EGKCGEGKCGGDMKKAAAEKAKEGKCGEAKCGADMKKAAADKAHEGKCGEAKCGADMKK
                     AAADKAHEGKCGEAKCGGDMKKTAEKVEAKADAVKKEMK"
     gene            5001..5840
                     /locus_tag="N7V09_RS11080"
                     /old_locus_tag="N7V09_11080"
     CDS             5001..5840
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011072092.1"
                     /locus_tag="N7V09_RS11080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11080"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_248966959.1"
                     /sec_met_domain="DUF692 (E-value: 2.8e-108, bitscore:
                     359.1, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQESGLVGLGLRREMLSEFCQALPTAIDFLEVAPENWMTLGGKYG
                     RQFRQLTEQHTFFCHGLSLSIGSPAPLDLEFVRQVKAFMDLHQIEVYSEHLSYCSGGGH
                     LYDLMPIPFTDDAVKHVATRVKQVEDILERPLILENISFYAAPSAQMSEQAFLTAVLEE
                     ADCKLLLDVNNIYVNSVNHQYDAEAYLKAMPTERIAYLHIAGHYQQSETLLIDTHGSDI
                     NDPVWALLDKCYAQHGVLPTLLERDFNLPETVQLLDEINQIHAYQARAKNRLDKRIA"
     gene            5840..6580
                     /locus_tag="N7V09_RS11085"
                     /old_locus_tag="N7V09_11085"
     CDS             5840..6580
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011626437.1"
                     /locus_tag="N7V09_RS11085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11085"
                     /product="DUF2063 domain-containing protein"
                     /protein_id="WP_248966960.1"
                     /transl_table=11
                     /translation="MDFTQVQQSFIDYIRDPQNPVPSGVPLERMQVYRELFFNNVMGFV
                     SNAFPVLKSLYSDAQWQVLVQAFFSQHDCKSPIFIDIAGEFLQFLQFEYSPAESDPPFM
                     LELAHYEWLELRVATAQQVDQQERLSSADILQRNLCLAATASVAQYQYPVQQIRQDYRP
                     TEALPQPVFFCLYQDEASEVGFLQLTPLSAQVLGYLVEQGQSRLDDILNWLQGIYPQMQ
                     PEILTQGCIQLLEQLAVKGIVRGI"
     misc_feature    6675..6690
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     gene            6737..6859
                     /locus_tag="N7V09_RS11090"
                     /old_locus_tag="N7V09_11090"
     CDS             6737..6859
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623018.1"
                     /locus_tag="N7V09_RS11090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11090"
                     /product="hypothetical protein"
                     /protein_id="WP_011623018.1"
                     /transl_table=11
                     /translation="MSSQPFKDPFNFMYFIGFILVLLLPTLPASLTWLKQLGLI"
     misc_feature    complement(6870..6884)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 25.29"
                     /tool="antismash"
     gene            6960..7328
                     /locus_tag="N7V09_RS11095"
                     /old_locus_tag="N7V09_11095"
     CDS             6960..7328
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011626436.1"
                     /locus_tag="N7V09_RS11095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11095"
                     /product="YbaN family protein"
                     /protein_id="WP_011626436.1"
                     /transl_table=11
                     /translation="MILKRGLFLLIGCLALGLGLLGIVLPLLPTVPFILLAAFCFARSS
