LOCUS       NZ_CP104864            10216 bp    DNA     linear   CON 13-OCT-2024
DEFINITION  Streptomyces lusitanus strain FZ202 chromosome.
ACCESSION   NZ_CP104864
VERSION     NZ_CP104864.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lusitanus
  ORGANISM  Streptomyces lusitanus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Streptomyces lusitanus FZ202
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.8x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025427835.1-RS_2024_10_11
            Annotation Date                   :: 10/11/2024 19:56:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,707
            CDSs (total)                      :: 6,615
            Genes (coding)                    :: 6,460
            CDSs (with protein)               :: 6,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 88 of 155
            Pseudo Genes (internal stop)      :: 3 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:00:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6973030
            Orig. end                         :: 6983246
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei,  College of Life
            Science and Technology.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="20"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1552..1565)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.03"
                     /tool="antismash"
     gene            complement(1855..4077)
                     /locus_tag="N7E53_RS31555"
     CDS             complement(1855..4077)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="N7E53_RS31555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_033275281.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-38, bitscore:
                     130.3, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRGRRRAAPPRRKVPKSRAKTVRTPTVLQMEAVECGAAS
                     LAMVLGHHGRHVPLEELRIACGVSRDGSRASNLLKAARSYGLTAKGMQMDLAALAEVTS
                     PAILFWEFNHYVVYDGMGRRFGRRGVYVNDPAKGRRFVPLEEFDGSFTGVVLVMEPGDD
                     FTRGGRKPGVLGAMPARLRGTAGTMPAVVLTSLLLVAVGAALPGLSRTFIDEFLIGQQT
                     SMLDVLFASMGACVLLTLLLTWLQQANLLRGRLISSTLSSARFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLSAAGVDAVVVVLYAVLLYTYDPQLTFVGIGVALLNIL
                     AMRIVIRLRATRTAKLRADTARLTNTAYTGLQLIETLKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGVVAPTLATLNSALILWIGGMRAVEGHISVGLLVAFQALVARFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFRTDPLYGRPGAGESTRRLHGHVELQNLTFGYNP
                     LDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYTPWEGVIRIDGQRIEDIPR
                     GALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDEAVVEALKDAALYDVIVRRPGGIHS
                     PVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRRRGCACV
                     VIAHRLSTVRDSDEIVVLDQGTVVERGRHEELVARGGAYAALVRER"
     gene            complement(4074..4880)
                     /locus_tag="N7E53_RS31560"
     CDS             complement(4074..4880)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="N7E53_RS31560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_033275280.1"
                     /transl_table=11
                     /translation="MQFRQQALAKLQSPEDLDLPVRFARPQGWLVLSVTVIAMAAASVW
                     ALTGSVTSTVQAPAILTYGQGSYILQSPVAGQVTAVLAREGERLPANSPVLKVRTPDGD
                     QVVRTLQAGRITALAASAGQIISTGADVAAVEKVRGDEPPLYATVYVPAQKAAGIPAHA
                     SVDLTVQSVPSQEYGVLRGEVKKVDRSAQSPRQIAAFLGDTQLGEEFTRDGRPVAVTVK
                     LKRSSGTESGYAWSSADGPPFELTSMTLATGSIRLSDQRPVDWLLP"
     gene            complement(5001..5216)
                     /locus_tag="N7E53_RS31565"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="N7E53_RS31565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_033275279.1"
                     /sec_met_domain="strepbact (E-value: 1.6e-35, bitscore:
                     120.1, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEIADAELDAVSGGLSVNAVNTLTGALDGIAPVSGL
                     VDTVVGTVEGVTGLNTAPVTNLVAGL"
     misc_feature    5232..5245
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.44"
                     /tool="antismash"
     gene            5787..7148
                     /locus_tag="N7E53_RS31570"
     CDS             5787..7148
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994899.1"
                     /locus_tag="N7E53_RS31570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S1 family peptidase"
                     /protein_id="WP_261676095.1"
                     /transl_table=11
                     /translation="MSHKRIPKRKAVMAVGGVVALGAAAILLPNANASQDGASDGVTAA
                     PRTLKAGDASDLATRLAGTLGDAWAGAWYDGEDKQLVVNVVRGENTMALKVRSAGAEVR
                     WVDNSMAELEAGAGTLKSEATIPGTSWAVDPRTNKILVTADSTVTGDQWDQLESTVEDL
                     GSGMATLKKSAGTFRTFASGGDAIFAGGARCSLGFNVTAGDGSPAFLTAGHCAAAGDEW
                     SDQQGGRPIATVDQALFPGAGDFALVTYDDPATEAPSEVNVGGQTVPIVQAAEATVGQQ
                     VFRMGSTTGLADGQVLGLNATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTQDGLAIGLT
                     SGGSGDCTVGGETFFQPVTTALEAVGATLGDGGAGAGDQAGGQEQGTGQEAGDGQGGAE
                     DQAGAGDQAGAGDQTGGQDAGAGQDGAGGQDMRDGTGTGSGRHSGHDSGLSRTH"
     gene            complement(7594..9726)
                     /locus_tag="N7E53_RS31575"
     CDS             complement(7594..9726)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430712.1"
                     /locus_tag="N7E53_RS31575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_033275447.1"
                     /transl_table=11
                     /translation="MSEGQASARSHAPVGRPLLSLTLAALMDEVHAHSGAVYLLSPDQP
