LOCUS       NZ_CP104900            20882 bp    DNA     circular CON 13-OCT-2024
DEFINITION  Shewanella seohaensis strain KCTC 23556 chromosome, complete genome.
ACCESSION   NZ_CP104900
VERSION     NZ_CP104900.1
KEYWORDS    RefSeq.
SOURCE      Shewanella seohaensis
  ORGANISM  Shewanella seohaensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
            Shewanellaceae; Shewanella.
REFERENCE   1  (bases 1 to 4751888)
  AUTHORS   Liu,G.-H.
  TITLE     Taxonomy and metabolic features of ten novel Shewanella species
            tbased on genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4751888)
  AUTHORS   Liu,G.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) Center for Microbial Electrochemistry,
            Fujian Agriculture and Forestry University, shangxiadian road,
            fuzhou 350002, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025449215.1-RS_2024_10_11
            Annotation Date                   :: 10/11/2024 19:44:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,230
            CDSs (total)                      :: 4,090
            Genes (coding)                    :: 3,472
            CDSs (with protein)               :: 3,472
            Genes (RNA)                       :: 140
            rRNAs                             :: 10, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 618
            CDSs (without protein)            :: 618
            Pseudo Genes (ambiguous residues) :: 0 of 618
            Pseudo Genes (frameshifted)       :: 605 of 618
            Pseudo Genes (incomplete)         :: 21 of 618
            Pseudo Genes (internal stop)      :: 5 of 618
            Pseudo Genes (multiple problems)  :: 12 of 618
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:10:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1938767
            Orig. end                         :: 1959649
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104900.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Fujian Academy of
            Agricultural Sciences.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20882
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="3"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20882
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20882
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10882](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10882)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            complement(90..833)
                     /locus_tag="N7V09_RS08680"
                     /old_locus_tag="N7V09_08680"
     CDS             complement(90..833)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012278159.1"
                     /locus_tag="N7V09_RS08680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08680"
                     /product="(Fe-S)-binding protein"
                     /protein_id="WP_011623487.1"
                     /transl_table=11
                     /translation="MKIALFIPCLVNQMMPDVAIATLELLEKLGHQVILPAGQTCCGQP
                     MTNSGCFDAARSTTLKLLNAFKGVECDAIVCPAASCLVAAKENFHEFDSSPEAQAVIDK
                     LYELTEFLHDVAPIPAFNKPFAHKISLQLSCHGIRMLSLATPSEQMGPRFNKVEAVLAN
                     IAGIDIVYPDRRDECCGFGGTFAVDEGAVSAKMGKDKAQAHAATGAQYVVGFDPSCLLH
                     LDGMIRRQQLPIEIRHIAQVLNAAL"
     misc_feature    1075..1090
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.81"
                     /tool="antismash"
     gene            complement(1136..3939)
                     /locus_tag="N7V09_RS08685"
                     /old_locus_tag="N7V09_08685"
                     /pseudo=""
     CDS             complement(1136..3939)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1261: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846281.1"
                     /locus_tag="N7V09_RS08685"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08685"
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSINYDAVYKELIQQLGESAVSNDPVRRFAWSTDASYFRIVPEIV
                     VHADNLEQAKQTLAIARAHKVPVTFRAAGTSLSGQAIGEGILLILGHDGFRTIEISSDS
                     NKITLGAAVIGADANAALKPLNKKIGPDPATLASAMVGGIVSNNASGMCCGTAQNSYQT
                     IASAKLLFADGTELNTGCDKSKAAFTQSHGELLDSLASLAKLTRSNDVLAQRIRKNTPS
                     KTLRVIASTP"
     gene            complement(4002..5648)
                     /locus_tag="N7V09_RS08690"
                     /old_locus_tag="N7V09_08690"
     CDS             complement(4002..5648)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012588195.1"
                     /locus_tag="N7V09_RS08690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08690"
                     /product="L-lactate permease"
                     /protein_id="WP_086902307.1"
                     /transl_table=11
                     /translation="MTILQLLASLTPVISVMIFLVLLRMPASKAMPISMIVTALAAVFI
                     WQMDTTLLAASVLEGLLSAITPLTIIFGAVFLLNTLKYSGAMDTIRAGFTNISADARVQ
                     VIIICWLFGAFIEGSAGFGTPAAIGAPLLVLLGVPPVAAAVVALIADSACVSFGAIGLP
                     VLFGMEQGLTQGGVSMAAAQIAEHGGTYPDFAQFIAMHMITIDLITGTLIPLVMVSILT
                     GFFGRNKSFKEGLAIWKFAIFAGLAFTVPAWIINYIAGPEFPSVIGSLIGMAMVIPVAR
                     KGYLLPKTPWNDFAENDNQEESAKIETTTKFSQIAAWTPYIIMAALLVLSRTVAPLKAW
                     LSGFNISWTGLLGTELKASFATLYAPGAFFVAVCILGFFLFKMKSPAIKQSIGVSCKSM
                     LPTIISLGASVPMVKIFLNSGANGAGLASMPVALADMLAHSMGAVWAWMAPIVGIFGAF
                     LSGSATFSNMMFSGLQYSVADNIGMNHALVLALQGIGANAGNMMCVMNVVAAATVVGMA
                     GRESEIIRKTMPVAIGYALLAGTIATLWGGF"
     misc_feature    5892..5907
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     misc_feature    6051..6065
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 20.87"
                     /tool="antismash"