                     ERLHQWLMTHPWFADALTQWQQHRAMRPGLKRRAMLLTGLSFSVSILVVPIVWVKLLLL
                     TMACGLLWYLKTIPEIES"
     gene            7510..8061
                     /gene="apt"
                     /locus_tag="N7V09_RS11100"
                     /old_locus_tag="N7V09_11100"
     CDS             7510..8061
                     /EC_number="2.4.2.7"
                     /GO_function="GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /gene="apt"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020911865.1"
                     /locus_tag="N7V09_RS11100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_11100"
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="WP_011623016.1"
                     /transl_table=11
                     /translation="MAMNTETLSLIKQSIKTIPNYPKEGILFRDVTSLLENAAAYKATI
                     DLLVEQYRNKGFTKIVGTEARGFLFGAPLALELGIGFVPVRKPGKLPRATISQSYELEY
                     GHDSLEIHTDAITANDKVLVVDDLLATGGTIEATVKLIRQLGGEVQDAAFVISLPDLGG
                     EARLTALGLELVKLCEFEGE"
     misc_feature    complement(8114..8130)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.96"
                     /tool="antismash"
     gene            8191..8514
                     /locus_tag="N7V09_RS21530"
     CDS             8191..8514
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024575.5"
                     /locus_tag="N7V09_RS21530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_390903710.1"
                     /transl_table=11
                     /translation="MSYQVLARKWRPATFEQMVGQSHVLHALTNALTQQRLHHAYLFTG
                     TRGVGKTSLARLFAKGLNCEKGVTASPCGVCGSCVEIAQGRFVDLIEVDAASRTKVDDT
                     QNS"
     misc_feature    8980..8996
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.82"
                     /tool="antismash"
     misc_feature    9176..9191
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.05"
                     /tool="antismash"
     misc_feature    complement(9948..9963)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.99"
                     /tool="antismash"
     misc_feature    complement(10035..10049)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.82"
                     /tool="antismash"
ORIGIN
        1 ctttatcttc ccacacgcgc cagagcaagc ggtgaccatc aacggcggct acattatgcg
       61 tgcttggtat gacattaaaa gcatggattt gcacgataga gccgatatgc agggcgtgat
      121 ggcctcggag ctgagtgtac aggccttgat tgacgaacaa atcgccgcgg ggatccccag
      181 tgagcgcatc gtcttagccg gatttagcca aggcggggtg atgagcctat ttaccggatt
      241 acgttatccc caaaaacttg ctggcattat ggcgctgtcc tgttatttac cgacgggtga
      301 tgtgttaccc agccaattaa gtgcagccaa tgcgaaaacg cctattttac atcagcacgg
      361 tgaacaggat gatgtagtgc cactatccgc aggtttatta gcgaaagacg cgttaatagc
      421 tgggggatat tcggtgcaat ggcaaaccta cccgatgccg cattcggtta tcccggtaca
      481 attaaaagca ataagcactt ggttacaaca gtgttttgaa atgtagaaaa atctaaatgc
      541 cgataaaaac accatgcggt aaattaaatt ttggttgcat ggtgttttat atagagaaca
      601 atccattata ttgccagtgc tatctaagtt gcttaaatta aagcgttaaa tcttttcttt
      661 ttaaatgcct tccccattaa attgtgaaat aaaaaataag tcgtttagct tattttttat