                     VLEMAVMAGLPRAFAAPWERVGLSAPIPVSDAVRERRLVWVGGGEEMARRYPRIAMVLP
                     YPFCLAALPVATAAKTYGAVFVTWPGSHPPELSVRERERLTAACDRLAVRLERAAERSL
                     PLAHEPDLLAAPVVGGAAGSLGTVEAARMVSRLPYGLCSLDLHGRVAFANAAASDLLGV
                     APDQLSGAHLWAAVPWLNDPVYEDRYRAALLSQHVTSFVALRPPDTWLSFRLYPSTTGL
                     SLRVSRARAVTEMNRAAPQPGAGRGRLVTITPVLGLAGALTEAVGVQDVVQLVADEIVP
                     AVGGQGLVVLGSRAGRLRVLGHRGYADPLVVERFDGLPLSEPTPGGQVLRTGVPAFFES
                     REELERLYPALQTHPSEFSAWAYLPLIASGRPVGTCVLSYGAPHRFATEERAVLTSLAG
                     LIAQALERAMLYDVKHRLAHGLQEALLPHTLPALEGIEAAARYLPATQGMEIGGDFYDL
                     VPVDGVAAAVIGDVQGHNVTAAGLMGQVRTAVRAYTAVGQAPDEVMRSTNRLLLDLGAE
                     LFASCLYLRLDAARGRAVMSRAGHPPPLLRRPDGRVRVLDLAGGPLLGIDGTAAYPSTE
                     VALPPGSLLVLYTDGLVESPGVDLEDALAELCRRLTELGDLPLDDLADALMRHGPHSAD
                     RLDDTAVLLLRSLPRP"
     misc_feature    9702..9721
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.48"
                     /tool="antismash"
ORIGIN
        1 accgccgtca ggttcttgat ccgcccggcc ttctgctgga gctcacggct gcgcgcgaac
       61 tgcgacgccc acgccgcgta cgcgtagttc tccgccgccg ccacccgcag cttcggcagt
      121 ccgcgcaggg tctggaacgc ctggttgttc agcttgttgg tgagcaccac cagccgtcgc
      181 tgccagcgca cctgccacag cccgagaccc aggaacacgg ccgcgatcac caccagcatg
      241 cccatcgcgg ccagcgccat cggcacgctg aaccacagca gcagcgccag gttcaccgcg
      301 cccaccgtga gcgactgcgt caccgtgggc ccgacaccgg ccagcatgcg gcggatcgcg
      361 ctgatgccca tcgccgcgct cgccagctcg cccgtggacc gctccgtgaa gaaccgggtc
      421 ggcagcctga gcagcctgtc ccacaccgcc ggctggagcg tggcctccac ccggccctcg
      481 agacgcagca gcgtgaggtt ctgcaacagc atgaacgcgg ccgcgaccac actgctgagg
      541 atcaccgcga gacacacctg cacgatcagg tccgtctgcg ccttcggcac gtactcgccg
      601 agcacccggc ccgtcgccag cggcaccagc gcccccagca ccaccgtcac cagaccgctc
      661 agcagcaacc ccgtcagatc gccccgggtg ccgcccatgc agaaccgcat cagccgcagc
      721 gggctcagcc gccgttcggg cagcggacgg tagaacatca ccgcgcgcgg ctcgaactcc
      781 tcggcgttcg ccttctccac cggggtctcc cggcccgtcg acggatgcac ggccacatag
      841 ccgccgcgcc gccacagcag cgccaccggc gcccccgaca gggcacggtg acccaccagc
      901 ggtcccacgt cgtcgcgcca ccaccggccc tccagccgca ccacccgggt gcgcagccgc
      961 gaggccagcg ccacctgctc caccgggtcc agccggtcgg cgccggtgcc ggactgcgcc
     1021 ggcgaggcca gtgtgatccc ggccgcccgc gccaccagct cgcacgccgc gtacgtggcg
     1081 tccgcgtccg cggccgtcgt gcgctgcccc gaccgcttgc cgatcgacgc gagcagcgtc
     1141 cggtcggcct gcgcccgtac cgcctcaccg gccgcgatgc cctgcgcggt gcgcgactcg
     1201 tgcgtctcct ccagctgctc gatccaccgg tccagcgtgg tcatcagccg gtactgctgg
     1261 tcgaccatgc tctgccacag cgccgggtcc atcagcaggt cggccgcggc ctccgcgccg
     1321 tacacggagc cgtactgcac gctgcccggg ggcacctgca tccagaacac gtcgtcgtcg