     gene            complement(6397..7275)
                     /gene="nadK"
                     /locus_tag="N7V09_RS08695"
                     /old_locus_tag="N7V09_08695"
     CDS             complement(6397..7275)
                     /EC_number="2.7.1.23"
                     /GO_function="GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="nadK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005497624.1"
                     /locus_tag="N7V09_RS08695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08695"
                     /product="NAD(+) kinase"
                     /protein_id="WP_084789193.1"
                     /transl_table=11
                     /translation="MTTKFHTIGLIGKPHHPGTNQTLKRLHHWLTVQGYEVLVEERVAS
                     ELGTNIVAVDLLEIGARCDLAIVVGGDGNMLGAARVLARFDVGVIGVNRGNLGFLTDLP
                     PDAFEEALAKVLDGEFDTEHRFLLEAEVYRHGMLKASNTAVNEAVLHPGKIAHMIEFEV
                     YIDNQFMYSQRADGMIVSTPTGSTAYALSAGGAILTPNLQALILVPMFPHTLSCRPIVV
                     DACSTIKMVVSPENGENLEVSCDGHVHLAVLPGDEIIVRRSSEQLRLIHPKGHNYFHVL
                     RSKLGWGSKLF"
     gene            7375..7986
                     /gene="grpE"
                     /locus_tag="N7V09_RS08700"
                     /old_locus_tag="N7V09_08700"
     CDS             7375..7986
                     /GO_function="GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /GO_function="GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_function="GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /gene="grpE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011790104.1"
                     /locus_tag="N7V09_RS08700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08700"
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="WP_086902308.1"
                     /transl_table=11
                     /translation="MSNESIKAEQDLIQEGVESEVSTEEASLIDELTQANFRIEELEQL
                     LADALAKVEEQKDSVIRAAAEVDNIRRRAAMDVEKANKFALEKFANELLPVLDNMERAL
                     QGTNPQDETTKALFEGVELTQKSFLTAVAKFGVKPIDPQGQAFNPDQHQAIGMQPSAEY
                     PANTVMLVMQKGYELNSRLLRPAMVMVSQGGPSQEINIEA"
     gene            complement(8111..9979)
                     /gene="dxs"
                     /locus_tag="N7V09_RS08705"
                     /old_locus_tag="N7V09_08705"
     CDS             complement(8111..9979)
                     /EC_number="2.2.1.7"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="dxs"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623482.1"
                     /locus_tag="N7V09_RS08705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08705"
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="WP_248966785.1"
                     /sec_met_domain="DXP_synthase_N (E-value: 4.6e-121,
                     bitscore: 401.1, seeds: 174, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.6e-07, bitscore:
                     27.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLDISQFPVLAQANTPNELRQLPQALLPQLADELREFLLKSVGM
                     SSGHFASGLGTVELTVALHYVYNTPFDRLIWDVGHQAYPHKILTGRRDRMHTIRQKNGL
                     HPFPWREESEYDTFSVGHSGTSVSAALAMAVAAEKEQAGRKVVAVIGDGAMTGGMVFEA
                     MNHAGDLHNDMLMVLNDNEMSISENVGALNNHLAQLMSGRLYTTIRESSKKVLKGMPVI
                     KEMAKRTEEHLKGMVVPGTLFEELGFNYIGPIDGHDVDALVETLRNMRSLKGPQVLHIM
                     TKKGRGYEPAEKDPIGWHAVPKFDPSQFKKPATKPGLPTFSQVFGKWLCDIAEQDEKVL
                     GITPAMREGSGMVEFSQRFPKQYFDAAIAEQHAVTLGAGFACEGFKPVVAIYSTFLQRG
                     YDQLIHDVALQRLPVLFAIDRGGIVGADGPTHQGAFDLSFMRCIPNMVIMAPSDENECR
                     QMLYTGYCYDAGPSAVRYPRGSATGATQVEAMTALPIGKGVIKRLGKRIAMLNFGTTLA
                     AALTAAESLDATVVDMRFVKPLDVDLVKEMAQTHDVLVTVEENAIMGGAGSGVLELLQK
                     LKMPKPVLQIGLPDEFIKHGSPEEVTHDLQLDAEGMLAQINAFLAD"
     gene            complement(10001..10882)
                     /gene="ispA"
                     /locus_tag="N7V09_RS08710"
                     /old_locus_tag="N7V09_08710"
     CDS             complement(10001..10882)
                     /EC_number="2.5.1.10"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ispA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006080883.1"
                     /locus_tag="N7V09_RS08710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08710"
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="WP_248966786.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.9e-64, bitscore:
                     215.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVNAIKEYQLRVDDQLKQRFSTLEDTAPQLKAAMTHGALLGGKR
                     IRPFLVYSVGQMFGVSLEKLDACAAAIECIHAYSLIHDDLPAMDDDDLRRGQPTVHIAF
                     DEATAILAGDALQTLAFEIVTEAVPGISSEQHIAMVRALAKASGYRGMCGGQAIDLAST
                     DKLINLKALTQLHNMKTGALISCAVELALIAANAPKDEYQTMMEFAHAVGLAFQVQDDI
                     LDIIATTEELGKPQGSDAESNKSTFPQLLGLQGAKDTAAQLIQEALSALAKLPYNSQLI
                     ADFARYIIERRI"
     gene            complement(10885..11124)
                     /gene="xseB"
                     /locus_tag="N7V09_RS08715"
                     /old_locus_tag="N7V09_08715"
     CDS             complement(10885..11124)
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /gene="xseB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623480.1"
                     /locus_tag="N7V09_RS08715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08715"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_011623480.1"
                     /transl_table=11
                     /translation="MAKKPENLSFEESLGELERIVAELEQGDVSLDDALKQFERGINLV
                     RNSQAKLEQAQQKVAILLKQDENAPLSPYAVEGE"
     misc_feature    11227..11240
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.48"
                     /tool="antismash"