      721 ttgaaacaaa tctgtcttaa aactcattaa agatcccaca ttaaagtcac gtttattaat
      781 aaaaatgaca caattcgtct ttattctttg ttattatgcg tggcgcgaga gtcgatggta
      841 ctcgcacaat ctatctatga taaaataatt aaaatcaagg ggttgatgta atgacaaata
      901 agcttattaa aggattaatt gcgactgcag tgcttgctgc gctagcaggt tgtaatagtg
      961 atgatgataa tactaaagta ccaacaacct gtgccgaagc gggcagcgct tgtaaaacat
     1021 ttactgtatt acacaccaac gataaccatg gccgtttctg ggaaaacagt gatggcgaat
     1081 atggtatggc ggctcgtaaa gccctagtcg atcaaattcg tgctgaagtc agcaagaatg
     1141 gcggccaaac attattattg tctggcggtg atattaacac cggcgtacca gaatctgatt
     1201 tacaagatgc gattcctgac tttactggta tgaacaaaat tggttacgac gcaatggcag
     1261 ttggtaacca tgaattcgat aacccattat ctgttttaga tatgcagcgt cgtttagccg
     1321 aattcccaat gctggcggca aacatttacc gtaaaaacgc tgagggtggt caaggtgaac
     1381 gttatttcga gccttacaaa gtgtttgata ttaacggtct taaagtggct gtgattggtt
     1441 taaccaccga agacactgca aaaatcggta acccagaatt tatcagcgat ttgattttca
     1501 ccgatcctaa agtggaagtg gcgaaagtta tcaaagaaat taaagacgcc aaagcggctg
     1561 atgtgatctt cgcaacgact cacatgggtc actatgccga tggtcaaaac ggtagcaacg
     1621 ctcctggcga cgtcgcaatg gcgcgtgcgt tgaataaagg cgacctacaa gttgttatcg
     1681 gtggtcactc acaaaaccca gtgtgtatgg agccgggtac tgaaaacaag tcttacgccg
     1741 acttcaaacc tggtgatgat tgtgcgccag accaacaaaa cggcacttgg atcatgcaag
     1801 cccacgaatg gggtaagtat gttggccgtg cggatttcga atacttcaat ggcgaattgc
     1861 acttagctag ctacaagctg atccccagaa catgcgtaaa ctggacgaag ctggcaagaa
     1921 aaccactgag ttggctggca ctaagattga gccagatgct gagctaaaag agctgctgtc
     1981 ttactaccaa gagaaaggcc aagctaagtt agacgaagtg atcgcgacta ccgatgcgct
     2041 gctcgatggc gaacgtgcaa acgtacgtaa caagcaaact aacttaggcc gtatgttagc
     2101 catggcgcaa agcggtaaag tgtctgctga ctttggtgta atgaactcag gtggtgtgcg
     2161 cgcttctatc caagcgggcg acattactta ccgtgatgtg ctgacggttc aacctttcgg
     2221 caacatggtg accctgaacg aaatgacagg tactgaagtc gcggcttact taggcgctgt
     2281 gggcagtatc caaatcggtt cgggtggtta tgcgcagatc actggcgtga aaatgaccat
     2341 cgactgtgtg gctaagacgg ctaatatcag cgaaatcaac ggcaaagagt tcagcgcaac
     2401 ggcaaactat aagttcaccg tacctagctt taacgctgca ggtggtgacg gttatccaaa
     2461 actggctagc ccaatccaaa cgggttatgt ggatgcggac ttactctatt cattcctgaa
     2521 agagaaacaa gctatcgttg ctgcagacta caacccagtg ggcgacattg tttacgaaaa
     2581 ctcaaacagt gttgaaggct gtaagctgta atcacacgcg ataaattatc gtaaaaaatg
     2641 ccactcattt gagtggcatt tttattggtg aactgactca gtgaacagat aaagtctaaa
     2701 gctgttaatg gcttagtcta agctcatggc gatgttcatg ctgataatgc tcggcgcagc
     2761 tgctgcaacg ttgctcggtg ggatcttgag ttaaacgctc ggcttcgatt tcagtttcgc
     2821 aatctgagca gaggccgtat aagcctaagt cgagttgaca gaaggcggca tcgaggcggt
     2881 ttaaacgaca aaatagcgga tgttctgcga ggtgactctg tgccaggcgg tcaattaact
     2941 cgcctaagct gagattggca taatgcgccc caagtatttc aataaactga gggtgcgcgc
     3001 cgatttcctt ccttagatta gcttcaagct ctgacaattc ttgtctgata tgggtggtgc
     3061 tcacgtaaaa tcgcctgttt tatctgactc tggacattca gtctaatagt tccactatca
     3121 acgactatga taaagatcaa gtatctgtcc gatttgcagg gatttagtgg tgttttaaat
     3181 aaaatttcac cactttctca tgggattatc ctatgcgcta tcagtggcta ttcgcctagg