     1381 gtgggcgccg ccgcccgctc cgtcgccatc ggcgcctgga acagcacggc gaggctccgg
     1441 cccacgccca gcgcgagcgc gtactccagc gggctcgtcg tgggcggcac gtactgcggg
     1501 ttgccgtacg cgtcgtacga ccaggtctgg gtctgtgcct cctggtacag ctcacgcagc
     1561 gcgatgcggt gcaccacgca gtcccgcagc ggccgcgcca ccagcgtgtg cgccggaccc
     1621 gccaccggac ccagcaccag cgaacccgcc tccagacggc ccaggtggtg ccagtgcccc
     1681 tgctgctccg cgtccaccgc gaacaggtcc acggcgccgg acgcgatcag ccacagcacc
     1741 tgcggcccct ccaggtccag cttcccgaac ccggagcagt cgatcggggt gcccagcgcg
     1801 ccgagcgcgc ccaggacgac gtcgccgccc tgcccctcgt gcacggcagt catctcatcg
     1861 ctccctgacc agcgccgcgt acgcgccgcc gcgcgccacc agctcctcgt gccgcccgcg
     1921 ctccacgacc gtgccctggt ccagcacgac gatctcgtcg ctgtcgcgga ccgtggacag
     1981 ccggtgcgcg atcaccacgc acgcgcagcc gcggcggcgc aggttgtcca tcaccaccag
     2041 ctccgtctcg gcgtccagcg cgctggtcac ctcgtcgagc accaggatgc tcggccggcg
     2101 caccagcgcc cgggcgatct ccagacgctg gcgctggccg ccggagaagt tccggccgtc
     2161 ctgctcgacc gggctgtgga tgccgcccgg acggcgcacg atcacgtcgt acagcgccgc
     2221 gtccttcagc gcctccacca ccgcctcgtc cgggatcgac gggtcccaca gcgccacgtt
     2281 gtcccgcacc gtcccctcga acaggaacac gtcctggtcg acgaaggaga cggacgccgc
     2341 cagcgccccg cgtgggatgt cctcgatgcg ctgcccgtcg atccggatga cgccctccca
     2401 cggggtgtag agccccgaga tcagccgtga cacggtcgac ttgccgctgc ccgagccgcc
     2461 caccagcgcc acctgctgcc ccggaccgac cgtcaggtcg aagccggtca gcagcggctt
     2521 gtccagcggg ttgtagccga aggtgaggtt ctgcagctcc acatggccgt gcagccgccg
     2581 ggtcgactca ccggcgcccg gacgcccgta cagcgggtcc gtccggaagt tctcgacgtc
     2641 cttcagccgg gccacgtcgg cggcgaagtc ctggatgcgg cccgccaccc cgttcagccg
     2701 ggtcagcggg gccgtgaacc gggcgaccag cgcctggaag gccaccagca gaccgaccga
     2761 gatgtgcccc tcgaccgccc gcatgccgcc gatccagagg atcagcgcgc tgttcagcgt
     2821 ggccagcgtc ggcgcgacga cgccgagcca cgcgctcggc accccgagcc gctgctgctc
     2881 ctccagcgtg gtggcgtgct gcccggccca cttgcggaag tagccgtcct cgccgccggt
     2941 cgccttcagc gtctcgatca gctggagccc cgtgtacgcg gtgttggtga gccgcgccgt
     3001 gtccgcgcgc agcttcgccg tgcgggtggc gcgcagccgg atcacgatcc gcatcgcgag
     3061 gatgttcagc agcgccaccc cgatgcccac gaacgtgagc tgcgggtcgt acgtgtagag
     3121 cagcaccgcg tacagcacga ccacgaccgc gtcgacgccc gccgccgaca ggtcacgggc
     3181 cagcgtctcg gccacggcgt cgttggactg caggcgctgc acgaggtcgg cggggctgcg
     3241 ctgggagaag aacgtcaccg gcagccgcag caggtggcgc aggaagcgcg cgctggacag
     3301 cgtcgacgag atcagccggc cgcgcagcag gttcgcctgc tggagccagg tcagcagcag
     3361 cgtcagcagg acgcacgcgc ccatcgacgc gaacagcacg tccagcatcg acgtctgctg
     3421 cccgatcagg aactcgtcga tgaaggtgcg gctcaggccg ggcagcgccg cgcccaccgc
     3481 caccagcagc agactggtca gcaccacggc gggcatcgtg cccgcggtgc cgcgcagccg
     3541 cgccggcatc gcgccgagca caccgggctt gcggccgccc cgggtgaagt cgtcgccggg