     misc_feature    complement(11228..11241)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.07"
                     /tool="antismash"
     misc_feature    11228..11242
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.67"
                     /tool="antismash"
     gene            11438..12205
                     /gene="pomA"
                     /locus_tag="N7V09_RS08720"
                     /old_locus_tag="N7V09_08720"
     CDS             11438..12205
                     /codon_start=1
                     /gene="pomA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071707.1"
                     /locus_tag="N7V09_RS08720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08720"
                     /product="flagellar motor protein PomA"
                     /protein_id="WP_011717762.1"
                     /transl_table=11
                     /translation="MDLATVIGLVGGFGFILWAMMTSGGLAIFIDVPSVFIVFGGSFFV
                     VMMKFNLKQFLGAVKIAVKAFMFKIDKPEDLIEQSVTMADAARKGGFLALEEAQISNSF
                     MQKAVDMLVDGHDGEVVRAALEKDIALTEERHRNGIAIFRAFGDVGPAMGMIGTLVGLV
                     AMLSNMSDPKSIGPSMAVALLTTLYGAVVANMVCIPIADKLTLRMGEEMLNRNLIMDAV
                     LAIQDGQNPRVIEGFLKNYLAEKQRKIDTTDGE"
     gene            12209..13129
                     /locus_tag="N7V09_RS08725"
                     /old_locus_tag="N7V09_08725"
     CDS             12209..13129
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623478.1"
                     /locus_tag="N7V09_RS08725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08725"
                     /product="flagellar motor protein MotB"
                     /protein_id="WP_011623478.1"
                     /transl_table=11
                     /translation="MAKCNCPPPGAPLWLATFADLMSLLMCFFVLLLSFSEMDVMKFKQ
                     IAGSMKYAFGVQNKVEVKDIPKGTSVIALEFRPGRPDPTPIEIINQQTNEMTEPVLDYQ
                     AGEDDSAGGVQQQNGSQRGGEASATAQETADAVKAEAAAAQDKINQQVKKMAQELNNEI
                     TDGAIEIESLGQQIIIRIREKGSFASGSGFLQPRFKPVVRSVGELLKDVPGIITVSGYT
                     DDMQISDELYSSNWDLSSKRAVAVADVLLQVKGFDPKRMKVVGMASNNPIVPNDSPENR
                     ARNRRVEIAIEQGKAKESEEIQVGK"
     gene            13346..14799
                     /gene="thiI"
                     /locus_tag="N7V09_RS08730"
                     /old_locus_tag="N7V09_08730"
                     /pseudo=""
     CDS             13346..14799
                     /GO_function="GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071709.1"
                     /locus_tag="N7V09_RS08730"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08730"
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKFIVKLYPEIMMKSKPVRMRFTKMLETNIRNVLKKVDEDAKVQR
                     QWDRIWVKVPNDKPELALAFGERLACIPGIAHVVQVDEYSFTSVDDIYQQVLPVYRDQI
                     AGKTFCVRVKRTGSHDFNSIEVERYVGGGLNQFTDAIGVRLKNPEVTVNLEIEGDKLYM
                     VTKRIEGLGGFPMATQEDVLSLISGGFDSGVSSYQFIKKGARTHYCFFNLGGAQHEIGV
                     KQVAYHLWKTYGESHKVKFVSVPFEPVVAEILEKIDNGQMGVVLKRMMMRTAARIAERM
                     GIQAIVTGESLGQVSSQTLTNLNVIDRCTDMLILRPLIAMDKQDIINECRRIGTEDFAK
                     SMPEYCGVISQKPTVKAVLAKVEAEETKFSEDLIDRIVEQAVAIDIREIAEQMNTRITE
                     TETVVAIDTTKW"
     gene            complement(14951..15184)
                     /locus_tag="N7V09_RS08735"
                     /old_locus_tag="N7V09_08735"
     CDS             complement(14951..15184)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623476.1"
                     /locus_tag="N7V09_RS08735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08735"
                     /product="hypothetical protein"
                     /protein_id="WP_248966788.1"
                     /transl_table=11
                     /translation="MFNLKSAFALVLVSALSANVSANVIDAEIIIDTSDLQASINAELT
                     AMMEEMHQEALQDPTLLLADEVLPAQQVVKTE"
     gene            15361..16272
                     /locus_tag="N7V09_RS08740"
                     /old_locus_tag="N7V09_08740"
     CDS             15361..16272
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013050410.1"
                     /locus_tag="N7V09_RS08740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08740"
                     /product="transcriptional regulator GcvA"
                     /protein_id="WP_011626781.1"
                     /transl_table=11
                     /translation="MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALE
                     DFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQLADATDRLLARSAIGSLTVSMSPSF
                     AIQWLVPRLSKFSEKNPDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKLRNE
                     VLIPVCSPMLLNGPKPLSKPEDLKYHTLLHDMSRHDWQAWFRQCGISDINVNQGPIFSH
                     SSLVLQAAAHGQGVALGYSVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKV
                     VAFREWMLDMFAEESRSELLPG"
     gene            16253..16923
                     /locus_tag="N7V09_RS08745"
                     /old_locus_tag="N7V09_08745"
                     /pseudo=""
     CDS             16253..16923
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012588192.1"
                     /locus_tag="N7V09_RS08745"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08745"
                     /product="alpha/beta fold hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSCCPDNPLTNEDVASSAYVLAGEPSETLILFAHGAGANRDSDFM
                     CQMAAGLVAKGFQVMRFNFPYMQANAVDGKKRPQIERQSCLPVLARCSMWRTLNLRLSV
                     WS"
     gene            17011..17402
                     /locus_tag="N7V09_RS08750"
                     /old_locus_tag="N7V09_08750"
                     /pseudo=""
     CDS             17011..17402
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006080891.1"