     3241 cgctgctgcg cctcttccag cactcgcaca atttcctctg tggttttata gatgcgtagc
     3301 tccctaaaca gggtgtctgc ctcggggtat tggcgattca aataactaaa ccactgctta
     3361 attcgcgccg gataataatc acttttacgg ccgctcagtt cgcgttgggt gtagctcagc
     3421 atcaaaccta atgtatcggc ccagctgtaa ggtgccgcgc cagtcttaat cgtgtcggca
     3481 agattgggca gcgaaatcgc gccgcggcca atcataatgc tgtcgcagcc cgtcaccgcc
     3541 atacaacgct cggcatcttc gcggttccaa atctctccat tagcaatcac tggaatgggc
     3601 aaacgttcgc gcacttcggt gatgtattcc cagtaggcgg gcggtttgta accatcgacc
     3661 ttactgcggg catggatagc cagctctgtc gcgcccgctt cataaacggc cagcgcattt
     3721 tccataaaca gggatttgtc ttcgtagcct aaacgaattt tcgccgtcac tggatgttcg
     3781 gcgggcacgg cttggcgcat ggcgcggaca atatcgtgaa tcgtattagg gtattgcagc
     3841 aacaccgcgc cgccattact gcggttaacc atcttagcgg ggcaaccaaa gtttgcatcg
     3901 acgccgtggg agccaagctc aatcgcgcgc atcgcgtttt cggccatgca gttgggctct
     3961 tgccctaaaa gctgtacacg aaccggcgtg cccgagggag taaagccgcc attaagcagt
     4021 tcggggcaga gtttgagaaa cactttttcg ggtaagagtt ggtcaaccac gcgcacaaat
     4081 tcggtgacga gcaaatcgta ggggttaatt gaagacaaaa tatctcgcat taagtcgtca
     4141 accacgcctt ccatcggagc caaaattacg cgcactgcaa caaacccata tccagtttca
     4201 aagggcgtgc attctatcct attggactgt ggttacaaag cattgcgagg gaagggtaag
     4261 agcagttttt ggggattttt acgactaaat tctgtttcct atcagcatat tttgattatt
     4321 tctgtgattt ggtgatattt tttgaaatga aaccgagatg gggcgggtct tttgcagtaa
     4381 gccagctaca gcactgttgt tggccaccat tcatcaaata aaaggaagaa atatatgaag
     4441 tcagttaagc aaacagccgt tgcagttgcg ttaggtacag tggtggtcgg ttctgcgttc
     4501 gccgtgaatg cgcaaaccaa cccattcgga tttgaaacca tggaagcggg ttatcaaatc
     4561 gtcggttcag aaggtaaatg cggcgaaggt aaatgtggtg gcgatatgaa aaaagccgca
     4621 gctgaaaaag ccaaagaagg taaatgtggc gaagcaaaat gtggtgccga catgaagaaa
     4681 gcggccgcag ataaagccca cgaaggtaaa tgcggtgaag ctaaatgtgg cgccgatatg
     4741 aaaaaagctg cagccgataa agcccacgaa ggtaaatgtg gtgaagctaa atgcggtggc
     4801 gatatgaaga aaaccgctga aaaagtcgaa gctaaggctg acgcagtcaa aaaagaaatg
     4861 aaataagcgc gagtcgatac tatcgagctt cagctctaca cttggccagc ctcagctggc
     4921 ctttgtttag ctgcattcga gtgtgggctt gccagaacga aaaggtaatc tcgacacgcc
     4981 ctaggtaaga ggtgaaagca atgcaagaga gtggattggt tggattaggc ttacggcgtg
     5041 aaatgctcag tgagttttgc caagcgctgc caacggctat cgatttttta gaggtcgccc
     5101 ctgaaaactg gatgacgctc ggtggaaaat acggcaggca atttaggcag cttactgagc
     5161 aacatacttt tttctgccat ggtctgtcac tctctattgg cagtcctgcc cccttagatt
     5221 tggaatttgt ccgccaagtg aaggcgttta tggatctgca ccaaattgaa gtctattcgg
     5281 agcatttaag ttattgctcg ggtggcggtc atttatacga tcttatgccc attcctttta
     5341 ccgatgacgc cgttaagcat gtggcgacgc gggtaaaaca ggtggaggat attttagagc
     5401 ggcctttgat cctcgagaat atttcctttt atgctgcgcc gagcgcacag atgagtgagc
     5461 aggcgttttt gaccgcagtg ttagaagagg ctgactgtaa gctgctgtta gatgtgaata
     5521 atatctatgt gaactcggta aatcatcaat acgatgccga ggcttattta aaggccatgc
     5581 caacggagcg catcgcctat ttgcatatcg cagggcatta ccaacagtct gaaaccttgc
     5641 tgatcgacac ccatggcagc gacatcaacg atccggtttg ggcactgttg gataagtgct
     5701 atgcccagca tggcgtcttg ccgacactgc tcgagcgtga ctttaatttg cctgaaacgg
     5761 tgcagttatt ggatgagatt aatcaaatcc atgcctatca agcgcgggcg aaaaatcgtt