     3601 ctccatcacc agcaccacgc cggtgaagct gccgtcgaac tcttccagcg gcacgaacct
     3661 gcggcccttg gcggggtcgt tgacgtacac cccgcgacgg ccgaagcggc ggcccatgcc
     3721 gtcgtagacg acgtagtggt tgaactccca gaacaggatg gccggcgacg tcacctcggc
     3781 gagggcggcc aggtccatct gcatgccctt ggcggtcagc ccgtagctgc gggccgcctt
     3841 cagcaggttg ctggcgcgcg agccgtcccg cgagacgccg cacgcgatgc gcagctcctc
     3901 cagcgggacg tggcggccgt gatggcccag caccatggcc agcgaggcgg ccccgcactc
     3961 cacggcctcc atctggagca cggtgggcgt acggaccgtc ttcgcgcggg acttgggcac
     4021 cttacggcgg ggcggggcgg cgcggcgtct gccgcgggtc tcctgtgcgg tgctcacggc
     4081 agcagccaat ccacggggcg ctggtcggac agccggatgg aaccggtggc cagggtcatc
     4141 gacgtcagct cgaacggcgg gccgtcggcc gaggaccacg cgtagccgct ctccgtaccg
     4201 gacgagcgct tgagcttcac ggtgaccgcc acgggcctgc cgtcgcgggt gaactcctcg
     4261 ccgagctggg tgtcgccgag gaacgcggcg atctggcgcg gggactgggc ggagcggtcc
     4321 accttcttca cctcgccgcg cagcacgccg tactcctggc tcggcaccga ctgcaccgtc
     4381 aggtcgacgg aggcgtgcgc ggggatgccg gcggccttct gcgcggggac gtacaccgtg
     4441 gcgtacaagg ggggttcgtc gccgcggacc ttctcgacgg cggcgacgtc cgcgccggtc
     4501 gagatgatct gcccggcgct ggcggcgagc gcggtgatcc ggcccgcctg aagcgtgcgc
     4561 acgacctggt cgccgtccgg tgtacggacc ttgagcacgg gggagttcgc cggcagccgc
     4621 tcgccctcgc gcgcgagtac ggcggtgacc tgaccggcga cggggctctg caggatgtaa
     4681 ctgccctgcc cgtacgtgag gatggcgggc gcctgcaccg tggaggtgac cgagccggtc
     4741 agcgcccaca cggacgcggc ggccatcgcg atcacggtga cggacagcac cagccagccc
     4801 tgcgggcggg cgaaccgcac cggcaggtcg agatcctccg gcgactggag cttggcgagg
     4861 gcctgttggc ggaattgcac gggtcttccc tcacctgggt gaactgtgcg acgtgacgga
     4921 aggactggaa ggaccgcgaa cgcaccacag agccccggag ccggggtacg gctccgggac
     4981 tcggtggaac aagggcgcga tcagagaccg gcgaccaggt tggtgaccgg ggccgtgttc
     5041 aggccggtga caccctcgac ggtgccgaca acggtgtcga ccaggccgga aaccggggcg
     5101 atgccgtcca gggcgccggt gagggtgttc acggcgttga cggacagacc accggaaacg
     5161 gcgtcgagct cggcgtcggc gatctcgacg gtctcgacct ggggggtgga gttcatgatg
     5221 gaacttccct tcgtatggac tttcacaagg ggggagcggc ccccctctgg ggacagatga
     5281 aggccgcgac cgcgggcgct ccgagcgccc gtcgccggga gcccggaacg gccttgcggt
     5341 gcgatggatc aaagcacgcg gcaaccggcc gcttccaatt cctcacgcgc ctcaccaggc
     5401 aacttgcgcc caggcggcac ccaagcgtgc agacgagggc acggctcggc ggcgacttct
     5461 tcacatgggc cacggcatgt acccatccgg ccacttgcgg cccgtggccg gaaagcgaca
     5521 caccggccgc aatgacgtgt cccggatagg tgccctctgc atgtgtcatg ggtccggtga
     5581 ccgggttggg cattcgctgt gcagattcgc cgaagccggg attcaagacc cgagcggaca
     5641 cccggtgtgc ctgcccggtg gcggaacgtg agcgcccgga aaggcgttga acactcctca
     5701 actcccttgc gtaccaacga ccgtccaggc gccgaaccgc cgccggagag cggcacgact
     5761 ccggtccccc caggaggtcg agaagtttga gtcacaagcg aatcccgaag cgcaaggccg