                     /locus_tag="N7V09_RS08750"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08750"
                     /product="DUF423 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRKGLLLLAAVSGFLRSR"
     gene            17556..18641
                     /gene="rlmM"
                     /locus_tag="N7V09_RS08755"
                     /old_locus_tag="N7V09_08755"
     CDS             17556..18641
                     /EC_number="2.1.1.186"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623472.1"
                     /locus_tag="N7V09_RS08755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08755"
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="WP_011623472.1"
                     /transl_table=11
                     /translation="MKNLFLFCRAGYEKECAAEIQQRAAELNVGGFVKANNNDAYVVYQ
                     CFEEDGGDTLVKQLPLDSLIFARQMFAASELLADLPESDRVSPIVAALSDVSKAGELRV
                     ETPDTNEAKELSAFCRKFTVPLRQHLKKSGSLLAQENPKRPIIHVCFIGPGRAYAGYSF
                     SNNSSPYFMGIPRLKMAADAPSRSSLKLDEAFAQFVPKEEQEERVRSGMNAVDLGACPG
                     GWTYQLVRRGMMVSAVDNGPMNEKLMETGQVKHFREDGFRFEPQRKNIYWLVCDMVEKP
                     ARVAELIEAWAINGWFKEAIFNLKLPMKSRYKEVMAILNTMQEILKENGINEFQLQCKH
                     LYHDRDEVTVHLWIRPSQAWN"
     gene            complement(18775..19785)
                     /locus_tag="N7V09_RS08760"
                     /old_locus_tag="N7V09_08760"
     CDS             complement(18775..19785)
                     /EC_number="1.1.1.41"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623471.1"
                     /locus_tag="N7V09_RS08760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N7V09_08760"
                     /product="isocitrate dehydrogenase"
                     /protein_id="WP_011717756.1"
                     /transl_table=11
                     /translation="MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTAL
                     EKQGELLPQRTLELIEKNRITLKGPLTTPVGEGFTSINVTLRKKFGLYANVRPVLSFKG
                     TQARYENIDIITVRENTEGMYSGHGQKVSEDGTTAEATSIVTRQGAEQIATFAYELARK
                     ENRKKVTIVHKANIMKSTSGLFLKVAREVSQRYPDIKTEEMIVDATCMKLVMNPENFDV
                     IVTTNLFGDILSDLCAGLVGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVI
                     LASIQMLEYLGMADKADLIRKAVSAVIEEGDRTTRDLGGTHGTTDFTQAVLDRLS"
ORIGIN
        1 tttcatcacg gcagaaaata tcggccttgt gggcgtgaac ctgtgaaccc gttgcctcgt
       61 gattatgttg atatgccata gtgggtctcc tagagcgcgg cgttaagtac ttgcgctatg
      121 tggcgaattt caattggcaa ttgttgtctg cggatcatgc catcgaggtg taataggcat
      181 gaagggtcga agccaaccac atattgcgcg cccgttgccg cgtgggcttg ggccttgtct
      241 ttacccatct tggccgatac agcgccttca tcaacggcaa aagtaccacc aaagccacag
      301 cattcatcgc gacggtctgg atagacaatg tcgattccgg cgatattggc gagcacggct
      361 tcaactttgt tgaatcgtgg gcccatttgc tcgcttggcg tggcaaggct caacatacga
      421 atgccgtggc agctcaactg caggctgatc ttatgggcaa aaggcttgtt gaacgctggg
      481 attggggcga catcgtggag gaactcggtc agctcataga gtttgtcgat aacggcttgg
      541 gcttctgggc tgctgtcaaa ctcgtggaag ttttctttgg cggccactaa acaagacgcg
      601 gcagggcaaa caatggcatc acattccaca cccttgaaag cattgagtaa tttcaaggta
      661 gtactgcgtg ctgcatcgaa gcaacccgag ttggtcatag gttggccaca gcatgtttgg
      721 cctgcgggta gaatcacttg gtgtccgagt ttttccagta attccagggt ggcaatcgcc
      781 acgtctggca tcatttggtt aacgagacac gggatgaaaa gagcgatttt cattgtgttt
      841 ttctctttct tgttagtgaa cgacattgcc ttgcactgtg accccaaagt ccacacaaat
      901 gctaaggtag ttgatcaaat ataggcttat cttaagtaaa taagtagcgc taatttagta
      961 aatgatcttg ttgatttttg caaaaatgag aatattttaa tgtgttgatt ttaatctgtt
     1021 aaatatgatt tttgttttgt gatttacctc tcctctcaat agtgtttaac gataaatgag
     1081 aatagttttc ttctgataaa aaaacggggc catttctagc cccgttgagt actttttagc
     1141 ggctgcactc ttcgagcaga tacgctaagt ggcggtagga aatgccactg tgctgcgtta
     1201 agccgacctc acacatacgg ttggcgtaat agccttcctt aatctcgacg ggaatgagct
     1261 ttttgatatt acgcagggcg ctggcgttga tttctggctt atacaggcct ttttcgccgg
     1321 cgtaaccaca gcagtcaatg cccgcaggtt tgagcacttg gctcgaacaa gcatcggcaa
     1381 tggcttgcat cttaggctct aacttcatct tacgagcgct acaacctaag tgcagagcca
     1441 cattgacctt tttgttgatg ctcagcttat ccagcagctt gtcgtgcata aactcaacta
     1501 agtcggtgat ttgcacctga gggttacccg tgagggtacg ataggtacaa gacagggcat
     1561 cgactaatac tgggattttt ccgccattac tcatcttgct gaccacatcg ataagctctt
     1621 ggcgtttggc gtcggcgttt ttaaagtcgc ccttagattc ccacatctgg ccgcagcata
     1681 aatcgcgggt tttctctggg gtaataacgt tatagcccgc acgctcgagc agagttacaa
     1741 ccacctctgg caaggtgcga ttatcgggat ccttaggtgt tggaccaaag gtacggccgc
     1801 cgcaggctgg gaagtaaacc acggtttctt ggcccgcttt ggctggggac ggtttaggca
     1861 gtttgccacc tttagggaag tctggattcc aatagggcac ttctttggag agtaagcggc
     1921 cagttttcat cagcgcgttg gtgataccat cgccggtgat tttatggatg actcccaaca
     1981 cgtcaaagcc agtgctgatc acttggttga cggcgccaaa gtgtttggct tggaaatcca
     2041 acactttttg ctcagtggtg ctgatatagg gcgtgcgcag cttacggact aactgaccca
     2101 tgctgttatc gacggggcag gcaattgtac agagctgaca agccgcgcag gtatcaatca
     2161 catcatactt ggcatcggca cgcattttat cggcggcggc tttatcgcct gattgctcta
     2221 agcgctcgat ttcacgtagg gtggcgatcc gctgacgtgg cgagaagttc agcgccgaag
     2281 tcgggcaggt cttttcgcag aaaccacatt cgatacattt atcgacaaaa tcatcgacta
     2341 cagggcaagg cttgatgttt ttcacgtgca ctgtgctgtc gtcgtttaag ataacgccag
     2401 ggttgagcag gccttctggg tcgaagatat gcttgatgcg tttcatgagg gtgtaagcat
     2461 cggcgcccca ttccatttca acgaaaggtg caacggcgcg gcctgtgccg tgctcggcct
     2521 tcatcgaacc atcgtattta tggatcacca tctcggccac atcctgcata aagccttgga
     2581 agcgctcgat gtcggcttgg gaggcgaagg tcggggtgat aataaagtgg aagttacccg
     2641 ccagcgcatg gccgtaaatc acgccttcgg gatagccgtg cttatggaat agctcggtta