     5821 tggataagag gattgcctga tggattttac gcaagtacag cagtctttta tcgactatat
     5881 tcgcgatcca caaaacccag tgccgagcgg tgtgccctta gagcggatgc aggtttatcg
     5941 agagctgttt tttaataatg tgatgggttt tgtgtccaat gcttttcccg ttcttaaaag
     6001 tctttatagc gatgcacagt ggcaagtctt agtgcaggct tttttcagtc aacatgattg
     6061 taagtcccca atttttattg atattgccgg agagttttta caattcctgc aatttgaata
     6121 ttcaccagca gaaagtgatc cgccttttat gttggagctg gcgcactatg aatggcttga
     6181 gcttcgagtg gcaacggcgc aacaagttga ccagcaagag agattgtcca gcgcagatat
     6241 tttgcagcgc aatttgtgtt tagcggcaac ggcgagtgtg gcacagtacc agtatccggt
     6301 gcagcagatc cgccaagatt atcgccccac agaggcgctg ccgcagccag tgtttttttg
     6361 cctctatcag gatgaagcga gcgaggtggg cttcttacaa ttaacgccgt taagtgcgca
     6421 agtgctggga tatttggtag aacaaggaca gagtcgcctc gatgacatac taaactggct
     6481 gcaagggatt tatccgcaaa tgcaaccgga gatcttaacc caaggctgta ttcagttact
     6541 cgaacaactc gccgttaaag ggatagtgcg cggaatttaa gctgcatgtt gctaaaatca
     6601 gcaaaataaa tgcaataacc ttacataaaa agggtttttg ttgatttgac aaaccgctac
     6661 aatggcgccc gttttgtgat cttaatcact gtacgattcc tgcgcactta tctttgcaca
     6721 catttgagga gctgtaatga gcagtcagcc atttaaagac ccatttaact ttatgtattt
     6781 tattggtttt attttagtct tactgttgcc gacgctacca gcaagcctga cttggttaaa
     6841 gcaattaggc ttaatttaag caggttttaa tctcaaattg agtatactac tggccactct
     6901 tagagtggct tttgtttttc aggtttgagg cacctacata ctatgatcat ttacaagcta
     6961 tgatattgaa acgcggtttg tttttattga taggttgctt ggcgttgggc cttggactgc
     7021 tgggtatcgt attgcctcta ttgccgacag taccgtttat tctattggcg gctttttgtt
     7081 ttgcccgctc aagcgagcgc ttacaccagt ggttgatgac acacccgtgg tttgccgatg
     7141 cgctgaccca atggcaacaa cacagggcga tgcgccccgg ccttaaacgc cgagcgatgt
     7201 tgctcacggg actcagtttt agcgttagca ttcttgtggt cccgattgta tgggtgaagt
     7261 tactgctgct caccatggct tgcggattac tttggtatct taaaaccatt cctgaaatcg
     7321 aatcctaagc acatttgatc taaggcacaa attggtaacc aattcgtcac ttgaatacgc
     7381 tgcttattgc aactcaaacc aaagcggcat aagctttgtg cgaattatta aagctcgtcc
     7441 acactcgctg tggacgtttt gtttttaccg ccagcagtta cgcgtggcga aacgattaag
     7501 ttaaattaaa tggctatgaa tacagaaacc ttatccttga taaagcaaag cattaaaacg
     7561 attcctaatt atcctaagga agggattcta tttcgcgatg tgaccagtct gctggaaaat
     7621 gccgcggcct ataaagcgac tatcgattta ttagttgagc aatatcgcaa taaaggcttt
     7681 accaagattg tgggcactga agcccgtggt ttcttatttg gtgcgccttt agcactggaa
     7741 ttaggcattg gttttgtgcc tgttcgtaag cctggtaagt tgccacgcgc gaccattagc
     7801 caaagctatg agctggaata tggccacgat agccttgaaa tccataccga tgcgattacc
     7861 gcgaatgaca aagtattagt ggttgacgat ttgctggcca ctggcggcac tatcgaagcc
     7921 acagtcaaac ttatccgcca actgggcggt gaagtgcaag atgcggcttt tgtgatttca
     7981 ttacccgatc tcggtggcga agcccgctta acggccttag gtcttgagtt ggttaaactt
     8041 tgtgagtttg aaggcgaata attcttcagc caaaggcgcc tagggatagt ttaatggcat
     8101 gtcgttaatt gacatgttat cttgcaacta tctcggggcg cgatcccctt ttttgtacca
     8161 acattcttgt catgcacatt ggggagttcc atgtcatatc aggtgttagc cagaaaatgg
     8221 cgccctgcca cattcgaaca gatggtcggc caaagccacg tgttgcacgc gctcacgaat
     8281 gcgttaacgc aacaacgttt acaccatgct tatcttttta cgggtactcg cggtgtgggc