     5821 tgatggcggt gggaggtgtg gtggccctcg gcgccgccgc gatcctgctc cccaacgcca
     5881 acgcgtccca ggacggcgcg tcggacggcg tcacggccgc gccgaggaca ctgaaggcgg
     5941 gcgacgcatc ggacctcgcc acccggctcg ccggcacgct cggcgacgcc tgggcgggcg
     6001 cctggtacga cggcgaggac aagcagctcg tcgtcaacgt ggtacgcggc gagaacacca
     6061 tggccctgaa ggtccgcagc gcgggcgcgg aggtccgctg ggtcgacaac agcatggccg
     6121 aactcgaggc cggggcaggc acgttgaagt cggaggcgac catcccgggc acctcctggg
     6181 cggtcgaccc gcgcaccaac aagatcctcg tcaccgcgga cagcacggtc accggggacc
     6241 agtgggacca gctggagtcc accgtcgagg acctcggctc cggcatggcc accctcaaga
     6301 agtccgcggg caccttccgt accttcgcgt ccggcggcga cgccatcttc gcgggcgggg
     6361 cgcgctgttc gctcggcttc aacgtcaccg cgggcgacgg cagcccggcg ttcctcaccg
     6421 ccggacactg cgccgccgcc ggcgacgagt ggtccgacca acagggtggc cggcccatcg
     6481 ccacggtcga ccaggcgctg ttccccggcg cgggcgactt cgcgctcgtc acctacgacg
     6541 acccggccac cgaggccccg agcgaggtga acgtcggcgg ccagacggtc ccgatcgtcc
     6601 aggccgccga ggccaccgtc ggccagcagg tgttccgcat gggcagcacc accggcctcg
     6661 ccgacggcca ggtgctcggc ctgaacgcca ccgtgaacta ccccgagggc acggtgaccg
     6721 gcctcatcca gaccgacgtg tgcgcggagc ccggcgacag cggcggctcc ctcttcaccc
     6781 aggacggtct ggccatcggc ctgacctccg gcggcagcgg cgactgcacg gtgggcggcg
     6841 agacgttctt ccagcccgtc accaccgccc tcgaggccgt cggcgcgacc ctcggcgacg
     6901 gcggtgcggg cgccggggac caggcgggcg gccaggagca gggcacgggc caggaggccg
     6961 gcgacggcca gggcggcgcg gaggaccagg cgggcgccgg cgaccaggcc ggagccgggg
     7021 accagacggg cggccaggac gcgggcgccg ggcaggacgg cgcgggcggg caggacatgc
     7081 gggacgggac cggtaccggt tccggccgtc actccggtca cgactccgga ctgtcccgga
     7141 cgcactgacc cgcgcgcgcc accaccgtgc gcgtgaggcg cccccggccg tgtgcgggcc
     7201 gggggcgcgc ccctgccgcg ggcagtcgtg ccgcttgggg cgatgggggg agggtgggcg
     7261 cagtggcacc cttcagcgcc gggtgcgggt cgtccgtggc tggtcgcgca gttccccgcg
     7321 cccctgaggt gtgcttccgc cacggccgtt ggaagcgccc tgttgtcgct gggggccggc
     7381 cctctcccgc gccgggtacc ggtggttcgt ggctggtcgc gcagttcccc gcgcccctga
     7441 ggcgtgcttc cgccagggcc gttgaaagcg ccccgtcggg ggtatctccc gctcgagcga
     7501 agccgggagt cgggaagggt gggcggcgca gcggcaccct gtcagcgtcg ggtgcgggtc
     7561 gtccgtggct ggtcgcgcag ttccccgcgt ccctcagggg cgcggcaggg acctcagcag
     7621 cagcaccgcc gtgtcgtcca gccggtccgc cgagtgcggg ccgtgccgca tcagcgcgtc
     7681 cgccaggtcg tccagcggca ggtcaccgag ctcggtgagc ctgcggcaca gctcggcgag
     7741 ggcgtcctcc aggtcgacgc ccggcgactc caccaggccg tccgtgtaca ggacgagcag
     7801 cgatccgggc ggaagcgcca cctccgtcga cggataggcc gccgtgccgt cgatgcccag
     7861 cagcggccca ccggccaggt ccaggacccg caccctcccg tcggggcggc gcagcagcgg
     7921 cggcggatgc cccgcccgcg acatcacggc ccgcccccgc gccgcgtcga gccgcaggta