     2701 aatcggcggc ggccgcggct aaatgttcga cgttaaaggc cacgtcttcg atgatgactg
     2761 agctgccctt tggtcgctcg ccaccaataa tcgggaacag gccagagcgc attgcccagt
     2821 atttgctgta cacgttggcg tcgctactaa agctaatcgg acgctcggtt ttgatgtgcg
     2881 cgagtttagc aatcacatct tgggtatagc tttctaaggt ttgggcatcg ttagcgcggg
     2941 actcaatcaa tagaatagcc gcgccttcag gcagttcatt gagccagtct ggcatgcctt
     3001 ttttgcctgt gacggccttg atggatgccc agtcgagtaa ttcggcggcg gcaacgctat
     3061 cgccaataat cggtggaatg gcacttgccg catccaccat gttgaaaaat accgccatgg
     3121 ccgaggcttt aaatttggct tcatcgaccg tgtggtaagt gacttcttcg acaaaggcta
     3181 aggtgccttc ggcgccgacg atcaagtggt taatcaaatc gaacgggtct tcaaaatcca
     3241 ctagggcgtt gatgctataa cccgtagtgt ttttgatgga gtatttttgc gaatacgctg
     3301 cgcgagcacg tcattactgc gggtcaattt agccagtgag gccagtgagt cgagcagctc
     3361 gccgtggctt tgggtgaagg cggctttaga cttatcgcaa cctgtgttga gctcggtacc
     3421 atcggcaaac agcagtttag ccgaggcgat ggtttggtaa ctgttttgcg ccgtaccaca
     3481 gcacatgccc gaggcgttgt tagacacaat accgccgacc atggccgagg caagggtcgc
     3541 cggatcggga ccgattttct tattgagagg cttgagcgcc gcgttggcat cggcaccaat
     3601 caccgccgcg cctaaggtga tcttattgct gtcggaactg atttcaatgg ttctaaaacc
     3661 gtcgtggccc aagatcaata aaatcccttc gccaatggct tggcccgata aactggtacc
     3721 cgccgcacgg aaggttactg gcactttatg ggcacgggcg atggctaagg tctgcttggc
     3781 ctgttcgagg ttatccgcgt ggaccacaat ttcaggcacg atacggaaat aactggcgtc
     3841 ggttgaccac gcaaagcggc gcacagggtc atttgagacg gctgactcgc cgagttgttg
     3901 aataagctct ttataaacag cgtcataatt aatagacata agttactctc tcaacgctaa
     3961 aaatgactaa ggccgccaag cggccttagg tttcgattgg cttagaagcc accccaaaga
     4021 gttgcgatgg tgcctgcaag tagcgcataa ccaatcgcca caggcatagt tttacgaata
     4081 atttctgatt ctctacccgc catacctaca acggtcgcgg cggctactac gttcatcaca
     4141 cacatcatgt taccagcgtt cgcaccgata ccttgtagtg ctaatactag cgcgtggttc
     4201 atgccaatgt tgtcggccac actgtattgc agaccggaga acatcatgtt ggagaaagtg
     4261 gccgagcctg aaaggaaggc gccgaagatc cccacaatcg gtgccatcca tgcccacaca
     4321 gcgcccatcg aatgggccag catgtcggct aaggcaacag gcatagacgc gaggcccgca
     4381 ccgttagcac cagagtttaa gaagattttc accataggca cggatgcgcc taatgagatg
     4441 atagtcggta acatcgactt gcatgacacg ccgatggatt gcttaatcgc agggcttttc
     4501 atcttgaaca ggaagaaacc taagatacac acagccacga agaaggcgcc tggtgcgtac
     4561 agggtcgcaa agctggcttt aagttcagtc cccaataaac cagtccagct gatgttgaaa
     4621 cctgataacc aggctttgag tggtgcaaca gtacgtgata gcactaatag cgctgccatg
     4681 atgatataag gtgtccaagc agcgatttgt gagaacttag tggtggtctc aattttggcg
     4741 ctttcttctt ggttatcatt ttcggcaaaa tcattccaag gcgtttttgg caataagtaa
     4801 cccttacgtg caactgggat aaccatcgcc ataccgatga gagaaccaat cacggatggg
     4861 aactcagggc ccgcaatata gttgatgatc caagcgggaa cggtaaaggc aagacccgcg
     4921 aaaatcgcaa acttccaaat ggctaagcct tctttaaatg acttattacg gccgaagaag
     4981 cccgtcagaa tcgacaccat caccagtgga attaaggtac ctgtaattaa gtcgatagtg
     5041 atcatatgca ttgcaatgaa ctgggcaaag tcaggataag tgccaccgtg ctcggcaatt
     5101 tgcgccgcag ccatgctcac accaccttgg gttaagcctt gttccatacc aaacagcacg
     5161 ggtaaaccaa tcgcaccgaa ggatacgcag gccgagtcgg caatcagcgc cacaacggcg
     5221 gcggcaacgg gcggaacccc cagcaatacc agcagcggcg caccaatcgc ggcaggtgta
     5281 ccaaagcccg cgctcccttc gataaaggca ccgaataacc aacagataat aatcacttgc
     5341 acacgggcat cggcactgat gttagtgaaa cccgcgcgaa tggtatccat ggccccagag
     5401 tatttcaggg tgttaagcag gaataccgcg ccgaagatga tggttaaggg ggtgatggct
     5461 gacagcagtc cttcgagaac cgatgcggct aacagtgtgg tatccatttg ccaaataaac
     5521 acggcggcaa gcgccgtaac tatcatggag atgggcatag ctttggacgc tggcattctg
     5581 agtaatacga gaaagatcat tacactgatc actggcgtca agctggcgag gagttgaagt
     5641 attgtcatgc ttgcctctac ataaagtggt ctgtcttatt atttatattt ggatcttgct
     5701 agtgttttcc tgtttaaccc tcggcggata aatcagcagt ttcaactgcc aatgccaaga
     5761 gtcacaacat tctgtggtgt gtactagacg ctattcgggg cttaactaat atgatctaga
     5821 tcattttggc tgctgtataa aatgctaatt tacagtgctg cgggaggctt ctctcatagg
     5881 attttcctaa ctgtgatcca tgtcactcga aggtggtttt ttatatttcg ataatgggat
     5941 ttttcattaa tttctattcc ttaaggttat aaaaatcgag atatccgcta aattagctat
     6001 cagtattgca attaactgat ccatttattt cattaatgag tacttaatgg atttttaaga
     6061 caatattaag gcgtgactca tgtctcgttt ttatgtagca gtaaaaatca ctccgctaaa
     6121 ttgagtttgg taagctgttg aataaatagg tatttattga gtgttgcacc gcatggaata
     6181 gtgagagcaa gatcaaattt tagtgatgac taaatttcgc gtgatgaata tcactcaatg
     6241 aattttgttt taatctgaaa tttaataacg gttaataaag ctgtttacag ccgcgcagcc
     6301 gcaacacacc attctgttaa caattggtgt ggagatttac ctgagtgact aatgatggcg
     6361 agccgttgcg gccattatgc ctcgcctaat gtgggcttaa aacagcttac tgccccagcc
     6421 gagcttactg cgtaacacat ggaagtaatt atggccttta gggtggataa gcctgagctg
     6481 ctcagagctt cggcgcacta tgatttcatc gcctggtaag acggctaaat gcacgtggcc
     6541 atcacaactt acttcaagat tttcgccatt ttcaggggag acgaccatct taatggtgct
     6601 gcaggcatcg actactatgg ggcgacacga cagtgtatgt ggaaacatag gcactaagat
     6661 taatgcctgt aaattagggg tcagaatggc gcctcctgca gacagggcat aggcggtaga
     6721 tcccgtcggg gttgagacta tcatgccatc ggcgcgttgg ctgtacatga attggttgtc
     6781 tatatagact tcaaattcaa tcatatgggc gattttaccc ggatggagta ccgcttcatt
     6841 aacggcggta ttgctcgctt ttaacatgcc atggcgatag acctcggcct cgagtaaaaa
     6901 gcggtgctcg gtatcaaatt ccccatcgag cactttggcg agggcttctt caaaggcatc
     6961 gggcggtaaa tccgttaaaa agcccaaatt gccacggtta acaccaatca cacctacatc
     7021 gaagcgcgcc agtactctgg cggcaccgag catattgcca tcaccaccca cgactatggc
     7081 taaatcgcag cgtgcgccaa tctctaataa atcgaccgcg acgatatttg tccctaactc
     7141 gctggcaaca cgctcttcca cgaggacttc atacccttgc accgtcagcc agtggtgcag
     7201 acgtttgagg gtttgatttg tccctgggtg gtgtggcttg ccaatcaggc caatggtgtg