     8341 aagaccagtt tggcgcgcct cttcgctaaa ggcctcaact gtgaaaaggg cgtaacggct
     8401 tccccctgtg gtgtgtgtgg cagctgtgtc gaaattgccc aaggccgctt tgttgacttg
     8461 atcgaagtcg atgccgcctc gcgcactaag gtcgatgaca cccagaactc ttagataatg
     8521 tgcaatatcg tccgacccgt ggccgtttta aggtctacct gattgacgaa gtacacatgc
     8581 tctcccgcag cagttttaat gcactgctaa aaaccctcga agaaccgcca gagcatgtga
     8641 aatttttgct ggcgacgacc gatccgcaaa agctgccggt gactgtgtta tcccgctgtc
     8701 tgcaatttaa tttaaagagc ttaacccagc aggaaattgg cactcagcta cagcatattt
     8761 taacccaaga gcaattaccc tttgagcacg aagccttaac tctgctcgcc aaagcggcca
     8821 atggcagtat gcgcgatgcc ttaagtttga ccgatcaagc tattgccttc ggtggtggca
     8881 cagtaatgct caatcaagtg caatctatgc tgggtagcat cgatgagcag catgtgctgc
     8941 gcttactcaa agccttaacc gatgccgata ttggcgtttt gatgcaaagc tgcgcacagg
     9001 tgctcgccta tggcgcggac gcccaagaag tgctgcgcag tttactggaa ttattgcatc
     9061 aaattaccct gacgcaattt gctcctgccg cggcgcagca atccctttac agcgcgcaaa
     9121 ttcaagcctt tgccgagcag ttagcgccgg aacaagtgca gttgtattac cagattttac
     9181 tgactggtcg taaagactta ccccatgcgc cggatcctaa atcgggtctc gaaatggcgt
     9241 tgctccgagc ggtggcattt gttcccgaaa agcccgtgaa gcgttggcag gtcgaagatg
     9301 ctgctaaggt gagcttgcca gctcagcagt caatgtctgc gacgcccaca ccaaatatgc
     9361 ccgcggcgga accagctgta aatcaagcag tggctgaaaa aactccgcta accgctgagc
     9421 gtgactcaca ggctctgcca caagctgcaa tctctgagtc tatggctcga tctcaggaat
     9481 cttcagcttc tcaagccgaa gctgcgcagc aagctgtatc cgaggatgct ttagttggcg
     9541 aggatgcact tgatgaagag tctacactca acgccacttt gattgctgag cagcaagtga
     9601 tcttaagcca agcccaaagc caaggctttg gcgcaagtac cgaggtgata tcgcgtactg
     9661 aggccgtctt aatttcagaa tcaagccctg aatcgaactc agtatctacg cctgcatcga
     9721 atctttatgc tgagcataat caagcggcga tggtggatcc cgaaagctct ggccagaaac
     9781 cttctcctgc ggtgttaaca gaacccgttg ccttatctca tgatgatatc gaggacgata
     9841 ctcaagcgag tgttaatcag agtgcagatg cgctagcgtc agatgaatat tctgcagagg
     9901 atttcggaca atacgattat gccgctgcgc cgctcgatgc ctatcaggat gattattcgc
     9961 aatttatggc tgaggagcag ggcgggttct ctgggatgga agatagctcg ttgatgagcg
    10021 acagtgctca gtctatcgac catagtgatg ttgtgcatga gtctgccaat gaccccaaat
    10081 cgcagtcttt tgccgacaat tcagtttcaa caactcctcc agtttcaaca gctactgtat
    10141 ccctcgagga tgacgatatt ttatcggcgg tattagcggc acgcgactcc ttactctccg
    10201 atctcgatgc gctcagcacc aaggaaggtg atggaaaaaa gccagcagcg gaagttaagc
    10261 ttaaaacccc aggccagagt gcgcataccg cgccagtttc taacgcggca ttaaataaca
    10321 cgtcctcagt tgatgcatct attgagccgc aagtcgctaa aaacgcgtct gataacgagt
    10381 ttgagctgcc ctttgatgat gatttcgacg ccgagctcca tcttgagtca cagtcaaatg
    10441 ttacgccaac tccgccgctt gatccgaacg atcgcccgcc ttgggaagag gcgcccgtcg
    10501 cggcgatgga tgaagccaat gcgcccgctg caagtgttaa cctacagact gaaacccctg
    10561 tcgcgccgca aacagctgtt gaggatttag gcgctgcgcc cgttgccgct caaacatcgc
    10621 caacggacaa cgaggcgaca cgggttgtgc aagcgttaga gcaagtggca gtacaagaaa
    10681 gcgagccagc tgcgcaaagc cttgagccaa atacgcagcc tcctcaggcg caggaactta
    10741 tacagccaca aactcaacca caaactcagc agctaattca gcaaccgatt cagcagttaa
    10801 ctcagcaatt ggcatcggtg cagtcaccat caagcgagac
//