     7981 cagacagctc gcgaacagct ccgcgcccag gtccagcagc agccggttgg tgctgcgcat
     8041 cacctcgtcc ggcgcctgac ccaccgccgt gtacgcccgc accgccgtac ggacctggcc
     8101 catcagcccg gccgccgtca cgttgtgtcc ctgcacgtcc ccgatcaccg ccgccgccac
     8161 cccgtcgacc ggcaccaggt cgtagaagtc gccgccgatc tccatgccct gggtggcggg
     8221 caggtaccgg gcggcggcct cgatgccctc cagcgccggc agggtgtgcg gcagcagcgc
     8281 ctcctgcagt ccgtgcgcca gccggtgctt cacgtcgtag agcatcgccc gctccagcgc
     8341 ttgggcgatc agccccgcca gactcgtcag caccgcccgc tcctccgtcg cgaaccggtg
     8401 cggcgccccg tacgacagca cgcacgtccc caccgggcgg cccgaggcga tcagcggcag
     8461 gtacgcccac gccgagaact ccgacggatg cgtctgcagc gccgggtaca gccgctccag
     8521 ctcctcccgc gactcgaaga aggcgggcac cccggtccgc agcacctgac cgcccggcgt
     8581 cggctcggac agcggcagtc cgtcgaaccg ctccaccacc agcgggtccg cgtacccgcg
     8641 gtgcccgagc acgcgcagcc ggcccgcacg ggacccgagg accaccagcc cctggccacc
     8701 caccgccggc acgatctcgt cggcgaccag ctgcaccacg tcctgcaccc cgaccgcctc
     8761 ggtcagcgcc ccggccaggc cgagcaccgg ggtgatggtc accagccggc ccctgcccgc
     8821 acccggctgc ggcgccgcgc ggttcatctc cgtcaccgcg cgggccctgc tgacccgcag
     8881 gctgagaccg gtggtgctcg ggtacagccg gaaggacagc cacgtgtcgg gcggccgcag
     8941 cgccacgaac gacgtgacgt gctggctgag cagcgccgcc cggtaccggt cctcgtacac
     9001 cgggtcgttc agccacggca ccgccgccca cagatgcgcg ccggagagct ggtccggcgc
     9061 cacgccgagc aggtcggacg cggcggcgtt ggcgaacgcg acccgtccgt gcaggtccag
     9121 cgagcacagc ccgtacggca gccgggacac catccgggcc gcctccaccg tgcccagcga
     9181 gccggccgcc ccgcccacca ccggcgcggc cagcaggtcc ggctcgtgcg cgaggggcag
     9241 gctgcgctcg gcggcccgct ccagccgcac cgccagccgg tcgcaggcgg cggtgagacg
     9301 ctcgcgttcc cggaccgaca gctccggagg gtgggagccc ggccaggtga cgaagaccgc
     9361 gccgtacgtc ttcgccgccg tcgccaccgg gagcgcggcc aggcagaagg ggtaggggag
     9421 gaccatggcg atccgggggt agcgacgggc catctcctcc ccgccgccca cccacaccag
     9481 ccggcgttcg cgcaccgcgt cggagaccgg gatcggcgcg ctcagcccga cccgctccca
     9541 cggcgcggcg aaggcgcggg gcagccccgc catcacggcc atctccagca cgggctggtc
     9601 gggggagagg aggtagacgg caccggagtg ggcgtgcacc tcgtccatca gggcggccag
     9661 cgtgagcgac agcaggggac ggcccacggg ggcgtgcgac cgcgcggacg cctgtccctc
     9721 ggacacgccg accacctcct cgcacggccc gcacccaatc tcccctgcga aagggcgtcc
     9781 cgcacgacga gcgggacgcg ccgggcgcgc gcatctgtgc ggatacccca ttgcggcgcg
     9841 gcaagacgga atcagcccct ccggtgcttg aggagcgcgg tccggggcgg agccccgcgg
     9901 aggtccgggc ggcccggacc gcgcgcggtc gcggacacgt gaaccgagag ccgggcgcct
     9961 ccccgtgaga agagcggcgc ggctctgccg cgtgccgttc gcgcacaggg gctgcgtttg
    10021 tgtcccttta gcgctgtaat ggccaacgtg gtcggtgagg gcgctgcggg acgcagcacg
    10081 agaacgctgc gcgccgaagg cgcactgagc gcgctcgcgg ccggccccca cgcgaccgac
    10141 cggctgcgcc gcgtcctgga acaggccctc gtgttcgcgg gggcggcctt cgccggcttc
    10201 tacaccctcg gcgagg
//