     7261 gaactttgtg gtcatattta tgctagatct ggccttaggc cttgaaacgt caaaattgat
     7321 ccccataata tgcccagagt atttagaaac aaagcatttt tagctggagt gaaaatgagc
     7381 aacgagtcga ttaaagcaga acaggatctg atccaagagg gtgtagagtc tgaagtttct
     7441 accgaagaag cgagtctgat tgacgagctt acccaagcca atttccgtat tgaagagctg
     7501 gaacagttac tcgctgacgc tttggcgaaa gttgaagagc aaaaagattc agttatccgt
     7561 gctgctgccg aagtagataa cattcgtcgc cgcgcggcga tggatgtgga aaaggctaac
     7621 aaatttgcat tggaaaaatt cgccaacgaa ttactgccag tgttagacaa tatggaacgt
     7681 gcactgcaag gtactaatcc acaggatgaa actaccaaag cattgttcga aggtgttgag
     7741 ctcacgcaaa agagcttctt aactgccgtg gctaagtttg gggtaaaacc tatcgatcca
     7801 cagggtcaag ctttcaatcc tgaccaacat caagccattg gtatgcagcc aagtgctgag
     7861 tatccagcca atactgtgat gttagtgatg caaaagggtt atgagctaaa cagccgttta
     7921 ctgcgtccag ccatggtgat ggtgtcacag ggcggcccaa gccaagaaat caacattgaa
     7981 gcctaagcta acgcttagcg ttaactaata aaaaggactg ccgatgcagt cctttttatt
     8041 tgtctgttac tttttatttg ccgttaatcg tgattcacca cgattgatgg ggctaagctc
     8101 ccatcaatat tcaatcggca agaaaggcat taatttgcgc taacatacct tcggcatcga
     8161 gttgtaaatc atgggtcact tcttctggag agccatgttt aataaactca tcgggtaggc
     8221 cgatttgcag cacaggtttg ggcattttaa gcttttgcag cagctctaaa acgccagagc
     8281 ctgcgccgcc cataatggcg ttttcttcca ctgtgacaag cacatcgtgg gtttgcgcca
     8341 tctctttaac caaatccaca tcgagtggct tcacaaaacg catatcgacg acggtggcgt
     8401 ctaagctttc tgcggcggtt aaggcggcgg ctaaggtggt gccaaagttc agcatggcga
     8461 tacgtttgcc taaacgctta atcacgcctt tacctattgg caatgctgtc atggcttcta
     8521 cctgagtcgc gccggtggcg ctgccccttg gataacgtac cgcactcggg ccagcatcat
     8581 agcaataacc ggtatagagc atttggcgac attcattttc atccgatggc gccatgatca
     8641 ccatattcgg gatgcagcgc ataaagctta aatcgaatgc cccttggtgg gttgggccgt
     8701 cggcaccgac gataccgcca cggtcaatgg cgaacaatac aggcaagcgc tgcagtgcca
     8761 catcgtggat cagttgatca taaccccgtt gcaggaaggt cgagtaaatg gcgaccacag
     8821 gcttaaagcc ttcacaggca aatccggcac ctaaagtcac cgcatgttgc tcggcaatag
     8881 cggcatcgaa atattgtttc gggaagcgct gtgaaaattc caccatgccc gaaccttcac
     8941 gcatggcagg ggtaatgcct aagacttttt cgtcctgctc ggcaatatca cataaccatt
     9001 tgccaaacac ttgggagaag gtcggcagtc ccggcttagt ggctggcttc ttaaactgtg
     9061 aaggatcaaa tttaggtacc gcgtgccaac cgataggatc tttctcggcc ggttcataac
     9121 cacgaccttt tttggtcatg atgtgcagca cttgtggccc ctttagagag cgcatattgc
     9181 gcagggtttc cactagggcg tcgacatcgt ggccatcaat tgggccgata tagttaaagc
     9241 ctaactcttc aaacagagtg ccaggtacga ccataccctt aaggtgttct tcggtgcgct
     9301 tggccatctc tttgatgacc ggcatgccct tgagcacttt cttgctgctt tctcgaatcg
     9361 tggtgtagag gcggcccgac atcaattgcg ctaagtgatt gttgagcgcg ccgacgtttt
     9421 ctgaaatcga catttcgtta tcgttcagca ccatcagcat atcgttgtgc aaatcacccg
     9481 cgtgattcat ggcctcgaac accataccgc ccgtcatggc accatcgcca ataacggcga
     9541 ccactttacg gcctgcctgt tctttttcgg cggcaacggc catggctaat gcggcactga
     9601 cggatgtgcc ggagtgtccg acgctgaagg tgtcgtattc actttcttcc cgccaaggga
     9661 aagggtgtaa accgttcttc tgcctaatgg tgtgcattct gtcgcggcgg ccggtgagga
     9721 ttttatgggg ataggcttgg tggccaacgt cccaaatcaa acgatcaaac ggggtgttat
     9781 agacataatg cagggcaaca gtcagttcga ctgtgccgag tcctgaggca aaatgtccgc
     9841 tggacatgcc aacggactta agaagaaact cccgcaactc atcggccaat tgaggtaaca
     9901 gggcctgagg aagctggcgg agttcattag gagtgttggc ctgagccaac acgggaaact
     9961 gtgaaatgtc caaactcata gcgagatact tcgtctctta ttatattctt cgctcaatga
    10021 tatagcgggc gaagtctgcg attaactggc tattgtatgg caatttagcc agcgctgata
    10081 gtgcctcttg gatcagttgc gcggcggtat ctttggcgcc ttgcagtcca agtagttgcg
    10141 gaaaagtact cttattggac tcggcgtctg agccttgggg tttgccgagt tcttccgtgg
    10201 tggcaatgat gtccagaata tcgtcttgca cttggaaggc aagtcctacc gcatgggcaa
    10261 attccatcat agtctgatat tcatcttttg gcgcattagc ggcaatcaag gctaactcaa
    10321 cggcgcagct gataagggcg cccgttttca tattgtggag ttgagttagg gccttaaggt
    10381 tgatgagctt atcggttgag gctaagtcaa tcgcttggcc gccacacata ccacggtagc
    10441 ccgaggcttt cgccagcgct ctcaccatgg cgatatgttg ctcgctgctg atccctggta
    10501 ctgcttcggt aacaatttca aaggctaagg tttgcagagc atccccagca aggatggcgg
    10561 tcgcctcatc gaaggcgata tgcactgtgg gttgaccacg acgaaggtcg tcatcgtcca
    10621 tggcgggtaa atcgtcatgg attaacgaat aagcatgaat acactcaatg gcggcggcgc
    10681 aggcgtcgag cttttcaagc gacacgccaa acatttgccc cacactgtaa accaagaagg
    10741 gacgaatacg cttaccgcct aatagcgcgc cgtgggtcat ggcggccttt aattgtggcg
    10801 cagtgtcttc gagagtgctg aaacgttgtt ttaattggtc atcaacccgt agttgatatt
    10861 cttttattgc attgactaac acgcttattc gccctcaacg gcatagggag agaggggcgc
    10921 attttcatcc tgcttgagta aaatggccac tttttgctga gcttgctcga gttttgcttg
    10981 gctgttacgc accagattga tgccacgttc aaattgtttt aacgcatcat ccagagagac
    11041 atcgccctgt tctaattcgg caacgatgcg ctcgagttca ccaagggatt cttcaaaact
    11101 gagattttcg ggtttcttcg ccacgttaat aattcctttg aaagagcgga cacaaggtat
    11161 atcagggcga ctcaagggtc aaattcagtc gacggatatt tgctcaatag cacgctttgt
    11221 tgctgaattt tttcagaaaa ttgaaattgt cgctaaactt gcttagtgct tgtccgataa
    11281 atgacaataa tagcattatg cttataatca tcctttgaca tcatagagta aggctcaggt
    11341 taaaaccaaa gtgtaatccc tatgcggatg acattttgta ttattttgcg aatagagtca
    11401 gtatcatgtc gtttataccg aatattgagg agccgttgtg gatttagcta cagttatagg
    11461 actagtcggg ggctttggtt ttatcctttg ggctatgatg accagcggcg gcctggcaat
    11521 ctttatcgac gtcccctcag tgtttatcgt gttcggtggt tcattcttcg tcgtaatgat
    11581 gaaattcaat ctcaaacagt ttttgggggc ggtaaaaatc gccgtcaaag cctttatgtt
    11641 taagattgat aagcctgaag atcttatcga gcaatccgtg actatggcgg acgccgcccg
    11701 taaaggcgga ttccttgccc tcgaagaagc gcaaatttcc aacagtttta tgcaaaaagc
    11761 cgtcgatatg ttggtcgatg gtcacgatgg tgaagtggtg cgcgccgcac ttgaaaaaga
    11821 cattgccctt acagaagagc gccatcgcaa cggcattgcg atttttaggg cctttggtga
    11881 cgttggcccc gcgatgggga tgattggtac cttagtgggc ttggtggcca tgttatccaa
    11941 catgtcggat cccaaatcga ttggtccatc gatggcggtg gccttgttaa cgacgctgta
    12001 cggcgccgtg gtcgccaaca tggtgtgtat tcccattgcc gataaactca ccctacgcat
    12061 gggtgaagaa atgcttaacc gcaacctgat tatggatgcg gtattagcca ttcaagatgg
    12121 ccaaaaccca cgggtgattg aaggtttctt gaagaattat cttgctgaaa aacagcggaa
    12181 aatcgataca acggacggag agtagcccat ggctaagtgc aactgtccac cgcccggagc
    12241 accgctctgg ttggcaacat ttgccgattt gatgtccctc ttgatgtgtt tcttcgtgct
    12301 gctgctgtca ttctccgaaa tggacgtgat gaagtttaag cagatcgcag gctcgatgaa
    12361 gtatgccttc ggggtgcaaa acaaggtcga agtcaaagac attcctaagg ggacgtctgt
    12421 gattgcattg gagtttcgtc ctggtcgtcc cgatcccacg ccgattgaaa tcattaacca
    12481 acaaaccaac gaaatgaccg aacccgtatt ggattatcaa gcgggcgaag acgatagcgc
    12541 gggaggtgtg cagcagcaaa atggttcgca gcggggcggt gaagcctcgg cgacggccca
    12601 agaaacggcg gatgcggtga aggccgaagc cgcggcggcg caggataaaa tcaatcagca
    12661 agtgaagaaa atggcacagg aactcaataa tgagatcacc gatggggcga tcgaaattga
    12721 gtcacttggg cagcaaatca ttatccgtat ccgtgagaag ggctcatttg cctcaggatc
    12781 tggcttcctg cagccgcgat ttaaacccgt ggtgcgctcg gtcggcgaat tgctaaaaga
    12841 tgtgccgggg atcatcacgg tatcgggtta caccgatgat atgcagataa gtgatgagct
    12901 ctatagctcg aactgggatc tctccagtaa acgcgctgtg gcagtggccg atgtgctgct
    12961 gcaagtgaag ggcttcgatc ctaagcggat gaaagtggtc ggtatggcgt cgaataatcc
    13021 aatcgtgccc aatgactcac ctgaaaaccg tgcacgaaat cgtcgtgttg agattgccat
    13081 tgaacagggt aaagccaaag aatccgaaga aattcaagta ggtaaatagc ttttaatcca
    13141 aacctgtgat tcgataaaaa cgccgtgact tgctcacggc ttttttattt gtgatttttt
    13201 gactataagt tcaactgtgc ataagcgcac gccacgagca gcccgagtgt ggaaacaggt
    13261 gcgtcaatcg ccgtgagttt gttataatcc ggcgattatt cttttaacca attttgctgt
    13321 ttacaaaatt gcccgcggaa atcccatgaa gtttattgta aagctgtacc cagaaatcat
    13381 gatgaagagc aagcccgtgc gcatgcgctt caccaaaatg ctcgaaacca acatccgtaa
    13441 cgtgctcaaa aaagttgatg aagatgctaa agtgcaacgt caatgggacc gtatttgggt
    13501 aaaggtgcca aatgataaac ctgaattagc tctggccttt ggtgagcgtt tagcctgtat
    13561 tcctgggatc gcccatgtgg tgcaagtgga tgaatacagt tttacctctg ttgacgatat
    13621 ctaccaacaa gtcttacccg tatatcgcga ccaaattgcc ggtaaaacct tctgtgtgcg
    13681 cgtcaaacgt actggctccc acgattttaa ctctatcgaa gtcgagcgtt atgtcggtgg
    13741 tggtttaaac cagtttaccg atgcgattgg cgtgcgtttg aagaacccag aagtgacagt
    13801 taacctcgaa atcgagggcg ataaactgta tatggtgacc aagcgtatcg aaggcttagg
    13861 cggcttccca atggcgacac aggaagatgt gttgtccttg atttcgggcg gttttgactc
    13921 aggcgtgtcg agctaccaat ttattaagaa gggcgcacgt acccattact gtttcttcaa
    13981 cctcggtggc gcgcagcatg aaattggcgt gaaacaagtc gcttaccatt tgtggaaaac
    14041 ctatggtgaa tcccacaagg tgaagtttgt gtctgtgccg ttcgagcctg tagtggccga
    14101 gattttagag aaaatcgaca acggtcaaat gggcgtggtg ctcaagcgta tgatgatgcg
    14161 caccgcggcg cgtattgccg agcgtatggg tattcaggcg attgtgacag gcgagagttt
    14221 aggccaagta tcgagccaaa ccttaaccaa cttaaacgtg attgaccgct gcaccgatat
    14281 gctgatactg cgcccgctga tcgccatgga caagcaggac atcatcaacg aatgtcgccg
    14341 tattggcacc gaagattttg ctaaatctat gcctgaatat tgcggcgtga tttcgcaaaa
    14401 gccaaccgtt aaggcggtac tggccaaggt tgaggccgag gagactaaat tctctgagga
    14461 cttgattgac cgtatcgttg agcaggccgt ggccattgat atcagggaga tagcagaaca
    14521 aatgaatacg cgtatcactg aaactgaaac cgttgttgct atcgacacca cgaagtggtg
    14581 attgatattc gcgccccaga ggaagaagag aacaagccgc tggagattga aggcgtggaa
    14641 atcaagcgca ttcctttctt caaattagcg actcagtttg ccgatctcga taagcagaag
    14701 acttacctgt tgtactgtga gcgtggtgtg atgagtaaat tacaggcgct atacctgatt
    14761 gagcaaggtt atcacaatgt taaggtttac cgcccttaag cgataagcag taatgcacgt
    14821 gcgaaggcca aacgtcaatt ttggccttaa tgcgcatgaa ttggggcctt aatgcgccaa
    14881 taagcaggta aacgaataca aaaaagccgc attggaatgc ggcttttgtt ttactgttca
    14941 gcagtgcaat ttattcggtc ttcaccactt gctgggccgg taacacttca tcggcaagca
    15001 ataaggtagg atcctgcagc gcttcttgat gcatttcttc catcatcgca gtcagttcgg
    15061 cattgatgct tgcctgtaaa tcactggtgt cgatgataat ttctgcatcg ataacatttg
    15121 cgctgacatt ggcactgagt gcgctgacta acactaacgc aaaagcagat tttaaattga
    15181 acatagtcgc ctccgggctg acaaagtatt gctgtcagac attcccatca cccgttcgag
    15241 attgtccaac tcatgtgttt cgttggcgca aaatttaatc aaaaacgctg gcctttacaa
    15301 acgattaaat gtaacaatac ggattagaaa aactaattaa aaattgagat agagatcact
    15361 atgtcaagac gcttaccacc actgaatgcg gttaaagcat ttgaggccgc cgcacggcat
    15421 ttgagcttta ctcgagctgc tgaagaattg tttgtgactc aggctgcggt gagtcaccaa
    15481 attaaggcgc tggaagattt tctcggatta aaattatttc gccgaaagaa tcgttcgctg
    15541 ctgctgaccg aagaaggtca gagctatttt ctcgatatta aagatatctt cactcaactg
    15601 gcggatgcga ccgacaggct gctcgcacgt agcgccattg gttcgttgac cgtgagtatg
    15661 tcgcccagtt ttgcgattca gtggctggtg ccgcgcctgt cgaagtttag tgaaaaaaat
    15721 cccgacattg acgtacgaat taaagcggta gacacagagg ccagctcctt aaccgacgat
    15781 gtcgacgtcg ccatttatta tggtttaggc aattggccgg gactgcgcgc cgataaactc
    15841 cgcaatgaag tgttgatccc cgtatgttcg cccatgctgt taaatggccc taagccgtta
    15901 tcaaagccgg aagatttaaa ataccacacc ctgctgcacg atatgagccg ccacgattgg
    15961 caggcttggt ttagacaatg tgggataagt gatatcaacg tcaaccaagg gccgattttc
    16021 agtcattctt ccctcgtctt acaggcggcg gcccacggcc aaggtgtggc cttaggttat
    16081 agcgtgttgg ctcgccccga tatcaaggcg gggcgactgg tgtgcccatt ccaagaggtg
    16141 ttagtgagta aagatgctta ttacctcgtc tgccaacaga atcatgccga gctggggaaa
    16201 gtggtcgcgt ttcgtgagtg gatgttagat atgtttgccg aggagtcacg cagtgagttg
    16261 ttgcccggat aatcccttga ccaacgagga tgttgcttca agcgcctatg tgttagcagg
    16321 cgagccatca gaaaccttga ttttatttgc ccacggcgca ggagcgaatc gagactccga
    16381 ctttatgtgc caaatggccg caggtcttgt cgccaagggt tttcaagtga tgcgttttaa
    16441 ctttccctat atgcaggcca atgccgtgga tggtaaaaag cgcccccaga tagagcgcca
    16501 aagttgcttg cctgttttag cgagatgctc gatgtggcgc acgctcaacc taaggttaag
    16561 cgtgtggtct tgatgggcaa atccatgggc ggacgcatgg cggctttatt ggcctgcgat
    16621 agtgtgcaag cgagccgtat cgacagcgtc atttgtttag gttatccctt tattccgctt
    16681 aagggcggtg aacctaggtt agaatcccta aacgactgtc aagtgccggt gttagtgctc
    16741 caaggggagc gggataagtt tggcggtaag atgcaaatcc ctagctggcc acttaacagt
    16801 gatattcaaa ttgaatactt agccgatggg gatcatagtt ttgtcccaag aaagtcgtca
    16861 ggcacgaccg aagcggctaa ccttgcattg gctgttgatt tatcagaaaa atttattggt
    16921 tagtgcttat tcttaatgtg acctaaagcg ttcaggcatg actgctagag cgtcgtctgt
    16981 cgtaaagcct attggttttt ggaggtaaaa atgcgtaaag gattgttgtt actcgccgca
    17041 gtaagcggat ttttgcggtc gcgctaggcg cctttggcgc ccatggactc aaggctgtcg
    17101 cgccgcctga gctgattgag gtgtttaatc tgggggtgca atatcacttc taccatacct
    17161 ttgcgctgat tgccgtggcc tttgccggac agtggttaac ctcgcgtttg ctggactggg
    17221 cggcgtacct gtttatggcg ggcatagtgc tgttttctgg ctcgctttac ggtttggcgc
    17281 tggtcggtag caaatggttt ggccctatca ccccgatggg cggtggctgt ttcctcttag
    17341 gttggttatt gctggccgca gccgtatggc gccataaagt ggtggatggt aacgagtcat
    17401 aatgctcgta tggcgtagcc actgcgccat gggggcatcc agccaatcac acaaaagccc
    17461 gatagcaaat cgggcttttg tgtattgaaa ttgctatact ggcgcccaaa tcgatagtcg
    17521 gccatgttta tggcttggat ttcttaagga tctccatgaa aaacctattt ttattttgcc
    17581 gtgcaggcta tgagaaagaa tgcgcagcag aaatccaaca gcgagccgcc gagctcaatg
    17641 tgggtggctt tgttaaggcc aacaacaacg atgcctacgt ggtatatcaa tgttttgaag
    17701 aagacggcgg cgatacctta gtgaagcaac tgccgttgga ttcactgatt tttgcccgtc
    17761 agatgtttgc cgcaagcgag ttgttggcgg atttacccga gtctgatcgt gtcagcccga
    17821 ttgtggcggc gctcagtgac gtgagtaaag cgggtgagct gagggttgaa acccctgaca
    17881 ccaacgaagc gaaagaactg tctgcatttt gccgtaagtt taccgtgcct ttgcgccagc
    17941 atttgaaaaa atccggcagt ttactggcgc aagagaatcc taagcgtccg attattcacg
    18001 tctgttttat cggcccaggt cgcgcctacg caggttattc cttcagcaac aacagctcac
    18061 cctattttat ggggatcccg cgcctgaaaa tggctgccga tgcgccaagt cgctcaagct
    18121 tgaaactcga tgaagcgttc gcgcagtttg tgcctaaaga agaacaggaa gaacgggttc
    18181 gcagcggcat gaatgcggtg gacttaggcg cttgccctgg cggctggacc tatcagttag
    18241 tgcgtcgtgg catgatggtc tccgcggtcg ataatggccc aatgaacgaa aagctgatgg
    18301 agaccggaca agtgaaacat ttccgtgagg atggtttccg cttcgagcca cagcgtaaaa
    18361 acatttattg gttagtgtgt gacatggttg aaaaacctgc gcgcgtggct gagttgattg
    18421 aagcttgggc catcaatggc tggtttaaag aagcgatttt caacctcaag ctgccgatga
    18481 agagtcgtta taaagaagtg atggcgattt tgaataccat gcaggaaatc ctcaaggaaa
    18541 acggcatcaa cgagttccag ttgcagtgta agcacttata ccatgaccgt gatgaagtga
    18601 ctgttcacct gtggattaga ccgagccagg cttggaacta atctgcggtt tttcaccgtt
    18661 cttttcgtga ctgaagcccc aagtgatgtt tggggctttt tgtttggctg tcccactata
    18721 tagcggccat aaaaaaacgc acccaaggtg cgttttttgt ttcgcgcaaa gcgattatga
    18781 cagacggtca agcaccgctt gagtgaagtc tgtcgtgccg tgggtgccgc ctaaatcgcg
    18841 agtcgtacgg tccccttctt caatcactgc cgatacggct ttacggatca aatcggcttt
    18901 atcagccatg cctaagtatt ccagcatttg gatagaggct aagatcactg atgttgggtt
    18961 cgcgaggttt ttacctgcga tatctggcgc actgccgtga accgcttcaa aaatcgctgc
    19021 atcacggcca atgttggcgc cgggtgccat acctaaacca ccgactaaac cagcacacag
    19081 atctgacaga atgtcaccga acaggttggt ggtcacgata acgtcgaagt tttctgggtt
    19141 catcactaac ttcatgcaag tcgcatcgac gatcatttct tcggttttga tgtctgggta
    19201 gcgttggctc acttcacggg ccactttcag gaacagacct gaagttgact tcatgatgtt
    19261 tgctttgtgc acgatagtga ctttcttacg gttttctttg cgagccagtt cgtaggcgaa
    19321 tgtcgcgatt tgctcagcac cttggcgagt gacgatactg gtcgcttctg cggtggtgcc
    19381 gtcttcagac actttttgac cgtggcctga gtacatgcct tcggtgtttt cacgcactgt
    19441 gatgatatca atgttttcat aacgggcttg agtgcctttg aaagacaaca ctggacgcac
    19501 gttagcgtac aggccgaatt tcttacgtaa tgtcacgttg atagaggtga agccttcgcc
    19561 tactggtgtc gtcagcgggc ctttcagggt gatacggttt ttttcgatca gttctaaagt
    19621 acgctgcgga agtagttcgc cctgcttctc gagagcagtt aagccggcat cggcaaattc
    19681 atattcaaaa tcacaacctg ctttatcaag aatttttaag gctgaatcaa taatacttgg
    19741 gccaattcca tcacctggaa ttacggttat cgttcttttt gacatggaga agtccttcca
    19801 cggttcgtcg ttactgcaat tgtccgaccc gggattagcc gggtaacatg actatgaatt
    19861 tggtgttttt tctccccatt attttaaccg tctttggtca aacttcacag gaaatagtga
    19921 gcaatatcac gattttttat tgggtaaaac ttgtaaaaat actgggttat aacgatgatg
    19981 gagagccaat ggttaaaaaa gtctacttta gtctaacaac tacataaaag aggaagacat
    20041 tgaagttatt acctatcaca tcgctagtgc ttttatccct cggaatactg cccaatgccc
    20101 atgcagaaac cagcactaaa ccgcttaccc tgaccgacat tatgcatttt gagtccctcg
    20161 aaaaacccgt tatcgctgac aatggtcaag tgctcgcggt ggaggtggcg ccagatcgtg
    20221 gcgatagcca tggtttgatt aagaatctac aaacaggtaa cgcgtttcag attgaaggtg
    20281 gctcggatcc tatcgtgagt cacacaggcc gttacgtagc gatggcggtt aaacccagtt
    20341 tactcaccgt tgaaaccagc gatgctaaag cgaagaaaaa gcttaaggcc gatatggtgc
    20401 tgctcgacac tgaaacgggt acgcaaacca aatttgaacg ggtaaaagaa tttgtcttta
    20461 gcgacgatgg cgcacattta gccatttggt ttgaagccga cgaagaggcc aagaaagacg
    20521 ccaaagagaa aaccgaaggc attgatgata agcccaaagc cgagaaggtg aaggtcgata
    20581 agttcgacca aggccgtcgt ttgagtttag tgaatttgtc ttcgccatcg cagcgggtgg
    20641 atctggagca tgtgaccgcc tatcagtttg ataaagacag tcgccgcttg gctatcgctg
    20701 tcaatgatat cgaggcgaaa caacatcaac tgcaactgat tgatttgaaa gactacaaaa
    20761 aatcaacggc atttgaatct caaactcagc aaatcggtgc ggtttcactg tcgaaaaatg
    20821 gtcgctggct ggcctttact caaggggatg cgagtgaatt accctatggc cgcagctatc
    20881 ag
//
